LOCUS       DASHWH010000001       101344 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.001, whole genome shotgun sequence.
ACCESSION   DASHWH010000001 DASHWH010000000
VERSION     DASHWH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.001"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..972
                     /locus_tag="U2R41_000001"
     CDS             85..972
                     /locus_tag="U2R41_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM8887448.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="U2R41_000002"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="U2R41_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEM8887449.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="U2R41_000003"
     CDS             2274..2720
                     /locus_tag="U2R41_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8887450.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="U2R41_000004"
     CDS             2993..4345
                     /locus_tag="U2R41_000004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEM8887451.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="U2R41_000005"
     CDS             4516..4803
                     /locus_tag="U2R41_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887452.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="U2R41_000006"
     CDS             5029..5472
                     /locus_tag="U2R41_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8887453.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="U2R41_000007"
     CDS             complement(5478..6662)
                     /locus_tag="U2R41_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEM8887454.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="U2R41_000008"
     CDS             6930..7628
                     /locus_tag="U2R41_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM8887455.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="U2R41_000009"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="U2R41_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEM8887456.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="U2R41_000010"
     CDS             complement(8438..8971)
                     /locus_tag="U2R41_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887457.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="U2R41_000011"
     CDS             complement(9268..11649)
                     /locus_tag="U2R41_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM8887458.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="U2R41_000012"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="U2R41_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEM8887459.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12888
                     /locus_tag="U2R41_000013"
                     /pseudo
     CDS             12350..12888
                     /locus_tag="U2R41_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
     gene            12978..13397
                     /gene="gpsB"
                     /locus_tag="U2R41_000014"
     CDS             12978..13397
                     /gene="gpsB"
                     /locus_tag="U2R41_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEM8887460.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13419..13786
                     /gene="rnpB"
                     /locus_tag="U2R41_000015"
     ncRNA           13419..13786
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U2R41_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13995..15161
                     /locus_tag="U2R41_000016"
     CDS             13995..15161
                     /locus_tag="U2R41_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEM8887461.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15207..16703
                     /locus_tag="U2R41_000017"
     CDS             15207..16703
                     /locus_tag="U2R41_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEM8887462.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16784..17467
                     /locus_tag="U2R41_000018"
     CDS             16784..17467
                     /locus_tag="U2R41_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8887463.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18112..19302
                     /locus_tag="U2R41_000019"
     CDS             18112..19302
                     /locus_tag="U2R41_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEM8887464.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19404..20357
                     /locus_tag="U2R41_000020"
     CDS             19404..20357
                     /locus_tag="U2R41_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEM8887465.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20426..21775
                     /locus_tag="U2R41_000021"
     CDS             20426..21775
                     /locus_tag="U2R41_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8887466.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21958..22281)
                     /locus_tag="U2R41_000022"
     CDS             complement(21958..22281)
                     /locus_tag="U2R41_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887467.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22410..23573
                     /locus_tag="U2R41_000023"
     CDS             22410..23573
                     /locus_tag="U2R41_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM8887468.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23643..24992
                     /locus_tag="U2R41_000024"
     CDS             23643..24992
                     /locus_tag="U2R41_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8887469.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25037..25468)
                     /locus_tag="U2R41_000025"
     CDS             complement(25037..25468)
                     /locus_tag="U2R41_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8887470.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25856..28498
                     /gene="alaS"
                     /locus_tag="U2R41_000026"
     CDS             25856..28498
                     /gene="alaS"
                     /locus_tag="U2R41_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEM8887471.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28698..29294
                     /locus_tag="U2R41_000027"
     CDS             28698..29294
                     /locus_tag="U2R41_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8887472.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29416..30564
                     /locus_tag="U2R41_000028"
     CDS             29416..30564
                     /locus_tag="U2R41_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM8887473.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30602..31015)
                     /locus_tag="U2R41_000029"
     CDS             complement(30602..31015)
                     /locus_tag="U2R41_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEM8887474.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31075..31524
                     /locus_tag="U2R41_000030"
     CDS             31075..31524
                     /locus_tag="U2R41_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEM8887475.1"
                     /translation="MGERLENEKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGP
                     YWKSNFVLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGV
                     QMMLQDEKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="U2R41_000031"
     CDS             complement(31623..31856)
                     /locus_tag="U2R41_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEM8887476.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="U2R41_000032"
     CDS             32039..33709
                     /locus_tag="U2R41_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEM8887477.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="U2R41_000033"
     CDS             33856..34242
                     /locus_tag="U2R41_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887478.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="U2R41_000034"
     CDS             34229..34927
                     /locus_tag="U2R41_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8887479.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="U2R41_000035"
     CDS             34932..35396
                     /locus_tag="U2R41_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEM8887480.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="U2R41_000036"
     CDS             35393..36304
                     /locus_tag="U2R41_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8887481.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="U2R41_000037"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="U2R41_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEM8887482.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="U2R41_000038"
     CDS             37092..38375
                     /locus_tag="U2R41_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEM8887483.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="U2R41_000039"
     CDS             38388..39314
                     /locus_tag="U2R41_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEM8887484.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="U2R41_000040"
     CDS             39333..40613
                     /locus_tag="U2R41_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEM8887485.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="U2R41_000041"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="U2R41_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEM8887486.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="U2R41_000042"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="U2R41_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEM8887487.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="U2R41_000043"
     CDS             44938..45714
                     /locus_tag="U2R41_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEM8887488.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="U2R41_000044"
     CDS             45711..46643
                     /locus_tag="U2R41_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEM8887489.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="U2R41_000045"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="U2R41_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEM8887490.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="U2R41_000046"
     CDS             47363..47992
                     /locus_tag="U2R41_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEM8887491.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="U2R41_000047"
     CDS             47980..48294
                     /locus_tag="U2R41_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887492.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="U2R41_000048"
     CDS             48332..48973
                     /locus_tag="U2R41_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8887493.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49016..49708
                     /locus_tag="U2R41_000049"
     CDS             49016..49708
                     /locus_tag="U2R41_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEM8887494.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="U2R41_000050"
     CDS             49705..50607
                     /locus_tag="U2R41_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8887495.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="U2R41_000051"
     CDS             complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="U2R41_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEM8887496.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="U2R41_000052"
     CDS             52661..53284
                     /locus_tag="U2R41_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887497.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="U2R41_000053"
     CDS             53734..54783
                     /locus_tag="U2R41_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8887498.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="U2R41_000054"
     CDS             54780..55661
                     /locus_tag="U2R41_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887499.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="U2R41_000055"
     CDS             55664..56434
                     /locus_tag="U2R41_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8887500.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="U2R41_000056"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="U2R41_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEM8887501.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="U2R41_000057"
     CDS             57745..58908
                     /locus_tag="U2R41_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8887502.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="U2R41_000058"
     CDS             59025..59435
                     /locus_tag="U2R41_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8887503.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="U2R41_000059"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="U2R41_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8887504.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62008..62292
                     /locus_tag="U2R41_000060"
     CDS             62008..62292
                     /locus_tag="U2R41_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887505.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62452..62688
                     /locus_tag="U2R41_000061"
     CDS             62452..62688
                     /locus_tag="U2R41_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8887506.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="U2R41_000062"
     CDS             62685..63083
                     /locus_tag="U2R41_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEM8887507.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63750..64694)
                     /locus_tag="U2R41_000063"
     CDS             complement(63750..64694)
                     /locus_tag="U2R41_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM8887508.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64990..65934)
                     /locus_tag="U2R41_000064"
     CDS             complement(64990..65934)
                     /locus_tag="U2R41_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887509.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66155..67369
                     /locus_tag="U2R41_000065"
     CDS             66155..67369
                     /locus_tag="U2R41_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8887510.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67391..69406
                     /locus_tag="U2R41_000066"
     CDS             67391..69406
                     /locus_tag="U2R41_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEM8887511.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69489..70862)
                     /locus_tag="U2R41_000067"
     CDS             complement(69489..70862)
                     /locus_tag="U2R41_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM8887512.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70888..71172)
                     /locus_tag="U2R41_000068"
     CDS             complement(70888..71172)
                     /locus_tag="U2R41_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8887513.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71174..73189)
                     /locus_tag="U2R41_000069"
     CDS             complement(71174..73189)
                     /locus_tag="U2R41_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8887514.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73368..73673)
                     /locus_tag="U2R41_000070"
     CDS             complement(73368..73673)
                     /locus_tag="U2R41_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEM8887515.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73762..74793)
                     /locus_tag="U2R41_000071"
     CDS             complement(73762..74793)
                     /locus_tag="U2R41_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8887516.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75000..75875
                     /locus_tag="U2R41_000072"
     CDS             75000..75875
                     /locus_tag="U2R41_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8887517.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76027..76869)
                     /locus_tag="U2R41_000073"
     CDS             complement(76027..76869)
                     /locus_tag="U2R41_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8887518.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76961..78838)
                     /locus_tag="U2R41_000074"
     CDS             complement(76961..78838)
                     /locus_tag="U2R41_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8887519.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78835..80256)
                     /locus_tag="U2R41_000075"
     CDS             complement(78835..80256)
                     /locus_tag="U2R41_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8887520.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80259..83648)
                     /locus_tag="U2R41_000076"
     CDS             complement(80259..83648)
                     /locus_tag="U2R41_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM8887521.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83905..85296
                     /locus_tag="U2R41_000077"
     CDS             83905..85296
                     /locus_tag="U2R41_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887522.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85369..86016)
                     /locus_tag="U2R41_000078"
     CDS             complement(85369..86016)
                     /locus_tag="U2R41_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM8887523.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86024..86710)
                     /locus_tag="U2R41_000079"
     CDS             complement(86024..86710)
                     /locus_tag="U2R41_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887524.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86716..87789)
                     /locus_tag="U2R41_000080"
     CDS             complement(86716..87789)
                     /locus_tag="U2R41_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887525.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88011..88685
                     /locus_tag="U2R41_000081"
     CDS             88011..88685
                     /locus_tag="U2R41_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8887526.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88690..90030
                     /locus_tag="U2R41_000082"
     CDS             88690..90030
                     /locus_tag="U2R41_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8887527.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90179..91165)
                     /locus_tag="U2R41_000083"
     CDS             complement(90179..91165)
                     /locus_tag="U2R41_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEM8887528.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91889..92920
                     /locus_tag="U2R41_000084"
     CDS             91889..92920
                     /locus_tag="U2R41_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8887529.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93029..94561
                     /locus_tag="U2R41_000085"
     CDS             93029..94561
                     /locus_tag="U2R41_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEM8887530.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            94700..95512
                     /locus_tag="U2R41_000086"
     CDS             94700..95512
                     /locus_tag="U2R41_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEM8887531.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            95502..95987
                     /locus_tag="U2R41_000087"
     CDS             95502..95987
                     /locus_tag="U2R41_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8887532.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            96010..96555
                     /locus_tag="U2R41_000088"
     CDS             96010..96555
                     /locus_tag="U2R41_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEM8887533.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(96628..98271)
                     /locus_tag="U2R41_000089"
     CDS             complement(96628..98271)
                     /locus_tag="U2R41_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM8887534.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(98400..100628)
                     /locus_tag="U2R41_000090"
     CDS             complement(98400..100628)
                     /locus_tag="U2R41_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8887535.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(100661..101140)
                     /locus_tag="U2R41_000091"
     CDS             complement(100661..101140)
                     /locus_tag="U2R41_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887536.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        32048 a        18131 c        20470 g        30695 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca atcacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aacaattagc agaacaatta
    12661 gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca gctaaagaat
    12721 cacactcgtt ttatacttga gcatacagtg ggctgtctgc tgatctatga tgaagaattc
    12781 cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga tcatccctac
    12841 gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc gatattgtgt
    12901 ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt acgtaaatga
    12961 aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt ttacaacaag
    13021 aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc ttagacaata
    13081 tcatcaaaga ttatgaaact tacagcaaag aactccttgc tttacaagaa gaaaacgatc
    13141 gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa acacgcgtgc
    13201 aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta aaacgcttgt
    13261 caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt gcagtcaaac
    13321 cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat gataatgaaa
    13381 aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg
    13441 acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg
    13501 cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac aagctaaggc
    13561 ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg gggaaaccca
    13621 tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag gggcgctttt
    13681 tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg ttaccgcttt
    13741 gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc gcaaacatca
    13801 gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca tttgaaagct
    13861 atttttcaat acttcagatg aatcgttctt attacaaaac aaacttttgt atcaccggtt
    13921 attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt tttttataaa
    13981 taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact gcggcaagcg
    14041 ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc caagttgaga
    14101 acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat ttatggttac
    14161 gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg tttgatgaat
    14221 tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat gcagcttttc
    14281 ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc caagcgatca
    14341 caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct tctgtaccac
    14401 tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac catgtgatgg
    14461 taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg
    14521 gagcaccatt gaaagaaaac atggcagcag cacttattat gttgacaaat tggcgaaaag
    14581 accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa gcagccttaa
    14641 taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct tgggattggt
    14701 tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca gattatgata
    14761 ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt gccaaagcca
    14821 atgcagaaga aatcggatta ggctcttcga ttacctttaa gcaacaagca gtcaaagact
    14881 tcaaaacgga taaagaatac ggggtcatcg tggctaatcc gccttacgga gaacgtctag
    14941 gagaagaaga aactgttcgg aaattatata aagaaatggg cgatgtattc cgtccattaa
    15001 aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt tacggacaaa
    15061 aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta ttccagtatt
    15121 ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa ttgattcatt
    15181 caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa gaagtaaaag
    15241 aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa acaggtatgc
    15301 ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc atttattttt
    15361 caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa catccggaag
    15421 aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat gatttggaac
    15481 atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca gcacacggac
    15541 aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca ttgaatcaaa
    15601 atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag acgaattatg
    15661 atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat aaggtatttg
    15721 atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac
    15781 ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga tttgtagtga
    15841 aagtaatcga acaacttggt tatgatttta gtcgtggtcg tttagatgac actgtccacc
    15901 catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg aacgaaacag
    15961 atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg tatgaacaag
    16021 acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg ggtatccatg
    16081 aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc tggaaaaagc
    16141 aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct tttgacaaat
    16201 tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca gacagtttga
    16261 cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc aatgaagggg
    16321 tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat ctgggaatac
    16381 gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt gcaagctttg
    16441 gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt cattctttgc
    16501 agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca atcaaaaact
    16561 ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa ttaatcatgg
    16621 atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa gacctgtatt
    16681 ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat tcatctaatc
    16741 tatagatgtt ttcagctatt tacttaagaa agaagtggaa cgaatggata aattacaagt
    16801 gatcgctgat tatagtatgg accaattagc gcatgatcaa acaggacatg ggagcgatca
    16861 taccaaaaga gttgtcaagt tagctgaaaa aattttggag acggaaccgc aggcagaccg
    16921 ctttgtgacg cttgcagcag cttacctaca tgatacgatt gatgataaag tcgttaaaaa
    16981 tgagaacgag gcaaagcaac agcttcgtgt attccttcgc tctttgccaa tagtagagga
    17041 acagatctcg atgatttttt ccattatcga aaatatgtcc ttttctaaaa atctctctga
    17101 agcggccgaa ctatctctgg aaggtaagat cgttcaagac gctgatcgta tcgaagcatt
    17161 aggagcaatc ggcattttgc gtactgctta tttcggcggt ggacatgggc acccgatttt
    17221 tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa aaggcacgac
    17281 agtgatcaat catttttatg aaaaactgtt cttgctgtct gataaaatga atactgatta
    17341 cggatatgaa gaagccaaac gaagagaaac cttcatgcgt gaattcttag aagaattttt
    17401 tctggaatgg tctattgacg atgaaaattt tactccagac aattggatca aaaataacaa
    17461 aaaatgatgg aggttgtgac agaagtggtc agctccaaga tatttcggat cattttccgt
    17521 aaaagttact tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag
    17581 tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga tgaatattgt aacttgtgat
    17641 tcggaattgg caggagaaca ccgtttattc ggaagaatag gactgtgaca agacttttgt
    17701 cacagtcctt ttttcgaatt caacaagtaa aacttttaag aaatattcag agagtaaaat
    17761 taaaaattgc tgataaacga agatggaaat tttatgtatt tttattggtc tatgcaataa
    17821 ataaattaaa tattcagaat acaattgatt ttaacgaaag ggcttctatc aatggttgtg
    17881 ttgtatttat ctgcccaggt agagtaaaat agatcattat ctaaaaggta attgttcata
    17941 ctatataagt catttgaaag taaaaagata ctgtaaataa aaaatgtggt taaataattc
    18001 tcataatcag tgtaagcgtt atcaaaaata tagcatactt gctttgtaga tgaatgacac
    18061 gatatatgtt tggtatgttc tgcaagacag atgaatatgg aggtggagac aatgtcttcc
    18121 aaagaactaa caatgtattt agagaaagaa ttggatcaac tgaaagaaaa ggggttatac
    18181 aatacgatcg acatactaga aagtgaaaat ggtgcttgta tcatcgtaga cggaaaaaag
    18241 atgatcaatc tagcatccaa caattatctt ggatttgcca atcgcgaaga attgaaaaaa
    18301 gcatgtattg aagcaaccga aacttatggt gtaggagcag gagcagtccg aacgatcaat
    18361 ggttcgctga aaatccatca gcaattagaa gaaaaaatcg cagaattcaa agggacagaa
    18421 gcagcaatcg cctttcaatc tggattcaac tgcaatatgg gcgctatctc tgcagtaatg
    18481 acaaaagaag atgctatctt atcggatgaa ttaaaccatg catctattat tgacggttgc
    18541 cgcttatccg gagcaaaaat catccgaatt aaacatcaag atatgaaaga ccttgagaaa
    18601 aaagcaaagg aagcgataga aagtaagaaa tataaaaaaa tcatgtatat cacagatggt
    18661 gttttctcca tggatggaga cattgcacga ttgccggaaa ttattccgat tgttgaaaaa
    18721 tatggactga tcacctacgt cgatgatgcg cacggctcag gcgtaactgg aaaaggtgca
    18781 ggaaccgtca agcattttgg attaagcgac aaaatcgata tgcaaatggg cacgttatcg
    18841 aaagctatag gtgttgttgg gggttatgta gctggatcga aaacactaat cgactggttg
    18901 aaagctagaa gtcggccatt cctattttct acttcgctaa ctccaggtgc agctgcagct
    18961 gcattggctt cgattacttt gatgcaagaa catccggaac ttgtcgaaaa agtttgggaa
    19021 aatgctaatt attttaaaga agaattgaaa aaagtaggct ataacatcgg tatgtctgaa
    19081 acacctatta ctccagtcat tttgggagac gaaaaagtga ctcagacttt ctctaagaag
    19141 ctgatcgaac atgggattta tgctaaacca atcgtttatc caactgtgcc tcttgggaca
    19201 ggcaggatca gaaatatgcc gactgcggaa catacgaggg aaatgcttga tgaagcagta
    19261 gcagtttatc aaaaaatagg aaaagaaatg gaaattattt aactaaattt agttttttcg
    19321 tctatttaaa attagagaac atgttcacgc atatattgtt aatcatctga agatagtagt
    19381 aaaaaagatc agggaggatc atcatgaaaa aagtattggt gacaggatgt ttaggacaaa
    19441 ttggttcaga gttgatcaca cggctacgtc aagaactagg cagtgaaaat gtcattgcga
    19501 cagatatacg catgcctgaa gtcattgaga aaaacgatat ctttgagatc ctagatgtaa
    19561 tggattacga aaaaatgcgc agcatagcag aacaatatca agtggacaca ttgattcatt
    19621 tggctgctct tttgtcagct gttgcagaag caaaaccaca attagcctgg aatttgaacg
    19681 tgactgggct ggtcaatgca ttggaagtcg ctagagaatt ccagctgaaa ttttttacgc
    19741 caagttcaat cggcgcgttt ggtccaaata ctccaaaaga tcatacccca caagatactc
    19801 ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc tggagaacta ctatgtgatt
    19861 attattatgt gaaatatggc gtagatactc gtggtgtccg tttccctgga ttgatttctt
    19921 atgcaacctt gccaggaggc ggtacgacag actacgcagt cgatatttat tattcagcaa
    19981 tcaaagaagg aaagtacact agtttcattg atcaaggtac atcaatggat atgatgtata
    20041 tgccagatgc gatcgatgcc attataaaac tcatgaatgc agatcctgat cgattggttc
    20101 atcgaaatgc cttcaatata tcggcgatgt cttttgaccc ggaagagatc aaagcatcta
    20161 ttcaaaaaat cataccagat tttgaaatgg attatgatgt ggatcctgta cgtcagtcaa
    20221 tagcagaatc ttggccaaat tcactagatg tttcttgtgc gcaagaggaa tggggctttt
    20281 cgccgaaata cgacttagat gtgatgacaa aggatatgtt ggaaaaactg aaagaaaaat
    20341 tggtagagac ggtataaaac aagaaccaca tccaaaaagt tttttatact tttgggattg
    20401 gctatggtat gaaaggggac aagggatgga agaatatcaa gccacgccag taaaagaagt
    20461 aaaaatagaa aatgaattaa aacgtacgat gggatttttt actgctttat cgactgttat
    20521 gggcacagtg atcggtgcag gggtattttt taaagccgca agcgtagcag aagttacagg
    20581 atccgccagt ttgcatatgt tttcttggtt tctcggtggt atgatttctg tctgtgcagg
    20641 actaacagga gctgaactgg cagctgctat tccagaaaca ggagggatga ttaaatatat
    20701 cgaacggatt tacggaaata cagcagcttt tttattgggc tgggcgcaag tcgtgattta
    20761 ttttccagca aatgtagcag ccttgtcgat cattttcggt acacagttcg tcaatctatt
    20821 tggtctttcc cagtcaatga ttgttcccgt agctgttaca gctgctgtat cgatcctgtt
    20881 gatcaatttt ttaggttcta aagctggagg ggcttttcag tcgattacgc ttgtctgcaa
    20941 gcttattcct ttgtttgtga tcgtcatttt cggtttgttc cgacaagaag gagtagactt
    21001 ccaactgttt ccgatccaag caggagaaaa tctttcattt ttctcagcct tgggagcggg
    21061 cttgctagca acgatgttcg catacgacgg ttggatacac gtaggaaata tttcgggaga
    21121 attgaaaaag ccggcgaaag atttgccaaa agcaatttct ttaggaatca tcggaatcat
    21181 gatcgtttat ttattagtaa atgcggtgtt cttaagaacc gcatctatcg acggagtcgc
    21241 aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt tttggcggct tcggtggaag
    21301 actggtcaca gttggtatct tgatttctgt ttatggaacc atcaatggct atacccttac
    21361 tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac ctgcctttta gcaagctctt
    21421 tgctaaactt catgataaaa ctaaagttcc tgtagccgca ggtatcttag agctagtcat
    21481 cgctattggc atgatgatga tcggtggttt tgatacacta acggatatgc tgatctttgt
    21541 catttggatt ttttatacga tggtctttgt tggtgtcatt cttcttcgta aaaaagaacc
    21601 tgatttgttt cgaccataca aagttccaat gtatccgttt attccactag tagcaatcat
    21661 tggagggact tttatcgtat caagcacgct tatcactcag acctttttag cttcgatggg
    21721 gatagtattg acacttgctg gtatcccgat ttatctctat ttaaaacgta gatagctaaa
    21781 acattcgaat tttattagaa ctgcacttct ctgccaaaca aaatatttta tttaaatgag
    21841 gagtacgtgg aagaaatgtc aaaagtactc gttaattttg atcacgtaaa tgtcgttttg
    21901 tctacgatga aatagcaaag taaaagacag aaatcgggct caaccgattt ctgtctttta
    21961 ctttgatttg cgtaactgaa ctattcggcg tacgccgcga taaatattta ataaaaccaa
    22021 gcaaccagaa atgaaaatgg gaagccaaat cagatagaca gggtagccaa gcactttaga
    22081 caaagcatgt ggagtcattt tatccatttc aagaagtgct tgctgccaag tctgaagatt
    22141 gaagactagc atgaaacccg aaataatcag gagaataaat cctaaataat gaattgtagt
    22201 tctttttctt acataagcga tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa
    22261 aacaaagtac atattcagca tctcgacacc ccctttgtgt ttatcttact ttactttgaa
    22321 agaaatgaga aataatagcc tcgttacttg cttgaaacag caaaggactg ctaagataag
    22381 agagatacaa agaatcaagg aggagtcata tggcaagtta tatcgaaccg attcgttttg
    22441 cgctgattgt ttttccattt ttagccttag cgatctcatc tgtttttttt atttatgaat
    22501 atcgaaagta tggaacattt ctagtaacaa gggcagttat tttgtattct ttcgtttttt
    22561 atttattgtg tgcgtatttt ttggtgatct taccacttcc ggcaaaggag acggttgccc
    22621 aaatgacagg gccaaagatg gagctgcatt tatttgcttc atggcaacat ttcagagaca
    22681 acacggtatt ggtcttgtct gaacctagta cctacctgcc ggcaatgaag cagagtgtat
    22741 ttttggaacc agcttttaat gttttattac tcataccatt tggcatttat ttaagatatt
    22801 atttccggct ttcttttatg aaaacgatga ttgctagttt ctgcctttct ctattttttg
    22861 aattgacgca gctaagtgga ctttatttta tctacccaag accataccgc ttatttgatg
    22921 tgaatgattt agtgacaaat acattgggtg gaatcattgg ttttgtcgtt acgccgttat
    22981 ttacatttat gcttcctaca agaagacgaa tggatgaact ttcttatgaa aaaggaaaga
    23041 ctgtctcatt gactcgacgc ttggtcgctt atttgatcga ctggacattt ttatctctta
    23101 ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga ttcaggggca gttacttctc
    23161 atatctggtg gcttttatta gaagtagggg tatactttat gattgtaccg tttataacca
    23221 atggatggac acttggcaaa aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac
    23281 gaatttcttt aaaagcattg atgatccgct acgggtatct gtactttctt ttttatggtt
    23341 taagctggtt ggggcagttg cttcagcaaa tcatgaattc gacaacgaat acggtgttgc
    23401 agatgagtgc agtattcatt tctctcggtg tcttgttact acaaggtatt tttattttaa
    23461 atatcttgct atcatttttg agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca
    23521 catatactgt tagcacgatt ggccaaacaa aggatgatga ttctcgaaat tagctgagca
    23581 aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa tttaaagaga agaggtgcac
    23641 gaatgaataa ttttgatata ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa
    23701 agggcttcaa acagcccaca gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa
    23761 aaaatatcat tgggcaatct caaactggta caggaaaaac acatacgttt ttgttgcctt
    23821 tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt tgttattaca gctccaagta
    23881 gggaactagc tgcacagatc tatgaagcag ctacacaaat cgctaaattt tctacaccgg
    23941 agatccgtgt ctcgaatttt gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc
    24001 atcaccaaca gccacaggtt gtagtaggca cgcctggtag aatattagac atgatgaatg
    24061 aacaagcgct gaagattcac accgcatttg catttgttgt cgatgaagcg gatatgacat
    24121 tggatatggg ttttttaaat gaagtagacc aaattgcaag tcgattacca gaaaagctgc
    24181 agtttttagt tttttctgcc actattccag aaaaacttcg tccattctta cgaaaataca
    24241 tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt tatttctgaa gacattgaaa
    24301 attggctgat ttcaacaaaa ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa
    24361 ttggtcatcc ttatttagca atcgtatttg ccaatacaaa acagcatgtg gatgaaattg
    24421 ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat tcatggagac atcaccccaa
    24481 gagagagaaa acgagtgatg cgacaagtac agaatctgga gtatcaatat gttgtagcga
    24541 ctgatttagc tgctagaggg atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa
    24601 ttccagaaga tctagacttc tttatccatc gtgtcggacg tactggacgt aatggcttaa
    24661 aaggaacggc cattacgtta tattcgccaa atgatgagca agccattgat gcgatagaaa
    24721 aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg
    24781 atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt attagatccc acgctgattg
    24841 gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta taagaaaaaa atcgattggg
    24901 caattgcaga aaaaaataaa aaagaacgca aaatcgaacg cagacagcaa agccgtactg
    24961 ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg
    25021 tcacaatctc ttgtttttaa gctagacttt ttttcattcc gatacaaagt acttcatctt
    25081 caataaaagg ttctgtgcaa gtatgatacc cttgtttttc ataaaaatct acagccgtca
    25141 tttcagctac tacataacta gtctgacaac cttctgaaac cgcccgtttt tcagcagctt
    25201 caagcaactg accgcctaac ccttttcctc ggtattcttt tgctacgcaa aatcgttccg
    25261 gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt agctactggt cttttttgat
    25321 caagaaatag ataataggtt tgttgcggga tatcttttcc gtcgaattct agttctgcag
    25381 gaatcccttg ttctaataca aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc
    25441 attcatccgc tccaactatt tttttcatca tattcctcct tgtcatgaat agcagagatg
    25501 tattgattcc tttattctta tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa
    25561 aataaaaatt gagatattga cagtgatcaa tagaatgcgt atactaaata agtacgaaaa
    25621 ggatatgatt tgttttttaa gactcacaga aaattctttc ttggctgaaa aaagaatagt
    25681 cactaatcaa attgctccct ttttctattt tggtagaaat accgacgcag ctcagcgtta
    25741 tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg
    25801 tccttggctg tgatggtgtt tttttgtatt taatttatag aaagcaggta atgacatgaa
    25861 acaattaagt agtgcacaag ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca
    25921 tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa
    25981 ctctggtgtt gcaacattga aaaaatattt tgatggttca gttgtgccag aaaatccaag
    26041 aatcacaaat gcccaaaaat ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc
    26101 acggcaccat acgatgtttg agatgctggg taatttttcg attggtgact atttcaaaaa
    26161 tgaagccatc cactgggcat gggaattttt gactgatcca aaatggatgg cattcgatcc
    26221 agaaaaactt tatgtaacag tttatccaaa agatacagaa gcaaaacgca tctggcatga
    26281 agaagttggc ctgccactag atcacattat tgatgtagaa gacaacttct gggatatcgg
    26341 tgcaggccca tgtggtccgg atacggaaat tttctatgac cgtggagaag cttataacga
    26401 tttagcagaa gacgatcctg aaaactatcc aggaggagaa aatgaacgtt atttagaaat
    26461 ctggaactta gtattttcag aattcaacca taagcctgat aacacatttg aaccacttcc
    26521 tcataaaaac attgatacgg ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc
    26581 gtcaacgaac tttgagacag acttgtttat gcctattatc cacgccgtag aaaagctaag
    26641 caaacaagta aaatatggtg aaaacccagt aacagatatt tcttacaaag taatcgcaga
    26701 tcatattcgc gccctttcat ttgctattgg cgacggtgca ttgccatcga atgaagggag
    26761 agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat
    26821 tgaacaagca ttcttatatc gtttagtccc tgttgttggg gaaatcatgg taagttatta
    26881 tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt atccgtacag aagaagaacg
    26941 tttcaatgaa acaatcaatg aaggtttgga aatcttaaat caagtgatcc aagaagtaaa
    27001 aaacgaaaac gggacgactc tagcaggaaa agacatcttc aaactatacg acacatatgg
    27061 cttcccggta gaattaacgg aagaggtggc agaagatgaa ggactgaaag ttgatcatgc
    27121 tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct cgctctgccc gcagtactga
    27181 aaaatcaatg ggggtacaat ctgctttatt gacagatatc aaagtagaaa gtaaattcat
    27241 tggatacaca gaacaagaat cagatagtca attattggtc atcgttaaag gtgagtctct
    27301 tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt gatcaaacac ctttctatgc
    27361 agaaatgggt gggcaagtag cggatcaagg atggatcatg aatgaagtag gcgaaatcgt
    27421 agcagttgtt acagatgttc aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt
    27481 aaaagcacca atgagtgaag gacaaaccta tcatctattg atcgatcaat cgatgcgcaa
    27541 tcggatcatc aaaaaccata cagcaaccca tctactgcac aaggcattga aagaagtact
    27601 tggcgagcat gcaaatcaag ctggttcttt agtcgctcct ggacatttac gattcgactt
    27661 tacccacttt ggtcaaatca cttcagatga attagctgaa atggaaagaa tcgtcaacga
    27721 aaaaatctgg gaagctatcc cagttgaaac aattgaaaca gatatcgata cagccaaaaa
    27781 catgggtgca atggctttgt ttggtgaaaa atacggacat gacgttcgag tagtaaatat
    27841 tggcggctgg tcaatcgaac tgtgcggagg cacacatgta gcaaatacag aaaacatcgg
    27901 aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt
    27961 aacaagtaaa gaagcgtacg aactacttga agaagaagaa aaacaactaa aacaagtagc
    28021 aacaattgtt aaatcacctc aattgaaaga agttgtttca aaagcggaac aattgcaaca
    28081 gcaagtaaga gagctacaaa aggaaaatga acaattagct agcaagttag cgaatcaaca
    28141 agctggtgat gtattcaaag atccagaaac gatcaatgga aaaacaatca ttgcagcaca
    28201 agtaaaagtg aaagatatga atcagctgcg tcaattagct gaccaatgga aacaaaaaaa
    28261 tatttcagat attcttgttt tagcaaacga acaagaagga aaagtcagtc tgctagcggc
    28321 aatgacgaaa gaatcaaatg aagctggact aaaagctggc gacttgatta aagcaatcgc
    28381 accgaaagta ggcggtggcg gtggtggacg tccagacatg gcacaagctg gcgggaaaaa
    28441 tcctgctggt attccagatg cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg
    28501 cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa cgaaacatga ttatgggggc
    28561 gaagacaaac aagtcttgcc ctcatacttt tttcaattgg atggcgcatc taatcaaatg
    28621 gtcggattcg gatggtcttt agttgacgaa agtgacagaa atttttacga ttagtataaa
    28681 taaaaagggg tgaaaaaatg aatagtagaa ataagacctt tcgactagtc ttacgtgcga
    28741 tcttgttagc aatcatcatc gtacaagcga tggttccttg gctggggttt attccattag
    28801 gattcattag tttaacaatt atccatatca cagtaattat tgctgcagta gtattaggcc
    28861 caaaagatgg catggtgatt ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg
    28921 ccatgccaac aacgccattt gatacattag tgttcacgaa tccgattatc tcggtagttc
    28981 ccagagtact tgttggctta gtagcaggct tggttttcca ctggatttat caaaaaaata
    29041 aatcaataac gatcagctca gcctttgcag gggtactggg ttcattggtc aatacagtct
    29101 tagtgttagg attcatggga ttattttata caggtgcaac ggcagatgcc tatggtgtag
    29161 actctgctct tttatttaaa accttagcag gggttgcagc aatcaatggg ataccagaag
    29221 caataggtgc gggaatcata acaccattac tagcaaaagc gttatttgca gcaacaccgt
    29281 tgaaaccaga ataatgacca tcttttaata gtaataggac gcacgataga tagaaaaagg
    29341 aagagaggag aggaaaacaa tcgtcctctc catcttttta cctgagcaac aagcaaacta
    29401 gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa agaaacaaat accgcaacag
    29461 cggacaaaaa aaggagtact agcgggtatc ttaggattgg ttacgaatct gatcttgttt
    29521 gtctctaagc taatgatcgg gcttttttca ggaagtgtct ccattatggc tgatgcagtg
    29581 aacagtctat cagatactgc ttcatccata ttgactttga tcggatttaa aattgctgca
    29641 aagccagcag atcaggaaca tccttatgga cacgaacgct tcgaatatat cagtgggtta
    29701 tttgtttcga tcatcattac ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa
    29761 atcatccatc cagaaaatgt ctctttaaca cccatcgtct tttttgtcct aatcatttcc
    29821 atctttttga aatttgcaca aggcaaaatg tatcaggtta ttgcgcaatc cattgattct
    29881 gaaacattga gagctacagc aacagatagt tataatgatg tctttactac actggctgta
    29941 ttgctttcag cagcaatcga atgggtaact ggatggcgta ttgatggata tataggtttt
    30001 gctttagcat tatatatcct ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa
    30061 ctattaggaa gtcgcccaac gccagcagag attgaagaaa tggaagagca tttaaatgct
    30121 tgcgatgata tacttggcta tcatgattta ttggtgcata actatggacc caataaacgc
    30181 tttgcatcag tccacatcga agtagatgaa tcaatgaatt tgaatgtggc tcataagatt
    30241 attgatctga tagaaaaaga ctttaaaaag tctttggatg tggaacttgt ctgtcacttg
    30301 gatccaatgc ctgttgataa cgaaaaatac tataatatct tgcggcagtt aaagcaaatt
    30361 cttcttgaaa caggtacagg gcttaatatt catgatttta gagtaattca tcaaggaaaa
    30421 acgctgcaat ttgatgtagt ggtccctgag aaattccagt ttgttgatac agaactagaa
    30481 agaatcttac gtagtcaaat agaagaaaaa atcggagatt accaattgga aattacgttt
    30541 gatcacaatt atcttttata ttaaatgaga aactataaaa agactcctta aagtttttaa
    30601 tttaaagagt ctttttttct tttaattgtt tatggagagc ctgacgtgca agattatctg
    30661 ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa
    30721 gttcttggat tctttgaagg tagggaagga agagctcgtt cttcgttgcg tttttgtcga
    30781 ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt
    30841 ttttctttag aaattctaaa gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc
    30901 ctatagaaag cgggatagat agctgttctt gtttaccttg atagacaatc aagattccac
    30961 ctccactagg accagggttg ccttttgtag aggcatcaat gtatactttt aacataaaaa
    31021 gagattctcc tttttgttca aactaatgtt atggtaagat tatatcatta ctgaatcggt
    31081 gagaggttag agaatgaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tttttcttac tttttttgtc gtttaattga atgataaaaa ccagtttctt tattcagtta
    63481 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
    63541 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
    63601 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
    63661 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
    63721 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
    63781 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
    63841 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
    63901 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
    63961 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
    64021 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
    64081 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
    64141 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
    64201 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
    64261 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
    64321 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
    64381 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
    64441 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
    64501 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
    64561 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
    64621 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
    64681 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
    64741 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
    64801 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
    64861 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
    64921 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
    64981 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
    65041 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
    65101 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
    65161 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
    65221 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
    65281 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
    65341 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
    65401 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
    65461 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
    65521 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
    65581 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
    65641 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
    65701 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
    65761 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
    65821 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
    65881 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
    65941 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
    66001 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
    66061 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
    66121 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
    66181 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
    66241 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
    66301 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
    66361 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
    66421 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
    66481 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
    66541 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
    66601 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
    66661 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
    66721 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
    66781 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
    66841 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
    66901 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
    66961 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
    67021 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
    67081 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
    67141 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
    67201 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
    67261 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
    67321 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
    67381 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
    67441 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
    67501 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
    67561 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
    67621 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
    67681 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
    67741 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
    67801 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
    67861 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
    67921 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
    67981 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
    68041 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
    68101 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
    68161 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
    68221 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
    68281 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
    68341 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
    68401 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
    68461 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
    68521 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
    68581 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
    68641 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
    68701 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
    68761 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
    68821 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
    68881 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
    68941 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
    69001 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
    69061 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
    69121 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
    69181 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
    69241 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
    69301 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
    69361 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
    69421 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
    69481 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
    69541 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
    69601 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
    69661 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
    69721 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
    69781 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
    69841 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
    69901 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
    69961 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
    70021 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
    70081 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
    70141 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
    70201 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
    70261 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
    70321 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
    70381 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
    70441 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
    70501 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
    70561 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
    70621 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
    70681 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
    70741 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
    70801 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
    70861 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
    70921 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
    70981 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
    71041 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
    71101 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
    71161 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
    71221 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
    71281 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
    71341 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
    71401 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
    71461 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
    71521 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
    71581 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
    71641 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
    71701 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
    71761 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
    71821 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
    71881 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
    71941 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
    72001 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
    72061 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
    72121 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
    72181 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
    72241 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
    72301 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
    72361 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
    72421 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
    72481 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
    72541 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
    72601 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
    72661 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
    72721 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
    72781 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
    72841 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
    72901 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
    72961 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
    73021 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
    73081 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
    73141 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
    73201 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
    73261 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
    73321 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
    73381 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
    73441 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    73501 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    73561 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    73621 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    73681 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    73741 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    73801 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    73861 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    73921 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    73981 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    74041 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    74101 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    74161 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    74221 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    74281 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    74341 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    74401 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    74461 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    74521 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    74581 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    74641 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    74701 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    74761 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    74821 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    74881 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    74941 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    75001 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    75061 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    75121 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    75181 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    75241 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    75301 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    75361 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    75421 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    75481 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    75541 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    75601 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    75661 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    75721 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    75781 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    75841 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    75901 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    75961 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    76021 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    76081 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    76141 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    76201 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    76261 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    76321 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    76381 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    76441 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    76501 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    76561 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    76621 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    76681 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    76741 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    76801 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    76861 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    76921 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    76981 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    77041 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    77101 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    77161 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    77221 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    77281 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    77341 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    77401 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    77461 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    77521 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    77581 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    77641 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    77701 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    77761 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    77821 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    77881 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    77941 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    78001 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    78061 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    78121 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    78181 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    78241 taccgatttt tgtaactttc aacgttccat cattacagac tgtattttta gggtagagat
    78301 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    78361 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    78421 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    78481 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    78541 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    78601 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    78661 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    78721 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    78781 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    78841 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    78901 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    78961 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    79021 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    79081 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    79141 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    79201 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    79261 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    79321 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    79381 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    79441 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    79501 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    79561 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    79621 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    79681 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    79741 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    79801 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    79861 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    79921 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    79981 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    80041 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    80101 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    80161 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    80221 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    80281 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    80341 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    80401 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    80461 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    80521 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    80581 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    80641 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    80701 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    80761 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    80821 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    80881 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    80941 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    81001 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    81061 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    81121 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    81181 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    81241 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    81301 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    81361 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    81421 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    81481 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    81541 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    81601 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    81661 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    81721 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    81781 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    81841 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    81901 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    81961 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    82021 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    82081 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    82141 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    82201 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    82261 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    82321 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    82381 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    82441 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    82501 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    82561 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    82621 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    82681 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    82741 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    82801 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    82861 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    82921 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    82981 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    83041 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    83101 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    83161 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    83221 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    83281 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    83341 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    83401 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    83461 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    83521 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    83581 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    83641 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    83701 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    83761 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    83821 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    83881 ttgttggaat gcaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    83941 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    84001 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    84061 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    84121 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    84181 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    84241 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    84301 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    84361 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    84421 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    84481 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    84541 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    84601 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    84661 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    84721 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
    84781 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    84841 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    84901 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    84961 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    85021 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    85081 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    85141 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    85201 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    85261 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    85321 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    85381 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    85441 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    85501 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    85561 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    85621 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    85681 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    85741 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    85801 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    85861 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    85921 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    85981 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    86041 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    86101 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    86161 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    86221 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    86281 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    86341 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    86401 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    86461 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    86521 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    86581 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    86641 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    86701 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    86761 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    86821 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    86881 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    86941 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    87001 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    87061 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    87121 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
    87181 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
    87241 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
    87301 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
    87361 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
    87421 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
    87481 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
    87541 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
    87601 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
    87661 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
    87721 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
    87781 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
    87841 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
    87901 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
    87961 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
    88021 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
    88081 tcggcgagat aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
    88141 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
    88201 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
    88261 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
    88321 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
    88381 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
    88441 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
    88501 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
    88561 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
    88621 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
    88681 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
    88741 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
    88801 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
    88861 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
    88921 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
    88981 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
    89041 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
    89101 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
    89161 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
    89221 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
    89281 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
    89341 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
    89401 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
    89461 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
    89521 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
    89581 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
    89641 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
    89701 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
    89761 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
    89821 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
    89881 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
    89941 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
    90001 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
    90061 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
    90121 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
    90181 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
    90241 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
    90301 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
    90361 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
    90421 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
    90481 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
    90541 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
    90601 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
    90661 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
    90721 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
    90781 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
    90841 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
    90901 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
    90961 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
    91021 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
    91081 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
    91141 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt
    91201 atattgttac ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt
    91261 ggaatttgat taaaacaatt taattgaaat ttgaaaacat ttatgttaat gaaagtcata
    91321 ttctaataaa atgtcatttg tttctatatg aatttttatg aaaaatttca tataatttcc
    91381 actcgttgtg atggtttaat ttatccaatc catcaataag caaacaactt atataaaaaa
    91441 ggattaattc tcaatataat tgctcctacc atagaataaa tcttcatgta aagtaatgaa
    91501 gtaccaacta tttctaatac ttctttaaga attatcccaa caatatcgtt agacaaacca
    91561 ttattgaata aatggagagc caatcgattc tgaaaaaatg ttttctaatt tttcggaaat
    91621 aaaagaaaga gcctcctgcc attagaacga accaaatcca tttcattgct catgaagtaa
    91681 ccacatagct aaaaagagga taaatcaatt aaaagatcaa aagaatgact gcactaataa
    91741 aataactaaa aatgtatctt cttacttcat atcttttagc tagtacttag ttaaaaattt
    91801 gcttcctaag atcaagacaa actgtctatc ttcatttgat gaaatgcggt aaaatataga
    91861 aaatgaacac tattggaggg aatttgtaat ggacagcaaa actagaaaac aactgcaaga
    91921 aaagttaaag caacgtacac acttgctccc ttccgtcttt caacagtttt tagaagaaaa
    91981 tcgttcttcc ctttcattag gcagtcgcta tgaatacgca aaagattttc atctgtttac
    92041 tgattatttg agaaaagtat tttctgaaga agtggatgat cagctgatta tccatttaga
    92101 aaaacaagat tatcagaatt tcttagctaa aatctatcga catactcgtt cgttccaaac
    92161 tacagcgaac caagaaacgg agcaattgtt tgaaaatagc tattacggga tttcaaggaa
    92221 gcaatattca ttaaatcatt ttttacgatt tttgtatcaa aaaggactga ttgaagaaga
    92281 gatttctctt ttgggtacaa gtttaccgga aacaaccaaa gcagctccgc gatatatcga
    92341 tgcagaactt ttcaaagatt ttgagcattt gtttatcccg attgaaggct ttcaaacaag
    92401 cagagaagct agctttgaag aaaagaatag accgaggaac cttgcaatta ctgccattct
    92461 tctgtattgc ggattaaaga tccatgaagt agtagaactt aaacgtaaag atgtatcttt
    92521 gaccaagcag attcttaatc tgaatagaaa agaaaacaga ctgaataaag taccaatacc
    92581 aaaagaagcg atgccatttc tggagaacta ttttaagtta acagcacaaa atacggacga
    92641 aaattctttt gtttttcgtt cctcacatga tcagcaaatt tctcctcgga ctattcgcca
    92701 aattttgagc aaaattacag tatataaaaa cgtatcgcca gaactatgca gaaaaacata
    92761 tcgttattat gcagaactat atttagatga tgctgacgcg gtaaattatt tatgtggtaa
    92821 tcgatacctc tcatcccatt cttttcaaga tatctgggaa aagatggttg atttttctta
    92881 tcacgaatta gcggcattcg ttcgggtttc ttccatataa aacaaatgta tgatcttttt
    92941 ttcacaataa actatgttat tatataaagg attatgttac gattatcttg aatttgtata
    93001 ttacttaaca aaagataatg gagtgtgaat gatggagacc ataacgtatt cccctttgaa
    93061 cttatttacg aattttcaag aagctgcagc taacttttcc gagaccccga ttatctttga
    93121 tgagccactt tcagctttcc cagaattagg acttgaaaca acttacaaaa aatgctgcga
    93181 aagtgtgttg aaacgggctt atcaattggc tcatcttgga gtgaaatccg gagacaaagt
    93241 tattatttat aagagttccg cttttgacac ataccttcta gctgtttctg tgtcttacct
    93301 tggtgcagtg cctgtcatga cttcttatca tctaccaaca acaacaatgg aagtatttat
    93361 cgatcgttta gaagaccctt tcattttatt tgatgatgag acaaaaaagc gagtgagaga
    93421 aatttcaaat ggaacaaaat caaaacagat tcctattctt tatttaatgg aacaacctgc
    93481 tttgtctgtt tctcaatcat ttttagacaa aaatgaaatt tcttatatga cccatacttc
    93541 tggtacaact ggtatcccta aacttatttg tcattctgct cattctatgg gatggcggac
    93601 gaaatggcaa aaaacagttt tcacaaaaat ttcggaaaag aaattaatcg cttttcacat
    93661 ctctccagtg cattccagat tcaatattgg tatctcttct ttgatgtcca tgggatttcc
    93721 tatgatgcct ttagcaaacg cacaaagttc aaaagttgtt catatgttgg aagctcaccg
    93781 gccaattgca ctagagaccc atccgaataa ctttgttcag tggcgtttta ctgctaagga
    93841 gcatccagaa gctttttcgg gtatccgata ctatcactcc acttttgatg ctataaacaa
    93901 ccagacaatg aagacatttt tgcgaacgtc gatggcacag gatgcggtat ttcttcaagt
    93961 atatggccaa agtgaatgcg gaccaatgat tcttaaggct catacactag agagtttgaa
    94021 aacaagtgat gcaagagata tgggcgttgg actggaagac atgacaagcg ctcggattac
    94081 agattcagtt gggaatgtcc tacctgaaaa cacagatggt catatacaat tactgtctaa
    94141 aggacgggct ttgacttatt acaaggaaga tgcacggttc caggaaaatg tttatggcga
    94201 ttggtgggat agcggagatt acggttttat ggataagcat ggccacctat ttttgaaaga
    94261 tcggcaagtg gatttgatcg aaacgatcaa tagtacatta gctattgaag actttttatt
    94321 agattcgtta gattttttgg cagaagtagt gattgtaagg gataaaaatc attctccaca
    94381 accgattatt gctttagctc ctgataaaga aatggattgg aatcgatggt gggagcaagt
    94441 ccatgaactt cctcgcttga acgtccctat catccgtgat tttgatgcaa tcccgcgaac
    94501 agctacaatg aaagtgcaac ggcttcaaat cgagaaagaa ttaaaatcac aaaagtccta
    94561 actatttaag gaaagaaaga tccattttgg tctttctttt ctttttgttt ttttaaattg
    94621 gatgagataa aaatatgcca attggctgtt tttctttttt gaaaatttcg ttatactctt
    94681 attcatggag gaaaatgtta tgaaaagaat cttgacaatt ggcggttctg atccatttgc
    94741 tggtggagga atccaaacag atttaaaaac ttttgagaat tttggtctat tcggactaag
    94801 cgccttgact tctatagggg cactagatat taatgagcga tttatgctgg aatctatatc
    94861 tttggaattg cttaaacgac agttagtttc gattgataaa atgacttcat tagatggtat
    94921 caaaattggg ttattgaatt caagtgaagc aatcatggtt gttcgagact tcataaaaac
    94981 aaaacaaaat atcccaatca ttttagaccc tgttttggca ttcaaagaaa ctgaagctgt
    95041 gatcaatcaa ttgtatattt cgcagctcat cgaagaactt tttccacttg ttacgatgat
    95101 tacgcctaat ttggcagaag ctgctttact ttctggtcaa gctcttccct cttcgttaac
    95161 agaaatgatc catctcgcta aagaactcac tgcttcagga acaaaaaaca ttgtgatcaa
    95221 aggcggcaca aggatggctg gagatgaagc tgtggatctt ctctattcaa ttgatcatta
    95281 tgaggtattt tctcataaaa aaatcgacac tacaacagta aacggggccg gttgtgcctt
    95341 ttcttctgca ataacagcga atcttgtgtt aggggcagat tttaccaaag cagtagcaga
    95401 aagtaaaaaa tttgttgccc attgtatttc taatggcgtg ctgatgaacg atcataccgg
    95461 aagtgtctgg tctggcggta ttcaaaaagg aggaatgaga aatgaaaact aagcaattaa
    95521 cgatctatgc tatgctgatt gcgttgacag ttgccctttc attaacaatt ttgatccctg
    95581 taccggctac gaatggattt gtgacattat gcgaggccgg gatctatacc gctgcttttc
    95641 tttatggtcc aacgggtgga ttagttgttg gtgcttccag tggtctattg atcgatttga
    95701 tttcaggata tccgcagtgg gcagtttttt cattagtgat ccacgggtta caaggatatg
    95761 ttgctggttc tttcagcaaa tcttctactc gtatgtgggg attagggctt ttattaggaa
    95821 gcttagttat gatcatcggc tatggtatcg ccggttggat tttatatgat tggccggctg
    95881 gagtggcttc tataccagga aacatcttcc aaaatctgat tggaatggga cttgcctttc
    95941 ctataactgc aagtcttcaa cgtattcaag cacggaagca atattaattg aaaaagaagg
    96001 ttttagaaaa tgaagttgtc agaaaaagaa ttaaaagacc aattgactga aatagttaat
    96061 gacatattgg ctgaggcaca cttgaaaaaa ggtgatcttt ttgttttagg atgcagcaca
    96121 agcgaagtcg ttggaggaca tattggaaaa aattccagtg cggaagttgg tcaatggatc
    96181 atccgtacgt taaaagagtt gttggatcct aaagaaattg cgctagctgt ccaaggttgt
    96241 gaacatttga accgagcatt agtagtagaa cgaacagtgg cagaagcaaa aaatttcgaa
    96301 attgtctctg ttgttcctgc actacatgct ggaggtgctt gttctgttgc tgcttttgac
    96361 cagttcaatg atcctgtcga agtggaacac gtagttggac aagccggtat cgatatcggt
    96421 gacacatcaa tcggtatgca tatcaagcat gtccaagtac ctgtcagacc tcgtttgaaa
    96481 acccttggac aagctcacgt gacagccctt cgctctcgtc ctaaatatat cggtggtcct
    96541 agagcgaatt attgaaatga gtaattgaca gagcaatctg aaaaacttcc tcgcaaagtc
    96601 caactaatga actttaacga ggaagtttta cttgtttgtt ttatataacc gagcttcata
    96661 aggtgctagt atgcctttag cttcaaattc tccgctagag gaatttgaca agagtaatgt
    96721 ccaggactga tctgcttctt ttggaagttc atagtcagca agtgattctg ttaagtttac
    96781 cataataaga tattgttcat cttctaaata tctttcatag acataaagtt gtggatgttc
    96841 atgaagatat tctttataag aaccgtatat cagaccttta ttggtttgtc taatgcggat
    96901 catgttctta tagaattgca ggaccgactc tggttcatat tcttgttgtt ctacattgat
    96961 cgttttggta tttggattag tgatcagcca aggttcatgt gtagaaaaac cagcatattg
    97021 ttccgaagtc cattgcatgg gtgtcctgct gttgtctctc gttgtttcac gaatgatatc
    97081 caatgcttct tctcttgtct taccttccgc aagcagcctt ttataaagtc ttttcgaatc
    97141 tactgcatca acttgatcga tcgattcaaa gggcatattc gtcattccga tttcctgtcc
    97201 ttgataaata aatggcgtac cttgcaaaaa gaaatacatc atagcgattg cttttgcaga
    97261 catagcccaa aaatcgggac gagtatctcc aaacactgaa acggcacgag gaacatcatg
    97321 gttttccata tataaagcgt tccatccgat accatccaat gaagtttgcc aagcgcttaa
    97381 tgcatgtttc aatttaagga catctaattg cccttctttt tcttgctgcc agatatggat
    97441 atgatcaaat tcgaatatca tatcgaaata accgtctttc cctacccatt gtggtccttc
    97501 ttctgcagtg acaccacttg cttctccgac agtcatgata tcgtactctt caaaaacatg
    97561 tttcaaatcg gtcaaatgtt cttcgattcc tgagacattt tgaaagggtg agaaagggtt
    97621 ctctgtggct tttactgaat attcatcttt ttttacatga gaaatcgcat ctaaacgaaa
    97681 tccatctatc cctttatcca accaccatcg gatcatattg aatagttctt ctttcaattt
    97741 ctcgctttcc caatttaagt ctggttgttc ttttgcaaaa acatgcagat aatactgtcc
    97801 agtttcatga gaatactccc aagcagaacc gccgaaaatc gatgtccaat tattcggttc
    97861 tttgccatct accccgtcga cccaaatgta ataatcgcga tacgggcttt ctttagattt
    97921 tttcgattct tcaaaccatt gatgctgatc cgaactatgg ttgatgacaa gatccataat
    97981 cactttgatc cctaattcat gtgcttcttt tagcaataaa tcaaaatctt ccatcgttcc
    98041 gaatctctct tctatatctt gatagtcact tacgtcataa ccattatcga caaatggaga
    98101 ttgatagatc ggattgatcc agataaagcc gataccaaga tctttcaaat aattaagttt
    98161 ttcacggatc ccattgatgt ctccttgacc atcgccattg ctatctttga aactagcagg
    98221 atagatttga tagcctactg cttctttcca ccatttttgt tcttgtatca tatcgtttca
    98281 ctcctttaac ggtccctctt gattataacc gattctattt tagaagtatc cttgttaccg
    98341 gtaactagaa acgaaaagct caacgaatct gctaagtgcg ttcgttgagc tttattttat
    98401 catagatagc gggcaatcag ttgaatcatt tgcttgggaa attcattcaa gtcagtaata
    98461 tctacaaaac gactttcacc gtagatcgac ttgatttctt ctttgtcttg gccaatagct
    98521 gcggagacaa ataggattcc ttgacgttca tattctttca aaacagactg gatatcttct
    98581 tttgcttttt tgcctgtata atccggcaat gcttttggct gaccgtcact aatattaagc
    98641 aacagtttgg ttgttgcgga ttgtctgttt aatttttctg ctgctaaacg aagtgcagca
    98701 ccatcacgat tgttttgcct tggtttcata gtgactaatt tttcatttaa ccattgaaat
    98761 ccgtcttcaa attctttata agaaaacaaa gaagtctttt ctcttgacga acggtctgct
    98821 gtatctccgt aaatcaacaa tgggatatcg actttcttag aaaattcggc aatggcaatc
    98881 gctgcttttt ttgccgcttc gatccgatca tcccgtatca ttgacccaga ttcatcgatc
    98941 cgtacagcta ccgctaaaga aggttgctca tgtggcgggt tctttttgtc aaaatttctc
    99001 aaatcaccat atgctacttt gcttgcattg aaccgctgac cttcgtattt tcctttttgg
    99061 tacgtctctg tttgttcatt atccagtaac tctaaaattt gccggctaag tgattctact
    99121 attggcatta cttgcatgtg tagctcacgt gcttccggcg tttttccttg gaaagctggt
    99181 cggtgaacga tgagaccttc tttttcatga atcgtttcct tcatgatttg tcgcgcttct
    99241 ttattcagtt gcttcttgat ttccttatca tgtgcttcca tgtcagaagc agacatttct
    99301 tgagaatcat ctcctgtttg ttgttctttt tgattttttc cttcgatatc gctactgcgt
    99361 tctttttgca tttgaggagg aatctcgttt ttttctattg tttcttcttg tttctcttct
    99421 ccctcatttg gttcttcagc agacaacgat ttcttttcat ctaagcgcag aatttcttca
    99481 gcattttcag tgatgtttcg ttcatttgct tcttcggcgc taatggttct ttcttttgta
    99541 tgctcagatt gaggatgacc ccatttttct tttctcaata tgtcctgaag ttccagtagc
    99601 aatccactgt cagacaaagc ttgttcaaga attgccaatt catctgaatc agtggataat
    99661 ttatacaaaa cttttggata aatcgactgg agtaaatctt gtccttgctt ataggtattt
    99721 gcccaataaa aataagaacg cattcctgct acacctttga tggcgttagc ttttgcagtt
    99781 tcatccaaca aacggataat ctctgccatc tttaagagaa gttcttgttt ttggatcata
    99841 gttttcgcca tcgcacgttc tgccatgact tctaaggcag gaagatccat tttttccgca
    99901 tgttgcatcc gatctcttaa agattcattt aatgggcgat ttccttggta atttcgattt
    99961 gtagtgatca caatgacgca atctggatgg cgacgaatga ttcctgtcgg taaattcaac
   100021 atcccatttg gttcgagcgc tgagttaagt gccacaagaa cagaggcatc tcggatgaca
   100081 gtcggctctt gaatctctaa taaatagcct ttttcaatcg ctcgaacgat ttcagaaggg
   100141 taatagctgt aatggattgc atctggataa tcagcttcga tcctttgaac tagctctgct
   100201 aatttttctt tagcagaaaa aggatcaatg ccgtaatggt tctggatcag tgtcagtaca
   100261 gcttcaagtg tatctgtttg gtaaatagct tctagtaatt cttggtcttg ttccttatta
   100321 gaatctacta caggtaataa ggctccgaaa acatctgatt tgtccatatc tgcaaaacag
   100381 gtcacttttg tatagggaag ttgcagatca gcagatagtg ctttggctaa ttgtgtcttc
   100441 cccgacccgg catctccttc caaaagtacg ttactgattt tcatttcagg atcttgccaa
   100501 ttccctttga tttcggaggc gatccgccat tcttctgcac ttgtccgatg gcttttcggt
   100561 ttcttccaga tcatttgctt ttctagccgt gaaaaggttc ggttttctgt tagtgagaaa
   100621 ttctccatgc ttctcttccc cttttgatca tctctgtcta ttaatatcct agctcatcta
   100681 aaagacgttt taaaatacgg aatcgttccc caatcagttc tccgtctttt tgtagtgtat
   100741 cgtaatacaa aatgatgtct tcatcgatct ttggattttt ggtatctact tctaccgtca
   100801 ttcggttccc ttgtaatcca atgatgtcta cgactggatt ttctggatca taaaattttt
   100861 cataacgata agcatcttgg aagtataaac ttccttgcgc agctaagatc gctaaatcta
   100921 aaatacgtgt gattggtaat tcttccgact gtcgtgacca tttttctcca gtatgtcgcc
   100981 ataccttgcc tgagatatcg acttttccac gatcattcca ctgagcaagg cccagtgaaa
   101041 gcccttttgc atccatattt aatgcatttc ttccatcaac tttatcatag tcttctacaa
   101101 cgattactgg tttatgtttt aaatttgttg gtatttccat tattttaact cctcatcctt
   101161 ttatttttag taaataagta aattagtagt ttactcattt actaaactat atgcgacatt
   101221 atagaggatt gaagtgagga ttgcaaagaa aaagagcttc attgatagaa ataaaaacac
   101281 catctcgttc ttttacacgc tcattagtaa catgtagaaa gtccaagatg atgttggaat
   101341 attt
//
LOCUS       DASHWH010000002        81387 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.002, whole genome shotgun sequence.
ACCESSION   DASHWH010000002 DASHWH010000000
VERSION     DASHWH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.002"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..411
                     /locus_tag="U2R41_000092"
     CDS             <1..411
                     /locus_tag="U2R41_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8887537.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="U2R41_000093"
     CDS             509..697
                     /locus_tag="U2R41_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM8887538.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="U2R41_000094"
     CDS             complement(755..1108)
                     /locus_tag="U2R41_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887539.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="U2R41_000095"
     CDS             1639..2061
                     /locus_tag="U2R41_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8887540.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="U2R41_000096"
     CDS             2058..2288
                     /locus_tag="U2R41_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887541.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="U2R41_000097"
     CDS             2786..2986
                     /locus_tag="U2R41_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM8887542.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="U2R41_000098"
     CDS             3013..4206
                     /locus_tag="U2R41_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8887543.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="U2R41_000099"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="U2R41_000099"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM8887544.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="U2R41_000100"
     CDS             complement(5898..6821)
                     /locus_tag="U2R41_000100"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM8887545.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="U2R41_000101"
     CDS             7017..7622
                     /locus_tag="U2R41_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8887546.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="U2R41_000102"
     CDS             7930..8946
                     /locus_tag="U2R41_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8887547.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="U2R41_000103"
     CDS             9087..10388
                     /locus_tag="U2R41_000103"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM8887548.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="U2R41_000104"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="U2R41_000104"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM8887549.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="U2R41_000105"
     CDS             11117..11515
                     /locus_tag="U2R41_000105"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM8887550.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="U2R41_000106"
     CDS             11581..12663
                     /locus_tag="U2R41_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8887551.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="U2R41_000107"
     CDS             12875..14437
                     /locus_tag="U2R41_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887552.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="U2R41_000108"
     CDS             14430..15563
                     /locus_tag="U2R41_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887553.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="U2R41_000109"
     CDS             15563..16519
                     /locus_tag="U2R41_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887554.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="U2R41_000110"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="U2R41_000110"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM8887555.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="U2R41_000111"
     CDS             17808..18626
                     /locus_tag="U2R41_000111"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8887556.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="U2R41_000112"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="U2R41_000112"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8887557.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="U2R41_000113"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="U2R41_000113"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM8887558.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="U2R41_000114"
     CDS             complement(20506..21087)
                     /locus_tag="U2R41_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8887559.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="U2R41_000115"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="U2R41_000115"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEM8887560.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="U2R41_000116"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="U2R41_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEM8887561.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="U2R41_000117"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="U2R41_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEM8887562.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="U2R41_000118"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="U2R41_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8887563.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="U2R41_000119"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="U2R41_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM8887564.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="U2R41_000120"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="U2R41_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEM8887565.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="U2R41_000121"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="U2R41_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEM8887566.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="U2R41_000122"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="U2R41_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEM8887567.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="U2R41_000123"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="U2R41_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEM8887568.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="U2R41_000124"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="U2R41_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEM8887569.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="U2R41_000125"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="U2R41_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEM8887570.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="U2R41_000126"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="U2R41_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEM8887571.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="U2R41_000127"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="U2R41_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEM8887572.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="U2R41_000128"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="U2R41_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEM8887573.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="U2R41_000129"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="U2R41_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEM8887574.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="U2R41_000130"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="U2R41_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEM8887575.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="U2R41_000131"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="U2R41_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEM8887576.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="U2R41_000132"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="U2R41_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEM8887577.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="U2R41_000133"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="U2R41_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEM8887578.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="U2R41_000134"
     CDS             33541..33726
                     /locus_tag="U2R41_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEM8887579.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="U2R41_000135"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="U2R41_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEM8887580.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="U2R41_000136"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="U2R41_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEM8887581.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="U2R41_000137"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="U2R41_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEM8887582.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="U2R41_000138"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="U2R41_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEM8887583.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="U2R41_000139"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="U2R41_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEM8887584.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="U2R41_000140"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="U2R41_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEM8887585.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="U2R41_000141"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="U2R41_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEM8887586.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="U2R41_000142"
     CDS             37801..38448
                     /locus_tag="U2R41_000142"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEM8887587.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="U2R41_000143"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="U2R41_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEM8887588.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="U2R41_000144"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="U2R41_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEM8887589.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="U2R41_000145"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="U2R41_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEM8887590.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="U2R41_000146"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="U2R41_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEM8887591.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="U2R41_000147"
     CDS             39886..40824
                     /locus_tag="U2R41_000147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEM8887592.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="U2R41_000148"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="U2R41_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEM8887593.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="U2R41_000149"
     CDS             41504..42343
                     /locus_tag="U2R41_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8887594.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="U2R41_000150"
     CDS             42319..43188
                     /locus_tag="U2R41_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8887595.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="U2R41_000151"
     CDS             43178..43975
                     /locus_tag="U2R41_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM8887596.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="U2R41_000152"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="U2R41_000152"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEM8887597.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="U2R41_000153"
     CDS             complement(45257..47926)
                     /locus_tag="U2R41_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEM8887598.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="U2R41_000154"
     CDS             complement(48164..48352)
                     /locus_tag="U2R41_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEM8887599.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="U2R41_000155"
     CDS             48622..48984
                     /locus_tag="U2R41_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8887600.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="U2R41_000156"
     CDS             49036..50718
                     /locus_tag="U2R41_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEM8887601.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="U2R41_000157"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="U2R41_000157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEM8887602.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="U2R41_000158"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="U2R41_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEM8887603.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="U2R41_000159"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="U2R41_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM8887604.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="U2R41_000160"
     CDS             54553..55557
                     /locus_tag="U2R41_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887605.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="U2R41_000161"
     CDS             55558..56502
                     /locus_tag="U2R41_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887606.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="U2R41_000162"
     CDS             56502..57464
                     /locus_tag="U2R41_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887607.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="U2R41_000163"
     CDS             57491..58405
                     /locus_tag="U2R41_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887608.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="U2R41_000164"
     CDS             58431..60212
                     /locus_tag="U2R41_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8887609.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="U2R41_000165"
     CDS             60560..61246
                     /locus_tag="U2R41_000165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEM8887610.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="U2R41_000166"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="U2R41_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEM8887611.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="U2R41_000167"
     CDS             64856..65665
                     /locus_tag="U2R41_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8887612.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="U2R41_000168"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="U2R41_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEM8887613.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="U2R41_000169"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="U2R41_000169"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEM8887614.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="U2R41_000170"
     CDS             complement(69217..69669)
                     /locus_tag="U2R41_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEM8887615.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="U2R41_000171"
     CDS             69819..70769
                     /locus_tag="U2R41_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM8887616.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="U2R41_000172"
     CDS             70762..71721
                     /locus_tag="U2R41_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM8887617.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="U2R41_000173"
     CDS             71718..72473
                     /locus_tag="U2R41_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8887618.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="U2R41_000174"
     CDS             72496..73449
                     /locus_tag="U2R41_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8887619.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74093..74389)
                     /locus_tag="U2R41_000175"
     CDS             complement(74093..74389)
                     /locus_tag="U2R41_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEM8887620.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74744..75100
                     /locus_tag="U2R41_000176"
     CDS             74744..75100
                     /locus_tag="U2R41_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8887621.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75110..76009
                     /locus_tag="U2R41_000177"
     CDS             75110..76009
                     /locus_tag="U2R41_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEM8887622.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            76526..77257
                     /locus_tag="U2R41_000178"
     CDS             76526..77257
                     /locus_tag="U2R41_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8887623.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77402..79429
                     /locus_tag="U2R41_000179"
     CDS             77402..79429
                     /locus_tag="U2R41_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEM8887624.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            79537..80634
                     /locus_tag="U2R41_000180"
     CDS             79537..80634
                     /locus_tag="U2R41_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM8887625.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            80941..>81387
                     /locus_tag="U2R41_000181"
     CDS             80941..>81387
                     /locus_tag="U2R41_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887626.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        26676 a        14593 c        17387 g        22731 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    73921 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    73981 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    74041 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    74101 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    74161 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    74221 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    74281 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    74341 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    74401 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    74461 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    74521 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    74581 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    74641 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    74701 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    74761 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    74821 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    74881 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    74941 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    75001 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    75061 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    75121 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    75181 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    75241 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    75301 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    75361 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    75421 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    75481 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    75541 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    75601 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    75661 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    75721 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    75781 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    75841 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    75901 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    75961 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    76021 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    76081 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    76141 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    76201 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    76261 cagtcaagtc acattttgat agataggagg gggacttacg aattatttta aaggctatga
    76321 cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg
    76381 gctgataatc atttataact aatcgataca taggaccaaa tataggtcta tgttctttta
    76441 tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt
    76501 agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat
    76561 gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa
    76621 ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc
    76681 tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc
    76741 tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa
    76801 aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag
    76861 aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag
    76921 tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct
    76981 gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt
    77041 gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc
    77101 aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga
    77161 tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa
    77221 attagtttgg acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa
    77281 ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc
    77341 taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat
    77401 attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc
    77461 gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga
    77521 aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag
    77581 tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc
    77641 ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa
    77701 tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga
    77761 aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc
    77821 aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa
    77881 tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc
    77941 tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg
    78001 ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag
    78061 tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt
    78121 cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac
    78181 ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc
    78241 ggagcaaaga agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc
    78301 tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg
    78361 tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac
    78421 agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga
    78481 taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat
    78541 gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg
    78601 aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac
    78661 agattatttt attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt
    78721 aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga
    78781 gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta
    78841 ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat
    78901 aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc
    78961 agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa
    79021 aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc
    79081 cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg
    79141 ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt
    79201 tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc
    79261 cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga
    79321 ttctacagct catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac
    79381 ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa
    79441 gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc
    79501 acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg
    79561 agaccaaaac aacaattatt tataacactc ctagttattt taggggcact ttttaacaat
    79621 atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa
    79681 atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta
    79741 aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc
    79801 gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg
    79861 agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct
    79921 aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta
    79981 ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag
    80041 gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag
    80101 acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat
    80161 gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta
    80221 gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa
    80281 gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa
    80341 ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat
    80401 ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt
    80461 gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg
    80521 attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt
    80581 ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa
    80641 gataacaaat agtaaatttt tattattgta ggatggttgg gctcttttta taacaatata
    80701 aaatatacca ttttattaat aaaaatgcat actagtaaat gatataattc taataacagg
    80761 tttgatcgcg agaaaggcca aataaaacgc tctccaaaat gttaaaaaaa agacaggacg
    80821 atgtatatta tacaaacaag aatcgctgac aacagtgact cggatccagg aaatcagttt
    80881 tatccccagc tagttggtga atgcatggaa agtaatatac gtagaaagag ctgatgacta
    80941 atgaattatg agtttttttt aagcaaagaa gatcacagaa gttattcatt acttaaatat
    81001 ttagaagcaa gctcaaattt atcagagtca atatcgaatg ttcaagaaga gttgtcgcta
    81061 tcgacatttc tcttgaaaaa aacgatcgat aagctgaaac atgatttaca aaagttcgat
    81121 ctagagaaaa atttgcgagt aaccgtcagt gaattggata tatgtctgga aattaatgga
    81181 aaatgttcta gcaaagctct gttatctcgt tatataacgg attctttatc tatgaaaatg
    81241 gtattggctt tctttcagga aaaatatatc tcagtagaaa attttgctaa acagcatcac
    81301 gtgagttatt ctgtggctta taaagtatta caaagactaa aaagaaatct aaaaaaatat
    81361 caaatttttt ttgaaaagag aaagttg
//
LOCUS       DASHWH010000003        74211 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.003, whole genome shotgun sequence.
ACCESSION   DASHWH010000003 DASHWH010000000
VERSION     DASHWH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.003"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(105..629)
                     /locus_tag="U2R41_000182"
     CDS             complement(105..629)
                     /locus_tag="U2R41_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8887627.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2R41_000183"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2R41_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEM8887628.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U2R41_000184"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U2R41_000184"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEM8887629.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U2R41_000185"
     CDS             complement(2351..3031)
                     /locus_tag="U2R41_000185"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM8887630.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U2R41_000186"
     CDS             3157..4026
                     /locus_tag="U2R41_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8887631.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U2R41_000187"
     CDS             4052..4567
                     /locus_tag="U2R41_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887632.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U2R41_000188"
     CDS             complement(4614..5207)
                     /locus_tag="U2R41_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8887633.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U2R41_000189"
     CDS             complement(5232..5411)
                     /locus_tag="U2R41_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887634.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U2R41_000190"
     CDS             complement(5488..5997)
                     /locus_tag="U2R41_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887635.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U2R41_000191"
     CDS             complement(6093..6473)
                     /locus_tag="U2R41_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8887636.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6802..7551
                     /locus_tag="U2R41_000192"
     CDS             6802..7551
                     /locus_tag="U2R41_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEM8887637.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7735..9012)
                     /locus_tag="U2R41_000193"
     CDS             complement(7735..9012)
                     /locus_tag="U2R41_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887638.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="U2R41_000194"
     CDS             complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="U2R41_000194"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM8887639.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9934..10878)
                     /locus_tag="U2R41_000195"
     CDS             complement(9934..10878)
                     /locus_tag="U2R41_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM8887640.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10880..11566)
                     /locus_tag="U2R41_000196"
     CDS             complement(10880..11566)
                     /locus_tag="U2R41_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM8887641.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12040..12360
                     /locus_tag="U2R41_000197"
     CDS             12040..12360
                     /locus_tag="U2R41_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM8887642.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12514..13419
                     /locus_tag="U2R41_000198"
     CDS             12514..13419
                     /locus_tag="U2R41_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8887643.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13416..14189
                     /locus_tag="U2R41_000199"
     CDS             13416..14189
                     /locus_tag="U2R41_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8887644.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14370..15794)
                     /locus_tag="U2R41_000200"
     CDS             complement(14370..15794)
                     /locus_tag="U2R41_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8887645.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16005..16451
                     /gene="nrdI"
                     /locus_tag="U2R41_000201"
     CDS             16005..16451
                     /gene="nrdI"
                     /locus_tag="U2R41_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8887646.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16516..18498)
                     /locus_tag="U2R41_000202"
     CDS             complement(16516..18498)
                     /locus_tag="U2R41_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8887647.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="U2R41_000203"
     CDS             complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="U2R41_000203"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM8887648.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19411..20472)
                     /locus_tag="U2R41_000204"
     CDS             complement(19411..20472)
                     /locus_tag="U2R41_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8887649.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20683..21249)
                     /locus_tag="U2R41_000205"
     CDS             complement(20683..21249)
                     /locus_tag="U2R41_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8887650.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="U2R41_000206"
     CDS             complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="U2R41_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM8887651.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23037..24158)
                     /locus_tag="U2R41_000207"
     CDS             complement(23037..24158)
                     /locus_tag="U2R41_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8887652.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="U2R41_000208"
     CDS             24284..25225
                     /locus_tag="U2R41_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM8887653.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="U2R41_000209"
     CDS             complement(25375..26271)
                     /locus_tag="U2R41_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM8887654.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="U2R41_000210"
     CDS             26540..27880
                     /locus_tag="U2R41_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8887655.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="U2R41_000211"
     CDS             28098..28541
                     /locus_tag="U2R41_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM8887656.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="U2R41_000212"
     CDS             complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="U2R41_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM8887657.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30866..32089)
                     /locus_tag="U2R41_000213"
     CDS             complement(30866..32089)
                     /locus_tag="U2R41_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEM8887658.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32120..32959)
                     /locus_tag="U2R41_000214"
     CDS             complement(32120..32959)
                     /locus_tag="U2R41_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM8887659.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32976..33656)
                     /locus_tag="U2R41_000215"
     CDS             complement(32976..33656)
                     /locus_tag="U2R41_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887660.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33658..34731)
                     /locus_tag="U2R41_000216"
     CDS             complement(33658..34731)
                     /locus_tag="U2R41_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8887661.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34787..35025)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35068..35301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35378..36049)
                     /locus_tag="U2R41_000217"
     CDS             complement(35378..36049)
                     /locus_tag="U2R41_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8887662.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="U2R41_000218"
     CDS             complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="U2R41_000218"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEM8887663.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39473..39910
                     /gene="ndk"
                     /locus_tag="U2R41_000219"
     CDS             39473..39910
                     /gene="ndk"
                     /locus_tag="U2R41_000219"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEM8887664.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39932..40798)
                     /locus_tag="U2R41_000220"
     CDS             complement(39932..40798)
                     /locus_tag="U2R41_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8887665.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40958..43051)
                     /locus_tag="U2R41_000221"
     CDS             complement(40958..43051)
                     /locus_tag="U2R41_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8887666.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43800..44423)
                     /locus_tag="U2R41_000222"
     CDS             complement(43800..44423)
                     /locus_tag="U2R41_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887667.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44582..45979)
                     /locus_tag="U2R41_000223"
     CDS             complement(44582..45979)
                     /locus_tag="U2R41_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8887668.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46011..46982)
                     /locus_tag="U2R41_000224"
     CDS             complement(46011..46982)
                     /locus_tag="U2R41_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8887669.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47356..47781
                     /locus_tag="U2R41_000225"
     CDS             47356..47781
                     /locus_tag="U2R41_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887670.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48269..48970)
                     /locus_tag="U2R41_000226"
     CDS             complement(48269..48970)
                     /locus_tag="U2R41_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8887671.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48986..49426)
                     /locus_tag="U2R41_000227"
     CDS             complement(48986..49426)
                     /locus_tag="U2R41_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM8887672.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49426..50001)
                     /locus_tag="U2R41_000228"
     CDS             complement(49426..50001)
                     /locus_tag="U2R41_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEM8887673.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50849..51328)
                     /locus_tag="U2R41_000229"
                     /pseudo
     CDS             complement(50849..51328)
                     /locus_tag="U2R41_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51487..52188)
                     /locus_tag="U2R41_000230"
     CDS             complement(51487..52188)
                     /locus_tag="U2R41_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8887674.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52296..53705)
                     /locus_tag="U2R41_000231"
     CDS             complement(52296..53705)
                     /locus_tag="U2R41_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEM8887675.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53753..55618)
                     /locus_tag="U2R41_000232"
     CDS             complement(53753..55618)
                     /locus_tag="U2R41_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8887676.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55739..56590)
                     /locus_tag="U2R41_000233"
     CDS             complement(55739..56590)
                     /locus_tag="U2R41_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8887677.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56900..57613)
                     /locus_tag="U2R41_000234"
     CDS             complement(56900..57613)
                     /locus_tag="U2R41_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM8887678.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57681..58007)
                     /locus_tag="U2R41_000235"
     CDS             complement(57681..58007)
                     /locus_tag="U2R41_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM8887679.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58130..58357)
                     /locus_tag="U2R41_000236"
     CDS             complement(58130..58357)
                     /locus_tag="U2R41_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM8887680.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58679..59932)
                     /locus_tag="U2R41_000237"
     CDS             complement(58679..59932)
                     /locus_tag="U2R41_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8887681.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59993..60733
                     /locus_tag="U2R41_000238"
     CDS             59993..60733
                     /locus_tag="U2R41_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8887682.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60819..61577)
                     /locus_tag="U2R41_000239"
     CDS             complement(60819..61577)
                     /locus_tag="U2R41_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8887683.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61574..62602)
                     /locus_tag="U2R41_000240"
                     /pseudo
     CDS             complement(61574..62602)
                     /locus_tag="U2R41_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62622..65789)
                     /locus_tag="U2R41_000241"
     CDS             complement(62622..65789)
                     /locus_tag="U2R41_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887684.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66013..66897)
                     /locus_tag="U2R41_000242"
     CDS             complement(66013..66897)
                     /locus_tag="U2R41_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM8887685.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66992..67429)
                     /locus_tag="U2R41_000243"
     CDS             complement(66992..67429)
                     /locus_tag="U2R41_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887686.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67646..68557)
                     /locus_tag="U2R41_000244"
     CDS             complement(67646..68557)
                     /locus_tag="U2R41_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM8887687.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(69014..69442)
                     /locus_tag="U2R41_000245"
     CDS             complement(69014..69442)
                     /locus_tag="U2R41_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887688.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(69470..70108)
                     /locus_tag="U2R41_000246"
     CDS             complement(69470..70108)
                     /locus_tag="U2R41_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887689.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(70112..72094)
                     /locus_tag="U2R41_000247"
     CDS             complement(70112..72094)
                     /locus_tag="U2R41_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEM8887690.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            72274..72939
                     /locus_tag="U2R41_000248"
     CDS             72274..72939
                     /locus_tag="U2R41_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887691.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        21540 a        15441 c        12673 g        24557 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aacagaaagt
     6601 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
     6661 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
     6721 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
     6781 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
     6841 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
     6901 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
     6961 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
     7021 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
     7081 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
     7141 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
     7201 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
     7261 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
     7321 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
     7381 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
     7441 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
     7501 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
     7561 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7621 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     7681 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
     7741 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
     7801 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
     7861 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
     7921 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
     7981 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
     8041 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
     8101 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
     8161 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
     8221 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
     8281 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
     8341 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
     8401 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
     8461 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
     8521 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
     8581 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
     8641 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
     8701 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
     8761 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
     8821 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
     8881 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
     8941 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
     9001 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
     9061 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
     9121 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
     9181 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
     9241 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
     9301 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
     9361 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
     9421 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
     9481 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
     9541 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
     9601 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
     9661 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
     9721 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
     9781 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
     9841 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
     9901 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
     9961 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
    10021 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
    10081 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
    10141 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
    10201 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
    10261 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
    10321 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
    10381 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
    10441 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
    10501 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
    10561 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
    10621 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
    10681 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
    10741 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
    10801 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
    10861 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
    10921 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
    10981 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
    11041 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
    11101 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
    11161 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
    11221 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
    11281 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
    11341 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
    11401 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
    11461 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
    11521 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat
    11581 aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc
    11641 acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa aattatctta
    11701 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
    11761 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
    11821 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
    11881 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacaatctgc
    11941 caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat gattttcctc
    12001 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
    12061 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
    12121 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
    12181 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
    12241 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
    12301 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
    12361 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
    12421 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
    12481 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
    12541 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
    12601 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
    12661 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
    12721 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
    12781 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
    12841 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
    12901 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
    12961 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
    13021 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
    13081 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
    13141 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
    13201 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
    13261 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
    13321 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
    13381 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta aaacaagaat
    13441 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
    13501 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
    13561 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
    13621 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
    13681 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
    13741 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
    13801 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
    13861 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
    13921 atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
    13981 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
    14041 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
    14101 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
    14161 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
    14221 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
    14281 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
    14341 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
    14401 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
    14461 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
    14521 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
    14581 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
    14641 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
    14701 taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac attcgtacat
    14761 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
    14821 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
    14881 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
    14941 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
    15001 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
    15061 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
    15121 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
    15181 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
    15241 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
    15301 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
    15361 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
    15421 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
    15481 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
    15541 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
    15601 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
    15661 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
    15721 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
    15781 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
    15841 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
    15901 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
    15961 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
    16021 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
    16081 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
    16141 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
    16201 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
    16261 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
    16321 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
    16381 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
    16441 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
    16501 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
    16561 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
    16621 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
    16681 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
    16741 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
    16801 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
    16861 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
    16921 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
    16981 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
    17041 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    17101 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
    17161 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
    17221 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
    17281 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
    17341 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
    17401 ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag tttcatcaat
    17461 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
    17521 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
    17581 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
    17641 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
    17701 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
    17761 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
    17821 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
    17881 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
    17941 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
    18001 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
    18061 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
    18121 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    18181 agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc ctaatttcac
    18241 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
    18301 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
    18361 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
    18421 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
    18481 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
    18541 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
    18601 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
    18661 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
    18721 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
    18781 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    18841 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
    18901 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
    18961 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
    19021 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
    19081 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
    19141 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
    19201 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
    19261 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
    19321 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    19381 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    19441 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
    19501 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
    19561 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
    19621 tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga catctcttgc
    19681 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
    19741 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt
    19801 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
    19861 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
    19921 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
    19981 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
    20041 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
    20101 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
    20161 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
    20221 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
    20281 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
    20341 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    20401 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
    20461 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    20521 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
    20581 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
    20641 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
    20701 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
    20761 attgatcact cttatcaagg cattatggca aactaacaga tagacatttt cttcgtcttc
    20821 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
    20881 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
    20941 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
    21001 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
    21061 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
    21121 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
    21181 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
    21241 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
    21301 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
    21361 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
    21421 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
    21481 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
    21541 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
    21601 caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca gatgttttgg
    21661 ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    21721 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    21781 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    21841 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    21901 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    21961 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    22021 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    22081 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    22141 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    22201 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    22261 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    22321 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    22381 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    22441 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    22501 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    22561 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    22621 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    22681 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    22741 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    22801 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    22861 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    22921 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    22981 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    23041 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    23101 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    23161 attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca aatagcgatt
    23221 attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt tccgtggatc
    23281 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    23341 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    23401 aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc taaatccacc
    23461 tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa gaaagctagc
    23521 gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg ttgacctgta
    23581 tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag aacgtttctt
    23641 cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta cccttctaaa
    23701 gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct tcctcctaaa
    23761 gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc tcctaaggca
    23821 taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc taaccccaat
    23881 gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa taaatagctg
    23941 atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt tcccctgagc
    24001 gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac attgattttt
    24061 cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa tgaagaagca
    24121 cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca ctttcttctt
    24181 aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct tccatttttc
    24241 tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc aattagatga
    24541 tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtaga
    24781 agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg ttttttattt
    25381 gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata ggattggctt
    25501 aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag taagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgatggt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct acttctggaa
    28621 cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta gagagacaaa
    28801 tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
    28861 gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt
    28921 ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga aactggcttt
    28981 tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat ccctttttat
    29041 gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc acaaattttt
    29101 ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa agcaaactga
    29161 aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct acgaaacttc
    29221 cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct agcgttctac
    29281 ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca ttaccaggca
    29341 gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt gtcatcaatg
    29401 ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc atcacggttg
    29461 agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata atcaatgaca
    29521 cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt tgttggtttc
    29581 cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag gaagtttgtg
    29641 ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc gttaaaattg
    29701 ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt gaccagattg
    29761 aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa agacttgcgg
    29821 ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat gccgggacag
    29881 ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata gctgctgcta
    29941 gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct gaaacaatag
    30001 ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa aatgcaatcc
    30061 caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt gcacacacaa
    30121 caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg accattagag
    30181 ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa ataaaaataa
    30241 agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg
    30301 ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag atcgtgatat
    30361 taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata accattaata
    30421 caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc tctccttttt
    30481 tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc aattcatccg
    30541 cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa tgactccgcg
    30601 gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt caaatgtatg
    30661 gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac cgactttctg
    30721 aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt accttccata
    30781 agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt atttttataa
    30841 ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt tttcttgcta
    30901 ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa
    30961 gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt
    31021 tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg
    31081 taggctggat tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg
    31141 ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga
    31201 ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa
    31261 actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat
    31321 atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat
    31381 ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt
    31441 ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag
    31501 catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt
    31561 cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc
    31621 ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc
    31681 cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat
    31741 caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc
    31801 cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac
    31861 cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt
    31921 ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat
    31981 agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac
    32041 gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct
    32101 cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc
    32161 cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt
    32221 tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac
    32281 gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat
    32341 ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt
    32401 gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag
    32461 tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa
    32521 gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat
    32581 cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga
    32641 tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga
    32701 aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa
    32761 atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata
    32821 catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat
    32881 ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa
    32941 aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt
    33001 tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc
    33061 gttacatctg tttggaatcg attataccct ctcgatatag caagattccc taatccgcct
    33121 gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag
    33181 acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga
    33241 ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc
    33301 tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc
    33361 gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata
    33421 atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg
    33481 ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc
    33541 atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca
    33601 aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta
    33661 tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat
    33721 ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc
    33781 taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa
    33841 aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg
    33901 atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga
    33961 atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt
    34021 ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct
    34081 tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt
    34141 gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg
    34201 aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc
    34261 attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg
    34321 ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg
    34381 acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt
    34441 tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg
    34501 caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt
    34561 tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc
    34621 gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat
    34681 ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc
    34741 tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt
    34801 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga
    34861 tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga
    34921 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
    34981 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag
    35041 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    35101 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    35161 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    35221 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35281 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactga
    35341 aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt tttttattta
    35401 acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat ttttcctttt
    35461 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35521 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35581 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35641 cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga tccaagatat
    35701 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35761 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35821 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35881 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35941 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    36001 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    36061 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac agaagcaaag
    36121 aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt tcttcacagc
    36181 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36241 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36301 aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga taaatggtac
    36361 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36421 tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact
    36481 tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36541 tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg cattccattt
    36601 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36661 atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa atggcaggaa
    36721 cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36781 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36841 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36901 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36961 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    37021 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    37081 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    37141 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    37201 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag caagataacc
    37261 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37321 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37381 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37441 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37501 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37561 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37621 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37681 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37741 tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga tggcattcaa
    37801 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37861 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37921 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37981 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    38041 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    38101 aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga caagattttc
    38161 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    38221 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38281 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38341 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38401 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38461 gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38521 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38581 cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat atgaaatcgc
    38641 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38701 tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38761 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38821 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38881 agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38941 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    39001 cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    39061 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    39121 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    39181 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39241 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39301 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39361 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39421 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39481 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca
    39541 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39601 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39661 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39721 cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg
    39781 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39841 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39901 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39961 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    40021 attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat
    40081 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgat aagctccgtt
    40141 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    40201 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40261 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40321 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40381 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40441 tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40501 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40561 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40621 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40681 tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt
    40741 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40801 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40861 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40921 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt
    40981 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa
    41041 agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat
    41101 ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca
    41161 agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat
    41221 ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat
    41281 tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa
    41341 aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt
    41401 tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41461 catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca
    41521 tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat
    41581 ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt
    41641 ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat
    41701 gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga
    41761 aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa
    41821 acggctgttg tagatgctca agatattggg cagattctct gaataaatct ttatctaata
    41881 tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat
    41941 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    42001 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt
    42061 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    42121 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga
    42181 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42241 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42301 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42361 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42421 ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42481 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42541 taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga
    42601 taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc
    42661 agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca
    42721 ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42781 taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg
    42841 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg
    42901 ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat
    42961 agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    43021 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    43081 tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt
    43141 tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc
    43201 gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa
    43261 agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc
    43321 cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc
    43381 caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg
    43441 ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    43501 agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg
    43561 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat
    43621 tttttgaaat tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca
    43681 cggtgttcaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    43741 ttttgccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctctgttttt
    43801 cattgtccat aggattgata ttgtccttgt gatgactcat tcgtaataat tgaggcttga
    43861 ttttcttcac ttcccgttat cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc
    43921 gactcgtttt tttgagaaga gacagttgtt ttcttgactt gttcatttgt ctttttccat
    43981 tccgctaaac gatcggcgac cacttttttg tctacttttt cagaataagc ggtgatcact
    44041 tttccatttt tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa
    44101 tgcttgtctt taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta
    44161 ataaattgat tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa
    44221 ttggcagtta ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct
    44281 actgattctt tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt
    44341 tttcctgaaa atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga
    44401 agaaacggtc tttttttcct catgctttcc tccactccta tttaattttg actattctat
    44461 tatatacaaa aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac
    44521 ttctttttca caaaatggta agaatggaga atttcacgca tttgctctca actattcttt
    44581 cttaagcctc aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga
    44641 ttgttctttt tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg
    44701 cattggtcca cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac
    44761 cttcttgaat ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat
    44821 catcgtggat catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt
    44881 tttcagaaat aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat
    44941 agattccttg ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt
    45001 cataattgtc gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt
    45061 ttgcttttac ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt
    45121 tgataaccgt caaatcctct gcatggatca caggcatgag atcaagttct tttactatct
    45181 caatcaattc acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga
    45241 acgcatctgc tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc
    45301 ttggtgtcag attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt
    45361 tgatggctaa cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat
    45421 ctgactcttc tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt
    45481 cattatgagt aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat
    45541 atttttcata ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca
    45601 tcggcatatc gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat
    45661 caaaataacg atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta
    45721 tcaaacggct ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact
    45781 gtacatcctc ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa
    45841 agaacttttg cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc
    45901 ctgaaatcct tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg
    45961 gaaattgata ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt
    46021 ccatgtatga tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt
    46081 cgttttaaca gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg
    46141 attttcggaa ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata
    46201 tccttcccga ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg
    46261 taattgctga caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag
    46321 ccgctttttc tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg
    46381 accgcttctt tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca
    46441 ataaatccga tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt
    46501 tctcgatcat tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg
    46561 ataactggaa tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag
    46621 aagacaatca gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt
    46681 tctggctgat cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt
    46741 tcgatccctg agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca
    46801 atagagaagc ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac
    46861 gctgaaatcg cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag
    46921 taacgggaaa ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc
    46981 atacattctc actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg
    47041 accgcattct ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta
    47101 gagcaacttc ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta
    47161 atatagcatt tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga
    47221 ttttttgcct ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt
    47281 ttttctcttc catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca
    47341 aatgggggaa gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg
    47401 acattgactg gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat
    47461 tcttctgtct taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta
    47521 cgtaaagata tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg
    47581 acacgcacgt atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg
    47641 ctgacagata tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat
    47701 aaaaaagaaa caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat
    47761 ggcaacatct acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt
    47821 tctgctatag taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc
    47881 tgattattgt acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag
    47941 cttgagcata tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc
    48001 ttatcctgtt caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca
    48061 ctatggctga tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt
    48121 aaaacaaaca gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa
    48181 atatttgaat ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt
    48241 ttctttcccg tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt
    48301 tatccgtaaa atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct
    48361 gacggatcac cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca
    48421 cttgtatttt aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg
    48481 tttcataata ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt
    48541 ttttgatacg ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc
    48601 gtttttttgg agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact
    48661 gctgtacttc gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat
    48721 cttcatagat ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta
    48781 aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat
    48841 aaaagagcgg caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct
    48901 cttttgcaag catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa
    48961 tcactgacat acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca
    49021 atgtttcaaa atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt
    49081 gtgtaatcat caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca
    49141 acatagttgc cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt
    49201 caaggatagc aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt
    49261 cctgcgccca tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt
    49321 agaaacttgt gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga
    49381 tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt
    49441 caataataac tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt
    49501 gttggaaaag attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt
    49561 ttctaataat tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt
    49621 gtcgggcgtc agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc
    49681 tagacctcca gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc
    49741 atcgatcgtt tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa
    49801 aagcccattc ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat
    49861 cactcccata atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat
    49921 gtattttcct aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc
    49981 gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct
    50041 acacgtgttt ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata
    50101 gacgccacta tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac
    50161 aaaaataaat aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat
    50221 ttcgtcctat atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt
    50281 aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat
    50341 aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta
    50401 aaaatctttt tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact
    50461 gctggaagaa aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat
    50521 attgggctcg aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata
    50581 ataactttct ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac
    50641 ataaggtata ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt
    50701 caaggagggt gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt
    50761 cgattctttc taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac
    50821 ttttttcttg taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt
    50881 cttcttcctc tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata
    50941 ataaaatggt tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg
    51001 gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc
    51061 aaaacatgct taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg
    51121 ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa
    51181 aagcggtctt caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc
    51241 ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag
    51301 tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg
    51361 taatccagaa acagccgtgt atctgcggta ataagcctaa ccaaacgaaa aatatgagaa
    51421 gctatttttg tttggctgtt cttattactt gatgcagggc agctttttca caacctcttt
    51481 ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt agaccgcctc
    51541 cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt tgatgccgat
    51601 ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc
    51661 atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca aagattcttt
    51721 gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa caaaataaat
    51781 ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct caccgaaaaa
    51841 ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg tttctttccg
    51901 gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg ataatcgatt
    51961 gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga catattcatc
    52021 tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa acagttcttc
    52081 taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga aaatatcttc
    52141 tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa cgtcaccctt
    52201 tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac aaatcacatg
    52261 tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc atttgaggca
    52321 attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt cccatttcct
    52381 tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc tgttgatgca
    52441 ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc gtctgctact
    52501 gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc atcaataatc
    52561 gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat gaataatggt
    52621 ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg atcgatctgc
    52681 cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa gttcccttct
    52741 gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata actaaatccg
    52801 atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg ctctaatttt
    52861 tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta ttctccacgt
    52921 acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt tgctgcctga
    52981 ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca gccgatttgg
    53041 atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac gaattgatta
    53101 tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac taatccttga
    53161 cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca atatttgact
    53221 ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc gatcagtttt
    53281 cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta atgagaaatc
    53341 gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc ataaaattct
    53401 aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt ccaggcaatc
    53461 gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc tttgaaacga
    53521 tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc aatcgtccag
    53581 tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat agcatctgca
    53641 acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg cagcagttgc
    53701 tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta
    53761 caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg
    53821 caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa
    53881 ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga
    53941 ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa
    54001 tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga
    54061 tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc
    54121 ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg
    54181 ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt
    54241 tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga
    54301 atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac
    54361 catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag
    54421 cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa
    54481 tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata
    54541 acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg
    54601 ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata
    54661 catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc
    54721 cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa
    54781 atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc
    54841 ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca
    54901 tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata
    54961 cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga
    55021 tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa
    55081 gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa
    55141 aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat
    55201 aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca
    55261 ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga
    55321 tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt
    55381 cctcatccac gatcgattga taaacatctg ctacattact gccaatcact acttgatact
    55441 gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg
    55501 ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga
    55561 cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg
    55621 ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac
    55681 aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt aacaatcctt
    55741 atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt aaataaatct
    55801 gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg atccgttcga
    55861 cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat tcatcttgct
    55921 cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga gtaatgatac
    55981 gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg atgatactca
    56041 gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg tttccttgtt
    56101 ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt tccacacccg
    56161 tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc ggataaaatt
    56221 tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct cgtgtgatcg
    56281 cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca acttttagtt
    56341 tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc ggaatatcag
    56401 ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat tctttttgga
    56461 ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac
    56521 aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat
    56581 caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg
    56641 tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa
    56701 tcatcatacc atcgaatttt tatttatgca agccttttca tataaaaaat aaaaaaactg
    56761 gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc gtttctcttt
    56821 ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt taatctgtaa
    56881 cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca gatctcatat
    56941 catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca actagtcgcg
    57001 tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc tcgacttcat
    57061 tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg tatgtataca
    57121 cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct agcggtgtaa
    57181 acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct tttcccattt
    57241 cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc aagcctttga
    57301 aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact ggtagatctg
    57361 ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc gcatcaaagt
    57421 caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca ttcgctatag
    57481 catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg aaaatcaaag
    57541 ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt tttgcttttc
    57601 cttttttttt cattttctct ccctccagac tcattttact gtttttcttt tgctctttcc
    57661 aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc tgcgcttttt
    57721 tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc atctagatcc
    57781 aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctaccag ttcttctttg
    57841 attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa aagttctaac
    57901 aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg aaattgtcgc
    57961 caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg cttgccttct
    58021 tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat agatgatgct
    58081 tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat caataattta
    58141 ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt tgtttttcga
    58201 tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat tttccttctt
    58261 cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga tggatacttt
    58321 catcttgttc taactctctt aactgctggg tcagcatttt ttttgtgatc gtagggattt
    58381 gcttgcttct ccacgcctaa aataagcgat attgctcttc cctttatctg gtgtaagcaa
    58441 agcagttagc attttgacga tcgtgggttc cgcttttcac ttcttatcta cttagtatca
    58501 tgaagtatac tttcgtacta atactaagta gcggaagtgt caactacttt gtttaactac
    58561 atagcaaaag aaacagaagg gtgacaacgg tcatcacgct tctgtttctt ttgaatatgc
    58621 tgtgttgttg tctctatctc cgtgtagact ttcctcaaac ttatcctttg acagtaagtc
    58681 agtaggctga tttgctcttt ctagatgcca gatacatttc tacaaaaaaa tagtgattct
    58741 gttttgaaaa ttcgattttt agttctgcat tttcgtaaac atccaacatt tttttcgcac
    58801 tgtatagacc taacccgcca ttttttttag aggtttttcc tcttttgacc atctccgata
    58861 gcgtcaaatt cgttggattt gccttgttcg ctatactaaa acgtgatccc tctttttctt
    58921 ccagcagatt gatcgtaatc ggactctgat cggctacacg atgatcgaat gcgttagaaa
    58981 taaaaataga taaacagcga ataaaaacaa ccagatcaat cgggattgtt ttcacttcga
    59041 caggaataac tacttggatc gggattttct ccgattctgc tttttctaga aattttaaaa
    59101 agacacccct gacagctggg gatacaatat ttgttagctg ttgtatttgc ggtttttcaa
    59161 aaatagctgc agaatgatct tctaatattt ctaaaaattc caacgtttct tttttatttt
    59221 cctgaagagt aatttttaat gaaaacagta gacttttata atcatgtttg aattcctcta
    59281 gatgatggta atattcttca tttatttgat acagttgaag cgattcctga tgttgtcttt
    59341 cttttacttg gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa ctgatgacta
    59401 tcgacaaaag agatagaagc agataattag atgagcggaa gtgaacactt cccaatacca
    59461 agtaaatcac gccccagctt aataaaatac aaatacctcc aaaatagtgt tcagttggat
    59521 aagtaaaaag caagttgaga aatctcgtct gtttgtctat actttttact aatagataga
    59581 aaataaatgc gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc tttcctaaat
    59641 aatccatcag atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa aaaagattac
    59701 tgatcaaaca gctgataaac aggtttttcg tagtaaaata aaaaagaaac atcaataata
    59761 agccaactag gtaaggaaag ctcactttag ggataggaag taaaagtgtc accaccatca
    59821 aagctataca accaaagctg ttttttacta gcagttgttt cgaaaagaat aaatagatac
    59881 ctaaatctaa aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc atcttattcc
    59941 tccattgttt tgtttcaaaa tgtacacata tttttttaat aggagcctgc atatgaaaaa
    60001 gtattttatc atagaagata accagtttca cagcgaatgg atcatcagag aattggtcaa
    60061 tcatcatatc gagaatatcg tgcagctcac ttctatctct caaatcaatc agttgatcca
    60121 gttgggcact attgataatc acgatgtttt ctttatcgat atcgacttga agcaatcact
    60181 cacaggtatt gatgtcgccg aacaaatacg taaaaaaaat gcacatagta atattgtttt
    60241 tttcaccgct tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc ttccgattgg
    60301 atatatcaga aaagacagga atttatctga acaaattcgt caagtgctag aacagatcaa
    60361 ttggaaagaa cagcagatcg taaatgtcga taaaatcttt attgatcaaa aaggtggaag
    60421 tctcgtcctg tatcctcggg aaattttata tattgaaagt atcaaaggtt tgaaaaacca
    60481 agttatggtc aaaacaacta cagaagataa attagtgaat acttctttaa aacaattgaa
    60541 gacagagtta gaaaagtatg cttacttttt acttttaaaa tcttactgta tcaacttatc
    60601 gcaattgcag aaaattgatt ctgctcatta tgtgttagaa tttttcaacg gtgactcctt
    60661 actcgttggt cgaaagatct ttgaaaagac aaaggaacgt ttccatgact tccaaaaaac
    60721 tgcttcgagt tgatgaataa gagctgagac gtaagcatag cagatttcag ggagtgggcc
    60781 ttgaatcggc tccactccct gaaatcgttg atatgatttt acgcttcctc ttctgtagga
    60841 gcatctgtta acgaccatgt gaatgttgaa acgtagttgt ctacagcata attggataca
    60901 ggaacaaata attccactga ttgtctccct tgttccacat tctctcctaa tgaataaatc
    60961 catgtgccaa tcccttcatt tgctcctgca gttactagat ctactgcctg atctgctggg
    61021 atttcgattc cttcagcgcc agttccagaa acggtagatg gcattccact gctattagtt
    61081 gcgctatttc gaatcacggc gtttttgaat ttcacctttg aaccagttag tttattcgta
    61141 tcagatgttg taccaataaa ttctgttcgt ttgactgata atttccaacc ggcataattt
    61201 ccgcttttat ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc agtactgtcc
    61261 gttccgcttt gtaatgcagc atatactgtt tttgctgtac ctaacttgat tgttccaaaa
    61321 tcaagatctg gagcttggtc aatcgtcagt aatccggatg ttccctgatt tggttcgtca
    61381 ggtgtaaaag gagtatcagg atcatttgga tcaacaggag gaactggaac aacgtcttca
    61441 ggatttgcag catctggttt ggtcacagaa atcgttcctt ctgtagtggc tgagctctta
    61501 ttctcagcaa aaacactttg tgtacataat aatagacttc cagctagaac ggtacttaat
    61561 gcaattgttt ttttcatttc tttttccttc tttcttttaa aataaataat ccagcaaata
    61621 agccaataag aatgactcca acaagtatca gccatcgcca gtcaaatgtt tctggctgat
    61681 tttttaaatc aatatccttt tcattcaatt ggtccgctgc atcatcagtt atttcaaaat
    61741 cggtatggag attccagcgt ttttctttcc aagcaccctt cacaaccgct tgataatgtc
    61801 ccggagctaa tttttttcct tctaatggga caagtaaatc aaaatttgta ttgggtgcta
    61861 catctatttt ttcttttttt attgaaaggt aaggctcttt tgtttctttt ttgtagattt
    61921 caaccgatgt agtcaagccg aacatataag aagcatttgg attttgaaga ttcgccttaa
    61981 tcacatttct tccatcgctt tgatccgcat aagctttttt taatttcaaa tctggggaag
    62041 actctttttc gctatttcaa atcaagattg ctttcacttg agaaaactga ttgacgacac
    62101 caactgtact tttgttagaa acgttctctt ttttttcttg aaacgtgatc cctcctgcga
    62161 ccacaccagc cagctctttt tctggcgctt tcagtgaaag tgtctcagtg accgcacttt
    62221 gaggaggaac agttatttct cccactgttt ttgctaagtt tttcatatca taacttaatg
    62281 tttcatcaaa tttacttttt gattctccat attcgatcac accgtttgaa ttcgtcgtag
    62341 cggagtggat caatgtctca actgttactg atttatctgt tcggttaaag accgtgtagg
    62401 ggatttcctg tgttttccca ggctccatcc ttacatttat atatgttttg ttcttatcaa
    62461 tctgattagc cggtaattca aaatctaccg caaaattgaa ttcggaggca tggacaggtg
    62521 tctgccaaag gatgaatcca aggagaagga tcataagtaa agagcagttg tttcgatgac
    62581 ttttattcca tattcgattc atcaatcatc ctccttgatc tttattctgt cggcgcatct
    62641 gctaatgtcc atgttatctc tgctgagtaa ttaccagcta tctgattttc tttggttaag
    62701 acaagtttaa acgcccgacc gttatttaaa tattctgaat aatctgcttg cacattcgtt
    62761 gaattttgtc ctctgacaat ttccagttct tcttcattca ctaattgata ctcctctcct
    62821 ttcttgaaat ataggacatc ctgcatagaa aatcctgcat catttgtgaa aggctgagat
    62881 aattttatcg ttaagatcca cggatttttc gtttctcgct gatcataaat ccataatttt
    62941 tcctgtatat ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt gatctgacca
    63001 aaatcgagtt tggaaggggc aagaaatgag agaccacctt ctactacgat cttcgtagca
    63061 ggtgcaaaag gcggggcatc atgataaaca actggcgtat cacttggatt gtgattccca
    63121 atttcatcgt tggtacttgg cttgttctgc acacccttag cagtagcttc tcctgcttgg
    63181 tcgtttaata caatttctaa tacatctcct gcaaaaagtt gattttctgg gatagaaaga
    63241 acaaattccc ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt ttttttcaat
    63301 gaaaccaagg ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc attgatcaat
    63361 gtggtactta aattagcagg ttcaggagga atcacatcga ctgtcgtttt ggcagagtcc
    63421 agttctgtca cttcttggcc acctcttact tttttagcag caacaacttg cacttgatcc
    63481 tccgttttta atctctcatc attgggtact tgaacttcga acaagccgcc ttgttcgcct
    63541 tctccccatg ggttatatcc tgccccttca ttttcatctg tttgtccttg cgtgagagca
    63601 gtaatttcta cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt cacttctact
    63661 tcgcctgtcc aagcatctct tggctttgcc tcgacacctt cgggaacaac gacatgccca
    63721 tagatttttt tatctgcatt cgttggcact cttaaggatt caatcatcgg gagctgatta
    63781 ttggcactga ttcgattata atttttcatt ccggcacctg tctgagcaaa ttcatttgtt
    63841 aaacctgcac tagggcttga tacgaaattg cctaagtcgg tcccattgat cgataagtct
    63901 gtcatatcag aaaaataatc cgggtctttt tcaaagttat cgaatgttgc gcctgttttt
    63961 cgccataaag cgatttctga atgggcgatc tttaaagaag aatctccatt tccttggaat
    64021 acccagccgc ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa atcaaaatag
    64081 gtaggagatt ctaagtccac attcaacaca tccccactga tgatgccggt cttatcttcc
    64141 cctgttttcc cgaccatgac taaataagtt ttttcacctg ccgtaaaatt taaatcaata
    64201 tctccagttg tatctacagt tgctccggaa tctgcgcgta tatcgatatt tgatttttct
    64261 ccagtgactg taaaatcagc tgaacctgga ctttctagaa aaccagaagc gtcataaaat
    64321 tggatcgctt gatttccttg attgagtctg ttcacgtaag atttgccatc acctgtattt
    64381 cttaccctca atgagcctcc attggttact gtcagctttt gcgtaccatt ctcaaaacga
    64441 actgcgctgg aacgcccact ctgcttatcg atgtcgataa ctgatccggc tccttctacg
    64501 atcaaagaaa ggccttttcc tacaaaacgc atcccattgt tcccgggttg atcgtcatct
    64561 ccagccattt tgaccgagat ttgtccacca cttgatacat ggatttgact agaatctcct
    64621 tgcaagcgaa atccgcccgt agttgctgta ttgatggtga ttttcccacc attcgataca
    64681 gtgactttcg atcctgaacc tctcatttgg atgattccgc gattgttgtc attggtaata
    64741 ttgatctttg tatttaccga aaattcgctt cccgaaccac tgacttcaaa catgacatta
    64801 ccaggtagcg tatcgactgc ctctccttca tcgctaacgc catctgcccg atcataatca
    64861 agagagacct ttgaaccgct taagatatgg attcctggcg ctgtttttcc actaccagtc
    64921 tcatctcctg aatctagaaa accgtagaat ttccctagat acggtccata aagttgacta
    64981 ccattgatca cattcaattg atagcttcta caaaaatagg ctttacccgg agcggcattc
    65041 agtccttcct tcactggttg gacatccaac acaccggaaa caccatctaa tgtgacagtt
    65101 gcacgaggag cgtaaatcag tcctaatttg gacccatcat acaaatcaag tgtcccggta
    65161 aattttattt ctcccgtaaa atgatatctg cttccccaaa aatcggtcat ataagcaatg
    65221 ataatggcat ttccatcgat atttggtgtc aatccacctg gatgtgcagc tgttccttta
    65281 aaattctgga actcgatcga ctgaggtatc tcattgatat aaatatggtg tgtgccgaaa
    65341 ttgtgagcga atccaccaaa atcaataacg atatctcgtt tgatctctaa ttccctgtct
    65401 aaagtcaaag gctgaatcag tcggatctta gttactgatt cgtctgcgag aagctgcgcc
    65461 agttcttctg ttgtggcagc ttcgctgtac ccatccgctg aaacagctct tgccagtgcc
    65521 gcttcactgc tatcagacgt ttgttcagct acttctgagg tctcacttgt cttattttct
    65581 tcttgccttt tattagtaga agcctcactt tgagattctg tagtttcaga agtaatcgat
    65641 tcttctgaaa aagtagtctt gtcaaaactt tgattctctg attgcgttga ttcgtttgtt
    65701 ttctctacat cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa caaaatcgac
    65761 gcaatcagct tttttacaca ttgattcatt ctccaccatc ctctctgacg ttttgacaat
    65821 tttattttat cgttttaatc tatttccaat tgtactttca tatttaaaat atgtattttt
    65881 atggaaaaat ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa ggctgtgaca
    65941 aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc
    66001 acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg
    66061 taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga
    66121 cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt
    66181 taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa
    66241 atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga
    66301 gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc
    66361 tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc
    66421 tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga
    66481 gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt
    66541 gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc
    66601 tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg
    66661 ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact
    66721 atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt
    66781 atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa
    66841 ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac
    66901 tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc
    66961 tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa aacatccata
    67021 ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt
    67081 gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa
    67141 tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa
    67201 atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg
    67261 cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt
    67321 ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt
    67381 atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc
    67441 tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa tatctagggt
    67501 cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtagtaatta
    67561 aactactgtt gatttagttc taataaaaag atttagatga acagttttat tgatttaaat
    67621 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
    67681 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
    67741 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
    67801 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
    67861 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
    67921 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
    67981 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
    68041 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
    68101 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
    68161 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
    68221 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
    68281 caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt tctttggttg
    68341 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
    68401 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
    68461 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
    68521 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
    68581 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
    68641 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
    68701 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
    68761 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
    68821 tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg ttcgattttc
    68881 cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa atgaatatat
    68941 taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct attttatata
    69001 aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat gtgtcagttc
    69061 tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct gatcgaatag
    69121 agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag tggttagaat
    69181 gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca gagctatgat
    69241 taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata atattacctt
    69301 tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca ccgtgaactg
    69361 aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc cctagtgcac
    69421 aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt taaagcatga
    69481 tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata actaacgtac
    69541 cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt ttatccaatg
    69601 acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact ctcgcacagg
    69661 cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc ttcttgttta
    69721 tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca tttaaattcg
    69781 aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc aacccaaaat
    69841 cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc ccagttaatt
    69901 gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca ttcaatccta
    69961 atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc acaagatccc
    70021 cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg
    70081 caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc aataactgac
    70141 ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga
    70201 aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag attacacttg
    70261 tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca aagagcgaat
    70321 aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat atgcataaca
    70381 tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat
    70441 gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac ggaaatgata
    70501 actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt tctttcgaca
    70561 aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata tttctttcca
    70621 aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga
    70681 tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt
    70741 tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat
    70801 gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca
    70861 accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc
    70921 ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc
    70981 cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa cgattggaat
    71041 aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac
    71101 cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag
    71161 ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa
    71221 gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa
    71281 gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc tggaacaaat
    71341 aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga
    71401 atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagt
    71461 cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa
    71521 ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg tctgtatttg
    71581 aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag
    71641 tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat gaaccgttca taggcttctt
    71701 tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa
    71761 tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat
    71821 ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat
    71881 ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga
    71941 cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca
    72001 atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct
    72061 gtttatttat actaaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta
    72121 aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa
    72181 aatcataccg tttttcatca aaaaaacgta tctatcaagt gtataggtca tgtagaataa
    72241 atctatatac tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa aatacgaact
    72301 taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg tgtttccgat
    72361 aaaacgattt ctagttggga aaattctcgt acaatgccag accttgaaat gctgtcactt
    72421 ttacatcata ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca
    72481 atagaaccaa cattgccaga tgaacctctt gttaacaaga aatcttattc atcaaaacaa
    72541 cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt
    72601 tttcttatca attatcaata caaaaatgaa acgatcgatc gattcaatcc ttttttgaaa
    72661 ccacaaatcg gctataccac actccctgta ggatacgtgg acaattccat cgaacgttgg
    72721 gtatgtaatg atatctttgg ccatggtaca tggctgtctt ttcacgctgg agaggtagaa
    72781 aaagagaaaa accatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg
    72841 ctcacttggg aagccattcc ctcagattac caagaaatcc tcagccaaga ctatgacgac
    72901 gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga acgtagagat
    72961 agaaaaaatc aatgcacatg atcttatcca aaaaacaggg gtacaaatta attgaaaaag
    73021 ccttgagatg aaagttccta aataaaaaag ttcagaatga taggtatttc tggcttatag
    73081 tataatattt acgccacagc ctcccaatga ttctttttgg aatcataatc aaggatggac
    73141 attcacaaaa atattttgct cattcgatcc gaatagatta acgaccaatc tagtaagtaa
    73201 tatagattgg tacgtagtca agctgcacat aatttagatc aattttagtt cttttttata
    73261 tttctatgat ctgctcattt acttttatat gatggcttac agtaacataa ataaatatga
    73321 taatcgctgg tagtttatcc aagagtactc aataaaatac aaaatggatt gttcaagcgc
    73381 ctatcataat gataaaatga aacaaataaa cgatacaagg aggaagagac tttgagtaat
    73441 ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt aatctatggg atttcggtag
    73501 catgggcatt tttttataga ggaatcacac caacctggcc gttttatctc ttgttagtgg
    73561 gaatattagt aggaaatttt gcccctcttc actatcgtac gaactttttt ccaactgatt
    73621 ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa cattattgaa atgacaatta
    73681 cctgtataat cattttctta gttatattcc taaacgtctt ctaataagaa tctcttaaaa
    73741 ttagcttgtt acagttagtt acctagttta tataacacag ttatcttatc ttccttatca
    73801 acatcttttg ccaactcttt gtcttaaatt cgaaacatat aagcagagct acgaacacca
    73861 agactgtttc ggctctgttt tttgcatcaa attcactctt atatagctga taatctcggc
    73921 tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa ccttgatgtt
    73981 atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat agatttttaa
    74041 attacacact agtttaaact aaaacaaaca aggaaacact taccatttaa tcataaaatt
    74101 agctcatatg aatatattag aatgtaaaat agactggtca aaatctggtc aaaaaagagg
    74161 actaatacag gtagtcctca gactgtagac aaagtccatt ttatggatga t
//
LOCUS       DASHWH010000004        73262 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.004, whole genome shotgun sequence.
ACCESSION   DASHWH010000004 DASHWH010000000
VERSION     DASHWH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.004"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            508..1071
                     /locus_tag="U2R41_000249"
     CDS             508..1071
                     /locus_tag="U2R41_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887692.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1132..1497)
                     /locus_tag="U2R41_000250"
     CDS             complement(1132..1497)
                     /locus_tag="U2R41_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887693.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1573..1818)
                     /locus_tag="U2R41_000251"
     CDS             complement(1573..1818)
                     /locus_tag="U2R41_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8887694.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1888..2142)
                     /locus_tag="U2R41_000252"
     CDS             complement(1888..2142)
                     /locus_tag="U2R41_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887695.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2306..2629)
                     /locus_tag="U2R41_000253"
     CDS             complement(2306..2629)
                     /locus_tag="U2R41_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887696.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2816..2889
                     /locus_tag="U2R41_000254"
     tRNA            2816..2889
                     /locus_tag="U2R41_000254"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Ile,seq:cat)
     gene            complement(3274..3771)
                     /locus_tag="U2R41_000255"
     CDS             complement(3274..3771)
                     /locus_tag="U2R41_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8887697.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3811..4620)
                     /locus_tag="U2R41_000256"
     CDS             complement(3811..4620)
                     /locus_tag="U2R41_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8887698.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4954..5811
                     /locus_tag="U2R41_000257"
     CDS             4954..5811
                     /locus_tag="U2R41_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8887699.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5804..6706
                     /locus_tag="U2R41_000258"
     CDS             5804..6706
                     /locus_tag="U2R41_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8887700.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6739..7089
                     /locus_tag="U2R41_000259"
     CDS             6739..7089
                     /locus_tag="U2R41_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8887701.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7138..8061
                     /locus_tag="U2R41_000260"
     CDS             7138..8061
                     /locus_tag="U2R41_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8887702.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8139..9032
                     /locus_tag="U2R41_000261"
     CDS             8139..9032
                     /locus_tag="U2R41_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8887703.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9070..9633
                     /gene="amaP"
                     /locus_tag="U2R41_000262"
     CDS             9070..9633
                     /gene="amaP"
                     /locus_tag="U2R41_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM8887704.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9647..9835
                     /locus_tag="U2R41_000263"
     CDS             9647..9835
                     /locus_tag="U2R41_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM8887705.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9848..10750
                     /locus_tag="U2R41_000264"
     CDS             9848..10750
                     /locus_tag="U2R41_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8887706.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10782..11024
                     /locus_tag="U2R41_000265"
     CDS             10782..11024
                     /locus_tag="U2R41_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8887707.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11258..11650)
                     /locus_tag="U2R41_000266"
     CDS             complement(11258..11650)
                     /locus_tag="U2R41_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887708.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIIAFYFISIIRSICGSSGFSKW
                     SYYFVCSPFLSQENLGRHCFIKEASLYQ"
     gene            complement(12286..12513)
                     /locus_tag="U2R41_000267"
     CDS             complement(12286..12513)
                     /locus_tag="U2R41_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8887709.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(12522..12995)
                     /locus_tag="U2R41_000268"
     CDS             complement(12522..12995)
                     /locus_tag="U2R41_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEM8887710.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            complement(13635..13808)
                     /locus_tag="U2R41_000269"
     CDS             complement(13635..13808)
                     /locus_tag="U2R41_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887711.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            14225..14791
                     /gene="amaP"
                     /locus_tag="U2R41_000270"
     CDS             14225..14791
                     /gene="amaP"
                     /locus_tag="U2R41_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM8887712.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            14804..14992
                     /locus_tag="U2R41_000271"
     CDS             14804..14992
                     /locus_tag="U2R41_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM8887713.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            15005..15562
                     /locus_tag="U2R41_000272"
     CDS             15005..15562
                     /locus_tag="U2R41_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8887714.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            15594..15836
                     /locus_tag="U2R41_000273"
     CDS             15594..15836
                     /locus_tag="U2R41_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8887715.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(16137..17216)
                     /locus_tag="U2R41_000274"
     CDS             complement(16137..17216)
                     /locus_tag="U2R41_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8887716.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            17566..18780
                     /locus_tag="U2R41_000275"
     CDS             17566..18780
                     /locus_tag="U2R41_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEM8887717.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            18811..20238
                     /locus_tag="U2R41_000276"
     CDS             18811..20238
                     /locus_tag="U2R41_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8887718.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            20261..21187
                     /gene="glsA"
                     /locus_tag="U2R41_000277"
     CDS             20261..21187
                     /gene="glsA"
                     /locus_tag="U2R41_000277"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEM8887719.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            21395..22855
                     /locus_tag="U2R41_000278"
     CDS             21395..22855
                     /locus_tag="U2R41_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887720.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            23076..24839
                     /locus_tag="U2R41_000279"
     CDS             23076..24839
                     /locus_tag="U2R41_000279"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEM8887721.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            24923..25381
                     /locus_tag="U2R41_000280"
     CDS             24923..25381
                     /locus_tag="U2R41_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEM8887722.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            25490..26173
                     /locus_tag="U2R41_000281"
     CDS             25490..26173
                     /locus_tag="U2R41_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM8887723.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            26320..26934
                     /locus_tag="U2R41_000282"
     CDS             26320..26934
                     /locus_tag="U2R41_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEM8887724.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            26950..27156
                     /locus_tag="U2R41_000283"
     CDS             26950..27156
                     /locus_tag="U2R41_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887725.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            27695..28252
                     /locus_tag="U2R41_000284"
                     /pseudo
     CDS             27695..28252
                     /locus_tag="U2R41_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            28310..30349
                     /locus_tag="U2R41_000285"
     CDS             28310..30349
                     /locus_tag="U2R41_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEM8887726.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(30458..31609)
                     /locus_tag="U2R41_000286"
     CDS             complement(30458..31609)
                     /locus_tag="U2R41_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEM8887727.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(31763..32293)
                     /locus_tag="U2R41_000287"
     CDS             complement(31763..32293)
                     /locus_tag="U2R41_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM8887728.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(32392..32742)
                     /locus_tag="U2R41_000288"
     CDS             complement(32392..32742)
                     /locus_tag="U2R41_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEM8887729.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(32899..34428)
                     /gene="ahpF"
                     /locus_tag="U2R41_000289"
     CDS             complement(32899..34428)
                     /gene="ahpF"
                     /locus_tag="U2R41_000289"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEM8887730.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(34438..35001)
                     /gene="ahpC"
                     /locus_tag="U2R41_000290"
     CDS             complement(34438..35001)
                     /gene="ahpC"
                     /locus_tag="U2R41_000290"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEM8887731.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(35145..35807)
                     /locus_tag="U2R41_000291"
     CDS             complement(35145..35807)
                     /locus_tag="U2R41_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8887732.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            35991..37637
                     /locus_tag="U2R41_000292"
     CDS             35991..37637
                     /locus_tag="U2R41_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEM8887733.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            37628..38710
                     /gene="uxuA"
                     /locus_tag="U2R41_000293"
     CDS             37628..38710
                     /gene="uxuA"
                     /locus_tag="U2R41_000293"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM8887734.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(38818..40371)
                     /locus_tag="U2R41_000294"
                     /pseudo
     CDS             complement(38818..40371)
                     /locus_tag="U2R41_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            40519..41660
                     /locus_tag="U2R41_000295"
                     /pseudo
     CDS             40519..41660
                     /locus_tag="U2R41_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(41786..42865)
                     /locus_tag="U2R41_000296"
     CDS             complement(41786..42865)
                     /locus_tag="U2R41_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8887735.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(42974..43414)
                     /locus_tag="U2R41_000297"
     CDS             complement(42974..43414)
                     /locus_tag="U2R41_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8887736.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(43471..43881)
                     /locus_tag="U2R41_000298"
     CDS             complement(43471..43881)
                     /locus_tag="U2R41_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM8887737.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(44062..44496)
                     /locus_tag="U2R41_000299"
     CDS             complement(44062..44496)
                     /locus_tag="U2R41_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8887738.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(44596..45444)
                     /locus_tag="U2R41_000300"
     CDS             complement(44596..45444)
                     /locus_tag="U2R41_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8887739.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            45833..47599
                     /locus_tag="U2R41_000301"
     CDS             45833..47599
                     /locus_tag="U2R41_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEM8887740.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            47701..48960
                     /locus_tag="U2R41_000302"
     CDS             47701..48960
                     /locus_tag="U2R41_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8887741.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            49107..50408
                     /locus_tag="U2R41_000303"
     CDS             49107..50408
                     /locus_tag="U2R41_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8887742.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            50411..51262
                     /locus_tag="U2R41_000304"
     CDS             50411..51262
                     /locus_tag="U2R41_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8887743.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            51275..52090
                     /locus_tag="U2R41_000305"
     CDS             51275..52090
                     /locus_tag="U2R41_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEM8887744.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            52241..53407
                     /locus_tag="U2R41_000306"
     CDS             52241..53407
                     /locus_tag="U2R41_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8887745.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            53637..54284
                     /locus_tag="U2R41_000307"
     CDS             53637..54284
                     /locus_tag="U2R41_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM8887746.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            54368..55312
                     /locus_tag="U2R41_000308"
     CDS             54368..55312
                     /locus_tag="U2R41_000308"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEM8887747.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            55447..56898
                     /locus_tag="U2R41_000309"
     CDS             55447..56898
                     /locus_tag="U2R41_000309"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEM8887748.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            56919..57755
                     /locus_tag="U2R41_000310"
     CDS             56919..57755
                     /locus_tag="U2R41_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM8887749.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            57755..58414
                     /locus_tag="U2R41_000311"
     CDS             57755..58414
                     /locus_tag="U2R41_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8887750.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            58540..59229
                     /locus_tag="U2R41_000312"
     CDS             58540..59229
                     /locus_tag="U2R41_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887751.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            59235..60191
                     /locus_tag="U2R41_000313"
     CDS             59235..60191
                     /locus_tag="U2R41_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEM8887752.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            60235..60747
                     /locus_tag="U2R41_000314"
     CDS             60235..60747
                     /locus_tag="U2R41_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8887753.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(61003..61551)
                     /locus_tag="U2R41_000315"
     CDS             complement(61003..61551)
                     /locus_tag="U2R41_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8887754.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(61640..62821)
                     /locus_tag="U2R41_000316"
     CDS             complement(61640..62821)
                     /locus_tag="U2R41_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEM8887755.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(63057..64565)
                     /locus_tag="U2R41_000317"
     CDS             complement(63057..64565)
                     /locus_tag="U2R41_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEM8887756.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            64724..64915
                     /locus_tag="U2R41_000318"
     CDS             64724..64915
                     /locus_tag="U2R41_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887757.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            65118..66035
                     /locus_tag="U2R41_000319"
     CDS             65118..66035
                     /locus_tag="U2R41_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEM8887758.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            66028..67101
                     /locus_tag="U2R41_000320"
     CDS             66028..67101
                     /locus_tag="U2R41_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887759.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            67249..67767
                     /locus_tag="U2R41_000321"
     CDS             67249..67767
                     /locus_tag="U2R41_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887760.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            67748..68140
                     /locus_tag="U2R41_000322"
     CDS             67748..68140
                     /locus_tag="U2R41_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEM8887761.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            68216..69151
                     /locus_tag="U2R41_000323"
     CDS             68216..69151
                     /locus_tag="U2R41_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEM8887762.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            69284..70063
                     /locus_tag="U2R41_000324"
     CDS             69284..70063
                     /locus_tag="U2R41_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM8887763.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            70056..70373
                     /locus_tag="U2R41_000325"
     CDS             70056..70373
                     /locus_tag="U2R41_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM8887764.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            70617..72227
                     /locus_tag="U2R41_000326"
     CDS             70617..72227
                     /locus_tag="U2R41_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8887765.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            72384..73232
                     /locus_tag="U2R41_000327"
     CDS             72384..73232
                     /locus_tag="U2R41_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8887766.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        24519 a        12911 c        14244 g        21588 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt
      421 ttttttaata acgtaataat ttttttattt tttgtgcttt cgccgttatt gtagagtgat
      481 ttagcattct tatttttaaa aataactgtg ttatatatta aaaaaggcat aatagttata
      541 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
      601 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      661 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      721 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      781 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      841 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      901 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      961 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
     1021 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
     1081 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
     1141 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
     1201 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
     1261 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
     1321 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
     1381 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
     1441 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
     1501 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1561 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1621 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1681 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1741 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1801 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1861 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1921 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1981 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     2041 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     2101 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     2161 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     2221 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     2281 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     2341 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     2401 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     2461 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     2521 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2581 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2641 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2701 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2761 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc
     2821 tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
     2881 caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     2941 aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga
     3001 ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa
     3061 cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt
     3121 gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc
     3181 aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg
     3241 tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa
     3301 atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc
     3361 aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca
     3421 caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa
     3481 agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac
     3541 tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc
     3601 tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg
     3661 tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa
     3721 ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc
     3781 tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac
     3841 accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc
     3901 attccccatc gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc
     3961 gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga
     4021 atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga
     4081 ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc
     4141 tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc
     4201 aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga
     4261 ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc
     4321 ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag
     4381 aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa
     4441 atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt
     4501 agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc
     4561 tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat
     4621 tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga
     4681 atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta
     4741 agaaaaaggt ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa
     4801 aaacgaatag ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat
     4861 gaaaacaaag catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag
     4921 gttagaaaaa gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct
     4981 gtggtaactg cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca
     5041 ttagaaaatt gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca
     5101 gaatatagta aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc
     5161 caattcctga cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca
     5221 actaccgctt acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa
     5281 gtcctttact tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag
     5341 ccgttaaatg gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa
     5401 agattaggaa tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt
     5461 gatcaatgga ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc
     5521 gatcgaatca tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa
     5581 caacttcatc caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca
     5641 aatgaaaaat atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt
     5701 acaggacatg ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta
     5761 aaaaaattag cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat
     5821 agcagtagtc gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc
     5881 agtattagat aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat
     5941 cgattttgaa agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga
     6001 tgtggaattc ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat
     6061 tccagaaaca gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga
     6121 acgtatctta tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg
     6181 tcttgacttc ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact
     6241 ggaaaaaatg aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat
     6301 caatgtgaaa aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca
     6361 taatccagaa aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg
     6421 ttggttacca cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa
     6481 acatccaaca gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat
     6541 ccatttttca ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata
     6601 ttacgaaaaa tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc
     6661 aaaatatgca aaagttcaac caaaaacaga tgtagttgtt aaataataga agacaacaac
     6721 tgaagagggg gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt
     6781 gaaaaggttg gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt
     6841 ctaagggaca aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg
     6901 gaatttggag aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa
     6961 ttagagcaaa attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc
     7021 ttttcattag aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc
     7081 ttcagctaat agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg
     7141 gatgaagaaa agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat
     7201 agtgcaaagc ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt
     7261 ttcgtaggaa ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa
     7321 ctagagcatc cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa
     7381 aaaaaattaa cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat
     7441 agaaaaaccg aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt
     7501 gaaaagatca tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga
     7561 gagatgaaaa agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa
     7621 gaactgtata aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga
     7681 gatcaacggg aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac
     7741 gatgaacagc tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag
     7801 atctatcgag atttattaga gagtattggt gatctattca acgggatgat ggacaataat
     7861 ctaaatcata tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg
     7921 ttcgcaggga tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc
     7981 tttatactcg tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac
     8041 cggaaagatt atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt
     8101 tttggcaata caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg
     8161 acccgcgaaa cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt
     8221 tacagcagaa tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc
     8281 aactagaaaa ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag
     8341 cgattgcatt tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa
     8401 aagatgcaga ggaagtagca aaatacgtga aagatgcagg acgtaagatt gtcttactac
     8461 cttttgatct tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac
     8521 ttggcgggct agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg
     8581 ctgagttacc acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg
     8641 ctttagtgaa agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt
     8701 ctgttcaagc attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa
     8761 tcgctaactt tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg
     8821 gtgtagcacc tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt
     8881 cgatccctga atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag
     8941 caccagttta tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg
     9001 gagtaactgg tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga
     9061 ggtgcgaaaa tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta
     9121 tctgtgcttt tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag
     9181 aagtttcgat tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc
     9241 gttgcatttg ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca
     9301 atcggaaact ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt
     9361 gaaggaatgg ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat
     9421 gtcaaagcaa cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca
     9481 gcactcattg gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata
     9541 ttgggaacaa aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct
     9601 aaacgaagca atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt
     9661 ttcgacatac aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg
     9721 gatcaccgtg ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc
     9781 tatcggtttt tatctgaaac aaacgaaaat cttagatcac tattttaaat cttaggagga
     9841 aacatccatg aataacaata acaaaaataa taacaataaa atgaacaacc aaaaaccagt
     9901 gtctgcacat ccagacccaa cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa
     9961 caaaacagca ccagtatctg cacatccaga tccagtagtc cctaacgttc aacaaggagt
    10021 agaagagaag aaacatgatg cagaaaagca tgcacctcat catccaggaa ctccagaatc
    10081 taaagttcca gaacaacatg atgcaatcga tccttctaaa cctgtggaag agcaaaaaca
    10141 tccttctgac catgcgaaag cacaagaaaa acttagttct gaccacacaa acggacaagg
    10201 aaaacttggt tctgatcata caaataacca aggaaagcat gattctgaac aggaagattc
    10261 aatgcgcggt gagctgaatt atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat
    10321 tgaacaagta gacggtttat tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa
    10381 acttgtgaat acagataatg taacggctgg gatcgaaaca gaagtcggta aaaaacaagt
    10441 agcagttgat atggatgtca ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga
    10501 aatgcaagaa gtaatcggca aagaagtaaa aaatatgaca catttagaag tgatcgaagt
    10561 caacgctaac gtagtggaca tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt
    10621 gcaagacaaa gtaactgacg cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga
    10681 taaagtaaaa tcagcagctg gaagcggcgc acaaaaagta aaagaaaata cagaaccacg
    10741 ggttcagtaa taaggaagta aaacattagg aggaacgaaa catgggattt atttggtcac
    10801 ttatcgtcgg aggaattatc ggagcaattg caggagcaat cacgaataaa ggttcttcga
    10861 tggggattat tgcaaatgtc gtagcaggtt tagttggatc agcacttggt caagcattat
    10921 taggttcttg gggaccaagt attgcaggaa tggcaatcgt gccatcattg attggtgcca
    10981 tcattttagt actaatcgta tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag
    11041 aggtcgtgaa aaagctgttc ttcatcaagt aataagaaca gccaaacaaa aatagcttct
    11101 catatttttc atttggttgg gcatattatc gaagatgcaa gcttttgaac actgtttata
    11161 cggaataaga ggctgtgaca agacttttgt cacagcctct tatttatgcg ggcaaattcc
    11221 caattagatt tttaaaagtt tttccttaaa tgtgtcttta ttgatacaga gaagcttctt
    11281 taataaaaca atgcctccct aaattttctt gagataggaa aggtgaacat acaaaataat
    11341 acgaccactt tgaaaaccca gatgatccac aaatggatcg tatgatactg atgaagtaaa
    11401 aagcaatgat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
    11461 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
    11521 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
    11581 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
    11641 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
    11701 ttgtttgtga agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata
    11761 atcattatta tcaaaataat gttaaaaata aaaaagaatt gtccatcact gttattcaaa
    11821 tcattttatc aaaactacat agtaaatgtt aaaaagcaac atgacataat atatagaagg
    11881 ctttacagta tatatgggag cttactaaaa aagaatggag taagagttaa cacaatgggc
    11941 cattagctga agttttaagc acttcaaggt atgaaacaga atggcctgta atactacatg
    12001 attatttaga ctgctcaaac tgctatcagt ctggtggaat gaataaagta ttatactaaa
    12061 agcagatcca actacaacgt aacaggaaac cagttgtatc aggaaaagtg atagtacatg
    12121 gagaagaaaa ggcccccttt gtatgagtca gtacatatct tatttataga taaaacggta
    12181 ttcaatcgaa ataaaaaatc tgaataatag acgtaaataa cttctaaaag tctcacacat
    12241 ctatcattca gattattttt tatttactgt ttgttctact ttattttatt cttctggtat
    12301 agtattcgag tcatttttat aggtcaacaa atcacctggt tgacactcca aaacttggca
    12361 gattttttcg agagtggaaa aacggatggc ttttgctttt ccatttttta gaatagagat
    12421 gtttgccatc gtaatgccca ctttctcact caattcagtc acactcattt ttttttgagc
    12481 taatactaca tctaaattaa cgataattgc catgtcgcac ctcacactgt caaatcatga
    12541 tctgtcttaa gtaaaatcgc atgttcaagc agtttttcca gcacgcttgt taatgtgtat
    12601 actgcaaaag gaatgaatac gacagcgatt cctaccaaca tcaaacctgg agcatcgtct
    12661 aaatccgcta ctttcataaa tttaggaagt aagccaaaaa aggcgaaacc aataaacaaa
    12721 atggttcgtt cgattttttt tagtatgttt aagctttgag tggaaaaagc ttggttatct
    12781 tcgatttttt tcaatagttt caataagaaa taagcaataa cgatccctag gagaagggca
    12841 aagtaaatga cagaaacaaa taatgtggtc tgccaatcaa aattgaatcc tttttcgctt
    12901 gtagcgatct gagtataaaa aagtacgccg aacaaaataa atacggcact tatacctaaa
    12961 acgacaaatt ttaatagcaa tgttttaaat ttcattttta tccctctttt atctttgata
    13021 gctaaaatat agcagaataa ttattgtaaa acaactattt tttatcgaaa aacaataaaa
    13081 atatatcgtt tataaaaaat gatagagtat gaaggtgtgg agtgcattta acagcttttt
    13141 ttgattgtaa gaaacattta aaaccagtta ttttagattc tatgtgtgtc tcttctataa
    13201 tgctagttta gatgcctggg attatattgg acaatacagt tggaggattt tttattatct
    13261 agcgaagaga agtgtaagtt aacagaactg tatgagaaaa aagacaacta cgtagtagct
    13321 aaccgattca ttaatttttt caagttcatg tttatttacg tatcagaatt tttcttattc
    13381 aatggtttgg tgtaatcacc acttatttgt cctttattgc ttatacaatg ttatacaaaa
    13441 ggaaaatcaa gcattgacgc atggggtggt actatctagc tgaaatagct taataagcgc
    13501 aagtagatgc attggattag tttataaatc aaaagtgtta gaggtattaa gaagaagtag
    13561 aaaaataaaa tggattgccg aataaaaagt accataaatc ctaaaacatg tgccaacaga
    13621 tagatggcac atgtttattt ttttcgataa ttttgtaaca aaaatacgaa gatgaaaaag
    13681 aatagagatt cccataatgt agtagtccaa ctgaattctt tactgatacc aaataccata
    13741 tttcctagat ttgatacaat caaatagcca atcgttatga tcaatgaata gtacaatctt
    13801 ttcatcatag gacatacctc gtttttattt ataagaatag tataaaccaa agaagtgaat
    13861 aagaaaaatg ataatataaa aataaagtaa gaacgcatta aagtaactaa agttatttat
    13921 tgtatttttt aattctccga aaaaatttaa taaataaata gaatcttgta ataataactg
    13981 agcataatct ttgcaatttt taatcttgaa taatatcctt taagtatagc cagcaatcaa
    14041 aagtaaatag agaataagta agcaattgtt atttcagtat cagactggca ataaaacgcc
    14101 gttatgatcc agcaatgttt ggtagcgctt tatttactga tgctatgcta actgttcgct
    14161 atctatactg tgattgtcgg tagagttatt tacagggtaa aaataagttt ataggaggtg
    14221 gaaaatgaat aaggggttaa aaataatcgg tgttatcatg agtttactgc tactttcagt
    14281 atttctagcg attgcgttga tcaatgcgcc gtatattatg cctgaacaat tggaacgatt
    14341 tcgcttcttt actttaacga attactacat gcaacaatat attttctggg cagctgtagt
    14401 atttgccgtt cttgctatta tcatcttgct agtcgttctt ttctatccaa agtcaagagg
    14461 aactttcgtt atgaaacggg aagatggcaa actaacaatc gacaaaaaag cgatcgaagg
    14521 gttggtacgt tcccatttac acgaagaaga gttcattcat tcacctaaag tacggatacg
    14581 ttcaacgaaa aatcgaatcc atatccatgt caacggagac ttgaaacgaa cttcttcatt
    14641 agtagggaaa acaggtgtat tgatgcaaga tatcgaagaa gaagtaacta aagtactcgg
    14701 aacaaaagaa acggtcaaag tggcagtcac atacagcggt tatgaagaac aagaagatac
    14761 aagagactac aagcactctc gagtggaata ggagggccaa ataatgaaag aactattttc
    14821 agcatacaag ctccctatta tctttggggg tcttggacta ctgctagctg ttttatggtt
    14881 aacgattgga tttttcaaga cattgttgat cataatcatg acagcaattg gcgcaggaat
    14941 cggtttttat ttgaaacagt caggaatagt tgatcaattt tttaaatcat aggaggaaac
    15001 aaagatggaa aacaaaaaca tgaatacaac aaatggatca gttttggcaa atgaaaaagg
    15061 agaaaaccgc atgatgaatg gtgaattgac ttatgaagac aaagtcatcc aaaaaatcat
    15121 cggttatgca ttgaaagatg tagatggtct attgacatta gatggcggat ttttctcaaa
    15181 tatggctgaa aaattagtca acacagataa cgtaacagca ggtatcaata cagaagtagg
    15241 taagaaacaa gtagctgtgg atctagatgt gatcgtagaa tacggtaaag acatcgaaga
    15301 tatttacaac caaatcaaag aattgatcag tactgaagta aacaatatga cacatttaga
    15361 tgttatcgaa gtgaatgtga atgttgcaga catcaaaaca caagaagaat accaaaaaga
    15421 tcaagaaaca gtacaagaca aagtaacaga agcagcaaaa tccacaggac aatttgcttc
    15481 acgccaaaca gataaagcaa aatctgctgt aggcaaaggt gcacaaaaag tcaaagaaaa
    15541 taacgaacca cgtgtacaat aagaagtaag aagtcagtta ggaggaattt aaaatgggat
    15601 ttatttgggc attaatcgtc ggcggtatca ttggagcaat tgcaggagca atcacgaata
    15661 aaggatcatc aatggggatc atcgcaaaca tcattgctgg tttagtaggt tcagcaattg
    15721 gacaagcttt actaggaact tggggaccaa caattgcagg aatggctatc attccatcat
    15781 tgatcggtgc aatcatttta gtattagtcg tttcattctt tgcacgtaaa ttataagcaa
    15841 attgaagaaa aacttatgtt gaaatgaaga acaaatttga gaaccgtagc gacactcgct
    15901 gcggttcttt tttatatatt ttatatgaag tattctttat tccttacgta aatttcaagt
    15961 ttaagttagc cacccatggt gacccggttg ctcgttacca ccaaccgtct aatttttcta
    16021 atcgagtggg cccttgacaa tgagctgagg catggtagtc tcccgactgg ctggccccgg
    16081 ccagctttcc agacacaagc catgccgagg gaggctcgtt gtcaagggcc cacgtgtcaa
    16141 gccgtcaacc gttcctgcct aaatgcctgg acgaacgtgt catgcggcgt tgcatagccc
    16201 aaaatctttc gcgggtaatc gttcatccaa cgctgaatcc gcaagatttg gttttcgctc
    16261 acgttgccga ttgggctgcc cttggggatg aaacggcgaa tcagcttgtg ttggttttcg
    16321 ctcgtcccgc gctcccagga agcatagggg tggctgaagt agatgtttgc cacatcctgc
    16381 aaggcatcgt gcagtcccgc gaactccgag ccgttgtccg aggtaaccgt cttgaacagc
    16441 tggccgaagt acgcaccagc ccgttctctc agctgtcgga ccgcttggtt gaccgattcg
    16501 gcatccttcc cggccagctt cagaatgacc tcgaagcgtg tctgccgttc caccaacgtc
    16561 agcagcacgg catccgactt ggtcttgttt cctacgaccg tgtcgacttc ccaatggccg
    16621 aaggttttcc gggtaccaac cgcctccggg cgctcttcaa tggatttccc tagcacccgt
    16681 ttattcgggc ggttgccatg acgggacgat ttcggtttgc gggacagttt ttctagcagg
    16741 tccatattct tcgttttcat gatgccacgg tcgatccact gatagagggt ggtggtccca
    16801 gggataatgg ccgggtcgaa caattcgtgt tttctggcaa agcccaccac cacgtccggg
    16861 gaccattttt ccaacagcat catgtcgtcc gcccattcaa tgaaggcgtc cgtgtcggcc
    16921 cataagggcc gtctgccgca gttcaagcgg ttcttctcgt aggccgcctg cccggcatcg
    16981 gcgtcgtaga cgtcatgata atagtcgtag accttcccgt tctgattctg gcggttaagc
    17041 tgagtaacgg tcccgcgttt gatttcgttg tttatggtct gcggcacgca gcccaggaca
    17101 tccgcaacct gacggttgga atagttttcc tttttcagga tggcgatttg gcaacgttct
    17161 gagtaggtta ggtgttttcc cttgcgtgaa actgtgttat catggttttg cgtcatgtga
    17221 attcatcctt gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg
    17281 tttttttatt tccaaatggc taagttaatt ataaaatccg cctctttatt ccttatgaaa
    17341 aaatatcttt aaaaataaaa aaagacgaaa ataaacggtt ttctataaaa agaagaccgt
    17401 ttattttttg tctttatttg tgtgaaaaac gaaaaagagc acaacacatc aagcttttat
    17461 taattgtctg tcttttataa atgcaagtat taattcaaaa aaacagaaga aaaaaacaac
    17521 tacaccttct tttatactcg taatcgtaga aaggaggcga aaaaaatgaa tacattacct
    17581 atcaatacag gagatacagc attcatgatt ttatgtacag caatggtttg cctgatgact
    17641 cctggtctag ctttttttta cggcggatta gcacggaaga ataatatact aacgatcatg
    17701 tctcaatctt ttctctccgt tgggataaca acgattatgt gggtatttgg tggattcgga
    17761 ttggcctttg gacgggatat cggcggtgtt attggaaacc cggcagattt ctttttgatg
    17821 agacatgtta caagcttccc caacatgttt catggtgcaa cgattccatt tttaatgttc
    17881 tttctttacc aactgatgtt tatcgtgatt accgttcctt tgatgacagg agcttttgca
    17941 gggagattga atttaaaagg ctatattata cttgttattt tttggaatct gctgatttat
    18001 ttccctgttt gccactggat atggggtggc ggatttttgg atcagatggg atttagagat
    18061 ttcgctggtg gagcagtcat ccacacgaca gctggattcg gctctttagc atgtatcctc
    18121 acacttggtc aaagaaagat tcctttagga gaaagaagtc agcatagcag tcttatggca
    18181 gctgctatcg gtacaggact gctttggttt ggctggttcg gttttaattc cggtggtgca
    18241 cttcgagcag atgaacaagc agtcaatgca tttggttcaa cattcatcgc tcttgcattt
    18301 gcgatgatta cttggctgat cattgcaaaa gtaaaaggaa atggctttga ttttgtcgac
    18361 gtactaacag gatccgttgc tggattggca gccattacgc cttgtgccgg ttatgtagaa
    18421 gcaaaaagtg ccatgctgat tgggatcatt gcgggtatcg tctgtcatgc tgctgttgat
    18481 tttcgtaaaa agaaacaatg ggatgatgca cttgatgtgt ggggtgttca tggcatgggc
    18541 ggattcacag gtacgattct gatagggatc tttgcatcta acagccattt actaacgaat
    18601 ccaagcagct ggtacttctt agggattcaa gtgattggtg tcatcattac tgccgtgtat
    18661 gcctatgtgc tgacaacagt gattttaaaa tcggcgatgc actttacaac gatcactact
    18721 acaaaagaag aacaagaaga aggattggac tttttcttct ttggcaacaa actcaattga
    18781 aacaaattca aatagaaaag aggaagattc atgcaacaaa gcaaaacgaa aattcctgca
    18841 aaaaaattaa cgttctttgg ctttttggct atgaccatct caatggtcgt ttcattatat
    18901 gaatatccca cattcgcgac ctcaggcttc agcttagtat ttttcttgtt attaggcggt
    18961 ttgctatggt ttatccctgt ggcattgtgc gcagcagaga tggcaacggt gaaaggatgg
    19021 gaaaaaggcg gtgtctatac ttgggtcagt cgcacattgg gaaaacgatt cggttttgcc
    19081 gcaatctttt tccaatggtt tgagataaca gttggctact tgaccatgct gtatttctta
    19141 actggtgcat tatcctatgc aacgggtatt ggtgctatac aaaacaataa atatctgaaa
    19201 ttggcaatcc tattgattat tttctgggct attttactct cacagttaaa aggaaccaaa
    19261 tacacttcat tgattgctcg gatcggtttt gtagctggta tcttgttgcc agcattagtc
    19321 ttgtttggct tagggatcca ttatgtcgct tcaggcgctc cactacaaac gatactgtct
    19381 atgaaaacat tgattcctga tttttcgaaa ttgcctacgc tggttgtttt tgtttccttt
    19441 attttagcat atatgggcgt agaaacatct gccagtcatg caaatgaaat ggaaaaccct
    19501 aaaaaggatt atccattagc aatgtttgta ttggttattc tggcaatcat tttagatact
    19561 tttggcggat tgaccgtcgc tactacagta cctcaacatg gtttgtctct taatacaggg
    19621 gtgattcaat cgttagaatt cctattaaga cacattgatc caaatcttgc atggattgcg
    19681 aaaattcttg cgattcttgt ttgtttaggt gtcatcggag aaattgcttc ttgggtaacc
    19741 tctccttcaa aagcacttca cgtagcagca gaagaaggat tacttcctga atattttgca
    19801 aaagaaaaca cacacggtgt tcctgctcat ttgatgatcg caaatgggat cgttgcaaca
    19861 atctgggcag ccgtctttac attaagcggc ggggggaata atctttcatt tcttgcagcg
    19921 atgtcattga ctgttgtaat ctacctgatg ttgtacttta tgtttttcgc gggctatttg
    19981 aagctggttt ttaaagatga cagtcttgaa agagcctatc aggttccagg cggaaaagtt
    20041 gtaaaagtaa tcgttgcttg tttaggtctt ttgacttcac tttttgcttt ttgtatctca
    20101 tttgttccac ctgctagtat cgcagctgga cagcatggga tttatgaaac gattctgatt
    20161 gtttcttatc ttatcgtctt agttattcca tttacgatct acgcatgccg tcataaattc
    20221 cagcatgcag aaaaataaaa aatgaaagct ggttattcaa atggaagtaa aagaattaga
    20281 acaattgatt ggaaaaaatt tccactatta tcaaacagga caagtggcgg attacattcc
    20341 agcattagct gaagtggatc ccaaacaatt aggtatggcg atttatgatt tggatacaaa
    20401 acaactgatt gagggaggag acgccgatgt gcgctttgcc atcgaaagca tgtcaaaagt
    20461 tcccgtattg ttattagcaa tcaaagacaa cggaatagaa aaagtctttc aaacaatcaa
    20521 cacggaacca accggttttg ccttcaattc tatcatgaat atggaaatca atcatcgcaa
    20581 acatccaatg aatccttttg ttaatgcagg agcgattgca acaacctcgt tgattgccgg
    20641 taaagatgca gatgaaaaat ttgagcgaat cttgtcattt atgaaagaga tctgtgatga
    20701 tcctaatatc acattgaatg aagaaatcta tcattctgaa tcaagaacag gtgatatcaa
    20761 tcgctctctt gcttactata tgaaaggtaa tcaaatgatc gaaggagacg taccggagat
    20821 actcgatgct tatttcagac aatgctccct caatgtcacc gctacaggca tagcaaaatt
    20881 agctgctgtg ctggcaaata aaggaattgc tccttggaat ggcaaacaaa tcattactga
    20941 agaaagtgct acaatcgtca aatcaattat gacaactgct ggtttgtatg atgaatcagg
    21001 agagttttcc gtccatgttg gtgtacctgc aaaaagcggt gtaggcggcg ggttgatggc
    21061 agctgtgcca aatcgttacg gcataggtgt cttcagcccg gcactagatc catttggtaa
    21121 cagcgcagct ggtatccaat tgttgaaaga tgtagtcaaa gaactagacg ctgacatctt
    21181 tgaataacca ttcttgaaag aggctggggt aaaagtccaa acggctcaaa gctacctaca
    21241 atgaggctga aattgtgagc ttgtttgact tttgtcccag tttctttttt tattggtttt
    21301 tgaaagggct tttctaaata aaaaggaaac aaaaatacga tttacagata tgattaaata
    21361 aaatcataag aaaaactgga ggcgtgtatc aggaatgctg gaaaattaca tcgagcgaaa
    21421 tatatttaga aaagtgtatc tgtgtgaaca attatttgaa ttccaagaaa tagacatcga
    21481 acaaacagct atttctctaa gggtgactac accaacgatc cttcacgatc tagaaagtct
    21541 agccgagtgt ctagaatatt gtataaaaga acaagtaaga gagaagcata agtataaact
    21601 cgtattcaaa catggtattg ctctttctga gctcacacag tttctgtacg gtcaatctta
    21661 ttttttaaag tttttaagtt attattttaa tggtatattc acgagtacag aactggctga
    21721 tcttgaattt atttctttga gcaaagtata tacaatcaaa aaaatcgtat tggatttttt
    21781 taaagaaaat gattatttga aaaataaaga aatcattatc ccagaatttg actcgcggaa
    21841 catcttgcta gctttagtac gttatgtcaa ttgggaaggc tatgaaaata aaaatccgta
    21901 tgtagaagaa gcttgtaacg agttgattga ttatgtagag actcattttt ttaaaagaag
    21961 atattcggaa gatgaaaagt cctttatcat acgaggaata gagatcgcaa tcggaagaaa
    22021 ggcatttccc cttggactga aagaagaaga taagaaaagc gcagaaacga agccgttatt
    22081 tcaattagtc tgtcaaggat tggaagagca aaaaggaatt ctagacttac aagaagaaga
    22141 tgtctattat attttttctt tgttcaacag tagaaattat accaatgaga acatggagct
    22201 gttaagaaaa gatttcgaag ttgtttataa aaattttgtt ctcaatgacc tatcactgaa
    22261 tgaattgatt ttacagatta ttagtatcgc tgaaatcgac gatgcagaaa attttatttt
    22321 caagcaatcc tttttacaat ttgtccgtac gttgtgggca gatggacaag tatttttacc
    22381 ggaaaggatc tatcttctat cagaaaaaga acaacttctg tacggaaaaa ttgtagaaat
    22441 attggaagag tggaaaaaga aaaatcaaat ccaactaaga tggaatgaaa atttcattcg
    22501 aaaattcgtc aaaagtcttt ctttgatcaa cagtgaacaa gcggggagac aggaaataga
    22561 aatttttgtt gtttcagaaa gttcagtcaa acaatttttt tatcgaaccc aatttcattt
    22621 gtatgcggaa gaaggcatcg ctgaaataaa tcctatttta tttcgttcat tagaagaatt
    22681 gcctgatgaa tgtctatgtg cgaaaaaaag agtggttttg tgcgatgctg ctagctatca
    22741 aaatggatta gaaactgaaa agacaaagat ctatccaatc tcattaaaag gtgcaaacat
    22801 ccatttgtat catattgctc aggaacttca cctattaaaa aaacatattt cttgatgaga
    22861 ccttaattag gagaccttct acaaagcttt ttaattgtct gtagaaggtt tttatttgag
    22921 tcctgattcg tgcgaagatc tacttatata acaagctttc tctgaaattt ttttaaaatg
    22981 ctattgactt agagtcaact ctaagtagta gaatagataa agtgtaacaa atctattttc
    23041 attaaaagaa gatagtacgg aggagagaat gtattatgag tacagtatta catgtattgg
    23101 atgcaatcaa tagccccgca gatgtcaaaa aattatctgt tgaggaaatg aaacagttgg
    23161 cagaagagat gcgccaaaaa atcttgcaaa aggacagcga tgtaggtggg cacgttggtc
    23221 cgaatctagg tgtagtagaa ttaacgattg cgtttcatta tgtatttgat tcaccaaagg
    23281 ataaaatcgt ttgggatgtt tcccatcagt cttatggtca taagctttta actggtcgaa
    23341 aaaatggttt tgaaaaagga cattaccgtg aagtgagcgg atattccaac caacatgaaa
    23401 gtaaacatga ctattttaca gtggggcaca catctacatc tatcagctta gcaacaggtt
    23461 tagctaaagc gagagatctg aagaaagaat caggaaatgt ggtagcattt atcggtgacg
    23521 gctccctaag tggtggctta gcttttgaag gattgaacaa tggtggtgtt ttacaatcca
    23581 atctaatcgt attagtaaac gataatgaga tgtctattga tgagaatcat ggcagtttat
    23641 ataaaaactt agctgagctt cgtcaaacca acggtaaaag cagcaataac ctatttaaag
    23701 caatgggatt tgattatcgt tacgtggata atggaaacga tctggaaacg gtgatccgtt
    23761 tatttgaaga agtaaaagat attgatcatc cgattgtact tcacgtccat acggaaaaag
    23821 gtcgcggtta tcagccggca atcgataata aaatgaaata tcattggcat gtaccatttg
    23881 atctgtccac cggggaggca aagcaagtaa cttctggaga aacatttaat atgatcatca
    23941 tggattattt ggataagaaa atgcaaacaa gtacagctcc tattgccgca gtcaatgcag
    24001 cgataccagg cgcctttgga ctgaaaacat tccaagaaaa acatccagat cattattttg
    24061 acgtggggat cgctgaacag cactctatca ctttcgcttc tgctttggct tctcagggag
    24121 tacgtccagt cgtcttccaa tcaagtactt tcttgcaacg ggcatatgat caattatcac
    24181 atgatttagc gatcaacgag aatccagtga ttattttagt cggtggcgga acgatctcag
    24241 ctagtgaccg aacacaccaa ggaacatttg acattcctta cttatcaagt atcccgaaca
    24301 tcaaatatct tgctccagca acaaaagagg atatgatctc tatgctagat tgggcgttcg
    24361 aacaaacagc gcagcctgta gcaatccgaa ttcctacaca tggtgtagaa catggtacag
    24421 ctatcgcaaa tgattatagt attccagcct atcaaacagt aaataatgga ggaaaagtag
    24481 caattttagc tttaggcggt tttcttgaat taggaaaaga aacagtaaaa gcttttagaa
    24541 aaaaaggaat cgaagcaacg ttgatcaatc caggaacgat cacagaaaat gacgtagaaa
    24601 cattagataa attaaaagaa acacacgaat tagtcatcac attagaagat ggtagtttgt
    24661 caggaggatt tggcgaaaat atcagtcgtt attatggtga ttcttcaatg aacgtattaa
    24721 attttggtgc agagaaagaa ttcacggata gcgttcctgt agggcagctt tatgaacgtt
    24781 atcatttaac accaaaacaa atcgttgaag atagtctgcg tgtcttgaat attcaataat
    24841 tcattatgct caccatccag ataattgttt atactagtat ctggatggtt ttttcattat
    24901 aaaaagcaaa ggggtggatg ttatgtatca gcaagtagaa gaatatgtca aaaagttcca
    24961 agaaaaatat cctagttatc aatcagaaaa attaggtttc ttttcatttg gggatacaga
    25021 aaaaatggca gacgagttga gcgaactagt tgtaaaaaag ataaagactg gtacatcaag
    25081 tggttacgat ctgtataaac aagagaatga gcctttacca aaagttggtc aattagacgt
    25141 ggtcctgaat ggaaaaaatg aaccagtttg tatcatcaaa aatataaatg tgaccatctt
    25201 gccttttagt gaagtcacga aagagcatgc ttggaaagaa ggagaagggg atcggacatt
    25261 agcttattgg cggaaagtcc atgctgagtt ttttaaacat gcttataaag aagcgttgaa
    25321 catagaattt acagaagaga agctggtcgt atacgaagaa tttgaagtaa ttttctttta
    25381 gtaagaaagg tctgctgaaa gaaatagctc attattgtct cctgaataga aaagggttac
    25441 atttttatat gaagctgtat ttttatataa cattactgga ggagatattg tggatacact
    25501 ggaacaaatc aaaggcggag tcgtcgtttc ttgtcaagct ttggaaaacg agccgctaca
    25561 cagttctttt attatgggga gaatggcttt ggctgcaaaa gaaggcggag cagttggtat
    25621 tcgagcaaat acgacagaag atatcaatga aatcaagcga atcgttgatt taccggtaat
    25681 agggattatc aaaagagatt acgatgggtc agaagtgttt attactgcga cagaaaaaga
    25741 agtggaagaa cttttggaga cgcaagcaga aatcattgca ctggatgcaa cttctcgtaa
    25801 acgtccgaca gaaaaaacaa ctgctgaact tgtccaaatg attcacgaac acggtcggtt
    25861 agctatggca gatatttcga cttttgagga agcgcttcaa gcgcaagaag atggttttga
    25921 tcttttgtct acgactttag caggctatac agaatattca cgaaagacgc agacacctga
    25981 ttttgattta ttgaatcaaa tcatagaaca agtcgatatc ccagtaatta tggaaggaca
    26041 tactgactct cctgaacaag tagagaaagc atatgaatta ggtgcttttt cagtcgtagt
    26101 tggatctatt atcacccgtc cgcaattgat cactaaaaga tatgtcgaag ccgcaagtaa
    26161 agcaaataac taaaaaacac gcacggcaca gattcttgac aaaaaagaaa aattgataga
    26221 tagtttaaag cagattctaa gaagatgaaa aaagcattac ttttcatttt gtcttcaaga
    26281 tctgcttttt aacgaatttt atagaaagaa ggcgaaacca tgtttgaacg gagtccttca
    26341 gaattgacag aacgtgaaaa ttacaagttg ctgattggtt cagtcatccc tagaccagta
    26401 gcagtagtaa cgactaaatc agcaaaagat atagtgaata tcgcaccctt cagttacttc
    26461 aatatcgtca gttcgaatcg acccatctta tccttggcga tccagagaaa acaagatgca
    26521 gtaaaagaca cagcaaagaa tattcttgag acaagagaag ctgttatcca tatccttgat
    26581 gaggacaacg tagaagaagt aaataaaaca gcagccaatc tgcctaatga ggaaagcgaa
    26641 ctgtacctga ctgacttgac tttaactgat tcagtaatca tttcagtacc agcattgaca
    26701 gaagcaaaag ttcgttttga aacatcctta tatgaacatg tcgagatacg gaataaaaat
    26761 aaagtaacag ctgatttatt tttgctagaa gtacagaaat atcatttaaa tgaaacagtc
    26821 tatgatgata agacaggaag gatcgatcca agaaaattga atgctgtaag ccgtttggca
    26881 ggaaatgatt atgcagcaat aggagaaatt ttcacgattg cacgtcccga ataaaaagga
    26941 gcactagtta tgttaaaaat aagctataaa ccatcgacag attcaaaaga aatgaaaaaa
    27001 gaatatgaaa cagtgaatga ttttctgcaa gggcagtact tagaagttcc gccattgcaa
    27061 gaccattttg tcgttactac tgttacttta gatggtaagg aaatagaaat gccagatcaa
    27121 acgattagcg gattgtttaa ttactttaac aaataaatat ttattccgaa aagtgaaaaa
    27181 acatataaaa aaacacataa aactaaaaaa ttatcaaaga atcctggtgc aactgaaaga
    27241 atttgcaacg ggattctttc aacttatttg ttattttttt ctaaaaaata ttaaagattg
    27301 ttaaatttca ctatttcatt tcaaattcca ttgcctaatc attaggaaaa gtctatcatg
    27361 aactcgtaaa tagaatatag taataacctt gttaggtgag gctcctatac aaacataggc
    27421 tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg gattaaggct
    27481 ttttccaata gctaagaatg ttttctttac gttgtatagt gctaaaactc ggacgatgag
    27541 atgatgctag gtcagaattc ttgttcgaaa aggtttctga ccttttcttt atatttttgt
    27601 gactgactta tttgatgaga caaactcgct cctcacctga caagtggatt gctgtgtgag
    27661 gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc tcggattcac ttctggttgc
    27721 acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag attcatatgt
    27781 cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg ggggagttga
    27841 tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt atgcaagtat
    27901 cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag tcaagatcga
    27961 tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc aagtgatgga
    28021 tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag acttcgggag
    28081 gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag acgtaacttc
    28141 aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa cgaaccttcc
    28201 agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt agaataaaca
    28261 ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag
    28321 tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc
    28381 agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc
    28441 tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc
    28501 tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt
    28561 agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct
    28621 aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac
    28681 ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca
    28741 aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt
    28801 aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt
    28861 agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg
    28921 cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat
    28981 caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat
    29041 tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata
    29101 ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga
    29161 agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga
    29221 gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc
    29281 agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa
    29341 attgacaatc gatgcgaagg cacaaaaaat agcttttgac agtctaggag gaaaagctgc
    29401 atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag
    29461 ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga
    29521 aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt
    29581 taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt
    29641 gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg
    29701 tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt ccgataatat
    29761 atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa
    29821 attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac aaatttctaa
    29881 tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac agggcgaact
    29941 tctaattact cctatccagc aagcagcaat gtattctgtt tttaccaaca atggcacact
    30001 tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg tcatcagcga
    30061 aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac
    30121 agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat
    30181 caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga
    30241 taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac
    30301 caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt
    30361 ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt
    30421 ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa actttttttg
    30481 taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag
    30541 gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag
    30601 ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac
    30661 cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca
    30721 atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac
    30781 tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt
    30841 cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc
    30901 taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga
    30961 taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt
    31021 tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag
    31081 aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga
    31141 ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta
    31201 cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc
    31261 caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa
    31321 caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca
    31381 aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg
    31441 aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa
    31501 caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc
    31561 caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc
    31621 ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat
    31681 atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt
    31741 ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga
    31801 taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca
    31861 aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga atactactat
    31921 aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga
    31981 tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat
    32041 agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac
    32101 ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat
    32161 aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa
    32221 tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa
    32281 tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta
    32341 ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct
    32401 gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg
    32461 aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt
    32521 aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac
    32581 ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat
    32641 ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat acataggata
    32701 aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt
    32761 ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca
    32821 aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact
    32881 ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt
    32941 agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc
    33001 tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg
    33061 attcatttcg atcgttctat ctagccaatc cgtattaggg agtaagccga ctagaatgaa
    33121 tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga
    33181 ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt
    33241 cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc
    33301 tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt
    33361 accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca
    33421 ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg
    33481 cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat
    33541 attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat
    33601 agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga
    33661 ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc
    33721 gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg
    33781 tccaccgcca atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa
    33841 agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc
    33901 tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa
    33961 catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa
    34021 tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa
    34081 tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc
    34141 actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc
    34201 aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga
    34261 atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc
    34321 tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat
    34381 cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta
    34441 aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc
    34501 cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag
    34561 tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc
    34621 tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa
    34681 aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc
    34741 atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac
    34801 ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg
    34861 acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc
    34921 tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat
    34981 ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt
    35041 gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa
    35101 aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac
    35161 tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc
    35221 gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt
    35281 tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt
    35341 tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg
    35401 aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg
    35461 actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat
    35521 ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt
    35581 tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat cgtatacacg
    35641 agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga
    35701 tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt
    35761 tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca
    35821 aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac
    35881 attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca
    35941 taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt
    36001 taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt
    36061 ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg
    36121 gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg
    36181 aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt
    36241 accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca
    36301 aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact
    36361 ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta
    36421 ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg
    36481 atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt
    36541 ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac
    36601 aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag
    36661 gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat
    36721 ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa
    36781 aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat
    36841 ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc
    36901 cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata
    36961 cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt
    37021 tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt
    37081 ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa
    37141 agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc
    37201 ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta
    37261 aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc
    37321 tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga
    37381 gtccagaccc actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc
    37441 aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg
    37501 gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg
    37561 tagcaggacc aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa
    37621 atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga
    37681 tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt
    37741 aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg
    37801 gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc
    37861 ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg
    37921 aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga
    37981 ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata ttccagatga
    38041 tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg
    38101 ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga
    38161 agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga
    38221 ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc
    38281 tcgaatcgta aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta
    38341 taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg
    38401 gattttagca gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa
    38461 atttttagct aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga
    38521 catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga
    38581 tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc
    38641 attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa
    38701 agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct
    38761 tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta
    38821 ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa
    38881 gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt
    38941 taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac
    39001 gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg
    39061 ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt tcacaccaat
    39121 cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca
    39181 ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc
    39241 catataaaat tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag
    39301 catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt
    39361 atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa
    39421 tacagtacaa agggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt
    39481 ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag
    39541 tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa
    39601 aatatctaca gagagatcaa taaaaaagtc aatatattca tactctgtga attctttttg
    39661 tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa
    39721 tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat
    39781 cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat
    39841 gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt
    39901 tctgccatta ttagaaaagg agatagacaa accgtactat gctaaaaatg cattaatctt
    39961 tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactga aactgataat
    40021 cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg attcgttgca
    40081 gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa aattcaactt
    40141 taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg
    40201 ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat gatctgctaa
    40261 atattgtgta gtaataaaga aacgatgttc agataggaaa agaaaaatat ctaattttgt
    40321 attgcttttt ttcttcaaga taaacgtttt aatcgcttct tgagtttgca tgaaagaggc
    40381 tcctttttac ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac
    40441 aaaaaaacag taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa
    40501 tcaaggagtg agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta
    40561 agcttatcgc ttactataaa tcagacagtc cacacagagg agacagaaac agccaaatgg
    40621 acagaaacca agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc
    40681 aattcaggag tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac
    40741 tattcgggag acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc
    40801 actttgtcga ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt
    40861 accttcaccg ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta
    40921 tcagatggac taccgaacga tcatcattta acgccgtata gtcagcggca gacaattgag
    40981 caagattcag ccatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    41041 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    41101 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa taaatcaaaa
    41161 cctgaaataa cccctggaga aaaaccagat gtaactccag aaccagatac ggattctgga
    41221 aaccaaacag taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa
    41281 ccagaggtag atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga
    41341 gaccaaggaa taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag
    41401 agtaaaaaat caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca
    41461 cgaatatttg atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt
    41521 gctgtcctga aacgaaaaat aaacagctac caaaatccgg agagtcacaa aacaaagtta
    41581 tactgtggag cggaatcatt ctattaagta tagcaacaat gctttctgca aaaagattta
    41641 aacaaaacag atctctctaa catttgaata atgtctgtga acgcattttt caaataaaaa
    41701 gaaatatcca gaaaatgacc ccaaaatacc agattgaaat ctgatatttg gggtcgttca
    41761 tactttatct ttcttcatat ttattttaag ggatatctgt ttcttttgga agatggttcg
    41821 ggatcttttt cactttcaat acatctaaaa agaaggtaaa gatcggaatg ccgacaatca
    41881 agccccaaac gccaaagaac cgttcgctaa caagtaagac cacaaatgta taaaaaatcg
    41941 gcagttctgt tttgctggac atcaacttcg gattcagcac ataagactca attagatgga
    42001 caatcaaaat cgtaatcagg atataaacaa catctcggat gcctccttgg gaataagcta
    42061 tgaaagatag aggaatacaa gaaatgataa cacctgctac tgggatcaga cttaaaatga
    42121 aaatcatgat cgctaaactc agcagctgat gaaagccaaa tacagccaag gcgattgtag
    42181 tgagcaacgt atttaaaaga gcgacaacaa attgagcttc taataccaaa ccaaatgtat
    42241 tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat tcacttttta
    42301 aaaacaatct tgagaataca attgtttttt tcttttcaat cataaaaaag aaacttaaga
    42361 tgaatgacat agcaaacgcc actgccattt ttcctatatc ctgtaaatag ccaagcgcaa
    42421 tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa gtatggatat
    42481 attgtaaaac aggatctgca tcttttggct gatgctcgta aaaatcaata acggactgga
    42541 tcatatcaaa tgtttgatga ataagcatcg ggatatactt tgtgaatgcc agatagataa
    42601 ggtaaacgat cagtgtataa atcatgatag ctaaaacacc tgtagggatt cggacaaatc
    42661 gctgaatgat ttgaacaaga tgaatcatca aataagtaaa gataaatgtg agcaaaatgg
    42721 ttgtaatcat acttctaact aagaaaagaa caaaaacgac aaataataaa acagtgagac
    42781 ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt caaatcgaca acctccaaat
    42841 aacaaaaaat aaacacgtta ttcattatat gatggaatat gaaaataaga aagagaaaga
    42901 gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt
    42961 tcatctagca gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg
    43021 tcagatgttg cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt
    43081 gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg aggagtcagc gtaacattta
    43141 cgattctttc atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt
    43201 ttagtaaggg agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg
    43261 aagtcttttt cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca
    43321 gcggcttgag gagggaagtg tagtgtctaa tgatttcctt agaggcggca tataaaggaa
    43381 aacatacttg attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata
    43441 gtgtatcata gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc
    43501 tatatttcca gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc
    43561 ttgcgtttct tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa
    43621 gctcgtttca ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat
    43681 atttttttgc tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc
    43741 aaacaattgt tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa
    43801 ttcgaatgta cgatcgggat tatgaacttc tccagagcgt ccgcctgtgt tgatcatttt
    43861 cgtagaatac atttttttca taaaattacc acctttaatt atattttata caattgaatt
    43921 ttacacaata taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag
    43981 cacatgaaac agacgcgctt tctcgaatag caaacatata caaaaaaata tcagagacaa
    44041 aaactaatct catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa
    44101 acttacttca cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat
    44161 tgagttagtc acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt
    44221 tcgtaatgct ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt
    44281 atttggatgt tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg
    44341 gaatctcgtg ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc
    44401 ctcaatcagt tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc
    44461 atttaataca aaatctattt ccgttccttt atgcataaag atctcctctc cttttcagtc
    44521 gaacagttac tagattcttt tattatacca gttaaagaaa acgcattcaa aaaaatagcg
    44581 gatttactta ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc
    44641 tacgctgctg acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg
    44701 ggaaaattca tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc
    44761 gccttttgcc tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat
    44821 gtcttccaaa taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc
    44881 gatataagtc atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc
    44941 tattccaaaa acaaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat
    45001 cgaagtattc aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct
    45061 agaagagttg tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat
    45121 gacagacaac aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg
    45181 aaaaaacaag cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc
    45241 tagtaccaac ataacgatat tcaacactaa aataactata gctcggtcaa atcctaaaac
    45301 agcttccagt aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa
    45361 aaacatattg atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca
    45421 tataaattgt ctcgtttctt tcatttatcc acttccttaa ttcattaata atactcttat
    45481 cttattataa cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt
    45541 ctaaattatt atgtttcatt cttctagaat atacttctac gttaaaaact gttaaaaaac
    45601 ttttacttta gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt
    45661 acaggaagta gattaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa
    45721 ataaaaattc ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgt
    45781 ttctctatca cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac
    45841 agctgcaatt tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt
    45901 tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tttgtggaga
    45961 tccatatacc ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct
    46021 aagtaccaat gttcatgatt attgggaaat tgaagtgacg agcgaaacaa ggcgcttaca
    46081 gtatgcgttc catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat
    46141 ttttccttat cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt
    46201 tcatcaaatc gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat
    46261 ttttcccgaa agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg
    46321 gggaagtaaa gatccagacc gagaagactt cttcggggga gatttgcaag gtgtgaggga
    46381 tcatttggat tacttgacgg accttggcgt caatggcatc tatttttgtc ccatatttaa
    46441 agccacatcc aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg
    46501 cgataaaaaa cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat
    46561 gttcgatgct gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa
    46621 aaacggagaa aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc
    46681 ttatcagatg actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt
    46741 tactccattc atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga
    46801 tatcgcaact tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa
    46861 tgaagtcgat catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga
    46921 catctatatc cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt
    46981 ccatgcagtg atgaattatg cattcacaga ttcgattaaa gattattttg caaaaaagaa
    47041 aatcacagct tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca
    47101 ggtgaacgaa ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct
    47161 ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg
    47221 gtctccttgt atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg
    47281 ccgcaaatgt atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa
    47341 agaacttgtt tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg
    47401 gctaatagta tatgatcgtg aagataagct ctatttcaca cgggaactag agggacagat
    47461 catctatgtt tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt
    47521 gattcttagt caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg
    47581 atttttgatc atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa
    47641 gatttgtgcc cattataata agggtgtaac agcacgttga atacacagga ggaaatgaag
    47701 atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc
    47761 ggattagcgg cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa
    47821 acgttaacag tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact
    47881 ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg
    47941 gaatcattag cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat
    48001 gaccggatcg gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa
    48061 gcagcttacg atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa
    48121 cctgcagtaa tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca
    48181 tcaacgttta aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa
    48241 gcaggaaaaa atacaggatt tttagctaaa tggactgact tttattattc ctatggttta
    48301 attgcaggtt acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt
    48361 ttgaataatg caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt
    48421 tggccaaagg gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg
    48481 tccaataaaa ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac
    48541 aatatcaatt atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg
    48601 tttggcggag gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa
    48661 aaatggcttg attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat
    48721 gaaatccctg caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca
    48781 acagctgtga ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc
    48841 gaagtttgga caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca
    48901 aaagaatccg cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga
    48961 ttaaaatgat aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc
    49021 aagagcgaga tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt
    49081 ttagcaagtt tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa
    49141 aaaaaatcac gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt
    49201 ttaaaggtat cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg
    49261 gatttcatgc attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat
    49321 tatttatttt gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt
    49381 acggtttgaa tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa
    49441 tcaatcggac gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat
    49501 tgacacttcc cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat
    49561 tgttcactgc atttactaat tatgacttca atcatattcc accagccagt ttaattgatt
    49621 gggtaggggc taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt
    49681 ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc
    49741 ttggtattct cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg
    49801 gtatcatttt cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga
    49861 atatattcaa cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc
    49921 ttccttttgt agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag
    49981 caattatctt agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa
    50041 tcttacaatc gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat
    50101 ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta
    50161 ttacacagta tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggtg
    50221 gtcccggaag cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata
    50281 aacttacgac tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct
    50341 ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg
    50401 gggattaaca atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt
    50461 ttttacttac ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac
    50521 agcaagtact gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga
    50581 ctggacttta ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa
    50641 aaatacattg atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc
    50701 gggctatact tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt
    50761 agtgattcag atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt
    50821 attagaagcg ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc
    50881 aatgaatact tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc
    50941 tgctaaactt gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt
    51001 caaaccgatg ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat
    51061 gttagctaag ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac
    51121 atttatttct gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat
    51181 tgcactaccg atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac
    51241 atctggcgga acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt
    51301 ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa
    51361 atttttcttg atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta
    51421 tgccaatcag atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga
    51481 tgaacaagga acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc
    51541 gcaacaaacg atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca
    51601 acaatccgat catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa
    51661 ggataaatct ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc
    51721 tgtacgtacg cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg
    51781 tccaacaatc attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag
    51841 tctcatttta ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc
    51901 cattcataca ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat
    51961 ggcagcagca gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac
    52021 aacgataact gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg
    52081 tgtcgaatga caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa
    52141 agtagttttt gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta
    52201 gtattaacgt aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata
    52261 caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac
    52321 aacgaaacaa tacggagata cggacactcc tccccaaatc aaaatcaata tccatgaaga
    52381 aatcccaatg aaagagatta caagtaacgc ttctttggca ctaggagaag cctatatgga
    52441 tcatcgaatc gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc
    52501 aaacagcttt ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac
    52561 gaaacaaaaa aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa
    52621 actttggcta gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac
    52681 gttggaacaa gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga
    52741 aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa
    52801 agagtataat gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac
    52861 caaaagaata aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg
    52921 cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg
    52981 ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac
    53041 agctttgatc catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa
    53101 atatattttc cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa
    53161 aaacgatttg caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga
    53221 acattggaca aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc
    53281 cttctatcga atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa
    53341 tatcgatgtg attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg
    53401 tgcataattg aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga
    53461 tgctccagaa gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt
    53521 tttctttata ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt
    53581 ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc gaatcaatga
    53641 aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa
    53701 ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg
    53761 tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg
    53821 aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag
    53881 ataaataccc agatactgca attactagtt tggaattaga tagtgattgg ggatcttact
    53941 tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa
    54001 atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa
    54061 agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa
    54121 tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag
    54181 gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca
    54241 atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa
    54301 caagagttga tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc
    54361 gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa
    54421 tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc
    54481 aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg
    54541 ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc
    54601 ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt
    54661 atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga
    54721 aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa
    54781 accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat
    54841 cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca
    54901 gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg
    54961 caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt
    55021 cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta
    55081 tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga
    55141 tgcatttgaa catctgctat gtggcgcaag catgttacag attggtacag agcttcataa
    55201 agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa
    55261 aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga
    55321 cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt
    55381 gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag
    55441 gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt
    55501 caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg
    55561 tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa
    55621 aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga
    55681 tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat
    55741 tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag
    55801 tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac
    55861 cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc
    55921 gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc
    55981 ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga
    56041 ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct
    56101 ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag
    56161 tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc
    56221 cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga
    56281 aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg
    56341 tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc
    56401 atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat
    56461 ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca
    56521 attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt
    56581 accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac
    56641 atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc
    56701 ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg
    56761 cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga
    56821 agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta
    56881 ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg
    56941 ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg
    57001 aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca
    57061 cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt
    57121 tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg
    57181 atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta
    57241 tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca
    57301 gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga
    57361 aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta
    57421 aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc
    57481 gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag
    57541 atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat
    57601 tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag
    57661 aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac
    57721 cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcccctaa ttgatttttc
    57781 cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg
    57841 cttcgtgtcg tttcttttag gaaatttggt agggttgctt ttgacagttt taggtctttt
    57901 ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc
    57961 cgcactagtc ctgctgtttc tgttatattt cgggttacct tatcaattaa gtgccttaac
    58021 ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg
    58081 atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt
    58141 ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact
    58201 cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc
    58261 agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta
    58321 tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta
    58381 tttagaaaaa cgttctatcc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct
    58441 ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat
    58501 gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc
    58561 gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa
    58621 gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat
    58681 ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca
    58741 gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa actatatgaa
    58801 gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt
    58861 gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag
    58921 cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta
    58981 agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caaaagatca attgtacaaa
    59041 gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg
    59101 tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt
    59161 cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca
    59221 ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat
    59281 taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag
    59341 tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatccaa
    59401 tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca
    59461 gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc
    59521 tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa
    59581 aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata
    59641 ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa
    59701 cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc
    59761 tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt
    59821 atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt
    59881 acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag
    59941 cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc
    60001 aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc
    60061 ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc
    60121 tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag
    60181 tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat
    60241 attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg
    60301 gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa
    60361 acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt
    60421 gctggaacag ctgcattagt ctcaggcgtg gatcccgttt acacaaaaat agatggtagt
    60481 tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact
    60541 cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa
    60601 aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc
    60661 tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga
    60721 aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt
    60781 aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct
    60841 tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta
    60901 ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg
    60961 tcacgatctc tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt
    61021 ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg
    61081 gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga
    61141 aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat
    61201 cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt
    61261 tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact
    61321 ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat
    61381 ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct
    61441 tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag
    61501 tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc
    61561 tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt
    61621 aactttgctt ttttgtattt tataccagca agccagtcac aatagcactt aaaaagctga
    61681 ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt
    61741 catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa
    61801 cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta
    61861 attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat
    61921 ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta
    61981 ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca
    62041 aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa
    62101 tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat
    62161 cttctttttc ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata
    62221 agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga
    62281 cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag
    62341 ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag
    62401 aagctactcc attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac
    62461 caacagcttt cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag
    62521 aaataaatac aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta
    62581 caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt
    62641 ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa
    62701 caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc
    62761 tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca
    62821 aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa
    62881 tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa
    62941 taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa
    63001 cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat
    63061 tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt
    63121 ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca
    63181 agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat
    63241 aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat
    63301 ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata
    63361 tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat caatgaactt
    63421 gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct
    63481 tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt
    63541 cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct
    63601 tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct
    63661 tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca
    63721 cccatgtcag taggagattg ataaacagat tctttaccag taattttttc aataatacgt
    63781 ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca
    63841 tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa
    63901 gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag
    63961 taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagca agtagcgagc
    64021 tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac
    64081 tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg
    64141 atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc
    64201 gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa gccataacca
    64261 cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg
    64321 atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc
    64381 aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca
    64441 tgcttgtcgt caactaactt cccaccaaat tctaaatata atttgtcata gtggttcact
    64501 cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt
    64561 ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa
    64621 ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat
    64681 agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa
    64741 aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct
    64801 tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac
    64861 tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat
    64921 aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa
    64981 aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta
    65041 taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt
    65101 aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac
    65161 tagaggagac gaacgcggat caggatacag gacaagaatc gtcttctttt tacgatatca
    65221 aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag
    65281 ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag
    65341 aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc
    65401 atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga
    65461 tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa
    65521 gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac
    65581 cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc
    65641 agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt
    65701 tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag
    65761 agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc
    65821 ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc
    65881 gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg
    65941 gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata
    66001 atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa
    66061 aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt
    66121 aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat acatgaaaaa
    66181 gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact
    66241 gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt
    66301 gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat
    66361 tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg
    66421 atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat
    66481 attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc
    66541 aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat
    66601 caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc
    66661 aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc
    66721 tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga
    66781 acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat
    66841 cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg
    66901 aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat
    66961 aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca
    67021 tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca
    67081 ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc
    67141 tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta
    67201 ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat
    67261 aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa
    67321 tttattccga atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc
    67381 ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt
    67441 gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca
    67501 atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt
    67561 ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg
    67621 cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc
    67681 aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga
    67741 ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt
    67801 gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac
    67861 atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt
    67921 ggtcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga
    67981 agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta
    68041 ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa
    68101 tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa
    68161 agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga
    68221 aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt
    68281 tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag
    68341 catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc
    68401 caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag
    68461 tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt
    68521 tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact
    68581 gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga
    68641 attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat
    68701 actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg
    68761 aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat
    68821 cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat
    68881 cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga
    68941 gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc
    69001 agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat
    69061 tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga
    69121 taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat
    69181 gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca
    69241 atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag
    69301 tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt
    69361 aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact
    69421 gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac
    69481 gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt
    69541 attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt
    69601 ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc
    69661 ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg
    69721 tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca
    69781 ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact
    69841 gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa
    69901 tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa
    69961 ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc
    70021 gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt
    70081 tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta
    70141 ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat
    70201 ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga
    70261 atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg
    70321 cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt
    70381 tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca
    70441 tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg
    70501 cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt
    70561 ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga
    70621 ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt
    70681 taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc
    70741 ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta
    70801 cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata
    70861 acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg
    70921 cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta
    70981 acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa
    71041 ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt
    71101 ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc
    71161 gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct
    71221 tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg
    71281 aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg
    71341 tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc
    71401 aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag
    71461 atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga
    71521 tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta agcacgatga
    71581 agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat
    71641 tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt
    71701 tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt
    71761 ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg
    71821 cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag
    71881 gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg
    71941 ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca
    72001 attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt
    72061 tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg
    72121 tggctgtagg aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt
    72181 ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt
    72241 tttttgctgt cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt
    72301 ccaattttag tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc
    72361 tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc
    72421 tgccggaacg tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg
    72481 atgggaaaat ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa
    72541 aaaacagtaa agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat
    72601 atgaggaatt gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga
    72661 tttctggctt tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca
    72721 ttccttatga ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag
    72781 acttaaatga tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag
    72841 ataataccta tgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt
    72901 tgacaaatgg tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt
    72961 ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag
    73021 ctgaagaaat tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga
    73081 tacatgcaaa taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc
    73141 caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa
    73201 aagccatcgg cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa
    73261 at
//
LOCUS       DASHWH010000005        61922 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.005, whole genome shotgun sequence.
ACCESSION   DASHWH010000005 DASHWH010000000
VERSION     DASHWH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.005"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            139..570
                     /gene="mraZ"
                     /locus_tag="U2R41_000328"
     CDS             139..570
                     /gene="mraZ"
                     /locus_tag="U2R41_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEM8887767.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            591..1550
                     /gene="rsmH"
                     /locus_tag="U2R41_000329"
     CDS             591..1550
                     /gene="rsmH"
                     /locus_tag="U2R41_000329"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEM8887768.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1555..1956
                     /gene="ftsL"
                     /locus_tag="U2R41_000330"
     CDS             1555..1956
                     /gene="ftsL"
                     /locus_tag="U2R41_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEM8887769.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1957..4149
                     /locus_tag="U2R41_000331"
     CDS             1957..4149
                     /locus_tag="U2R41_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM8887770.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4175..5137
                     /locus_tag="U2R41_000332"
     CDS             4175..5137
                     /locus_tag="U2R41_000332"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEM8887771.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5151..6512
                     /locus_tag="U2R41_000333"
     CDS             5151..6512
                     /locus_tag="U2R41_000333"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEM8887772.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6538..7626
                     /gene="murG"
                     /locus_tag="U2R41_000334"
     CDS             6538..7626
                     /gene="murG"
                     /locus_tag="U2R41_000334"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEM8887773.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7641..8861
                     /locus_tag="U2R41_000335"
     CDS             7641..8861
                     /locus_tag="U2R41_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEM8887774.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9017..10342
                     /gene="ftsA"
                     /locus_tag="U2R41_000336"
     CDS             9017..10342
                     /gene="ftsA"
                     /locus_tag="U2R41_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEM8887775.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKGDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10365..11606
                     /gene="ftsZ"
                     /locus_tag="U2R41_000337"
     CDS             10365..11606
                     /gene="ftsZ"
                     /locus_tag="U2R41_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEM8887776.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11623..12300
                     /locus_tag="U2R41_000338"
     CDS             11623..12300
                     /locus_tag="U2R41_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEM8887777.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12314..12913
                     /locus_tag="U2R41_000339"
     CDS             12314..12913
                     /locus_tag="U2R41_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEM8887778.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12928..13215
                     /locus_tag="U2R41_000340"
     CDS             12928..13215
                     /locus_tag="U2R41_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEM8887779.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13264..14046
                     /locus_tag="U2R41_000341"
     CDS             13264..14046
                     /locus_tag="U2R41_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEM8887780.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14067..14774
                     /locus_tag="U2R41_000342"
     CDS             14067..14774
                     /locus_tag="U2R41_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEM8887781.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15036..17834
                     /gene="ileS"
                     /locus_tag="U2R41_000343"
     CDS             15036..17834
                     /gene="ileS"
                     /locus_tag="U2R41_000343"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEM8887782.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17883..19409)
                     /locus_tag="U2R41_000344"
     CDS             complement(17883..19409)
                     /locus_tag="U2R41_000344"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEM8887783.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19424..20071)
                     /locus_tag="U2R41_000345"
     CDS             complement(19424..20071)
                     /locus_tag="U2R41_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEM8887784.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20255..20584)
                     /locus_tag="U2R41_000346"
     CDS             complement(20255..20584)
                     /locus_tag="U2R41_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEM8887785.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20761..21489
                     /gene="sfsA"
                     /locus_tag="U2R41_000347"
     CDS             20761..21489
                     /gene="sfsA"
                     /locus_tag="U2R41_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEM8887786.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21505..22518
                     /locus_tag="U2R41_000348"
     CDS             21505..22518
                     /locus_tag="U2R41_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8887787.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22518..23795
                     /locus_tag="U2R41_000349"
     CDS             22518..23795
                     /locus_tag="U2R41_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8887788.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23858..26560
                     /locus_tag="U2R41_000350"
     CDS             23858..26560
                     /locus_tag="U2R41_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8887789.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            26712..27029
                     /locus_tag="U2R41_000351"
     CDS             26712..27029
                     /locus_tag="U2R41_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8887790.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27059..27379
                     /locus_tag="U2R41_000352"
     CDS             27059..27379
                     /locus_tag="U2R41_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8887791.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27487..28947
                     /locus_tag="U2R41_000353"
     CDS             27487..28947
                     /locus_tag="U2R41_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8887792.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29015..29236
                     /locus_tag="U2R41_000354"
     CDS             29015..29236
                     /locus_tag="U2R41_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887793.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29267..29449
                     /locus_tag="U2R41_000355"
     CDS             29267..29449
                     /locus_tag="U2R41_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEM8887794.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29449..29862
                     /locus_tag="U2R41_000356"
     CDS             29449..29862
                     /locus_tag="U2R41_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEM8887795.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            29985..31166
                     /locus_tag="U2R41_000357"
     CDS             29985..31166
                     /locus_tag="U2R41_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8887796.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31237..31401)
                     /locus_tag="U2R41_000358"
     CDS             complement(31237..31401)
                     /locus_tag="U2R41_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887797.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31555..31628
                     /locus_tag="U2R41_000359"
     tRNA            31555..31628
                     /locus_tag="U2R41_000359"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31589..31591,aa:Arg,seq:cct)
     gene            complement(31697..32836)
                     /locus_tag="U2R41_000360"
     CDS             complement(31697..32836)
                     /locus_tag="U2R41_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8887798.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(33135..33770)
                     /locus_tag="U2R41_000361"
     CDS             complement(33135..33770)
                     /locus_tag="U2R41_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887799.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(33883..34518)
                     /locus_tag="U2R41_000362"
     CDS             complement(33883..34518)
                     /locus_tag="U2R41_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEM8887800.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(34552..35013)
                     /locus_tag="U2R41_000363"
     CDS             complement(34552..35013)
                     /locus_tag="U2R41_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEM8887801.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(35143..35574)
                     /locus_tag="U2R41_000364"
     CDS             complement(35143..35574)
                     /locus_tag="U2R41_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM8887802.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(35592..35912)
                     /locus_tag="U2R41_000365"
     CDS             complement(35592..35912)
                     /locus_tag="U2R41_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8887803.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            36211..36987
                     /locus_tag="U2R41_000366"
     CDS             36211..36987
                     /locus_tag="U2R41_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEM8887804.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            37002..37205
                     /locus_tag="U2R41_000367"
     CDS             37002..37205
                     /locus_tag="U2R41_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887805.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            37221..37559
                     /locus_tag="U2R41_000368"
     CDS             37221..37559
                     /locus_tag="U2R41_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEM8887806.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            37546..37725
                     /locus_tag="U2R41_000369"
     CDS             37546..37725
                     /locus_tag="U2R41_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887807.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(37768..38238)
                     /locus_tag="U2R41_000370"
     CDS             complement(37768..38238)
                     /locus_tag="U2R41_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887808.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            38325..39023
                     /locus_tag="U2R41_000371"
     CDS             38325..39023
                     /locus_tag="U2R41_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEM8887809.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            39201..39542
                     /locus_tag="U2R41_000372"
     CDS             39201..39542
                     /locus_tag="U2R41_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887810.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            39535..40206
                     /locus_tag="U2R41_000373"
     CDS             39535..40206
                     /locus_tag="U2R41_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEM8887811.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            40212..40898
                     /locus_tag="U2R41_000374"
     CDS             40212..40898
                     /locus_tag="U2R41_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887812.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            40901..41650
                     /locus_tag="U2R41_000375"
     CDS             40901..41650
                     /locus_tag="U2R41_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887813.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            41662..41931
                     /locus_tag="U2R41_000376"
     CDS             41662..41931
                     /locus_tag="U2R41_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887814.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            41937..42101
                     /locus_tag="U2R41_000377"
     CDS             41937..42101
                     /locus_tag="U2R41_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887815.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            42094..42393
                     /locus_tag="U2R41_000378"
     CDS             42094..42393
                     /locus_tag="U2R41_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887816.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            42393..42695
                     /locus_tag="U2R41_000379"
     CDS             42393..42695
                     /locus_tag="U2R41_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEM8887817.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            42735..43100
                     /locus_tag="U2R41_000380"
     CDS             42735..43100
                     /locus_tag="U2R41_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887818.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            43590..43730
                     /locus_tag="U2R41_000381"
     CDS             43590..43730
                     /locus_tag="U2R41_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887819.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            43727..43915
                     /locus_tag="U2R41_000382"
     CDS             43727..43915
                     /locus_tag="U2R41_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887820.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            43923..44117
                     /locus_tag="U2R41_000383"
     CDS             43923..44117
                     /locus_tag="U2R41_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887821.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(44150..44362)
                     /locus_tag="U2R41_000384"
     CDS             complement(44150..44362)
                     /locus_tag="U2R41_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887822.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            44487..44690
                     /locus_tag="U2R41_000385"
     CDS             44487..44690
                     /locus_tag="U2R41_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887823.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            44687..44959
                     /locus_tag="U2R41_000386"
     CDS             44687..44959
                     /locus_tag="U2R41_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEM8887824.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            44960..45145
                     /locus_tag="U2R41_000387"
     CDS             44960..45145
                     /locus_tag="U2R41_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887825.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            45132..45479
                     /locus_tag="U2R41_000388"
     CDS             45132..45479
                     /locus_tag="U2R41_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887826.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            45439..45915
                     /locus_tag="U2R41_000389"
     CDS             45439..45915
                     /locus_tag="U2R41_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEM8887827.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(46061..46789)
                     /locus_tag="U2R41_000390"
     CDS             complement(46061..46789)
                     /locus_tag="U2R41_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEM8887828.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            46837..47115
                     /locus_tag="U2R41_000391"
     CDS             46837..47115
                     /locus_tag="U2R41_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887829.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            47117..47503
                     /locus_tag="U2R41_000392"
     CDS             47117..47503
                     /locus_tag="U2R41_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887830.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            47500..47880
                     /locus_tag="U2R41_000393"
     CDS             47500..47880
                     /locus_tag="U2R41_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM8887831.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            47992..48444
                     /locus_tag="U2R41_000394"
     CDS             47992..48444
                     /locus_tag="U2R41_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEM8887832.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            48441..50168
                     /locus_tag="U2R41_000395"
     CDS             48441..50168
                     /locus_tag="U2R41_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM8887833.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            50232..51419
                     /locus_tag="U2R41_000396"
     CDS             50232..51419
                     /locus_tag="U2R41_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8887834.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            51391..51960
                     /locus_tag="U2R41_000397"
     CDS             51391..51960
                     /locus_tag="U2R41_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEM8887835.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            51973..53337
                     /locus_tag="U2R41_000398"
     CDS             51973..53337
                     /locus_tag="U2R41_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM8887836.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            53339..53620
                     /locus_tag="U2R41_000399"
     CDS             53339..53620
                     /locus_tag="U2R41_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEM8887837.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            53598..53924
                     /locus_tag="U2R41_000400"
     CDS             53598..53924
                     /locus_tag="U2R41_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM8887838.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            53914..54252
                     /locus_tag="U2R41_000401"
     CDS             53914..54252
                     /locus_tag="U2R41_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887839.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            54242..54607
                     /locus_tag="U2R41_000402"
     CDS             54242..54607
                     /locus_tag="U2R41_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM8887840.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            54614..55240
                     /locus_tag="U2R41_000403"
     CDS             54614..55240
                     /locus_tag="U2R41_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8887841.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            55240..55704
                     /locus_tag="U2R41_000404"
     CDS             55240..55704
                     /locus_tag="U2R41_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887842.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            55899..58208
                     /locus_tag="U2R41_000405"
     CDS             55899..58208
                     /locus_tag="U2R41_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEM8887843.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            58220..58924
                     /locus_tag="U2R41_000406"
     CDS             58220..58924
                     /locus_tag="U2R41_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8887844.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            58921..61680
                     /locus_tag="U2R41_000407"
     CDS             58921..61680
                     /locus_tag="U2R41_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8887845.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            61693..>61922
                     /locus_tag="U2R41_000408"
     CDS             61693..>61922
                     /locus_tag="U2R41_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM8887846.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        21176 a        10165 c        12838 g        17743 t
ORIGIN      
        1 aaaggctttt tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg
       61 gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt tagttagtgg
      121 aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga
      181 cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc
      241 ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa
      301 ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg
      361 gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga
      421 aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt
      481 tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt
      541 gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa
      601 catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg
      661 atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc
      721 aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg
      781 ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact
      841 tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg
      901 atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag
      961 atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta
     1021 atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt
     1081 ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg
     1141 gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc
     1201 atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg
     1261 aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga atcagcgtaa
     1321 tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg
     1381 tacaagatct accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag
     1441 tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac
     1501 gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca
     1561 gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa
     1621 caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa
     1681 cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt
     1741 gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa
     1801 atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa
     1861 gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg
     1921 tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg
     1981 cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt
     2041 ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc gtatatcgtt
     2101 attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga
     2161 agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca
     2221 gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat
     2281 aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat
     2341 ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg
     2401 tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc
     2461 gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg
     2521 atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat
     2581 gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt
     2641 aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg
     2701 gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta
     2761 caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg
     2821 acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact
     2881 ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg
     2941 gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc
     3001 gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat
     3061 gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt
     3121 tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat
     3181 aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta
     3241 aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg
     3301 gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca
     3361 atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca
     3421 actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa
     3481 tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta
     3541 cctggataca atgtatcggc aaaaaacgga acagcccaaa tcgcttctga tacaggggga
     3601 tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat
     3661 ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg
     3721 gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat
     3781 tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg
     3841 gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt
     3901 aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg
     3961 atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat
     4021 atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt
     4081 gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta
     4141 gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc
     4201 cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca
     4261 aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc
     4321 cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg
     4381 ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc
     4441 gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat
     4501 gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct
     4561 tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt
     4621 aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa
     4681 tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta
     4741 cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt
     4801 aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga
     4861 tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga
     4921 atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca
     4981 agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca
     5041 ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat
     5101 tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa
     5161 tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg
     5221 cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg
     5281 acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc
     5341 atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct
     5401 acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg
     5461 agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa
     5521 caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac
     5581 gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag
     5641 ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc
     5701 atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag
     5761 aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc
     5821 ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc
     5881 ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact
     5941 ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag
     6001 aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac
     6061 gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc
     6121 aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc
     6181 taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata
     6241 catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa
     6301 caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg
     6361 attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt
     6421 tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca
     6481 gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg
     6541 aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc
     6601 aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt
     6661 ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga
     6721 tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc
     6781 cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga
     6841 tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat
     6901 gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc
     6961 gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt
     7021 aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat
     7081 ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc
     7141 aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat
     7201 gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat
     7261 attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg
     7321 gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg
     7381 ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt
     7441 cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag
     7501 acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa
     7561 gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa
     7621 aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt
     7681 cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac
     7741 aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag
     7801 attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa
     7861 aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt
     7921 ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag
     7981 ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt
     8041 atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca
     8101 tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta
     8161 cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca
     8221 aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa
     8281 aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa
     8341 atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat
     8401 taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt
     8461 acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag
     8521 ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag
     8581 atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta
     8641 gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg
     8701 cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat
     8761 cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag
     8821 ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt
     8881 aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct
     8941 attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta
     9001 ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac
     9061 atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg
     9121 aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca
     9181 agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt
     9241 aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt
     9301 taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct
     9361 tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt
     9421 cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta
     9481 cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa
     9541 tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct
     9601 ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac
     9661 aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga
     9721 atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt
     9781 gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag aatttccagt
     9841 agatgtgatt ggtcagtcag aaccagtgaa aggcgatgaa cgctacttgt cagaaattat
     9901 ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc
     9961 attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt
    10021 ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg
    10081 gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag
    10141 tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat
    10201 ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat
    10261 ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt
    10321 ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg
    10381 ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa
    10441 atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca
    10501 cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat
    10561 atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa
    10621 gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa
    10681 tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg
    10741 gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc
    10801 gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct
    10861 caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg
    10921 aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag
    10981 gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat
    11041 tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa
    11101 tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt cttaacatca
    11161 caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg
    11221 cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg
    11281 aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta
    11341 gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg
    11401 atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac
    11461 gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag
    11521 aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc
    11581 caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg
    11641 cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa
    11701 gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga
    11761 gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat
    11821 ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta
    11881 aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa
    11941 gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt
    12001 gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct
    12061 gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat
    12121 gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa
    12181 ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt
    12241 gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa
    12301 aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc
    12361 tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc
    12421 aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca
    12481 tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga
    12541 aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac
    12601 agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga
    12661 accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac
    12721 cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac
    12781 aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg
    12841 tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt
    12901 gtttgatctg taagaggagg aactacgatt tatactattg cactattact tcatttactt
    12961 caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt
    13021 ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc
    13081 agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca
    13141 atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc
    13201 catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa
    13261 attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg
    13321 gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt
    13381 gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc
    13441 atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat
    13501 tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa
    13561 tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc
    13621 tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag
    13681 gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa
    13741 ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca
    13801 gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg
    13861 tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct
    13921 gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc
    13981 caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt
    14041 aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga
    14101 cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag
    14161 ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac
    14221 attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca
    14281 tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga
    14341 tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac
    14401 atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa
    14461 aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc
    14521 aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg
    14581 aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt
    14641 tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag
    14701 aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa
    14761 atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg
    14821 aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat
    14881 tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat
    14941 gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt
    15001 gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat
    15061 taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa
    15121 aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta
    15181 gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat
    15241 tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac
    15301 catatgttcc aggatgggat acacatggat tgccgattga acaagtattg gctaataaag
    15361 gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac
    15421 tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg
    15481 aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta
    15541 aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa
    15601 gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt
    15661 ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca
    15721 tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat
    15781 ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag
    15841 aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac
    15901 aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt
    15961 taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg
    16021 gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact
    16081 caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa
    16141 atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta
    16201 ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac
    16261 aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag
    16321 agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt
    16381 gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg
    16441 gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg
    16501 gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc
    16561 caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt
    16621 ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata
    16681 tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg
    16741 tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg
    16801 gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca
    16861 aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg
    16921 ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata
    16981 cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt
    17041 acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag
    17101 aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga
    17161 acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata
    17221 ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg
    17281 tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat
    17341 tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg
    17401 caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct
    17461 taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac
    17521 taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg
    17581 cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc
    17641 cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa
    17701 cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc
    17761 tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat
    17821 tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt
    17881 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
    17941 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
    18001 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
    18061 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
    18121 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
    18181 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
    18241 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
    18301 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
    18361 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
    18421 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
    18481 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
    18541 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
    18601 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
    18661 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
    18721 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
    18781 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
    18841 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
    18901 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
    18961 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
    19021 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
    19081 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
    19141 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
    19201 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
    19261 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
    19321 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
    19381 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
    19441 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
    19501 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
    19561 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
    19621 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
    19681 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
    19741 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
    19801 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
    19861 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
    19921 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
    19981 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
    20041 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
    20101 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
    20161 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
    20221 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
    20281 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
    20341 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
    20401 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
    20461 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
    20521 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
    20581 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
    20641 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
    20701 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
    20761 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
    20821 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
    20881 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
    20941 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
    21001 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
    21061 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
    21121 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
    21181 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
    21241 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
    21301 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
    21361 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
    21421 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
    21481 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
    21541 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
    21601 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
    21661 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
    21721 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
    21781 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
    21841 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
    21901 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
    21961 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
    22021 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
    22081 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
    22141 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
    22201 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
    22261 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
    22321 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
    22381 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
    22441 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
    22501 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
    22561 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
    22621 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
    22681 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
    22741 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
    22801 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
    22861 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
    22921 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
    22981 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
    23041 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
    23101 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
    23161 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
    23221 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
    23281 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
    23341 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
    23401 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
    23461 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
    23521 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
    23581 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
    23641 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
    23701 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
    23761 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
    23821 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
    23881 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
    23941 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
    24001 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
    24061 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
    24121 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
    24181 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
    24241 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
    24301 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
    24361 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
    24421 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
    24481 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
    24541 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
    24601 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
    24661 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
    24721 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
    24781 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
    24841 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
    24901 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
    24961 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
    25021 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
    25081 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
    25141 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
    25201 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
    25261 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
    25321 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
    25381 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
    25441 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
    25501 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
    25561 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
    25621 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
    25681 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
    25741 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
    25801 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
    25861 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
    25921 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
    25981 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
    26041 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
    26101 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
    26161 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
    26221 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
    26281 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
    26341 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
    26401 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
    26461 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
    26521 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
    26581 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
    26641 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
    26701 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
    26761 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
    26821 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
    26881 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
    26941 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
    27001 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
    27061 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
    27121 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
    27181 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
    27241 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
    27301 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
    27361 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
    27421 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
    27481 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
    27541 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
    27601 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
    27661 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
    27721 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
    27781 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
    27841 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    27901 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    27961 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    28021 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    28081 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    28141 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    28201 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    28261 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    28321 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    28381 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    28441 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    28501 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    28561 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    28621 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    28681 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    28741 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    28801 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    28861 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    28921 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    28981 gttcctcata atgaattttc taggaggaac tgaaatgatt acaattgcca gtgcaatgca
    29041 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    29101 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    29161 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    29221 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    29281 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    29341 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    29401 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    29461 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    29521 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    29581 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    29641 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    29701 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    29761 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    29821 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    29881 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    29941 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    30001 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    30061 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    30121 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    30181 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    30241 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    30301 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    30361 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    30421 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    30481 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    30541 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    30601 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    30661 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    30721 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    30781 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    30841 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    30901 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    30961 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    31021 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    31081 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    31141 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    31201 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    31261 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    31321 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    31381 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    31441 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    31501 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    31561 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    31621 caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg
    31681 ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt
    31741 tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt
    31801 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
    31861 aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt
    31921 cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta
    31981 agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat aattcttttt
    32041 gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa attttcctat
    32101 ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat gttttattaa
    32161 tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa
    32221 atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac tgcgcagaaa
    32281 ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt
    32341 cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga tcaaacggaa
    32401 ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta aacttaataa
    32461 cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat cccactctat
    32521 ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt
    32581 attttctata agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa
    32641 agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt
    32701 ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat tttcttatct
    32761 tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt
    32821 tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag
    32881 aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc ggcgcaagga
    32941 tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc aatcagaggt
    33001 gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt ttagattatt
    33061 tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc tagatgtcat
    33121 attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa atgagatatt
    33181 ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat cgttttttct
    33241 aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg tgaatagcca
    33301 gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca tgctaacaat
    33361 tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc gttatcccat
    33421 gaagcgctgt taattcgaag taaacaattc ttataaagat ttttatacat atctatttta
    33481 ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc aatgagttgg
    33541 tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt ttctaatgat
    33601 tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt tttagaccca
    33661 aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc gccttcaact
    33721 attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat agttacctcc
    33781 tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt gtataggttt
    33841 tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt tacttacttt
    33901 actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc ttgaactaat
    33961 ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt ttaagtcctt
    34021 cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac ttttatcatc
    34081 gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa cagttccttc
    34141 aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct cagtagctgg
    34201 agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg tctctaaaaa
    34261 ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt gagtatcaga
    34321 tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa gcgagaaacc
    34381 tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct tatttgtttt
    34441 tttgataaat cttacaatta acatgattaa acctataata aataaaacaa taccagtaaa
    34501 accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat attataaacc
    34561 aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt catctaacaa
    34621 ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg aattattatc
    34681 taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat tccaatattt
    34741 ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc cgcgactttc
    34801 gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt tttttaattg
    34861 tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt gattaactaa
    34921 atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa tcaaggtttt
    34981 tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc tgtgatatga
    35041 ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg ggctttttta
    35101 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    35161 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    35221 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    35281 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    35341 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc
    35401 gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt
    35461 ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat
    35521 ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac
    35581 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    35641 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
    35701 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa
    35761 tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga
    35821 taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc
    35881 gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac
    35941 aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac
    36001 tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa
    36061 atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct
    36121 tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt
    36181 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
    36241 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
    36301 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
    36361 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
    36421 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    36481 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
    36541 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
    36601 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
    36661 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
    36721 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
    36781 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
    36841 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
    36901 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
    36961 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    37021 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
    37081 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
    37141 gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat
    37201 tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct
    37261 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    37321 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    37381 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat
    37441 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    37501 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    37561 agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg gggaagcagg
    37621 caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct tgatgaagct
    37681 gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg aatagaaaag
    37741 agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt tctctattgg
    37801 gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta gagtaaatat
    37861 catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt caaatgcaag
    37921 gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag agtatattct
    37981 attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag ctgaccatct
    38041 gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt ctgttgaata
    38101 gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag cattaagttc
    38161 aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg tactcaatat
    38221 aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca aaataaacac
    38281 caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa gaattagtaa
    38341 ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa
    38401 aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc aattttggac
    38461 agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga gttttcttca
    38521 ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa gctatctact
    38581 tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc aagaatactg
    38641 ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa gatgttaaaa
    38701 aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta
    38761 agcaaaaagg aaatatgaaa gttatgggag ttctaggggg aaaagaaagc cgagcttatg
    38821 aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt accttttcaa
    38881 tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca tttattgaaa
    38941 tgtggttgcc agaaactgcg atccgtatgg aaattgatca actgaacaga caacagagac
    39001 tttttggtga tgacaatgaa tagagctgaa gcgctaagaa tagggacggc aattgctaat
    39061 tgctggtgga aatactataa accaatcatc ctaagccaac aacatattga caagcaaaaa
    39121 gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa agaaaacaca
    39181 tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa tatttgattg
    39241 atcctgattg gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc
    39301 ttttgacgaa tgaagaacta gcttgggatc aagatgattt aggcaaccat gtaactattt
    39361 gggatcatgt ttatcttatc catctatcga agcattcgaa taaacctgaa tatatttacg
    39421 tcatcgaaga tggcttgatt gatgcgctag aagagtacga cagagataac ttgattgata
    39481 tctcttatta cggaccaggt aagaaataca ttgctgaaat ggaggcagaa tttgatgagt
    39541 gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc
    39601 aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat tatgaaaaag
    39661 aatgatccac aggcaacggt aactattaat gagtacaaac actacagggt tgtttctgga
    39721 actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga aactgggact
    39781 tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt tcctgtttta
    39841 gattatcgaa accaaccagt tgttaaacca aatgcaatgc aaatcaataa ctcattgaag
    39901 cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt tcaaggggaa
    39961 gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga gacgattcta
    40021 gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga atatgttaat
    40081 gagcagacag ataaattagg gctcttagct gataacgttg aaactattga acagttaagc
    40141 tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga attagataag
    40201 aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg aaaaagttca
    40261 aaggaaaaga cataagcgca accatcaatg aggaagtgaa cattgatcga ctaaagacga
    40321 tgtatgacgg ctacgatggt gatcgagtca ttgaaattcg ttttattgat cctagacgtt
    40381 tcaccgtaca gcaacgaaac ttcatctatg cgctgatagg cgatattttt atcgatacag
    40441 gcatgccaac ggacttctgg aaggaattct tctactttcg ttttgaaggt gtcacagggc
    40501 gcaaaataag cctgaaagac gaatcgaata caactgtgag tgatgccaat gtcttagcaa
    40561 atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt
    40621 tacctgcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct
    40681 gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca
    40741 aagaagttga tcatgcagag tacacatttg cagcactctg cagaatccat cacacagaga
    40801 agcacaaaat aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg atcaaattaa
    40861 accagaaaac aatcaaaaag ttaaatattg gagggtaaaa atgacagaac atcgaagtta
    40921 ttatgcgatt ataccagcca acgtaaggta cgacaaaaga cttaaaccaa atactaagtt
    40981 gttatacgga gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga
    41041 gtactttgca gatttatatg gtgtgaataa agagaccata tcgcgatggg taagtgattt
    41101 gattaagttt ggatacttga atcgggaaat catttacaaa gagggtacca atcaaataat
    41161 caataggtac ctacgaatta atcaataccc tattgacgaa aaacgcaata cccctattga
    41221 cgaaaaagtc aaagataata atacatctat taataataca tttaataata caaaagaata
    41281 tataagagag ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg
    41341 agagtataaa aatgttcttt tgtcagatga gcagatggag aaactcaaaa cagaattccc
    41401 taatgattac caagagcgaa tcgaacgact gtcagagtat tgtgaatcat ctggtaagac
    41461 ttataaaaac tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa
    41521 gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg aatgggcaat
    41581 cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc
    41641 accattctaa gaggtggaaa aatgaagatc gattatctag aactaattaa tgaaatagcg
    41701 aattataaaa agggcgagga attagacgtc ctgagagacg tatatgatca actcgaagaa
    41761 gctggaattg aaggaattaa gaatgatcgt tcgagttgga gtaaactcag atactatttc
    41821 gcactctata tcgatggaac gcaattaaga aatttagcat ataccaaatt actattcatt
    41881 gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta aacaagatgg
    41941 acctaaagac atttacagca cagatcgaat taatgcatca agaagcttta agacaaagtg
    42001 tgtcgtacga agacaagtgg ctcaacacat ttcatggcgg acgtgagagc gcacttgatc
    42061 aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc aatgcagcga
    42121 attatcgaat tgacttattc agaagattgg caggaagata aaaaaatagt tgcagaagtc
    42181 caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc gagaaagatt
    42241 gcaatctggc atggtgatcg aattctagta acgggtaccg ctaaacagtt atctgaaatt
    42301 actggtctga gcaaaaatat tatttgggat agagctagga gcttatggat tgattcaaaa
    42361 ggaagacagt ttaggtatgt ggaggagaaa taatggacga actaatcgcg aaagtagagc
    42421 agtgggcaaa agataaggga ttggatcaag cggatttcag caagcaaatg ttaaaaacgg
    42481 tcgaagaaat cggggaagtt gccgcttctc tagctagaaa agatgaacat ggtttaagag
    42541 acggaattgg cgatgtagta gtaaccttga tcatcttggc aatgcaaaat gatatggatt
    42601 tatacgagtg cttggatttt gcttatgacg aaatcaaagg tcggactgga aagatggtca
    42661 atggtgtttt cgttaagtcc agtgacctga aataattcca ttaatggaag aaataactga
    42721 ataggaggat agacatgatt gatgcaaaac aagtgcaaaa acagaaagat ggaatgttga
    42781 tgtttgaagc atatgttcta ccatttctta atcaatttga agttttggaa tgctctgcca
    42841 gcggagaaga attggagtat gttgtaattc gtgaaaccaa agaaaacgtt cagaagctaa
    42901 acgaatttct ttgcactatt aactgttggg atatgatagc tccaggattt ttatgtccag
    42961 ctatgggaga atttttggaa tattgtcgtc ttgaagatgc aggcactttg gatttagctt
    43021 atctggtata caactaccta aacatcaaca cagatcattt gtggtttggc actgccgaaa
    43081 gaaaatgggt agtccgctaa ccaccaatat caatgaaaaa aggcggtttc ctgaaagtgg
    43141 aattgccgcc attgctgtaa ggttctttac gcgactcaag gtcacagaaa tataaatgca
    43201 aatctctttt gaagtgatcc aaagctctcg gaaaatggaa gttttttgtt tcaaagaata
    43261 aatgagattt gcgtcaagaa cctgagagtt atttccaaaa aagcttccaa agaaccgaat
    43321 gagctaagga catcatgtgt aaacacataa tatagctctc ctttcacggc taatcataac
    43381 atgaaaaata ttcgatgtca gtaggaaaaa aggtgaatta tgaaaagaat attaaattat
    43441 cccggcagta acagattctg caattaaact ccgaagctga tgtatcagta tcaactgtag
    43501 aatattatga gcgtggagaa agtcaagcag tcacaaatgt aaaaatcgat aacgtcaacg
    43561 gtcttatctg tttagatgaa ggcgatagta tgaatgaaat tgctgcagac catgaattat
    43621 cagaaactgc tttaattgat caaatcgaat tagctagaca atactttgtt ccatatgcag
    43681 atacctggtc caagcataag aatgacatca tttttccaga ggaacaatga gagcaactga
    43741 tccagtaatt atccttgagg aagccaaatt tatttggact cacgaagaga tagagcaagc
    43801 acgcttgctc ttttctcaag gagttaagcc gagcaaagta gctgaaataa tgggtcaaaa
    43861 gattcttgat gtcggattgc ttttgctcca tctagcagaa aaaaatttga tttgaggtga
    43921 aaatgatgat tcaacttgca ggcatccaaa cagggaaaat ttatttttct ggagaaagca
    43981 aaagcgaagc caaccaatgg ctattgaaaa cttacacgaa caataagaag ctaaggaaaa
    44041 aatatcctga tttgttgcta aaggatgatc agattatgcc ggaaccgatg attttgaccc
    44101 gtaaggaggg aagttgaaac taaaaaacca gccgtgtggg ctggagttgc tatttctttt
    44161 taataatgag tttttctaaa tttaattcat tctcacgagt ttgcgtccaa taatcagaat
    44221 aatattttaa ttgtttaaga gtaggaatgt cgtcttcggt aaaattagaa aataattctg
    44281 gagaattatc tttttttaat tgttctaatc ctgattcttt aaaacgttgt tcaacgagtt
    44341 ttactaaatt gttgatattc atgataaatc acctttttaa atttaatttc gatttgatta
    44401 atcgatacaa gtattatatc aaataaaaaa gctgatttag ggggtcttac aaaaacacta
    44461 aaattttttc aaaaagagga ggaacggtgg tgaaagtcag agaacaattc aagatagtga
    44521 gcgacgaaga taatgtttac aaaataaaga tcgttaatat taatgaatat cgagaagcag
    44581 atcacaaata tttggttgac ttatacatga acgataaata tttgggagaa ggattttgtg
    44641 gagattcact ttttgatgat gagcgaattt tgaaggagga aacagaatga tccacgaact
    44701 aaaaatacta ccagaatatt ttgaagcagt gacgagcgga cgcaaacaat tcgagatccg
    44761 caagaatgat cgtgacttca aagttggtga tcagttaata ctacgtgaat ataaaaaata
    44821 cattaaacat gaattacctg gactaacgag accaacattt agttacactg gtgactcata
    44881 caaagcagag atcacttata ttactgatta catgcagaag tccggatatg tagtgttggg
    44941 gattaaaggg gtgaattaaa tgaaagtata catcttagct atcacagagg gcacatggat
    45001 gttcccagtc ggttccggaa aaatctataa atcaaaaaca gctgcttata aagcatttga
    45061 aaaatacaaa aaagaaaacg gtggcggcac taacgcaaag attctagtgg ctgataactg
    45121 gcatgaagaa ggtgaacgta attgatttac ctgttcattt tcgtggtact aactattttt
    45181 atgctagtaa tcttattcaa aaaaagatga gtaaagacga acgaaaaatt ctggccaact
    45241 tttatttagt attaaaaatc gaacaaaatg tgttttcgga aaggagaatt gtatgggaaa
    45301 aacaaaatca aagattaaga agaaaaaacg acgtttggaa caaaaggcaa tccagaatgg
    45361 aacggctaag aaaaaataaa aaagctaccc ctttcgagat agcagcgcaa tactattcta
    45421 ccataagggg tggcgtttgt gagatttcat tggttaaaag attaccaaga actagatgag
    45481 cagattcttt acttaaagtg gaatcttaat aaaagtaagc ttgaattgaa tcgatgggtc
    45541 tgtggtgatt tagcaaacgt ccgcatcgaa aagaattcga gaacttcttc tttagaagag
    45601 aatatccaaa agatagaaaa tgaattggaa ttgttgatcg agcaaaaaga agaaatgtta
    45661 ttgctgatag atagcttttc tggtatcgat aatcaaattg ttaagatgaa atatgttgat
    45721 caaatgagct tagaagatat tgctgaatct gttgggtata gctcatcgta tgtcagacag
    45781 cgacatgcag aaattagaaa gacattgaac tttttagatg agtatgaaca cagacaagct
    45841 gaccgattga aaaaagaaaa cgaaattgat ttttacaata gcgagaagta caaagaacaa
    45901 ctctctttat tctaaaatta cactgttcac taaatgttcg gtcattgtag ctatgtaaac
    45961 attgttagca gcatgatatt ctattagtgt caaaaaaata tgaaagagcc aagatatccc
    46021 aaccgtttta ttaattggta tctgtggctc ttttattctt ttaattagta gttggcataa
    46081 agttatttcc atttataaat tgatcagcta aatcagcatc ataatcagcc gcgataaatt
    46141 gcgctgctga tttaataaat gcatccatat catgaacatc tttattagta tgtctacgaa
    46201 catagtgcgt ttcatcatta ccaatccaag tagcagcttt ggctaatctt tggagtttag
    46261 gaaaatcatt caaataattt tcaattgttt tacctaataa catttttttt attttatctt
    46321 catcctctga atttttagat attgcataat ctttaattag aaattctaga gcttttctat
    46381 aaccaactcc agcaatttgt tccaattgct cagatttagc ttgtgtggct tgagtataaa
    46441 tatcaacaaa tgtaggagaa actttttcaa tattttctgg taaatctatt ttaatcggcg
    46501 gacggtaata atctttaatt aaaatatttt cagaatatcc ttcatcttca ttaaacgtat
    46561 ttataaattg taaactataa aatttgttgc actcatcatt tatacactgt actaatacgc
    46621 caaagattct atcattatcg aacgcactat atgatgaaac cccaaaatac acatacggag
    46681 tcattatatt tccgcaatgc ggacaactat ttggcaaata aattgtctct gtattttgac
    46741 gatcacgata cttaatatta acttcttgaa gcattaaaac acctcgcatc ttttttataa
    46801 atcataccac aaaatatctg ataggagagt aaacaaatga atgattttca tgaagcggta
    46861 cttagtattg aagtagaatc aagtttggct aaagtttata agaaagcaat tgaagcagag
    46921 aatagtccgt acagagaaaa ttggaatggt aatcatgcac atgtgcaagt ggagaatgat
    46981 gattacagaa ctggaatgaa tacgttagtt atttctttat tgtcacacac tctacccaat
    47041 ctgcaggaaa cgattgaatg gtatgagcga atgggcgcaa aagtaatccg tacaaattac
    47101 aaaggagaga attaaaatgg caatgatcaa aattagaaca tccattagtg gaacagagta
    47161 ttgggattca gaaaagaaaa ggacagtagt tgttccgaaa gatcaagaac caaattttga
    47221 agtaaaagaa gaaaaaggaa ttttgagtga tggcaaatca tttgttgctg tcaatggcga
    47281 actcatagct catagcagtg aaattcttga tagcgatgga aacactgctg ctaattttga
    47341 tggagacgaa gctactaatg atcagtccac tgaagatacg gatgaactgg acaatatgac
    47401 tgcaaaagaa ttgcgtgcat atgctaagaa acatggtatt gatattcctg gtgctatccg
    47461 tgcaaaaggc gatatcataa atcttattcg tgaagtagaa tgaaatactg tcagtttgac
    47521 ggatgcacga acaaaatagc aaagggtatc tactgtactg aacacaagag atcaagcaga
    47581 tcacgcaaga agaagcagca agcaaagtct gtttatcatc atgagaacaa accattctat
    47641 cgaacgcaag catggaaaga tatgcgtcaa tttatttatg aaagagaagg tggtcattgt
    47701 cagcggtgtg gtcagttcat atttggaaag agagcacacg tccatcacat tgtaccaatc
    47761 aaagacaacg aactgcttaa gcttgatcca aacaatctca tgctattatg ttcaaaatgt
    47821 catccaattg ttgaaaacga aacggaagac aaaaaagttt ttccttcgta tttcaattga
    47881 agccccccta ttcattttca aaattttttc gcgtggggag atagggtagc ggggagtcac
    47941 gcgcatcgtt aggtcaaatt tttcaaaaaa caaagggggg tgtatacaaa aatgacgact
    48001 aaagcgcaac gtaaagcgat tattgatgaa aaagtaagtg ctgaaaaagc tcgtatctta
    48061 gaaataatga atttgtctga tttgtacacc atcactcttg atccattaat cgaatcatac
    48121 ttggatattt ttgaaattta ccaacacaaa tatttattgt ggaaggaaaa aggatttccg
    48181 gagacgcaaa aattcacgaa taaatcagga gctactaatc aatcaaaaca tccattggca
    48241 caacaagtag aaacttgggc agataagaag atgaaagctc tagatttatt aggattaaca
    48301 aacaaagcaa aaactggtag actaattgct ggaggatcga ccgcaagaaa agatgaagaa
    48361 attacacgcc cagaagtaaa gccagtagat gaactagcag cgcacagaaa taaatggcgt
    48421 aagaaggcag gtgctgaaaa atgattgaac ctggtgtaaa ttatgctgat ttatttgcaa
    48481 aagaagtaag aaagaaacct aagaagtatc ctaagaccgt tcgtttagca atagatcgct
    48541 ggtatcagtg gaaaaaaaga aaagatattt ggtttgatgt agatcgcgcg aatgaaatga
    48601 tggattgggt agaatcattt attgttcaca caaaaggaga aatggtggga aaaccgtttc
    48661 ttttagaacc ctgggaaaaa tttatttatt cttggattta tggttgggtt aaagaaaatg
    48721 aaaaaggcca aattgtacgc gttactcgtg aggcttatgt ccagattcca aaaaagaatg
    48781 gtaaaacatt gattgctgtc ggttcattag gatatgcaat gtacggagaa ggggcgttat
    48841 cagttgattg ctatgcttgc gcatctgatt ttgctcaagc gcaatatgct gccaaacctt
    48901 ttgcagttac aatcttaaac aatccaatcc tacttgaagg aactaaaata tttagggggc
    48961 caaagggaac tgtttctagt attacttatg actatattca tgaagatatg gcttactcaa
    49021 ataaatttat tgttcaaacc aagaatattg ataatatcga gggatccaat ccatattttg
    49081 tattgaacga tgaattgcat aaacaggaga aaatggaaca gtatgacaat tttaagtctg
    49141 ctcagatatc tttaccacag ccattgatgt ttaatatctc aactgctggg aaaggttcgt
    49201 cgtctgttgg aatgcgtgta tatcgcgaag caaaagaagt gttgaaacgt gatgataatg
    49261 attcaaactt tgttctaatc tatgagccaa ataaaaatta tgattggacg gatagaaaag
    49321 tctgggaaat gtgcaatcct aactggggaa tatcagtcga tttatctgct ttagaatcgg
    49381 catttaaaac agcgcaacgg tcagctcatt ccaaagctga atttctaacg aaacacttgg
    49441 atgtatttgt gaacggcgcg gataatttct ttgaacagga tcaagtagag ccatgtttag
    49501 ttaccacaca agaacttggt gatttaagtg gcgaaccatg ttatatcggt ttagatttat
    49561 cacgtacacg agatttgacc tgtgtatctt taaacttccc aacatgggat gaagatggta
    49621 aagcggttct taaagtaaag caactttatt ttattccgaa tgaagattta gagtttcgag
    49681 aaaaagaaga taatgttcct tacagtgatt tggctgaaca aggtttcgtt gaattttgcg
    49741 acggaaaaat gattgatcaa gatcagattt tgcagtacat agaggactgc atgaatttat
    49801 atgatattca gcaagtgaat tatgatccag cgatgagtga caaacttgtt gagaaattag
    49861 aaaacttagg attggaatgc gttgaagttg ctcaatatcc taaagtattg aatgctcctt
    49921 ttgacgatgt cgaacggttg ttttatgaga aacgaattca atttgataat ccattattcc
    49981 tttattgcac tttgaacgtt gtagcaatta ctaacatcaa tggacaaaaa gcgccaagta
    50041 aacgtcaatc aaagaaaaag attgatgggt tcgtggcgtt tttgtgtggt cataaggaaa
    50101 cgatgaatca aatgacagat attgattcag atgagttaga tgattaccta agttccatct
    50161 atagataaat agaaaggcgg tgaaaaaaat tgaaattacg tgatcggtta tcgaatgcag
    50221 tctattcttt tatggaaaaa cgtggatata tcgaggatat ttttggacac tacacacgtt
    50281 atggtcaaag atatgtgacg gattcatcta tcatggaatc ttctgatgtt tatgaattag
    50341 ttcaagacat atctaatcag gtggcattgg ccacaccaat tgttattggt cccgacggca
    50401 acgaagtcaa agatcatcac ttactaaata ttttgaagag tcccaatgat tatttgacag
    50461 gattcgaatt taccaaattg gaaacaaata ctttattgat caatggtgag acattcccat
    50521 taacggatag ggatcagctt catttggcgt acggtgtaac aactaaaatc aatgaacgac
    50581 ttcaagaaga atttgaaatg aatggtcaaa aaatacctgg tcaaatgatt cgacacatta
    50641 agaacatcgg aactgattca ttaaaaggtg ctggaataat tgatcttgca agaaacactc
    50701 tagaaggcgt tctgagcgct gaaaaagttt tgacggacaa atatactaaa ggcggtttac
    50761 ttgcgttcat gcttaaacta gacgcccaca tcaatccaaa taatagcgcc caaacaaaaa
    50821 ttgtcaaagc tatattggat caactggaag gaacgcaaaa tgagagtgat cattctgtta
    50881 agatgattcc tttgggaaaa ggatattcca tcgagacatt aaaaagtcct gttgatgacg
    50941 cggcaatttt gaattattta ggtgtttaca aaaaagactt aggaaaattt ctaggaatca
    51001 atgttgatac gtatcaatcg ctgatgaaga cagatattga aaaagcgatg atgtatctgc
    51061 acaacaaagc aatcaaacca atattgaaaa acaagagcga acattacacc gctctttttt
    51121 ttatgcctaa ttctggctat agagtggaat ggaaaattaa tattttggat tttgttcctt
    51181 actcaacaaa aacaaatatt ggctacaaca tcgttcgtac cgggattaca agtccagata
    51241 acgtggcaga aatgcttggt tttcctaaac agaatactcc agaaacacaa gctatctata
    51301 tttcaaatga cttatctagg attggccaga aaaatgcaac agatgattcc ttaccaacga
    51361 atgatcaaga cttgaaagga ggtgatggaa atgaagaaga aggaaattcg cacgattgac
    51421 atcaccaatc tttcaacacg ttccgatgaa gaaactcata cgaggaccat tagcggctac
    51481 gctgctgtat tcaacagtca aacactttta tgggatgact taagcgaagt gattgttcct
    51541 ggtgcattct caaaggcaat tagtaattcg gatgttcgtt gtttgttcaa tcacgattgg
    51601 tccaatgtgc taggacgtac taaaagcgga acccttcgcc ttgaagaaga tgaacgtggt
    51661 ttgaaattcg aagttgattt accggacaca acggtagcaa gggacttagt taaatctatg
    51721 gaacgaggag acatcaacca atgtagtttt ggttttgtgc cgactgaaga aacgtgggac
    51781 tacaattctg aaccaatgct tcgaacaatc catgaagtgg aattatacga ggtttctatt
    51841 gtgcctttgc cagcatacga agatacagaa gctgcattaa gaagtcgtga tgaattagaa
    51901 aaaaacgtcg aagaaagaaa aaaattaatc aaaaaaatta atcaagcgct agaagcgtag
    51961 gaggaaaaat acatggataa agaattattg aaaaaaatga aggcacgtcg tgagcaacga
    52021 ttgactgaat tacgtgaaaa agttgaatca ggagaattac gcgaagcaga tttagaagcc
    52081 gtgaaagaag aaattgacag tgttattgat gaattgaacg gaattaaaga cgaattaggc
    52141 gcagattctg gaactgatga aacagacgac aatacagatg atcaatcgaa tagtacggat
    52201 tcagacgaaa gccgttctgg tgaagacaac gatcaagaag aagattcaga cagtgaagat
    52261 agttcagaaa atcgttctgg aatgattact caacagcaac gagatggatt acttggatca
    52321 attaagaacg gattggaggc acgtgcaaaa atgaccaaga aacaaaaaga tcaacaacta
    52381 cgaaaagcat ttgctaattt tgtagctgga aatatttctg aagcagaagc tcgagcttta
    52441 gggattgaag ctggcaacgg ttcagttact gtcccagaag taattgcatc tgaagttatt
    52501 acttatgctc aagaagaaaa tttacttcgt aaatacggaa cagtggtgcg aacatcagga
    52561 gatgtcaaat atccaattct tgtgaagaaa gcagatgcga atgtaaacaa gaaagagcgt
    52621 tcaactgata ttgctgaaac agctattcag tttgatgaaa ttttgcttaa tcctgccgaa
    52681 ttcgatgctt tggcaacagt aactaaaaaa ttattaaaaa tgtctggtgt tccagttgaa
    52741 gatattgttg tggaagaatt gaaaaaagct tatgtgcgta aagaaatcaa ttatatgttc
    52801 aatggtgatg acgctggaaa tgaaaatcct ggtgcattag ccaaaaaggc tgtagcattt
    52861 aaaaaacctt tagatctaac tgcttcaggt gctgggcaaa aattatatga tgcattaatc
    52921 gaatttaaaa atacaccagt gacagaagtg atgaaaaagg gacgctttat tattaatcga
    52981 gctgctttga ctgccattga aaaaatgaaa acagatgatg gatttccttt gttgcggcca
    53041 ttcacacaag cagaaggtgg aattggttac caattagttg gctatcctgt ggattggaca
    53101 gatgcagcag ataaaaaggg tgaaccagac acgccagttt tatattttgg cgatttttct
    53161 gcatttaaaa ttcaagaagt tattggtgcc ttggaaattc aaaaacttgt tgaaaaattc
    53221 tctggtaaaa atcaaattgg attccaaatt tacaacttgc tagatggtca attggtttat
    53281 tctccatttg aaccggcagt atatcgctac gaaattacaa aaccagttgg tggttaagat
    53341 ggaagagcaa actaaagaat tgtctttaga ggaaaaattc aaatcacata ttcattttga
    53401 agagggcatg gatgattctt tgctctcttt ttatttaaat atggcaaaag attatgtcaa
    53461 aacagcaact ggtggccaac aagaatatct tattttgatg gttgccggca ttgcctatga
    53521 atatagagtt tcagaggatg aactcgacaa agctatgaat gccatgacgc catttatcgt
    53581 gcaaggagcg attcaaaatg ccgaagagac agaccaataa tctcagatgg aaagccgaat
    53641 tgctagacat caaaacagga acagacggaa acgatcgtcc aactacaatt tacgaattta
    53701 agcgtctaat attctatgaa gaactcggtg tgacttctca agaaaaatat ttatcacagc
    53761 aagccaagac agacgttgtc agacgaatta aagtcagatg ggataaatcc atcacagaga
    53821 aattaagtgc gctcaaaatt gattctgtaa cgtataacat tactcgcatt tatacgaatc
    53881 ccgatgcaag agaaatggag ttgagtttag cttatgtcga ttagctttga tgaattgaaa
    53941 acagcgctga aatcaacaaa gttagcagtg ttcagagaca aagccagatt agggacgatg
    54001 tatccataca ttgtgtactc aaatgtgagt aacagcaaaa aaatggcatc cggtaaagtc
    54061 tataaaaaat taccttatta ccaagtctct ttttttacac ttggaacaga acaggattta
    54121 gttgttattg aacaagcgct gcaaaatgct ggcattccat attcagattt tacaggtata
    54181 caaggtgatg agaacgacga taccgtaacc aactattaca catatgtgag gtgtgtggaa
    54241 aatgccaagt aataagaatg gtttttctga aatatcggat tatttaggaa acctttcaaa
    54301 agttgatccg aaaaaattat cgttggaatc tttagaagaa gcagctaact tttatctaaa
    54361 gcagttgctt cctaatattc ctaagtcgct cttaaaaaag aaacatatgc gcgatcaaat
    54421 aaaagtagta gttgaaaacg atcaagtgaa agttcaattt gaagagacgg ccttttactg
    54481 gcgctttgcc gaaaatggca caacaaatca acgtgcccaa cattttgcaa gtggtacgta
    54541 tgaacaaaac aaagaaaaaa ttgaagaaat tatgactaga aaaatacttg atttatggga
    54601 agggtgattt taattgggaa aacaagatac tttttatttt gaaggattgg acgacatttt
    54661 aattgcgatg atggcaacgc cagattctgt tggaactgca ccgacttata gtgaagtttt
    54721 acgattgcca attgccacaa aaatcggtgt caaagggaat ggaacagcat tagaaaaatg
    54781 ggcatccagc aaaatgtttc gtcgtgtgtc gcgagaaaca aaacatgaac ttgcgttgga
    54841 tcacgtgggt attccgatag aagtgatgga tgaaatcaaa ggattagttg ctaaaagcgg
    54901 agtaacgttt agcaaaaata ccgcacgaga atttccgtac ttcgcctttg gatttattgg
    54961 aaatattgaa aatggcggta aaaaagcagt ttggtatcct aatacacaac tatccaatgt
    55021 gattgacgaa gagtatgcca ctgcagagga agaaacaaaa attgacgatg taactgcaaa
    55081 tctggtttcc atcggactga aatacaataa tgtgatgtat gcaagttttg attcaaatag
    55141 agacggagct tctctagaat tatttaataa attcattgct caacctgtat acgatgaaga
    55201 acagtggaaa acattggcta aagttggagg tgcgggttaa tggctcggtt atctgattat
    55261 ggaattcacg ttgaagactt aaaaaattct gctgttgtaa ttattcaagg cgcagaattc
    55321 cctatttcat ttactatgca aacgatggaa tttatagcag atgtgtatgg tggagattat
    55381 tcgcaatttg aatctgatat gaacgccatg ctatctaaaa aagaaggaca aatttcttct
    55441 gctaacttat cgcctagtga tctaaaaatc atgcgtgcct tgatttatgc catgctgcgt
    55501 actggtggtt tagaggaaga tccagaaact attttcaaat ttttgggaat gagtggagag
    55561 gtgttgtctg cttatagtac ctgtatggaa atttttgcta gccagacatt tcaggtggaa
    55621 gacctaaaaa aatccaagaa gccacaagac tttcaaaaag cgcaagcaaa aagaaagaaa
    55681 aacaaaaaga atcggaagag ataggaactc cttggagttt ttatatttac gttgctctca
    55741 ctctattgaa ttggagtgag agtttctttt taaagtctac acctaacttg tggctcaaat
    55801 cttatttaca gtggttacaa caaaacaccg attttgaacc acctcaatct gtaactatgg
    55861 ataaatcgcc ttggtggtag aaaggagcgc taagataaat ggctggtaaa gaatctgatg
    55921 tcgttcttaa ttttaaaacg aatggcgaag tcagttattc gaaaacaatc aaagaaatca
    55981 acaaagaaat gaacttagcc gctgccgagt acaaaaacca agtgtctgcg atggacaagg
    56041 atgcaactca aacagaaaaa ttgcgagcgg ctaaacaaaa attagaaaaa caattaggat
    56101 tggctgaaca acgatctcaa atgctgagag aggaatatga aaaatctgtc aaagaaaccg
    56161 gtgaatattc tgctgaatca gaaaaacttt ataaacaatt actcaattct gaaaccggag
    56221 aaaataaact tcgtacagca ttagaacaga cgaacgatgc acttaaagaa caaggtgacg
    56281 tttctgttga tacagcaaaa aaactccaga aaatcgaaga aacaggtgag aaagtaaaag
    56341 gcgttggtga aaaaatgtct gttggagtaa ccgcgcctat tgtagcggca ggagcagcag
    56401 gacttgcagc atttggtgaa gttgacgagg cacttgatac catcattaca aaaaccggag
    56461 caacaggtga tcaagctgat agactttcac agtctttcaa aaacgttggt tcaaatactc
    56521 atttaccttt acaaacggtt ggggaagcta ttggtgaggt aaatacacaa tttggattca
    56581 tggataaaaa actggaagat tcaaccaatt atctcctaca gtacgctgaa atcaatgata
    56641 cagatgtttc gcaatcagca atatctgctc gacaagctat tgatgcttat ggactagaat
    56701 atgatgattt gaattctgtc cttgatgtaa caacgaaaac atcgcagaat actggtcaat
    56761 ctgtagacga cttgatgcaa aaagcaattg atggcgcacc acaaattaaa caattagggt
    56821 tgagctttgg tgaaggggtc actttacttg gacaatttga gcaatctggt gttgattcaa
    56881 gcgcagcttt aagtagttta tctaaagcaa cagtggctta tgcgaaagac ggaaaatctt
    56941 taagtcaagg actagaagaa ctacaagaca aggtaaaaaa tgcaggttct gaaacagaag
    57001 ccatcaatgc agcagcagaa gtattcggga ctaaaggcgg tcctagaatg gccgatgcaa
    57061 ttcgtagagg tactttgaac ctggaagatt tagcaaaaac tgctggagaa agtgggggag
    57121 ctgtaggaga cacattcgat gctactctcg acccaattga tcaagccgat caagctatga
    57181 acaatgcaaa attagcaatg gctgacgtag gtgaagcagt ccaagtaagc cttttgcctt
    57241 tttttgaaaa agcaacttcc ttattacagg aattctctaa atggtggggg tctctagatc
    57301 aagatacaaa gaattggatc ataacaattg ctggtatagc ggcagcagtt ggaccagtac
    57361 taatcgttct tggttcactt atgggatctg ttacaaaaat cgttggggaa gttaaaagtt
    57421 ttataggagt ttggcaagga cttgctggtt tatttggaat gtcgggaggc tggtttgcat
    57481 tagcagttat agctattggc gcattagtta ctggactaat ttgggcatat aataacgtta
    57541 aatggtttca tgatggagtg aattctttct tcaaaggtgt ttcagatgta gctgttgaaa
    57601 tatttaattt tttaagtggt tattttggtg gatttttcga tggtgttata gcaaatttca
    57661 ataattttta taatgctgga aaacgaattt tcagtggttt tctagatttt atcactggag
    57721 tatttacagg gaattggtca aaagcatggc aaggtctagt tgatattttt ggaggtattt
    57781 tcgatggtat tgttgctgta gggaaagctc ctattaatgc catgattgga ttgatcaatg
    57841 gatttattgg tggattgaat aatataaaga taccaaaatg ggttcctggt gtaggtggaa
    57901 aatcattttc tatttcaaaa ctgccttatt tagcacaagg cggtcatcta atcaatggac
    57961 aagcgattgt tggtgaagct ggacctgagt tactgactgc caagaatggt aaaaccacag
    58021 ttactccatt atcagacgaa gaaaaacgca aagggatcag tggaagagtt tctggaggta
    58081 gcattgaaca gcatatacac attggcaaag tcgatgcaaa caatccatct gagttagatc
    58141 gtatgaatcg taaatttgct aaagcaaaca aacaggctat tcgtgatttg ggaggtgttc
    58201 ctatttgagt cgacaattta tgaatccaga tgaaccaaat tttatttgga aaaatcggaa
    58261 tgcagtaatt gatatgaatt gcatcattga ggcagagctt ccagatatta tgccgaacaa
    58321 gcgatacgaa acttacacga ttcagggccg aagcggagaa ttgacggaga cgtttgatga
    58381 ctatgaacct tttgatttgg aaatagaagg gatcactatt ccacattcga aactgagaga
    58441 agtcaaacga tggctcactg gtaaaagcca attaatcaca cataatgatc cagacaagta
    58501 tctagaggct atctgcagca tggataaaga agtccctttc gaaaacgagt ggggcttctt
    58561 ttatacattc gatgttactt ttcgttgtca gccactcaaa agaaaaatcg gtgaagcacc
    58621 taaaattttt cattctagtg ttatggatat ttttgatcat ggtgacgaaa ttgcacatcc
    58681 ctatctggaa atagaatcga acggaggaga tataacgatc gctgtcggtg ataaaagcct
    58741 aacaatactt aatacgttag ctgcaacaat cgtggttgat actcagcttg gaaaagcaat
    58801 acaagaaggt ttgaatttat ttacgcgtgg tgattggcca gtattgcagc ctgaatggaa
    58861 tcaggtaaaa atatccggaa aatttaaaga agtgcggttt tggaatagga gtgtgtatct
    58921 gtgacgcaag aatttattta tgcttataaa aaaatgccag atgatttgag cgtgaacgga
    58981 gcgtcgttag ttgattggga agatttgcca gaaatcaatc gtgtgctgaa tggccaatat
    59041 cgtttttatg gtaactattc aagaagcgga cagtatcgct cttatttaaa aaaaggaaat
    59101 tttataaaag cgaaggtacc agatggatca tggcaatatt ttgaaattta taatgtcaaa
    59161 aagaatttga cctccgtttc tgtaacagct cggcatattg gttttatggc caataagaac
    59221 tttattgtca agtcgtttac ggataacggc aatgggtctc agatcatgac caatcttaaa
    59281 aacagtttgg catttgatca aaaatttaac tatctttcga atgtagggac aacgcaccaa
    59341 tttacagcaa gacaagtcgc gccagttgaa gcaatcattg gttcaaataa cggtaatcaa
    59401 aatttgactg gcgtgactag cgcggaactt gatatggata actatgattt gaaactggta
    59461 aaacagattg gatcagataa tggttttcgg attgattttg gcatcaattt agaagcaatc
    59521 gaagaagaaa ttgatgaaga atcaattgtc aatagtttgt atttggttgg cggtgttcct
    59581 gataacgatt atgatgaaga caaagaacct atcgaatatg gttatttaga aattgatgga
    59641 gtaaccaacg agaatcgacg tattgccaaa cgtgaaaacg gagattgtaa aacagttgat
    59701 gaattgatca aatggggcaa aaccttattt gacaatgatc gtatccatga gccaaaagct
    59761 actcatacag tcagtatggt ggcgttggaa cacacactcg aatacggtga catgtatcaa
    59821 gagctagcgt ctttacactt tggtgatgtc gctcatgtta gggcgaaaga gctagatatc
    59881 gagataaaag agcgtatggt cgaatacact tatttcccaa cgcttgggaa atataaggat
    59941 attgttttag gtaatgattt atcactgtac acttcggcag taaatactca agcacaagaa
    60001 ttgaaaaaga aaatcgacaa tcgaacagaa acgttagttc aaaatgtgtt gaatgcgacg
    60061 gcatggatca ctggtaattc tggtgggcat gtcgtttttc gtccagaaaa agctccatca
    60121 gagattctta tcatggatac tccaaatgtg gcgaatgcaa agcgagtgtg gcgttggaat
    60181 ttaaatggct taggttattc agacaacgga gtaaatggcc cgttcggtat tgctatcaca
    60241 tcaaaaggag aaattgttgc tgacttcatc aaagtaggaa caataaatgc agaagtattc
    60301 gaaacttcct ttaatgctta tggtgatgtg ttaaaacttg taaaaggcac gcttcaaatt
    60361 tggaatgaaa acaaaaaaat catggagcta accaaaaaag gtatggaatt ctggaataga
    60421 aaagaatcca ttggaacgat cggaacaaca gattcagcag gaaatccatt tccaaatgct
    60481 gtaacaccta cacctcttga ggaaaattct ttggtaatac gtacaaatgg cgatggaaaa
    60541 tacattttaa tttctccaac tgcagaaaaa ggatttgtgt tattaggaaa cggaaaagca
    60601 tattatttcg gtgatttaga tattcaggga aaacttactg ttagaggtaa agaagtgata
    60661 ccaggacaaa atggcggtcc atctggcgga ggagaaacac ctggtgggta tcctgatgaa
    60721 ttaaaaacag atgcagaaaa gagagcttgg agaatatacg atattttgtg taataacgga
    60781 tttacgaaac aatctgcatg tggaatattg gggaacattc aacaagaaac aggaggaact
    60841 tttgatcctg atactgttca aataggtgga ccagcatacg gattagttca gtgggatggt
    60901 tcttcatatc ctcttgttgg tccagcaaca tgggatggaa aagtttatgt tcaaaactta
    60961 tttaatgctg caggtattaa agaaccaata acgagcttag atgcacaagt tcgattgctt
    61021 atttggacat ttacaaatgg acaatggatg ggcgtagtac aacctacgac ggttgatgga
    61081 tttaaggcct gtactgatcc aagacaagca gcatatgctt ttgaacgaaa ctatgaacgt
    61141 ccggcagcga cacatcctga acgtcaagat tatgcagtta actggtataa caaatttaaa
    61201 gatttaaaac caggaggagc tactggagaa gcaggactaa aacatttaga gtctttgatt
    61261 ggacaacgaa ttggcaatgg tcagtgttat ggcttgtctg cagaatattc aggatatctg
    61321 ggtggttgtg gcatgggtgc tggaacaaaa tatggtttaa ctcatgtgat aggaaatact
    61381 tctgcagcat ctgatattgg tattgcctat gattggtctg ctgttggttg gaaagtgatt
    61441 caaaacccta gatatgatca attagtagtt ggtgcaatta ttaattgggc aagaggtgga
    61501 caagtgggtt catggtttgc agacggaact tatggacata ctggcgttat ccgaggctta
    61561 gctaatggtc gtatgcaaac ttatgaacaa aacacagagt taggtatgat ttgtggaaag
    61621 ttagatcgcc agtattatag tgcaagtgca atttcttcca ttgtcatacc accaaaatag
    61681 ggaggtgatt taatggcaaa gtggaatgtc atattaagta caacagaacc ctataattac
    61741 gtggggatga ttcaagttcg acaaggcaat aaaaacactg aagttatgga agctacaatt
    61801 gtcgaaaatg gtcttccata tgatttatca gaatgtaagg tatattttga atcagttgtt
    61861 ggcggaaaat atccagtcca attagaaaca agaattgtgg atgctaaaaa agggaaaatc
    61921 aa
//
LOCUS       DASHWH010000006        59518 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.006, whole genome shotgun sequence.
ACCESSION   DASHWH010000006 DASHWH010000000
VERSION     DASHWH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.006"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(451..1761)
                     /locus_tag="U2R41_000409"
     CDS             complement(451..1761)
                     /locus_tag="U2R41_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEM8887847.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="U2R41_000410"
     CDS             complement(1773..3191)
                     /locus_tag="U2R41_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8887848.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="U2R41_000411"
     CDS             complement(3431..4999)
                     /locus_tag="U2R41_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM8887849.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="U2R41_000412"
     CDS             complement(5003..5413)
                     /locus_tag="U2R41_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEM8887850.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="U2R41_000413"
     CDS             complement(5410..6333)
                     /locus_tag="U2R41_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEM8887851.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="U2R41_000414"
     CDS             complement(6330..7208)
                     /locus_tag="U2R41_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEM8887852.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     LALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="U2R41_000415"
     CDS             complement(7195..8532)
                     /locus_tag="U2R41_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEM8887853.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="U2R41_000416"
     CDS             complement(8535..9638)
                     /locus_tag="U2R41_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8887854.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="U2R41_000417"
     CDS             complement(9712..11220)
                     /locus_tag="U2R41_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8887855.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="U2R41_000418"
     CDS             complement(11186..11878)
                     /locus_tag="U2R41_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8887856.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="U2R41_000419"
     CDS             complement(11900..12874)
                     /locus_tag="U2R41_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8887857.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="U2R41_000420"
     CDS             13174..14046
                     /locus_tag="U2R41_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8887858.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="U2R41_000421"
     CDS             complement(14504..16702)
                     /locus_tag="U2R41_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887859.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="U2R41_000422"
     CDS             16934..17926
                     /locus_tag="U2R41_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8887860.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="U2R41_000423"
     CDS             17986..19407
                     /locus_tag="U2R41_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEM8887861.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20917
                     /locus_tag="U2R41_000424"
     CDS             19400..20917
                     /locus_tag="U2R41_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEM8887862.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERK
                     GHWMDFNAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20956..22350
                     /gene="uxaC"
                     /locus_tag="U2R41_000425"
     CDS             20956..22350
                     /gene="uxaC"
                     /locus_tag="U2R41_000425"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEM8887863.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22509..23192
                     /locus_tag="U2R41_000426"
     CDS             22509..23192
                     /locus_tag="U2R41_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEM8887864.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23194..24030
                     /locus_tag="U2R41_000427"
     CDS             23194..24030
                     /locus_tag="U2R41_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM8887865.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24041..25795
                     /locus_tag="U2R41_000428"
     CDS             24041..25795
                     /locus_tag="U2R41_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887866.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26071..26412)
                     /locus_tag="U2R41_000429"
     CDS             complement(26071..26412)
                     /locus_tag="U2R41_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8887867.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26480..28651)
                     /locus_tag="U2R41_000430"
     CDS             complement(26480..28651)
                     /locus_tag="U2R41_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM8887868.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28820..29719
                     /locus_tag="U2R41_000431"
     CDS             28820..29719
                     /locus_tag="U2R41_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8887869.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29790..30647)
                     /locus_tag="U2R41_000432"
     CDS             complement(29790..30647)
                     /locus_tag="U2R41_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8887870.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30632..33334)
                     /locus_tag="U2R41_000433"
     CDS             complement(30632..33334)
                     /locus_tag="U2R41_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEM8887871.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33347..34636)
                     /locus_tag="U2R41_000434"
     CDS             complement(33347..34636)
                     /locus_tag="U2R41_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEM8887872.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34786..35832
                     /locus_tag="U2R41_000435"
     CDS             34786..35832
                     /locus_tag="U2R41_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8887873.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35834..37987
                     /locus_tag="U2R41_000436"
     CDS             35834..37987
                     /locus_tag="U2R41_000436"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEM8887874.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38460..39911)
                     /locus_tag="U2R41_000437"
     CDS             complement(38460..39911)
                     /locus_tag="U2R41_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8887875.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39908..41632)
                     /locus_tag="U2R41_000438"
     CDS             complement(39908..41632)
                     /locus_tag="U2R41_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8887876.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKGLSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41625..42239)
                     /locus_tag="U2R41_000439"
     CDS             complement(41625..42239)
                     /locus_tag="U2R41_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8887877.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42584..44041)
                     /locus_tag="U2R41_000440"
     CDS             complement(42584..44041)
                     /locus_tag="U2R41_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8887878.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44082..45002)
                     /locus_tag="U2R41_000441"
     CDS             complement(44082..45002)
                     /locus_tag="U2R41_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8887879.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45014..45961)
                     /locus_tag="U2R41_000442"
     CDS             complement(45014..45961)
                     /locus_tag="U2R41_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8887880.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46440..47159)
                     /locus_tag="U2R41_000443"
     CDS             complement(46440..47159)
                     /locus_tag="U2R41_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM8887881.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47208..48854)
                     /locus_tag="U2R41_000444"
     CDS             complement(47208..48854)
                     /locus_tag="U2R41_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8887882.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49033..49623
                     /locus_tag="U2R41_000445"
     CDS             49033..49623
                     /locus_tag="U2R41_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8887883.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49715..51220
                     /locus_tag="U2R41_000446"
     CDS             49715..51220
                     /locus_tag="U2R41_000446"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEM8887884.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51304..52656)
                     /gene="celB"
                     /locus_tag="U2R41_000447"
     CDS             complement(51304..52656)
                     /gene="celB"
                     /locus_tag="U2R41_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM8887885.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52656..53174)
                     /locus_tag="U2R41_000448"
     CDS             complement(52656..53174)
                     /locus_tag="U2R41_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887886.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53190..53516)
                     /locus_tag="U2R41_000449"
     CDS             complement(53190..53516)
                     /locus_tag="U2R41_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEM8887887.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53529..55508)
                     /locus_tag="U2R41_000450"
     CDS             complement(53529..55508)
                     /locus_tag="U2R41_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8887888.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55529..55843)
                     /locus_tag="U2R41_000451"
     CDS             complement(55529..55843)
                     /locus_tag="U2R41_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEM8887889.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            56011..56304
                     /locus_tag="U2R41_000452"
     CDS             56011..56304
                     /locus_tag="U2R41_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEM8887890.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56382..57494)
                     /locus_tag="U2R41_000453"
     CDS             complement(56382..57494)
                     /locus_tag="U2R41_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8887891.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57881..58060
                     /locus_tag="U2R41_000454"
     CDS             57881..58060
                     /locus_tag="U2R41_000454"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887892.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58254..58424
                     /locus_tag="U2R41_000455"
     CDS             58254..58424
                     /locus_tag="U2R41_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8887893.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58848..>59518)
                     /locus_tag="U2R41_000456"
     CDS             complement(58848..>59518)
                     /locus_tag="U2R41_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8887894.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
BASE COUNT        17805 a        12152 c        10405 g        19156 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctagtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgatctt gtagctgcct ctgctcttgc
    20641 ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata
    20701 tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat
    20761 ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt
    20821 tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg
    20881 agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag
    20941 aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa
    21001 aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat
    21061 ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc
    21121 gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga
    21181 aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga
    21241 aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg
    21301 ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct
    21361 gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat
    21421 gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag
    21481 gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca
    21541 tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca agaactgata
    21601 gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac
    21661 gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa
    21721 aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca
    21781 ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat
    21841 gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat
    21901 ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa
    21961 acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt
    22021 ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga
    22081 tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt
    22141 agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact
    22201 agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga
    22261 gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat
    22321 gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa
    22381 acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa
    22441 tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa
    22501 gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa
    22561 acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc
    22621 agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt
    22681 tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata
    22741 acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc
    22801 tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg
    22861 tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc
    22921 caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa
    22981 gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc
    23041 ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc
    23101 cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc
    23161 ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt
    23221 gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac
    23281 ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac
    23341 atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt
    23401 tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca
    23461 ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct
    23521 acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat
    23581 cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact
    23641 gttgctgttg aagcagggat caatcatcca atctataact atcagttagc taaacgttta
    23701 atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat
    23761 aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt
    23821 tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta
    23881 cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt
    23941 cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa
    24001 cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa
    24061 aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa
    24121 ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt
    24181 aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt
    24241 gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac
    24301 gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag
    24361 agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc
    24421 tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca
    24481 agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct
    24541 ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt
    24601 tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga
    24661 agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg
    24721 gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg
    24781 attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat
    24841 atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc
    24901 cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat
    24961 gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact
    25021 gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg
    25081 aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga
    25141 tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc
    25201 tggaaaatct acactcctta acttattgat tggatttatc cagccgactt caggaaaaat
    25261 ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc
    25321 cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg
    25381 cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc
    25441 actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct
    25501 gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat
    25561 cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca
    25621 agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac
    25681 gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac
    25741 ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa
    25801 aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc
    25861 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
    25921 ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca
    25981 agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt
    26041 tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt
    26101 catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa
    26161 gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc
    26221 ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg
    26281 gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt
    26341 caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta
    26401 gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg
    26461 ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt
    26521 caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg
    26581 cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc
    26641 ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta
    26701 cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt
    26761 tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac
    26821 ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt
    26881 ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag
    26941 gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat
    27001 cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg
    27061 ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg
    27121 caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa
    27181 gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat
    27241 catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt
    27301 gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat
    27361 cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg
    27421 ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag
    27481 caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac
    27541 ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg
    27601 ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta
    27661 aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt
    27721 taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat
    27781 tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt
    27841 cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg
    27901 cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag
    27961 tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt
    28021 cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt
    28081 cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat
    28141 caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc
    28201 ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt
    28261 tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt
    28321 ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc
    28381 cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg
    28441 tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga
    28501 tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc
    28561 atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt
    28621 ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa
    28681 tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat
    28741 gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt
    28801 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
    28861 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
    28921 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
    28981 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
    29041 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
    29101 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
    29161 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
    29221 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
    29281 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
    29341 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
    29401 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
    29461 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
    29521 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
    29581 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
    29641 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
    29701 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
    29761 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
    29821 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
    29881 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
    29941 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
    30001 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
    30061 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
    30121 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
    30181 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
    30241 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
    30301 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
    30361 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
    30421 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
    30481 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
    30541 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
    30601 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
    30661 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
    30721 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
    30781 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
    30841 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
    30901 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
    30961 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
    31021 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
    31081 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
    31141 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
    31201 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
    31261 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
    31321 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
    31381 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
    31441 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
    31501 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
    31561 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
    31621 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
    31681 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
    31741 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
    31801 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
    31861 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
    31921 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
    31981 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
    32041 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
    32101 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
    32161 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
    32221 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
    32281 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
    32341 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
    32401 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
    32461 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
    32521 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
    32581 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
    32641 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
    32701 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
    32761 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
    32821 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
    32881 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
    32941 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
    33001 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
    33061 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
    33121 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
    33181 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
    33241 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
    33301 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac ttttctactc
    33361 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
    33421 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
    33481 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
    33541 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
    33601 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
    33661 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
    33721 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
    33781 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
    33841 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
    33901 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
    33961 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
    34021 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
    34081 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
    34141 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
    34201 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
    34261 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
    34321 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
    34381 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
    34441 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
    34501 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
    34561 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
    34621 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
    34681 ttcatacagc caattatgtc tacgctttaa aaaaatatct ttttatttac tttttcatcc
    34741 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
    34801 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
    34861 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
    34921 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
    34981 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
    35041 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
    35101 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
    35161 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
    35221 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
    35281 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
    35341 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
    35401 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
    35461 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
    35521 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
    35581 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
    35641 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
    35701 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
    35761 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
    35821 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
    35881 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
    35941 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
    36001 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
    36061 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
    36121 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
    36181 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
    36241 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
    36301 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
    36361 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
    36421 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
    36481 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
    36541 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
    36601 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
    36661 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
    36721 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
    36781 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
    36841 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
    36901 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
    36961 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
    37021 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
    37081 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
    37141 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
    37201 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
    37261 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
    37321 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
    37381 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
    37441 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
    37501 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
    37561 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
    37621 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
    37681 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
    37741 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
    37801 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
    37861 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
    37921 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
    37981 taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    38041 aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa
    38101 caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg
    38161 gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    38221 acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt
    38281 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
    38341 tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc
    38401 ccaaaattag atttctagac aattttttat aagttatttc attacattgg ctttttcttt
    38461 tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat
    38521 aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt
    38581 tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct
    38641 gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc
    38701 aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg
    38761 attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac
    38821 agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt
    38881 aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa
    38941 ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca
    39001 gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa
    39061 aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta
    39121 tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt
    39181 ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt
    39241 tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat
    39301 acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta
    39361 ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca
    39421 cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga
    39481 ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac
    39541 cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa
    39601 taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat
    39661 aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca
    39721 tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca
    39781 gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa
    39841 tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga
    39901 tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct
    39961 caagcgacat ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca
    40021 gaccgataga accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta
    40081 gccgtttttc agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt
    40141 cctgcaagac tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa
    40201 aataattttc aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag
    40261 gatcgatcat aaaatgatag gctactcgat taggataacg catttgatac aaatagacat
    40321 atttttcgca aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt
    40381 ttcgaagcaa agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga
    40441 ttgcatacat tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt
    40501 gaagcgcccg catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa
    40561 actgattgat gctatccaac atttcgttga taccgattga aagtcccttc agttcatatt
    40621 ccgtgtttgt tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct
    40681 gcatgacgat agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca
    40741 gtatattgtc taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga
    40801 tttttacact ttttttatct agtacagcta atatacgata atcatcagac agttcttggt
    40861 gttgaacaaa attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac
    40921 tctctgtgat gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga
    40981 atgtgtatag tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg
    41041 ttagattttc taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg
    41101 actcagctcc tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg
    41161 tccccttttt tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt
    41221 gattcaacac gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt
    41281 gatttgggaa agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat
    41341 actgatctat gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta
    41401 caagctggtt tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc
    41461 tttgaacggc atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga
    41521 tactaaaaat aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca
    41581 acagacgatt tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc
    41641 aactgtttat ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa
    41701 agaccaatga caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata
    41761 ccgctaccaa taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt
    41821 agctttagca gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga
    41881 taagtagcaa ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg
    41941 agccctttga tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa
    42001 aataaactaa atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt
    42061 tttattgtga tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc
    42121 catgccgcac ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag
    42181 gttagtttta taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata
    42241 tcttctcctc caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc
    42301 agcaccttga cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt
    42361 tttgtgagcg aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat
    42421 agccgcttag tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca
    42481 tatatttagc ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt
    42541 tgtgacacgt cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact
    42601 catcatattg tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt
    42661 ctttcaattt cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat
    42721 attgattcat gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc
    42781 tgaatcctaa aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca
    42841 tgtcatccgt aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag
    42901 acatatgtgt atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga
    42961 ctttttccca tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac
    43021 tgttaagcag ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga
    43081 caaaatttgc catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga
    43141 caagttcttg ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg
    43201 tttctccacg cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag
    43261 cgtcacttgg aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat
    43321 acgtttcatt atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa
    43381 atggtaacgt attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa
    43441 tggcaaaagc tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat
    43501 cagcgtaaga ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa
    43561 aagtaagtac ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat
    43621 tgaccagatt tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact
    43681 taggcgctaa ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt
    43741 tcgcaaaggc gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt
    43801 aatcgcccca gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt
    43861 tgtttgctac ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct
    43921 gcaaagtgtc cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg
    43981 ctaatgttcc caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca
    44041 tttttcttcc tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg
    44101 attgttagac ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac
    44161 gtcgagatga ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct
    44221 gtcaattggc ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc
    44281 atcaaaagat actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac
    44341 cagtcattcc agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct
    44401 aacggtaaaa cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat
    44461 tcaataatcg aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag
    44521 ggactgacag ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt
    44581 gtcataacaa gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa
    44641 acggtaaaaa aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa
    44701 gtagccgtgc tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct
    44761 tggaaaattt tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt
    44821 tctggccaaa aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg
    44881 ataacaaata taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag
    44941 aaaaaattag ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc
    45001 atttttttcc tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg
    45061 tgatcatcaa caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac
    45121 caaaatctcc tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat
    45181 catataatgc accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa
    45241 tgtttcctac ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca
    45301 catgtttgat ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg
    45361 gatcgattcc cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg
    45421 taccaatgaa caccaagata aacggccaat atttcggttc attataccaa ttgacagctt
    45481 ctcctccatg tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt
    45541 aaacaaagta actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat
    45601 aaactttaac gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga
    45661 tcagcagatt caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa
    45721 atgcttgatc cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc
    45781 tcaagctagc caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg
    45841 ccattacagg tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga
    45901 caagtgcttt ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca
    45961 tctaaaaacc cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata
    46021 aataaacaaa gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt
    46081 tggcctgagt attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga
    46141 gctaataccg agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt
    46201 tggcctgagt attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat
    46261 ggggactgta acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag
    46321 tagcttctac ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca
    46381 agtctgcttt tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat
    46441 tatttttctc tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc
    46501 aaatctttag gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt
    46561 cctactgctt tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg
    46621 ataattgaag tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc
    46681 tgctcatcgc catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt
    46741 ataccgctaa cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga
    46801 tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg
    46861 ccgactggaa aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga
    46921 tcatgattat cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa
    46981 tctttttgat acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact
    47041 tgcgctttat cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat
    47101 cctttgactt ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg
    47161 cgtttccccc atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt
    47221 aatttatgtc gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc
    47281 aaagctaaat aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga
    47341 gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa
    47401 gccaaaagca taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt
    47461 ggtttaaagc gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat
    47521 ccgatcattg tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga
    47581 agaaggagtt tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac
    47641 attccttgca gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt
    47701 gcttggatca gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga
    47761 taggcaagta acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa
    47821 tttccgcttg tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc
    47881 aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta
    47941 tctggattgg cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg
    48001 gatgcagaca tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct
    48061 gaacccacaa taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt
    48121 gcaatattta ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc
    48181 ttcaaaaaat acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt
    48241 gattgcgtga ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc
    48301 aatagcatat aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc
    48361 atttcttgat tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca
    48421 gcgatactca aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct
    48481 agccaaggag aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc
    48541 tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt
    48601 gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca
    48661 tatatattgt atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa
    48721 gcataccagg gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc
    48781 atccaagcag agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt
    48841 ctttgattat ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg
    48901 gtaaaacaga aactggcaat cgattttata taacttttat aagaacaatt gtttttttga
    48961 attcggtttc gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta
    49021 taggagatat caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc
    49081 tttatcacag ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg
    49141 ggcgccatcg gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg
    49201 agcatcaatc ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt
    49261 aagactgtag cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa
    49321 atgatctcct cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt
    49381 tacacattgt acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc
    49441 cacaaatttt ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc
    49501 tttttgaatt attatgtttt tggattgttg gagacactat tcgttctcct atctattaca
    49561 gtcagttcat tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa
    49621 taaagctgga ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata
    49681 aaccaagaaa ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg
    49741 tcatttacta ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct
    49801 tgatgtacca gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc
    49861 ttctactttg tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat
    49921 cgaacagggt attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat
    49981 catcgtaacg gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc
    50041 gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta
    50101 ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat
    50161 gaatacaacg ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt
    50221 agatttatat cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga
    50281 agtttcttca caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat
    50341 cttcttgaat atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta
    50401 tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag
    50461 tgactatttt gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta
    50521 tggtcgttcg gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt
    50581 tactttatct tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct
    50641 ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg
    50701 tgcctccagt caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga
    50761 tcaattgatc caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag
    50821 cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt
    50881 attaggcagc ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc
    50941 agaaacagct gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa
    51001 aatcgcggaa gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat
    51061 ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt
    51121 tattgcggga aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga
    51181 aggggattat gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg
    51241 tttcccattt ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag
    51301 aaatcatttt aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa
    51361 tttattatca tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat
    51421 gactgctaac acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac
    51481 tggtgaaggc cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa
    51541 ataagcgagt gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt
    51601 gtagacgatt ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag
    51661 tgaggctttt cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat
    51721 caatcctaaa gtcgcacctg atccaccaac ggttacataa gaactgttga attctcctgc
    51781 caacggataa tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa
    51841 gaaagaagtg atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa
    51901 ataaatcacc atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat
    51961 agcaatcatt ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac
    52021 cacaaacgca atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg
    52081 aacaacatct ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac
    52141 cgcaataatc gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa
    52201 agagcccatc ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct
    52261 gattttacca tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag
    52321 taatgccccc gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa
    52381 ttcataagca aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac
    52441 gatcatgtac aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg
    52501 gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat
    52561 agaagccatc cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat
    52621 aggtcccatc aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat
    52681 aacgcttttt catattgttt aatacggata tactgttggt cttggttgag tgaaattttc
    52741 tttaaacgat actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca
    52801 aataactgtc ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta
    52861 aacaacactg gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc
    52921 catggaacgt tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa
    52981 atagctaaaa tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga
    53041 atcatccatt gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa
    53101 cgattgaaat acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt
    53161 actgtatcct gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt
    53221 tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg
    53281 ataatttcca tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac
    53341 tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac
    53401 atctctttac gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg
    53461 aaaatgatat caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt
    53521 tttcctcctt attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca
    53581 gctaagattt cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg
    53641 accgtaattc cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct
    53701 tgatattgat cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt
    53761 ccaactggaa cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata
    53821 ttttcacgtt gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg
    53881 ctcagctttt gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc
    53941 ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat
    54001 gctggtatcg gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt
    54061 ttacgaacat catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac
    54121 attgtcgaaa gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg
    54181 atctcataat agccttttac acttaccact gtaatatttt taccgaattc actgactacg
    54241 cgattcttta atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt
    54301 ttatgcgcag ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat
    54361 tcatcttcat tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact
    54421 gcgtcaaaga ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga
    54481 atacctcggc tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc
    54541 tggagatcct ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct
    54601 aattcctttg atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga
    54661 tgtaatgtca aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata
    54721 cgttgaacga tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg
    54781 cctaattcct tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa
    54841 gtcagattct gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga
    54901 atgataatga ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat
    54961 tctttcaatg aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga
    55021 tttctttctt ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa
    55081 tatggaatca atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata
    55141 taaagctctt ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc
    55201 aatatatact tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca
    55261 ggtactattt cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt
    55321 tggtagccat aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg
    55381 atccgctgca agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt
    55441 tgactggtga catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc
    55501 tgtttcatta tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact
    55561 tctggatgcc tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc
    55621 caacagattc tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca
    55681 gaaataaatc aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat
    55741 ctacgagtac ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg
    55801 aggatgacat tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt
    55861 tatttttata aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta
    55921 gcattgcgga aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact
    55981 agtgatagaa ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca
    56041 agtacaaatg gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa
    56101 gcgattcgtg cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca
    56161 gaatctcttt tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa
    56221 tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc
    56281 gaacgtttca ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca
    56341 tgtgtatctt cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt
    56401 ttttctctta tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat
    56461 accccaagga tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg
    56521 gcagtgattg ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa
    56581 acacctaaag ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata
    56641 ccaataaata aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg
    56701 aaaccaatcg acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac
    56761 atcaatcgct gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca
    56821 tagatgagca tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac
    56881 aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa
    56941 aaaaacaaaa ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta
    57001 ggtaacgaat aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg
    57061 ggatcactag cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc
    57121 ataactgacc atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt
    57181 gctaaaccta tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc
    57241 aacatacaga ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt
    57301 ataatgataa aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa
    57361 atatacatgc acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac
    57421 ggatctagtt gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta
    57481 ttttttacag tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat
    57541 tttcttgtca actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta
    57601 tgattaaatc accacaaaat aaacaactga gagaacgata tgctttatga atggttctat
    57661 caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac
    57721 cttacagaag aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg
    57781 tagaaattgt ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa
    57841 gaagaaggaa accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct
    57901 aaaaaagcag cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt
    57961 tatccgtcca aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac
    58021 tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat
    58081 tcgcacattg aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg
    58141 agtgttgagg gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt
    58201 tccgcggatg gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc
    58261 gattaacaaa ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag
    58321 acatgaacgc cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga
    58381 ttgatcgatt acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt
    58441 ggttgaagct ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca
    58501 ttaaaactag agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt
    58561 attacccaat aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc
    58621 gcagtgtttt tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc
    58681 tcaacgcagc acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc
    58741 gatactaata tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg
    58801 tactagttga ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt
    58861 ttctttctaa attttatact gatgaaaaca agtagcacac ccaatattga caatataaac
    58921 tggctaatga tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg
    58981 tttcctctta tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc
    59041 acttcttgtt ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt
    59101 ggcgcttgag tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt
    59161 ccatctgctc ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca
    59221 aaaacagctc cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg
    59281 cctggtgtaa gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc
    59341 tcaaactcga ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc
    59401 tgatacactc ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga
    59461 cctgactgca gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcagga
//
LOCUS       DASHWH010000007        59391 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.007, whole genome shotgun sequence.
ACCESSION   DASHWH010000007 DASHWH010000000
VERSION     DASHWH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.007"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(59..187)
                     /locus_tag="U2R41_000457"
     CDS             complement(59..187)
                     /locus_tag="U2R41_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM8887895.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U2R41_000458"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U2R41_000458"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM8887896.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="U2R41_000459"
     CDS             complement(1674..2729)
                     /locus_tag="U2R41_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM8887897.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U2R41_000460"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U2R41_000460"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEM8887898.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U2R41_000461"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U2R41_000461"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEM8887899.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U2R41_000462"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U2R41_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEM8887900.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U2R41_000463"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U2R41_000463"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEM8887901.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U2R41_000464"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U2R41_000464"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEM8887902.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="U2R41_000465"
     CDS             10491..12050
                     /locus_tag="U2R41_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEM8887903.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="U2R41_000466"
     CDS             complement(12161..14092)
                     /locus_tag="U2R41_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8887904.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U2R41_000467"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U2R41_000467"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEM8887905.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="U2R41_000468"
     CDS             complement(15021..15773)
                     /locus_tag="U2R41_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8887906.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="U2R41_000469"
     CDS             complement(15932..16141)
                     /locus_tag="U2R41_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887907.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="U2R41_000470"
     CDS             complement(16152..17480)
                     /locus_tag="U2R41_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887908.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="U2R41_000471"
     CDS             17616..17771
                     /locus_tag="U2R41_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887909.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="U2R41_000472"
     CDS             complement(17845..18432)
                     /locus_tag="U2R41_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEM8887910.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U2R41_000473"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U2R41_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEM8887911.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="U2R41_000474"
     CDS             complement(20012..21301)
                     /locus_tag="U2R41_000474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEM8887912.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="U2R41_000475"
     CDS             complement(21455..22387)
                     /locus_tag="U2R41_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887913.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="U2R41_000476"
     CDS             complement(22544..23203)
                     /locus_tag="U2R41_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM8887914.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U2R41_000477"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U2R41_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEM8887915.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="U2R41_000478"
     CDS             complement(24244..24534)
                     /locus_tag="U2R41_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887916.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="U2R41_000479"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="U2R41_000479"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEM8887917.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="U2R41_000480"
     CDS             complement(26821..28176)
                     /locus_tag="U2R41_000480"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEM8887918.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="U2R41_000481"
     CDS             complement(28200..29369)
                     /locus_tag="U2R41_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887919.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="U2R41_000482"
     CDS             complement(29366..30253)
                     /locus_tag="U2R41_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEM8887920.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="U2R41_000483"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="U2R41_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEM8887921.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="U2R41_000484"
     CDS             34320..34841
                     /locus_tag="U2R41_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM8887922.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="U2R41_000485"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="U2R41_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="U2R41_000486"
     CDS             complement(36284..36940)
                     /locus_tag="U2R41_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887923.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="U2R41_000487"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="U2R41_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEM8887924.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="U2R41_000488"
     CDS             complement(38477..39475)
                     /locus_tag="U2R41_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEM8887925.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="U2R41_000489"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="U2R41_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEM8887926.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="U2R41_000490"
     CDS             complement(40491..41222)
                     /locus_tag="U2R41_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEM8887927.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="U2R41_000491"
     CDS             complement(41226..42491)
                     /locus_tag="U2R41_000491"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEM8887928.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="U2R41_000492"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="U2R41_000492"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEM8887929.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="U2R41_000493"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="U2R41_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEM8887930.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="U2R41_000494"
     CDS             47833..49335
                     /locus_tag="U2R41_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEM8887931.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="U2R41_000495"
     CDS             49337..50074
                     /locus_tag="U2R41_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8887932.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="U2R41_000496"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="U2R41_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEM8887933.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="U2R41_000497"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="U2R41_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEM8887934.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="U2R41_000498"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="U2R41_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEM8887935.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="U2R41_000499"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="U2R41_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEM8887936.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="U2R41_000500"
     CDS             complement(55574..56740)
                     /locus_tag="U2R41_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEM8887937.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="U2R41_000501"
     CDS             complement(56756..56983)
                     /locus_tag="U2R41_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEM8887938.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="U2R41_000502"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="U2R41_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="U2R41_000503"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="U2R41_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16403 a        12847 c        10313 g        19828 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttgaacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcatttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt t
//
LOCUS       DASHWH010000008        56802 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.008, whole genome shotgun sequence.
ACCESSION   DASHWH010000008 DASHWH010000000
VERSION     DASHWH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.008"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(106..1404)
                     /gene="eno"
                     /locus_tag="U2R41_000504"
     CDS             complement(106..1404)
                     /gene="eno"
                     /locus_tag="U2R41_000504"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEM8887939.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1520..2275)
                     /locus_tag="U2R41_000505"
     CDS             complement(1520..2275)
                     /locus_tag="U2R41_000505"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEM8887940.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2379..3569)
                     /locus_tag="U2R41_000506"
     CDS             complement(2379..3569)
                     /locus_tag="U2R41_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEM8887941.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3679..4680)
                     /gene="gap"
                     /locus_tag="U2R41_000507"
     CDS             complement(3679..4680)
                     /gene="gap"
                     /locus_tag="U2R41_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM8887942.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4721..5812)
                     /locus_tag="U2R41_000508"
     CDS             complement(4721..5812)
                     /locus_tag="U2R41_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEM8887943.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5961..7295)
                     /gene="rpoN"
                     /locus_tag="U2R41_000509"
     CDS             complement(5961..7295)
                     /gene="rpoN"
                     /locus_tag="U2R41_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEM8887944.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7355..7426
                     /locus_tag="U2R41_000510"
     tRNA            7355..7426
                     /locus_tag="U2R41_000510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7388..7390,aa:Arg,seq:ccg)
     gene            complement(7618..8502)
                     /locus_tag="U2R41_000511"
     CDS             complement(7618..8502)
                     /locus_tag="U2R41_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8887945.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8589..9488)
                     /locus_tag="U2R41_000512"
     CDS             complement(8589..9488)
                     /locus_tag="U2R41_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEM8887946.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9505..10011)
                     /locus_tag="U2R41_000513"
     CDS             complement(9505..10011)
                     /locus_tag="U2R41_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEM8887947.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10200..10820)
                     /locus_tag="U2R41_000514"
     CDS             complement(10200..10820)
                     /locus_tag="U2R41_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEM8887948.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10896..11333)
                     /locus_tag="U2R41_000515"
     CDS             complement(10896..11333)
                     /locus_tag="U2R41_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEM8887949.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11496..12326
                     /locus_tag="U2R41_000516"
     CDS             11496..12326
                     /locus_tag="U2R41_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEM8887950.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12310..13677
                     /locus_tag="U2R41_000517"
     CDS             12310..13677
                     /locus_tag="U2R41_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8887951.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13696..14505
                     /gene="yidA"
                     /locus_tag="U2R41_000518"
     CDS             13696..14505
                     /gene="yidA"
                     /locus_tag="U2R41_000518"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEM8887952.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14548..14991)
                     /locus_tag="U2R41_000519"
     CDS             complement(14548..14991)
                     /locus_tag="U2R41_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEM8887953.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15125..15496
                     /locus_tag="U2R41_000520"
     CDS             15125..15496
                     /locus_tag="U2R41_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8887954.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15665..16447)
                     /locus_tag="U2R41_000521"
     CDS             complement(15665..16447)
                     /locus_tag="U2R41_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEM8887955.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16444..17415)
                     /locus_tag="U2R41_000522"
     CDS             complement(16444..17415)
                     /locus_tag="U2R41_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEM8887956.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17507..18901
                     /locus_tag="U2R41_000523"
     CDS             17507..18901
                     /locus_tag="U2R41_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM8887957.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(19093..20448)
                     /locus_tag="U2R41_000524"
     CDS             complement(19093..20448)
                     /locus_tag="U2R41_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEM8887958.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(20478..21113)
                     /locus_tag="U2R41_000525"
     CDS             complement(20478..21113)
                     /locus_tag="U2R41_000525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEM8887959.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(21120..22496)
                     /locus_tag="U2R41_000526"
     CDS             complement(21120..22496)
                     /locus_tag="U2R41_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEM8887960.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(22489..24270)
                     /locus_tag="U2R41_000527"
     CDS             complement(22489..24270)
                     /locus_tag="U2R41_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEM8887961.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(24292..24603)
                     /locus_tag="U2R41_000528"
     CDS             complement(24292..24603)
                     /locus_tag="U2R41_000528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEM8887962.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(24593..25579)
                     /locus_tag="U2R41_000529"
     CDS             complement(24593..25579)
                     /locus_tag="U2R41_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEM8887963.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(25594..26178)
                     /locus_tag="U2R41_000530"
     CDS             complement(25594..26178)
                     /locus_tag="U2R41_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEM8887964.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            complement(26199..26669)
                     /locus_tag="U2R41_000531"
     CDS             complement(26199..26669)
                     /locus_tag="U2R41_000531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEM8887965.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            complement(26688..28691)
                     /locus_tag="U2R41_000532"
     CDS             complement(26688..28691)
                     /locus_tag="U2R41_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEM8887966.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            complement(28678..29010)
                     /locus_tag="U2R41_000533"
     CDS             complement(28678..29010)
                     /locus_tag="U2R41_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887967.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            29347..30066
                     /locus_tag="U2R41_000534"
     CDS             29347..30066
                     /locus_tag="U2R41_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM8887968.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            complement(30396..32294)
                     /locus_tag="U2R41_000535"
     CDS             complement(30396..32294)
                     /locus_tag="U2R41_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEM8887969.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINEKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(32455..33717)
                     /locus_tag="U2R41_000536"
     CDS             complement(32455..33717)
                     /locus_tag="U2R41_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887970.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(33710..34609)
                     /locus_tag="U2R41_000537"
     CDS             complement(33710..34609)
                     /locus_tag="U2R41_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887971.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(34754..36532)
                     /gene="uvrC"
                     /locus_tag="U2R41_000538"
     CDS             complement(34754..36532)
                     /gene="uvrC"
                     /locus_tag="U2R41_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEM8887972.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(36714..37028)
                     /gene="trxA"
                     /locus_tag="U2R41_000539"
     CDS             complement(36714..37028)
                     /gene="trxA"
                     /locus_tag="U2R41_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEM8887973.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(37115..39475)
                     /locus_tag="U2R41_000540"
     CDS             complement(37115..39475)
                     /locus_tag="U2R41_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM8887974.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(39590..40138)
                     /locus_tag="U2R41_000541"
     CDS             complement(39590..40138)
                     /locus_tag="U2R41_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEM8887975.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYITIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(40196..40633)
                     /gene="zapA"
                     /locus_tag="U2R41_000542"
     CDS             complement(40196..40633)
                     /gene="zapA"
                     /locus_tag="U2R41_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEM8887976.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            40835..41797
                     /locus_tag="U2R41_000543"
     CDS             40835..41797
                     /locus_tag="U2R41_000543"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEM8887977.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKKKWNCDKSHVTVPCSE"
     gene            complement(42130..43041)
                     /locus_tag="U2R41_000544"
     CDS             complement(42130..43041)
                     /locus_tag="U2R41_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM8887978.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43442..44020)
                     /locus_tag="U2R41_000545"
     CDS             complement(43442..44020)
                     /locus_tag="U2R41_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8887979.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(44040..44708)
                     /locus_tag="U2R41_000546"
     CDS             complement(44040..44708)
                     /locus_tag="U2R41_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8887980.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(44723..45793)
                     /locus_tag="U2R41_000547"
     CDS             complement(44723..45793)
                     /locus_tag="U2R41_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8887981.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(45964..47328)
                     /locus_tag="U2R41_000548"
     CDS             complement(45964..47328)
                     /locus_tag="U2R41_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8887982.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(47325..48884)
                     /locus_tag="U2R41_000549"
     CDS             complement(47325..48884)
                     /locus_tag="U2R41_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8887983.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(48940..51420)
                     /locus_tag="U2R41_000550"
     CDS             complement(48940..51420)
                     /locus_tag="U2R41_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887984.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(51404..51739)
                     /locus_tag="U2R41_000551"
     CDS             complement(51404..51739)
                     /locus_tag="U2R41_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887985.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(51753..52787)
                     /locus_tag="U2R41_000552"
     CDS             complement(51753..52787)
                     /locus_tag="U2R41_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM8887986.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(52877..53557)
                     /locus_tag="U2R41_000553"
     CDS             complement(52877..53557)
                     /locus_tag="U2R41_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8887987.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(53727..54686)
                     /locus_tag="U2R41_000554"
     CDS             complement(53727..54686)
                     /locus_tag="U2R41_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8887988.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(54907..56010)
                     /locus_tag="U2R41_000555"
     CDS             complement(54907..56010)
                     /locus_tag="U2R41_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM8887989.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(56232..56702)
                     /locus_tag="U2R41_000556"
     CDS             complement(56232..56702)
                     /locus_tag="U2R41_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEM8887990.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT        16019 a        12007 c         9877 g        18899 t
ORIGIN      
        1 ctctacctgc tgatatttca gcaagtagag agttcgatta cttaactaag tgatcatact
       61 cgactatctt tcaatagtcg atcatgtatc ttcattagaa aagaattatt tgttttttaa
      121 gttgtagaaa gatttcaaac ctttgtattc tgcaacttca ccaagttggt cttcgatacg
      181 tagtaattgg ttgtatttag caatacggtc agtacgtgat agagaaccag ttttgatttg
      241 gccagcattt gttgcaacag cgatatcaga gattgttgaa tcttctgttt caccagaacg
      301 gtgagataca actgctgtgt agccagcttc tttagccatt tcgatagctt cgaaagtttc
      361 tgttaatgta ccgatttggt tcactttgat aaggattgag ttagcaattc ctttttcgat
      421 accttcagat aatttttgag tgtttgttac gaacaagtcg tcaccaacta attgaacttt
      481 gtcgcctaat acgtcagtta atttcttgaa tccgtcccag tcgttttcgt ctaatccatc
      541 ttcgattgag atgattggat atttagaaac taattcttcg tagaatttga tcatttcgtc
      601 agttgttttt tcgccttcgc ctgagtcagc tagtacgtaa acaccttttt ctttgtcgta
      661 gaattctgaa gaagcagcat ccatagcaag aacaacgtct ttaccaggta catagccagc
      721 tttttcgatt gcttcgatga ttacttcaaa accttcttcg tttgaaccaa ggtttggagc
      781 aaatccacct tcgtcaccaa cagaagttgc taatccgcga gctttcaaga ttgaagctaa
      841 tgcgtggaat acttcagcac ccatacgtag agcttctttg aatgttggag cgccaacagg
      901 catgatcatg aattcttgga agtcgattga gttatcagca tgtgatccgc cgttgatgat
      961 gttcatcatt ggtgttggca atacttttgt gttgaatccg cctaggtagt ggtataaagg
     1021 tacttctagg tagtcagcag ctgcacgtgc aacagcgata gaaacaccta aaatagcgtt
     1081 cgcacctaat ttacctttgt taggagttcc gtctaaagcg atcatagctt tgtcgatagc
     1141 catttggtca cgaacatcgt agccaatgat tgcttcagca atgatgttgt ttacgttgtc
     1201 gacagcttta gttacacctt taccgccata acgagctttg tctccgtcac gtaattcaac
     1261 tgcttcgtat tcaccagttg aagcacctga aggaaccatt ccgcggccaa aagctccgct
     1321 ttctgtgtat acttctactt cgattgttgg gttaccacgt gagtctaaga tctcgcgagc
     1381 gtaaacatca gtaataattg acatgttttg tctctccttt gagtttgttt tttcctaagg
     1441 gatttccctc atatacgatt ttagttaatt ttgctcatta gtgcaaacat ttcttgcatt
     1501 tttcgcaaat tttcaacaat tattttacag cgtctaacaa tgacaaaaat gaatctgctt
     1561 gcaagcttgc gccaccgact aaagcaccgt caacattttc ttttgccatg tattcagcaa
     1621 tattttctgg ttttacagaa ccgccgtatt ggatacgaac tgcttctgat acttcttttc
     1681 cataaagttt ttcaactgtt tgacgtacaa ctccacaaat ttcgtcagcg atttgtgcgt
     1741 cagctgattt accagtaccg attgcccaga ttggttcata agcaataacc atgtttgaca
     1801 cttgttcagc tgaaagatca gctaaaccag ctgtaatttg tccttcgatc cattcagcag
     1861 ttttgcctgc ttcataggtt tctaaagatt cgccgcagca taggattggt gtcataccat
     1921 ttgcaaagat agcttttgct tttttattga tgtcttcgtc tgtttcatgg aaatattcac
     1981 gacgttctga atgtccgatg atcacataat gaacacctag atcagcaaga gctgctggtg
     2041 aagtttcacc agtgaacgca cctgagtttt cccagtagca gttttgtgct gagattttta
     2101 gttctgtacc ttcagctgat gtcattaatt cttgtaagaa taatgcagga gatccgatga
     2161 ctgaatcaac tttgtcattt gaagggattt tgttttttac tgcttcagca aattcttttg
     2221 cttctgatgc agttttgttc attttccagt tacctgcgat aataggttta cgcattgagg
     2281 aacacactcc ttaatttatt gaaaaatggc tagggagtca tgaaaactcc cttcaccttt
     2341 tgatcgatta ctcgtcagaa ttgagcaaac ttggtcgttt atttatcgtt gattgctgct
     2401 agtcctggta attctttacc ttcaagcaat tccaagcttg cgccaccacc tgttgagatg
     2461 tgtgtgaatt tgtcagcaaa tcctaattgt tctgctgctg cagctgagtc tccgccaccg
     2521 atgattgttg ttgcgccttc taagttagcg attgcttcac aaacaccgat tgtaccttta
     2581 gcaaagttac tcatttcaaa tacacccata ggtccgttcc aaacaactgt ttttgcacct
     2641 tgtaatgtat cagcaaataa ttttactgat gctgggccga tgtctaaagc catcaaacca
     2701 tcaggaatgt ctccttcgat tacttgtgtt tcaacatcat ttgaaaattc tggtgcacag
     2761 acattgtcga ttggcaagac aagtttgtcg cctgcttttt cgattaattc ttttgctagt
     2821 tccactttat cagcttctac tagagagtta ccgatcttga ttccttttgc ttcgtagaat
     2881 gtgtaagtca ttccgccacc gatcaagatt ttatctgctt taggaatcaa gttttcgatt
     2941 acgccgattt tatcagaaac ttttgcgcca ccaaggattg caatcattgg tcgttttggt
     3001 tcttctactg cttcaccgat gaacttgatt tctttttcca tcaagaatcc agcaactgtt
     3061 gggataccag ttgaagcgat acctacgtta gaagcatgtg cacggtgagc tgtaccgaaa
     3121 gcatcgttaa caaatacatc gcctaatgaa gcccagtatt tacctagttc tgtatcattt
     3181 ttgctttctt ttttaccgtc aatatcttca aatcgtgtat tttcgaatac gacaacgtcg
     3241 ccgtcattca tgttgcggat tgcttcttct aattgttctc cgcgagtttc aggtacgaat
     3301 gttacttctt tacctaataa ttcgcctaga cgttttgcaa ctggagctaa agattttcct
     3361 tctttatctt cttctgtttt tacacgtcca aggtgagaga aaaggattgc ttttccgcct
     3421 tgttctagaa cgtagttgat tgttggcaat gctgctttga tacgagtgtc gtcagtgatc
     3481 acgccgtctt tcaaaggaac gttgaagtca acacggacaa gaactttttt gtcttttaaa
     3541 tcgatatctt tcactgtttt cttagccatt ttatttcctc ctgaataaac ttattatcaa
     3601 aaaaagcgga agaagcgcga ttgcttcccc gcttttaaat gtacatgtct ataatttaca
     3661 cttgtacgtt tgtctatctt ataagttagc gaagtattct aaagtacgta ctaattgtgc
     3721 agtgtatgac atttcgttgt cgtaccaagc aacagtttta actaattgtt tgtcgccaac
     3781 tgtcattact ttagtttgtg tagcatcaaa taatgaaccg taagtcatac caacgatatc
     3841 tgaagaaacg atttcgtctg tgttgtatcc gtaagattcg tttgaagctt ctttcattgc
     3901 tgcattgatt tcgtcaacag ttacttcttt ttcaagaact gttactaatt ctgttaatga
     3961 acctgttggt acaggaacac gttgagcagc tccgtctaat ttaccgttca attcaggaat
     4021 tactaaaccg atagctttag cagcacctgt tgagttagga acgatgtttg cagcagcagc
     4081 gcgtgcacgg cggaagtcac ctttaggatg aggtccgtct agagtcattt ggtcacctgt
     4141 gtaagcgtgg atagttgtca ttaatccttc aacaacacca aatttgtcgt tcaatgtttt
     4201 agccatagga gctaaacagt tagtagtaca tgaagcacct gagataactg tttctttacc
     4261 agttaatgtt tcgtggtttg tgttataaac gattgttgga acatcgtttc cgccaggagc
     4321 agagataaca acgcgtttag caccagctgt taaatgtttt tcagctgctg atttagaagt
     4381 gaagaaacca gtacattcta gaacgatatc tacgcctagt tcgccccatg gtaattcttc
     4441 tgggttgcgg ttagctaata ctttaacttc ttttccgttt acgttgaatg aaccttcatg
     4501 aacttcaact gttccgttga aacgtccttg agttgtgtcg tattttaaca agtgtgccaa
     4561 cattttagca tctgtcaagt cgttgattgc tactacttcg attccatcta catcttggat
     4621 acgacggaat gctagacgtc caatacgtcc aaaaccatta atacctactt taactgtcat
     4681 ttaagatttc ctccttatga aaatcaaaaa aatatttatt ttaaagggtt acccctttta
     4741 aaatctcatt tgcagcagct tcatcagtga tgagccacgt ttgttttggt gcatttttca
     4801 tataagctcg aattgcttta gcttttgatt ttccgcccgc gattgccatg atgatcggga
     4861 ctttttccaa atcttgcaat tctatgccga ttctagggac cttataaacg acttcacctt
     4921 tttcattgaa aaagtagcca aaagattccg caacagcatt tgcttgtttc agcatgacta
     4981 attctttttc agtcatctta cgacgtgctg ccatgtgtaa tgcacgccca atactatgaa
     5041 taacacaatt cgcttcacta atcaagttta agacttcttg gatagatggc tcattgagta
     5101 aagaattgta agttgccaga cttaattgtt ccggaacata aagtgcacgg tggttaccgt
     5161 ttgctttagc agccattctt gcactgacag aatttgcctg tacagttatc gcttcaccaa
     5221 tcccgcctct tgcagggaca aacaggttat gccgtttctt attttccaga ttagacaatt
     5281 gttcggctac cacagccata gtagttcctc ccatcacagc gatgatattc tcgccttctg
     5341 gaagtagtcg gtctagggct tcgctcagcg cttcgccgaa tgcatcagcc actttggtct
     5401 gctcttcgct gtttcctgaa acaacgatac agcgttggtt accaaaatat tctgccaact
     5461 gttgttcggt ttgatgcgta cctaaaagct gatccatgaa gttttcaagg ctttgataaa
     5521 cttcttcgcc cttagttgtc agcgtcatac cgctttttga ggtgtcgatc aaattcagat
     5581 ttttcaaaag atctgtttct gttcgcaata ctcgttctgt gatccccata ctttccgata
     5641 aacttcgacg tccaatcggc tgcatccaat agatatttcg taaaatttta tagcgttctt
     5701 gcaacacttc gataatatct ggtgcaacag attcaatcat ttttatttca tccagcatag
     5761 cgatctcctt agttggacac tttacgtcca atgtagactt aaaacgaccc atatacgata
     5821 aaaaaagaaa ttaactattc gaatttcaca tcaataaagg gaaatctcaa cctcacgact
     5881 gtaagtctaa cagttttgct ctcgtttttc aactaaaaag actagaaaaa gtgaaatact
     5941 ttttctagtc ttttgactga ttagttatca taacgttttc tttttgatga actcgggatt
     6001 cctaataaat cacgatattt tgctactgtt cgtcttgaga tcgctatctc atcttttttc
     6061 agcaattcaa ccaatttctg gtcagacaac ggcttgtttt tgtcttcttg atcgactaat
     6121 tcctgcagct ttttcttcgc tgtatctgct gaaaggtctt ctgtctgttc tgtactattc
     6181 gtaggaattc gtgtagtgaa aaattttttt aattcaaata caccgaagtc tgtttccaaa
     6241 tatttcccgt tgactgcacg actgaccgtg gattcatgca catctatctc agttgcaact
     6301 tcctttaacg taagaggctt gattggacgt tccttgtcga taaagaaagc tttttgccgg
     6361 gaaacaatga cttgagcaac ccgcaaaatt gtatccccac gttgtaggat cgttttcttc
     6421 aaccattcga attcctgttt ccgttctttt aaataatttt cagtttcttt atctgcttgt
     6481 tgtgacattt gtttgaagta actctcttga aagcgtatat ttggctgatt ttttcggttt
     6541 gagataactt gtacattttt atcgtcgtcc actaataccc ggacatcagg aataatgtac
     6601 aattcgctgc tacggtcgaa aatccggcca gggcttggac tcaacgtttg taaatagtcg
     6661 aatatctgct gaacagcatg tagatcaaca gaaaaacgtt gtgcgatttc tttccatttt
     6721 cgctccacca gttcctcaaa acattcttcc aacacgatat aagctaattc cggcgcataa
     6781 tcatctcgtt ctgtttgaag catgagacat tcttgcaaac tacgtgcacc tactccagct
     6841 ggttctaatt gctgaatcag agtcaacgca tcgagcattt ggataggcgt agctccaatc
     6901 tcttttccgg cttcttctaa actaatcgtc aaaaatccgt tcaaatcgat gtattctaca
     6961 agataaagaa cgattttcct caaaaaagta tctcggtaat tcaaatggac ttgttcgatc
     7021 aatgaatcaa ataaagaccc ttttgtatca ggaatctgat ttaggtaatt tgtctcttct
     7081 ccttcataag aagatgaaga agcttttatg taatctggct gccattccgg ctcaacaact
     7141 tccaccaatg gattttccat tgcttgattc tcaacgaatg ccaacagctc ctcactgtta
     7201 tattgtaaaa cttggatgga ctgttgcaac tgttgcgtca tggctagttt ctgtgtctgc
     7261 tgctgttttt gtgaaaaatt ttgctcaaat ttcatttttt tgtccaatcc ccttgtaatt
     7321 ttttatctgt ttagttacaa ttatcccagt gcttgcgtcc ttagtgtagt ggatatcacg
     7381 taagattccg gttcttgaga cgggggttcg attccctcag gacgcgttct actgctattt
     7441 tagcataatt tacgaatagt tgcttcttta tttttaagtg aaaactgtcc gccaaagaaa
     7501 attggtggac agttttttgt tgtacctgta tagatattgt taagcctttg caagtaaggt
     7561 agttatgtgt tcaactactt attaaaatgt gttatttatt aaagatactt gtctcactta
     7621 ctgaactggc cgatgatcgt ttctgccata atagttctcg acagccgttg gatgccctaa
     7681 taaatcccca actgtcgtga tgcgataccc ttgctctttc agataatcga tcacttgagg
     7741 aacggcacga atcgtttcag gatagatatc atggaataaa ataattgccc catcgtatac
     7801 tgttgcttgg atcttctgca aaatcaaatc aggatttctt gtcttccaat cttctgaatc
     7861 aacagaccat tcgatgatcg gtcgctggat gatcgttgct cctacattcg tgacagaacc
     7921 atagggcgga cgaacaaaag tgggggtttt gccagtgact tttttgatct cagtgtctgc
     7981 tttcgtcact tcctccgtca tcgactgggc agaaagagtg gccagattct gatgatcgta
     8041 tgtgtgattg ccaatctcgt gccctgcttc agctgtctgc ttcacgattt caggatgctg
     8101 cgccgtattt tctcctaaga cgaaaaaagt ggcatgcacg tttttttctt ttaagattcg
     8161 cagaagctgc ggtgttgtgg tcgctcctgg tccatcatca aagctgattg cgacgctttt
     8221 gacagccgga tctcggctcg tttttgtact gtcttgtgtt gattgtgatg actgactttg
     8281 taccttttcc gctgattctt gaatggcttc tttgacgtta gctgtttcag ttgattggct
     8341 tttaatggtt gattgttctg attgtttgaa agattgtgac gaatgctccg aagagtgtgt
     8401 ttgtcctttt acttcggtga tcaaggaggc caccgaagta atttcgaaaa acaaaagcac
     8461 ggataaagca ataatactga taactaaata atttcttttc actatatctc ttctttctct
     8521 ttttctcttc ttattattta cttgtactta cttgaaaatt gaaactgcta gtcgcaacta
     8581 cttctcgtct aatgatccaa ataatcttcc gatgccaaaa tcgtttttgt cacggactta
     8641 ggaatgacaa attctggtgt acccatataa ccaggagcaa tttcgtattg agaaaaagtc
     8701 aaaactaatt gatgctcttt gttcaaataa aaacttcgtg taggcgtaac aagtgttggt
     8761 ttttctatcg tcccttcttg aatatcttgc tcagtccaat agtacttaga tggatcttcc
     8821 ttgacttgtt cggccatctg ccgtttgact tcttttgtca aaacagatag ataggtgtca
     8881 tctttgaata aaagtggaag tgagagctgg acactgtttt tcttgtcgat tgtgtcgtat
     8941 cttttttcaa catggctatc tgctgctgtt tgggtgatct gcctttcaat cacgagaaac
     9001 cggttatcgt ctacgatttt ctgataatct cccaaaactt ggtaatgatc cgtttcaaaa
     9061 tttccatatt ctttttcagc tttttcaaaa tctgcttgtc cttcgcgaaa gtatttttga
     9121 tttaatccat gaattgtttt attttctttc tcattttgtg tattgatctt cggtacctga
     9181 atatcgattt tttcagatcg atcagtaaag cgttcgccta agatcaattc aatgactgat
     9241 cctaagatgg gtaaatcttc tgcaaagctt cgaacttgac tattaaaaag aaggctactt
     9301 gttgtaatca ttacgatagc caaagatact ccgaaccctt ggagacgtct ctttctccgc
     9361 tttgctttta taaatgattg ctcttcgata ataaaacgct ggcggatctc ctctttaagc
     9421 cctttgggaa ttgcttgttc tttataagtt ttctttagtt tttttaaatt ttctttttgg
     9481 gaatacatgg cactcttctc tccttcattc taggatttga cgtaaagcat tcaatgagcg
     9541 gtacattttc gttttaactg tgttttcatt catatccaag atagtcgcaa tttctttcaa
     9601 acgaaatcct tcaaaaaagt agaggatcag aatctctttt tccggactgt ctattttatc
     9661 taaaatcata tggagctctg caacggaagt ttctaaaggt aactgttggg ggatatcttg
     9721 aatttcaaat aagtccacag gcgtcctttt ttgtctgcgc caactatcta atgccgtgcg
     9781 gatcaatatc cgataaaacc atgcactaaa atgctcgatc tctttttctt tttcaaaata
     9841 gttcagggct ttatgaaaac tatcttgcat cacatctaag gcctcttctt tatcatgtac
     9901 gtaattcatc gtgatacgga acattttttg ataatcagtt tcgatgagtc cattcagcag
     9961 gtgacggtat ttttcgatct gccgtttttt cttatatcca gtaaaaatca ttctccactc
    10021 ctttctgcct cacttaatat gacgtacaag gtgcgctaaa agtttcaatt tttttatttt
    10081 ttatgtattt acttgggatt tttatataca aaaaagaagg tgtgacaaaa atcgtgtcac
    10141 acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga aaataatgtt
    10201 tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc atcgtcttcg
    10261 tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc atctgtcggg
    10321 tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc atcttcatca
    10381 tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc ttctgggtca
    10441 tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac tttcttgcgt
    10501 ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac ttcttcatcg
    10561 atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa tgagataaag
    10621 ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat ttcactgtct
    10681 gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat gacatcgcca
    10741 cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt gtttgcacct
    10801 tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct tatcatacaa
    10861 aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc caaggtcaaa
    10921 taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg aaccttccct
    10981 gctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc aatctggatc
    11041 ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa tttcaaacgc
    11101 atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa tttgatcgtt
    11161 acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta cagcgtatcg
    11221 ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc cccttgctgt
    11281 tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc cattattcat
    11341 tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc aaccaacttg
    11401 tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag tataatattg
    11461 tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt cgactagatc
    11521 agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata ttaacggaat
    11581 attctaaaga acaccagcaa atctttttac atttctggca atatgactgc acagtcatct
    11641 taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca ctggaagatg
    11701 caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca gatgaaggaa
    11761 ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc atagatgacg
    11821 cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg atacaagcat
    11881 tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat ggcaaaaaat
    11941 ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg tacttaagtg
    12001 tcaacggtaa tcaggaaaaa cgtgggcaag tcatgaagcg attttatcaa tctgctttaa
    12061 aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg gcaacacttg
    12121 aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt gcaaaagctt
    12181 ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta aaaaaagaaa
    12241 tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat aacataaagg
    12301 agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa agtttttcgt
    12361 gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt gatcaattcg
    12421 aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt cacttttcac
    12481 ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc aagacgaatc
    12541 tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg ctgggatgat
    12601 caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca tggtccttac
    12661 tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt agagatcatc
    12721 acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg ttttcctgaa
    12781 aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca aatgatttct
    12841 agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt cactggaaca
    12901 gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta tgaaggcggg
    12961 atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag ccgatatcaa
    13021 atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat tttggagcat
    13081 ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga actccattct
    13141 caattacttc tgccatttct tgacggaagc tttactttag aagaatacct caaacttgat
    13201 gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc tattttaaat
    13261 gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt ttctggcaac
    13321 cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg atataatcct
    13381 gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta tcgcccagaa
    13441 caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt gatagaatta
    13501 tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga tgagcgattt
    13561 ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt atttgacgaa
    13621 acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat aaattaaaat
    13681 atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg tactttatta
    13741 aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc tgttgcaaaa
    13801 ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga acagttaaac
    13861 gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc actcgtacaa
    13921 caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga tttcctagag
    13981 atcgaagtaa tggcacgccg tgtcggcagt catctccatt cgatcgatga caaagcgatc
    14041 tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt agtcaagatg
    14101 ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt caaaatgatg
    14161 atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca aactttccgt
    14221 gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa taaagaagca
    14281 agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca agatgaaatc
    14341 atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg tctaggagta
    14401 gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac ttcatcaaac
    14461 gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat aaaaaaactg
    14521 agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc atctctcgac
    14581 tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc tcacccacca
    14641 aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag caacaaagaa
    14701 atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact catcagcttc
    14761 tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct cacggacaat
    14821 cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa aagcaccatt
    14881 tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg ctgcataacg
    14941 ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca tgaatagttt
    15001 ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat gaacaagcac
    15061 aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa ggagagatga
    15121 atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat caatcgaatt
    15181 ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag aaaacaaatt
    15241 tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga cttacaacta
    15301 tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag cagctgatga
    15361 tctagaaaaa ttacacgagc aacataaagc tgctggtctt gagattactg acttaaaagg
    15421 attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt ataaaatcga
    15481 agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa aaacaaacac
    15541 caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg tcttttttta
    15601 acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat tcatttgaaa
    15661 gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct attatcacac
    15721 gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga taagctgaca
    15781 attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt gctgatccac
    15841 atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca aagtcttttt
    15901 gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg atagctgtaa
    15961 aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat aaatcacgtg
    16021 ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac aatatcggca
    16081 cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt cttgtcacaa
    16141 atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg taatagttaa
    16201 ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct cgatctggcg
    16261 taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt tcagataaat
    16321 tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct agcacataaa
    16381 ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg taataatcta
    16441 agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac aacaaagatc
    16501 ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt tataaaatgt
    16561 ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct ctaaatctcc
    16621 ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt aatcatctac
    16681 atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag gagtaaatgt
    16741 gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa atgcctcttt
    16801 actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta ctgatattgc
    16861 atttataaac gttattttag gatactgttt tttagcttta gcgataaaag tatttcgtct
    16921 ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag aaatcgcagt
    16981 caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg catgcggatt
    17041 tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg tgactgcatt
    17101 tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa tctctcctaa
    17161 atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt ttgtgccaaa
    17221 atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc cttttccgtc
    17281 aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat aatcaaaaaa
    17341 acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta cttcctcaaa
    17401 attaataatt ttcatagaca attccctcct cacaaaagat taacttctaa aatcaataca
    17461 atcaatggta aaattgtatg gtatacaatt ttaaggagtg gagaaaatgc ctattaattc
    17521 ttatgacaat tacccgttaa cttggcgacc agacaaacaa aaattgaagc gtccttatta
    17581 tcgatcgttg attcaacaat tagaacaaga tattttaaag aataaattac agaaaaacac
    17641 tcagttaccc tcccaaagag agctagctga ttatttagat ttaaatttta ctaccgttgg
    17701 caaggcttat aaatatggaa ttgagaaagg tttgctgtat acgaatattg gaagtggcac
    17761 atttatttct cccaatgtct ttgagtccat caccatctct accaatgaag taaccgaaca
    17821 tatcattgat tttggtctgg ttagttcttt tgaagaatgt aataaattcg ttgtcccgtt
    17881 tattcaatca actagtaata acccaaatct tgttggtttg cttaattacc aagatcccaa
    17941 aggcacaaat tttcaattga ctactgcagc aaattggtta aaaactcaag gagtcattcc
    18001 gtccttagaa tctctatcta ttgtttccgg tgttcaaaat ggattggcta tcacactttc
    18061 tgcacttttc tctccaggtg atagaatagc aacagaccga tatacatacg ctaatttcat
    18121 tgaattggct cagttatatc atttagagat cgttccaatt gattttgacg agcaaggaat
    18181 gcgacctgat atattgattc aggaatgtag aaaaaagaag attcatggtg tctttcttat
    18241 gccttcttgt aataatccta tagggtttca aatctctcac aaaagacgag agcaattagc
    18301 cgaagtgatt gaaaaagaag gtctatgggt tattgaagat gatatccatt catttttaac
    18361 aacttactat caacatacga tcattcctcc tttccaaaat ttattaccta accaaacaat
    18421 ctacttagct ggaatgacaa aatttatttg ttctggattg cgagttgctt atcttgtagt
    18481 tccagtacat gtccaaagca agatagaaca agcaattttt aatataaatg tgaaaacttc
    18541 tggattagat gtggaagtca tcactcaaat cctccattca aatgaagcaa ataggatctt
    18601 agaaactaaa ttaagtctta caaaaagagc caatcagctt tttgattctc ttttcaaagt
    18661 agaaaaacca acgaatcgtg tcccttacta tcgaattctt ccagttgatc ctgagattga
    18721 ccctaagata attgagaatg actttcttgc taacggtatc cgactttatc attccagacg
    18781 ctttactaca cgtgcgcaac cagatgcatt cgttcgaatt tccctatcct ctaataacct
    18841 tgaaatttta gcttctggac taagtatcgt aaaagaaaaa ataaaaaaat atgaattata
    18901 acctattgat aaataaagga atcaaatctt attgattcct ttcaataaaa aagaggcact
    18961 acccgaggtt caacaacggg tagtgcctca aaaaataaac gaacattttg cacttaaagt
    19021 atccaagaag caagaaaaat tattttattt tcttatttta ctaaatcact aggtttctgt
    19081 cagaagatga aattatccaa tcatgacact ttcttctgga tatttgtatt ttgattgtgt
    19141 tctttgtgct tttgtcatca gagagaagac cacagtcaat acaccaactc gtccaatgta
    19201 cataagcgct ataatgatca atttccctat tgcagtcaag tctggcgtca atcccatcgt
    19261 aagacctact gtaccaaacg cagagaagac ctcaaaagca atatattcga tgccagaagt
    19321 tttaggtatc gtttcagtca ccgataaaat catgatggcg actacacaca acgtcaacgt
    19381 aacaaaaaat aaagtaagtg cccgcaacac tgcgccttgt cggatggtac ggccaaatgc
    19441 ttccgctctc gtacgtcctt tcaacatcga acgcatctgg atcagcaaca cacctaacgt
    19501 agttgttttc aatccgccgg ccgtcgaccc agatgttccg ccgatgtaca tcaaaaacat
    19561 cgttaaaatc agacctgcat ggctcatatg gaggtaatca actgaataat atccagccgt
    19621 tctaggtgtc acactcataa aaaaggtgtt tgccaatcgt tgaaacatat tcccttccac
    19681 tagcgttgcc ccattgcgct ctgtgaaaaa gaacacaacg aatccgccta ataaaagggt
    19741 acctgtaacg gttaatgcga tttttgaatg aatcgtcatc ttctttttct gatgatattc
    19801 cagtaaatca cgccatacga tgaagcctaa cccaccagaa atgatcaaag cagataccac
    19861 catgatcaaa taaacatttt gctggtgatt ggctaaactg tctcctaaca aatcaaaccc
    19921 tgcattacaa aaactagaga ttgcatggaa caaactgtac cagatacctt tcaaccagcc
    19981 aaattcaggc acaaatacaa aagataaagc aaccgtacca atcaattgga tcacaaaggc
    20041 caattttagg atgtaaagca tgagcttcat cacacctgat acttcttcta gattcagcgc
    20101 ttcttttaat ataatacgtg tgctgagact gactttcttc tttatcaatg caaagcatag
    20161 aatcggaacc atcataaaac caagaccgcc gatctcaatc aaaagcatga tcaaaaattg
    20221 accagcactg ttccaatgct ctgctgtatt caatgttgtc aatcccgtta cacagacagc
    20281 agaagttgca gtgaaaagcg catcgataaa atttgtccca tgcccactat tactaaaaaa
    20341 tggtagggta agtaggccgc cgccaactaa aatcaggaga aaaaatccta atgcgagaag
    20401 ctgaacagcc ggcaaacgtt tctttatttg tttgctttgt ttaatcatcg ttttcctctt
    20461 ttaaaaaaag ataccactta gtcagtggta cccatatttt ttactttgat caaccttgtt
    20521 acttctgcgc gctcgttttc ttccagcttc atacgaatat aatagatcgt ttcttctaat
    20581 tgagggatcg tcatatattc taatgcattg actcgccgac gtgtcttttc gatttcactc
    20641 gccatcagtt gacaagtttt ttcgatttct gtcagttcca aaagttttgg aagcagggta
    20701 gtaaaccgat cgattgaccg atccagttcg gcactggaat tcaaataccc gtattccaaa
    20761 ggtgtgtctg tcagctgttc atcatattca aaattcatca caggtacttt tacactcatg
    20821 atatttttgt ctacgataga caaatcaacg ttttccgccg gcaaaacaaa taattcttcg
    20881 atgaaaggtt ccgtgatcga agcatttgcc aatacaaaat cagacattgc ttctgtcata
    20941 gactcttcca tttcttgacg cagctggttg ttctttcgaa tcaacaggat gaattgacgc
    21001 atcagttcat cttgcttatc ttttaataat ttatgccctc ttgtggcagt tgtcaactgt
    21061 tttttcaatc gagtcaattc catgcgggtc ggattcacat tcaatcgtgc catcagaact
    21121 cacttccctt ctggcagata tttgtccagc atgtcgtctt tgattcgttt taattcggtt
    21181 cttggcaaca tcgatagcag ttcccaccct agatcaagtg tttcagtaat cgaacggttc
    21241 gtgtagaatc cttgattgac atattctttt tcaaaccgct cagcaaactt cgcatagatc
    21301 ttgtctacat cagataaagc agattcacct aaaactacgg ctaattcttt tgcctgtttt
    21361 ccttgtgcat aggctgcaaa aagctgattc atcgtagctg catggtcttc tcttgtcttt
    21421 ccgggtccag ttcccttatc ttttaaccgc gaaagagacg ggagtacatc aattggtggc
    21481 tgaatcccac tcttgtaaag ctctctagtc aagatgatct gtccttctgt aatataacca
    21541 gtcaagtcag ggattggatg agttttatcg tcttctggca ttgttagaat cggaatttga
    21601 gtaaccgatc cttttagtcc gcggattctt cctgctcgtt cgaataaagt agctaagttc
    21661 gtgtaaagat acccaggata tcctcttctt cctggaactt ctcgccgtgc tgcagaaatc
    21721 tctcgcaatg cctctgcata gttggtcatg tctgtcatga taaccaaaac atgcattccc
    21781 ttttcataag caagatattc tgcagcagtc aaagccatac gaggagtagc gatacgttcg
    21841 attgcagggt cgtttgctaa attcataaac atgacagaac ggtcgattgc gcccgtttga
    21901 cgaaaatctt ccatgaaaaa ttcagcttct tcaaaagtga ttccaatagc agcaaagact
    21961 acggcgaaat catctgatga atcgagtacc gttgcttgtc gcgcgatctg agcagctaat
    22021 tctttatgag gaagaccaga gccagaaaat actggtaatt tttgcccacg aactaatgta
    22081 ttcaaatgat cgattgcaga gatacctgtt tggataaact catctggata atcccgagct
    22141 actggattga ttacttctcc gttaatatcc aaatgctttt caggcaggat ctctggtccg
    22201 ttatctttcg gtcgtcccaa accatcgaat actcgaccga tcatgtcttc cgatacacct
    22261 agttctaaag gatggcctaa aaatcgtaca gaagagtcac gtaaattgat accacttgtt
    22321 ccttcgaata tttggaccat tgctttatct tcttggactt ccaatacttg tccacgacga
    22381 atctcgccat tttgcatacg gacttcgatt aattcttcat atttgacacc agcaactttt
    22441 tcaaccgcca tcaatggacc gacgacttca ccgatcgtac gatattcttt aatcattgct
    22501 catgcctcct tctgaaacga tcgtttgcat cgtttgcttg atttcaccaa taatttgatc
    22561 tagtttttct aattctgttt ccgggatata tttgcttcgt ccgatccttt cacgaatcga
    22621 tacggtaccg tccatgatct cacttaaata agcacctaac gacaacgcac ggcgtccttc
    22681 ttcaccaaag gtcaagatca tcttcaacat tttgaattgt ttctctcttg aagtgaatgt
    22741 atcgacatca tcaaaagcat tttgctgcaa ataatcttca cgaatggatt ttgccacttc
    22801 taatgtcaga cggtcattat ctgaaaggga atcaatccct accaaacgca cgatttcatt
    22861 cagctgctgt tcttcctgca aaatacgcat gccctcggtg accatggatg cccagtcagc
    22921 ttgaaggata tgatccatat acttccctac ttctgttgaa taaagagaat agctttgtat
    22981 ccaatcgatc gaaggaaaat gtcttttttg ggctaaactg gaatcaagac cccagaatac
    23041 ttttacgacc cgcaatgtat tttgtgtgac tggttccgaa atatcccctc cagacgggga
    23101 tacagcactg attgcggtta tacttccttc gcgaccttct gatcctaatg caatgactct
    23161 tccagatcgt tcatagtatt ccgcaagccg tgaaccaaga taagctggat aaccttcatc
    23221 accaggcatt tcttccaaac gtccgctcat ctcacgaagt gcttctgccc atcgtgaagt
    23281 ggagtctgcc atgatagcaa cgtcataacc catgtcacgg aaatattccg caattgtgat
    23341 tcccgtatag atagaagctt ctcgagctgc tactggcatg tttgatgtat tagcaatcaa
    23401 taccgtacgt tccatcaacg attctcctgt attagggtca atcaattcag ggaattcatt
    23461 taaaacatcc gtcatctcat ttccacgctc gccacatccc acatagacaa ccatgtccac
    23521 atcggcccat tttgcaattt gatgctgaac gactgttttc cctgcaccaa aaggaccagg
    23581 tacagctgct gctcctcctt ttgtcactgg gaagaatgta tcaatcactc gctgtccagt
    23641 gatcatgggt gcatccgggt ttaatttttc tttgattggc cgactacgtc ttactggcca
    23701 tttttgaagc atcgtcaaat ttttgtcacc attttcggtt tcaatgacac atactgtttc
    23761 atcaatagtg aatgaacctt tttcgattgt tttgatcgtt ccttttatcc catttggcac
    23821 catgatttta tgttttataa cttttgtttc ttctacgatc ccgataacat ctccagcact
    23881 caccgtttct ccggcttcta ctgttggttc gaaccaccat tttttctcat ggtccaatgc
    23941 gggcaattga actccgcgcc ctaagaaatt actttgtgtg atttccataa aggtatctaa
    24001 tggccgttgg attccgtcaa acatttgtga aatgatccct ggtcctaatt caacagataa
    24061 agcttcacca gtagagcgaa ccggttcgcc tggaccaatt ccagaagtct cttcatatac
    24121 ttgaatagac gccacatcgc cacgcatctc gataatttct ccgataactc ctagatctcc
    24181 cactagacac atgtcttgga tactggcatc agacatgttc tcagccatta caagaggtcc
    24241 ggagactttt atgatttttc caatttgcaa aatttctatc tccttttcat actataagat
    24301 attttgacct actgcttttt ctacgttttt ttgaatttct tccagcccta tccctaatga
    24361 tccttgatga ttgggaatca atacgatagc aggtatcatt tgttccttgt aacgttcgat
    24421 cgtttctggg ctcaatgctg cacattgctc tgtgacataa atcacgccat actcattctt
    24481 tgccatttct tcaattgcct gacgaacggt ttgcttggtt gtaccataac gaacatcaaa
    24541 gccaaacaat ttgaatggca aaacagaatc tttgtcgcca actacaccga tcttatgcgt
    24601 catagacttg tctcacccgc tttctgattg tttctgattc gaaatggctt cttttgccga
    24661 taaccagtaa acgaaggttt ttgctttcta cctcttttgc gttcaaaaag gctagtagtg
    24721 gcagtggtcc gaaagcctgg gtttgtgctt cttgatacaa tgaacttaga taatcatctt
    24781 tcaactgttc aagctttacg agatcaatat ttccctcacg gatgacaggg cgcagaaaat
    24841 cagcataatc tgtctctaat agaaaattcg tgtaatcttc catatcttta tctacaaagt
    24901 tcaaaaatac ttctttttcg atacttcccg agctggaaag taccgtcgtc atgaacccat
    24961 atgttctttt ctgcaatatt cctcgagcca tcgttgtgat attggtcaag tcaataaaag
    25021 caatgacttc tgtcagcaat tcttgatatc ctagcttatc ggcaagccgt cgctgctctg
    25081 tcaaaaactg tcgatcataa atcacgtcaa ttccctgaag aatggtagac tcttcaaaat
    25141 aatcacgtac ttctcgtata ctgtccataa tgctttcagg aagttcattt gaaacttgcg
    25201 tatgaatggc atccttcaag gtttctaatg aataaaatcc atcgtcgata tacaaatggt
    25261 ccagattttg atttgtcatc tcagcttttg tcaaaacctt cagattatgg aaagtataac
    25321 gcatcgtata gatccaaacg atttcaggct ccggtgctga ttcctttaac cagcgaaata
    25381 gacggctctg ctcctttaaa agattctgtt cgaagtcata ttcatatccg tcataaatat
    25441 atggactgta aatcgtggat ttcaatattt caccaagtgc ttcgacagaa tttgtttgaa
    25501 tcatccgttc aaatgtctca ttggacaaaa gttcaagctc tcttccgcga atcaagggat
    25561 ttagttcgtg gtattccata cactgatccc tccttattcg aataattgat tggcaatctc
    25621 aaaactcatc gttccttgga tatcttgtac aaggctgctg aaaagaaaat tatactgaac
    25681 tccttgatcg tttatcagaa aacccgcttg atcagcaaca gtttcatcac ttaaaaccat
    25741 cataaaggga agttcattgt tccattcatc caacaacgtc tgagaaagat aggctgctga
    25801 tttttctcca gcaatgaatg ctacttttcc tgtcaaaggt aatgaataca atgcattttt
    25861 gataaactgg atttgttcag attcatccca attttccatt tcagttaccg catctgcaaa
    25921 caatcgtctt aaaaattcct gcttcgcatt caaattttct tgtcgtactt ctacttgctg
    25981 gcgattcctt agctgacggt attttttttc gagtaattcg atttgttttt ctttttgttt
    26041 ttgatgttct gtttcaactt gcgtacgttt ttgttcaaag ttttgatcga tcttcattcg
    26101 ctcttcttgt tctaacgccg ctctttcctg ctctgctgct tcgttcatct gagagatgat
    26161 tttttcaatg gcgttcatct gcttttcctc ctttatgctt aggcattcaa cactaatagg
    26221 aaggaaataa cgaaacctaa aatcgcgtat gtttcaacca tcgcagcaaa aatgattcct
    26281 tttgtagcat gttcaggttt tttcgctaaa atttgaatac ctgccgctgc gacttttcct
    26341 tgggcaatac ctgaaaacaa gcctgtaaaa gcaatcggca gagaagcacc tagaaagttc
    26401 agtccttgta caactgacat gtcatttcct aagttgataa aaatcaaaaa ggcaataacg
    26461 aatccataca aaccttgcgt gcctggaagc aattgcagaa tcagagcctg cccgaatttt
    26521 tcgggttgac ttgttgtcaa tgctgctgct gcctcacctg tcattccaac acctttagct
    26581 gaaccgatcc ctgaaaaaat cgtcgctgtt gccatcgcta ataccgcaaa taccattcca
    26641 ccattttgac taattaagta atccatcatt atataaaaat cctccaatta tttattgttt
    26701 tttttcttgt gattgatgtt gacgtatttt tccgccgttt tcagcggatc aaaggcacgt
    26761 ccgcctcctg tataaaactt cccaaaaaat tcgacatact gcaatcgtgc gccatggaca
    26821 taggcactta gcagtgtcaa aaacatattc agtgcgtgta aagcgatgat caaaaggatc
    26881 cctacactga atcgagcagc tggcggcatg aaagcaacta gcatattgaa tgccgctcca
    26941 atacttccgc ctgaaatacc caatgccatc aaacgtgtat aactgactaa atcaccgata
    27001 tagccggtca gtccgtaaag attatacgca cctttggcag cacctttgac tttagacggc
    27061 gtttggataa tcggaacgat caagatacat aaggcagcaa tgatcgatag cccagcgcca
    27121 aggtaaacaa atgctgtatt ttttatcaac atggcaccaa ccaacgcaat aacgatccca
    27181 attaggatcc cttgccaggc aaatccatca ctgacagcac tcaagtaatc ttttctcctg
    27241 atattttcct ttgctgccac aaataatcca accaaaatct gtatcagacc gaaaatcacg
    27301 gataaaatca aaatcgtatt gacgtcatcc gtcgtagata ggattgggaa aggcaagcgg
    27361 atgccaaaca gttcttttgg taatgactgt ccgaaaaatg aactgtagat caatccccag
    27421 atgatcgatg gtatcgagag aatctcaaag aattttacga aacgtttcat cccgcgtggt
    27481 agaacaagta gtttttgagc tagtattgtc ccaatgagca tcagcaaacc ataaccaaca
    27541 tctgcgacca tcatcccgaa gaagacaaga taaaatggtg tcatccaagg tgttggatct
    27601 acttcttcat attttggcag actgtacatt tcagtcagca attcaaaagg ttcgacaagc
    27661 ggatggtttt tcaatttagt agggacttcc tgctgaatct cttctgagtt tggtgtttcg
    27721 aaactgacgt atatatcttc ttctgatacg gtttcgttta acacatgcag caaattctgt
    27781 ttttcttctg catctaccca accttgcaaa acgatcagat aagtcgcatg gataaaccgt
    27841 tcttttattt tttctcgttc tgcaacggca agtgtgactt cttctaccca ttcaaattct
    27901 tggatatagc cactgcactg acctaacgct gtcgttatct tcttttgctg ctcgtgaaca
    27961 atggataatt gctgcttggt ctcttgcagt ttttctttag gattttgttc ataaggatac
    28021 gtttctttat tgaacccgta tttcccagca atttcttcta taacagcaac tgattttttt
    28081 aaagcaacat acgaaagata aacttgtttt ggcgtataat ctatttcttc catataaacg
    28141 tcatcaagct tactcagttc ttccttgaaa ctttcaaaat ttgctgcact cacagtcccc
    28201 atctcaaaaa tggtcgtctt acttgcatac atacttggag caatatccag ctgttgccaa
    28261 tgaagaagcc attcttcctc ttcttctaaa cgatcacgtt cttcagttag ttgttcccac
    28321 ttttctttta aatcaaggac ttctttcaat ttatttatga aattttcttc ggaataattg
    28381 gcttccaaat cttgtaagga aagttcttga cgtttcaaat ggagtttctt ttgacggcta
    28441 tcgccgtggt gttgtatgaa ttggatagct tcttggatgt ctttcaaccg attacctaaa
    28501 gcgcttaatt ttgcttcttt atcaataatc gtggtatctg gaaaatactt ctccacccac
    28561 tggttgtttg ttctattttg atacagatcc cggatttcaa cgttttggat tccttgtact
    28621 gcttgtaaaa tcctctcctg atttctttta tctgaaatga gggtcacttt ttccatctta
    28681 gtgacggcca taagtttctt tcaccctttc aatgatcgca ccgatcgctt gactctgatg
    28741 cttttgatat tgatctttca aagttgcttg caccttgtct gcttctgctt tcaatttcat
    28801 tttttctttt tctaattctt gtatttcaaa ttgttctttt tcttccacca actgttttag
    28861 ttgttgtcga tagtttgttt ggatcgtttc caattcctgc tctttttctg ttgcatagat
    28921 cttcagctgc tgccgcagtg tttgagcacg cttttcattt tcttcttctg cttttcggat
    28981 ttgttccaaa gcttctaaca cttgcttcac ttcctttcga tttaaaaaag aggtacatgc
    29041 tttctttttt cattgaaagt atgtacctct aaagataact tagtataggc aggccccttc
    29101 aataaaatgt ccttctcacc ccttgaagaa ctagatcctt gaagcttacg cccaaccttt
    29161 ttatttctgc ttacttttta ttataacaaa ttaagcaata ttttcaacta tcgtcatgaa
    29221 tttcacacga aatttctctt tacagatagt aacacagcaa gaacagataa gtataggggc
    29281 aagtcttaca tagtttaatt ttttttaaat ccttgttaca ataagtctac tttaggggga
    29341 atacaaatga aaccaatcat cggcattgcc ggaaatcaac tgattcgagc aacagatact
    29401 ttccaaggaa atcaagtgag ctatacaccg caaggattcg tggatgcagt tttagatgct
    29461 caaggattgc ctcttattct gccagtcatg tctccagatt cagctcctca actgattgga
    29521 caaatcgaca aacttattct tgcaggtggt caagatgtca gtccacaact ttacatggaa
    29581 gatcctcatc caaagctaac ggaaacaaat attcaacgag accaatttga acaggcactg
    29641 atcttggaag cattgaaaca aagaaaacca atttttgcag tctgccgggg actccaacta
    29701 ttgaatgtcg ttttggaagg tacactttat caagatcttt cattatatcc aaagtggtca
    29761 gtaaaacatg aacaacagcc cactgccccg caatttacga ctcacgaagt caagattgtt
    29821 tcagacagtt tgttaagcaa ccttctcccc gatccatatt ttgtgaattc ttaccatcat
    29881 caggcagtca aagatctttc acctttatta aaagccatcg ctttttctaa cgatggactc
    29941 gttgaagcgg ttcaaagcaa agatgatatg cataaaatcc tcggtgttca atggcatccg
    30001 gaacttaccc accgtgtaca tccatctgaa cagcgcttat tcgatttctt tgtgaatgaa
    30061 ttttaataga ctttctgagg gggttgtgaa aaagctgttt agtttcgagt attaagaaaa
    30121 attttgtgaa aatggctttt cacatttttg cataaatttg gcttaatacc gagaaactgg
    30181 cttttgaaca ccgtttatac caagaggtcg tgagcctgcc gttcaactct gagtcacaag
    30241 gagcaatttc aaaaaacagc ttttcatgtt tgttgaatag tgtgacttgt gaccgaggag
    30301 ttggcagaag aacaccgttt atccggaaaa agagactgcg acacgacttt tgtctcagtc
    30361 tccagtctat ggaacaatgt tcactggaat tcttctcaat cgtcctttcg atagtccaac
    30421 agccttttga gctgggtaat ctgctgctgc agttccatac cttttgttgt atcacgattg
    30481 agtttgcgtg gtagttgctg ttcgattact tttttcacat ttgtttggtc ggattttttt
    30541 gcaaattgtt gttcaatagg caaaaacgga taatgtgtca cgatttggaa accataagaa
    30601 ttgcataata gtgaataacc ggcaattcct gtcgtttttt gataagcttt ggataaacct
    30661 ccgtcaatga caaatacttt tccatttgct ttgatcggtg actccccttt ttttactttt
    30721 actggcgtgt gcccattaat gattgctgac ccttcttctt ttgtgtcgaa ttctgctaaa
    30781 attttttcac tgaaccactc ttcatctcgc aagtgataat aaggattgct tttctcgacg
    30841 tgcgtatgag gctctgtgag aaaataacgt tcaaaagtcg ccattttact cttgccgaat
    30901 aaaggcgaaa atggcccact ccacgcatac caaatcaaat cggttgctaa atcttctttt
    30961 gcagtcagat cttttgcgct ttcagttatc tgtttttcaa aaaagcccag cagttctttt
    31021 ccgcgataag attggtctaa ttcaaatgct aagagctgtc ccttctcgtc tacaggtata
    31081 cagccatgaa acagcacatg ctgattgttc gtgagataca ttcccccttt ttcgatcaaa
    31141 aacgtcatat gtttttgcat tctcaaagag tgctggaatg aatacatcaa actatcaatc
    31201 actttttgtt cctcttccaa gaattttttt ggatcatctg gctggatcgt ttgaaaacaa
    31261 gttcctttca actcatagag ctgaccttct aaatcaatcg ttgatgcttc atagtcgatt
    31321 ttttccagca atttacgatc gtccatttta aattcaggtc gacgatccaa taactgatgt
    31381 tctgatttga attgaaggat agaaagtgcc tgatggattt tttctaattc ttcttgctct
    31441 ctttcggata gatttttact gtttttaggc gcaaatactg gattcttctg ataagtttga
    31501 tgagcaaaag aaaacaatgg acgtaaattc aacccatatt ctttctctaa ttcaaataaa
    31561 taattatagc gtgctgcaat ccgtaaaagt gtcagtaaac atgcctcaga gccgcaatac
    31621 gcaccaatcc acaaaatatc atgatttccc cattggatat ctacagaaga aaaatccatg
    31681 attcgatcca tcactttggc tgcttgagtt cctcggtcaa aaatatctcc aacgatatgc
    31741 aaatgatcga tgacagattt ttggatcgtc tgtgcaagtt tcgttaaaaa tttttcttct
    31801 tccttcatca aaacaagttg ttcaatgatt ttttttacat agtctttttt ttcattgata
    31861 cggtcatcga tatacaagag ttcctcaatg atatagctat attctttcgg caatcctttt
    31921 cgaacttttg accgggtata tttgactgtc gtaaaccgta aaagatcgat caaatgatgg
    31981 atcagctgcg atttatcttg ttctttgtac caatcagtcg ttaatgcata ttctggataa
    32041 gccgtaaaaa tagtgaattg gctgatatcc gtttccgtca tttgttcaga aaaaaggtct
    32101 ttgatctttt ctttaagatt tcctgaaccg atacgcagaa tatgatcaaa agcaggaaat
    32161 tcgccatgta tatcactcaa aaataactct gttccttttg gaagctgcaa aattgcttcg
    32221 agattgatga tttccgtgat gacatcctcc gttgagggga atttttctga aagtaaatct
    32281 aaataacggt ccattaaaac gcctccaaaa attggtatac tcaatattat aaagttgtta
    32341 tacactattt tcaatgaata tatatccaat atagtaaaat aaataaaata ggtctataca
    32401 aaaagaagct gggaacaata gatgtccagc ttccttctgt attttaggat tgttttatgc
    32461 tttttttcgt tcattcctca taattgaaat cgattgtttc aatgaactcc ataatccgcc
    32521 ttcgctataa acgagtacat tcgatttgta catgtttgca gacaagatca tcaataagaa
    32581 tgttacggct acaagtacca acaaggaaat caatgctcca cccataccga ctgtctcatt
    32641 tgctaagcga atcggcataa tatatgaaga taagaatgga atatacgaag tgatccgaat
    32701 aacgatattg tttggatctg aagctcctag tatcaatcca agcatgtatc cgcctagaga
    32761 tagataagtc actggcaaaa ttgcttttgc tgtatcttct gctttgttga ccaaagagcc
    32821 acataaagct gctaaaacag aaaaaatcaa aatgccaaag atcatgaata gaagagaata
    32881 ccacaagaat ggtccaatca agttatccaa tgagataccg tctaatatat ttttgaccat
    32941 ttccatatct ttgaattgac tgtatcctat accaaaaata ataccgtata atgccatttg
    33001 cgttaatgca acaagtaaaa cacctgttaa ttttccgtaa aaatgctttt gtgcagtcgt
    33061 actggacagg atcacttcca tgatccgcgt gcctttttca gaagcgatct cttgtgcaat
    33121 gatctgcgca tacgtcaaaa taataatgaa caagacaatc gttgctacat aacttacgac
    33181 gtattggact gtcgagttat cttctccgac tgtcattttt ccttttgaat caaaattgac
    33241 tttttgtcga gtcaaacttg cttgttggct caagctagct acttcttctg gtgaaatgcc
    33301 taattgactc gctcgaagca tcgattgcaa gccagtcagt tgttgttgta tcagtagttg
    33361 tgtactttgt cctaatgaat tttcgctata aagcatccca tttatcttat cattctcagt
    33421 tgagactact aaatacgcat cgattttttc ttccgaaagc tctttttgtg ctttttcttc
    33481 agaatcaact gctttgaact caaaatcatc tgttttgact tggctcattt gctgactgat
    33541 ttgctcatta tcagccaata cgccgatttt attgacatca ctattttgtt gagcaaagtt
    33601 tcctgctaca taaatgatac ccagcatgat aaaagggact aatatcatga tcaagaaaga
    33661 aatcgactta acatttttta aatagacatc tttggtaata atccaaaact tacgcatcga
    33721 tttcacctgc tttcaatttg aaaatctctt ccaaagttgg cggttgctgg ttaaacatag
    33781 gaatgtagcc gaactgagtc gcacgagtaa agatttcttt cccagcttca ggatcttcta
    33841 aggtaatctc taacgtattg tcttcatgaa ggatcacttt tttcacgcct tcgatttctt
    33901 ctacttcctg ttggcttaaa ccagattcca agaataactt cgtacggcca aatgattcac
    33961 gaatctcatg tacttttcca ttcaaaacca tttttccgtt tcgcaacatg atcaaatgat
    34021 cgcagatttt ttctacatta tccatattgt ggctagaaaa aatgacacat gaaccatgtt
    34081 ttttcaattc caatattcca tcttttagca attcagcatt taccggatct aacccgctga
    34141 atggctcatc caaaatgatc aatttaggtt catggattaa cgtggcgatc agctggactt
    34201 tttgctgatt tcctttagac aaagatttta ctttatccgt ctttttccct tttacctgaa
    34261 atttttccat ccaaaagtcg atttttggtt caatctcttt tcttgatttc cctctaagtt
    34321 ctgcaaaata aagcagttgg tcttgaatcg ttactttcgg ataaagtccc cgttcctccg
    34381 gaagataacc gatgacatca taatctttct ccccaagatg atggccattc cataacactt
    34441 ctccttggtc ttgtgtcaaa aaatccaaga tcaaacgaaa agtcgttgtc ttcccagccc
    34501 cgttttggcc aattagcccc atgatttttc catcggggat ttggaaagag acatgatcga
    34561 cagctgtgta gttcccaaaa gttttaacaa gatccttgac ttctaacatt tcttcactcc
    34621 ttcttcaaga aatcacatat atccttagac accagacgta agcgatttat tatttaccaa
    34681 caaaacgtaa acgctctttt tgttgtacat aaaatgatag gacactttac tttctttgta
    34741 aagtgccctt tcctcattcc tgatgtaatt tatcaaatac gttttgtgcg acatttttcg
    34801 gcagtccgat ttcttgtaat tcttctgtcg tggctgctgt gatatttttc aacgatttga
    34861 actctttaag cagcattttt tttcgttttg gtcctaaccc ttcaatttca tctaatcggg
    34921 aagcaaaact gtttttactg cgtagctgcc gatggaaagt gattgcgaat cgatggactt
    34981 catcttggat tcgctggagt aagaaaaact ctggtgaatt tcgctcaagc ggtactacag
    35041 ccaaatctgt accgaatagc aactcatttg ttttatgttt atcgtttttt accatgccag
    35101 caataggaat atcgattcct aattgatttt ctaagacatc ttttgccaca tctacttgtc
    35161 cttttcctcc atcgatcaag atcaaatcag gtaaaggcaa cccttcgcga atcactcgcg
    35221 aatatctgcg atagatgact tctctcattg atgcataatc atccggtccg ttgactgttt
    35281 ttattttata tttccgatat tcttttcttg ccggttttcc atcaatgaat acgaccatcg
    35341 cagccacagg attcgtcccc atgatattag agttatcaaa agactcgata cgtattgggg
    35401 tcgggatatt catcgcgttt cctaaacgtt caactgctcc gatcgtccgt tcctgttttc
    35461 tggcaatcag atcaaaccgt tcattcaaag caactgaggc attcttactc gccaattcaa
    35521 ctaatttttt cttttctcct cgttttggtt gcagcacttt cgtagctaac aaagcttcta
    35581 cactgggctt gtcgatcgta tcaggaatca agacttcttt aggaatgaaa tgctcatttt
    35641 cctgataaaa ctgaccgagg taagtcaaga aatcttcttc tgcttcgtta tagaaaggaa
    35701 acattgaaac atctcgttca attagctttc cttgtcttac gaaaaacact tggacacaca
    35761 tccatccttt atcaatcgca taaccaaata catctcgatc cagtaaatca gtatttgtca
    35821 ttttttggcg agtcatgatt gtctcaatgg cacggatctg gtctcggtat tctgcagctt
    35881 tttcaaactc catgttttca gcagcttttt ccattttctg atggatctct gactcgattt
    35941 tttcgtgtcc tccgtttaaa aagtttttca cttcatcaac gattttcgta tagactttcg
    36001 gatctacgtc gaaataataa ggacacaggc attgtcccaa atgatagtaa agacaaggct
    36061 ccttcgtctg actaggtttg cattttctca gaggaaataa tcgatcgagg atcttctttg
    36121 tctcattcgc cgcaccgaca tcgggatatg gtccaaaata cagtgctttg tcttttagta
    36181 ccttacgagt aatcaccaaa cggggatatt tttcattcgt tatcttaagg aaaggatagg
    36241 ttttatcatc ctttaacata atgttgtatt taggatcatt tttctttatc agattgatct
    36301 ctaagagcaa tgcttcgata ttcgattctg taacgatgta ttcaaagtct tcgatttcac
    36361 taacaagccg ttccgttttc gtattatggc taccagtaaa atatgagcgg acacgatttt
    36421 ttaatatttt ggctttgccg acataaatga tcgttccatt tttatctttc atcaagtaac
    36481 agccgggctg atcaggaagt aaagctaatt tgtttttgat tcgttcgttc atctggtact
    36541 ccccttcata ttcaattaaa gttattatag catgtctgtc aaaaaaacaa tccagcctga
    36601 caacgtaatt atactcaaaa aatattaatt tgaataacct taactcaacc gcaaaaaaac
    36661 cgaggacgga actggattca gtcccaccct cagcttattt ttgctgtaag tttttataaa
    36721 tattgatcga tcatttgacg tagttgttct tttgaatgaa caccaacagc tttttctact
    36781 acttgaccat cttttttcaa cattaatgtt ggaatgctca tgataccgaa ttgttgagga
    36841 gtttctggat tttcatccac atccatttta gcaattctga attcttcttc atcatattct
    36901 tgttccaact gatccaaaat cggtgcttgc atacgacatg gaccacacca agttgcccaa
    36961 aaatcaatca atacaagtcc tttatctgtt tcttcattaa atgtcttatc tgtaattact
    37021 tgtgacatat caaagacctc catttttaaa atctatgact agtatagcat ttctatactt
    37081 ggatagcgaa ccatctgctt acctttagta agctttactt aaatttaaca attgtcgcac
    37141 catttccgcc ttggtttgcc ggtgcaaact caaagctttt gacgctccga tggtttttaa
    37201 gataatcagt aattcccttg cgaagtgcgc ctgttccttt tccgtggaca atagtcactt
    37261 gaggataacc agctagaatg gcagaatcca aatattgatc tacctcactt agcgcttctt
    37321 cgtatcgttt tcctcgaagg tccaattgat tcggcacgtg actactagat tcagatcgta
    37381 cagcatggat aactcgttgt gtcggctctt tttgaggagc aacaggcgtc agctcgtctt
    37441 cggacacatt catttttaat ataccgattt ctacttgcca ctgattcccg tttttacgta
    37501 aaagcgtccc tctttggccg taagtcgtaa ccaacacttc gtctcccggt ttcaacgttt
    37561 tttgttcttt cgccttcttc aatactttat ttttcttcaa agtttcttct tgatgtagat
    37621 ttgctaattg ggtctttgca tcgatcaact gatgctcttt cacatttcct tgcccaatct
    37681 gctgttgcat tttacgaata tcagcaatga ttttttctgc tttttcttcc gcctctgaca
    37741 cgacttcatt tgcttttttc tttgcttttt ccatttcttt ttcacgttct tcaaagaaat
    37801 agctgtaagc ttctttcaaa tcatcgtgaa gttcttgtgc ctcagaaaca aaatgacgca
    37861 tttccagata ttcggtctct gccattttcc ggcgattctc taaatctgtg atcatttcgt
    37921 ttaaatcttg gctctcatca ttcatcaact gcttcgcttc atcaataact tctgtatcaa
    37981 gacctaatcg gctagagatt tcaaacgcat tgcttcgtcc aggaacgcca atcagcaaac
    38041 gatacgttgg acttaatgta tccacatcaa actccatgct ggcattaatc gtatttgccc
    38101 gattatatcc ataaactttc aattcaggat aatgcgttgt tgccatcaca taagcagact
    38161 ttttaccaag atcatctaag attgcgattg ctagagcagc accttcttgc ggatctgttc
    38221 ctgcaccgag ctcgtcaaat aataccagac ttttttcatt gacttttgat aaaatatcta
    38281 cagtgttcgt catatgagaa gaaaaggtcg atagactttg ctcaatcgac tgttcgtctc
    38341 ctatatccgc aaatacttcc tcaaaaatac cgatttgact ttcttcacct gcaggaatcg
    38401 gtaatcctgc ttgccccatc agttgtaaca gacctaaagt cttcaacgtg atcgttttcc
    38461 cacctgtgtt aggtcctgta atgacgatgg cttgatagtc ttttccgatt gtgatgtcat
    38521 tagaaactac tttttcctga tcgatcagtg gatgtcttgc ttgtttcaag acgacatgat
    38581 tgtcttcact gattcccggc acgatggctt ttacttcttt accaaatcgt gcttttgcat
    38641 tcatcagatc cattttacca atcacataag tattgtggat gatttcttga cgatggggaa
    38701 caagttcagc agaaagttca cttaaaatac gttgaatctc atttcgttca gcgatttggt
    38761 attgacgcaa acgattattc aaatccacca cttgttttgg ttcaataaat aaagtctgac
    38821 ctgaagcact ttgatcgtgg actacaccgc caaatacacc gcggtattct tgtttgactg
    38881 ggataacgta gcggtcattt cgcatggtga tgatcgcatc acttaaatac tttgcatttt
    38941 tcccacggac gatcccatct aactgttcac gaacagcttg ttcacttcgc cgaatgttct
    39001 gtcttatgct ctttaactca ggagaagcgt catcagtcac gcgtccgtct tcatcgattg
    39061 cttcctttaa acggcggctc aaaacaggga tcgtgactaa ctgatctgcc cacatataca
    39121 aacgtgcaaa ttcgatttca ctgtcagaaa gatcatcaat gaaacgattc aattcgcttg
    39181 tcgtagacaa tactctggaa acttgtgcaa gctccactcc attcaagtct gctccgatct
    39241 caatccgttt catatgggga cggatatttt caattttagg tatagggatc ccgcctcgca
    39301 gacgttgcac tttcaatcca tcctcagttt cttgaagcca gtttgtgatc gtttgtttct
    39361 cactcactgg taccaattct gccagttctt cttttccttg tgcagtcact acaaattgtc
    39421 tgaccatctg tttgactttt tcaaattcca atgtttcaag gattcggtta ttcattttat
    39481 caccttatct ttcactatga aaaaagagct gtgccggaca tacgctacgc agccttctta
    39541 aataaactac tctgtacaaa aaacgaaata ttttccagca ctattttcct taattcaaaa
    39601 tattcgttac ccaaagctca tacatcttgt tggtaagaaa tggcgttctc tcaacgataa
    39661 aacgagcgaa actatgctcg ccgaattgat tttggaccac atctaatgga acaaaggcaa
    39721 gtaactgcaa aatcaaaaat atagtaatgt aagccatgat tagtgacagg atgcctcctg
    39781 caatccagtc tacctgtctc aagatcggca catacgtcaa tccatggaca aaaaccccca
    39841 caaatcgggt aagcagccag ccaattagca aaataatcag aaaagcagtt cctgcataaa
    39901 aagcttcatc taggtgaaag gcaaatacct gatcaaaaaa tgctaacttt gatgttgggg
    39961 tgacagctgg gtatggaatg tataattcca aatgacttgc taatggtttg taaaaatgct
    40021 gggcagcaat aaatgacaat acatatccaa tcgcgaatat gatttgcaaa gcaaacccgc
    40081 gacgtgctcc agaaaaaaac gcaattagca ataaaaataa aatcagcaaa ctcagcatgc
    40141 tacgttcctt tctcttctaa cttttttaaa tttttcttat tttgataaaa ggatcttaat
    40201 tagaagattt ttgtttaatt tgtccttttc gtttctcgtt taaaatctgt tgtgcttcca
    40261 catgattttt aatggtatga tctgcttgac cattttcttt caatacttcc cgagcttcgt
    40321 tctcgattgc ttcaatgcgt ttcacacgat tttcaagctc tgtaaatttg atcatttttt
    40381 tctttaattc tgctgtttct tcttccagct ccaatattct ttcttgtttc ttcaattgat
    40441 ccgaaagcgc attgacagcc atcaagattg ctgcttgttc attatccatt tgtggtgata
    40501 attgtttcaa ttctgctaac tgttcattga tcagcttcgt tacgatatcc atatgatgct
    40561 tggtttcccg tccgataata gtatatgttt ggttggcaat caccgctttg tagcgtgttt
    40621 tttcgtgcgc catacgaatc atttcctcct atttgatgaa agttttattt agtgtaccat
    40681 gaagttggtt ctttcaccgt tctccacaaa tatttttcac atacttaact attatatgat
    40741 aaactttaaa aaaatgctat gtctcgcaat ctcttagaaa acgaaaagtc gtgagttgcg
    40801 gtatagtttt atgtataaaa acgaggagaa ttttatgagc aacgtcatta tcaaagcaac
    40861 aaacgaccaa cttagtcaaa tgaaagcata ctatcgctct tatttgttaa ataaacagat
    40921 tccttatact agctttgctg caaaaaaaga cgggacgacc attaccgctt atacatccgg
    40981 aaaagtgatg tttcaaggta cgaatgcagc aatagaagct gataaatggg gggaggcaca
    41041 ggataaacaa gccaagccag caaaaacagc ctcttctttg ccaaaaaata tcagtcaatt
    41101 atccgtttta ggtagtgatg aggtcggaaa tggcagctat ttcggtccgc taacagtatg
    41161 tgctgcttat gtgaaacgtg atcagcttgc agaattacgt aaacttggtg ttagagattc
    41221 aaaagagtta aaagatagcc aaatcatcca gcttgctgac atcttaaaaa aaaccatccc
    41281 ctataaactg cttgtcttat ctccagaaaa atacaatgag atccaaccaa aatacaatgc
    41341 cgttcggatg aaagtcgctt tgcacaatca agcgatccat ttgctgctaa aaaaaatcga
    41401 gccagaaaaa ccagaagcga ttttgatcga tcagtttacg ccggaagcaa attttaaaaa
    41461 atatgcgcgt ttagaaaaaa atcaattaca agaaaaactg tacttcgtta ctaaaggaga
    41521 acaatatcat ttagctgttg ctgctgcttc tatcatcagt cgtgccagct ttttggaaga
    41581 gttagacaaa gcatcagctg aagctggaat cacccttccg tcaggagctg gaacgaaatc
    41641 tgaccaagta gcggcaaaac tattggagaa aggcggcatg cctatgcttg aaaaatatgc
    41701 caaacttcat tttgccaata cagaaaaagc tttgaagcta ctaaaaaaaa aatggaactg
    41761 tgacaaaagt catgtcacag tcccttgttc cgaataaacg gtgttcaatc atctgatcct
    41821 cggtattagc tcaacaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat
    41881 actcagaaca aaacgttgat ttcacaacct ttttcttaaa cggtgttctc atgccaactc
    41941 ctcggtcaca agtcacaata gtcatcaaac atgagaagct gttttttgaa attgctcctt
    42001 gtgactcaga gttgaacggc aggctcacga cctcttcctt aaacggtgtt taaaagcttg
    42061 catctgcgat aataagcccg gccaaacgaa aaatatgagg agctattttt gtttggctgg
    42121 gcttatttat tactgaaaat aagtggtcaa tgcttgtgtc aaacttgcag caatctgctg
    42181 ctgataactg tctgttacta attcctttaa atcactatca ttattcatat aacccatctc
    42241 gattaaaata gaaggttgaa gattttctcg aagaacttga tagttcccag tggagattcc
    42301 tcttgaatcc agaggcagat cagtgaagga gtcagcaatt gtctgtgcta atgtttcctc
    42361 ttgtccatag taataatatg tcgtgattcc agttgcttca ttcgcatatt ccgtagagtc
    42421 tgcgtgtaaa ctgataaaga catctgcctt tttttcttca ctgtagtcgc atatatctcc
    42481 aagctgtact aattcatctg tagtatgggt caaataaacc gttgctccag catcctctag
    42541 cgcctttttt acttttatgg ctgtacttaa tgttatatct ttttcttctg tttgttcatc
    42601 attgcttaat gccccagtat catttccgcc atgcccagga tctaatacaa tcactgcatc
    42661 agatagctct tccggttttg tttgtatatt ctttgtgact ttggttccat cttcgagggc
    42721 taaatatcca gtatagccat catttgtctt gatctgataa gcattttttg tttcagaaag
    42781 aaactccgcc agctctccgc cgtttatagt tgcaagtggg gtagttagcg agtcatctgt
    42841 atatagtgta cggtcatttt ttccgatttt gattaatgtc tcttgttctg tatcatcaaa
    42901 tgattccttt gtctttgtaa ccgtactgtt cgtactgctg accgtttgtc caactatctt
    42961 tttttgacca aaaacatcta tttgaaaaca gataataatg ataatgattg caaatacaaa
    43021 agctccaatt atttttttca ttcttgactc tcacctcgta gatccagact aagggccaaa
    43081 acttaaaatc agcttaagta agcaaaaaag gggctgtaaa agatataacc gtagaaaata
    43141 acattactgc taaaatgatt tttttcattt ttggcatatc ctgaaatctc cttttgacta
    43201 ttcgtggtga ttcatctacc caataaatag tttccttcat tctacaaaca attaatttct
    43261 tcgttattaa attaaaacac ttcctggaac accgacataa taaataaaaa tataccctga
    43321 taatcatgag agaataacaa atttgtattt atgaaaaact acctatataa agaagcaaga
    43381 tataacgatc ttcgtcatat cttgcttctt cacctaacta ttttctccct atataacagc
    43441 tttacgcttc tcctaataca ccattcaaat ataaatcgat caattgcgcc acatttagcg
    43501 gttcttccac aggtcttttc gaccgagtga tgtaaggtgc taacactaag aaaaaatgcc
    43561 ttgctgcgat ttcttttgaa atcccatttc ttagcacaga ttggtttgct gaaaagattt
    43621 cttcaaaagg ttcacgataa tttttcttcc atagaaagaa aagatgatct cttgtttcct
    43681 ttgaaagatt gctttcaaga tcgtgaagca tcagaaacaa atcgaccaga tgtgtctcaa
    43741 tcaaaaactg cgccattttt gtcaaggtta cttgaaatcc cgtttcgctt gtttgaacga
    43801 tttctctcaa agcttttcca acatctttta agtggttttc taaaactgcc gtatatagtt
    43861 tttctttatc accgaaataa tgatagagat tgggctgggt gatcccaact ttttgtgcga
    43921 tttctcttgt tgaagtgccg agatatccct tggacataaa caagtcagtc gcttctttca
    43981 aaattttttg ctgtgtatct ttgttgtatc gatggaccat ttttccacct gctttttggt
    44041 tattcttttt ctttcaatac accatcttcc atcataaata ttctatcgca tttgtcaatc
    44101 agccgtgtat cgtgagtaac catgatcgtt gctttatttt tgtcttttga ttcttttgcc
    44161 aagatatcga ctacttctcg tgccttttct gaatccaaac tagcagttgg ttcatctgct
    44221 agaataatgg acggatcatt gtacaatgca cgagcgattg cgaccctttg ccgttctcca
    44281 ccagatagtt cttctggata cttgttgatc aacttatcca cgccaagctg ctcaaataat
    44341 tcgtttgctt ctttttgacg atgttcatgt ttaatcttat ctactaataa taactgcttt
    44401 tttactgtca gaaaaggaac cagattggaa gcttgcaaaa taaagccaat ttctttaaaa
    44461 cgtagtttaa cgcgttcgct ttctttttga ttactgaatt cttgatcatt gataaataca
    44521 gagccactgc taggcgtctg caatccgcct gccaaagtca aaaaagtact tttacctgaa
    44581 ccagaaggac cgatgattgc aacaaattcc cctttatcga tactcatggt tgtttctttc
    44641 aatgctttaa ctacttggtc gccatcttgg aaactttttt ctacttcttt aaattctatt
    44701 gctttcatgt tcttcctcct tcttatccaa ttgccttcaa cggatcgatt tttacgattg
    44761 tccgaacaga aaatagtgcg cctaacacag ctactactaa catcaacaag ctaatgacgc
    44821 caaaaaacag ccaattgctt tggaatggaa ccgcatcagg tagaacaagg gatgttccca
    44881 ctgttcctaa tagaccaaga agaataccaa caaatgataa aatgaatgtt tgtgccacta
    44941 cagaacgcgc aataaatcct cctgtgatcc cttgtgcttt cattacacca aagatattaa
    45001 tcttttgcat cgtcagtaca taaatgaaaa tcccgatcac aatagccgca ataacgatca
    45061 aaaatccgat cataaatcca aaagtcaata cttgcgcatt atatccaggt aattcattga
    45121 tgaatttaga aattttgatc tgttctaaat cttcaggtaa attactgatt ttacctctga
    45181 caactattgc gttaatccgt gcattttcac ttgtatcttc tttttcgaag cggatttctt
    45241 ggtaagcatt gatggttgta tagagaactg gtgagacatt gaatttggca tgatccgtaa
    45301 agccagttag tttaaacgtc ttgtcactac cagataactt aactgtatct cctatggcta
    45361 gaccatattc ttctttcaaa ctaatatcgc ctactgcctc atcatcgtta tcaaaagctt
    45421 ttccttcaac aagattgggc atgatgaatt gatttttatc gataccgaag aagctaacat
    45481 tgatttttcc ttcttctgta ctgtctttac tagtcacgac tccaggtgtt tgggcaagat
    45541 acgccacttt cccaccttcg acttcttctg ctatcttctt agtaatcatg gacatcccta
    45601 gatttgaatt tgcatctttc gaaagcacaa tcgaatctgc ctcccacttg tcgactgctg
    45661 ttcgattatc ttgagctaag ccataagcta acccagtcaa aaaaaatacc aggtaggcga
    45721 tcaaaaacat cacccctgca actaacgcat aacgtaactt tgaatgcata atttcattta
    45781 atgctaaaaa catttaactc ctcctcctga aataatcatt ttatacaaca attttatcac
    45841 ttgataaatt tttggcgaat attatgctca aaaaattctt ccgctatttc attatatgaa
    45901 ttttttattc tcgattgatt aatatttatt atatccccac acaataaaag accgtgatat
    45961 ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta ctttaaaagc
    46021 taactcattg aggtagtgaa aacgagaggt gtattcagac agaaatccaa aatcctcaac
    46081 tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt tcaaatctga
    46141 taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat ccggatggta
    46201 gatcagcttt gcatttactg ttttgttttc ttcgtcgtat tgtggaaata gatcaaattc
    46261 tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg tcagccctac
    46321 agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga ggtcgtaagc
    46381 ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag ctcgagtaac
    46441 taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga tatctccaat
    46501 cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag tctcatcctc
    46561 atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg cagaaaaaat
    46621 gaccatatct gcctttattg tttgagacag aagttgtacg gatactactt tctcttctga
    46681 ttttttccaa tcgatcaaga attcatttgg ataaaaattt atttgcttag tttgcagctg
    46741 attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat tcggcatata
    46801 atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt ccaatccaat
    46861 gtaaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa gtacttgctt
    46921 agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt tatacaaggt
    46981 gacaggagag gaacctgtcg ctacgattaa tttatcatag gttatggtct cgttttttcc
    47041 ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta ctctccattc
    47101 ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa cttcagctaa
    47161 actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc ctagtccatc
    47221 tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc ttagatttag
    47281 ggcagctgtg atgccttcat gggaagcacc aatgatcacg attttcatgt tttattcatc
    47341 cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata ggggaaatca
    47401 taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga cgggtactct
    47461 ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc gactaaatca
    47521 tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc cgccattagc
    47581 ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt gatcagcgtc
    47641 cgtaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc gtctacttca
    47701 gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac caggaattgt
    47761 ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat attcatccat
    47821 cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat cttttgcgaa
    47881 atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt aaacgttcga
    47941 ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc ctggacatgc
    48001 gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac caacgtttca
    48061 ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt atggaggtcc
    48121 aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc tggtttggat
    48181 tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa tttcaatcgt
    48241 ccttcttctg taatagcaaa actaaactct ttttgcagca gatctaaaaa agtgtttcct
    48301 gataaatcac tgtattcttt tttgttttca agatagggac gtccaaacca aaataattgg
    48361 aaataaaaat aacgaatttg actttctgga ccggtcagcc gcccgctttt tattttcaga
    48421 tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt agccgaactg
    48481 atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa cagataaaga
    48541 agtatctgat attttgtaga atagcgcaag taacaatgat aataaacatc gactgctgct
    48601 atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc caattgtttg
    48661 atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc ccgatcaact
    48721 gataagcggg taagtacgtt attaacggaa acctctcctt tttcctctaa taaaagttgg
    48781 aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc caagtccact
    48841 ccttcaagca tttctgattt taacagcaga agtttccttc tcatccttga aaaagaggat
    48901 ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg gcccagctgt
    48961 cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg tactgctatt
    49021 tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc ctttagctgt
    49081 tccatcactc tcataaatca acgtgttttg accttgctga atcaattgct ttgatccaga
    49141 atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct taaataaaga
    49201 agtcaatgta cctgtcagtt gccaaggaga cgtgcttgga agccgagtat cccgcacatc
    49261 aaaagaaaaa tcgccttttg attcataata acttgtcttc aacggtttgg ggagatcgtt
    49321 accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg ggacaagaat
    49381 tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg ctaatgtaat
    49441 attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtaaag aagtatctac
    49501 agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc tcaatagatc
    49561 tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac catttaattt
    49621 taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt ttttgtcggt
    49681 tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg tatctcctga
    49741 tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta gctgactatc
    49801 tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga ttccagcttg
    49861 ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat cgtccaccaa
    49921 gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag tagctgtatt
    49981 tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc ctgtcgctaa
    50041 tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta gttcagttgc
    50101 tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc actgactatc
    50161 ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca atacctttaa
    50221 agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga tattggctgt
    50281 ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt gtgtaacgtt
    50341 ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact gatcaaaacg
    50401 tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa ggttctggtt
    50461 atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg ttgaatcact
    50521 tggtgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg catatgctcc
    50581 tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg gagctaaaat
    50641 cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa cagaaaaaac
    50701 ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt ttggactcgt
    50761 atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat caaattgaag
    50821 cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat ctgtcaatag
    50881 agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg ctgataataa
    50941 acttccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt tgttccagtc
    51001 aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga tcgccacaaa
    51061 tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt tcacatggct
    51121 ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat cgttcaaata
    51181 agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct tcccgccatt
    51241 gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg gatcataaga
    51301 aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa atatacttaa
    51361 aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt gtagcaacat
    51421 gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc cataattcta
    51481 taccaatgat caaataaaga aaacggatct tttgttcatt cgtctccggg aatacgccat
    51541 gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg ccattgactg
    51601 aaccacttgg atgcggaatt ggacttagat cataaaatgt aatcgtacct gaacttttgg
    51661 tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata atccctccca
    51721 ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt tttctttttc
    51781 ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc aaccaatcct
    51841 gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc atttactttt
    51901 tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt actgtcgcct
    51961 gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc agctaaatca
    52021 agattcaagt gaaaattcga atttggtgca actaaaaatg cattagacgt attttcaata
    52081 taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat aatcctaggc
    52141 tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc caaatctcct
    52201 gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac gactgcgaga
    52261 ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt ttcagcttct
    52321 tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt tgctgggatt
    52381 ttcacttcta taggtacagt tgtttctgca tgtgctgata cattgatttc ctttttcgca
    52441 atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg gtttttttga
    52501 ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac ctcaaatgtt
    52561 cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg ttccggtgcc
    52621 actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa taccggtttg
    52681 attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat gaaaaataga
    52741 cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt ccaccacatc
    52801 ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc gcagtttgtt
    52861 gaatttattt catactctat gctactgggc cagccacgag gttccaagtc aaatcagccg
    52921 tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga tcataaacag
    52981 aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc gctgcacttg
    53041 ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta tctgacgtac
    53101 ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt gaaccgttta
    53161 caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct gcacctacac
    53221 ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct acttgatacg
    53281 tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcgggaact acatccagtg
    53341 ttaaaggacc tgtctggcca gttgctggat tttccggatc tgctggctct actggttttg
    53401 aaggatcagt agggttaaga ggctccacgg tatttgttgg ttcagtaaag gtaatatctc
    53461 cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct agaagaagtg
    53521 ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc tttttcttag
    53581 tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc cgcttacttg
    53641 aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta catgaatatt
    53701 cttccttttt tattaagttt cgtttgtcaa attaagttag acaaatcatc ttttgtgacg
    53761 taactcaaaa atacttccat tggtgtccga tagtgtaatg attttctagg aatatgattt
    53821 cgtttcgatg ccaccgattg gataaaggtt tcagatacac cattaaaatc catagcttta
    53881 tacaaaccat ctttacgtaa taagccattt gaatgttcat tcaatcctcg ttgagaaggt
    53941 gttcctggat ccgcaaaata aattgaaata tcgttggtat tactaatctt tttccaattt
    54001 gagaattctt ttccacaatc aaatgtgatg gatttaaata agttttttgg gacacaacga
    54061 aaccattgat tcagcctatt ttcaatgtct ttagcctgtc tgccttcggg tttcaaggta
    54121 atgatgactt tagatagccg ttcaactaat gtgatgaccg cgcttttatg ttttccaccc
    54181 acgatagtgt caccttccag gtgaccaaat tcatgatcaa actgggtata gtctgtcttt
    54241 ctgtctcgga ttgaccggcg aaacccttgt tttcctcttt tttctttatg accgtttggt
    54301 tttctttttc ccttcattgg taaatcgtga gtgtcaaagg ttttatcttt aaacattcga
    54361 tacagtgtgc gtaccgaaca agcaatagga aactctgctc gaccaataag gacatcagga
    54421 gcccaccctt gaacaacctt tttttgaatg tattcctgtt gctcatcagg taaaacaatc
    54481 ggatgtcttc cgcagttacg tttgtttttc ttatattgtt tatagtagct cattgcgctt
    54541 ccaccagatt taagaaaacg atagacctta taaatagttt gtcgagaacg ttgtaacaga
    54601 ttggctactt ttgaaacagg ttgtgattga ttgaaataag cttctatcat tacgagttca
    54661 gttggtgtaa gatggttata ggtcatttgt actcactcct tataattctt tcgtcggaac
    54721 tattttgagt gtagcacaaa tgactttttt tagttgtcta gcttaatttt acaatcggcg
    54781 aagtatgaaa aaatatttca attttatttt ttcgtttcaa attattctat tagtgacatc
    54841 cttgaactat aaaaatagga gtacggcatc atctgccgca ctcctatttt tttcgtcatt
    54901 tgtttcttaa ataaaacgat tctcacgcaa ttttattttt ttgatatctt ctactgtttg
    54961 tgttccagct aattgcatga ccatttccaa ttctgtttta aagaaatcaa acacttgatg
    55021 gactcctgta cttcctccta aagacaatcc ataaataacg ggtctgccaa tagcaacaag
    55081 atctgcacct gatgcgattg ctttgaaaac atgctgtccg cgtcgtacac cgctgtcaaa
    55141 cacgattggt acacgtttat ctactgcttc tgccacatat tgcaacgaat caaacgctgc
    55201 tggaccacca tctagttgac ggccaccatg attagatacc cagattccgc cagcaccaga
    55261 ttctaatgaa cgatagacat cttcttctga ctggacacct ttcacataga ctggcaaatc
    55321 agaatgagca gcaataaatt ctacatcttt tggactaagc ttctgtttgg aagatttgta
    55381 cacagcatcc atcgtttggc ctactcctga ttgataagcc tgaacgatcg gcattggcag
    55441 tgggaaggta aacccatttc tgcggtctgt ttcacggttt ccgccaactg ttgcatccgc
    55501 tgtcaatacg attgcttttg ctccattacg cttggccact tcaagaatat ccaaattgat
    55561 cccatcatct ttgctcatat aaaattgaaa ccattgcggt gctttctccc cgcctgcttc
    55621 tcttatctct tctaatgtac aagaagcgta agaacttgct gtatagatcg tcccaaaatc
    55681 tgctacacct tttgcagaag ctttctccgc cttcacatga gctaagccat gagcagcaac
    55741 tggtgccatg atgattggtg cagtcagcgt ttctttgtca aaatgtgtag ttgtatcggg
    55801 taattctaca tctcttaata catgaggaat aatcagctga tgattgaatg cccgttcatt
    55861 ttcttgataa gtaaataagt cgccagcacc actgctgatg taaccataac caccagctgg
    55921 aatcacttgt gaagcggcta tttctaaatc ttccatattg ataaaatcca ctattccttc
    55981 atttgtacct gcttgatacg ttttttccat ttcaatgcct tccctctata aaaaattgaa
    56041 tgaataatat gatagcgttt acacacttta tattactctt ccgtattgat acaggcaaat
    56101 aatctgttta catttcagtc attccagttg ttactactct gaaagaaatg tttagaacac
    56161 aaatagataa aaaacgatgg gattttgaca aaagtcgtgt caaagtccct tttacgaaat
    56221 actgtggatg cctattgaaa taagtgcggg ttgtttcttt gtatttcaat cgtttcttgc
    56281 cattcttcgt ttaattttgg attaccgttt tcttccttga acgcataagg tcccaatcca
    56341 aaaagctcag ttagtatttg attcacgatt ccatatagct cggtgtgatc ggacacatga
    56401 ttattgaaga tcatctcatt atcaccacgt ttgaatctca cattcgggct ctcatgatga
    56461 acctctatgc agttttcccc tttttttaac tgacggattt ctgaaaacat ctcataaata
    56521 tcttctccaa taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcaac
    56581 tcagagatta tcgctttgtt ggcaccaagc tttaatgtat agcttgttgc ttctactacg
    56641 ccgcatgcat tgcaaccacc ttctgatagt tcgctttttc tgatcaattg aacttctttc
    56701 attttctgtt cctcctctta gttttctact tatgtatttt ttatcacctg acgtaagtgt
    56761 attttatcgt cacgatcacg tgatttattc ttcagagatt ct
//
LOCUS       DASHWH010000009        56543 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.009, whole genome shotgun sequence.
ACCESSION   DASHWH010000009 DASHWH010000000
VERSION     DASHWH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.009"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(135..680)
                     /locus_tag="U2R41_000557"
     CDS             complement(135..680)
                     /locus_tag="U2R41_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8887991.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(692..1384)
                     /locus_tag="U2R41_000558"
     CDS             complement(692..1384)
                     /locus_tag="U2R41_000558"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEM8887992.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1403..1687)
                     /locus_tag="U2R41_000559"
     CDS             complement(1403..1687)
                     /locus_tag="U2R41_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8887993.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1700..2263)
                     /locus_tag="U2R41_000560"
     CDS             complement(1700..2263)
                     /locus_tag="U2R41_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8887994.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2491..3153
                     /locus_tag="U2R41_000561"
     CDS             2491..3153
                     /locus_tag="U2R41_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEM8887995.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3277..4473
                     /locus_tag="U2R41_000562"
     CDS             3277..4473
                     /locus_tag="U2R41_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEM8887996.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4574..5950)
                     /locus_tag="U2R41_000563"
     CDS             complement(4574..5950)
                     /locus_tag="U2R41_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8887997.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5987..6853)
                     /locus_tag="U2R41_000564"
     CDS             complement(5987..6853)
                     /locus_tag="U2R41_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEM8887998.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6942..7967)
                     /locus_tag="U2R41_000565"
     CDS             complement(6942..7967)
                     /locus_tag="U2R41_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8887999.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7985..8659)
                     /locus_tag="U2R41_000566"
     CDS             complement(7985..8659)
                     /locus_tag="U2R41_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888000.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8843..9736)
                     /locus_tag="U2R41_000567"
     CDS             complement(8843..9736)
                     /locus_tag="U2R41_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8888001.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9810..11507)
                     /locus_tag="U2R41_000568"
     CDS             complement(9810..11507)
                     /locus_tag="U2R41_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8888002.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11500..12813)
                     /locus_tag="U2R41_000569"
     CDS             complement(11500..12813)
                     /locus_tag="U2R41_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEM8888003.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12813..16850)
                     /locus_tag="U2R41_000570"
     CDS             complement(12813..16850)
                     /locus_tag="U2R41_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM8888004.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16863..17321)
                     /locus_tag="U2R41_000571"
     CDS             complement(16863..17321)
                     /locus_tag="U2R41_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8888005.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17619..18137)
                     /locus_tag="U2R41_000572"
     CDS             complement(17619..18137)
                     /locus_tag="U2R41_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM8888006.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18139..18756)
                     /locus_tag="U2R41_000573"
     CDS             complement(18139..18756)
                     /locus_tag="U2R41_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEM8888007.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18753..19616)
                     /locus_tag="U2R41_000574"
     CDS             complement(18753..19616)
                     /locus_tag="U2R41_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8888008.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19603..20514)
                     /locus_tag="U2R41_000575"
     CDS             complement(19603..20514)
                     /locus_tag="U2R41_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8888009.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20555..21889)
                     /locus_tag="U2R41_000576"
     CDS             complement(20555..21889)
                     /locus_tag="U2R41_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888010.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22097..22927
                     /locus_tag="U2R41_000577"
     CDS             22097..22927
                     /locus_tag="U2R41_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEM8888011.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23171..23489)
                     /locus_tag="U2R41_000578"
                     /pseudo
     CDS             complement(23171..23489)
                     /locus_tag="U2R41_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23611..23805)
                     /locus_tag="U2R41_000579"
     CDS             complement(23611..23805)
                     /locus_tag="U2R41_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888012.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24310..24495)
                     /locus_tag="U2R41_000580"
     CDS             complement(24310..24495)
                     /locus_tag="U2R41_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888013.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25345..25686)
                     /locus_tag="U2R41_000581"
     CDS             complement(25345..25686)
                     /locus_tag="U2R41_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8888014.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25686..25916)
                     /locus_tag="U2R41_000582"
     CDS             complement(25686..25916)
                     /locus_tag="U2R41_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8888015.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26203..26541
                     /locus_tag="U2R41_000583"
     CDS             26203..26541
                     /locus_tag="U2R41_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888016.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26746..26985)
                     /locus_tag="U2R41_000584"
     CDS             complement(26746..26985)
                     /locus_tag="U2R41_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM8888017.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26961..27308)
                     /locus_tag="U2R41_000585"
     CDS             complement(26961..27308)
                     /locus_tag="U2R41_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM8888018.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(28040..28621)
                     /locus_tag="U2R41_000586"
     CDS             complement(28040..28621)
                     /locus_tag="U2R41_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8888019.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28804..29430)
                     /locus_tag="U2R41_000587"
     CDS             complement(28804..29430)
                     /locus_tag="U2R41_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8888020.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29452..30783)
                     /locus_tag="U2R41_000588"
     CDS             complement(29452..30783)
                     /locus_tag="U2R41_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEM8888021.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30938..31339)
                     /locus_tag="U2R41_000589"
     CDS             complement(30938..31339)
                     /locus_tag="U2R41_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM8888022.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31425..31631)
                     /locus_tag="U2R41_000590"
     CDS             complement(31425..31631)
                     /locus_tag="U2R41_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888023.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31726..33228)
                     /locus_tag="U2R41_000591"
     CDS             complement(31726..33228)
                     /locus_tag="U2R41_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEM8888024.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33246..33701)
                     /gene="argR"
                     /locus_tag="U2R41_000592"
     CDS             complement(33246..33701)
                     /gene="argR"
                     /locus_tag="U2R41_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM8888025.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33799..34416)
                     /locus_tag="U2R41_000593"
     CDS             complement(33799..34416)
                     /locus_tag="U2R41_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEM8888026.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34637..36010
                     /locus_tag="U2R41_000594"
     CDS             34637..36010
                     /locus_tag="U2R41_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8888027.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36086..37198
                     /locus_tag="U2R41_000595"
     CDS             36086..37198
                     /locus_tag="U2R41_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEM8888028.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37270..38907)
                     /locus_tag="U2R41_000596"
     CDS             complement(37270..38907)
                     /locus_tag="U2R41_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEM8888029.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39075..39683)
                     /locus_tag="U2R41_000597"
     CDS             complement(39075..39683)
                     /locus_tag="U2R41_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8888030.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(40067..41218)
                     /gene="queG"
                     /locus_tag="U2R41_000598"
     CDS             complement(40067..41218)
                     /gene="queG"
                     /locus_tag="U2R41_000598"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEM8888031.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41237..41989)
                     /locus_tag="U2R41_000599"
     CDS             complement(41237..41989)
                     /locus_tag="U2R41_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888032.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42130..43080)
                     /locus_tag="U2R41_000600"
     CDS             complement(42130..43080)
                     /locus_tag="U2R41_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM8888033.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(43081..43920)
                     /locus_tag="U2R41_000601"
     CDS             complement(43081..43920)
                     /locus_tag="U2R41_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8888034.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(43917..44663)
                     /locus_tag="U2R41_000602"
     CDS             complement(43917..44663)
                     /locus_tag="U2R41_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8888035.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            44901..46271
                     /locus_tag="U2R41_000603"
     CDS             44901..46271
                     /locus_tag="U2R41_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8888036.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46326..47222)
                     /locus_tag="U2R41_000604"
     CDS             complement(46326..47222)
                     /locus_tag="U2R41_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8888037.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47336..47506)
                     /locus_tag="U2R41_000605"
     CDS             complement(47336..47506)
                     /locus_tag="U2R41_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888038.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47503..48180)
                     /locus_tag="U2R41_000606"
     CDS             complement(47503..48180)
                     /locus_tag="U2R41_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8888039.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48271..49176)
                     /locus_tag="U2R41_000607"
     CDS             complement(48271..49176)
                     /locus_tag="U2R41_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8888040.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIRVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49351..50805
                     /locus_tag="U2R41_000608"
     CDS             49351..50805
                     /locus_tag="U2R41_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8888041.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGVIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            50821..51852
                     /locus_tag="U2R41_000609"
     CDS             50821..51852
                     /locus_tag="U2R41_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8888042.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            51859..53046
                     /locus_tag="U2R41_000610"
     CDS             51859..53046
                     /locus_tag="U2R41_000610"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEM8888043.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53202..54584
                     /locus_tag="U2R41_000611"
     CDS             53202..54584
                     /locus_tag="U2R41_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8888044.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54711..54941
                     /locus_tag="U2R41_000612"
     CDS             54711..54941
                     /locus_tag="U2R41_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888045.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(55141..55809)
                     /locus_tag="U2R41_000613"
     CDS             complement(55141..55809)
                     /locus_tag="U2R41_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEM8888046.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(55998..>56543)
                     /locus_tag="U2R41_000614"
     CDS             complement(55998..>56543)
                     /locus_tag="U2R41_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEM8888047.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT        16278 a        11797 c        10017 g        18451 t
ORIGIN      
        1 aagagattta ttaacggaaa aagaataagt ttttttaaaa taacgtataa aaaagagcat
       61 aaaacaaagt cgtttgtttt atgctcttct tttattattt tgtgcttttt attttatcaa
      121 atcactttgt ttttttataa aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc
      181 cattcatgtc caacgggatc gaaacttgcc acggaatctt tgaaaatatg gagtttgtat
      241 cctagattat acgcatcgac tgctgtatgc aacacacaga tatctgtaca tacgccggtc
      301 aaataaatat ctgtgatctg gcgttctcgc aaacggatgt ctagatctgt tccactaaaa
      361 gcggaataat gccgtttgtc tatccaataa acattttcct cttgctcata gtcttggtat
      421 aaacttttta aagagccgta caattcccgt ccacccgttc ctatcacatt atgcggagga
      481 aataagcggt tctccggatg aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg
      541 acaaaatcct tttgatcaat aaacttcttt gtatgagcag tcagcttttt ttcaattact
      601 tgtcctgctg tcccagtagt cagctttcca tcctctgcaa caaaatcata tgtgtagtca
      661 atcgagatca atgcgttcat cttagtcctc cttatacgag atgtttgaca aattcgatca
      721 ccatttctgc tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg
      781 agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg aactgacgag
      841 ctgtttgtcc aacagcggct ccttccattt cgcaagctaa tgcttcaggg aaattcgtta
      901 agatttcttt gattttatct ggcgagtcga caaatgtatc acctgtaacg atcaatcctt
      961 cttttgcgtt caaattcgtt gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc
     1021 ttgcttcata atatagaggc attcctggta attgtcctgg gttgtagcca aatcctgttg
     1081 catctgcatc aaaataagct actttgtctg atataacgat gtcacctact tgtaagcctt
     1141 caccgattcc gccagctgaa cctgtattga tcaccatgtt cacgccgtat tgttggatca
     1201 acagacttgt ggtaatagat gctaagactt ttcctatacc ggaacgtacg acgatcactt
     1261 cgtggttacc taatgaacct gaaataaata aagcgcctgc gcgttcccat gataatggtt
     1321 cgcttaggtt ctctctcaaa atttttactt cttcttccat tgcgccgata ataccgattt
     1381 tcatcttttt aattccttct ttctatataa atgcgattgc taaaaaaacc acacataaca
     1441 ttaggataac aataataaga gatttcatca aaaaagagtt ccatttggtc gtcttttccc
     1501 gttcgctgat ccttgttttc gtgattggtt tattcttttt gctttcttta cgataaaagc
     1561 tggcaatctt tttttcttct tgctgataag ctgtttctgc ttttcgctgt ttttttaatg
     1621 actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt
     1681 tggccataag ccgatccctc tactttcctt tggtcaatag ttcccagtat tgaatcgcat
     1741 aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag
     1801 ttagatgata caactccagt acttcgtctt catcttgtgc taaaggattc tcgaccttct
     1861 ctaccccttg tgcatgataa atatgaagga cttcatttgc aaaacctggt gaaagataca
     1921 tcgaagtaag atggctcagc gacttcgcac gatagccagt ttcttcttct aattctctag
     1981 ctgctgtcat ttcgggattt tgtccttctc ctggatcgat ttttccagct ggaatctcca
     2041 aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag aagcagacga tcttgttcgt
     2101 caaaagcaat gatcccgaca ccgccaggat gaaataccag ttctctcttc gaagttcctc
     2161 catctggcaa acgaacttcg tctacagcaa catcaataat ttttccgcta tagatttctt
     2221 tgcgactgat tgttttttct tcaaattgct cattgttcag catatcttct cctgctttct
     2281 ttgtaatcat aactgctttt ttttcatttt tcaatttttc tcaaaaactc cttattagtt
     2341 taccatagat aaaccttaga ttttctctaa atatagaaaa tccttcttta gtccgatact
     2401 ctttctagta gtccgtatgc taaaataagt atgtaatcga ataacgaaaa gaattcaata
     2461 ctcaacgata aatgacatgg agggtttatt atgataaaac gaaattggcc gtggcttcta
     2521 gcattggtct tgctatttct ctttgtccga aacaatagtt tttcttttgt tctaggactt
     2581 ttattgatag gtgtagtttt agttttgtgg ggactctttg gttcgcgcag gaaaaaggga
     2641 aaacaagaag aaattcctgc actgactgac gaacttgaaa ctcactatga aaaaagcggt
     2701 atgacagcga atgaaatttc attttttcga caaacaatga atcaaacaaa aaatgagatc
     2761 aatcaattgc aaatgaatat gcagcagaca gccaaactta aagcaatcga tctccgccat
     2821 gatccagtca aagcagcgaa agctttattc aaagaattgg tgaaagaacc aaaccgactg
     2881 catgaagcaa gtcagtttct ttatacgcat ctgccgaatc tagttgattt gacaaataag
     2941 tataatgaga tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa actagaagaa
     3001 agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac aagattatca aaaattcgtt
     3061 gctgaggatc tagatgatat cgacgtagag atatctgttg cgaaacagag cttaaagcgt
     3121 gataataaat atgaaccaga acaaaaggat taactctttt gttcataaaa gaagactcgg
     3181 aacattcaga tgatgtctgg ctgtttcgag tactttttct aaagtagaga cattcatgta
     3241 agaatcgaga acagattcaa caaggaggaa ttttttatgg atcataaacc gactgaaaca
     3301 aaagatgtta caccagttaa cgatacactt gaagatttat tgaacaaccc gttttcaact
     3361 ccaacagata cgctgaccac ttcacaacaa gcggagattg atcagctcca agagcaacag
     3421 acagctgctc gtttaattga caaactaccg gcggaacgcc aagaacaagc taaacagcta
     3481 gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca gctacggatc ggctgcccaa
     3541 gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca agatattggt
     3601 cctgtaggag attccttaac tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa
     3661 ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc
     3721 tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa
     3781 ttagataaag aaaaagacgg tctgctaaaa gataatctga tgttagaaca gctttaccag
     3841 aaaaacaaag actactttga tgcactgaat atttatatcg ctgctggaga attgaaaatg
     3901 gaagaacttc aaacaacgat cattccagaa gcgatgaaaa gagcagaaga aactggcgat
     3961 cagatggatg ttcagattgc caatgattac acgcaattcc tagatcgtct ggataaacgt
     4021 acgcatgatc ttcgcttagc tagacagatc acgatccagc aagcaccaca aatccgtttg
     4081 atccaaaaca caaatcaggc cttggctgaa aagatccaag catctatcgc tacagccatt
     4141 ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa agatgcagta
     4201 actgctcaac gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg
     4261 ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag
     4321 acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc aagagacatt gcgtatccaa
     4381 aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc ttggacacat ggaagaagat
     4441 ctgaaacaaa aactgttgga tttaacccaa tagatcacct ttcgcttgtt actaaaacaa
     4501 acgcccaaca acagtttatt acaactgttg atgggcgttt ttcttatctt tcttctaact
     4561 tctccattgg agtctatttc tcattcatct tgctgtgtcc atagccatag ccaaaataaa
     4621 tgatcagtcc aataaccatt acgataccga acacgatcca tgtcacagtt tgcagacgaa
     4681 gcatcaatac aatacaagtc aagatggaga cgattggcaa tagcggaacg aatggcactt
     4741 tgaattctcc agcttttggt ttgccaaaca tttttctcaa cttcagtaag cctagactaa
     4801 caagcatcaa ataggcgagc gccatgatat tcgttagttc cgctaataca ttcaatggga
     4861 aaaatccggc aaagaataca gctgccacac cagcggcata agtcgctttt ttcggtgtac
     4921 gatgcttttg atcgatttct gtcataaact taggaagtaa gccatccttg ctgacagcaa
     4981 acagtaaacg agcaagtgaa tacatcatcg agatcgttac cgtcagtagc gttaaaattg
     5041 cccctacagc gatcaccgcg cccactgttc catgtcctac atacctcatt gcaaaagcca
     5101 ctgggtcatt gactcctagt tcagtatacg gaacgattcc tgtcaagatc agcgtcacga
     5161 tgatgtaaag gacagtagca atcagtatcg aaccaatgat tcctcttggc acatcttttt
     5221 gcgggttttt gacttcctcg gctgccattg caacggcatc aaatcccaag aaagcaaaaa
     5281 acacaagagc tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt
     5341 tatcaggttt aacgaaaaat attcctacaa tgataaacaa cgcgatgata ccaaatttga
     5401 catataccat ggcattattc aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag
     5461 tgacgaatat cactacgagc accgcaatca agtcaatgta tgtaccattt tccggattgt
     5521 acccgccagt taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat
     5581 agccagacca tccggaagca acagaggaaa ccgctagtaa aaattccccg atcaacaacc
     5641 accctgtcag ccaggcagca aattcaccaa atacaacata cagataggca tatggcccac
     5701 caatcactgg cactcgcgaa gcaaattctg cgaaacttag tccagacaaa atgacaacga
     5761 ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc attttgattg gccgcaaccc
     5821 ctgtcaccac aaatattccc gtaccgatga tcgcaccaat tccaagcatg atcaaatcag
     5881 acgtttttag ttcttttttc atctcactcg tatgattgag attgatctct ttcttacgaa
     5941 acaaatccat tgaagttcct ccccatttta ggttggaaaa cagcgattaa actgttttca
     6001 tataccctaa aaggatacca cctaaaatga ctaaaaggca accaacaata acataaatga
     6061 gctctttttt cgtttttctt tcgccaagga tgaaaatccc acctaaagta gagataatga
     6121 tccccatttg tgaaagtgag aagccaacag ctaaaccaag tgatttgact gttagaagca
     6181 tgcagatgtt tcctaagccc cataataatc cacagatcat gtttttccac acaaactgat
     6241 cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg atactttgag
     6301 gtaagataat tgacatcgca tccaaattcg cccagttgac gatgatggta tatacaccat
     6361 aaccaatcgt agagaagatc aatgctctaa aacccttacc aaaatcaagc attttattat
     6421 ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa
     6481 gagctaaagc aataataccg tatacataat ctttcgtttg tgtccattca tggaagaaaa
     6541 cggctcccgc aatcgtattc aaaattaatt gaaaaccagt agacaatggt agccctacag
     6601 aaacacccat gtacttcatt ccatggaact gtccattttg tccaacactc caagctaagc
     6661 cggataaaaa tccgatcaag aaaatccaag gggtgattac aggactaaca acgaaaaaga
     6721 caactaaaga aaacagaaat gcaccgatcg tcattcctag tgtttgttga ttggcatcgc
     6781 caccaatttt accacttact aaaccaatac taccccatgc caacataggg actaatgcta
     6841 ttaaaatttc cataaccaat ctccttttat ttcacatgat aagaaactta tcagcgaaaa
     6901 cgttaacaac acaagcactt tttttcgttt ctaacgattt tctaacgttt ttcattataa
     6961 aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt tgattcagcc
     7021 gtcaaatgga tacctaattt ttcggccaga ttttttgcta aataaagtcc taatcctgtt
     7081 gaatgtgttt tgctaagtcg accatttctc ccagtaaatc ccttatcaaa gatccgtttc
     7141 aagtcttccg ctgggatacc gatccctgaa tctttaactg aaaggctgac tccttgcgct
     7201 tccttttcta tgctcacaat gatgtttccg tgatccggcg tatatttgat ggcattacta
     7261 agcaattggt tgaagataaa agcgatccat ttagcatcgg tcaaaacagt atgatcctca
     7321 ccaacgatct cgtagcgaat atttttttgg ataaaataat ttgcctgact gcgtatcaca
     7381 ggctgaatga ttccttttag tccatattct tggatgaggt agtcccgcga gaaactgtcg
     7441 agtcttgcat aatacaaaac ttgttctacg tattcatcga tttttgataa ttcattttct
     7501 agtaatgtat acttttgatc gtcaatatcg aactcgatcg aacgaactag caatttagaa
     7561 gcggaaagtg gcactttgat ttcatggacc catgagtcaa tataatcttt ttggtcttgt
     7621 tgattactga ttgctgattg cattacttct tggtgttcac ggatcagctg attgatatat
     7681 tcttctacca gtttttcttc ttctgtcctc ccaccactca aataattttg caaaggggaa
     7741 gttttttgta tgtcggctaa ttttccccac catcttctct tgctcaagta accacccagc
     7801 agataaaaaa tcagaaagac gccctcaatc aaagctaaat aagcaattgt gctcaaatca
     7861 actttgatgt ttggcgccag ccacatcaca aagatcgtca atgcaataaa aaacagccat
     7921 cccaagatca atgtccactg atctttcaaa tatttaaaaa aattcatctt aaccgctcat
     7981 cctttcaggg aacgatatat ccctgtccta cttttgtttc gatatatcct tggatacctg
     8041 cctgttcgat ttttcggcgt aaccgattga tgttgactgt caatgtattg tcatctacaa
     8101 atcgttcgtc atcccacagt gctcgcagca atttttcccg acttaagatc tttccattgt
     8161 gcttcatcaa aataaataat agacgatatt catttttgct caaatcaatg atctctccgt
     8221 tgatttccat acttccgttg tcaacattca acgtaatgcc attgtggctc atcatttcac
     8281 tagataattc tgtgtagttg taagaacggc gaagtaatgc attgattttt gcaatcagta
     8341 catcgatttg gaaatgtttt gtcacaaaat catctgcccc catgttcata gccatgatca
     8401 tatccatatt cgtattcctg ctggagataa aaatgatcgg tactttcgat acttcacgta
     8461 ttttttgaca ccaataatag ccatccaaaa cgggaagatt aatatctagt aatacaagct
     8521 gtggttcttc tctttcaaag tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt
     8581 caaattgcca tttctcaagt tcctccatga tcaattgtcg aatgatctct tcgtcttcga
     8641 ctaccatgat ttttgccata aaaaatccct ccatctttct tcttgctact acaatatcaa
     8701 agaagccctt gtgaaacaag ttggcttacc tggaaaatgt cattttagca aacgttaaaa
     8761 aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc aagcagctta aatttattat
     8821 gttattctta ataattggtc tattatctgt tatttttctt tcgttgtttt tgtcttttaa
     8881 attttcgata acaccaataa ccgaaaagcg ttagcactag caatatacct agaaggagta
     8941 taagtaatcg ataaagatgg tatttttttg tctgttctat ctttgaagag acttcttctg
     9001 taaagggaac tcgtcttcct gtcactagta atcggtgagt gttcacggtg taaggtgtac
     9061 aagtgataag tgtgactaga tcctgctttt ctctaattgc taatgtattt gtttttgttg
     9121 gaaggacaac aattttctca acgacttcat aagcgagcat ccgaccatac acttcaagat
     9181 agaatcggtc atcttgctcc atttcttcaa gatccgtaaa gagttttttc tcagcaatac
     9241 cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt
     9301 gcaggactgt cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc
     9361 taacattgat tttcggaata tagatggctc cgatcatatg ttgttcgtaa tattcttttc
     9421 ctggattttg gacatttcta accgcttgct caaaaggatc tttgacttgt cccattcctg
     9481 ggattactga tgtatgagca agcgtctgat ttttcttttc atgcactgct aattgttgct
     9541 catgtttttc ttctttatcg cgttcaagct gcttttgata attctcgatc cttctttgat
     9601 ccaaataatt gctgagagca tctgctaaaa atggatatgt aaaaactgca gctccggcaa
     9661 gaaacatcac tgccataaat aattgtaata aaagactgat ttgggtttga ggtctattac
     9721 ctattttctt tttcattttc ttcaccttct tatttaaaaa aactgcagca acctttataa
     9781 aaccgattgc tgcagcttat ttttgttgct tatacttcag acttcatttt agatttacgg
     9841 taccagctgt acgctccaat catcaaactt attccgatta gtaagaatgc caagagcccg
     9901 ttcccaccag tagcaggaag tgacccgcgt ttcatattcg gtactctttc tggccctgga
     9961 ttttctactc ctacgatctg aatttcttca ttatagcttc cataagagat gataaattca
    10021 gtaaaggctg cctcacctgt tggaagtaca taaccttctg gcgccatggt ctctttcatt
    10081 tgataggttc cataatctaa cccttgaacc tcaaagtatc catttccatc tgcgacaaat
    10141 tctgttgctt gtccgcttgt tgcccactca attctatctg ctcctgattc ataaacagct
    10201 aaaggcgact cccctttata aaatattgcg tattcagaac ggtcttcatt ccacaattcg
    10261 aatcttgcat ctttaagcaa ttcactgctt gatgcatcat atttttcaaa ctttcttcca
    10321 cctgttgtga ctttctctgg cggctctact ccttcttcat aatcaaattc atcatttcgt
    10381 ccgatttcaa taaccgctgt gttgttatgg aaatcatccg cttctagttc tgtggaaacc
    10441 ttcaaattat acgtgatcgt taaatctcct ccacgtgcat ctgctaatct acctaaagta
    10501 tctactttgg ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact
    10561 acttctacta tataatcttg tccttttgtt aaaatcgtct cgccggctcg cacttctatt
    10621 tcctgatcat aataagttag accaactgta ggcgtatctg tgattttgta atttttgata
    10681 acagcgcttc cgttttcaag gacagaatcg atgaaataag gaataggtgc tgtgatcgtg
    10741 taagaagccg tttttcctct ttctagatca atataagaaa tcgtttcttg ttctccagct
    10801 tcattaataa tcgttacttg tctaaggtcg ctttcctcta cattcatctg tttatctgct
    10861 tctctgattt cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc tttccatgta
    10921 gaaccatccc acacgccatc caccatatca ggcatcatca ctggcatcat aacaaccatt
    10981 ggagacgcga ttctggaaat atgagctggc gaataagttt ctaagaaaag atagacttta
    11041 tctctagaag aaggtgcttc ttccgtcact tggacggtag agaataccgc ttctccagct
    11101 tcattcgttg tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca actttctgtg
    11161 tgccgttttt gaatccattc aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt
    11221 gttaaaggat tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa
    11281 tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt
    11341 tcttccggaa cagatgagaa accttttttg tgtaatgtaa tcgtcacttc acttggtttt
    11401 tgtgcgactt cttcggcttt gactagtttt ccgttcccga aaatccctat tcccgcagta
    11461 acgagtaaca gtacactcat tcccgtgaca atcatttttt tattcatttt tttctccccg
    11521 ttcttttttt attaataaaa cagcacctaa caataaaaga attcctagta aagaaatcaa
    11581 cgttcttgct tcattcgttt gcgggaatac ggaccatttt cctgattgac ctggattgct
    11641 tggaccactc ggctctttcg gtttgtccgt tgtttctttt acggtctcgt tataaacacg
    11701 tggtgttgcg gattcatagg cagagactgg aactttcccg gattctaatt cagccatctc
    11761 ctgtccgtta actactacat attgtggttg gccttttgca tccacccaaa attcagggat
    11821 gatgacctcg attctcttac ttgcttcatc catctcgtaa ccttccacac cttttaattc
    11881 ttcaaaataa taggtgccag aaggcaagaa acgttgtcct gtcgtaacaa ggccctcttt
    11941 atccgaaatg aatttgctta catttttgtc attcaaagga tcattgtctg ctggtttgat
    12001 ccattgattt ttcaagtcat tagcaggagc catatctaaa taatatttcc ccccttctat
    12061 catctgatat agagcaaaaa cagcaccttc taatggtttt cctttttcag ttgttcctct
    12121 ttgacgtcca tacttgaaga agtatggatc tcttagataa ccgacatttt taggatagat
    12181 atgaatctct tgcagttcct cggttgattc tgtcgggtga taaattggca aaatagcagc
    12241 aattgggata gctgtctgat cgatatcaac atccaatcca acttctggat caatcgattc
    12301 ttcgaaaatc aaataaactg catcccgatc ttggtttttc ttaggtgctt tgatacgtgc
    12361 aatcccgtct cctaatccgc caaccgtatc tttcccagtt gttaccgaag tccctatttg
    12421 gacaagcctt tcttctgtga ttagctgacg gatattgttt ctgttcgttt cagcgatttg
    12481 ctggacgaat tctttaggag tcatgttttc agcagctttt ttttcatgat aataatcggt
    12541 catgtcgtaa atggcaaatg tcgctccatt tagtggcacc gtctgtgaaa tgatatctgc
    12601 tgtttcccct gcttcctcag agatagccaa gccatcattt tcatggtatt caaattgatc
    12661 gttataatcg atatctcgaa tcatccgttt atgcaacacg aattccacga catcttcccc
    12721 tgattcttct gccgacatcg catgtggaag aaggactgct gcaagcggta gtaaaacaag
    12781 taattgaagc atcaaacaca acagcttttt cattaccttg ctccctcctt tttagattgt
    12841 ctgacaaaca aaccataggc tgctcctgca agcgccatga agaatgcagc aagcaggata
    12901 tagaataatc gtccggatcc tcctgtagca ggcaacggat tttcaggatc gttcccgaca
    12961 ctaagctgta atctgattcg atttgtctcc aaaagttcat attctaactc ttgctgtaga
    13021 tcaggcgtct ctgcttcttc gtttggataa tacgaaatct caacttttag tgtacccgct
    13081 tcttttcctg gaactacccg atcattttct atgtcaggtt cttctcgata actaatttct
    13141 aaaataaagt aacctggaag ttttctatag ctgtcaggtg acttcacttc cttgatccga
    13201 tagacccctt ccaacaagtc tgaagcattg aatagggaag aatcgtttgg atctggtgcc
    13261 aagcctccct cgatgatttc atactctcct gcactatttt gtttctcaat tgaaaattct
    13321 gcgccttcaa gacgtcggct attcatactg tcaattttca aaagttccaa atcgattggc
    13381 gctagttcat cttctaaggt tacttgtagt acaccatcga tgatttcata gccttccggc
    13441 aagggagcag tttcgtcttc gatgaccgta tcttcaatga taaacttacc ttcttcattc
    13501 agtgcaaact ggaacacttg atcagatagc cgatacccat ttggtgcgac agattccgag
    13561 acctcatatt caccaataga cagatgcaga ggttggtcgt aatttaaatc gacatacttt
    13621 tctactccta cggcacgaga atagaagtat ccgatcccag gcaaacgttt cgattcttct
    13681 ggttctaacg gattaccagt gtatgctcga tacagcggga aggaagaatc ccccactttc
    13741 ctgatatcga aacctgcacc atctaatgta tggccttcat ggtctaattt tctaactcgg
    13801 atctcccgat aatcaaacgt attgtaagca gtgaatgcta cccataaacg atcctcttca
    13861 tctgttgtat acgtgagata ttgccaattt tcttcacctg tttgcttgag ttggatagtc
    13921 cctatagcat tatcgtaaag tcgcaagatc catgtgtcat tttcatcagg ttctttgaaa
    13981 ccttctgggt tctggctctc aatgattttg tattctgcat agttggtagt ttcttcacta
    14041 tccttataaa caaatgaatc tggctctccg attggatgaa taactttctc accatccacg
    14101 attacatatt tcccgtcttc atctactgaa tagaacgtga gtctcccttt ctcttcctct
    14161 acttcgttca caccagaaag ccctttcgct actggatatc cgatttccag tccgttacca
    14221 tctgtttcat aaagttcaaa ctctgcgcca ttgattggca tattactgcc ttcaattagt
    14281 ttattgatat tcatgtggat tggcttgaat tcatttcgca tttccagttt gacatacaac
    14341 tcatcatact ctgtgccttc tatgttaggc tcttgtacaa cagaaagctt gtgatattct
    14401 ccttcataat aggaattcgg atctttacct tccggtccgc cgactctcga accagatgta
    14461 tattcatcta caagctcgta gtgacctaac atctcatctt cgttggctgc atattttttg
    14521 atttggaatt gatacgtctc atctactaac tgataacgtg gtggtccagc gatctctctg
    14581 atccaaaata tgtcgctgac tgctccagtt tcatctccta aaatcggcat cttcgtttca
    14641 aagatcgcta ttccgtccgt ggtagtttta cttgtttcga ttggttcggc ttccccactc
    14701 tctggatttt ggtcatataa tgcaaattcg actccagcca acggctcgcc attttgaccc
    14761 ttcttgctga cttggaaatt acggccttta tgttcgtaac gaatggaaga agatgggaaa
    14821 tacatccgtc cagtagtgat atcattctca taatcctcca ggttctgaat ataggccata
    14881 ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat caagttttac ttggtacttg
    14941 atcctgacca tttccccttc acctaaagaa atcggagaaa atttataagc ttggttttcc
    15001 cgtccgacat acgttgtcgg agtcgccact acttctggtg gggcttgtcc atgacgttct
    15061 tcatcccata tttctggctc ttggtacttc atgccgtttt ctcctggatc tagatggtaa
    15121 aactgaatat ctgccgatct gccagctcca aatggtgaga cattaaaacc agtttcatca
    15181 taaagtgtgg cttgctggat cgttctcttg aaggtagaag agatatcctc caaggctgta
    15241 acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg ttacgccatc ttcttctaaa
    15301 tcagcaatat ttttcaatac atttcgtcca atagcatcta gtcgttgtct cccgctaacg
    15361 tttccatcga ttcctaaacc aatggagaag atacttggcg ggttctcttg atacttgttt
    15421 ttcagccaat gatgataagc aactgtgttg atcgcagatg acttttcttg cgaagtagga
    15481 ctgccattcg tattgttatt agaagaacca tcaccaaccc agtgagtcgc tttattatct
    15541 ccttctttta cctctccata acgatagtgt acatttactt catcttctgg acggttaaag
    15601 tcagatgcat aggtaactgg atgagtaaac cctgcattag aagtattccc agaaaactcc
    15661 tcaaaatttc tgaacaaaac gtcattatct tcagcaatct tatcttccat cacagtccag
    15721 ttgctccaag ctccattagt gctcgtacga taacttgact gaatcggtgc ataactgaag
    15781 gtcggtgtcc catctcccac aacgattagg attttttctg gatttctatt ttctccgcca
    15841 ttgtctttat acaatgtttc atagccattt ttaagcccta gtgtaagcgg agttcctccg
    15901 attggtgtac gatttaatgc tgtattctca cttcccgtca aacgattgat atcatttgtc
    15961 aatggaaaga tgtccgaact gatttgctca tggttattga tagctgtact attatggtaa
    16021 aaattcacca taccgattcg aatatccaga ctcgggtcag agagcagatc tttactcatt
    16081 tcgtttactg cctcaatcaa cgcagcattt ttagtttgac tattggtccc agcggttaat
    16141 tcactcatac tcgctgactt atctagaaca aaaacgatat cgattggact gatttccttt
    16201 ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg ttgttgcatc ttcaggatgt
    16261 agatcagatg ctgtgctgct tggcagaaca ggttctaccc attttttgat gtacgccccg
    16321 ttataatcca agtaactgtt ttcaaaattc cgttcatttc cccaaagctg aacggtttga
    16381 gctggaacgt ttctatcttc ttcgcctgca gttgatccat tattagtagc atagtcgtaa
    16441 tttcgccagt tcgctccccc gtttgtataa tcactatcat cgatcaagtt ctctccgtca
    16501 tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt gtgtccaatt tcctaacact
    16561 tttgtatagt cattcgttgt aaacttcggg tagcgatgct gttcgttttc aacttctccg
    16621 ctaggataga tatattcata ggggtcttgc gtagctgttg gcccaagacg ctccaaaata
    16681 ccgttgaata actggcgttg aatgcgctgg agtgcttgac cgctgggtac gattggttcg
    16741 tccagctttt ccatctcatt ttcttcgcta cgttgaattt cagcggaatc ttctgtatca
    16801 gtttcatttt cttgtaattc ttggttatct attctttcat caaccaccac ttgaagaggt
    16861 agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt gccggtttct
    16921 gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt ctcgactttt
    16981 aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt ttttacggta
    17041 ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt ggcactttcc
    17101 tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata cttcccttca
    17161 atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt tgactcagca
    17221 gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa tagcaaactg
    17281 acagttacta actgcaaaag cttcaatttt aaccctttca catccttata ctcctttcaa
    17341 aaatatttgt cttctttcac aacaatcaac aaaacaatgc ccatctaatt ataatttaat
    17401 ttaattttaa ttttaaatca aatgatatcc tttttagtta aaaaacacca attatacaac
    17461 atttattatt aaaattaaat tataaaattt tataacgttc tcatttaaca aaacttaagt
    17521 tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata aagcacaaat
    17581 gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc agcgcatagg
    17641 atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata atgtaaaagt
    17701 aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat tcctgcaact
    17761 ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg tcctgaagga
    17821 aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt ttcataaggt
    17881 cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata gatcaaactg
    17941 ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa aacagcagca
    18001 aggagcaacc aaataaaacc accattagag atattactga taaatttcaa aaaattgatt
    18061 tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc atcgatttta
    18121 gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg tacgatggat
    18181 aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa aatagattgg
    18241 tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga aaagcgcgct
    18301 tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt tttgttccag
    18361 ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa acaacgtagt
    18421 atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat aatcgatcaa
    18481 tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag agaagttctc
    18541 acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt tgtaatgata
    18601 aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca agacgcacag
    18661 gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt ctaagaaata
    18721 atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc tgtcaaatcc
    18781 atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg gatcaagaca
    18841 agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg aaaaacttgc
    18901 aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg acctaagaaa
    18961 tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg tttcgtcaat
    19021 ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc agcagcttcg
    19081 atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt gaacgcattg
    19141 ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat cttggagaat
    19201 aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga aagaaccaaa
    19261 gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc aataaaagaa
    19321 tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt tcttgcatag
    19381 cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat tttttctggg
    19441 aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt aaacaccatc
    19501 caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata acttagtatt
    19561 tttttgtttg ccgattcaca ctggtctttt tgaaataatc catcatttgc cgtcacctcc
    19621 agaataatag acccacttag gtgcaagctt caaattgaca aacgtcagaa ccaacacgat
    19681 cagaaacagg atcattgaca tagccatcgc ataacccata tcaaaagcta cgaacgcctt
    19741 gttccacata tgcagattat aaaaaagcaa agaatttgca ggtccgcctg ttccattttt
    19801 cgtcatgaca taagcttctt gaaaaatttg gaaagaaccg atcgtagagg tgatcacatc
    19861 gtaaaaaata ataggagtga ttaatggcaa agtaatactt ttgaactttc cccaaacgcc
    19921 tgctccatcg atttctgctg attcatataa agatctagga acattttgta atgtagctaa
    19981 gtaaagtagc attgctcctc cagaactcca tattttcatc aaaatcaacg caggtttcgt
    20041 ccattgtgga tcaaacagcc aagctggtcc agggatatgg aaccagccca gaaatgtgtt
    20101 gatcaatcct gcagatggag aaagcagctg catccataag atgtagactg ctactcctga
    20161 taagactgcc ggtaaataga aagctgtacg gaatacactg attcctttga ttttttgatt
    20221 gagtactgtg gctaaaaaca cactgaaaat cgtcgtcagc ggtacattga agatgacgaa
    20281 ataaacggtg ttgtataacg atttccaaaa taaatcatcg tgaacgaaca ttcttttgaa
    20341 atttgctaga ccaatgaaat tcatcttgga tgtcaaatta taatcggtga aactaccaat
    20401 aactgaaaaa agcataggcc ctagcataaa taccaagaac ccgataataa aaggcgagat
    20461 aaataaatag gcccatcttg cttctttttt ctttcggata gataaacgct tcaaattttc
    20521 ttgcctcctc tgtacctaga cagtcaaaaa tggattattg attttgcttg actaagtttt
    20581 ctactgcttt ttgtgcatct gccaatcctt cttttggtga ttttgttcct aaaagaatct
    20641 ggtcaatctg ttcattcact aatgtcgtgt aatccggagc atctaaagga actggtgtga
    20701 tcaccgtatt tttaaagttt tcgtccgcca tttgatagat catttttcct ttatccgtta
    20761 ggttgtcatt attcactaaa ttctcattgg ctgtcttgct cgccatgata tcaaaacttt
    20821 tttcgccgaa tttttcttgt acttctggag aagaaagata tttgatgaat tcataagagg
    20881 cctcaggatc ttttgctccg tatggtactt ccaagacgaa tccgccaccc cacgaccaat
    20941 ggcctgaacc ttcttctttt tctgggagct gcatcacgcc aaaatcaaaa tcatccggtg
    21001 cattttccat caaactagag taataattga tattttgggc acgcatggct actaatccgg
    21061 aaataaatgg atcagataca ccagacccaa attcggcttc taatttattg atcgtatttt
    21121 tcccataata atcttgccag tcttggatcc actccaaggc ttccacttta tttttagtat
    21181 caatcttgac tttttcatct ttgtcaaacc aactagtccc gttatctgcg tttagcgccc
    21241 atacatcagc ccctaaattc cataatggat aaaaaccgat gcgtgtaaat tcttttcctt
    21301 cttttttatc tagtttacgt gctgtttctt ctaattcatc ccaagttgtc ggtagatctt
    21361 gttcactgat tccagcttct ttaaatatct ttttattata aaaaatgact tgtgtatctg
    21421 tattgaatgg gatcgcataa ggatcacctt catattctac tgtatcccaa agctgcggat
    21481 aaaaatcctt agaagtgtct ttttctacat atttggagag attagttgct tgctgtgctt
    21541 ctgctctttg ggcaacggaa tttatgtcct gtacaataac atcgggtgca tttccagctg
    21601 ttattgcaga taaagattta gtccagatgt caccccatgg ctgatactta taatttacat
    21661 ggatctcgtt ttgtgattga ttgaaatcct caatgatttc ttcgatagct tcttttcgtg
    21721 caccggatcc ccaaaaggac cagaaatcaa tattttttct tccatcactt gttttttcag
    21781 aagaatttgc tgcacttgaa ctggcatcgc ttgcattact tgatccgcat cctcctaata
    21841 aaaacaatgc tgaacttgcc agcaaccctg taaacaataa ttttttcata ttttacctcc
    21901 aaattttaat gtaaccgatg ccggttttaa tataacgata tcattttgtt ctgaaaataa
    21961 caatttataa ggtatgattc taaaaaaatt attaatgata ccaatacttt atataattcg
    22021 cgagcgcttt ctttgaattt tttaatcttt cgtagcagaa tgagaatgta ttaaaaatag
    22081 attaggagga attttgatgt ttagacacca gaaagaattg cagtttgaag caaaaccaga
    22141 tcgtccgaat ccattaatcg cgaaatatct gcaggagctg atcggcggcc aatatggcga
    22201 gatgagtgta gctatgcagt acctgttcca aggctggagc tgccgaggag aagaaaaata
    22261 taaagatatg ctgatggaca ttgcaacaga agaaatcggc cacgtggaaa tgttggcgac
    22321 aatggttgct cgtttattag agggtgcgcc tgttgaagat caagaagcag cgatgcaaaa
    22381 agatccaaca atcggtgcca ttatctctgg aatgaatcca cagcacgcga tcgtagctgg
    22441 cttaggacct cgtcctgctg acagcgtggg caatccatgg agcggtgcct atatcattgc
    22501 gagtggcaac ttgttagcag actttagagc taatttaaat gccgaatcac aaggaagact
    22561 tcaagtagca cgtatttacg aaatgatcga tgatcgtggc gtaaaagata tgctgtctgt
    22621 tctccttgca agagatagct atcatcaaaa tctatgggaa tgtgcagtga aagaattaga
    22681 agaaaaagaa ggttcaatct tagtcccaag tactttcccg cgtgacaaag aacgaaaaga
    22741 cattgcttat gacttctata acttctcaga gggagaccaa agtagtgaag gttcttgggc
    22801 tagtggcaaa gcactggatg gcgaaggcga atatcaatgg ttgaaagaac ctaagatcga
    22861 aggcgctgca ccaaaattag aaccagtaac accaaaaatg tatgggacac ctccattcaa
    22921 gaaataaata tctacccgta gaaacacccg ttttttgttc cacacgcttt gtgagcaaaa
    22981 ctgactaaag tcattaatca taaccaccct tcaaaagggt ggttttctct agagctataa
    23041 gctcaaatca ccggccagcg cctaaagacg ctggctttca tattttatgt tcaagccata
    23101 ttgcctttgc cagttttgcc cacctcttaa aaggtttatg tactactcct tagcccttaa
    23161 aaggatcgtc ttactctcgt acactcagct tatctaacgc actatcatgt ttttcttgtt
    23221 cttgaatgta ctttttgatg gtcgcttcat tttgtcctac ttttgtcaca tagtatcctt
    23281 ccacccaaaa gtgtcgatac ccaaatttat attttagatt tgcacaatct cttgatgatc
    23341 tcctgaatat tggctcgata ttgattataa attatttttc ttctatactt tggtgtgaat
    23401 acaatgtggt atttacacat ctactttgta cgggctaaac tatttccctt tattgctcca
    23461 cccgcatagc aagtggtttt gtatttcaat cgctcggcgc gctcaattag gtctaaagac
    23521 aataaatcta aaaacccgct gcttcactga cagaaacaac gggttttcag aaaaaaatga
    23581 aatgaaaaag atctcttacg gcaatctatt ttattttttt gattttttat aaatggaaaa
    23641 tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa gaacctatga aatctttatt
    23701 tattagatat tcaacagaaa tcccgataac actagccaac aatatattta tcagaagtgt
    23761 aaaataagga tgctttttat agaatagaac tagtgttgtt ttcatagact ctcctttctt
    23821 catatttcca attaatcaca aattcacggc attactagaa aaattaaagg ggcgcagaag
    23881 tggaaaaata ctcttcgact ttccctacaa accaggccat cttgtgttta ataactatac
    23941 caaatctaag gtacctctaa atctaaattc catttgtggc ggtgacctcc aatttttaac
    24001 tcttctatct cccatccagt atacataacc ttgatacttc gaccacttat atatcgaaca
    24061 cacaaatacc tatccattgc aggagtacta cctaacttag ttgctacttg catctaaata
    24121 acagctgaaa agataggact tactcttagt aaagtatctg aactattaat ttttaaacta
    24181 tcatttttat cctcaactac atgcacatct gttgcaactt cactttctgt ctcatccaga
    24241 taaatgatta aggaatcggg aattgcaata tcttcagctg acgctggaat agttgagcta
    24301 aattagtcat catatcttta ttataaaata accaacagca aaaaatacaa tgaaaaacaa
    24361 acttggaact aatgtatcat taataaatgt tgaaaaattc agcaactcat tactgtatga
    24421 ttttagcagc ccaaccaaca tataccctat aaaagctaat aagatggtta ggaatccttt
    24481 agaattttta ctcattcata gacacttcct taaataactt ttattataga aggtattgta
    24541 aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa ccttacatta tactggaatg
    24601 aactgtattg atagtgtacc taaaagctct actgacataa gatcgtgtaa tcggtaaagt
    24661 aattttatta attatacggg atgtaatcaa ttagagaata aactaatcat aaaaggtact
    24721 tttacattca taattgtcgc tttgagattc tactttggtt cactatctga cccaattgca
    24781 cattttcgat cattacaagt attggtgtcg ttagaacaac taaataaaaa ctagcggtag
    24841 atttttcaga atctatgcta atttctgtat ggatgttcca gtgatggcaa atttctcttt
    24901 tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat
    24961 tctttcctag acagatcttg atcccaaatt ttacgttttt gagcatcgat atagtttaaa
    25021 atgacattac tcatatctaa agagtttaga ttttttagga ctaaaactgt cagttgttct
    25081 ttttcttctt tctgttaaag tattgtcgcg acttttcgta tacgttaaga tatgtcgtga
    25141 aaaaacaagt tttgtgacac aaaaaaacga atgacttgag gaaatcacct caatcattcg
    25201 tttaaaatgc tatatattct ttagtttaag atacggaaat taatgccatc gaccacacta
    25261 gtagaatgaa cgacatttac ttggcctttt tttaatttcg ttatgtttag catataaaaa
    25321 tattatagag tttacttaca atgcttaaaa catatactgt ataatctgat caattttagc
    25381 catgtcttgc aatggtaagt tttctatttt tttgagtttt cgttccttaa aatcaagaga
    25441 ttttaattgt gtaataatta cttgtccttt tatatctagc tcttcaggta atgagtaacg
    25501 tgtcggtaaa ttctttatag tggaagtaat tggacatatg accgaaaaca ttgttttacg
    25561 gttaaattca taacgagaga cgactagtcc tggtcgtctt ttttgaattt cttttcctac
    25621 ggagggatca aaatcaatcc atacaatatc acctttttta ggaatataaa ttctttcttc
    25681 tgacactata aaatttctct cccttcagga gttaaatctt cccattcgtc tttctcataa
    25741 tactcggcct cttccccacc actaaatgga tcttcaattt ttggaactaa cgtaattgtt
    25801 ccatcatcag aatacacaac gatatattct tggttttctg aaggtttctt tccgttatca
    25861 gaaggtaaag ttaccactac agaacttcct tgtaatctag attttgtaac aagcattatc
    25921 atcactccct taataaaagt atactatata acactaagta acgcaaaatt cccctttgta
    25981 aactatctag aaaattgttt tgtttaaagt atatcttatt ggatataaat agaaataaat
    26041 tgtagccagt tcattttttc tttaactaaa aaaataatcc aagaaacatt gaacctatgg
    26101 atccaaaagc tacccaaata ctatagctaa ccccccgcta ccttcaattt tgcagataaa
    26161 acgctttggg agaaaatttc aaagacaaca ggccatccta tcatgcccga ttataaacaa
    26221 aaagctggct attccttttg ctctgacaaa tttcaattgg gaaagttcac gaaactagca
    26281 ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga aacattggtt
    26341 caacgaattt caactagaca agactttgct attgtagaac gtgagatcgt agctattttt
    26401 gtagaagaag ggattttaca tgatccaact catatcaatg tggataaatg gcaacctctt
    26461 atctataaat tcagagaata tacaactgcc aaccagtcgt taactttgaa ttttagattc
    26521 caagaattca aagagtcata atgataaaca tcgttaggta taaataaaaa agactcgcag
    26581 cacgagtaaa aaacaaatag aagctataca ttagaaaggt cgactgtgac cctatagatg
    26641 cagtatatgc agcttatgag catttcttct gttctctttg tttttgggat ctgtgctgaa
    26701 gtctttttcg ttttttgccg ctctttcttt gaaaaatcga ttatgttata ccttctccat
    26761 tactaattca ggcatctgct gagtaatgat ttttcgcgca aaagaaatat ctgttaccca
    26821 aggttcgatt tgttcttcag tcaagacaag cggcattcgg tcatggattg gtgcgactgt
    26881 gcggttaggt tcagtagtca ttatgattga ttctgccatt ttttcatcct gatggctacg
    26941 ataaaaaccg ccaatataca ctattttttc cttatttgaa aacataaatt tgtctttttc
    27001 ttcattccac tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt
    27061 ttttcttcta ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta
    27121 aatccccact tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca
    27181 acaagatttg acggaaaaac ttctccagct gctaattctg ctgcctgctt cggttgtttc
    27241 gtctgggctt ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga
    27301 ccacacatcc taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc
    27361 accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct
    27421 ccagtgaatg gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
    27481 gagaagctgc tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc
    27541 tctgggtaaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
    27601 gtagaaagct attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa
    27661 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
    27721 aaatatgagc agctattttt atttggctgt tcttattact tgatgcaggg cagctttttc
    27781 acaacctctc cactaaacgg tgttcaatca gctaaccttc ggtattagct caaaaaacgg
    27841 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt
    27901 tcacaacctc taatacacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt
    27961 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    28021 ctgtcacaac ctctaattat taaagaacct tttgcgaagt taacaatctt tctttgatca
    28081 attgaaaatt ctgcctaagt agtgatagat cctgtgtgac tttcaacaat aagatacttc
    28141 cctggatttc agcaatcaac gctttggctt gacggtctgc ttcttcatcg gacagttttt
    28201 cttcagaaag tgccaggata ttttgtttcg tatacttgac ccattgattg actaattcta
    28261 gtagcaacag acggtattct tcatccactg tactcaactc aataatcaaa ttacctacag
    28321 gacagccatg ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc aacatttctt
    28381 ctaatgcttc ttctggctgt tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc
    28441 atacgtccat atgcgcatgg atcaccgcta gacaaacttc tttttttgaa gaaaagtaat
    28501 aataaaattg accttttcct gtatcagaag ctgctagaat atctcggatt gacgtttcat
    28561 gatagccttt tttataaata agatctttgg tcgcttccaa gatcttttcc tttgttttca
    28621 atgatattct cctttttcat ttctcatagt cctatattat acagtaaatt catcattttt
    28681 tcttcttaat cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa
    28741 ctgttctcat atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct
    28801 atcttatgat agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc
    28861 aaagttcgtc aacgctgcat ccattgcttc ttggccagga gcaccaattg catctgtcgc
    28921 taccacaaca ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt
    28981 tccgttcatt ccaccaagaa ttactgtgtc gattccattt tttctcaatt ggagcgccaa
    29041 atcattgctt tctggtccaa agattttatg aggagagctg atgatcgtat tttcatccaa
    29101 ataaggtttc aattcttcga taaaatcggc tcctgaacct tcttccactg attcatattg
    29161 actttttcta gcaaacatct gctgctcaag catcatatgt tctcctgcac cgccaaattg
    29221 ccatttatgg tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc
    29281 ttttgcttgt tttaccattt caatcatatt ttcgatcgta ttgtattttt ctaagatatc
    29341 ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag
    29401 aactactgct gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa
    29461 tacatcattt agagcttcac tcattgtcgg atgagaatag atttgatctc tcaataacgt
    29521 ataaggaagt tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc
    29581 cgcatagata cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac
    29641 acctttagga tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa
    29701 tttgtaattg acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt
    29761 cggagtgata aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc
    29821 gcccttcaac tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa
    29881 tccgcctttg acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc
    29941 cacagcgatt gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc
    30001 tgtacttggt tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg
    30061 gccatcttct gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga
    30121 tacacctaga tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat
    30181 atcttcatct tcacgtggaa gaaattcaga atgggcatca agaacgacaa ctttagatcc
    30241 gtattcattg aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa
    30301 tttttccggt aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt
    30361 attcaatcct ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc
    30421 gccttcaaca atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa
    30481 acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt
    30541 ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc
    30601 tctttctcca ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc
    30661 gccatacata cgtgttgact tttcaacgac taaaaccgat tcaccttttg aagctaacgc
    30721 cttcgctaaa gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat
    30781 catttttccc tcgcttcttt tttacctacc gattggtaca aaaatatgat aaccgataat
    30841 tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa aagcggtcct
    30901 ttcacgtttt tagtctggtt cgatttcctt tattcttcta aacaatcgtt acttctacat
    30961 cgatatttcc tctagttgct ttggagtaag ggcagaattc atgcgcatct tctgcaattt
    31021 tttgggcttc ttcttcgctg atgccatcga tatgcacttc taatactaca ccgatctgat
    31081 agccgccatc atctaaatta taaagagaga cacgtgcttt tactgtggac tctgctgtca
    31141 ttcctgcttc tttgatcata tgttgtaccg ccccattgaa acaagaagca taacctgctg
    31201 caaataattg ttctggattt gttccttctg catggattcc tggaggagtg attttcatat
    31261 gcatcgagcc atttggtgcc tcgacttctc cattgcgccc gccatggttg atcattgctg
    31321 tttcgtatgc ttttttcatt gtactgctcc tttcgattga ccatttgcta cttggctata
    31381 attatagtgt atgacaagta aggcgctttc tacaaaaatt ctgcttattc gaataccaga
    31441 atgccgtctt ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc
    31501 atatttgttc caaaataaat agcaatcttt tctacataat cttttatatg attgatttta
    31561 agatacatat ataccagaaa gttgacccca taaaccaata ctgttaaaaa aataaccata
    31621 ttcgttacca ttttttcctc cttttgctgg tctccctacc agcactcgat ttaaccatac
    31681 cacttttttt gccgtccaag caactagcct ttcaaaaaga ctggtttatt ctgctgttaa
    31741 tgattcaaga tcagaaaaat gtccgaataa ttttgctaat tttactaacg ccaacttgtt
    31801 ctttccgaat tcatcatttt ctatctgtaa tactagtaac ggctcatgta agcttaaacg
    31861 gatcaaaaac cagccgtttc cgtatggctc ttctagatca tatcgaatac cttcttcatt
    31921 ttccgggtcg aagttcatcc ctttttcttc ttccaacagt cgcttcattt cttctaatat
    31981 cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt aatcggattt cttgtacttc
    32041 tacgggctgt ttcaactctt tgatttgttc atctaatgtg ataccttttc tttttaattc
    32101 tggcaaaagc atcaggatct ttgcgatgac atatgcacca tcatccagaa aatagttttc
    32161 cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt gtatctttcc caagacggtt
    32221 aagttctaat gctttattga ttacatttcg atagccggaa atataacgaa cttggatgcc
    32281 tcctttttct tcaatgaatc tttgtagatg agaagaagta ggggaattcg tcacgattct
    32341 tgttcctgga tgctctttta atacgattgt acttaatact gcgatcaaat tatttcgatt
    32401 gatcactgtt ccagaagcac tgacaactgc agaacgatcc acatctgtat cgaaaatgat
    32461 cccaagatct gcttcatttg ctaaaacagc ctgcttgata ctttccatcg cttctttatt
    32521 atcaggattt gggatatgat taggaaatcg gccatctggt tccaaaaatt gggaacctgt
    32581 tgaatccgca cctaagctat tcaatacttt ttcagcaaag aatccaccag caccatttcc
    32641 ggcatcaaca atgattttcc aaccatttag tggtttgctt tctagtgttc caatcccctt
    32701 tttaattttt gtgactaaat cttgcgcata aggtgtcaaa aggtctgctt tttctacgct
    32761 tccagtttgt tcgcttttta ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt
    32821 ttctgctcct ccttctgagg taaagaattt tattccatta taataatagg gaagatggct
    32881 agcagtcagc ataaccgcag catcgcacga gaactgttca aattgagtgg acataaacat
    32941 agcaggcgta gtagctaatt cgaaatcgat gacatgtatc ccttgttctt tcagactttg
    33001 gatcaacgcc tctttcagtg ctggtccgct tatcctactg tcatgaccaa tcccaatcgt
    33061 ccatttttta tttggatcgg ttgatttctt ctttttcttt atccagtttg ctagtcccca
    33121 gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc
    33181 gattcctcga atatccgatc cattttgcag cgctttcata tctttcatat aaaagtcctc
    33241 ctcatttatt ccctcatttt ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag
    33301 cggcaaataa taatgaatgt atctacaccg gccatcgttc cgacaacttc agggtaagcc
    33361 acttcatcta gccaattcgt taccacatcg gcaccatttc gttcagtcaa tacgataacc
    33421 atgaactgaa cagactggat acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct
    33481 tcatttaaag aaacagatgg ctggttaaat aatgtatagc gtactatttt gttttcattt
    33541 tgacttttta ctaacttcat ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg
    33601 gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta
    33661 atgatctggc ggatcagtgc ttgtcgctgt ttctttttca tattagacac ctctcaatgt
    33721 tgattatagc aaaagataag taatatttct tcatattttg gtatttttta cttttatttt
    33781 ttgttttcat tcaggttgct aacctttttc tattttaaca cgcaaaatcg tcttcatttt
    33841 ttcaggtgct gattttcttg gatcgctgat gtaaatctct ttgtgttctt tagaagctct
    33901 ttcatagcct tcttttgcga tgaatgcttc taatttctca aacgttgctg gttcttcggc
    33961 atagggcccg acgtgtaaga tttgggcaca aagaccttct tccatctttt tgaatactac
    34021 ttgttctttc aagtcttccg gaagtttgct agaacgctga atggcttctt gtgctatcgc
    34081 ttctgtaaca aagtcaggct gtcggatcat caattgataa gaaaaatgtt cttttttcat
    34141 cactggttca tgcaaatact tttcttgcag tccccattgt ccttccagtg gatagacagt
    34201 atacatctga taatcaggaa tcagccaatt ttttttaggc gacatgcgta ttgcatagct
    34261 gactgcatat agacaggcta ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc
    34321 tctgatgcta aagaatgatt ttttaggaac ctctagtatt tgcgggcttt ttttcatact
    34381 atagctcttg tctttacgcc attcaatttt ttccattttt cttcctccgc gaaattccat
    34441 ttagttattt attatcatac ctctttttga gtgacttggc taatcgtgtt gacactattt
    34501 ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac agataacacg tttcggcgtg
    34561 tcgagagaga ggcggtttct atttttataa tagaaatcac tctctttttt tacgaaattt
    34621 tttagaggtg atgtaaatga caaaacaaaa acagctgctt ttagtacctg cagtaattgc
    34681 aacagggatg atgtcttttg cgggcgtatt gatcgagacg gccatgaatg taactttccc
    34741 aactcttatc agacagtttg atttaacgac tgcacaagtc caatgggtaa caacgattta
    34801 tcttttgatg atttcgatca ttgttccgct gtctacttat ttgaatcgca attttagtct
    34861 caaaaaactc tttttagctt caaatctgct tttcttggca ggtataatga tcgatttctt
    34921 ctctcctacc ttcatggtcc tattgtttgg tcgattgctc caaggtgcag cgacaggtat
    34981 cgctcttccg ttgatgttcc atattatctt gacatttgcc cccctcaatc gacgaggcgc
    35041 gatgatgggt gtcggtactt tgacaacggc cattgctcca gcaattggcc ccacttatgg
    35101 aggcattatg acttcacatt ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt
    35161 gtttctttct ttgattatcg gctggtttgc tattcccgag atggcagtac aaaaaagtgg
    35221 ttatttggat tggataagcg ttttaggaat ttgtcttttg tttagtggaa gtctaacatt
    35281 tttgagtacg ttgggaacag tttccagctg gattgcttta atcatcggcg gtattggctg
    35341 gttccttttt tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac tgtctattct
    35401 ttcaaataaa acattccgct tgtttttgat tggcttctta gtttatcagt ttttattact
    35461 tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt caaggaagta gtccttttgt
    35521 cgctggatta gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc ctttttccgg
    35581 gaaaatgctg gatcaatatg gtccgaagaa gccaattttt gctgggctga ttctttcttt
    35641 gattggttgg ctagcattat tcctactcat aggacacgct tcagtgggtg tattgattgc
    35701 ttgtcatgtg ttttatatga tcggtatcgg attatcttac agcaatatga tgaccaccgg
    35761 aatgaatgca ctgaatgaag aactacaagg agacgggaac gcaatgttca ataccttgca
    35821 gcagttttct ggcgctgtgg ctacttcatt agtagcggtc attatcaatt acgtccagca
    35881 tcatacttcc cataactatg aagtgtctac tacattagga tcaaaagcag cattaggcgt
    35941 cttgttgctg ttgcttctcg taagctttgc tcgattcgcc tattatctgt tctttgctaa
    36001 aaaagcgtag attccatata cggcaggtgt tgcatctgcc gttttttttg tgtgaaaatg
    36061 gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg gcgggatcat
    36121 tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt tatttgatga
    36181 agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt tgtcgaaacg
    36241 cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc ctaaacttat
    36301 aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt tgcttcgacc
    36361 tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag aagcgccaga
    36421 aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc cgttattgaa
    36481 accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag catatttaga
    36541 acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa aagtcacttt
    36601 gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca cagctgatcg
    36661 tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag gctatgctgc
    36721 agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata agatgagtca
    36781 agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca tggatggaaa
    36841 gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc ctactagcga
    36901 agcttataag ggattgacta agagtacaga acagttcttg actgatccag atcagataaa
    36961 aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt tttccccatt
    37021 tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag gctcttctgg
    37081 cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata ctggtcattg
    37141 gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc catcatagaa
    37201 aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact tcttgaaaaa
    37261 gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg cttcatctgg
    37321 caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt
    37381 caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta taggtaaata
    37441 gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca cgattctttc
    37501 aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc cagtattcag
    37561 gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat agtcttccct
    37621 aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat ctagctgtct
    37681 ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac caaaagacgc
    37741 tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt tcatgatcca
    37801 aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg cataacgtga
    37861 tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt tttctccttt
    37921 acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg ccggatcatc
    37981 agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag caaaaccatt
    38041 ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac cttccgtatg
    38101 ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag ccatcgtata
    38161 ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa agccattgcg
    38221 atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa
    38281 tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat ttggaaatgt
    38341 ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa aggaaacaga
    38401 cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca aataagaata
    38461 caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa attcttcaga
    38521 taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat acacagcttc
    38581 tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga tccgccgagc
    38641 acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa catcagctgg
    38701 taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc ctggactccc
    38761 ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat taccgtaaaa
    38821 tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg tgatttcgtt
    38881 taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca aaaaaacgtt
    38941 ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg aagcaaacga
    39001 gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg tttcatcatt
    39061 ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc agcatcccat
    39121 gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc agctgaatag
    39181 tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc gttagtagct
    39241 gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct tgaagttggc
    39301 gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg tgcaacgtat
    39361 gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt tttgttttct
    39421 gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac ataaatgcgg
    39481 ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt gtctcctgca
    39541 aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac ttcatcagcg
    39601 tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt tgtacctaaa
    39661 acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt tattctttcg
    39721 acgctattaa ctttatcatt tagaaatatt tcataggtaa aagaaacatg acatttaatg
    39781 acaaaataac gaattattta atatttgtaa catttaaaca aatatttttt tgatacaaaa
    39841 ccataaaagc cttgttgtat aagcaaagta atcgtttttc tatgtttttt tatttttgaa
    39901 tgaaaaacgc agattctgta acattcactg caataatcca taaaaaaaag caaaaaaatt
    39961 tttgattttt atcaaattct cttaaaaaaa tgtaaaaaat acaaaaaaac cagtaattgt
    40021 tacaacaatt actggacaaa aactttgaat aatgttaact atttttttat tttaactttc
    40081 tccgcaataa tttctgggca tgttccaatt cgtttataac gacttcttct gtttctttag
    40141 ctaatatttc atctaataca tcaagccatt tttccggttc ttttgttcct agacgtccaa
    40201 tagcccaagc tgctgttcct ctgatgacag ggcggacatc tgttaaacat tctgaaatct
    40261 caggcaatgc cgttcgatca cctagattag ctaaagcaat caatgcgttt ctctgcaaag
    40321 gtttcttccc acgccatgaa ccagctaaat gcccaaattg ctgtttgaag tctttattag
    40381 acatcgaaag cattggcttt aattttggat atatttcgtc tacttttggt tccatctctt
    40441 catgaaaatg aaaatctttt ccttgattgt aagggcagac taactggcag atatcacatc
    40501 cataaatgac atttcgcatt tttttacgat attcttccgg catcatccct tttgtctgag
    40561 tttgataaga caaacatcgc tgggcattca ttcttccgtc accaagcaat gcttttgtcg
    40621 gacaagcagt cacgcagcgc atacagtcac cgcaaccaaa ttctcctggt ttatcaggct
    40681 caaatacaat attagtaacg atctcaccaa gatagacaaa tgaaccgaac tcttcagtga
    40741 tcaacaatcc gtttcgtcca ataaatccta aaccagctcg ctgtgctaca gcgacatcga
    40801 tcagttctcc agtatctact tgcggagcta gacgccattc ctcttctttt tgccattttt
    40861 ctgctcttgt ttggatgaat tcgatcaatc ggttcaaacg gtctctgagt atatcatggt
    40921 aatcgatccc ccaagaagca cgagcaaact gcccgcgttt ttcatcccta ggcacttctt
    40981 catggatcct agtaggatac gcaagtgcaa tggaaatgat cgatttcggc tgatcaaatg
    41041 tcatttctgg ataaagacgc tcatcgatat ttggatgttc aaagccagaa gtatggccag
    41101 ctgctttttg ttcttccaac gattctttta atgaatcaaa aggttccgcc gtggtgaacc
    41161 cgattttgtc aattcctaat cgtttgcttt cttgtatgat ttcttctttt aatgtcatgg
    41221 tcttcctcgc ttgctgttaa cggctgtata acccataata tagtttttct tttttcagct
    41281 ggacaaactg gatctgctga tcggcaatct tcttttcttc catccatttt tttcttgcag
    41341 caaatacttc ccaataattt gttgaaatta attttctttt gttatctaat gcattaaaaa
    41401 aattcaatga aactggtttt gtacttaaca gttcatggag ccttacctct tcccatggcg
    41461 aaaactcaga cagatcgcgg gaatacaata cttcttcata gagatttttt tgctgtgtgt
    41521 acgtgaatgt ttgcgggatt ttcccaggcc tagcaatttc tactagacga ttataaaaca
    41581 tattcattaa tttgctgttg gcaataaatt gacttttata ataggccgac tggatctttt
    41641 tgattactcc ttctgctttg atgactgaaa ataatgcagg ccatgctttg acggttattt
    41701 cgtcgttttg tttaactaat acgccaggtg tgaagccgac tggagaaagg aacacaaaat
    41761 aaccgatacc ggtattttca tctaaaggct gaaattcatc aaacaaccat ttgcctatgt
    41821 acagctcttc atctggttct gttgtctcat tttgatttgc tatgaataat ttagctaatt
    41881 ctgcttcaag aaattgaagg tcttgatcct tgtcggtatt ttcctgctgg ctgatcaatc
    41941 gaaagacttc ttctttaatc gtatatttat ctttacgtgt aaaattcata gccacacctt
    42001 ccttccttac tctaactata acggaatttc ttcaaaaaag gtatctgtac gacgaaaaac
    42061 agcataattt tttttcatat ctcttataaa aaatgcaagg tctgctcctc aagctagacc
    42121 ttgcactttt cagctttctt ttgtttgtga caaaccttca tggatcgttt caatattcca
    42181 tttcatcatc tgatagtaag aatctccttg ttctccttct tttgctatgg aatcagtgaa
    42241 cagtttatca tagattttca aacccgtttc ttttgctacc cgttccatgc ttcttgagtc
    42301 tacactggtt tccacgaata aaaccggtac ttcttttgct cttatctgat caatgatcgc
    42361 tttcatttga tcaggcgtac cttgactttc tgtattgatc tcccatatat aagctgctgg
    42421 caaatcatat gctcgtgaaa aatacttgaa tgccccttca cttgttacta ataatttttt
    42481 gttctcttcg atagaagcaa aactttcctt cgcttgtgta tctaattctt ttagtttttc
    42541 cacatatgct tctgcatttt ttttatagag tgtcgcattt tctgcatctt tttcagagaa
    42601 tatacgtgcg atttcctcca catattttat tccgttagat aggtctagcc aagcatgggg
    42661 atctgctttt gtatgttctt caccgctagt taaataaaga ggttctacat ttttgctaac
    42721 tgcaaaataa tcttttcctt cttctttttt agccgtttcc atcaagttat cgaaccagct
    42781 gttacctgtt tcaagattca aaccgttgta taaaataaca tctgcatcgc ttgccttttt
    42841 gatgtcttct ggtagtactt catattcatg cggatctgtt ccgactggta cgatactgtg
    42901 gatatctatt tgatctgttc cgacctcttt tgccatgtcc gccaagatcg aattggtagc
    42961 cacgactgac aacttttctt tacttccttc atccgcttgg tcagaagcct gctttccgca
    43021 accggctaat accaagaaac taactgccaa gattaaaaag agtgattttt ttgttttcat
    43081 ctatctgctt cctttctgaa ataaaattcc ttttgttgga gaaaataaaa aagcaaggat
    43141 aaaaaatgcg gtacatgcta acacgatcgt agctcctgat ggccaattgt aactataact
    43201 gaaaaacaca ccgacaactg cacttagcat accgattcct gcagctgtaa tgatcatttt
    43261 cagtaaatgg ttcgttagca agtaagccgt ggcggctggt gtgatcaaca tggcaatcac
    43321 caagattgtt cctactgtct gtaaactgac tacagccact aatgtcaaaa agaacatcaa
    43381 aagataatgg atcagcgatg tgttcagccc ataagctttt gccattgtcg gatcgaatga
    43441 tgtgatcttc aattctttat aaaagaaaaa gacaaatatc aagacgattc cactaacgag
    43501 tgctgtcagc aacaaatcgc tttccctaac agctaagaca ttcccaaata aaatatgata
    43561 caaatctgtc gcactctggg catatgaaat caagataatc cctaatgcaa aaaaggagct
    43621 aaagacaatt ccaatcgctg tatcattttt caatcggctc cgctgagtca cgaaaccaat
    43681 cactgctgct gttagcatcc caaaagcagt ggctccaaag atgtaactaa aaccaaacat
    43741 ataagaaatg gctacacctg gaagaactgc gtgagagata gcgtctccca tcaaagacat
    43801 ccctcgtaaa ataataaaag aaccgatgac tccagaagca agtcctacaa gcatggaagt
    43861 gaccaaagcg tattgcagga attgataatt cattaatcca tcaataaagg attggatcat
    43921 tttttctctc ctccttttac taaatgtctg atgatctcac cataggccaa ttgaaggttc
    43981 ttcgatgtaa agacttcttg tactggaccg gaagcgatca gctgtttatt taaaagaata
    44041 acctcatcga aatattcttc tactttatga agatcatgat ggacgatcaa aatcgttttt
    44101 cctgaattct tcaattcctg caagatgtca aagatctttc gttcacttaa cgcatcaatc
    44161 cctacgaatg gttcatctaa aaagatccat tctgctcctt gggctagagc tcttgcaata
    44221 aaaactctct gaagctgtcc acccgatagt tcgctgatct gtcttttggc atattcttcc
    44281 atccctactt ttttcaaagc ttgtcttgca cgttcttttt cttttttccc aggtctttgt
    44341 ccaattcgta aagatggata tgttcctaaa agtacaacgc ctaatacatc aattggaaag
    44401 gaaagatcca attcacttcg ctgttccacg taggctattt ttttacggac tgagtttacc
    44461 gactgtccag aaaaggtgac tttaccagta gtagctggta tcaactctag caaagatttc
    44521 ataaatgttg attttcctgc accgtttggt ccgataatac cggtaatttt ttgtggattt
    44581 atcgtcaagt tgatagtttt caaaacagtt ttcccttgat aagcggctga aaggtcttga
    44641 attacaatgg ccgtacgttc caaatcttac actccttttt aggtttacct aactttttat
    44701 ttaaatatag tttaactaaa atggaaagca ttttcaagta gaatgatgaa aaagtttttt
    44761 tcgaattttc ctcgcatttt atgaaaattt tctgtagaat cacatcaaga gttcaataag
    44821 gctaacctaa taatgaaaaa catgtgttga aacgtttgat tattttttct tgttgactca
    44881 acaacgaaag gagtttttgt atggaagaag aaaagttaac tcgaagtctt agtgcccgtc
    44941 atatccaaat gatcgcacta ggtggaacga tcggtgtcgg tctgtttatg ggtgcctcct
    45001 caaccatacg ttggacaggg ccctcagtga tgggagccta tgcgattgca ggattctttt
    45061 tgtatttgat tatgcgtgct ttaggcgaaa tgctttacgt tgatccttcg actggttctt
    45121 ttgcaaatta cgcgagcgag tacatccatc ctgtggctgg ctacttgacc gcttggagta
    45181 atatttttca atatattgtc gtaggaataa gtgaagtgat tgctgtagga tcttatatga
    45241 attattggtg gcctgatttg ccggcaatta ttcccggaat cattgtcgtc ttgtttctta
    45301 tgctggcaaa tcttatttct gtcaaggcgt ttggcgaatt agaattttgg ttttcgatca
    45361 tcaaagtcat aacgatcatt ttgatgatca ttgcaggtct tggggtaatc ttatttggat
    45421 ttggtaatca tggtcaggca gtagggattt caaatctttg gaaaaatggt ggattcttca
    45481 ctggaggcgt caaaggattt ttctttgcat tatcgatcgt tgttgcttcc tatcaaggaa
    45541 tcgaactgat cggaatgact gctggtgaag cagagaatcc taaaaagacg atcattgaag
    45601 ctgtccagtc cactatcggt cgaatcttga ttttttatat tggtgctatt tttatcattg
    45661 tgagtatcta tccgtggaat caattgagtg agattggttc accttttgta caaacttttt
    45721 ctaaagtcgg cattacattt gctgcaggac tgattaattt tgtcgtgatt acagcggcat
    45781 tatcaggttg caattcaggg attttcagcg caagtcgtat ggtctataca cttgccataa
    45841 atggaaagat gtctaaaaaa tttctgaaac tatctcgtca cggtgcgcct ttttatcctg
    45901 tcgtagcaat ctcaggtgga atcttaattg gtctgatctt aaattatctt ctaccaatgt
    45961 tcatcaacca gtcagaaaat attttcgttt tcgtatacag ctcaagtatt ttgcctggta
    46021 tggttccttg ggtagtgatt ttgatcagcg agattcgctt caaaaagaag cacgcagata
    46081 aaatgcatga tcatcctttt aaaatgccac tatctcctta tacgaattat gtgacacttg
    46141 cttttttagc tatcgtattg attttcatgt ttatcaatcc tgaaacgaga atctcattga
    46201 ttattggact cgttttcttg ctttacatga cgatccatta ctttgtacgc gaaaaaagaa
    46261 ccggaaaata aaaaaagaac gaagcttcac cttagtggaa gcttcgttct tttttattgc
    46321 ttttatcaat tcaattttag cgtctcaccg ggagtcagga aataattctc catgctcatc
    46381 ccattcagcg acaagatcgt ttctactggt acgccagttc gttcggctat ttgatttgga
    46441 ccttctcctt ccaaaactac ggttgtcgca gcctgtgaag tcgcgctgtt ggtattagtt
    46501 tcagaggaat ctgttgtgtc ttgtgttgtt cgagcttgca tttcagaagc ttctaccgac
    46561 tctgctgtct cagaatcaga agatgttgtg tcttgtgtgc ttgttgactc tgcttgaaga
    46621 gaggcgctag tctgcaaata aatatcggaa atcttagcga catgattcgt ataattagcc
    46681 gataattgat tgatcaaagt atctaagtct acaccgtctt tattttctgc tgcctgatac
    46741 gctgcacgta attttctgat cccttcgtcg gagattgctt gaagctcacg tgctttttgg
    46801 ttagcaactg ctgataaacc agctattccc tcttgatttc cctgaatttc tgtctggtat
    46861 tcttcaatca atgcaggcgt cttttcttcg atctttttcg aataatcatt tacgattgct
    46921 tgaagttgct ctggcaaacc atttgctgtt ttttcttcag aagtttgtgt ctcttttgtt
    46981 ttagttgatg actcacttgt ttgtttcgtc tctgttttct ttgtcgttgt ttccgttatg
    47041 gtcgtacttt tcgcttctga cgaatttttt ccatgaaaat atagacccgc aactcctcct
    47101 gctatgatca aaaacacggc aactgctgcc ccaatcatga tcatccgctt acgagctgcc
    47161 tgtttttgct gtcttcttcg ggtcatccga acattttctc cccactcatt ttcatcatac
    47221 atttttttca ccaccaattt ttatgtataa tagcgttatc aaacgcttca tattcttatt
    47281 gtagcaaata attgtcgctg tgcacatttt tatctttgtt atttataaga aaatttcacc
    47341 agttgagatg attccaagat tgtccgtcga tttctccttg cagctgcatt gttcgttcgt
    47401 ctatctcagc catcagtttc tttatttcat ataagagcgc acggtcttga tattctgatt
    47461 tttcgcttaa ttcatctaat tgttggatca gccaatcatt tgtcataatg aagttccttt
    47521 catttcttgc agtgtaaagg tcaagtcttc gatctgctct tttgcctcag tcagtgcttc
    47581 tacgatatat gcttgttttt cagattcttc ttcctgcatt ttctgatttt cctgtactct
    47641 ttgaataaac cagtcaatcc gctgagtcag cgatggtttg ccttccaatg agagatactg
    47701 ctgataagca attagcttct cccaacgttt ttcctgggat aaatacataa attgtgctaa
    47761 aacgaatggc ggaagatagg tcatatggaa tttatttgcg attcgctgat acggcctcaa
    47821 aaattcggaa atcgtatatt cttcttttcc tccagcttgg tattcttttt ccgccacacc
    47881 tgttgtcaat acgattccta gttctttccc ttttaataca tgtgctgctc gttccaagac
    47941 attatcttgc caaattttta aatgagcagg agaactatac cagtaaaacg gaaactggaa
    48001 aatgatccga tcatgctggg ccaagagctg tagttcagtc tctatatcta tttttccgtc
    48061 tggataactt gattccaaat gatgccaagt agctggtaaa ccagcagaag cttctttgaa
    48121 aaattgctga tttaaagatt cttcgatcgt tggatggcta atgatgataa gtgtacgcat
    48181 cccttttcct cctttttagt cttcgcttta cagtgtagca taaccgccac aagtttcttt
    48241 tgccgatgct tgtcatttta accagactaa tcaggttgtc caagaaactg cggtttgttt
    48301 ttccaactgt tttttcaagg tatccaaagc aatattctta aaattttcgt ttttcatacg
    48361 tttacgatag ttcgtagccg tttctccggt actctttttg aacattcggc taaatacgac
    48421 tagactattg aaccctactt gataggcaat actttccacg gattcatccg tcagagaaag
    48481 aagaaacgcc cctcgattga tccgtaccct gatcaaatat tccttgatac tggtacccat
    48541 atgatttttg aatactcgcg acaaatggct ccgattgacc gctaaatgct gggcgatttc
    48601 ctcaacagac aatttttcag cataatgtgc ctcgatgtat ttgactgttt caacagctaa
    48661 agaggaatga accttttgta caccaccgct gacctttccg ccatctttta aaaggactga
    48721 taaaaaacgg taggttaatg ctgttaactg taattcattt tccaaggttt cttccgtatc
    48781 tttcatgcat tctaaaataa tgtcgatata tttttgtatg tcagaagaga ccatcgtgta
    48841 ttggtcttct ctgaaaagcg actgccgaat gatttcttct gcagcactcc caccaaatga
    48901 aagccagcaa tacatccacg gatcttctcc atctgataag taaaacgtcg agtctcctgg
    48961 acgaatcaag aacagatcac cttttttcaa ttggaattgt tgatttttca catgataaga
    49021 tcctttgcct tccatcacga catgcaggat gtaatcattg cgaatagcag gaccaaaaga
    49081 atgaaacggc atcgttttag agaaaccaca aaaatcaaaa tacaattctt tttctacttg
    49141 ttgatgttcc ttttttaaaa aaatcgattg ctccacgtct atccctcctt actcctttag
    49201 tataatgcaa caaaaagtta aataaaagtc acagaaatat attaaaaagg aagcgatttc
    49261 ttgttaagtt taagtcatta aaagatcttt gttattcgtt gtaaccggtt tacttagtat
    49321 tatatattga acaggagagg aatacagatt atgaaaagca aaatgatcca acgtgtgtcc
    49381 tacgctttag gcgcttttgg acacgacaca ttctatgtta cattaagcac ttattttatg
    49441 gtattcgtga caagccagat gttcgcagga acagataagg caacgaatgc aaaaatgatt
    49501 gctacagtta caagcttagt tgtaggtatc cgcttagttg aaattatttt tgatcctttg
    49561 attggtggcg tcatcgataa tactcgtaca aaatacggta aattcaaacc atggttagtt
    49621 atcggcggcg ttgttagttc cgtactcttg gtagcaatct ttaccaattt ctttggctta
    49681 gctactcata atacgacttt atttatcata ttatttgcaa tcgttttcat cttattggat
    49741 agtttttatt catttaagga tatcgctttt tggtcaatga ttccagcttt gagtacggaa
    49801 acaaaggaac gtgaaaaatt tgctacgttt gctcgtttcg gctcttctct aggtgcaaat
    49861 ggtactactc tagtcgttgt ccctatcgtt actttcttta cctatcttgt tactcataaa
    49921 catattgaag gcgcttcagg ctggttctgg ttcggtgtga ttgctgctgt tatttcaggc
    49981 ggtactgcga ttatcactgc cttgggcaca aaagaagtcg atactgcgat ccgtcagcaa
    50041 aatgaaaaag ctgggatcaa agatgtgttc aaagcagttg ctcaaaatga tcagttgatg
    50101 tggcttgccc ttgcttatat cgcttatgcg attgccaatg tagcaacaac tgccgtatta
    50161 ttctacttct tcaaatatgt acttggccaa ccaactgaat tttggatcgt tggtctagtt
    50221 gctgccttac ttggtttagt agctgttcca ctattcccaa tgcttacacg cgtcatctct
    50281 cgtcgctacg tttaccttgg agggatcgca atgatgattt cttcttactt tttctttacg
    50341 atcgcaggtt caagtttgcc aatcgttatc atcgggctag tattgttcta cttccctcaa
    50401 caattgattt tcttatccgc tttgatgacg atcacagact ctgttgaata tggtcaatgg
    50461 aaaaatggta tccgtaacga agctgttact ctatcaattc gccctttact agataaaatc
    50521 gctggtgctt tgtctaacgg gatcgtaggg tttgtcgcta tcgcttctgg gatgactggt
    50581 aatgctactg ctgctgatat cactgctcat ggcgtaacga ctttcaaagc ttatgcattc
    50641 tatgctcctg ctgctttgat gattttctcc gctattattt ttgcttggaa gattacattg
    50701 acagagaaaa aacatgcaca aatcgttgag aaattggaaa acaaattggc tccagctgat
    50761 acaatggaag atgaacttca cgatgcagta gttccttccc attaagaaag gatcttgaaa
    50821 atgaagatta cagaaaaaga ttttggccaa ggttatcact tgataacctt ggagaacaaa
    50881 aacaagctag cactttctat ctctgattta ggtgctagaa tcgtcagttt aaaatcaaat
    50941 gatcgagaat tagttttggg atttgacacc gctgaagaat atatcgaaaa agatccgtat
    51001 atcggtgctt ctatcggtcg caccgcaggg agaatcgaaa atggccgctt ttctctcaat
    51061 ggaaaaactt accaattagc cacagatcca aaaactggac ataatctgca tggtggtgct
    51121 cctggatttg aattaaaaaa atggtcttat gtcattttga atggcgaaaa tgaagcttct
    51181 gtcattttta ctactacaag tcccgacgga gaacatggtt ttccaggtac aatggatgtg
    51241 gaaatccgct atactttgac aaaagataat atctggcgag tgacctctcg cggcaccagt
    51301 gaccaagata ctttattcaa tccgacaaat catgtttact tcaacttgac gggagatgct
    51361 tctcagtcaa tcgatcaaca tgagctttgg ctcaatagcg aagcttatgc tcctttgcga
    51421 acagatagta ttcctatagg ggtaaaagaa aatgctgcag gaagtgcttt cgattttcaa
    51481 atacctaaaa aattagcgtc cgtttttgct agtgatttgg atcaaaaaaa tcttgttgac
    51541 ggaatcgatc atccattttt cttaaaagaa accggcttgg gtaaagaagc cgctcgcttg
    51601 actagccccg ataaaagaat ccaagtagat attgctacag atgcttctag tgtggttatt
    51661 ttcactgcaa atttcggcac agaaacacca gaaatgcgca accgaaaact ggcacatcac
    51721 ggtggcatca catttgagac tcaaacagct ccaggtgcag agcgcttctc agcatttggt
    51781 tcgattcatt taaaagccgg atcagtattt gaaacagtaa cagaattcaa aatcaaaaca
    51841 agaaaggaat aactcatcat ggaaatcaaa agaagcttaa acacaaaatt tgccgaaaca
    51901 ttcggaccta aaaaaactac acaatacttc tcaccaggac gcatcaattt gattggtgaa
    51961 cacacagatt acaatggagg ccatgtcttc cccgcttcca tcacttatgg aacgcaaggt
    52021 gtagcagcac ctcgagacga taataaaata ttagtttatt ccacaaactt tgacgatatc
    52081 ggtgtcatta gttttacttt agatgagcta gaatatgata aaaaagcaaa ctgggcaaat
    52141 tacgtaaaag gaatggtcct taaattaaaa gaagctggac actcaatcga tcatgggttt
    52201 gagttattag tggaaggaac aatccctaat ggtgcaggac tttccagtag cgcttcatta
    52261 gaattattag taggtgttgt attagaagat ctgtttgatt taaaaacaga tcgcttggaa
    52321 ctagtccaaa caggtaaaaa agtagaaaat gaattcatcg gtgtcaattc aggtatcatg
    52381 gatcagtttg ccatcggctt cggtgagcta gatcatgcaa tcttattaga tacaaatacg
    52441 ctgaaatacg aaatggttcc agtgaaacta gatggttatg caatcgtcat catgaatacg
    52501 aataagcggc gggaattggc agattcaaaa tacaatgaac gccgtagcga atgcgaagaa
    52561 gccttggcac gtttgcaaaa aactttgcct atctcttctt taggagactt agatgaagaa
    52621 gaatttgaat caactattga ccaaatcggc gatgatacat tgatacgcag agctcgccat
    52681 gccgtttctg aaaatcagcg gactttgaag gcaaaagctg aattagaagc tgggaactta
    52741 gaagcatttg gtcaactgtt aaatgattct caccactctc ttcgctatga ttatgaagta
    52801 actgggatcg aattagatac actagtagat gctgctcaaa aacaagaagg tgtcttaggt
    52861 gcacgaatga caggagctgg atttggtggc tgtgcgattg ctcttgtcaa agaagaaaat
    52921 atacctgcct tcaaaaacaa tgtctatgat gaatacatga aagttgtcgg ctatgctccg
    52981 gaattttatg ttgcacacat tggaaatggg acaacaaaga tcgatccaga agctgttcat
    53041 gtttgataaa aaaatcaacg atctcagcta aaaacaaata gataaaaata acctctcagc
    53101 aatttccaaa tgtgagcctt ttgctcgtat ttgaaaattg ctgtttttta ttgacacgga
    53161 aagagaacgt gtgttctaat tgtgttgaag aggtgattgt tatgtgggcg aatcaaaatc
    53221 cggtatttgt ttatgaaaaa gaaccttcaa gagatatttt atgtattgac tgcaaatctt
    53281 tttacgcctc cgtagagtgc gttgaaaagg atcttgatcc ccttacaact aaattagtag
    53341 tcatgtccta tccgtccgat tcgacagaaa cacggggtag cggattaatc ttggcttcca
    53401 gtcctgcagc aaaaaaagct tacggcatca ccaatatcag ccgtgccaga gatcttccct
    53461 tcccctatcc aagtgattta tatattgttg ccccaaggat ggcctattat atggaaaaga
    53521 acagagaaat caattctatc tataaaaaat atgcagacga agcgaaccat catgtctatt
    53581 ccgtcgatga aagcttcctt gatgtaacaa atagcatggc actctttggt aaaacaaatg
    53641 cgtacgacat ggctaaactg atccataaag atgtgtttga gcagaccgga atctacacga
    53701 ctataggaat tggcgataac ccgctacttg ctaaactagc cttagacaat ggctcaaaag
    53761 acggaaagca aatgtttgct gaatggcgtt atgaagatgt accagaaaag ttatggaaaa
    53821 tctcagatat cactgacttt tggggaatcg gcaaacgaac agcaaaacgt ctgaaacaaa
    53881 tgggtataca ttctgtttat gatttagctc acgcggatta ttatcacttg aaaagcaagc
    53941 taggggtcat cggtactcag ctttatgccc atgcttgggg catcgatcga agttttttgg
    54001 gacaagaata tacaccaaaa tcaaaaagta tcggaaatag tcaaatccta gatagagatt
    54061 atacaagacg agaagaaatc gagatcgtta tcaaagaaat ggccgaccaa gtcggcactc
    54121 gcttgagacg atctgcagca aaaacacaag tagtcagctt atgggtagga tactctttag
    54181 gctatcttga tccaaatggc aagaccgggt ttcaccagca aatgaaaatc gaagcgacaa
    54241 atgctagcag agatctagca gatgctttat tgaccatttt ccaccagcat taccactacc
    54301 aggatattcg gcgtgtaggc gtgaactgtt ccgacctcgt atacgcgaca ggtttacagc
    54361 ttaacctttt tgaagatcct gaaaagcaga taaatgaagc gaaagttgat tttgttgtcg
    54421 atacgattag aaaaaaatac ggctttcaat caatcatcca tgcgcatagt ctcttgcaag
    54481 gaggacgcgc catcgcacgt agttctctcg ttggcgggca tgcaggtggt atgagtggat
    54541 tagaaggtgg aaaagatgag acggacagca aaaaaattca ttgactataa cgagtatcga
    54601 gatcggccat ttggattaaa atgggggact gcttttgcga tggaagaact agtcaacggt
    54661 atcaaaaaaa tgaagaagaa gcacgcaaac agcctcttcc taaacaacaa atgacattgc
    54721 aagaaatcga tagtatactg acagaagcct ttttatctcg taaagcagta atcattcagc
    54781 tcaatttaaa agatgaattt ggaagactct tggatgaggt cgaagggcac ttcattggtg
    54841 aagcctatga agattacttt atgattgacc atcagccgat attgtgggaa gacgtacggt
    54901 atattgagtt aaaggaaaca aaaaaatggt ttgtgacatg agaggttgtg acagaagtga
    54961 tcaacttcaa aaaataagaa aggatttttc gaaaattgct tcttaaattt ttgtggaatt
    55021 ttggcttatt tttcgaagaa gcaacttctg gaacaacgta tattagatat cagaggttgt
    55081 gaaaaagtcg cttagttttg agtaacaagc caaggcgctg atttcacaac ctctgtctat
    55141 ctatccatct attcggtaag attcatgcat gacgtcttct ttatttagta tgtagagccc
    55201 tttttcttct gaccattcaa cataaatcag ctgttttaca tctgcatagc cttctttctt
    55261 caagttctcc ataagccatg cttcagtcaa ttgattggac tgaagcactt tatgctgaat
    55321 ttgcccttca tctacaagca ggatcgataa agctttgtct tcatctgatt ttttcaatac
    55381 actcatttga ccggcattct ctaggataac atgtttggca ttttcaagaa aaaagcattc
    55441 ttgctgtctt aagattgctc gcaactgctc catctcgata tggttatttg tcaactctgt
    55501 catgttgatg acgccgtctt tgattagaac agatggctcg cctttgaccc aacgattgac
    55561 cttttcattt ttttggacga tacgttcgat accataaatc attactgccc aaagcgcaat
    55621 ggcaaatagg acatgcccta catggacatt gtcatcatag gttgattctt ctaagatccc
    55681 acctaaaaca agcgtataga ctaaatcaaa cggtgtgatt tctgacatgg atttcttccc
    55741 aagcaaacgt accactaata ataaaccgat caacccgaac accagtttca ttgtaattga
    55801 aaaaatcatt ttttctcccc ctaactatct gttaattaaa ttatatatgc tttctcacga
    55861 acacgcatgg aatatacaag gaagaaatgt atccttttac taaaaaaagg actgtgacac
    55921 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactcc ttttttttat
    55981 gctctaaaat gatattttta cacaaccgtt acggttgttt tacgaaattg gacgagactt
    56041 tgccagttta atttctgttt tatccgttca ttattgtagt aatagatata gctatcgatg
    56101 gctgatttta attcctcgaa acagttataa actttcccgt gaaaaatctc ttgttttaat
    56161 aagccaaaaa aattctccat aagtgaatta tctaagcagt tccctttccg agacatgctt
    56221 tgaaagattt tgtactcctt caatttgttt ctataagctt tcatttgata cgcccaacct
    56281 tgatctgagt gaaacgtacg acgataggga caatcgttag tggtctggat agcttcctcc
    56341 aaaccttcca ttaccgctaa tgcattgggc ttttcagaga tacgatagga taaaatctca
    56401 ctattgaaca tatccataaa aggatctaga tacaatttct tttgacggat aatgcctttg
    56461 gcatccactt catggtattt aaattcagtt gtatccgtcg tgattttctg atggcagata
    56521 ctggtgtaga aacgacgatg gat
//
LOCUS       DASHWH010000010        55729 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.010, whole genome shotgun sequence.
ACCESSION   DASHWH010000010 DASHWH010000000
VERSION     DASHWH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.010"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..538
                     /locus_tag="U2R41_000615"
     CDS             <1..538
                     /locus_tag="U2R41_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8888048.1"
                     /translation="KKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNP
                     KECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKQKQTIERIF
                     ADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVD
                     KKLFQARVTSLEQLLSTV"
     gene            complement(579..1958)
                     /locus_tag="U2R41_000616"
     CDS             complement(579..1958)
                     /locus_tag="U2R41_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEM8888049.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1979..3526)
                     /locus_tag="U2R41_000617"
     CDS             complement(1979..3526)
                     /locus_tag="U2R41_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8888050.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3775..4554)
                     /locus_tag="U2R41_000618"
     CDS             complement(3775..4554)
                     /locus_tag="U2R41_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8888051.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4629..5087)
                     /locus_tag="U2R41_000619"
     CDS             complement(4629..5087)
                     /locus_tag="U2R41_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8888052.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(5099..6373)
                     /locus_tag="U2R41_000620"
     CDS             complement(5099..6373)
                     /locus_tag="U2R41_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEM8888053.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(6474..6788)
                     /locus_tag="U2R41_000621"
     CDS             complement(6474..6788)
                     /locus_tag="U2R41_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8888054.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6934..8430)
                     /locus_tag="U2R41_000622"
     CDS             complement(6934..8430)
                     /locus_tag="U2R41_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEM8888055.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8608..9087)
                     /locus_tag="U2R41_000623"
     CDS             complement(8608..9087)
                     /locus_tag="U2R41_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HEM8888056.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            9254..9403
                     /locus_tag="U2R41_000624"
     CDS             9254..9403
                     /locus_tag="U2R41_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888057.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(9463..9708)
                     /locus_tag="U2R41_000625"
     CDS             complement(9463..9708)
                     /locus_tag="U2R41_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888058.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9813..10586
                     /locus_tag="U2R41_000626"
     CDS             9813..10586
                     /locus_tag="U2R41_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888059.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10815..11063)
                     /locus_tag="U2R41_000627"
     CDS             complement(10815..11063)
                     /locus_tag="U2R41_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8888060.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(11162..11476)
                     /locus_tag="U2R41_000628"
     CDS             complement(11162..11476)
                     /locus_tag="U2R41_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888061.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11613..11759)
                     /locus_tag="U2R41_000629"
     CDS             complement(11613..11759)
                     /locus_tag="U2R41_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888062.1"
                     /translation="MILNNPEYIESGKAETVNYYYELPSEIQNYQIVIREADGYFVGL
                     PFTL"
     gene            11933..13207
                     /locus_tag="U2R41_000630"
     CDS             11933..13207
                     /locus_tag="U2R41_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HEM8888063.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(13267..13614)
                     /locus_tag="U2R41_000631"
     CDS             complement(13267..13614)
                     /locus_tag="U2R41_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888064.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(13607..13909)
                     /locus_tag="U2R41_000632"
     CDS             complement(13607..13909)
                     /locus_tag="U2R41_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888065.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(14125..15516)
                     /locus_tag="U2R41_000633"
     CDS             complement(14125..15516)
                     /locus_tag="U2R41_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM8888066.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(15501..16112)
                     /locus_tag="U2R41_000634"
     CDS             complement(15501..16112)
                     /locus_tag="U2R41_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEM8888067.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(16124..16312)
                     /locus_tag="U2R41_000635"
     CDS             complement(16124..16312)
                     /locus_tag="U2R41_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888068.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(16326..18971)
                     /locus_tag="U2R41_000636"
     CDS             complement(16326..18971)
                     /locus_tag="U2R41_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888069.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(18958..19176)
                     /locus_tag="U2R41_000637"
     CDS             complement(18958..19176)
                     /locus_tag="U2R41_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888070.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(19178..20221)
                     /locus_tag="U2R41_000638"
     CDS             complement(19178..20221)
                     /locus_tag="U2R41_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8888071.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(20376..20702)
                     /locus_tag="U2R41_000639"
     CDS             complement(20376..20702)
                     /locus_tag="U2R41_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888072.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(20710..20913)
                     /locus_tag="U2R41_000640"
     CDS             complement(20710..20913)
                     /locus_tag="U2R41_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888073.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            20988..21638
                     /locus_tag="U2R41_000641"
     CDS             20988..21638
                     /locus_tag="U2R41_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888074.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            21693..22859
                     /locus_tag="U2R41_000642"
     CDS             21693..22859
                     /locus_tag="U2R41_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8888075.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(22948..23340)
                     /gene="rpsI"
                     /locus_tag="U2R41_000643"
     CDS             complement(22948..23340)
                     /gene="rpsI"
                     /locus_tag="U2R41_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM8888076.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(23356..23799)
                     /gene="rplM"
                     /locus_tag="U2R41_000644"
     CDS             complement(23356..23799)
                     /gene="rplM"
                     /locus_tag="U2R41_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEM8888077.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(24318..24785)
                     /locus_tag="U2R41_000645"
     CDS             complement(24318..24785)
                     /locus_tag="U2R41_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEM8888078.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(24905..26443)
                     /locus_tag="U2R41_000646"
     CDS             complement(24905..26443)
                     /locus_tag="U2R41_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEM8888079.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            26523..27209
                     /locus_tag="U2R41_000647"
     CDS             26523..27209
                     /locus_tag="U2R41_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEM8888080.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(27410..27937)
                     /gene="msrA"
                     /locus_tag="U2R41_000648"
     CDS             complement(27410..27937)
                     /gene="msrA"
                     /locus_tag="U2R41_000648"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM8888081.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(28021..28698)
                     /gene="pgmB"
                     /locus_tag="U2R41_000649"
     CDS             complement(28021..28698)
                     /gene="pgmB"
                     /locus_tag="U2R41_000649"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM8888082.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(28695..29825)
                     /locus_tag="U2R41_000650"
     CDS             complement(28695..29825)
                     /locus_tag="U2R41_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8888083.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(29825..32008)
                     /locus_tag="U2R41_000651"
     CDS             complement(29825..32008)
                     /locus_tag="U2R41_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM8888084.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIAPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32005..33030)
                     /locus_tag="U2R41_000652"
     CDS             complement(32005..33030)
                     /locus_tag="U2R41_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8888085.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(33075..33902)
                     /locus_tag="U2R41_000653"
     CDS             complement(33075..33902)
                     /locus_tag="U2R41_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM8888086.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(33899..34966)
                     /locus_tag="U2R41_000654"
     CDS             complement(33899..34966)
                     /locus_tag="U2R41_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEM8888087.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(34992..35828)
                     /locus_tag="U2R41_000655"
     CDS             complement(34992..35828)
                     /locus_tag="U2R41_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8888088.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(35825..36694)
                     /locus_tag="U2R41_000656"
     CDS             complement(35825..36694)
                     /locus_tag="U2R41_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8888089.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(36764..38047)
                     /locus_tag="U2R41_000657"
     CDS             complement(36764..38047)
                     /locus_tag="U2R41_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM8888090.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMTGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(38061..39746)
                     /locus_tag="U2R41_000658"
     CDS             complement(38061..39746)
                     /locus_tag="U2R41_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEM8888091.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            40021..41046
                     /locus_tag="U2R41_000659"
     CDS             40021..41046
                     /locus_tag="U2R41_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888092.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(41641..45315)
                     /gene="rpoC"
                     /locus_tag="U2R41_000660"
     CDS             complement(41641..45315)
                     /gene="rpoC"
                     /locus_tag="U2R41_000660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEM8888093.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(45373..48990)
                     /gene="rpoB"
                     /locus_tag="U2R41_000661"
     CDS             complement(45373..48990)
                     /gene="rpoB"
                     /locus_tag="U2R41_000661"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM8888094.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            49718..50686
                     /locus_tag="U2R41_000662"
     CDS             49718..50686
                     /locus_tag="U2R41_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888095.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            50704..51609
                     /locus_tag="U2R41_000663"
     CDS             50704..51609
                     /locus_tag="U2R41_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888096.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            51606..52412
                     /locus_tag="U2R41_000664"
     CDS             51606..52412
                     /locus_tag="U2R41_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888097.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            52548..53537
                     /locus_tag="U2R41_000665"
     CDS             52548..53537
                     /locus_tag="U2R41_000665"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEM8888098.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(53854..54378)
                     /locus_tag="U2R41_000666"
     CDS             complement(53854..54378)
                     /locus_tag="U2R41_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEM8888099.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            54665..>55729
                     /gene="scm"
                     /locus_tag="U2R41_000667"
     CDS             54665..>55729
                     /gene="scm"
                     /locus_tag="U2R41_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEM8888100.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        16254 a        11599 c         9618 g        18258 t
ORIGIN      
        1 gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg attgttatat
       61 ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt accgagaata
      121 caaatccaat ccaaaagaat gtgtgaactg tcctctgcta aaccaatgta ccaaaagtaa
      181 aaatcatcaa cgagtcatca cgagacatgt ttggggagat ctgatggatg aagtggaaca
      241 tcttcgccta acagatctga ataaatcaat ttacaaaaaa cagaaacaaa cgattgaacg
      301 aatatttgcg gatgcaaaag aaaagcatgg gatgcgctgg accaagtatc gaggcttgga
      361 aaaagtcgcg acgcatacga tgctagtgtt tgctgcaatg aatttaaaga aattagccac
      421 ctggctatgg aaaggcaagg agcctttatt tttttgttca aaaattcgaa acgaagtaga
      481 taaaaagctg ttccaagctc gagttacgag tttggaacag cttttgtcta cagtctgaat
      541 catttcttcc gttttaaaga agaaatgatt ttcatagatc aacctttaac taaagctaga
      601 ttcttttcta aaaagaatgg tgtgtagtag cccacaccgc tggacaacgg taaaacttca
      661 tccggcaact cgatgcccag ttcgttacgg atgtactctt ggcgagaaat gattcgcgac
      721 caaacatttg gatattcttt ggctagttga tcacgaagcg actcatcagc tagcgcaatc
      781 ccactctcac agcttgcgcc ggcatagcca gcaactgatg gaataatatc aatttgtagt
      841 aacattccgg acttaagagt aatttctgag tcttttttca ttggcgaaga catccactct
      901 tcatcagccg taagatgccc cggatttaag tgccatttgt attccgattt aggcaagact
      961 ttgcctatta ctgaatatag ctctttacct tgcattccta ttttgatatt ttccaaccat
     1021 gcgaccacgg cgtcgtagta tggagctgca actttttcaa tgtaatcctt ttgcccttct
     1081 ggtaggtcat ctgtcgttgc aactgcatag ccacttcgac ttgctaatcc gcctttaaag
     1141 cccgtagtaa ttgacatttt atcgcctagt ttaacttgtt tatcagttgg atatatatag
     1201 gcgttctcaa accgttcccc agttgcagca attggaatga cattatttgg ttggccgtac
     1261 ttaactaagt ggttaccaat ttccatttct gtttttccta cctctacttc atcaatagcg
     1321 ttcatcacac aatctgaagc caaggatgag ccaaattcat aatatgccac ttcattagca
     1381 gtatttaatg agcgtactcc gtaatcactg tgaatgaaca ggtcggtgcg attgctaacg
     1441 gcatcttccc ctactaattc tacaatcgaa cttacaatga atgccggcaa atcttggatt
     1501 tggtagttat cctcttctga agtaaacatc ttccaaccaa caagcccaac tttcttacct
     1561 ttactgattt tagcattggt caatacttca gttagtggtt tctcgttatc catcggttga
     1621 tccggtaatg agaagagtgg cgtgtggatt aattctgcct caattcttga atagtccacc
     1681 attttagtat tttcatttcc tagaactaag aaagcttcac ccgtctgatg taataccaac
     1741 aagctttctt caaagcgagt aacaaaacct gttaaataag agaagttccc gccatgttcc
     1801 aaatcacaat agattacgag ggtatctatc tctgactttt ccatcgctgc aagtaccttt
     1861 tctttacgga acttcattgt ttcatcagtc aattgatata ttttagcttc attatctttt
     1921 ttaggtgggt taactttttt tagagttacc ttagtcatat tgtcactact ttctgttttt
     1981 atttacgctt catttcattt ttcagagatc gagttgcgtt ttccacttgt cctccatata
     2041 ccacttgtaa attattttcg ttaattttaa cgattcccat tgagccggtc tttttcaaga
     2101 tatgttcatc aattgttgct gtatctttaa gaactagtct cagccgagag atacagttat
     2161 cgatttcttg aatattacca gcaccaccaa gtccttgcaa gattagtttg ccaagttcgt
     2221 tttctgtcat ttcttcgtcc tgatcttcca acacatcatc gttggatcga ccaggagttt
     2281 tcacatcaaa tcgctcaatg acatacttaa atacaaagta atatgcaact gcataaacaa
     2341 ggcccaatgc tacaactaag taccaacgag tataagtacc ttgtaaaatt ccgaagatag
     2401 ccaaatcaat aaatccacca ccggcattcc caactgcgac tccgaataga ttcattaaca
     2461 taaaggataa accagccatg aatgcatgga agacccatag aatcggtgag ataaagataa
     2521 aggcgaattc aattggctca gtaatcccgg ttacaaaaca agtagccgct gctgcaatca
     2581 acatcccttt aatctttgga cgttgcgcca aatatgaagt tttgtacatt gcatagcatg
     2641 ctgccggcaa tccgaagacc ataaatggat gagtaccttg tgtcaagaag cgagtagcac
     2701 ttctcaaaat atccatgtct tttactggca atgctaattc agtattgaag atggttaatg
     2761 ctccagaaac tgtttcacca ttgatagttg ccgtaccgcc gattggtgta aatcgaatca
     2821 actggtttag tacatggtga agacctgttg gaatcagcag gcgctctgag aagccatata
     2881 aaaaagttcc aattacgcct gacttaccaa tcaaaccgcc taaagcattg atagctgtat
     2941 tgacaaaagg ccagatgaaa aatgaagcga ttgagatgat tggaatagtt aggatgatca
     3001 taattggaac aaaacgattg ccactaaaga aactgaaagc tgctggtaat tgttttgtgt
     3061 gatagcgatt gtggatcatg gctgtccata atcctgcaat gataccgccg aaaacaccca
     3121 tacgatatgt gaatgtgcca agtaaagttt catacatatt ggaagtttct actgctttga
     3181 tttgactcat tccgtcagac atcaatgttt caacagaagt gctttccgct gtgatgcctc
     3241 taagctgaag cagataattg ataactacat ggaacagaat aaatgcaata accactgaaa
     3301 atgctgcaat ttccttatct tctctagaca tcccgattgc tactgaaata gcaaatagaa
     3361 taggaagtag tccaaacatc gtaccaacaa tagcctgtac tccaccaaca aatccttgga
     3421 tcacggttgc attagcgaag ctttctccca ccaaagtagg attttgtaaa attgaaacga
     3481 ttcctagtag tagtcccgat gcgacaataa tagacaacgg acccattaat gatttcccta
     3541 acttctggat ataagttctc aatataaaca ccttcttctt tttagttgat ataaacgttt
     3601 acatcccttg tacaacgaaa gtatagcacc ctgaaaaagc gctaacaata cgataggtct
     3661 gataaaaatc aacattcagc acttggttgt tcattcaaaa cgtgaatctt caatcacaga
     3721 aaaatacgcg ctatcaagca cgtattttag gtgtaaatat atgcatcaac tttattaatc
     3781 aacgctactt tgtttgtaat agttaatata cccttcatac agtaagctaa gagcaagatt
     3841 caaggtgcag aatgatgagt tgttcaactc attcacatca ttaatgttgg tcacttgata
     3901 gtataggcta tagtctgaca gactggctaa gttattttga ccaaatcgtg taactgagac
     3961 caactgtgtc cctttcatat ggattcttct taaagtatca tcaatccgat caacacgtcc
     4021 ggacaatgat atgacaaata gtaaatcatt cgaggtcaaa gcttttgaaa tcaggtctaa
     4081 ttcattcttt tccggtacaa cgataatgta gattcccaag ttccacatgc agcgtgcaaa
     4141 ctcattgagc atcattcttt gtccatatcc tgttgcgtag gcaaatatct tatctgcctt
     4201 tctaatcgtt tcatatatat cctcaacact tttatcatct tcaaacaatt tggttgtcgc
     4261 ttgaacatct ccgagtaata cttccggtaa aaacaaacta cttttatcga ctctggtttt
     4321 ttgcatgcgt gcaaattcct ctttaaggta atatcgaaat tcgccatatc ctttgaatcc
     4381 gagtttctta gttagtctaa taatcgttga gtcagacaca tttgcttgtt ttgctaattc
     4441 cgatacagtt tgactgatgc tgatttccat attttgagtg ataaaactta atacataatc
     4501 atcattctca ttcaatttac tgtgatgttc atttactaat tgctccaatg tcataatcag
     4561 acctccaaaa ataataaaaa agattgctct ctcattttaa tacgaaagca caatcttttt
     4621 aagtactatt aaattttgaa aagaagttct ccttcagaaa cctttttttc agagacttct
     4681 agtttaatac ccttatcttc gggaaataga accataacaa catctgacag tttttccgcc
     4741 ttgatttggt cacaatcaaa aatcaccaat gactcgccgg atgatactcg ttgaccttct
     4801 tcaacgagca cttgaaaccc gactcccttt aactgaacag tatcaattcc aatatgaatc
     4861 aataaatctc taccttgatt actagtcaat gtgagtgcat gctttgttgg aaatatcgat
     4921 ttaatgacac cgtcaatcgg tgctaccacc ttagcgtttg ttggttcaac agcgaatcct
     4981 tcgcccatca tcccttgcga aaatacagga tcgcttactt ggctaagggg aatcagattt
     5041 cctgatactg gagcaactaa tttttctttt cgactaaaaa atcccattga cttactcctt
     5101 aatcttttga aatgtccatc acaaggttta aaagaacatc cgcccctttt ttgacatcct
     5161 caaaatcagt ccactcatca ggatgatgtg taatgccacc tttggatgga atataaatca
     5221 tatttgttgg gacaaagtcg gtcatcatca tcgcatcatg tccagtacca ttgttaatga
     5281 cttgatagct atagcccaac tgatcgcaaa tatgtttcat cttctcaaca ttaccttgat
     5341 ccataacact tggatttgta ggattactgt agttaattcg agccatttcc tccagctcag
     5401 ttctaactcc agtttcccct tgaatcactg ataactcatt caacaacttt tctttaatct
     5461 cttgaggatt agttgtcttt gtatcaaagg ttctgatttc gattaaagct tcgacaaagt
     5521 cagggacaaa ttgatttgag ttcggtttga ttgtaagttt accgacggtt ccggcaatcc
     5581 cttcaccaag attcttcatc cactgttcaa caaaaagaac aaatttagaa gcagcaacta
     5641 aagcatcttt ccgtttattc attggagcag tcgagtcctc agtttctcca taaaaacgtg
     5701 ctttaaaacg gccgatgcca ggaataaatt caatcaatcc gatatctttg ccttcatctt
     5761 ccaggactgg tccttgttcg atatgaagtt caataaaatt tttgattgtt tttggatctc
     5821 gcttagcggc ctctaaatct ggttccactc cataagcacg aagagcttcc aattttgttt
     5881 gtccatggcg attttttact gtttccaact cactcatggt taatgttccc atcatagctc
     5941 gtgaatttga gacacccgta cttggcccaa atgtctcgcc ctcctctgca ttcatcagaa
     6001 ttaggtcaat tgggtaatca ttttcaaaat tattttcttc cagaatccgc attgtttcca
     6061 gtgcagtaac aacgccagcc actccgtcaa atctgcctcc attaacaact gaatcataat
     6121 gagagccaat catcactgat ggtgcatcag cctttttacc ggctctacga ccaaataatg
     6181 tagagaagcc gtcttcccaa atctctaaac ccagttgttc catttgattg atcaaataat
     6241 ttcttgcttc acgatcttct ttagagtaag cagagcgggt cactccttca cctggagttg
     6301 cattgtactc ggctaaattt tcgatatcct tttttatacg ctcaaaattt gtcttcataa
     6361 ttaaatcctc catttcctac aagtgaatgt tagcacccat agaaagcggc tacaatataa
     6421 tcaatgaaga ttgaatcgat attcgaaaac tgaatattgc ttcggttaat gacttagaaa
     6481 tcattatcgt agattccacc ataagaactt tttctcgtct cataacacaa ctcgagagtg
     6541 gattggttta aaaaactctc gataaatgcg tccactttat tgacacagat ccatggatcg
     6601 atcgcacctt gaaatttaaa cccagaaata gtcccatcat cttctatacc gattaccagc
     6661 ttacctccat cggcatttgc aaacgctact atgtgtttta taatatctct aggcttaatc
     6721 cgtgcacttt ttctatcaaa atactgatct tctttttcac ttataaactt ttgaatctca
     6781 taatccattt aaaatttccc ttcttaaatt tttttattat agagccgtat attcaatttc
     6841 cacaaaaaaa taatctaaaa caaaacagac accttcattt tgatatgttc tcccacatat
     6901 gaatcggtcg ctatacagca ttacatatag taattattca tactgcgaaa taaatagctc
     6961 caaatcttta gctggcgtag ttaagcttaa attcgaaatg aaagtttcat tgactattcc
     7021 actttcatca aatcccttct tcaaccaatc agagttccac aacgtaaatc ctttaggatt
     7081 agaaactttc ttacataatt ttgcactata ggatttctct cttgaatgaa ttaaagaaag
     7141 tctttgtaac caaatttcaa ctatatccgt attaggagta ttaacaaatt ttcttaagat
     7201 tttatccaag tataagttga tttcttctac agcaataaac tcaaaaagct tacctaaaat
     7261 cacaatacaa gcctccaaag ttctaggatt cttttgcatt atattaacaa gaatactaat
     7321 caattggtag atatcatctg gtcttttgtt taaagtagaa actctatgtg tatagacttc
     7381 acttagcgct ttacttaaac tcccgcaatt tggatatttg tctcctaaaa gctttacctg
     7441 tagcaaatgc ttttgaaggc taagcttaaa cttccactta ttattacttt tctctgtaaa
     7501 acttgcatgt ttaagatccc agtaaatttt atcaggctta attgcatcta aaattatatt
     7561 atccgataag aatgttttat ttgaatttag cttcaaattt agatccgata atacttcaga
     7621 taacaatctg ataatcactt cagcttgatc ttttcgaacc gaaaaaattc tgtaatcatc
     7681 tctgtaacga agtattttga actctttaat ctctgttttt tctatcttgt tcagtaacat
     7741 tttatcagca tacccaagta ctatttcagc tacaaaatcc atcaatacat ttccttgagg
     7801 tataccgttc gtttgtccat tttgtaaata tcgtatttta gaatctatta agtttccaac
     7861 gccttgagta tcttttctat gtgtcttagc ccattctttt gaatgtactg cccacgggat
     7921 agtatgtgtg tacattgagc tataacaatc ggtaatatct gaatgaatgc agtagtcata
     7981 ctccaaagca taactaatct gtgcttgttc aaggttctcc caccaattta aaatagtttc
     8041 cgcagtatcg ctctttgaag aaatagattc cacaggaagt gatatacatt caattcgctt
     8101 gtcggcttgg aattcttcaa acttattttt gatgtgattc caattatttt tctgtgtaat
     8161 cccgtttact aagtctacat atactaatgg atgtaatatt tgaaggggtc tccaagcata
     8221 attaccatct ttattagcta atattcgatg attaacatca actgcctcag agagcgatct
     8281 cttatgtcta actagttcag taaatggttt gttttttaat aaatccactg ctttctcaat
     8341 gcctactgat aaatcaatat agttgggcaa cgatgtacta aaaaaacttg atgtttttaa
     8401 aaagaattgc tttgcttctt gatgattcat ctctaaaata ctttttctag tcctagtcaa
     8461 attcaaaatc tcctagatca attaagttgt aatgtaagag gataccctaa ggttacacac
     8521 cctatttact attataccag tattcattcg tgatacataa gggtgtaaca ttaagaactc
     8581 tatctaaaag actacaagta gtcaccttta attgtgagta attttcttta tccaatcctt
     8641 gtgcaacctt gtcgtcaccc cttgtatatc cccgtacccc ggatgtttcg gaatatcaaa
     8701 aatgtgctca aattcctgat tttcatcagt ctcccactca tgattcaacg gttcatgcca
     8761 ctctataaac cttgaccata aaatgttctt tctataaaac tcaataggaa tttcaaaaca
     8821 gtaaaaattg tatttgctaa aatctgtaaa attatcttta cgagcatact ttaaatggca
     8881 ggtatctcgc cacaaccaaa ttaaatctcg atccaatggt aactgaattc gtttcgtata
     8941 ctcttcttca agccaatgaa gtgcaccaac tgcattcgga ttatcgaaaa agacagataa
     9001 ttcagtatag cgtttctttg gataaataaa tccttctgaa tcaacagatt gcaaaaaatc
     9061 tactggttga accgtgtaca ctttcattta ttcgccacct ttttttgact acttatagtc
     9121 atttaccaat ttgaaatctg catacttttt cattcaattg agcccagcat taattgtaca
     9181 cgagttttct tcactaacgg atagtatcag gatatactaa tactatcctg aatagcgaaa
     9241 tgaggtagcc aaaatgagaa aagtcgatga atatatcaaa aagaaatccc aaaaaagtaa
     9301 agtatggcaa agaacatttg agttagagta taagaaactc tcagatagcc tcaaaaagga
     9361 cacttataat ccaaatgaac aaagtaaagc agataaccaa taaagattac cctcccatat
     9421 ttaacattgc ttcacgaatc agtaatcaat gactacaagt agtcattgaa gcaaatcttc
     9481 ataagatacc tgtagaatat ccctaatagc tctcaattga tccagcttaa tatgttgccg
     9541 gccaccttca atcttaacca atgtttctct tgtcatagcg attttactga gctggagttc
     9601 tcgcaccagt tctgtctgtc ccattccctt ttctaatcgg attcgtctga tattgctacc
     9661 gattacattt tctttatcta ttaacttttc ttccttcaaa aatagcacgc cttttggact
     9721 aaaattagtc ctttttctta ttaatcttaa agatttggta tgctacaatg ggactagtat
     9781 tagtccatat tcaattttaa ggaggaattt ttttgaagaa atttgcaatc attctaacct
     9841 gtgctttact cacgggagct ataagtgggt gcgccacttc tgacacaaat tctacaagta
     9901 ctgaaacaag tacaacaacc tcagaaaaga attatcctat acattttttt ccagttccag
     9961 atggatttga agcttattat catgataaaa aactagatgt tccattgtca gaaagtaact
    10021 ctattgaaaa agttttgcca attgaaattg aaaaaaatca agagaatata aaactcaagg
    10081 ccgagcttcc catactgaac agcacagaaa ttgattggca cgagaatatt aaagtaacta
    10141 ttgaaaaagg agcgttagca caactttata ctgaaaaagt tgaacaattt tttgagtctg
    10201 gtgccaataa agacttttca aaaataagcg attattcgga taactttttt aatgagaccc
    10261 aagatgaaat cacgaactca acttttatgg ctgaagatat gtacaatatg acgttaaaaa
    10321 aaataaaagc ttacgaccat ccattaaatc tgacactaaa taatggatta ctctcttttg
    10381 tattagcagg ttacgttgac tatgaatatt caccagatta tttggagaca cctcttcaac
    10441 acgagaacaa tgatattaat tttagatttg tctacgagtc aaaaagtaaa acatggttag
    10501 ttgatcaatt aagcactata aacacaagct tccatgaatt acctaaacct gaaaatgaag
    10561 ctgatatggt cattaaaatc ttctaaaaaa gaagcgttga aggagaaata ttcctttcaa
    10621 cgctcttttt tatatgctta gaaacataaa taaattgtat actttctttt tagcccctat
    10681 tacttttcac gaaaaatatc ttttttcgac actagcgttt ctctacttat aatattgtac
    10741 acagtttttg tattattgaa aaattactca ttatttagca ttaatccaca tcgttttatc
    10801 aatcgacctt catactaaac tactttaaag tcatcttcct tctcattttt cattaaatca
    10861 gatacataag cccatgtacc cgttcgttgt aaataaagtt cttcttgtag cagctcccac
    10921 tttctcttag aaatcataat gacgccatcg ttcaatccat ttttagaatc cctagtaatt
    10981 tctatttcag aatttgaatg aactacttct tgcaacactt tacctatatt ttttttgaag
    11041 tttgttggag taatagtatc caaaatgaac acctctcttc tctatacctc tattatgaag
    11101 cgcccatcaa ataaagcaag cgttgaggaa taattctttc aacgcttgct ttattaagct
    11161 ttcattttgt agtttgacta ataccaataa tctgatctct aaacaatcga aaaacttctt
    11221 gtggttgata ataatctgta tctatttcaa atgtttctga gaacaattca ttgaattttt
    11281 ctactgaaat gatgatttta ccattctcct ctcgactgtt ttctattatt tctgcttcta
    11341 attcaaataa atctagtgac gaactaaaat cataaatcct gtcatattga acaaaaaaat
    11401 caagaaaaag ttgctcaaac tcttccttat ttatcactag tgtttcaagc ggaacaactt
    11461 tattttcatc agccattctt ccaatttcct cactttcatt ttcaaaaaat cgtaaaattg
    11521 atatagattc tttgtctcta atcactcgtg atattatctt gacagtccta catctaattc
    11581 aattaaagta taagccactt tcaaataaaa gattacaaag taaatggcaa gccaacaaaa
    11641 taaccatctg cttcacgtat tacgatttga taattctgga tttcgctcgg aagttcataa
    11701 taataattaa cagtttctgc tttgccactt tcaatatatt ctggattatt caatatcata
    11761 gcatactcat aaagttcttc accattttta tttggttcta taatattttg tgttggtgga
    11821 aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa agagactcac
    11881 ttttggtaaa atttagtcga ccaaaacgtc atcctaccaa aggagtctct caatggataa
    11941 tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg aagaatggct
    12001 gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaaggaaaat tggatgactc
    12061 tccttcctat tgtgaacact gtcgatcggt tcgtattatc cgcaacggtt cctatacgac
    12121 acgcactcag atgttgaaag tcaaagaaaa gttaactatt ttagaattaa aacgtacccg
    12181 ttttctctgc catgattgtg gaaggacctt ttctgcgaaa acggacttag ttgatgaaca
    12241 tcatcaacta acaaaggaac tgaagcaggc aattctcatg gaactgtacg aaaatcaatc
    12301 acgtaaacta atcgctaaga agtacttcgt ctcggatggg acggtgacac ggatcttgcg
    12361 tgaagcaacg aagcattacc agccacgtat gaacttcctt ccgacggtgt tgtgtatgga
    12421 tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt tttatttgtg ttgatgggac
    12481 aaccaatcag ctatttacga tccttgaaga tcgcagactt tacaaactaa ctcaatactt
    12541 cctgcacttt cctagaaaag cccgtttaaa ggtcaagtac ctagtgatgg acatgaatgc
    12601 cagctattgt cagttgctta aaacagtctt tccaaatgcg gagatcgtaa ctgaccgctt
    12661 tcatattgtc caacacatca atcgcagctt caatcaatta cgtgtccaga tcatgaatcg
    12721 ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa
    12781 actcttactc aaagattcca cgacattgga gccactcaaa cggcattatc atcgactgtt
    12841 taaacgacca attagtcaaa cagaaatcgt tgatgagctg ttaagctaca acgaagaatt
    12901 gcgaactgcg tatcattttt gtcaattatt acgctattat ttcgttaaac gaggtacgga
    12961 aggctttcaa gaaaccctta aaacaatttc atctacgtta cctcagtcgt ttaaaaagaa
    13021 attcacgatc ttccatcgct accaatcggg tatttcaaat gcctttaagg tgtcccactc
    13081 aaacggcgtc actgaggggc tgaataacaa gatcaagctg attaaacgaa ttgcgtttgg
    13141 ttatcgcaac ttttataact ttcgggcacg gatctattta caacaaggtc tgatctttga
    13201 aaactaaaaa atatgcggcg cacagaacgc gccgcatatc ggactaagtt ttattcaaat
    13261 ttgtcttcac caacacgatt tgacgaagag ccttttattt tcaatcgacc attgttgcgg
    13321 acgtatcgga ttttctgtat catttttcat tgtataggtc acttgtaata cacgatcatg
    13381 ttgcgcaaat tcatttcttt tttcggtcaa ttcaaccttt ttaaacgtta tttcgacccc
    13441 atctgcctga taagtttcac ccatttttaa atctttagtt acaattttta ctggtttttc
    13501 ttctggaaaa tatctggctt taattgttag atagcttcca aaaagaatca atcctagtag
    13561 caataaaaga attcctttaa agagccactg aaacggaaaa tacactttaa acatctttcc
    13621 cctcctccgt tttgcatttg ttttttagat aatacgataa cttttgatca atttcatcca
    13681 actttcccac cacaattgat tctatttcag aaattttttg ttcaattcct ttgctttcag
    13741 gattacttaa ttttacattt gattggttag ctaacactgg taaatttgat accatcacac
    13801 gaaacatatt ggcttttgtt ccgaaaatcc ctttgttctg ttcgtaaaaa ctgtcaaaaa
    13861 ttgttttagt ttcttttgaa caatacactt taatatacgg atcagacatg tgtttttact
    13921 ccttatcttt ttttatttag ggagtctgta gggagcttgc tccctcagag gttttcccca
    13981 tctaacttgc cagaaagtag ccctgcgtaa gcaagggcgg attgactggc aagtagatgg
    14041 gtagctggcg tatgccctca aaaccgcctc tttccaattc cctccgtatc gatgggaaac
    14101 tggaaagtct actggctaat tcctttagaa atccatctca aaatctcgtt gtctcctttg
    14161 ctgattttca atcatttgtt gcgtgaactc gtaatctttt ttagcacgat acattttcac
    14221 atcgtcattc attatgcgac tgatttgata gtgaatctgt ttagcagaag gtgtatgaga
    14281 acaattttta aagccctcag ctggtagaaa taattctttc tgatctgtct ttttaactaa
    14341 aattgtttta cttttagtac tacgtaaagt tctaggattc acatcagtat gaagtttaat
    14401 cgtgtattct tgatcatctt ctaaagatat tgtgtttgca taatgcctgt ctacttctcg
    14461 caagaaatca tcatatttaa tgattttttt agcaccctca tgatcactaa actgcttttg
    14521 tgcaatggaa tctaattcca caaacggtac atttaactcc ccagttttat aatcaactcc
    14581 actagctaac gctataattt gttccgtatc tagatcaaca aaattattca tatgtgtttc
    14641 ttctaaattc ttttcaagtc gttcaaaaac agattcaaat gaatattttt tccctaactt
    14701 tcttgcacta aaagtacgtt cgacatactg acttttttct tttctagatt gtttttctct
    14761 tcctaaagta attagctcat ctctacgctc atagaaattt acatcttgtt tgtaaagaac
    14821 atagccaagt ttgttatttg tttttgaaaa ttgataaact tcgatgttgt attcttttgc
    14881 taaatgatcc gaaaaacgca caaatgaatt tacttcacaa ttctccaacg tagcttcaat
    14941 cgcattttgt acaaactctt gataattaat ttttccattt ttcagtttac ttggatcgtt
    15001 tgaatagaga ttcgttctct tttctaaatt ttcatagtac tgattgtcta caggttcgag
    15061 tccattttca acaagcacct catcgttaat ttttcgcatt cgttctaaat ctttagggtc
    15121 aatacgcaac ctcttacagg tctccatatc aatattatta ataacaagat gactatgaaa
    15181 atgatcttta tcattgtgac ttgcaacaat aatttgcgaa tttggaaaag ctttttctgc
    15241 aaattcgaca gctatttcgt gcattttctc aatacttaac tctttagaaa tattcttatc
    15301 aaacgaatgg ataacatgaa atgcttgtgc tatatcttca gatttattca tcctggttct
    15361 tactaaattc atttcttgca atgcttcctc actattatca ctacaattta ttcctgagac
    15421 tgcaacgcat ttttcttttt ttgaaggatt agctgcatat tgtaatagcg aggataaatt
    15481 tttcttgcgt cctttcgatg ttatagtagc tcccaaagtt gtttcacctc atcttttaaa
    15541 cttgcaataa tttgaatcga gtgaattaaa ttccggtcaa ctttttggga atactcttct
    15601 ttcgtatcgg gtctagtttg cttttctaac caatattgaa aataatcttt ttctacttct
    15661 gacagtttga gaaaagaaga tgtactccga ttggactcca aaaaaatgtc atctggataa
    15721 ctaatcatca ttttttttgc tatgctattc gcaagttcat ctttaaaaat gttgtaacgg
    15781 ctaacgtcaa aactaaaatg caggctcttt tctttcttat tttcagattg ttctctaaaa
    15841 tatttagcaa tgatatttaa tgcttttacc gcttgattca aattgttacc aatttgattt
    15901 aactgtttga ttagagctac cctatcatct tcagccaata acggcggttc atatttgagt
    15961 ccctttaacc gctcacgaca aacatctgct ctagaaattc ctctttgttt tgcaatcaat
    16021 gtcagttcaa gataagtctt tttatcgaca cgaaaattta aggcctcact atgtttgcga
    16081 tttttttcgt acattttttg tttatcatcc attgattatc ccctcacatt ttttctagag
    16141 agcaacattt gaaattgctc tctatttcat ttttcatcat taattcttct tgaatcatca
    16201 gttcaatata atttgtgacc gtgatttttt catttgtcgc tttctttact gagtcgcata
    16261 attcggcaag ttctaagaaa tcctgcgtac taatctgtag aattaattgc atgacaatca
    16321 cctctttatt ttttaaatag actatcaaac agcggatttg gtttatcaga aatatcctga
    16381 aacagtggat tattttcata agtaaatcgt ggacgtttct tctccttcac ttcttgttct
    16441 acaatcactt tttcgttagg cctttctttt cgttcctcga taatatttat tttttgcgtc
    16501 ttatctccct gacttgcttc agctcttgtt tcacgtactt ctaccgttct tggttgattt
    16561 gacgcactac tttgtacatt ttctcgtaca tctactgttc gatttccatt ttgactactt
    16621 gtagtattct gcaagttttc tcgtacatct accgttctcg gttgacttgt cccactactt
    16681 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
    16741 aagttttctc gtacatctac cgttctcggt tggcttgtcc cactactttg tacattttct
    16801 cgtacatata ctgttcgatt tccattttga ctacttgtag tattttgtaa gttttctcga
    16861 acatctactg agcgcctatc ttgactactt gtagaatttt gtagattttc tttgactgat
    16921 ttttctaatc cggtatttga atttttagct tcttggattc tttctttagt caacgcttct
    16981 ttttcgtcca tattagcgcc tgtacgccct tctacacgtt tccttaacat ttcctcacga
    17041 ttcattgctt caggcgtgta ttttgcttcc tgtcgcttct gattaattaa tacttcagca
    17101 gacttagctt gaccgtgcat agcattttta cgttcttcaa tatcttgttt aactttttgc
    17161 tgaattgcac ttgcaggatt aatatttgat tcagcattat ttttcattgc ttcacgcata
    17221 cgttcagaac tagacataat tctatcagcg ttttcaactt tagcgccatc ttttctatcc
    17281 gaaagacgtg ttggcataga atcagcatat tttccagttt catcttcttt gtttttagct
    17341 actataggtt tgctattttt attaatagcg tacacggtac catcatctaa accttctttg
    17401 aatttattct tggtatcatt agccatatca cgcatactct taaatggttt tgttgctctt
    17461 tcactggcca ttgtagctcc atccgaagct aagcctgata atcctcgttc acgagcatac
    17521 ccaagtgttt tccccgtttt attagctttt tgagaaagtg attgaccaaa gttgcgttca
    17581 ggttttcgtt cttccccttc cgcttgatcg tttccaccac tatttttccc acctttagat
    17641 cggttcatac cacctgttcc tttacgaaac agcatagctc ccatgccaaa tgctgaagca
    17701 agctggccat agccttcttt taaaccaata tccacgccaa agtaacgcat gactgttcca
    17761 ctacccttta tcagcacaat aacagcgcag acgtacgcta ctgcttttat aaaaggattt
    17821 acagaagaac cgctacctgt tccgagataa ttaacaaaca ttgcaaacat agacaatccg
    17881 aacccttgaa atccgactgt caaataacac tgtaaaatat ctgacaaaac ttgctttgat
    17941 cgttggcctg tttccaagtc agtagcaaat actaaaactc caagtacgcg tttaaacgct
    18001 agctcgagta ttgctgtaat tataatgaaa gcactaaaaa catacgctac cgctaaagca
    18061 atcaatccaa aagaaatccc ccatagattt gcttgatagc gataatatcc tgatttaatg
    18121 ccctcaacaa aggtcgacag aaaattatcc gaaaatttgg tagcaacaaa ttcattatca
    18181 gaattttcaa ctaattcata acgcagatta tcagctattg ggttctcttt ttccattttg
    18241 tcgagtacat tatctgtcaa tacttgcgta agatccgttt tccaaaaatt ttcttttgtt
    18301 agattgttcc ggtcattcgt tttatcgatc atggaatatt gatctgtttt attgatataa
    18361 gctaaatcag taaccccttg ttcaatcagc gaccacgcca cgccttcgtc cgaatttgta
    18421 atcgtagtcg tatcattata aaatgtttta gaaaactcta ttccgctgtg aatcaacgtt
    18481 ggcattaaaa gaatcaatga aattgacatt acaacattca ttccaacaga ttttagatca
    18541 accgttccct tacctgtaat cattttataa ccaataatca ctagtgaaag tatcattaag
    18601 ccaacaacaa tggtacttaa aacagcttga tagactttgt tcaatccggt actttctaca
    18661 aagctaaaca aatctaaaac ttcgggaatc atattttgaa tgccatcgac aacagcgaac
    18721 attgatttta taacagacca gcctatccac ataccgaact ggctgtaaaa atttccgttg
    18781 tattttttca aataattctg gtaaagcgga ttagttaaaa actcagtttg actctcagta
    18841 tcatcaaacc aattgaaaat actgccttct gcaatgttac tcgtaaaaag aatcacagct
    18901 attagaacaa gaaaaataac cggtaaccaa tttgtttttc tattagcaat ctttttttta
    18961 attattttca ttgttacctc cctcagccca ttttcttagg ctctcagaga gtttctgagc
    19021 tatttttctg tcaattatga tatgagttac gtaatcatca ttaacagctt ttaaatcgag
    19081 aacaatagta tttgagcaga tactctcggt taactcaaga gtatctgcgt tttgatcaag
    19141 acagtgaatc ttatctacta gatgattttc aatcatttta aattccctct tttttttgta
    19201 gcttgattag ttctaacgct ttgtttttta gagaagtaaa gatataatta tttttttgtt
    19261 tgatacgatg atctgttttt gctcttcgta tcaagtcacg taaagtcatt gctagctgtt
    19321 ctctaacatg atctacaaaa taactattat tacaaatttc atagataatt ttcagttgaa
    19381 ctttttgttc tttttcagca gatttaattg cataattgaa ttttttgtac cagttgtcga
    19441 attgacgata atcactattg ctagccagtt ttaagcaatt cacaatactt tctccccaca
    19501 tttgtttaag aagattttct tcaataaatg tttcgatcaa ttcttcatct aatttattat
    19561 ttttagttga aagtgattca aaaagatcat cattatgtga ttctttaata tctttactat
    19621 ctttataaga cttatataat tctagttcat tttttgaact atcagcaaaa ttgtttttgt
    19681 ctcttgtttt cattgatgtg tcgcatagtt caaattttga actatcaaca gaatattgat
    19741 ttgttccata gttcaaattt tgaactatca agtttttttg actattttct tgatgggtta
    19801 tcttattaat tttataaaca tcatctttgc acagtacagg ctttaacaaa taataaaaat
    19861 ttgggaacat ttgcatttta tttttattga agccattttt tcttttcaca agaagtttaa
    19921 ctttttgaag ttcatctagt gctgataaga ttgtattttt tgacattccc gaaatttcta
    19981 cgagattttt gatttcaaat atcaaaaaaa ttttttgatt ctcatcatac aagttatttt
    20041 tcaatgaaag tctatttcta tcttttagta acatataaac taacttagaa cttagtttca
    20101 ttttcttata tttttcattt acgtttaaaa aaataggata ttgatagtat aattctgcat
    20161 atatatcaga attagaatag ccttgctttt caactaagct ttcaacttct ttttcattca
    20221 ttgtcacacc tgcttctgat tttatttgct ataatagatt caaataaagt tttctcagaa
    20281 ctttattatt gagtgttccc ccgatctgca aactgacggg aacactcttt ttaatttttt
    20341 ctacttcatc attattcaaa aaaacacctc gttttttaaa tttgatgact caatatccat
    20401 tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc
    20461 attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt
    20521 ccttttagaa atggtctttc taacccggta tcaattcttg cttgatcaat agcctctttt
    20581 gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta
    20641 attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc
    20701 aagttgtatt tatttagatt tcaaatcatt aagtaaccaa tttaccaact tttgataagt
    20761 agatgctcta atactttctt tgctcccatt ttcgattttg acatatgttt tccgatttat
    20821 acctatcaat tttgctgcat cgtttttagt aagtataaga tcggctctat gtcttcttaa
    20881 gcttctaatg aattgatcat taagagttat cattcagcgc ctccttttaa attattcact
    20941 ttgtggtttt tatcttaata agcacatttt aaaccacgaa acgagtaatg tcaattaaaa
    21001 cattactcaa tttgtggttt ttatgctata atagtttgga atttactata gggggtgtta
    21061 ctttgaatag aataaaattt tatattgatt ccagtaaaaa gaccattaaa caaattagta
    21121 cagaaactgg tattaactat acaactctta cgaactatta ccgtggtgag cgaaatccta
    21181 gaaatactga aacatggaat atccttgctg attatttcca tgttcctgta agttatttga
    21241 tgggattaga cgaaacaaat tccatagaac aacctaatct tgatagaata aaaactgctc
    21301 gtttagaaaa aaaggacaaa atagtcaaga atattgccag tcttgactca gattatttta
    21361 ctgaattatc taatctatac ttttccatga cttctttact tgaagtcaaa cttcaagaag
    21421 atgactccga ctttcttttt ttaaaaagta ttctagagga ctcctttgaa tacacgattc
    21481 tatttgccaa acaaaagaag tttattaata aagctttaaa cttaaatatc gatgaaagaa
    21541 aagagttaga atctgagtta tttggcaact taacccaagt acaggttaac aatctaaaaa
    21601 gagagataac agctcgatta atatcaaaac ttcaataaaa ctaattattc aatcagccaa
    21661 ctcgccaagt tatcagattg gagagattag ttatgtcaat acaaaagtac acaacaaaaa
    21721 gcggtgcacg atatagtgtc cgcttgtatg ttcgtaaaga tgaaaatgga aagcaagaat
    21781 actatgtaaa aaaagggttt aaaactgaaa aagaagccaa gttacacgaa gcaagaaaaa
    21841 aggtcgagat tgaagatacc ggctatcata agccttctaa tgacttgttt ataacagtgt
    21901 atgaagaatg gttgccttct tataggaatc gtgtacaaga aacgacatat caacgtacaa
    21961 aagacctttt ccgcctgcat atactaccaa aattcgggaa taagtccatt tctaagatta
    22021 ctcctgttca ctgtcagaat gcagtcaatg aatgggcttt aaaattcaag aatatcaaac
    22081 agcttaagtc ttacagctct caaatatttg agtatgccat ttttgcagag ctattacacc
    22141 gaaatcctat ggcaaataca aatctaccaa agcgggaaaa gaaagaaaat gaaaattact
    22201 tttcattaga agaactaaaa gctttttggg atattctcaa taaagaagag cctttaaaac
    22261 atattttaat cttccagctt ttgattacta ctggtatacg taaaggagag ctagctgctc
    22321 ttcattggtc tgacatttat tttgaagaac agctaatgta tgttagaaag agctatgcaa
    22381 ctattcgaaa tgaagagaag aatgcaagaa gaaaaactgt caggattcaa aaaaatacaa
    22441 aaaatatatc ttccgaacga atattaccaa ttgatgcaca aactattgaa atgttaaaaa
    22501 tctggaaaaa agaacaagct cgagagcttt tgcagtttgg cataaataca aatacaaaaa
    22561 atcaacttat ctttacctat gtaaatgcag acggtgacat taatcaacct ttgcatgcgg
    22621 actattcaaa taatatattg aaacgtttag agaagaaata taaatttaag cacgttacta
    22681 ttcatggatt ccggcacact cacgctactc ttctactaga aggtggagca agtatcaaag
    22741 aaacacaaga tcgattgggt cataaaaatg ctgaaactac ccttaacact tattctcatg
    22801 ttactgaaaa agctcaacga aatgcgttag aaaaattcat tgcttacact ggtttataat
    22861 tttgtcatta cctttctcat taccagaacg aaaaaaagac accttctcgc aatgagaaag
    22921 tgtctgaaat cgttggtata acaacgatta acgttttgaa aactgtgaag ctttacgtgc
    22981 tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc
    23041 acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg
    23101 acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata
    23161 tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg
    23221 tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag
    23281 acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa
    23341 tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc
    23401 atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt
    23461 gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg
    23521 caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat
    23581 tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa
    23641 atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc
    23701 aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg
    23761 ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag
    23821 ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat
    23881 ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt
    23941 atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga
    24001 aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg
    24061 ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt
    24121 ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg
    24181 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
    24241 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc
    24301 aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc
    24361 atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg
    24421 tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag
    24481 cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca
    24541 gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg
    24601 atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc
    24661 attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca
    24721 acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat
    24781 ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga
    24841 aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag
    24901 ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct
    24961 gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat
    25021 cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac
    25081 tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg
    25141 tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg
    25201 gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc
    25261 cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt
    25321 tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg
    25381 gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa
    25441 aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa
    25501 cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg
    25561 ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat
    25621 ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg
    25681 cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca
    25741 aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg
    25801 ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt
    25861 caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg
    25921 ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga
    25981 caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt
    26041 tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca
    26101 cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc
    26161 gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg
    26221 agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc
    26281 agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca
    26341 aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt
    26401 tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt
    26461 tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg
    26521 gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc
    26581 agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc
    26641 gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct
    26701 gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg
    26761 gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga
    26821 tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca
    26881 cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat
    26941 ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct
    27001 atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag
    27061 agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact
    27121 ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt
    27181 tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac
    27241 ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca
    27301 caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg
    27361 ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga
    27421 cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat
    27481 ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc
    27541 ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt
    27601 ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag
    27661 aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga
    27721 tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg
    27781 tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag
    27841 tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc
    27901 aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat
    27961 cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta
    28021 ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt
    28081 taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac
    28141 atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca
    28201 tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt
    28261 tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt
    28321 ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt
    28381 tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg
    28441 aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga
    28501 gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat
    28561 tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac
    28621 cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag
    28681 tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg
    28741 atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc
    28801 acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt
    28861 cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca
    28921 atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt
    28981 acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg
    29041 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    29101 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    29161 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    29221 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    29281 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    29341 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    29401 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    29461 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    29521 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    29581 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    29641 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    29701 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    29761 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    29821 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    29881 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    29941 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    30001 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    30061 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    30121 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    30181 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    30241 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    30301 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    30361 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    30421 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    30481 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    30541 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    30601 tattgtcgat atattctgtg tattcatctg gagcaatgac tctgtgtatc tctaaatgat
    30661 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    30721 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    30781 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    30841 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    30901 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    30961 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    31021 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    31081 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    31141 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    31201 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    31261 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    31321 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    31381 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    31441 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    31501 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    31561 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    31621 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    31681 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    31741 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    31801 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    31861 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    31921 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    31981 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    32041 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    32101 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    32161 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    32221 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    32281 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    32341 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    32401 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    32461 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    32521 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    32581 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    32641 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    32701 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    32761 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac
    32821 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    32881 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    32941 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    33001 tccagcgcct atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta
    33061 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    33121 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    33181 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    33241 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    33301 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    33361 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    33421 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    33481 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    33541 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    33601 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    33661 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    33721 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    33781 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    33841 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    33901 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    33961 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    34021 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    34081 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    34141 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    34201 cataagaaat gatcccgcca tagccgatcc ctattaaaga agcttgtaat gcggcagcac
    34261 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    34321 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtgtttt tctgctaatt
    34381 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    34441 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    34501 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    34561 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    34621 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    34681 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    34741 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    34801 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    34861 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    34921 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    34981 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    35041 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    35101 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    35161 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    35221 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    35281 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    35341 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    35401 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    35461 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    35521 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    35581 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    35641 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    35701 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    35761 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    35821 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    35881 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    35941 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    36001 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    36061 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    36121 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    36181 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    36241 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    36301 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata
    36361 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    36421 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    36481 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    36541 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    36601 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    36661 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    36721 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    36781 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    36841 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    36901 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    36961 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    37021 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    37081 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    37141 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    37201 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    37261 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct gtcatcgtgt
    37321 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    37381 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    37441 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    37501 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    37561 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    37621 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    37681 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    37741 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    37801 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    37861 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    37921 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    37981 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    38041 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    38101 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    38161 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    38221 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    38281 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    38341 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    38401 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    38461 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    38521 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    38581 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    38641 ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    38701 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    38761 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    38821 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    38881 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    38941 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    39001 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    39061 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    39121 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    39181 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    39241 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    39301 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    39361 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    39421 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    39481 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    39541 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    39601 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    39661 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    39721 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    39781 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    39841 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    39901 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    39961 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    40021 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    40081 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    40141 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    40201 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    40261 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    40321 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    40381 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    40441 gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    40501 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    40561 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    40621 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    40681 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    40741 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    40801 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    40861 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    40921 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    40981 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    41041 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    41101 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    41161 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    41221 gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa
    41281 acacatttac ccttatttca atccatgtta tttggggata cttatctcta aaacataaat
    41341 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
    41401 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
    41461 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
    41521 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
    41581 ttagacaaac caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat
    41641 ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat
    41701 actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg
    41761 cataccagta ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag
    41821 aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga
    41881 tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg
    41941 tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc
    42001 tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac
    42061 tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg
    42121 catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg
    42181 aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat
    42241 catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc
    42301 tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat
    42361 gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat
    42421 ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa
    42481 tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac
    42541 tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca
    42601 gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc
    42661 atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc
    42721 atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa
    42781 tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc
    42841 gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta
    42901 acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga
    42961 gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat
    43021 gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga
    43081 aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc
    43141 ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc
    43201 ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg
    43261 tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc
    43321 tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat
    43381 cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt
    43441 tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc
    43501 agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt
    43561 cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat
    43621 catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac
    43681 accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg
    43741 atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt
    43801 acctagaacc atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat
    43861 gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac
    43921 gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg
    43981 caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg
    44041 aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc
    44101 ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag
    44161 ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt
    44221 tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga
    44281 gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat
    44341 atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc
    44401 gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat
    44461 tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg
    44521 gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg
    44581 taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga
    44641 agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg
    44701 tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag
    44761 taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg
    44821 tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg
    44881 ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc
    44941 taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc
    45001 tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc
    45061 gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc
    45121 ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa
    45181 ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt
    45241 ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg
    45301 ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc
    45361 tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc
    45421 tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc
    45481 ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg
    45541 aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa
    45601 ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt
    45661 gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat
    45721 ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa
    45781 acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg
    45841 tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat
    45901 cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt
    45961 tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc
    46021 accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg
    46081 taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat
    46141 atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac
    46201 aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg
    46261 aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg
    46321 agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt
    46381 atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga
    46441 taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc
    46501 tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt
    46561 agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata
    46621 ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat
    46681 cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag
    46741 agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt
    46801 ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa
    46861 ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc
    46921 atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc
    46981 tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc
    47041 ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg
    47101 aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt
    47161 atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa
    47221 tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc
    47281 agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata
    47341 aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa
    47401 cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg
    47461 aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga
    47521 aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa
    47581 ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg
    47641 ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat
    47701 acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg
    47761 atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata
    47821 gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc
    47881 gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg
    47941 gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag
    48001 gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt
    48061 ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa
    48121 tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc
    48181 gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt
    48241 tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc
    48301 gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa
    48361 gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg
    48421 aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc
    48481 tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc
    48541 gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc
    48601 tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac
    48661 ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg
    48721 tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa
    48781 ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc
    48841 ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc
    48901 gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg
    48961 ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa
    49021 aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga
    49081 cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta
    49141 cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt
    49201 ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa
    49261 agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt
    49321 ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt
    49381 tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa
    49441 cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat catgatggat
    49501 acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa
    49561 agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag
    49621 cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat
    49681 ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg
    49741 attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga
    49801 ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga
    49861 tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa
    49921 tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga
    49981 gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc
    50041 tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca
    50101 agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat
    50161 ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat
    50221 cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa
    50281 agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt
    50341 ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa
    50401 cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt
    50461 cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta
    50521 cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc
    50581 aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc
    50641 caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt
    50701 ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc
    50761 ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct
    50821 gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat
    50881 ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga
    50941 ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt
    51001 ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca
    51061 acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc
    51121 ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca
    51181 ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac
    51241 acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc
    51301 tccttacttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta
    51361 attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc
    51421 cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc
    51481 gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt
    51541 ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt
    51601 gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga
    51661 tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga
    51721 cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat
    51781 tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg
    51841 tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc
    51901 gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct
    51961 tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt
    52021 tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag
    52081 ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca
    52141 ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca
    52201 ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg
    52261 ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa
    52321 aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat
    52381 taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa
    52441 cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt
    52501 gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc
    52561 ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc
    52621 tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca
    52681 tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc
    52741 tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg
    52801 atttgaaagt acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag
    52861 cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg
    52921 gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc
    52981 tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct
    53041 gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata
    53101 tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa
    53161 gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc
    53221 attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg
    53281 tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc
    53341 cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt
    53401 tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac
    53461 tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat
    53521 ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc
    53581 ttttttctga attctcttca aaagactctc cttattctat cagctgacag tttctctatc
    53641 ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat
    53701 caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc
    53761 ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact
    53821 tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct
    53881 cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat
    53941 tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc
    54001 cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc
    54061 ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc
    54121 gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga
    54181 agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca
    54241 agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat
    54301 agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag
    54361 catatctaaa taatccattt attttcctcc tttttattta tcctacttac tttttcctaa
    54421 ttctaggata cctacttata tgttactaca cacccttatt ttttacactt taacagtcac
    54481 catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag
    54541 aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa
    54601 cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg
    54661 aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc
    54721 gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa
    54781 tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa
    54841 aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc
    54901 aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat
    54961 acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa
    55021 taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa
    55081 tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga
    55141 atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac
    55201 gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat
    55261 cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt
    55321 aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt
    55381 catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa
    55441 tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac
    55501 tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg
    55561 cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac
    55621 caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac
    55681 aagcgacagt acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DASHWH010000011        53822 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.011, whole genome shotgun sequence.
ACCESSION   DASHWH010000011 DASHWH010000000
VERSION     DASHWH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.011"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(437..600)
                     /locus_tag="U2R41_000668"
                     /pseudo
     CDS             complement(437..600)
                     /locus_tag="U2R41_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(953..1642)
                     /locus_tag="U2R41_000669"
     CDS             complement(953..1642)
                     /locus_tag="U2R41_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM8888101.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="U2R41_000670"
     CDS             complement(1705..2469)
                     /locus_tag="U2R41_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEM8888102.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="U2R41_000671"
     CDS             complement(2497..3195)
                     /locus_tag="U2R41_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEM8888103.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="U2R41_000672"
     CDS             complement(3207..3986)
                     /locus_tag="U2R41_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEM8888104.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="U2R41_000673"
     CDS             complement(4002..4946)
                     /locus_tag="U2R41_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8888105.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="U2R41_000674"
     CDS             complement(4992..5771)
                     /locus_tag="U2R41_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888106.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6156..8231)
                     /locus_tag="U2R41_000675"
     CDS             complement(6156..8231)
                     /locus_tag="U2R41_000675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEM8888107.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="U2R41_000676"
     CDS             complement(8233..9150)
                     /gene="glyQ"
                     /locus_tag="U2R41_000676"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEM8888108.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9536..10348)
                     /gene="recO"
                     /locus_tag="U2R41_000677"
     CDS             complement(9536..10348)
                     /gene="recO"
                     /locus_tag="U2R41_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEM8888109.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10491..11390)
                     /gene="era"
                     /locus_tag="U2R41_000678"
     CDS             complement(10491..11390)
                     /gene="era"
                     /locus_tag="U2R41_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEM8888110.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11405..11800)
                     /locus_tag="U2R41_000679"
     CDS             complement(11405..11800)
                     /locus_tag="U2R41_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEM8888111.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="U2R41_000680"
     CDS             complement(11784..12260)
                     /gene="ybeY"
                     /locus_tag="U2R41_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEM8888112.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12289..14481)
                     /locus_tag="U2R41_000681"
     CDS             complement(12289..14481)
                     /locus_tag="U2R41_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEM8888113.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14505..15476)
                     /locus_tag="U2R41_000682"
     CDS             complement(14505..15476)
                     /locus_tag="U2R41_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEM8888114.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16182..17561
                     /gene="brnQ"
                     /locus_tag="U2R41_000683"
     CDS             16182..17561
                     /gene="brnQ"
                     /locus_tag="U2R41_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM8888115.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17717..18163)
                     /locus_tag="U2R41_000684"
     CDS             complement(17717..18163)
                     /locus_tag="U2R41_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEM8888116.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18190..18366)
                     /locus_tag="U2R41_000685"
     CDS             complement(18190..18366)
                     /locus_tag="U2R41_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEM8888117.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18528..18956)
                     /locus_tag="U2R41_000686"
     CDS             complement(18528..18956)
                     /locus_tag="U2R41_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8888118.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19048..19908
                     /locus_tag="U2R41_000687"
     CDS             19048..19908
                     /locus_tag="U2R41_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEM8888119.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20003..20353)
                     /locus_tag="U2R41_000688"
     CDS             complement(20003..20353)
                     /locus_tag="U2R41_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM8888120.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(20369..22096)
                     /locus_tag="U2R41_000689"
     CDS             complement(20369..22096)
                     /locus_tag="U2R41_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEM8888121.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="U2R41_000690"
     CDS             complement(22310..23236)
                     /gene="trxB"
                     /locus_tag="U2R41_000690"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEM8888122.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23381..24817)
                     /locus_tag="U2R41_000691"
     CDS             complement(23381..24817)
                     /locus_tag="U2R41_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM8888123.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24814..26217)
                     /locus_tag="U2R41_000692"
     CDS             complement(24814..26217)
                     /locus_tag="U2R41_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM8888124.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26447..27349
                     /locus_tag="U2R41_000693"
     CDS             26447..27349
                     /locus_tag="U2R41_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8888125.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27390..29276)
                     /locus_tag="U2R41_000694"
     CDS             complement(27390..29276)
                     /locus_tag="U2R41_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8888126.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29372..30223)
                     /locus_tag="U2R41_000695"
     CDS             complement(29372..30223)
                     /locus_tag="U2R41_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8888127.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30680..32464)
                     /locus_tag="U2R41_000696"
     CDS             complement(30680..32464)
                     /locus_tag="U2R41_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888128.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEKYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32464..34215)
                     /locus_tag="U2R41_000697"
     CDS             complement(32464..34215)
                     /locus_tag="U2R41_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888129.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(34357..34581)
                     /locus_tag="U2R41_000698"
     CDS             complement(34357..34581)
                     /locus_tag="U2R41_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEM8888130.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34832..36418
                     /locus_tag="U2R41_000699"
     CDS             34832..36418
                     /locus_tag="U2R41_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEM8888131.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(36728..38503)
                     /locus_tag="U2R41_000700"
     CDS             complement(36728..38503)
                     /locus_tag="U2R41_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEM8888132.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(38714..39616)
                     /locus_tag="U2R41_000701"
     CDS             complement(38714..39616)
                     /locus_tag="U2R41_000701"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEM8888133.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            39895..42240
                     /locus_tag="U2R41_000702"
     CDS             39895..42240
                     /locus_tag="U2R41_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM8888134.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(42403..42669)
                     /locus_tag="U2R41_000703"
     CDS             complement(42403..42669)
                     /locus_tag="U2R41_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888135.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(42662..42985)
                     /locus_tag="U2R41_000704"
     CDS             complement(42662..42985)
                     /locus_tag="U2R41_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888136.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(42997..43203)
                     /locus_tag="U2R41_000705"
     CDS             complement(42997..43203)
                     /locus_tag="U2R41_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888137.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(43200..44255)
                     /locus_tag="U2R41_000706"
     CDS             complement(43200..44255)
                     /locus_tag="U2R41_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEM8888138.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(44255..44650)
                     /locus_tag="U2R41_000707"
     CDS             complement(44255..44650)
                     /locus_tag="U2R41_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888139.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(44693..44947)
                     /locus_tag="U2R41_000708"
     CDS             complement(44693..44947)
                     /locus_tag="U2R41_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888140.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(44944..45339)
                     /locus_tag="U2R41_000709"
     CDS             complement(44944..45339)
                     /locus_tag="U2R41_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888141.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="U2R41_000710"
     CDS             complement(45693..46694)
                     /gene="ccpA"
                     /locus_tag="U2R41_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEM8888142.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            46904..48007
                     /locus_tag="U2R41_000711"
     CDS             46904..48007
                     /locus_tag="U2R41_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM8888143.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(48069..48671)
                     /locus_tag="U2R41_000712"
     CDS             complement(48069..48671)
                     /locus_tag="U2R41_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8888144.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(48674..49114)
                     /locus_tag="U2R41_000713"
     CDS             complement(48674..49114)
                     /locus_tag="U2R41_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEM8888145.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(49240..50178)
                     /gene="galU"
                     /locus_tag="U2R41_000714"
     CDS             complement(49240..50178)
                     /gene="galU"
                     /locus_tag="U2R41_000714"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM8888146.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(50193..51218)
                     /locus_tag="U2R41_000715"
     CDS             complement(50193..51218)
                     /locus_tag="U2R41_000715"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM8888147.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(51262..52092)
                     /locus_tag="U2R41_000716"
     CDS             complement(51262..52092)
                     /locus_tag="U2R41_000716"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEM8888148.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(52107..53045)
                     /locus_tag="U2R41_000717"
     CDS             complement(52107..53045)
                     /locus_tag="U2R41_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEM8888149.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(53182..>53822)
                     /locus_tag="U2R41_000718"
     CDS             complement(53182..>53822)
                     /locus_tag="U2R41_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEM8888150.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT        15005 a        11392 c         9545 g        17880 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5881 atatttgtaa acaagttttc cctatataat cataccacaa actgctttct tgactaggga
     5941 aatttacgat ttaaaagtaa ataaaacatt aataagataa caattaatcg attttacttt
     6001 tatttcctta attttcatag gtgtttttaa aagttacaca aaaaataaaa taacaaaaaa
     6061 aattaacatt gaattaataa tagttattta taacaaataa accagagaaa tagcctattt
     6121 tgtgcctgtt ctctggttta tttacatatt agaaattatt ttgtgatgat ttgatctaaa
     6181 cttgctagtg acaaagccat ggcagcaatt tgacttaatt gcgttaatcg gttcttacgt
     6241 actgctggtt cttctgccat gaccatcgtc tgatcaaaat aattttcgat cagtgggcga
     6301 aggcttacaa gctgatcata attttcgttg atcgtttggt cagcaaagct tgttttcatt
     6361 gcctctactg ctttgtgcag ttctttctcc gcttcgtttt cgaacagttc agggtctact
     6421 tctgattgtt tttcaagctc cgcttttttc gctaagttca cgacacgtgt caaagcttcc
     6481 atcgatggtt tgaagtcttc atctgcaaga tgctgcttga acaattgtgc tgctgcaaat
     6541 acttttgtca gatctttttg ttctgcattc aaaacagctt cgatgacatc atgacgaata
     6601 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ctgttgttgt
     6661 cccgctgaca agccaatgcc aaaacgatcc gcatctttac tgataacttc actaatcgtt
     6721 tcttgcagaa cagataaagg aaaggcccat tttttcgatt cgatgattcg aacgacacca
     6781 tatgcttgac ggcgcaaagc atatggatcg tttgagccac ttgggatcat cccaactgca
     6841 aagaaagaaa agatactatc taatttatca gcaatcgcta aaacagaacc tactgtagtc
     6901 tcaggtagat ctccttcact ggatataggc atgtaatgtt cacggattgc tgttgctacg
     6961 gctggtttct cgcctttaag cagagcatat ttttctccca tgatcccttg tagttcaggg
     7021 aattctccta ccatgttggt tactagatca aatttataga tttcagcagc gcgttgcaga
     7081 tccatcaatt cttcctcaga tagtcctact tgctcgccta tgatgcgggc gatgacttgt
     7141 acccgttgca ttttctcgta gatgctaccg attttttcat ggtaagtaac agtttttaat
     7201 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
     7261 tctaaacgag cagtcaatac tttttgattg cctcgaacca cattttccaa ttttacccga
     7321 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
     7381 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
     7441 ttttcttcgt agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
     7501 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
     7561 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
     7621 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
     7681 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcatc atctaacaat
     7741 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
     7801 acagggaaag tcaggctttt aatgacatct gctaaacctg tcaatacttc tttggattcc
     7861 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
     7921 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
     7981 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
     8041 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
     8101 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
     8161 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
     8221 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
     8281 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
     8341 gttgcggata cgggaaagat aaccggcacg ctctgttacg gatactgcac cgcgagcatc
     8401 taataaattg aatgtatggc tgcatttcaa aatataatca tacgctggat gaacaagatt
     8461 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
     8521 ttgatcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
     8581 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
     8641 agaagcaaga cgttctagtc cataagtgat ctcagaagtg actggtttac aaggcagtcc
     8701 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
     8761 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
     8821 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttggatatt
     8881 ctcaggtgaa ggtttcatca cgacttggaa ttgatgatgc tgatacaaac ggtttgggtt
     8941 ttcaccataa cgtccatcag ctggtcggcg agatggttct acgtaagcag cattccatgg
     9001 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
     9061 gtcatacgct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
     9121 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
     9181 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
     9241 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
     9301 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
     9361 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
     9421 cttgtttcta taatagatcc tctcactgaa cgatctattt gatctacggt taataataga
     9481 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttc atttactatt
     9541 cttctttttt aggtagatct ttttcagaca tgtctttctc tggcgtcttt aatacgtgtg
     9601 cccaagactt catttgatcg atgaactttt tgcttttcaa atgaataccg acatattcat
     9661 catacagctg gtcgatgagt tcacggattg cttgtttggt ttcttctttc agatggattc
     9721 cactgatttt atcataagaa atagctgaaa acatccgaac gaaataaaca gctcgaggat
     9781 ctgcatgata acggtgatcg tccatcgacc aatgtctttc gcatatcaca ccaccatatt
     9841 tggaagaata gtcaaatttt ccttttgttt ccccacatac gacgcagtgt gtccagattg
     9901 gtgcaattcc gaagcgttgc agtatttgaa cttcaaagat attcgtgatg atttctgcat
     9961 catctccttg atccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat
    10021 attgatgatc ttcgattgct gcatctacta gatttagcaa ataggtacca taaccgctca
    10081 aaaagatatc ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg
    10141 acaaaccttc acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg
    10201 gagctaatgg ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag
    10261 attccgtaaa gattttgact aatttatctt tttctttgta attacggcta gataaaatca
    10321 agcctttcga ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag
    10381 cagattctgc cgatccagat tatttatgtt tcgatcttcc gccgtttttc tcaacgtgcg
    10441 gatttttatc cagtttattt ctatacttaa cctataagaa gttcagaata tcagtattca
    10501 tcttttcggt agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact
    10561 ttgacccaaa gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct
    10621 ttcgttccta cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta
    10681 tctctttcga cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc
    10741 gaatcaacaa cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc
    10801 tcacggatca attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga
    10861 aaatattgtg ggccctctgg catttcactg actaatacat ccatcaaacg ttcgacgttg
    10921 tttccttctg tagcagaaat tggaatcact tgtgcaaagg gcatctgcgt tgaataatct
    10981 tcgatgattc ccaacaactc gtctggatgg attttatcga ttttattgat aatcagataa
    11041 acaggagaat gcacgttttt tagacgttca ataataaaat cgtctccccg gccgcgtttt
    11101 tgatcagcgc tgatcataaa taagaccgcg tccacttctt tcaacgcact gtaagctgct
    11161 tctaccataa aatctcctaa acgatgcttt ggtttatgga ttcctggtgt gtcaataaaa
    11221 atcaactgag cttctggtgt agtatatact ccttggattt tatttctagt tgtctgcgcc
    11281 ttatcactca tgatggcaat tttttgaccc acgattcgat tcaataatgt ggattttcct
    11341 acattcgggc ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc
    11401 ctttttaatt aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaacgatga
    11461 cggcaaaaca agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa
    11521 tcggatgaaa atgaaaatcc gtaaacatgt ctacgacatt ttcaaaaaca gtattgatga
    11581 tctctactat ccatacgaga aaaacagcta aaagcagcca taaccattct atccgatcaa
    11641 gttggaaaat cacgcccata aacaaagcga ggattccgaa cgcaacatgt ttcctcatgt
    11701 tgcgttcttc ctcaaaaaca gttttgactc cttggacggc aaattccacg gacatcagga
    11761 aatgtttgtt tttctctacg tttttatctt ttaagtccat aggcatctaa gatctctttc
    11821 tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat ggtcataccc gttgatatgc
    11881 aagaatccat gaaccgctaa aaagccaagt tcccgatcat acgaatggcc gtaagcttct
    11941 gcttgctctg cagcacgttc agtagagatc atgatgtctc ccagtacacg aggcaatgga
    12001 ttctctccat cttcaaaaat cactgggatt tcgtcttcac cctcttcttc caaggcaaaa
    12061 ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggatcgct
    12121 gcgttgtcca tgaaagtaac agacatttct gtttcttctg gcaactctaa atatgatgct
    12181 gcaaattgca acaagtcgtc gatttctttc attttctctt ctgagacttg gtttgtttca
    12241 tccatgaagg taatatccat tatacatcgc ttctttctat tcttcttgct attcttcttg
    12301 ttcttctttt atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa
    12361 ggtactgctt aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa
    12421 tccactgtca tccaattgtc catcggaaat acgctcttct ataagattat ggacaaaact
    12481 ttcgattttt tcgcttgttg gatggtccat tgcccgtact gccgcttcac aactgtcagc
    12541 tatattgacg attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc
    12601 ttccgtgact tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt
    12661 tccatgatgc tgtctgcaaa tatcgatcac catttgcggc atattataat cttccaatat
    12721 tttggcacca tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa
    12781 attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat
    12841 cttgccgata tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc
    12901 tgctacagca ttagcactta gattggctac catcatgcta tgatgatacg tacctggtgc
    12961 ttcttctaac aactgtttca ataaagggtg attaggattg ctgagttcat ttaatacgat
    13021 cacactatca tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa
    13081 tgagaacaat gcacctgtga atccgcagac tagcgttaac caagtcgttg catccccaaa
    13141 gctcattcct tggtagataa tcaaaatgac atccatcatc acagggaaaa taatgaccca
    13201 catagcagcg aataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag
    13261 accagaaaat acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata
    13321 aaaaatgaaa agtgcggata ctgcttggaa aataccagcc atgatccctg ctcttcgatt
    13381 caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc
    13441 agcttgttct gtttggaaga gttggaaaaa tttcattagt actacgctga ctgacattgc
    13501 tgtcacgtag aaaagtacgt attttgttct ttctcctgca ttttgaaatt gaagtgaatt
    13561 ataaatcagt acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat
    13621 cgaagtcgtt tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat
    13681 ttgatttcct tcgcgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc
    13741 tcgtgcagat tgttttaatt cttctgtctt tttctcattc aatacatcat tgactacaat
    13801 gccttgatcc actaagtagc ggatcatttg ttgctgctct gttgtgacat tcaaatactg
    13861 aatctgatct tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact
    13921 catctgttcg tcaatgagct tcaaactttc atccctaacc gtgtgaattt ccgtttcagt
    13981 catttcaaat atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc
    14041 tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt
    14101 cgggatattt tcatcttcct tcgccttatc taccttgtca ttataagatt tgttgatggt
    14161 atcattcgtc ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc
    14221 cagatctttt tgataagtat attctggagt cactgcttct gctgctagtt ttcttctttg
    14281 ttcggtttct tccgtgttct cgatcgtttt attcgcacgg atactctctt ctgctacttg
    14341 gccttctcta aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcaaacaat
    14401 gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt cttgtagttt
    14461 ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga
    14521 tccgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt
    14581 gcttcaaaat taacaaaatc gatttttttg atttgcttta gtgttcgttc tgcatgaaca
    14641 agaccacttg tcgtccctcg aggcaagtct atctgacttg tatccccatt gacaatcatt
    14701 tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt
    14761 gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaatggt
    14821 gcgatttcga ttacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg
    14881 tataaagcat cataaacagg ccgcagataa ggatccactt tttctttcag atcccctggt
    14941 agaaagccta ggttttcccc tgcctctaca gcgggccgcg tcaaaatgat tttttgcact
    15001 tcgccttttt ttaaggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg
    15061 gctggcccta caccaaatac gatgtcatgt ttggctaccg attcaacata tttcttttga
    15121 ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac
    15181 atctcaacaa aataatctaa tgtacctttt tgccccattt tcaatgccgt cacaacatcg
    15241 ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca
    15301 gccaaggaaa tctgggattg ttcaccaatg atctgaatga tttctccacg tgtatggatg
    15361 gttgtttccg ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg
    15421 ctgacatcat cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat
    15481 agacatcccc tttttttcga ttcattcttt cctattttac catactcaga agaaactata
    15541 tagcaaataa gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa
    15601 aaatagccgt ttccattcgc taaaggtctc gttttgataa aaagaatctg agaatgtaag
    15661 ttatatgaaa gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt
    15721 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
    15781 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
    15841 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
    15901 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
    15961 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
    16021 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat
    16081 tttaaggtgg tggttttcat ggaatctgat attaatggca ttactaattg ctgcaacgtt
    16141 tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt
    16201 attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag
    16261 gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cacaaactgg gtcagtgctg
    16321 gcgtaggatt tgccatctca ggcgctcttt tcccattact agctatctta gctgtagtag
    16381 tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct
    16441 tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg
    16501 ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta
    16561 tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac
    16621 ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcatta ttagcaatca
    16681 tttttgtcgt ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt
    16741 accaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag
    16801 ctttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata
    16861 aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga
    16921 tctattttgg acttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa
    16981 atggcggaac tgcactttct cagattacta atttttattt aggtaacttc ggcgctagct
    17041 tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt
    17101 ttgcacaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa
    17161 cgactttcgt ctcttttgtc gttgcaaatg ctggtttgga caatatcatc caatggtctt
    17221 taccagtatt gatgcttttg tacccattgt cgcttgcgtt gatcttgctt tccttaacag
    17281 caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc
    17341 ccgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag
    17401 caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga
    17461 tggtcccaac gttacttggt tacgctggca gcttactctt ctattacgcc tatcgcctat
    17521 cagggtacaa acaagaagca aatgaactac cagaagaata accagatcaa acacaattta
    17581 acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct
    17641 agctattttt tagatttctt ttttacgagc acaaaaaaac tgcggacaat gattgtccgc
    17701 agtttaaatg ataaaattaa gattgtaata gttctttcac aatggcattt acttggtttc
    17761 catctgcttt tcctttgact ttaggcatga tcgcacccat gactttgcca aattctttca
    17821 ttgatgaagc accagtttgt gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg
    17881 aaagttgttc cggcatgtat ttttcgacta tcacaatttc actttttact ttttcagcta
    17941 aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc
    18001 gagacaacac cgttaattct tcttcttctg tcaagtcgtg accttcttta atctgctcgt
    18061 tttgtataga tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca
    18121 tcgctgtttt catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc
    18181 atcacagaac tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac
    18241 acttggtttt tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac
    18301 ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt
    18361 tgacatgtta attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata
    18421 tctcaaaaaa caagactgtc ctgtaaacat tatatctaat gctgttttga ccgtcaagga
    18481 aatcttgctt ttttttaaaa ttatcaaatg ttattttatt taaatcctta ttgacaattt
    18541 tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg tacatccacc gagctgttct
    18601 tcgaaataat tcattggaca catatggatt tctttcgttg ttccgcacac cgtacagata
    18661 aagtgatggt gatggccgat ttccccttga cagcaatgaa aacgaaactt catctctcca
    18721 tttaaatcgg tttcttccag taaatcgatt tctgcaaaat catggagatt tcgataaatc
    18781 gtatcgtaac ttactccttt gaattcctga ttcatatagt caaaaacttc tttagcggat
    18841 acgtagcgat tgcgcttcac taaataagtt aacaatgctt ctcttttttt agtatattta
    18901 aaaccgttat ttttcaatgt ttctagtgat tgttcgatca atgtctgttt tttcatgact
    18961 tattcctcca aatcaaaatg attacgaagt acacattcta gagtatggta taataaacat
    19021 aaaaaaaatg caagtgaggt tttatccatg tcttccgatt ctacagaaat cgttactttt
    19081 tttgtcatct ccgactctgc tggagaaact gcgacaaaat tagcgcaagc cactatggcc
    19141 caatatccta gcgttgagtt caatttattc cgcagaacat ttgtgacaga tgagaaaaca
    19201 ttgatgcagg cactgcaaga tgcactagaa gaaaaagctc ttgtcttaca cacattgatc
    19261 aatcaagaat tgatcgagat gacgcgttct ttttgtgaag aacatcaatt atttagtttt
    19321 gatgtgatga ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg
    19381 caacctggcg cccttcattt attgaatgag aactatttca aacggattaa agcaatggaa
    19441 tttgctgtaa aatacgatga cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt
    19501 ttgctaggcg tatcccgaac ttccaaaacg cctttgagct tgtttttagc taacaaaaat
    19561 ttaaaagttg ccaatcttcc tttgatccca caagcacata ttcccaaaca gctttgggag
    19621 attgatccta aaaaaatcgt gggattgacg aataacccag atattttaaa caatatccgt
    19681 aaagaacgga tgcgctctta cggattaaat cctgatactg cttattctga tatcgaaaaa
    19741 atacgagctg aactcgattt tgcaaatgat ctttatgaaa aactgggctg cgtagtaatc
    19801 aatgtcgctt ctttatctat tgaagaaaca gcttcgctta ttttgaatgc tttagaccta
    19861 gaagatcata gttattatgg aactgaaacc accgaagaaa cagactaaca gtttttcttg
    19921 tctttttctt aaaaaaaact ggactgtgtc agcgaagaag attcgttgac acagtccagt
    19981 ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagtgtc tggttcaaaa
    20041 tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact
    20101 tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt
    20161 ccactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt
    20221 tgagacgagt ggggtcactc aacattttga atagttggct gactttctct acttttgttt
    20281 tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta
    20341 ccactttttc catcagtctc aagttttttt attcttctgt catcttttgg ataatcgctt
    20401 cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatgtaaa
    20461 atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg
    20521 tatacttcaa tacatcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg
    20581 ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgttg
    20641 gtgcttcttc acggaatttt tccatcaaag cagagatttt cgcagaacct tcttgtccgc
    20701 tcattgtcac agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa atcgtttgca
    20761 agccgtcata caacgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga
    20821 gcaatgcttg gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt
    20881 cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag
    20941 caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg
    21001 ctgtaggtaa ttcgctagaa acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc
    21061 ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt tggtttccgg
    21121 tcaatacttc ataggagcca tttggcaaac gaacggctgc acctaaacgg tctgcatcag
    21181 gatctgttgc aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa
    21241 aagcagagtg ttcttctgga tttggtgatt tgactgtagt gaaatctggg tctgcgacag
    21301 cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca
    21361 acatttttcc tgttccgtga agtggtgtgt acacaagttt caaatctttc cccattgttt
    21421 ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt
    21481 cttctccgat gatcgtgatc aatccgctat gttcagcttc ttcgtcagat aatacttcaa
    21541 cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag
    21601 gcatttgtcc cccatcttcg ccgtagactt tatatccgtt gtaagctgct ggattgtggg
    21661 atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg
    21721 gtgttggacg tagactttcg aatacataag atgggatatt gtgatgtgct aatgttttgg
    21781 ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc
    21841 ggcgttttgt ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc
    21901 ttaccgtata gatgttcatt cgattgatcc caggccctaa gattccccgc atgcctgcag
    21961 taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat
    22021 catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca
    22081 cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag
    22141 cattcactta ttagatataa aagtatgagg accaaaaaac gcttaaatca agaaaggatt
    22201 cttttatcaa gcacggttgc aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa
    22261 aaaaaagagt aaaagaacaa ttgaaaccgt tcttccactc tttttaaact tatgcagttg
    22321 tttcttctaa agattccaag tatttataca cttgttgtcc agcgatccca ccatcaccaa
    22381 cagcagtagt gatttgtcgc aaatcttttt cacgaacatc tccgatggca aaaacgcctg
    22441 gtatattcgt cttcatttcg ttgtctgttg gaatccagcc agcttcgttc gtcaaccctg
    22501 cttctctaaa tggtgcagtt aatggatcca gtccaacata gatgaatact ccgtctgctg
    22561 ccaaatcaaa ctcattgccg gttttgacat tacgtgcttt cacacctgtc actaccattt
    22621 cattcccaac gatttcgtca acgactgtat cccacataaa ggaaattttt tcattcgcaa
    22681 atgcacggtc ttggatgatc ttttgtgcac gtaattcatc tctacggtgg acgatgacta
    22741 cttcagaagc aaattgtgtc aaatagatgg cttcttctac tgctgagtct cctcctccga
    22801 tcactactag acgtttgtta cggaaaaacg caccatcaca aactgcacag taagaaacgc
    22861 cacgtccagc aaattcttcc tcgccaggta cgcctaattt acggtggata cagcctgttg
    22921 cgataatgac tgtttttcct tcgtaagttt tatcttcaca aataacttct ttgtggctgc
    22981 cgtgatcctt gatatcttgc acgattccat aagcattttc tgtcccaaat cttgaaacac
    23041 cttcatacat cttataagct agatcaggac ccatgattga atcgaaacca ggatagtttt
    23101 cgatttctgc cgtattgttc atttggccgc caggggcgcc ttgttcgatc atcaatacgg
    23161 ataagtttga acgtgaagca tataaagcag cagtcatccc agcaggacct gcgccaataa
    23221 cgattacatc atacatatgt agattctcct ttacttaatc aagtaagcat actttacagc
    23281 caatgcgaaa aaaagtctat tgttctgctt gcgtttctcg aatagcttta ttatactcga
    23341 aaaatccgct gaaaaacaat agacttttat aaatcattga tcaatcattc gaaagatttt
    23401 ctccatttga atcaattact tttttatacc agttgaatga tttcttcttc gaacgggcta
    23461 atgtgccatt tccctcgttg tcacgatcga cataaatgaa tccgtacctt ttcttcatct
    23521 cacctgtacc agctgatact aaatcaatac atccccaagt cgtatagcct aatagatcga
    23581 ctccatctaa ttctactgca tctttcattg cttggatatg tgctgccaga tagtcgattc
    23641 gataatcatc ttccacataa ccattctcat cgggcgtatc gactgcacct aagccgtttt
    23701 ctacaataaa tagtggtttt tgataacgat cataaatatc attcatcgtt attctcaagc
    23761 ctaatggatc aatttgccaa ccccattcac ttgcttccag atatggattt ttgacagaag
    23821 caaagatatt tcctgcagtc tgttccaaga gttcgttgtc agtagtcgaa actcgagaag
    23881 aataataaga aaaggagata aaatcaacag tatgttcttt cagcaatgcg gcatccccag
    23941 gttccatttc gatctgaatg ccatttcgtt ccatttcttt caatgcataa gcgggatatt
    24001 ctcctcgtga ctggacatca atgaagaaat aattttctcg atctgatttt ctagaagccc
    24061 aaacatcttc aggattgcaa gtgtatggat aatatgctcc tgctgccagc atacagccta
    24121 ctttgttctc cggatctact tcatgagcaa tcttcgttgc gatggcactt gcaaccaact
    24181 catgatgtgc cgcttgatat ttgatttgtt cttcattttc tccttcttca aaatacagcc
    24241 ctgcgcccat gaatggtgca tgaaggatca tattgatctc attgaatgtc aaccaatatt
    24301 tgacaagtcc tttatagcga ttgaagatga ctcggcacag attttcataa aaaccaacga
    24361 gttctctgga acgccacgca ccatattctt tgatcaaatg catcggacaa tcaaaatgtg
    24421 taatcgtcac aagcggctcg atcccgtact tttgacattc tttaaagaca tcctcataaa
    24481 atttcaaccc agattcattt ggctcttttt catcgcccat tggaaaaata cgactccaag
    24541 caatggacat ccgataagtc ttgaacccca tttctgcaaa taaagcaata tcttccttat
    24601 aatgatgata catatcgatc gcaatttttg ctggataaaa atgattttca tcaaattcaa
    24661 acattttctt ttttccagta atgacaggaa aacggtcttc tccgattggc acaacgtcta
    24721 cgttcgctaa cccacgtccg ccttcatcat aaccgccctc gcattgattg gctgctgtag
    24781 ctccgcccca taaaaaatct tttctgaatc ccatcatact tcctccatta catgtgtatt
    24841 gcttgataat tctcttgcca gttcatcttg gacttcgatt ccttctgctt ttaaccggct
    24901 gattgtttcc ttgaattgcg gtaaatactg ctgatgtgca ataaacaact catccattat
    24961 ccgttttgct gtctttcctg atcgtataag cggattgatc gtaaacgctt gcaaagctgt
    25021 tccataatca ccagtcacgg ctgcttcgat cgtcagcaat tccatattct tcattacttg
    25081 caaccagcct ctttcagcag caggaaggga accaaacgct acatttcgtg cgccttgtgc
    25141 gccaacatag gcagtgactt ctactacaca atctgccggc agatcaggaa cagctccatc
    25201 attctttgta gataccacga tctgtgtttc tttatttcca tagatcgaag caatcgtttc
    25261 acaggcagca tctgaataat aagcaccacc tcgtttttgc aattgttcag gtttatgatc
    25321 caattctgga tctttgtata attcaaacag ctctgcttct gtttctttca cttgctgcgc
    25381 tcttgtacca atcgtttgat attcttctaa agcatgttcc agcatttctt cttcacggta
    25441 gtaatagcgg tggtagccac aaggaatcat gttcatttgt tttaattgtt ctttgaaaaa
    25501 cgggatatca aagatatttt tcggcaatcc attgtcttca tcgtataaac gatcaatcaa
    25561 ttctgccgtg cggtcatttc ctttattatc tgctactttg tgccaatgga agtgatttag
    25621 accggcaaac ttgtagatca gctcttcttt tttcttgcca agcattgttg gttctgtcat
    25681 catcgccatt actggcacat tgcataatcc gatcacttta tcccatttgc catatctgat
    25741 aactgcttct gtcaccatcc cgcttgggtt cgcgaaattg atcagccaag catttggaca
    25801 taaacgcttc atgtcttcaa cgattgccaa aaccactgga acggtacgaa aagctttgaa
    25861 tatcccgcca gcaccatttg tttcttgccc aatcatccca taataactag ggatccgctc
    25921 atctttgatt cgtgcatcta attgaccgac acgaaactga gtcgtaacaa aatccgcatc
    25981 tttcaatgct tcttcccgat ccagagttgc atagattttg acttcatacg gagaagcatc
    26041 ccacatacgc tgagacatct gactgacgat ctcaagcttt tcttttcctt cttctacatc
    26101 tacgagccag atttctcgta tcggtaattc ctcatatctt ttgataaatc cttccatcaa
    26161 ctcgggggtg taactgctgc ctccaccaat cgtagcgatt ttgattcctt ttgccatgtt
    26221 ctcactcctt tactttttac acttttatta tcttttttgt aaacaaaaca aacaatagaa
    26281 attcgtttct ttccagtaac atttacaaaa gttagtgttt tcttctggat ttttgtaaat
    26341 atcttgtcaa aaaatgtctt tattaagcta tataacataa atccgtttac aaatcatttt
    26401 caaaaaagtt ataatttacg taaaaaaaca agaagaaggt atctacatgt tattacgtga
    26461 aaaaatcaaa gaaatctcct tttctccttc agaaaatgaa gtgattcagt ttctcttaag
    26521 cgattctcca gcacttggcg aaatgaccat ccaagagatt gctgagcgat gttatgtcca
    26581 cccgtccaca ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg
    26641 taatgctttt ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa
    26701 tcttcctttt tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga
    26761 acagatgacg attcaagata cactttctct cattcatcat gaccaactgc aaaaagccaa
    26821 acaactaatg cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat
    26881 ttctcaagat ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac
    26941 ttttggtgaa tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat
    27001 ttcttatacg ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatca
    27061 tgtgccaatt ttagctttga ccagtatcgg tgattcaacg ctatcttcaa tgagtacagc
    27121 cgctttgcga cttacgacac gcgaacgact gtattcgaaa attggtaatt ttacgatcaa
    27181 tacatcgatt tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca
    27241 acagaattta gtccatctaa tttctgtagg acaaaaattt gataaacgcc ctatcagctc
    27301 agccatcatg aaagagcctt tcaattttga aaataaagaa gtggactaaa aagaataatt
    27361 aagcctagtc cattctttca cctaaccgtt caggtcagtg cggtgataag tgcatcgtct
    27421 ggcccgaccg tctccccttg attttccaaa atatccaaat aatccgtgta attggtgacg
    27481 atcacaggtg tttctaaact gtatccttcc tcttcgattt tcttcttgtc gaagcggagc
    27541 aacagatcgc cttgtttcac tctttctcct tgctgcacca agcttttaaa atattttcct
    27601 tctaactgaa cagtatccaa tccgacatga atcagcaatt ccagacctgc ttcagaaacc
    27661 attccgattg catgtttcgt cgggaacagg gtcatgactg ttccgtcaaa aggggcacgt
    27721 acttctcctt tttcagggta gatcaagatt cctcgaccta atgttccttg tgcaaaagct
    27781 gcatcctttg ctgcattcag tggagcgatt tgtccttgta tcggtgtttt tacaatttct
    27841 ttacctgatg ttttctgtct atcatctgtt tcatctgctt cctccttcca aaagaaaaaa
    27901 gtcagcgcaa aggataccac catcgcaatg gcgatcccaa tcaatacatt aatcacatct
    27961 gttaaattac catctggtct aatatagttg ggaattgtaa agatgcctag tccaccttga
    28021 ttccaagaag ttaaattcat taacccacta tacgcgcccc ctaacgcacc accgacacag
    28081 gtaaaaataa atattttttt acgtggcaat gtaaacccat aaatagctgg ctctgtaatc
    28141 cccacaatac cagaaacaat ggctggtatt gcaattgctt tgtcttttgg atttctaagc
    28201 ttaaagtaga gtgcagtaag tgctgcagtt tgtccaaaag acactactgc aacaggtgtc
    28261 atgatatttt gccagccatt cacagaaaat tgcatcaatc ccattggtac gattgcccaa
    28321 tggagaccaa acatcattag aatttgccag aagaagccta cgattgctcc ataaataact
    28381 ggcgaaagtg ctaaaagtgt ttcaaatcca gccattaaaa tatcagttgc gaatgtgaat
    28441 gctggtccaa cgagtaaaaa acctaagggc attgtaataa gaacggtgaa gaatggaacg
    28501 aagaaattcg cgatcatctc aggtactaac tttttaattg gtttctgaat ttggctagct
    28561 aacaacacaa taaagataat gggaatgaca gaagaagcgt agttacgcgc aatccaggga
    28621 atgccgaata ctgtttgata aataggtgct tcaaaaatag tgccagaaaa tatggtcgtc
    28681 agtggtgctt ctgctgcacc actcaaagct tccagcgaca agctaggatt cattaatgct
    28741 gccccaagca acatccctac gaattcattt actttaaatt tacgggcagc tgttaaaccg
    28801 ataataacag gcataaattg gaatacagaa tcaccgatat tatcaaacat aacaaatgtg
    28861 ccagaaccgc ggtcaaatgc gcctaagaag acaagtaatg ctgtcagtcc acgaatcatc
    28921 ccaccagcag ctagaatagc aaggaatggc tggaaacagc ccgatattcc atcaataagc
    28981 atatcaaacg gtctcatttt ttctgttgaa acagcctcat caaaagtaag gtttcctgct
    29041 cttataacct cttcaaacac gactggcaca tgattcccga tgacaacttg gtactgtcct
    29101 cccgctcgca ttaccgtcac aacaccgtcc atatttttca agatctcatc atttgctttt
    29161 tcttcgtttt ttaatttgaa acgaagcctt gtgatacaat gtgtcaaacc attgatattt
    29221 tccttcccgc cgacattttc tacaattttg ctcgctaatt cttgatattt tcccatttct
    29281 attccctcca atttattagt ggaaggttta gccaactgaa tgtcactacc cttatttcat
    29341 gcgttcttgt taatttatcc tcctttttta tttatttaca acgattctca tgcactaaac
    29401 gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct
    29461 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
    29521 taacttggta caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
    29581 gtgtcagaaa aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
    29641 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca
    29701 ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
    29761 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
    29821 tttccccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
    29881 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
    29941 tatacactga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
    30001 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttctgaaa
    30061 gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
    30121 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatctc
    30181 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat
    30241 gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
    30301 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
    30361 ttatattatc gaaagcaagc gcttttgtca acgctttctt agtggctatt aggcacttct
    30421 gactagctgt cgaatctttt ttaaatctgg tgactacgac actaccgctc acagatgaag
    30481 aatctgggaa gctctgtttt attttcactc tagcgcttag gtcaaaatgc tactatgcta
    30541 ctgtcccaac ttctaatata cggtgttcca gtatcagctt atcggtgata agccaaattt
    30601 atgcaaaaat ctggaaagcc ttttttacaa tttttttctt atcacctaaa gctgaacgga
    30661 gccctgtcac aaccactttt tacacaccac tgctttgcag tttgtacata tctgcataca
    30721 aaccattttg cgctagaagt gtttcatgat tcccgcgttc tactatatga cctttgtcta
    30781 aaactaaaat caattctgca tcccgaatcg tggacagccg atgggcaatc gcaatcgttg
    30841 tccgtccttg tcgcattttt gtcaatcctt cttggatcaa tccttctgtt tcagtatcga
    30901 tatttgctgt cgcttcatcc aatactagta ttttaggatc tgtcacgatt gttcgcgcaa
    30961 aagtgatcag ctgtcgttgt ccgctagaat agctggcacc gtgttcgatc acttttgagt
    31021 gatatccttt tggtaattca tcgatgaact tgtttgcttg aacaaactca gctgcatggc
    31081 gaatctgttc gtctgtgata gttggattca tcatccggat atttcctgcg atatctccat
    31141 aaaacaagaa ggcatcttgc aagaccaatc ccattttctt acgtaactct tccattggat
    31201 attctcgaat gtcatgatca tcaatcaaga tctggccttc ataaaattcg taaaaccgca
    31261 tcaatacgtt gatgatcgag ctttttccgc ttccggtgtg ccccactaat gcaacggtct
    31321 ctcccggatt agcgacaaaa gagatattgt tcaatacatt gtgctctcca tcataagaaa
    31381 agcttacatt gcgaaactcg atcttacctg aagtaatcgt ttgatctgct ccaggatttt
    31441 gttgcggtgc atatttttct gtatccatga ttttcaaaat acgactaccg gcaaccattc
    31501 catctgtaaa ggtgctcaaa aagtccatca tttgagacat tggattaaaa aatccctgga
    31561 cataggtcgt aaatgcatag atcagtccaa cttctacggg agaatgcagc gcatcgatcc
    31621 caaacaaagt caacgccaac gcaatcgcca atgcatatag aaaattgatg atcggactta
    31681 acagaatcga attcgttttg atcatcgcat agcgcgtgtc taaatactcg ttgttcactt
    31741 gttcaaattc tttagctaaa cgagcttctt ggcgaaactg ctggataata cgcatgccgg
    31801 aaatcgattc attcaatttc gtgttcagct gacttaattt ttcccgcata ttccggtaaa
    31861 tacgagaact gaatttctgg taataccaga tgacgattcc taaaatcggc aagaacacca
    31921 aagtccacaa ggcaatttct ccattgattt gaaacatggc aatgaaggaa gaaacgactg
    31981 caaagatccc agtcaatacc atcaaaaaga cataccaaaa ttcaaatagc gtttctgtgt
    32041 catttgtcac tcgagaaacg gtcgatcctg ccggtgtctg atcaaaataa cgcataccta
    32101 gtgtatgcag cttttcaaat aatcttaccc gaacatactg ataggtcttc aatgaagcca
    32161 tggaatacaa ataccattgg aaaaaccaaa tgatactttt gactatgact ccaaacaagt
    32221 acataccagc aaaaaacaaa ataacttgtg tcgtagccga ctgtttagcc agatgatcat
    32281 ccataaaagt ttggatgatc cttggcaata ggacattgat cacagacaga gcaaacgcaa
    32341 ataagatagc aacgatgaac gttcttctaa aaggtttggc gaacttcatc agtcgtttga
    32401 tgatcttctt ctgttcttta agtggaatcg attttgtcca ttccgattga taattttcat
    32461 ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc tgtttctccc acattcgttc
    32521 ataccagccg cctaatgcaa gcagctcatg atgggttcct cgttcaatga ttttcccttg
    32581 atcgacaaca atgatttctt ttgcatgcat cacgctgctt aaacggtgag ctgcaatgat
    32641 ggttgttttc tcttttctta tttcttttaa attatgcaag atagcttctt ctgttttggc
    32701 atctactgca gaaagtgcat catctaagat cagcaattca ggatttttga tcaatgctcg
    32761 agcaatcgaa atcctttgtt tctgtccgcc agataaagag actccacgtt ctccaactaa
    32821 ggtatcataa ccttttggca attccttgat ttcattgttg ataaatgcta aagaagccgc
    32881 atgctctact tcttcttctg aaagttttgg attagaaaaa cggatattat ctcgaatggt
    32941 catcgaaaac aagaagtgat cttgaggcac atagccgatt gatcctaata aagcattcaa
    33001 tgaatattct ttgatcgaac gtccgccaaa ttggattttt ccttgataat catcgtattc
    33061 acgcatcagt aatttgatga tcgtcgtttt tcccgcacca gttttcccaa caatacctaa
    33121 tgtttctcct tcttggatca caaatttgat ttgttctaat gctggtgatt cttctttcgg
    33181 atagttaaaa gaggaaatag ctacattcag ctcgcctttt gccggggtct gcactgcacc
    33241 cagttgttcc acgatatgcg tcttttcatg caaaagttca ttgacgcgat cataagaagc
    33301 atttcctctt tccaatacgt tgaacaatcg accaatcgca aacatcggcc aaacaagcat
    33361 accgatatag ctaataaatg agaccatctg cccgatcgag atcgtgcctt ccaagacata
    33421 ctttccacct aaaataattg ttgcaacata agacagaccg ataatcaagg taatgaatgg
    33481 atcaaataag gagtctaaaa aattgactcg tttgttttta ataattgcat cttcaatctt
    33541 ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat gttttaatga ctttgattcc
    33601 agtaatactt tcttgtgtct tatcattgat actagaaaaa gcttcttgcg aatcacgaaa
    33661 agcatcgtgg agttttgacc ccagcactcg tgaagtaacg gctagtaaag gcagaggaat
    33721 cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc atcgcaatga tcgttgttcc
    33781 gcccgtgatc actgaatctg caaaggtcaa gataccagct cctgctacat tttggattgc
    33841 attcaaatca ttcgttgcat gagccatcaa atctccagtc cgatattttt ggtaaaagag
    33901 attgtccatt tctgtaaaat gtttgaatag ctgctgccga agttctttct ccaaccgagc
    33961 tgcacttccc caaatattcg tccgccagat ataacgaaaa agatattgta agagagctgc
    34021 aaggatcaat aataagatcc accaggaaat gatctttatt gataaatttt tatcggcaat
    34081 ctcatcaatg atgatcccaa tcacctttgg aggaactaac tgtaccaagg ccactgcgaa
    34141 tagggagaaa atcccaatta aataatgttt cttttcctgt cgaaaaaacc aacctaattt
    34201 tctaaaaatc gacattttac tcctcctttg tctttatcaa gagcatagga ttgtctgtgc
    34261 ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt acgaaataaa aaacggactg
    34321 aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat tttttccctt ggttcttcat
    34381 ttgctgcatc atctggcgaa ttttcttctc agatggtttt tgccccattg acatcatcat
    34441 tgatcgcaac atgtcctcat taacgggagg atttttttct aaatagtctt tcatatattt
    34501 acgcgctagg aaaaagccac ctactgcgcc gattagcaaa gcaataatag caataagtac
    34561 gacaatacct gttgatacca tataaatctc tcctttctat ttccgaattg ctctcaatga
    34621 tttattttac taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc atgcaaaaaa
    34681 tagtggtttt tttagagaag aacaaaattt ctttcattaa ccagcaagac acgccacgaa
    34741 aaaaaattag gcataccgaa tatttttctt taattttttc tttctgaggt gtagaatgta
    34801 atcgtagata aataggaaag gtggtttttt cttgaataat caatccaaca aacaatttca
    34861 taagcatcga cttattgaac atacaaatgg tttatcttta gctgaagtaa atggaacagt
    34921 ggatgtaccc aagggaaaaa gtttttttaa aacacttctt gcttactctg gaccaggtgc
    34981 attagtagca gtgggttata tggatccagg taactggtcc acttcgatca ctggtggaca
    35041 aaatttccaa tatttattga tgtcagttat tttgatgtct agtctgattg ctatgctttt
    35101 acaatatatg gcagcaaaat taggaatcgt gacccaaatg gatttggccc aagcaatccg
    35161 agcacggaca agtaagacat tgggtgtcat tttatggatc ttgactgaat tagccattat
    35221 ggcaacagac atcgcagaag tcattggtgc agcaatcgcc ctctacctgc tttttgatat
    35281 tccattagtg attgccgtat ttatcactgt gttagatgtt ttcgtcctct tattgctgac
    35341 aaaagtcggg tttcgtaaaa tcgaagcttt ggttgtctgc ttgattttcg tcattttatt
    35401 cgttttcgtc tatcaagttg gcttgtctaa ccctgattgg cacagtttgt ttgccggctt
    35461 tatccctaac agtcatacat tctcagactc accggtcatc ggcggtgaaa ctccgctgac
    35521 tggagcttta gggattattg gtgcaacagt catgccgcat aacttatatc ttcattcagc
    35581 agtttctcaa actcggaaga tcgatcacca agatgaagaa gatgtagcac aagcggtacg
    35641 cttttctact tgggattcta atatccagtt gtcaatggct tttctagtca atgcactgct
    35701 attgatcatg ggtgtcgctg tattcaaaac cggttctgtc caagatcctt ccttttttgg
    35761 tctgtatgaa gctttatctg atccatcagc tatgagcaat ggtatcttga ttgctgtcgc
    35821 aaaatctggt atcttgtcca ctttatttgc tgtagcactt cttgcttctg gccagaactc
    35881 tactattact ggtacgttga ccggtcaagt catcatggaa ggcttcatcc atatgcgtat
    35941 cccgatttgg ctaagaagac tagtcacacg attgctttct gtgattcctg tattgatttg
    36001 tgttttgttt acaagtacaa aaggaacgat cgaagaacat accgctttga ataacttgat
    36061 gaatgaatcc caagtttttc tggcttttgc attgccattc tcgatgattc ctttactgat
    36121 gatgaccaat agccaagcgg agatggggag ccgattcaaa aattcatttg ttgtaaaact
    36181 acttggctgg ttctctgtga ttagtctgac ttacttgaac ttacgaggat taccaggtca
    36241 aatcgaaagt ttcttcggcg agcaagccac ctcagctcaa ctagcattgg ctgatcaaat
    36301 cgcttatgta atcattgttc ttgtccttct cctgttggca tggacgattg gtgatttata
    36361 taaaggaaac aaaaaatatg aacagcgttt gcaagaaacg atagaagaaa tcgaatagaa
    36421 actgattctt ttacacaaag gaggttgtga cagaagtggg cagcttcaag aaataagaag
    36481 gaatttaaga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt
    36541 tacttctgga acaccgtgta tcagaggttg tgaaaaagct gtcctgcatc aagtaataag
    36601 aacagccaaa caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat
    36661 gcaagctttt gaacaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt
    36721 tcccttttta tttagaagat aaagcttttt tcagattcaa tttactgaaa actagcatgc
    36781 ttgttactac gatgaattgt gttaatggca cgcctgcgcc agtgtagaca cttaaaaaat
    36841 aatccttttt ttctggcgca taagaacaaa accacgagac accgaaaaga aaaaccaatt
    36901 gtgtcagtat taatccggtg actacaatca aagaggaact ataataggca cgatcagctt
    36961 ttgcaaatac ggttcctagt atcataagta atatccctgc tattgaagca atcagcacgg
    37021 gaatggccag aacttggtaa atttttatca tgacattcgc gacttttgct gtttttgtca
    37081 actggaaatc aggtgttctc ggatcactgc tatcgatcca attatccatt agtggaagat
    37141 tcaataattc ttgggcctgt tcgacttgtt cttgcgtccc gaaactataa ttccctcctt
    37201 gatggaaatc tttgtatcgc catgcatagg catatcctga tttcagaaaa gcaaatacga
    37261 ttggcagatc attcatcgtc tttccatccc ctgatcccat cagatagaat tcttttttct
    37321 ttttgatagt tcccttttga taggcttctt ctaattcttg attgactttt ttccaaaaat
    37381 cattggtttc ttttgcattg ttttggtaat aaccagattc gcttaccaca tctcgcaatg
    37441 cccaaaaaat gatatctcct ggtaattctt ttacttttcc gccttgggtc cacgcatgag
    37501 tcgtatacaa ttcgtcaata cgagtagaaa cagtatgaaa tgtaggagat gcgtctaatg
    37561 ctttatttaa tgcagcatgc gagacccata tctcactgtt ttctttattt aagttcgttt
    37621 cgtcatctat tcggatcagc tgtttcatca aacgtccaaa ttcaccactc gaacggtcat
    37681 tgatggtggc aataccatat gttgtctcgt ttttatattc gatccactga tagacgatgg
    37741 agaaaccgac tagagggagt aaaaacaatg aaagtttcag ccattcttgt ttcgaaagga
    37801 gaaatggtcg ttcttttaac ttctttgctt ctttgaaaga aaagtcttgt cctagcaaga
    37861 tctgaacgaa actgatgcct aacgcgacta cagtaaaagg aagaagccag atggaatctt
    37921 cgcgaatgaa ccaaaagaaa ggaaaagaaa cagctaaccc taaaaaccac ggcagcaatt
    37981 ttctgcggga actatattta cggtaataaa tagctagtaa acaagcgata atgatctcaa
    38041 cagccgggat cacgagagca ttacgatacg ttcgagtgga atactcatgt gtaaaactaa
    38101 tcggagaaaa caaaaaataa aaatagatac ttaaaagaag atacttgttc ttcacgatcg
    38161 gtttaaacgc tagtgcaacc aatatgctgg caagaatact gaacatgccg aacaaaactg
    38221 cataagaaag atgcagttta tgtgcaagta ctaaaaacaa gctgtaggag atcccttttg
    38281 aaagtgtcgt agtcgtgtac tcccctagcc atttaccatc caaaatattt tgagcatagt
    38341 gaatcagcag ctgatcatca taggttgccg acatatctgt tccatagatt gctttactat
    38401 ataaatacaa ccgaataatt gtaaataata cggctatgcc aagaataaag tatgtatgtt
    38461 tcttttttag ttggttgatc aagggtttta aagtgttcag catcttttcg tcctttcaat
    38521 cacgttttgc tacttgcttg tgaatctttt cgttttttac ttttctatca gtataccctg
    38581 tttgtctgaa tttaaaaaga gggggcgaaa ggagcatggc gaaaacgact gcaaaaagca
    38641 ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc ccaatccttt tttgttgctg
    38701 tcagatagaa aacttaaagt gtgacttctt ctgctaataa ctcaggaaac acttcgggat
    38761 cgaatgcttg cgctttgaac atggcaattt cttttccata tggtgcgtaa gctttctttt
    38821 tatctttacc gacatatgga gtttctaaga ttttcgaaat gttgtcaaac attggatcat
    38881 ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa accgatattg gcatgccgat
    38941 ctttatgaga accttgtgga tttttagaat cgttgatatg gagtacttta agccgatcta
    39001 atccgatgac cttatcaaac tcttccatca cacctgagaa atcttctttt atattatatc
    39061 cggcatcatt cgtatgacat gtatcaaaag taacggataa tttttcattc aattttactc
    39121 catcgatgat ttgcgctagt tcctcaaatg ttcgaccgat ttccgtacct ttccctgcca
    39181 tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa aacttcattc aaaccctcaa
    39241 taattctagc aatcccacct tctgcccctg ctcctacatg agcgcctggg tgaagggtga
    39301 tttgagtagc acctaaagct tccgcacgaa caatctcttc tctcagaaat tgtacagcaa
    39361 atggaaacat ttctggtttg atcgtatttc ccaaattaat gatatagggc gcatgaacga
    39421 cgatatttgt tagtccgttt tctgccataa atgcttttcc ggcatcaata ttcatctctt
    39481 cgatagcttt tcttctagta ttttgaggag cgcctgtata gatcataaag gtcgaagatc
    39541 cgtaactggc tgcttcttct gccgcgccta acagcatttt ctttccgctc atgctgacgt
    39601 gtgagcctaa taacatggat ggattcctcc ttgaaaggca attgtttcga acaattccta
    39661 tatattggta agatgatgta caaagaaaat ggtttgtttt tctttgccct gttctccata
    39721 gtaactgaaa ctttctctaa agaaaagctt aagaactctt tcataaaaaa gatcatttcc
    39781 tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa acagccttgt ctgttttttt
    39841 gtcttattat ttgctataat tagcgaaata aaaccattag ttgaggtgac ccttttgcca
    39901 cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag aaaaatggtt tgttcctaag
    39961 gttattttta gagtgtttca atcattgact gtttttatta ctgttcttct tttgttgttc
    40021 gcagctctag gtatcggaat aggtgctggc tactttgcct atcttgtcga agacacacaa
    40081 ttgccgacta aaaaagaact gcagacagaa ctaggaaaca ttacggaaac gtcaaagatt
    40141 gtttatgcag ataatacaga gatatcaaaa atccagacag atctgatgcg gacgactatt
    40201 tcatctgata agatttcgcc tttattgaaa accgcgatta tcagtacaga agatgaatac
    40261 ttcgataaac accaaggtta tgtccctaaa gctgttttac gagcattagt ttcagaagct
    40321 actggcatcg gctcgtctgg aggatccact ttgacacagc aattagtcaa acagcagatc
    40381 ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga tcgttctttc tgcacaagta
    40441 gaaaaatact tttcaaaaga tgagatcatt gctacgtatt tgaatgtctc gccatttggt
    40501 cggaataata aggggcaaaa tattgctgga gtccaggaag ctgcaagagg aatctttggt
    40561 gtagatgcaa aagatgtgac tctgccacaa gctgcttata tcgctggttt gcctcaaagc
    40621 cctatcacgt atagtcccta cacgaataca ggcgcgctaa aagaagatct ttctgccggt
    40681 cttgccagaa aagattttgt tttattcagt atgtaccgtg aaggacaaat tacgaaagaa
    40741 caatacgaag aagcgaaagc ctatgatctt acaaaagact ttctcccaca acaaatcgcg
    40801 gaacagactg acagagaatt cttgtattat accgtgatga acgaagcaac aaggattatt
    40861 gcacaacaat tggcagaaaa agataatgct gatatgtcag attcatcggt cagtgaagct
    40921 tattatcaaa aagcacagca aacgatccaa aacaaaggat atacgatcca ttcgacgatc
    40981 gataaagata tctatgctgc attgcaaacc ggcgtagaaa attacggcta tatgttagat
    41041 gatggtgctg gtagccaagt ggagactgga aatgtattga tggacaatag gactggacgt
    41101 atctatggat tcgtgggtgg ccggaattac agccagaatc aaaataacca cgcctttgat
    41161 acagaacgtc aagctggttc atccatcaaa ccggttcttg tttacggtcc tgcaatcgat
    41221 atgggacttg tcggatcaga atcccgtgta tcagattatg caacgacttg gcaagaagga
    41281 gaaaatgctg gcgagaaaat cgtcaatgcg accaatgaag ggtcgaacac cttccaaacc
    41341 gtccgtgagt cactggaatg gtccaacaat atcccagctt atcacttata ccaagacgta
    41401 ttgaattcgg gtggatcgaa acaatatgct tacgagcact acttggcgaa gatgaactac
    41461 ccagcaaatg ataattgggg cgttgaatct gctcctttag ggacagtaga tgtaacgacg
    41521 cttcaacaga caaatggatt ccaggcctta gctaatggtg gcgtctacga agaaggctat
    41581 atcattgatt ccatcacaga taatgcaggt aatgtgattt ataaacatga aagtaattct
    41641 gtccgtattt attccgaagc aacggcctcg atcatgaatg acatgatgcg cagtgtgatc
    41701 aatgaaaaaa tcactacacc attcaaagat gtcatttcaa gcttaaacgg aaatctagga
    41761 aaagcagatt gggtcggaaa aactggttct accaacgaat acagagattc atggctgatc
    41821 gtctctactc cgtctatcac tatcagtagc tgggcaggac acgatgacaa tactggaatg
    41881 gacagccaag caaggatacg ttcggctaat tatcttgcca atctaatcaa tcaagcttac
    41941 caagcaaacc ctgagatttt cggcactgat gagaaatttg acttatcatc tgatgtgatg
    42001 aagaaaaaag tatctgggtt cacaggacaa acagctggaa aagttactgt caataaaaaa
    42061 tctatccaaa cgccaggaaa aacagtggaa tcactttggg caaaaaatgg tccgaagaaa
    42121 agcgccttta aattcggcgt tggcggtact gatgaaaact atacagatta ctggaacacc
    42181 gcttcagctt atgctagagc gaatccagag accaaagaat cagacgataa agatgattga
    42241 tctaatagat taatcagaat taaagggagc tgcgacaaaa atcttgtcac agctcccttt
    42301 ttccgaataa atggtgttca aaagcttgca tctacgatat taagacaaat ttatgtaaaa
    42361 atgtgaaaag ccattttcac aaaatttttc tatataactt tactaacgaa actgttttaa
    42421 tttcttttct tgtctttgga ataattcggt tagtttttct gcaaattgcc cttttactga
    42481 ttctttcaag tgtacatgtc ggttagaaaa ttcttgagca gcttcttctg cagtcatttg
    42541 gtggttttga tctgtcaatc gtttttctgc ccaaatttgc tgagatatcc ccataccaac
    42601 tgaacgtaca tttgatcgat gtagattttt ctctaattct tttagtaatt gcattaaaaa
    42661 ttcattcata agatgatttc tccatagtta tttgtctgat tttttgacga taggaacgtt
    42721 ctagttcgtc ttgctgctga tagagttttc gtcgttctac ttgtagggct tgttgataat
    42781 gctcctgata attcgaataa cttcgtcttg cttgaaacag aggctcatta tctaaagcta
    42841 agttctttaa tacatgatgc agtcgctgct cctcttgctc catcaatcgt aatccttgat
    42901 tcttaaagtg gatcaattca tcttcttgag cttctaattg tcttcgttca cttcgataat
    42961 ctctttccaa ttcttctagt ttcatctact tctcctttaa ttgaattgct gtgctaattc
    43021 tcgatctcgt gtcacgattt cattgatttt gtcttggatc tccttcacca atgtatcaaa
    43081 ggcctccccc atacgtcttg ccttattgat tcgcagcaga tagacttctt tcgcagaatc
    43141 gactaagttc tcttttgtgg cgacaacact agctaatgct tctatgattt cctgttcagt
    43201 caacgaaggg gcgacaacac gtgcttgccg caagccttct tgccatgcca ttttttctct
    43261 agctcaaaaa ttcctctttg attgatgcgg ataacttctt ccatagcgct tttcattttt
    43321 gctgataact tttccactac taacctagct tgtgtgtcat ctaagtaaat ttgttcatta
    43381 actgtcagcc ggccgccact taatcgtagt tttttacgta tgttgttcag ttcttgcaac
    43441 tgataggaaa acaactgggt cgcttggttt tctttctgtt gttttcgagc tttaacatta
    43501 ttttcatttt caggaataat cagctttcca ttttcatcaa acttccaaga ggttaaactg
    43561 tgggaagcaa gcagattcaa gaatgctgtt tccatactga tccgaacttc agcacctgtg
    43621 ccattaccac caaaattacc gataaaatcg gcgtgatttc ggtaattggt gagcataccg
    43681 ggattttctt ccacccattt ttttgcttct ggtgataaga gatcatacgg gtccggcccg
    43741 tttaagccga catttttcca ttgtttctct gctgctacat ataatgctaa gaatccacct
    43801 aatgaatggc cggtagtaga aatagcagct tctccatatt ccttttttac ttcattagca
    43861 aatgcaatcg ctgaattgat ttgccccgct gttttcaatc cactaacgga taaaatcttg
    43921 tttgttgttg ctttttcaga aaaacttgct cctggtctat tcccatagag attcatgata
    43981 ctccttgcat ccgtttccaa atctttccaa tcacttaagt tcgttcctgc aaaagcaatc
    44041 actacttgag aaagatccac tttaccgttc ttgattggcg ctacacccat cgcctgcatg
    44101 ccgtccaatg agtctttgat ttttaggatt ttaaaattat aactatcaat ttttttaata
    44161 gaattttctt tataagtatg atcatagtct ctgtgtttag gatctagcca gtaaacagcc
    44221 tcactaataa cactgtattg tttgtctgcc atcattaaat atattcctcc ttattattag
    44281 aaaaaattac tctgatttta tttactgtaa tcccctcttt ttgtatactt ttattttcta
    44341 cgccataatc tcctaattca tttcgttctt tccaaaatcc aaacgtaaaa taaacagact
    44401 catcgtgatt atttatcatt gtcacaaaca tacgatatgc tcccgtcatt tcatttcgag
    44461 cagatttttc aatcttcact tccttgatat ccgcaaaagt atttttcaaa actttgactg
    44521 actgtttttc tactgcgagt aaatctttct gtctttgtgc ttctcttttg tccatataga
    44581 ctttccctcc gattcctaat atgacgatta ataaaatggc taagctgagt tttcttattt
    44641 tccgagacaa gaacgtgcct cctttctttt tatgtaagta ttgatacctt cattatttaa
    44701 gtacgcgaaa gtttgtagga atttttttat ttttgaaatt tttctccaat ttgctatgga
    44761 cgattggcgc tgcagccatc gcctgcatgc catccaatga gtctttgatt tttaggattt
    44821 ggaattttgt ttctccaaag tttttaatgg cgccttcttt gtaactttta ctatattgag
    44881 gatgttttgg atctagccaa taaacatgtt cacttatata tctatactct tcgtctttta
    44941 ctgtcacact tcctccgcct ctccattaga ataaataact cttattttag gagtagtaac
    45001 cccttcgttt tgtatttctt cattttctac accataactg ctaatttcat tcatttgttt
    45061 tgaaaaaaaa taatcaaaat aaacagattt cccttcatta tttgtcattg tcacaagtgc
    45121 accataacat ccagtaacag ggtttttttt aaattcttct accttcactt ccctgatatc
    45181 tgcaaaggta tttttcagaa ctttgacgga ttgtttttct actgcgagta aatctttctg
    45241 tctttgtgct tctcttttgt ccatgtaaaa ttttcctccg attcctatag caagaatggt
    45301 caaaatgatg actatggtct ttcgtctttt cttaggcaaa tagaagtctc ctttcgggtt
    45361 atgtatgatg gattgtttct cctttaagat aagtacgcaa tcgagcctgg atttttttgg
    45421 attttttaga gttacttttc tacttttcta ctttcttttt tatcacgttt tcattttaaa
    45481 gtaattttga tagtagtgaa caaaatgtag catctacgca cagattgtgt cgtatacgat
    45541 ggacgtaagg tttgtttccg ccattttctc tttttttaaa gtcacaaaaa aaaggggtta
    45601 tgacaaaatt ttgtcataac cccttttctc gaataaacgt tgttccagaa gttggccact
    45661 tttggaacaa cctctaaatg attgatcttt tattacttag ttgatccttt ttcttcgatt
    45721 ccataaggca agataatcgt tttttcttcg acttcttctt tattcatcat tttcgtcaat
    45781 aggcgcatgg ataccgctcc taaatcgtat aatggttgtg tgatacttgt caaacgagga
    45841 cgagctactt ctgtcagtaa agaattgttg cttgtgatga tttcgaattc ttctggtact
    45901 tttacgccac ggtctaataa accatccaag ataccaatgg ctaattcatc gtctgaaaca
    45961 tagacagctg ttgcaccgct attatggatc cggtcaacta gagacagacc atctttaaag
    46021 ttgtattgtg cctcaaagat taatccttcg ctatatggta gattgttttc gcttaatgct
    46081 tctttatagc cttttagacg gttttgacca ttgataggat caatcagcgc accgctgaca
    46141 aaagcaattt ttttattgcc gctttttgct aatttattta ctgcatcttt tgttgctgat
    46201 acataatcaa tgtttacgct acctacttgt tcatctggat caatggaacc agctagtaca
    46261 acaggtgttt ttgaacgtga aaattctcca cggatttcgt ctgtgatatg gtgacccata
    46321 aagatcacgc cgtccacttg cttagccaat aaattattca atacattgac ttctttttga
    46381 tcgttgccat ctgagtttgc taagatgata ttgtatttgt acattgttgc tacatcgtcg
    46441 ataccacgtg ctaaagaggc aaagaacata ttgctgacat caggaatgat taccccaaca
    46501 gttgttgttt tcttacttgc caagccacgt gcgactgcat ttggacgata atccaagcga
    46561 tcgataactt ccaagacttt ttttcttgtt gctggtttga cgtttggatt gccatttacg
    46621 acacgtgaaa cagtagccat cgaaacattt gcctctctcg ctacatcata gatagtgatt
    46681 gtttgctttt ccatttgtgt tctcctattc atattttata tgttttctga aaatacgtct
    46741 tacatttcta aaataggata tcaaataatc tcaacaattg caaccgtttt atctctgttt
    46801 tcatgaaaat ttttactttt ttcagtttta agaacgttgg tcttttaatc aatcagtggt
    46861 acaattattg aatacaaaat taatttagaa agtaggttgc caaatgaata acgtaaaaat
    46921 caatgaacta cgtcaatgga tggcacaaca ccaagtagaa ttggcttata tcagcgaccc
    46981 tggccacatc gcttactttt ccggatatga aagtgatcct cacgagcgtg tactagctct
    47041 ttttatccct cttgaacaag atcctttcct tttcactcct gctctggaag ttgaagatgc
    47101 tcaaaacagc agctgggcgt acgatgtacg tggctactta gacagcgaaa acccttggga
    47161 gatcatcgga aaagagcttc gcgcacgcta tgggcaacct ggtaatgtag ggattgaaaa
    47221 gaatgctctg actgtcgatc gcttcgatgc tatctctgat atcttgacag gtgcagattc
    47281 ttacactgat ctaacttcta ttatccagcg tctgcaactg atcaagacgg tagaagaaaa
    47341 agacaaactg atcgaagctg gtaaatgggc cgatgttgcc tttgaaattg gcttcaaaac
    47401 agtaaaagaa ggcgtgactg aacaagcaat tatcgcagaa atcgaatacc aattaaaaaa
    47461 acaaggtgtt tcacaaatgt ctttcgacac cttagtcctt gcaggagcaa atgctgcaag
    47521 ccctcatggt acaccaggaa gcacgaaggt ttcaccaaat gaactagtgt tattcgatct
    47581 tggcgttgtt tggaatggct attgcagtga tgctactcgt acagttgctt atcaaaaacc
    47641 aacagaattc caagaaaaaa tctacaacat cactcttgaa gcacagcttg ccgcacaaga
    47701 ggcagttcgc ccaggtgtga cagcaggtga acttgatcaa atcgcacgaa acgtaatcaa
    47761 cagctatggc tacggcgaat atttcaatca ccgtttaggt cacggaattg ggacaaccgt
    47821 ccacgaattc ccttctcttg tagaaggaaa cgacttagtg atcgaagaag ggatgtgctt
    47881 ctctcttgaa ccaggaatct atattcctga aaaagtaggg gtacgtatcg aagattgtgt
    47941 gtatgtgaca agtgatggtt gtgtgccatt cacgactaca ccaaaagaat tacttgtttt
    48001 agagtaatga aaataaaaaa ggtgggagct ggtccgctct caccttttta ttctagaaat
    48061 gagtattctt agtgattggt agtcgaatct gtcattgtat ctttcatttc ttttttagct
    48121 tcttttgcat cttcttttgc cattttcaac atttcttttg tgtcatctgc aactggtttt
    48181 gcttctgaca ttccttcatt gaccgtctct cctgctttat ctgcagcatc ttttacatcg
    48241 atcatgatat cttctgaagt atctttagca atactaccta attcgtctgc tggttttttc
    48301 agatcttctg ctgttttttt gaaacgatta gataaatcag ctgattgttc tttgaggttt
    48361 aacaagatat catctgattc acttcctgct tcctctgctg attcacttac cttttgctta
    48421 acattacctg ctagatcccc tgcttgttga gaaagatctt gcgctttttc ttttgcaatg
    48481 cctgttaact cagctgattt ttcttttgcc atatctgtgt aatctgaagc catgtccatc
    48541 aaatcatctg cttgagcagc caattcatcc cgaagttctt taccagattt gggtgctaac
    48601 aataatgcgg cgattgctgc ggctgtacct ccaactactg cacctaataa aaatccacct
    48661 tttttagcca ttcttatttt cctcctgtcg tttcagtatg ttttttgttt ttaaaaaatt
    48721 tcatcgctgt tccaccgact tttccggcaa cagtcgcttt agctgtcgtt tttccgattc
    48781 cgccgacttt cgtcacgatg tttttactag aatgattaag ttccgataca ctttcactta
    48841 aatcagcaac tgctgtgaac aatggatcga tcgttgcgac ttttccattg atatccaaga
    48901 gcagctcatt acttttgacc agcaagcctt ctacttgtct agataaaaga tctacgtctt
    48961 ttgtgacgat ctcaattgtc gtatttgctt ctgcgacagt ctggtctact ttttctaaag
    49021 ttttggaaac ttgcaacaat acgcgaatga tgaaaacgac aagtatcgca aaggcgattg
    49081 ctgcgatcaa tcctgcaatt tctcctcctg acatgatcaa actcccttct tgtctctctt
    49141 ttctataagg atagcatttt cgtgtttttt aaacaaataa aaacgagctt caactacttt
    49201 ttattcaatt agcagctgaa gctcgttaga aaaaaatgat tattttttct tttcttcttt
    49261 gatttctgtt attccttttg gattatgatc gatcttgctt ccttctgctt ctaatgtttt
    49321 acctaaagac atgatgtatt cacgcaaagg tgcccctact tctggatgtg tcagaccata
    49381 ttcaatactt gttttcataa aaccaaattt gtcaccaacg tcataacgtt tacctgtgaa
    49441 tttacgggca aatacgcgtt gtgttttatt caacgtatcg attgcgtcag ttagttggat
    49501 ctcatttcca gcacctggtt gttgttctga taatacatca aagatttctg gagtcaataa
    49561 gtaacgccca ataatagcta aatcacttgg tgctttgctt ggatctggtt tttcaacaaa
    49621 gtttttgaca ttgtataacc catcttccag tacttcccct gggttgataa ttccgtattt
    49681 tgaagtttca tcgtgaggaa cttgcataac ggcaatcgta gaagcgtgtg tacgttcata
    49741 gtcatcgatc aattgctgtg tcaatggtac ttcgtcttcc atgatatcat cgcctagcat
    49801 aacgacaaac ggttcatttc caataaaggc tcttgcttga agaacggcgt gtcctaaccc
    49861 ctttggatga gattgacgga tgaaatgcaa gttgacatct gtcgtttctt ctactaattt
    49921 caataattcg atttttcctt tttcacttag gtttgtttct aattcgatat ttgaatcaaa
    49981 gtgatcttcg attggtcgtt tagcttttcc tgtaacgatc aagatgtctt cgattcctga
    50041 tttcaacgct tcttcaacga tgaattggat cgttggtttg tccacgattg gcaacatttc
    50101 ctttgccatc gcctttgttg ctggtaaaaa tcttgttcct aatcctgctg ctggaataac
    50161 tgcttttttt actttcatat acattcgctc cttcaaaaaa ttgttaatga gaattcattt
    50221 tcttttttcc catcacgtaa catgatttct tttgcggctg ttttgatttc tttttgttca
    50281 tacagcactt catagattgt ttgagtgatg ggcatttcga tttccatcat ttgggctagt
    50341 tccatcgctg cttgagtagt attcacacct tcaacgacca ttcccatatt ggctagcacc
    50401 tcttctagag gctgtccttg tcctaacaag ttgcctgcgc gccagtttct ggaatgaaca
    50461 cttgtgcaag taacgactag atcgcctact ccgcttaagc caataaaggt cagcgggttg
    50521 gcacccattg ctacacctaa acggctgatc tcagctaatc cgcgcgtcat gattgccgct
    50581 tttgcattat cgccaaaccc taagccgtga atagcaccag ctccaatggc aataatattt
    50641 ttcaaagctg cccccatctc tacaccaacc acatcagggt tagtatagat tcgaaaataa
    50701 tggttcatga acagctcttg tacatatttt gctgcttttg catctgtgct tgccgctgtg
    50761 atcgtcgtaa tgtcatgctt tgctacttct tccgcatggc ttggcccaga aagaacagca
    50821 acagcttttc ttttttctgt cgggatttcc tctaacaaga tttcagagat tcttttatgc
    50881 gttccctgtt ctaatccctt acttgcgtgg atgatcactg gcttcgtatt caatttggca
    50941 actagttctt ttgcaacaga acgtatcgct ttcgtcggga taacgaatag taccgcatct
    51001 gcatctttta ctgcatcttc catatcggtg aatgctcgga tggaatcggg tagatgatga
    51061 tctggtaaat aacgttgatt ggtgtgttgg gtgttgattt cttctacttg tgaagcacga
    51121 tgtccccaaa tccgaacgtc atgtccgttt tcagcgagga cttgtgctaa agcagtcccc
    51181 caagagcctg gtcctaaaac cgctactcta taattcattt taccactcct gaaatttttt
    51241 tcatttttaa cgctctaaaa atcaaataat ttgttgattt tttcctaatg agcggtcata
    51301 atccggtaaa tttccttttt ttctacgcca aataaacaga atcaatgtgc cgacaagtaa
    51361 aacagcagac aataattgag aaatgcggat cacacctaaa aagtaaaggc tgtctgtgcg
    51421 taatccttcg ataaagaaac gtccgaaact ataccacatt agataacata aaaggacttc
    51481 tccttttttc aaaagatgcg gtttcttccg caaaaccaaa agcagaataa atccaatcaa
    51541 actccaaagt gattcataaa gaaaggttgg ttgtcgatag atcccttcga tatacatgtt
    51601 atcaatgatg aattgcggta aatgcagact ttctaagaaa cttcgtgtcg tgtctggacc
    51661 atacgcttcg tggttcatga aatttcccca tcgtccgatt gcctgcgcca gtaatacact
    51721 tggtgcagcg atatctaaaa aggtccacaa agaaataaaa tgatatttgg taaaaaagta
    51781 cagtgtgatt gcacctgcaa tcaatccccc gtaaatcgct aatcctcctg aacggatatt
    51841 gaatatttgg attggatcag ctatatattg gggaagatcg aataaaacat aatacaaacg
    51901 ggcaccaata atggaaatcg gcaatcccca caacataaaa tctgtcacgt catctgcttt
    51961 taatcctacc cgaacagctt ctctgctgct gagccacatg gcaataatga ttcctgatac
    52021 gataatcaat gcataccagt agataggaat tcctacaagc tcaaaagcaa ctcgattgat
    52081 ttgtgctaac atattgtcac tccttgctag tcattgcctg agttttcttc aatcaagcga
    52141 cttaatcttt cttcaaatgt tttcgttgcg tcaaagccca ttgtacgcgc gcggaaattc
    52201 atcgcggcta cttcaatgat gattgcaacg tttcgtcctg ttttgacagg gatttttatt
    52261 tgtggaatgc ccacttccgc gatttcaacg gtcgtgtctt cagaacccaa acggtcgtat
    52321 ttcttatctt tcgcccatgc ttctagataa acagctaact ggacttgcat agagcctcgt
    52381 accgcactcg caccgaaaag attcattaca tctatgatcc cgatccctcg aatctcaatc
    52441 agatgttcca ggattttagg aggttctccg attaccgtca gttcatcttg ctgatagaca
    52501 tctactcgat cgtctgcgat caatcgatgt ccgcgcttaa tcagttccaa cgccgtttca
    52561 cttttcccta tcccactgtc cccttgaatt agtacaccta atccgtagac atcgactaac
    52621 acaccgtgga cacttgttcg aggggctaag cgcccatcta aataactaga taattcacct
    52681 agcagccgag acgttgagat aggcgaacgc agcacagcta tcccattttc attggctgct
    52741 tggatcattt cttccggagc cggcaatcct cttgaaataa taaatgcggg catatcttca
    52801 ctacataatc ttctcatgat gatcaaacgc tcttctggga tcatccgttc cgcaaaactg
    52861 atttctttac tgccaaatag ctgtaaacga ttatgagaat aataattaaa ataaccggtc
    52921 aactctagtc ctgggcgtga gatgtcccca gtcacgatga cgcggtctaa acttttctcg
    52981 tcgcctgtga cgatttccaa agataatttt tttactaatt ggctaacttt tacaacttct
    53041 gacattcatg ctacctcatt tctattagag tgttctctac cattttatca ttgtctagcc
    53101 aaaagtacta ccaattaaaa aaaacgtttt tatttaatta ccaatgtaat agaaaacgca
    53161 ttcttaatta tatatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct
    53221 caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat aattacgttg
    53281 attgctgaca ctggaaataa atgtctgatt cacttctcta aaatccattg atttcggcag
    53341 tccattacga cgcagaatcc cgttagaatt ttcgtttaat gggcgttgag aaggtgttcc
    53401 agggtccgca aaatatatat caatatcatg ttggttacta atggctttcc agttagaaaa
    53461 ttcttttcca cagtcaaacg taatagattt aaagaagttt ttagggaagc gagaaaacca
    53521 ttgattaagg gcagtttcaa tatctaatgc cttacggccg ttgggtttaa tcgtgataat
    53581 gactttagat aatctttcaa ctaaagtaat gacggcactt ttatgatgaa tgccaacgat
    53641 cgtatcacct tcaaggtgac caaactcaga attgaaatca ggataattat caggtcgatc
    53701 atggattgag cgctgatact gttgttttcc ccgtttttcc tgatggccat tgggttttct
    53761 tttacctttc atcggtagtg tgtcaatatc aaatattcct ttagaaaata aacgataaag
    53821 tg
//
LOCUS       DASHWH010000012        50930 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.012, whole genome shotgun sequence.
ACCESSION   DASHWH010000012 DASHWH010000000
VERSION     DASHWH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.012"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(33..830)
                     /locus_tag="U2R41_000719"
     CDS             complement(33..830)
                     /locus_tag="U2R41_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEM8888151.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(827..2011)
                     /locus_tag="U2R41_000720"
     CDS             complement(827..2011)
                     /locus_tag="U2R41_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM8888152.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2036..3283)
                     /locus_tag="U2R41_000721"
     CDS             complement(2036..3283)
                     /locus_tag="U2R41_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM8888153.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3498..5243
                     /gene="dnaX"
                     /locus_tag="U2R41_000722"
     CDS             3498..5243
                     /gene="dnaX"
                     /locus_tag="U2R41_000722"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEM8888154.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5263..5577
                     /locus_tag="U2R41_000723"
     CDS             5263..5577
                     /locus_tag="U2R41_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEM8888155.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5602..6198
                     /gene="recR"
                     /locus_tag="U2R41_000724"
     CDS             5602..6198
                     /gene="recR"
                     /locus_tag="U2R41_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEM8888156.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6262..6906
                     /locus_tag="U2R41_000725"
     CDS             6262..6906
                     /locus_tag="U2R41_000725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEM8888157.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6921..7250
                     /locus_tag="U2R41_000726"
     CDS             6921..7250
                     /locus_tag="U2R41_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEM8888158.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7264..8202
                     /gene="holB"
                     /locus_tag="U2R41_000727"
     CDS             7264..8202
                     /gene="holB"
                     /locus_tag="U2R41_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEM8888159.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8238..9062
                     /locus_tag="U2R41_000728"
     CDS             8238..9062
                     /locus_tag="U2R41_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEM8888160.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9055..9402
                     /locus_tag="U2R41_000729"
     CDS             9055..9402
                     /locus_tag="U2R41_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEM8888161.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9471..10343
                     /gene="rsmI"
                     /locus_tag="U2R41_000730"
     CDS             9471..10343
                     /gene="rsmI"
                     /locus_tag="U2R41_000730"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEM8888162.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10452..11573
                     /gene="dinB"
                     /locus_tag="U2R41_000731"
     CDS             10452..11573
                     /gene="dinB"
                     /locus_tag="U2R41_000731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEM8888163.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11627..12229)
                     /gene="nrdG"
                     /locus_tag="U2R41_000732"
     CDS             complement(11627..12229)
                     /gene="nrdG"
                     /locus_tag="U2R41_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEM8888164.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12544..14733)
                     /gene="nrdD"
                     /locus_tag="U2R41_000733"
     CDS             complement(12544..14733)
                     /gene="nrdD"
                     /locus_tag="U2R41_000733"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEM8888165.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15032..15826
                     /locus_tag="U2R41_000734"
     CDS             15032..15826
                     /locus_tag="U2R41_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888166.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15816..17858
                     /locus_tag="U2R41_000735"
     CDS             15816..17858
                     /locus_tag="U2R41_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888167.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18139..18288
                     /locus_tag="U2R41_000736"
     CDS             18139..18288
                     /locus_tag="U2R41_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEM8888168.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18299..19813
                     /gene="dltA"
                     /locus_tag="U2R41_000737"
     CDS             18299..19813
                     /gene="dltA"
                     /locus_tag="U2R41_000737"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEM8888169.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19810..21015
                     /gene="dltB"
                     /locus_tag="U2R41_000738"
     CDS             19810..21015
                     /gene="dltB"
                     /locus_tag="U2R41_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEM8888170.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21060..21293
                     /gene="dltC"
                     /locus_tag="U2R41_000739"
     CDS             21060..21293
                     /gene="dltC"
                     /locus_tag="U2R41_000739"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEM8888171.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21296..22564
                     /gene="dltD"
                     /locus_tag="U2R41_000740"
     CDS             21296..22564
                     /gene="dltD"
                     /locus_tag="U2R41_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEM8888172.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22705..23787
                     /locus_tag="U2R41_000741"
     CDS             22705..23787
                     /locus_tag="U2R41_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEM8888173.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23787..24590
                     /locus_tag="U2R41_000742"
     CDS             23787..24590
                     /locus_tag="U2R41_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM8888174.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFVFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24660..25400
                     /locus_tag="U2R41_000743"
     CDS             24660..25400
                     /locus_tag="U2R41_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8888175.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25411..26415
                     /locus_tag="U2R41_000744"
     CDS             25411..26415
                     /locus_tag="U2R41_000744"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEM8888176.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26460..28304)
                     /locus_tag="U2R41_000745"
     CDS             complement(26460..28304)
                     /locus_tag="U2R41_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888177.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28516..29034
                     /locus_tag="U2R41_000746"
     CDS             28516..29034
                     /locus_tag="U2R41_000746"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8888178.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29162..29845
                     /locus_tag="U2R41_000747"
     CDS             29162..29845
                     /locus_tag="U2R41_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEM8888179.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30154..30227
                     /locus_tag="U2R41_000748"
     tRNA            30154..30227
                     /locus_tag="U2R41_000748"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30188..30190,aa:Arg,seq:tct)
     gene            30386..30745
                     /locus_tag="U2R41_000749"
     CDS             30386..30745
                     /locus_tag="U2R41_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEM8888180.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            30868..32499
                     /locus_tag="U2R41_000750"
     CDS             30868..32499
                     /locus_tag="U2R41_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEM8888181.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32561..33202)
                     /locus_tag="U2R41_000751"
     CDS             complement(32561..33202)
                     /locus_tag="U2R41_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM8888182.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    33388..33464
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            33484..33792
                     /gene="rplU"
                     /locus_tag="U2R41_000752"
     CDS             33484..33792
                     /gene="rplU"
                     /locus_tag="U2R41_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEM8888183.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            33819..34163
                     /locus_tag="U2R41_000753"
     CDS             33819..34163
                     /locus_tag="U2R41_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEM8888184.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34176..34469
                     /gene="rpmA"
                     /locus_tag="U2R41_000754"
     CDS             34176..34469
                     /gene="rpmA"
                     /locus_tag="U2R41_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEM8888185.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            34903..37710
                     /locus_tag="U2R41_000755"
     CDS             34903..37710
                     /locus_tag="U2R41_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8888186.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            37901..38392
                     /locus_tag="U2R41_000756"
     CDS             37901..38392
                     /locus_tag="U2R41_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8888187.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38495..39484
                     /gene="manX"
                     /locus_tag="U2R41_000757"
     CDS             38495..39484
                     /gene="manX"
                     /locus_tag="U2R41_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEM8888188.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39517..40320
                     /locus_tag="U2R41_000758"
     CDS             39517..40320
                     /locus_tag="U2R41_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEM8888189.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40341..41255
                     /locus_tag="U2R41_000759"
     CDS             40341..41255
                     /locus_tag="U2R41_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEM8888190.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41338..41712
                     /locus_tag="U2R41_000760"
     CDS             41338..41712
                     /locus_tag="U2R41_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEM8888191.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            41851..42096
                     /locus_tag="U2R41_000761"
     CDS             41851..42096
                     /locus_tag="U2R41_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8888192.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42268..43332
                     /locus_tag="U2R41_000762"
     CDS             42268..43332
                     /locus_tag="U2R41_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM8888193.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43578..44003
                     /locus_tag="U2R41_000763"
     CDS             43578..44003
                     /locus_tag="U2R41_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8888194.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            43996..44445
                     /gene="nusB"
                     /locus_tag="U2R41_000764"
     CDS             43996..44445
                     /gene="nusB"
                     /locus_tag="U2R41_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEM8888195.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44542..45396
                     /locus_tag="U2R41_000765"
     CDS             44542..45396
                     /locus_tag="U2R41_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEM8888196.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45397..46734
                     /locus_tag="U2R41_000766"
     CDS             45397..46734
                     /locus_tag="U2R41_000766"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEM8888197.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46739..46963
                     /locus_tag="U2R41_000767"
     CDS             46739..46963
                     /locus_tag="U2R41_000767"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEM8888198.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            46965..47855
                     /locus_tag="U2R41_000768"
     CDS             46965..47855
                     /locus_tag="U2R41_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM8888199.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            47864..48682
                     /locus_tag="U2R41_000769"
     CDS             47864..48682
                     /locus_tag="U2R41_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEM8888200.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48789..49238
                     /locus_tag="U2R41_000770"
     CDS             48789..49238
                     /locus_tag="U2R41_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM8888201.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49256..>50930
                     /gene="recN"
                     /locus_tag="U2R41_000771"
     CDS             49256..>50930
                     /gene="recN"
                     /locus_tag="U2R41_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEM8888202.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAK"
BASE COUNT        16317 a         8748 c        10823 g        15042 t
ORIGIN      
        1 cccttttttt gcgaacattc atttagcaat ttttagaaac ttctgccgcc accgccaaaa
       61 gtcccgccac cgctagaatg ggtagtactt ccgccaccgc cgcttccagg aggtgggctt
      121 ttggggattc ttcttgtcgt gacaaaagaa tttgtcagac gatcggtttt ttcagttaat
      181 ttgatactgg atttttctcg gaatggatat ttgtagatgc ctgatttcaa ttgataacgc
      241 gaaacagtca caaaataaaa gacaagacta aggataagag ccaaaacaac agcaatgatg
      301 atttctactg tcgtcaaaac cttatagcgt gtaattttgc cagtttcttc gtctacacga
      361 taatgtcctc ctggaactcc gtcttccaca tatgttgctg atttagacag gtaagctttt
      421 gcagcattgt aataggcagc atttgtcatc tgatcataaa catcatctaa gatcgaatcg
      481 attctgctat cagtcaaata atcgatcata ttaccagaag tcgaaatgta gatttcacgc
      541 tgtcccatat ctaatagcaa tactgagcca ttttgatcat ttccgactgc gtctcttaag
      601 taattatcag caaagtctct tggttcttct gaattagatg tagtcgttac gatcaagact
      661 cgtcctttga ttttttcatt cagcttttct gcctgttgtt ccaaagattg gatttgatct
      721 gttgtgaata acccagcctc atcatttacc gtagctgtat ctgcatgagt agccaccggc
      781 cagccaacac ttactagcaa aagaaagaat aaaaaaaatt ttttcatcat atcaagtaac
      841 ctccgatcag aaagaggatc gctagcccag cgaaaatccc aagagcggtc agtcccaatt
      901 ttttctggct gatcggcagg acaccgctga cctttcctgt ttgtccattc attgcgtagt
      961 agtagacttt tttacttgag tcattgcttc gataagtaac aagccatacc ggcagtaata
     1021 cgtaattatt tacttcgttt gtgattgaag cagaagttcg cacaccggtc aatgttgtat
     1081 atccatttgc ggtatctttt aacagcttct ctgaatagtc ttttaattca gattgaatct
     1141 gtgatttgat tgcctcatat tcgatatctc ttttttctgc ttggaacccg gctaaatact
     1201 ggcttttaaa atcaattgct ttatctaacg ggaaaggttg cacgccttcg accatctttt
     1261 gttgcgtatt ttttgataag gcatttttta ctagttcttt aaaagaaagc ttgcctgctc
     1321 ttgaaacagc aaattgcttc gtttctgtgt attcgatgtc acctatccgc catatgcgga
     1381 taactgtacc attcgcctgc atggcaccat ctacttcagc atctgtagcc caataaggga
     1441 aatacacacc tgtcaactta tcgatttgat ccttattgaa aaaagctttg ggaacaaacc
     1501 attttttctt ggtccaagcg agaaatctgt tgattgcttc ttctttctcg acagcaaaag
     1561 gaagtacttt atttggcaag aattctccgc ttaaacgtcc cgatagtacg acgggattat
     1621 ggcagtagta acaataagtc gcagcagttg tcgcatcagt cacgatctct gcaccacagt
     1681 tgggacaaag aaaaagatct atcgctgcgg tattatctgc agattcgtcc gctgtatctg
     1741 aaaaaccgcc tgcctcgtca accgctttgc gctcggcttc attcatatcg gcaagctgtt
     1801 cttgggctgt aaaagtaaaa tcttcggtag gtgaaggttc ttctttgctt tcttcgccag
     1861 ccatacgcgc ttctttttgc ttttgctcat attggctgac ttcttcttct gtatagatat
     1921 tcaggcagta ttcgcaatga aatttttggt ctttgggatc aaaagtcaac ggaccgccgc
     1981 aattaggaca tttatgtgta agaacatcca tcagctgcac acctttctaa ataaattaat
     2041 taacggggat tcctttaaac ggtttaccac agtttggaca gaatttaggg atgccgttgg
     2101 aaagatccac gatctcattg cattcactgc atcgcatttg tagcttaggc tgaacgtttt
     2161 ctacaggttt ggcagctccg cagttggaac agaattttcc ggaattttct gtcccgcaaa
     2221 ctggacatgt ccaagtatcc gcatttcctt gtgcagtttg ttgattttgt ttttctgctt
     2281 gttgtttgat ttgttcttgg ttattttgag ctgcttgaga aagataagaa ccatttgcat
     2341 tcattcccat acctacaccc ataaagccgg tcattgctcc tgcttcattt tttccggctg
     2401 cttccatacc acgtgcaatg gaaccttgca cgtatccttc acgtacgcta ggatcaccta
     2461 gcatcgcccc ttcgttacgc atattgatca gtttcacaga atcatcggtg taagaaatgc
     2521 tggctacagc cacagaaaca atctccatcc cacgtaattc acgccaagaa tcatccaatg
     2581 cagtatccat gtatttgctt aattccaaac tcttagatgg tacataagaa atccgttgac
     2641 catctgcaga catctgattg attgctgatt gtagcgctgt taggaattca gctaaatact
     2701 gctcattgat atcattgatt tccacttgtg ttttgttttt agggattgcg tttgtataaa
     2761 ataaaatcgg gtcaacgaca tgaatagaat aagtgccatg tgcccgtaag aataattctg
     2821 cgttatagaa attgtcaaag tagttcaacg gcgcgcttgt accgaatttg attcccttta
     2881 tttcttggag attgatataa aaaacttgtt gtttctgcgg tgtgacgccg ccaaatttga
     2941 atcggtcaaa agtttcagca attgcttctt tcagtgttcc attgaacatg gaaggagctg
     3001 cgtcattttt aatggtgtaa taaccttcct cagcggtata atcgatgatt tttccaccat
     3061 cgaccaaaag catcatcatg tttggataga catggacgac tgttccgtct gttaaaacat
     3121 cttctgttcc tttacgattg gagccacgtt tgtcatcttt gcggactttg acaccttttg
     3181 tcatgaccgt tgtatcactc atattgtctg gttcgatgac ttctagccat tgatctgcca
     3241 gaccgccgcc aaccatactt gttgctgctt tgataagacc cataaaaaat tcctcctctg
     3301 attcagctag tgccaaacaa ttattgttta tttattatac cataggaaga tgtgaaactt
     3361 ctcggactag agacggaatt gaaatggaaa attgcgtatt gtttaaatac ctgaaaaagc
     3421 cgagaaggat agtagttttt ttacctaata tcacgtataa taagagatag tgaattttta
     3481 aaaaaagggg gatccttatg gcatatcaag cactttatcg agtctggcgt tcgcagcgtt
     3541 tcgaagacat cgttggacaa aaagctgtga cacagacact aaaaaatgct attgtttccc
     3601 acaaaacctc acatgcctac ttgtttacag gacccagagg aaccggaaag acgagtgcag
     3661 caaagatttt cgcaaaagca atcaactgtc cgaacagtaa agacggtgag ccatgcaacg
     3721 aatgcgaaat gtgtcgatca attactgctg gtacgcaaga agatgtgatc gaaatcgatg
     3781 ctgccagcaa taacggagtc gaagaaatcc gatttatccg tgatcgggca aattatgcgc
     3841 ctacaaaggc agcgtacaaa gtgtacatta ttgatgaggt acatatgctt tcaacaggag
     3901 ctttcaatgc cttgttgaaa acattggaag agccaaaaga aagtgtcata ttcatcttag
     3961 caacgaccga acctcacaaa ataccagcta cgatcatttc acggactcag cgatttgatt
     4021 ttaaacggat caatacggca gatattgtcg aacatcttga atttatcttg aaagaatcga
     4081 ctatcagcta tgaaccagct gcattgaatg tgattgcccg ttctgcagaa ggcgggatgc
     4141 gggatgcatt gagtattctt gatcaggcaa tctcgtttag tgaaggaaca attactttag
     4201 atgatgccat gcaagtgaca ggtagcttaa ctgacgaaat gatggacagt tacttgagtg
     4261 cttgcgtgaa aaaagaagta ccagaagcat tggaaacatt gaaccaaatt ctcgcagccg
     4321 gcaaagaatc acgtcgcttt ctagaggata tgttacaata ctgccgtgac ttgctgatgt
     4381 atcagcaagc acctaaccta gttgaggaac aatcgagtac gatcagaagt acatttacag
     4441 aattggcaca aactgtttca gcggatcagc tgttttcaat gataaagatt ttgagcgata
     4501 ctcaaaatga aattcgtttt accaatagtg ccgctattta tttggaagtc gctacagtca
     4561 aacttgcaca gctaccgtct gcttctgttc cttcagcaga tgtagcattt gcagatgatc
     4621 agatagtaaa acagctacaa caagagatta aggaactaag aaacgaaatc aaggaactac
     4681 gcagtaatac aggaattgag aatacaaaag tacagccggt acagcaaaca aatcataaaa
     4741 aacagacaag tacctatcgt gtaccgacgg aacgagtata taaagttctg cgggaagcga
     4801 caagacagca tctgaaccaa gtaaaagaag tatgggaaga cttgctgatg agtctatctg
     4861 tcactcagcg agcaatgctt aaagcaagcg aaccagttgc agctggacct acaggactag
     4921 tgattgcgtt tgattatgag attgtttgtc aaagagcagc aaatgacgaa gaattgcagc
     4981 tttcggtaca caacaactta agccgaatga tcaaagatta tgcgcctgat cctgtttata
     5041 ttacaagaga aagctggcca gaacttcgaa gaaattattt gacgcaggca caagaagagg
     5101 gaacagaaaa ctcttttgct tctgaagact cagaaggaga aatcgagttg cttcctcaag
     5161 aacaagaagt accagaagaa acacttgtag taaaagcaga ggaactgttt ggctccgaag
     5221 ctgttacagt ggtagatgat taataaaaag gagtagataa ttatgatgcg tggaatgggc
     5281 aatatgcaag gcatgatgaa acaagtacaa aaaatgcaaa aagaaatggc agaagcacaa
     5341 gagctgctaa ataaaacaga gtttacaggt aaagccacca atgaattggt caaagcggtt
     5401 tttacaggcg atcgtcgcat gaaagatatc caaatcaatg aagaagtcgt tgatcctgat
     5461 gatatcgaga tgcttcagga tcttgtcgta atggcagtca atgatgcgat cgcacaaatt
     5521 gatgtggaaa ctgagaaaaa attagggaaa tacgcaaaag gtttgccagg attttagttt
     5581 taaactgaag gagtaatgaa tatgcaatat ccagaaccaa ttgcgaaact gatcgacagc
     5641 tacatgaaac ttccaggaat cggtcagaaa acagcgacaa gactcgcctt ttatacgatc
     5701 gatatgaaag atgaagtagt aaatgaattt gctaaatctt tgttaagtgt aaaacgcgat
     5761 ttgcattttt gtagtatctg cggaaacatc acagaagaag atccctgcga aatatgtaga
     5821 aatccttcac gtgatcgaag cgttatccta gtggtcgaag agccgaaaga tgtaatggca
     5881 atggaaaaaa tgcgagaata tcacggactt tatcatgttc tccatggtgt gttatcaccg
     5941 atggaaggga caggtccaga agacatcaat atttcttctc tgttgcagcg gctgcatgat
     6001 gcagatgtaa aagaagtgat tatcgcaaca aatgcaacaa cagagggaga agcaacagct
     6061 atgtatctct cacgtctgat caaacccgca ggtatcaaag tgactcgtct agcacatggt
     6121 ctgtctgtag gctctgatat cgagtatgca gatgaagtta ctttattgaa agctgtcgaa
     6181 ggaagacgtg agttataaat ttctaattaa gtgttttttc agatacaata taaaagcagc
     6241 acaagagatg ggggcaacaa tgtgaacgga ctatttatta caatcgaggg gcctgatggt
     6301 gcaggaaaaa ccagcatctt gaatgaactt taccctcttt tgaaagaagt agcaaaaaca
     6361 gaaatcattc aaacaagaga accaggaggc attccgattg ctgaagagat ccgagcagtg
     6421 atccttgatc caaaaaatga tcggatggat gaacgtacgg aagcactgct ttatgcagca
     6481 gcaagaagac aacatctagt cgaaaaggtt ctgcctgctc tggcaaaagg gaaaatcgtt
     6541 ttatgcgata gatttgtcga cagttctctt gcttatcaag gtgcaggccg acgcatcggc
     6601 gttaccgaga ttgctcggtt gaatgagttt gctacagaag gtactacgcc ggattttacc
     6661 ctttatcttg acgtagattc agacaccgga ttaagaagaa tcaagaaaaa cagacaaaat
     6721 caaatcgacc gtttagattc ggaaggtttg gaattccacc agcgtgtacg tcatgcttac
     6781 ttaaagctgg cagaagagaa tccggaaaga atccacaaag ttgatgcaag aaaaagcttt
     6841 gaagaagtgc tgcaaacaag ttatcataca attattgaac agtatccgca attttttgaa
     6901 aactaggaag gtgaacgttt atgaaaatta ttttagcgat tatccaagac aaagacagca
     6961 accgtttatc aaatgaattg attgatgcaa atatccgtgc cacaaaacta tcatcgacag
     7021 gcggcttcct taaggctgga aacagtacgt ttatcgtggg tattgaagat gaccgtgtag
     7081 aagaagcatt ggaaatcatc aaaaaaacgt gtgaatcaag aaagcaattt gtatctacac
     7141 ctgttacact ggatatttcg atggatgggg gcgtccctta tccagtagaa gtagaagtag
     7201 gcggtgccac agtcttcgtc ttacctgttg aaggattcca tcaattttag gaggagctga
     7261 gctatgaatc aagaaataaa cttggctcaa atgcaaccga tcgtgtatca gcagcttcaa
     7321 cggagttttg agcatgggcg tatcgctcat gcttatcttt ttgaagggga gaaaggaaca
     7381 ggaaaacatg aagtaggtat atggcttgca cagcacctgt tctgtaccca aatgaaagac
     7441 aatctgcctt gtggaaaatg caacaattgt cagcggatcg aaaagaaaga acatccagat
     7501 gttttggtta ttgaaccgga aggacaaacc atcaaagtcg atcagatcag acgtttgcag
     7561 acggaattca gtcgcagcgg ttacgaatca agaaaaaaag tttttttgat caaagaagct
     7621 gaaaaaatga atagcagtgc ggcaaacagt ttgttgaaat tcttggaaga gccccctgga
     7681 gattttttag cggtgttaga aacagatgca gtaggaagaa ttctccctac tattcaatct
     7741 cgttgtcaag ttctgcattt tcaagaactt tcgaaagaag cactgatcca tcgattgcaa
     7801 gaagagcaga ttccattgga aaaagcaaaa cttttggctt tcttaacaaa cagtttagga
     7861 aaagcagttg aaatctctca agatgaatgg tttaatgatg ctaaggactt gatttatcaa
     7921 tggttcgttt acttgcaaaa gcaagatact caagccttta tctatgtcca aaaaaaattg
     7981 gtaaaatcat ttaaagaaaa aacacaacag tttacaggat tatctgtctt gatgttttat
     8041 tatcaggaag cacagcgtca agcagtagca aacggcgaat atggcaaagt gaaacagata
     8101 aaccagataa ttgaacggat attgatcgca gagcagaaac tacgcagcaa tgtcagtttt
     8161 caggctgttg cggaacagtt cgttttgcat acgatttttt agcttgccgc aaaggatgat
     8221 gatcaaggag gatggaaatg gtagaagtag taggtgtccg atatagagat gcaggtcata
     8281 tctattattt tgcaccagga gactctgaat atgtttatag tgacaaggta ttagtcgaat
     8341 ctcagcaatc cttacatatt gctactgttg tccttcctaa aaaagagatt gatcccaatg
     8401 accttcctga agctatccgt ccgatcttgc ataaagcgtc acaagctgaa ttagaaaaag
     8461 acaaaaaaaa caaaaaagat gcttatgatg cattgaaaat cgcaaaagaa aaagtgcagt
     8521 tgcgtgagtt ggaaatgaaa atggtcaaag ccgaatacac atttgatcga aataaattga
     8581 ttttttcttt cacagcggac gggcgaattg actttagaga attggtaaaa gatctggccg
     8641 ctgtatttcg tactcgtatt gagcttcgac aaattggtgt ccgcgatgaa gcaaagataa
     8701 taggcggaat tggtccatgt gggcgtccat tatgctgttc ttctttttta ggcgatttcg
     8761 tgcctgtatc aatcaaaatg gcaaaagatc aaagtttatc tttgaatcca acaaaaattt
     8821 ccggtttatg cggacgcttg atgtgctgtc tgcaatatga gagcgaagaa tacgagacag
     8881 cgaaaaaaga actccctgac tttggaaaag taattgctac acctgatggt aaagggaaag
     8941 tcgtagggtt gaatctgctt agccgtatcg ttagtattcg gctgactgga cgggaaaatc
     9001 ctgtcgaata tgaatgggat gaattgaaag aatttatcca agtaggtgag atgaatggat
     9061 aagagatcat tatacgacgg attgaatcaa ttagaatcag agttgagaaa tacattaaca
     9121 gaacttgttg aaatgaagca tgctttgcat gagatcgtag aaaaaaatac aactttggag
     9181 atggaaaatc aacgtctgcg ggaacacctg cgggaattga accaagttgc acaagctcca
     9241 gctgaaaatg tgaagcagga actttctaag tctcgtatga atcttgaaaa aatttatgaa
     9301 gaaggattcc atgtctgcta cgatttatat ggatcaagaa gggacaatga cgagccttgt
     9361 gcgttttgtc tagaagtgat ctatcgcgaa agcggtcgat gaccaataac aagaggagac
     9421 tggagcggag aaatactgct ccagtctttt tattaaggag attgtgacag atgtataatc
     9481 aaaaaagttt caagggaaat ccggtatatg gggatttata tcttgtaccg acgccaattg
     9541 gaaatttaga agatatgagc tacagaagtg ttcgtatgct gcaagaatcc gatcttatcg
     9601 caagtgagga tacgcgtaat acacaaaagt tattgaatca ttttgaaatc catacacccc
     9661 aaaaaagttt acatgaacat aattataaag aacgtgtccc tcaattgatt gctttgttaa
     9721 aagaaggaaa aacgattgct caagtcagtg acgcaggaat gccttccatc agcgatcctg
     9781 gacatgagct agttttagcc tgcataaaag aagggatttc tgtgatctct atcccaggac
     9841 ctacagctgg gatgacggct ttgattgctt cagggctagt gcctcaacca tttttatttt
     9901 atggcttcct tccacgtaag aaaaaagaac agaaagaaac attgaaaaag ctaaaagaac
     9961 agacagttac gctgattttt tatgagtcac cttatcggat cactgctaca ttgacgaatt
    10021 tcttagaaat ttttggaaat cgccaagtcg ttttgtgtcg ggaattgaca aaggtccatg
    10081 aagaatacct tagaggaagt acggaagagc tactcgctta tatcgaagaa catccagtaa
    10141 agggtgaatg ttgtctgctt gtagaaggga atttgttcga aatgccagtg acggaagttg
    10201 atgaagagtt gggaacgtta aaggaacaag tagaagcaaa gatcgcttca ggcgaaaaac
    10261 caaatgctgc tatcaaagca gtagcgctga aaaatgggct gaaaaaacaa gaagtctacc
    10321 gtcagtacca tgaattagac tgagatagta taagttgtct ataatgaaag actaatgagg
    10381 aaatcagcta ctaacttttc aaatctcttg aacgcgaacg aatgttcttg tataatgaat
    10441 aagaggtgag gatgatggag ctgcaatttc ctttacataa tgatacatca agaaaaatca
    10501 ttcatattga tatggatgct tttttcgctt cggttgaaga acgagatcat cctgagtttc
    10561 gcggacatcc attagtaatt gccagacacc ccagtgacac tggtgggcgt ggagtagtga
    10621 ctacagcaaa ttatgaagca agaaaattcg gaattcattc agcaatgagt gcacaaaaag
    10681 cgtatgagct ttgtccgcaa gccatcttta agcctggcga ccatcataaa tacagcgata
    10741 tttccaaaca agtacgggaa gtattctatc gttatacaga tattgtggaa ccagttagta
    10801 tcgatgaagc gtatcttgat gtgaccgaaa ataaaatcaa ttgcaagtca gccatcaaaa
    10861 tcgcccgaat gatccaagca gatatttggg agagtgtcca gctaacatgt tctgcgggag
    10921 taagttacaa taagtttttg gccaaattag catcagacta tcaaaaacct agaggaatca
    10981 cggtagttgc ccctcaagac gctgtgccat ttttaaaagc tttgccaatt gaaaaatttc
    11041 atggggtagg gaagaaaact gtacctagaa tgcatgagct agggatttat acaggaaaag
    11101 acctatatga atgcacagaa atgatgttga ttcgaaattt tggaaaaatg ggctattctc
    11161 tttatcgtaa agtcagagga atccatgatt ctccagtaaa cgttacaaga gagcgaaaat
    11221 ctgtgggaaa agagcatact tatgggcagc cgttgcaaac ggaagaagca gtgctgactc
    11281 agttgcgcca actagctgaa aaagtagaag aagcactgag aagagtccaa aaacatggga
    11341 aaacagtagt tttgaaggtc aggtacacgg actattctac tgtgaccaaa agagtgacat
    11401 tgccagaata tatctataaa aaagaagcac tcttttatca agcaagcctt atatgggagg
    11461 aaatcttggg agtagaaaaa ggaatccgat tgttagggat tactttgacg aatctggacc
    11521 cgatgactta tgaaaacatc gtccttccat tatgggaaaa tcaagaaata taaaaacagc
    11581 tttgagagtt gaaaccggaa atccgttttc tctcccaaag ctgtttttat atcaagtctt
    11641 gctttttgat ttgttcatag gtttctgttg catccgtaca tttttcccaa atcacaactt
    11701 gattggattc taaagatttt tggacgtcaa tgatccgctg gttgctactt ccgcgaaatt
    11761 gtagttttag gtcacgtttg cttaattcaa agcgtccgtc aacaaggata tcgatttgcg
    11821 aaaggagttc taatttatct tctgaatctt gtagcaattc atcaaaggta tagcctgtcc
    11881 atgaccagat atcttttgtc gtaccgaact cttttcggat acgtcgtacg acttgcagac
    11941 aaacatctgt attcaaaaaa ggttcgccac caagtaaagt taatccttgc acgtaatcgt
    12001 tgcgcaagtc agcgatgatt tgttcttcta attcaggtgt gaatggtttg ccatatcgga
    12061 aattttgtgc agctttgttg aagcagcctt cgcaggcaaa taagcagcca ctaacgtaaa
    12121 gactgttgcg gacaccttca ccatcgacaa aattaaacgg tttgtagtca gcaatatagt
    12181 tttggctgta gtcttttgct aaccattctt ttggttttgg atttctcata aaaaaacttc
    12241 tttcatttaa ggttcaaaga tatagctatt gaagataaaa tctactcgac cacataaata
    12301 agaacaaact gacctaaatc cattgttcaa gaaagcttgt tcttattttg atagagggcg
    12361 ttcaaagata caaagccatc gaaagagggt ggtgaaaaaa gacttgtcat agcctcttat
    12421 tctgaataaa cggtgttcaa aagcttgcat ctgcagtaat aagcccaact aaacgaaaaa
    12481 tatgaggaac tatttttgtt tagctattct tattacttga tgcaggacag ctttttcaca
    12541 acctcatttc atgtgtttca cacgtgagga aatttcttta tggcggccat gtaccatcgg
    12601 acgagcttgc ggatttccta aatagccaca agtacgtttg actacatcac atgtttttgg
    12661 atcatgattt ccacattctg gacattcgaa ccctcgttct gtcggtttga aatcaccttc
    12721 gaaaccacat tgatagcaat gatcgattgg cgtattcgta ccaagatacc cgattttgtc
    12781 atatgcaaaa tcccatacag cttctaaagc tttcggattt tgttggagca tagggtattc
    12841 acagtagtgg atgaatccac ctgaacaata ttttggataa tccttctcaa attccaattt
    12901 ttcaaatggc gttgggtttt tacgcacatc atagtggaaa ctgtttgtat aatattcttt
    12961 atctgtgata ttttcgatga tgccgaattt ttctgtatcc aaacggcaga aacggtcagt
    13021 tagactttca cttggagttg aatagacact gaaatgataa ccatattcat ccccccatgc
    13081 atctgcatta gcttttaatt ctttgacgat atcaagtgtg aaagcttttg cttctgggtt
    13141 tgtttcccat tcgccaccga agaaagctga agcaacttca tacaatccaa tgtagcctaa
    13201 cgatacagta gctcgacggt ttttgaataa ttcattgaca gaatcggtac gtgaaagacg
    13261 tttcccgaaa gcaccataca tatataaaat tggcgcattt gctggaatgg cttctttaca
    13321 acgttctaca cgatagagaa gcgcatcttt catgatttct aagcggtcag acagtaactg
    13381 ccagaattta ttctgatctc cttttgattc gatcgcgatt cttggcaaat tcaatgtaac
    13441 aacacctaaa ttcattcgtc cgacatttac ttcttgacca ttttcgtctt tccacccttg
    13501 caagaatgaa cgacagccca taggtacttt gaaacttcca gttaattcaa cgattttatc
    13561 gtaattcaga atatcaggat acattctttt cgttgcacat tccaatgcta gttgtttgat
    13621 gtcgtaatta ggatcttcag ggtttaagtt cacacctttt ttcaaagtga agatcagttt
    13681 agggaagata gctgtccgct tttcactccc taatccattg attcgaatct gtaaaattgc
    13741 tttttgaatt tctctttcaa accagttttc tcctagtcca aatcctaatg aagtgaaagg
    13801 agtttgtcca tttgaagtaa ataaggtatt gatttcatat tccaaacttt gcatagcatc
    13861 gaagatatct tttttggttt ttgcttttgc atattctttt tggcgttctg gcgaatcgat
    13921 ccattcttcc gcgtctttta gatgtttttt atagttcaat tctgcaaaag gagcaagcaa
    13981 ttcatctgtt cggtcggcag aacagccacc atattgactt gacgctacat tggcaataat
    14041 ctgtgagatt tgtgctgtgg cggtctggat agattttggc gattcgactt cggcattgcc
    14101 gattttgaac ccattgttca gcatgccttt gaaatcaatc aagcagcaat tcgtcatcgg
    14161 tgtataagga tgataatcca aatcatggta atggatatcg cctttttgat gcgcatttgc
    14221 tatatgtggt ggcaacattt tcagtccgat tgattttcca acgatcccgg ctgtcagatc
    14281 tcgctgtgta ttgaaaacat tactgtcttt atttgcgttt tcgttcacga cagtttggtc
    14341 cttgttgatt agcttttcaa tcgaaaagtt gatatctgtt gctttactgc gagcaaaatc
    14401 tcgtttggta cgataattga tgtactcttc tgctaattca tattcgtttt tttctagtaa
    14461 tatatgttcc acgatgtttt gaatctcata gattttgaca ttttcagcaa accggctttt
    14521 gatttcttgg acaaccaagt ctgcaatcat ttgaattttt tcataagtaa gtggtgataa
    14581 tgagccgtga attttctttt ctgctttgat cagtgcttca ttgatctttt gttcatcgaa
    14641 atcaacatgg cggccatcgc gtttgatgac tttcatctgg gatgtctctg gctgctgagt
    14701 aacgacagtt ttttcttcat aatttacttt cattatcttc aactcctgct aaattgttct
    14761 tgtctatcat aaaacaaaat cggggaagga gcaactaaat atcggctata tatagtattc
    14821 aatgtgctgt ataattggat ttctatacta tatgtagtgt atattttatt gtgaaaaaag
    14881 gtgaaaactt gtagctggtg gatttgtttt ttgcatattt aattttgctg aataaaataa
    14941 ttgaataaaa ttttttattc agtccaagta aaagtgacaa aaacataaga gcaaattatt
    15001 cgtagatatg ctactatgca aataaataga aatgaaaaat gaaagaggag gagaagaatt
    15061 ggaaaagatg atcgaagtga aacatctaag taaaagctac ggcagcaaac tttctcccgt
    15121 caaagcctta gataatatct cattcacagt agaagaaggc gagtttgttg ggattatggg
    15181 cccaagtggt gcaggaaaga caacgctgat gaatatcttg tccacgatca atttgcctac
    15241 gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa atgaaaaaca gtgagctcag
    15301 tgactttcgt cgaaaaaaac taggattcat ttttcaagag ttcaatttga tcgataccct
    15361 aaatgcaaaa gacaatatcc tattgcccct agcagttgaa cggatgacta aagaggaaat
    15421 ggaaaagaga gtaagacatg tagctcaatt gttgaatata gaagaactgc tcaaacggta
    15481 tcccgatgaa ctttctgtcg gacagagaca acgaattgca gctgctagag cacttgttgt
    15541 tcagccacaa gtgatttttg cagatgaacc aactggctct ttggactcga agtcggctac
    15601 agaattgttg aactacttga agaccatgaa tcaaaaagaa aaagcaacga tactgttagt
    15661 cacccatgat ccatatacag caagctactg cgatagaatt ttatttatta aagatggtgt
    15721 gattttttct gaagttgtca gaagagggac aagaagagag ttctttgaga aagtaataga
    15781 catgcaggcg acgattggcg gaggaggaaa aatgaatgct gtataaacta gccgttaaaa
    15841 gtttttttcg gcagtcacga ggctatcttg tttatttttt cagtttgact ttatccgtaa
    15901 tgatctatta ttccttcagc gcaatgacct acgatcaatc tttagtcaga agagcaagtc
    15961 aagatacttc gatagatggt ggattgagtt ttggtggatt tatcattact gttgttttgc
    16021 tttttttcgt attcagtgcc aaccgatttt tcttgaatag aaggcaaaag gaaataggca
    16081 tctatcagct ttttggtatg aataaactgc agatatcagg tatatatgtg ttagaaatca
    16141 tgataattgg attgttcgcc tgtatttctg ggatattact cggtattatt ttttccaaac
    16201 tcttttcaat gattttggtt cgaatgatgg atatggatct tactagtcca ttttttatct
    16261 ccattccatc agtagtagat actttatttg cttttttcat catcctttta gcagtatctg
    16321 tatattcaat atggaaaata tggcgctatc caatgattcg aagttttgga gaaaaagaac
    16381 aagcagatag ttcaacaatg cgcttcagga caaggcaccg attactcgca gtcatcgggt
    16441 tactactttt aacgagcgga tattttggag ctagtcattt tagggaaatc acttcatgga
    16501 tgatcagtta tcattatctt gatacattag ttttcttttt tcctttttta atttttttcg
    16561 tttgtgtggt cggtacttat ttgtttttct gttatacatt tcggttattt ttgatcatat
    16621 tgagtaaatg gaagatttat tactatggta tcaatttttt gataatgggt aacacgcaag
    16681 ttcatttgct caaaagttgg cgtacaaatt ctctgattac agtcattctc ggaatttctt
    16741 taatgacaat tggcggcacg gctagtatct ctactatttt atcctaccag gagaaagttt
    16801 cctccccgat ggactatcag tttgatgttg agacagagaa aaaaatcaag ccgattctag
    16861 cagaggaagg gcagaagata actaaagaaa tcaccttgca ttataaagtc attgggagta
    16921 tctatagtca aaatctcgaa gaacctatgt ttcaaactgc taatctgatc acggaaacac
    16981 aatatcaagc cttcagaaaa gttaatccag atttgccgaa catttctttg aaatcacctg
    17041 gtcatactgt tttgcttgat caaatgcaaa ctctttttcg gaatatttca ttatatggat
    17101 ctagtattgg actgccagat aatcaagaat tgaagattca acaaatgctt ccaagtgtat
    17161 taggtgacga aacaatggta tatctaagtc ctgtactgat tgtagatgat accgtttttg
    17221 aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga tgtatcaggt ggaaataatg
    17281 aaaaaataga tgaacgactg agcaatgacc tgtcgataaa gtggctcaat gctgtttatt
    17341 attcttatga tatcgtaaat ggaagtctac aaggagaaat tagcacaaag aaacttccgg
    17401 atgaccaaat gaaggaagca caatttagcc aagaaagctc tcgtttgaat ttctcttcca
    17461 gctattcgaa attgcgtgca gttaatcggc gaatcggtat caatattttt gtcacactat
    17521 ttgtcgggat gatcgtgatt attgctactg gtagtatttt gacagtccgg caattagcag
    17581 aagcagagga agaaaaagaa aattatcacc tattgactaa gctggggatt ccacaaagaa
    17641 ggatcagacg gatgatcttc gagcaaaacg cacttacctt ctttccaccc atgatattag
    17701 ggatcactca cgcagtattt gcaatcaatg tcttcactca gtatataaaa actgccgatt
    17761 actggttggc ttatcttgtg agtggcttat tgatccttat ctatcttttg ctttatgtca
    17821 ttacgagtca gttatattgt cggattatcg aggaatgata tacggaataa gacaagataa
    17881 taaatagaac taaattttga ataccattta tttggaataa gaggttgtga cacgattttt
    17941 gtcacaacct ctttattttt tatatttttt aaaagtacga gaaattataa agaaaaaagc
    18001 agtgatttga tgtgctattt ttataaaagc tatgtgacaa aactgttagt aaaaaaacgg
    18061 tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt
    18121 atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa ttattgggga
    18181 aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat ttatctgtat
    18241 cactataaaa atattcaagg aggaactttt atctataacg aattttagga ggaagagtat
    18301 ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac ctgatcgagt
    18361 tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat cagacgcact
    18421 tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt ttggcaattt
    18481 ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg cctatttgcc
    18541 gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc
    18601 tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg tcataacaaa
    18661 aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa atagcgtaaa
    18721 aggtacagaa aatttttata ttatttttac ttctggtact actggagaac caaaaggcgt
    18781 acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag actttgggat
    18841 caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat cggtattttc
    18901 tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag cagttgttca
    18961 agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt gggtttctac
    19021 tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa atgtgccgga
    19081 attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag aaagtctact
    19141 tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag caactgtagc
    19201 tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac taccgattgg
    19261 ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg
    19321 agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc
    19381 cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc atacagggga
    19441 tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg atcagcaggt
    19501 caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc tccaagacaa
    19561 ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac agcagctggt
    19621 tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga ctaaatctat
    19681 caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt tccattatgt
    19741 cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt
    19801 gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta tttttttctt
    19861 ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa aagattacca
    19921 gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg taattattgg
    19981 caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt atggggctat
    20041 tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt tattttagcg
    20101 ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa agcatcgtta
    20161 cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat catggaaata
    20221 agagatggca tgatcaaaac atataagcca tttcgttata tccagtttct gctgttcttt
    20281 ccaacaatct cttctggacc aatcgatcgc tataggagat ttgaaaaaga tcagctccat
    20341 ccgcctgttc gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc
    20401 ggttttttat ataaattcat tatcggttat tattttggtc aagtactgct tcctgtagtg
    20461 ggacgcattg ccttacagca tggtggtatt tcatgggcat tagtaggtta tatgtacgtg
    20521 tacagtatgt atttgttttt tgattttgct ggctatagct tatttgcagt cggtacaagc
    20581 tacatgatgg gatatcagac accaatcaat ttcaataaac cttttttatc atggaatatc
    20641 aaagaattct ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga ttatgtatat
    20701 atgcgattga tgttcttcat gatgaagaaa aaatttttta aaagcaagat cgtaatgtca
    20761 aatatcggtt attttgcttt gtttcttcta atgggtatat ggcatggatt gacgtggtac
    20821 tacatcgtat atggactgta tcatgcttgt ctgatctgtc tgacagatgc atggatccga
    20881 tttaagaaga agcataaaga gcagttgcct tctaacaaat ttacgcatgg cttagccgta
    20941 tttttgacat ttcaagcagt atgttttagc ttcctgattt tttcaggatt tttagataaa
    21001 ctttttttta gctaacgaat agtgacatat taatttatat aacaaaggag aaaaggaaaa
    21061 tggaagaaca aattttatca atcttagaag aaataacagg aacagatgaa gtaagaaaaa
    21121 atcgtgatat tgatctattt gatgaagggt tgcttgattc tttagcatct gttcaattat
    21181 tagtcgagct agacggagag ctggatatcc aagtccctgt atctgaattc gagcgagagg
    21241 attggagcac accgaataaa atcattcaac aagcaactgc attgaagggt tgagtatgaa
    21301 aaagaaactt tttgctattt tcggcccggt tgttttagct gttggcttac tgtttgtgtt
    21361 ttttacctcc tcctatcaag tagatatctc taatccagaa acgattagac gtgcctcttc
    21421 ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa
    21481 acaatatatt cctttttttg gctcaagtga attaagcaga atcagtccat ttcatccgtc
    21541 tgttctggca gaaaaatacc agcgaaacta tcgtccattt ttgctgggag cacccgggac
    21601 acaatcgtta agccaatata tgatgatgcg ttctgcagga gatgctatga aaaacaagaa
    21661 agtcgtcttt attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt
    21721 taatacgtat tattctgaac ttcaaacata cgactggttg ttttcaatga agaaagtaac
    21781 gcctgctgat cgctatttag ctagaagatt attaactttc tcaaaagtaa aagagaatga
    21841 tacgttgacg gcgattctgc aaacgatcaa aaaagggaag ctacctttac ctgaatcttt
    21901 gaatcagctg cgcagccaat ggaacatgct gaagagagag gatgaagtat tcagcaatat
    21961 tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca aaacgattac caaaacaata
    22021 ccaagaaaca gaattatcca ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa
    22081 tccattcggg ttgaaaaacg atttttatac gcatcgtatc cgagcccacg agccagagct
    22141 caaacagtca caaaaaaatt gggattatcg tttttcgcca gaattttctg attttcagct
    22201 ggtcttggat caattagcaa aaaaccataa cgaagtattg ttcatcatcc caccggttaa
    22261 cgagaaatgg agcgattata ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa
    22321 gatcaaattt caattaaata gccaaggatt caatcgaata gctgattttg tcaatcaagc
    22381 gggtaccaac tattttatgg aagacacgat ccatctaggc tggaaaggct ggttggctgc
    22441 agatcaacag atcagaccgt ttttagaaga aaatcatatt actgctagta agtatcatct
    22501 tgacgatgct ttttttagca aatcttggca acatcaaata ccagataaac tacaattaaa
    22561 ataaagaaaa caggcgagtg ttgctcgctt gttttcttta tttttgaata ttctgaataa
    22621 aagataaaaa taccaagttt gcctgaacaa aaatcataag caatggtata atcacaatat
    22681 gaatatagaa atgggggaaa acatttggat acaaaagaag aacagctact tattgattta
    22741 acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt cagagaatat
    22801 gccaaaccat tcgctgatga tattttttat gacggtttag gcagtgtgat tgccaaacat
    22861 ggtgctggcg gaggccccaa ggtctttatt tctggacata tggacgaagt tggttttatg
    22921 gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa ctcttggagg ttggtggggg
    22981 caagtcatgc ttgctcagca agttgaaatc aaaacacgtg aagggaaaat cgtccacggt
    23041 gtgattggtt ccaaaccacc acatgtttta acacctcaag taagaaataa accttatgaa
    23101 ataaaggata tgttcattga tattggcgca tcaagccaag aagaagccaa agagtggggc
    23161 atacgtcctg gagatatggt cactccatat attgaataca aacgaatgaa tggcagtaaa
    23221 tatttactgg caaaagcatg ggataatcgt atcgggacag ctgtttctct gcgagtatta
    23281 gagaatctat ctaaagaagc acacccaaat gttttattcg caggaagcga tgtgcaagaa
    23341 gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg taaatcccga tattgctttt
    23401 gctttagata ctggtacagc aggagacact ccaggaatga caccaaaaga agcagattcg
    23461 attttaggaa aaggcccaca gattttgatt tttgatgcat ctatgatccc gcataaaaaa
    23521 ttattgaatt ttgttattga tgtagcagag gaaatggata ttccattcca atatacagtg
    23581 atttctggcg gaggaactga tgcaggacaa atgcatttga caagagatgg tgttccgtct
    23641 gtagccatta ccgttcctgt tcgttatctg cattcccata catcagtgat tcatgaagat
    23701 gattatttga atacagtgaa actagtcaca gaattagtga aacggcttga tagtgaaaca
    23761 gttgaaaaaa ttcggagtta tgaataatga ataatagaat acctatttct gaatatcgga
    23821 aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa tacttggctt gttttcttat
    23881 ctataatatt tacgagcgtc atccagaact tttttgacgg tttcagtcct tttccggaag
    23941 aaagcatgca gcagcactta ttgagttttc tagtcgataa tttactttta ttcgtattca
    24001 actatgcatt gttttacatt gcgctagtag ttgtacgggg tggcagacca aaaagtaacc
    24061 ttttattttc agttttccaa agaaagtatt ttggttcgat acttggcatc aatttgatca
    24121 attcagtcat caactggcta ctcaacagtt taatctttct gcccatgttt ttaatcagcg
    24181 gagtgagtac ttatatgcag ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc
    24241 cagaagattt tcttgatatc agctttgttt tatcgatgat agggatgatt tttgtttcag
    24301 cactcatcat gagtattgtt ggcggtttat tccaatttgc tgcatggaca aagttcgact
    24361 atcctgattt gtcggttatt caatgtttga aatatggctg gtttttattg aaagatagaa
    24421 ttggtacgta tattttgttg cagctgtctt ttatcggctg gtatttgcta ggagcaatcg
    24481 ctttgttcat cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt
    24541 atgaacaagc acggattgaa aaaggaggtc cagacgccta ttttacttag catgtaagca
    24601 gtttattcat caaaaaaata acttttgtgg taggataaat ctttgagggg gaatgtacga
    24661 tgaaattaac tgttttagga tgcttaggag cgtatcctta taaaaatcaa gggacaacag
    24721 gttatttgct tcaatctgac gggttcaatt tgttgatcga tgcaggtagt gctacgctga
    24781 tcaaattaca agaacacctt gatccacttg atctagatgc tgtgatcctt agccattatc
    24841 atcatgatca tattgctgac cttggtgtat tacagtatta ctggcaactt catcctaaga
    24901 gaaatccgga gacattgctt tcaatttatg gacacacaaa agattccatt cactttgaag
    24961 aattgacgtt ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa gaattaaaaa
    25021 ttggtccttt tctgattact tttatggaaa caatccatcc agtagtatgt tacgccatgc
    25081 gtatcgtaga agaaacaacg gggaaagtat tcgtatttac aggtgattca ggttatttgg
    25141 aaagtttcgc tgattttgcg aaagatgctg atttgtttct agcagatacc tatctttttg
    25201 aaggaaatga acatcatcat gcccatttca cttcaaaaga agctggagaa cttgcgaaat
    25261 cagctaacgt aaagaaactt gtacttacac acctacctca gtttggtgat ttagaacaat
    25321 tgaaaaatga agcgcaaaag gcagcaggca aagggattga tatagaactt gctgcagttg
    25381 acaaagtgtt taacatttag gaggaagaac atgatttttg taccaaatga aaaccacgac
    25441 ccaagaatca atttagcaat tgaacaatat ttgctgcaag aaatgccttt agatgaacct
    25501 attttgctct tttatatcaa tgaaccttca atcatcatcg gcagaaacca aaatacgctt
    25561 gaagaaatca atcgagatta tgtggaagaa aatggcattc atgttgtccg tcgcttaagt
    25621 ggaggcggag ctgtctacca tgattttgga aacctgaact ttagttttat catgcctgat
    25681 gatggggatt cattccgtga ctttgcgaaa gtaacacagc caatcatcca agcacttcat
    25741 gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg atctagtcat tgatggcatg
    25801 aaattttctg gcaacgcgat gtatgctacc aacggtcgga tgtttgctca tggaacaatt
    25861 atgttcgact ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc
    25921 gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga atatcaaacc atttcttcca
    25981 gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc
    26041 ggcgtagagt cagtagaaga agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa
    26101 atcaatcgaa tctctgagga atattatcga aattgggatt ggaattacgg gaagtctcca
    26161 gatttcaatt tctctcgcca aaaacgtttt tctatcggtt cgattgaagt ccatttgaat
    26221 gtctcagaag gaatgatcaa agaagcaaaa atctttggcg atttcttcgg actaggagat
    26281 atctctgacg tggaaaaaaa attggttgga cagaaatatg ataaagcatc tctatcaaaa
    26341 atagtcagcc aaattgatgt gaagaaatat tttggtgcga tagaaccaga agaattgctt
    26401 accttacttt attgaaaata aaaaatagcg gaatccacat atatggattc cgctattttt
    26461 tatacaggat tcgggtaatt tcgagagagt tgaacagtga tccgatcaat gaaagtatct
    26521 ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat cattaagtca
    26581 aaacgttcct gatcatctaa ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa
    26641 tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa
    26701 atttttcgat acagtggatt ttcgatggca tgagtttctc taaaggaatg ctctaaagcg
    26761 tggatcaaaa gttgagccag atcgtcatcg ttgataggaa gttcttcggg aagatctgct
    26821 tggaaggtta tctgaatatt ccgtttggca gctaacttct ggtaacgcgc taagataaga
    26881 tttgtcacct ggtggttgca aaaagaacac agcatttcga aattttttgc ttcttctaat
    26941 atcttttcca catagttttt agcttttgtt tggtccgtat gcattaaatc ttgcagattc
    27001 tccatctctt ggacaaattc caggcgaagt gtatttactt cttggatatg ctgtcgaagc
    27061 tgctcataat gttgctccaa caaatcattt ttgattttta agaaatctac ttgtctccga
    27121 tctttgtcgg tatttaagat gtgatcgatt agattgtagc tgataataac gattagaatg
    27181 ataatatata aaatcgtaga ccaattgatc gttgtttgat agatacctaa tgccgtttgg
    27241 atgtaaatca agcaatggat aatggctacc aagacgatcc aaggtgtaca agctacataa
    27301 aaacggttat tcttatatgc ttcgccaatg catgcaagtg aagtgactaa agtgcttata
    27361 acactagcaa tattagcaat cggcattaat tcaggaagtt ctgcaggagt aaaagttgca
    27421 taaagcagaa cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt
    27481 ttatagtaaa acattcggct ccaatagtag cagatgatga aagctgacgt taagataata
    27541 aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa gaataccact taaaatatct
    27601 tctgatacag cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat aagtgagtat
    27661 tcaagacgtt tttgtttata catcaagtag atcgtataga aaaaggtccc tatagcgata
    27721 aaaacagcga ttatcatcgt caatacttgc ggcactgatt gtgaataaat aaaactaatc
    27781 aatggtccag ttgttcctag ataaactttc ggtggcagtc cagcatagtt ctgataagga
    27841 gatgagactg taagggtcaa tttttttccg atataatttg ctggtaaatc tatcacagcc
    27901 aatgagagtc cgggattgct ttcttcctga taatctttcc gttggtataa aatcttttcg
    27961 cctagatata cagtaatcca ttgatggttt cctttgatta gtaaatttgc ttcgtccaac
    28021 ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt tatccacggt aggaatcgta
    28081 cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa aacccacgaa
    28141 tcttttattt ctttcatttg attgctgtcc gcaggagaaa aaataaaata aaagaaataa
    28201 gcaattcctg ttaaaaataa caatgataat ataatgatta tgctccactt tatacgtaaa
    28261 taaaaagatt cttttttatt atatatgtta ttaaaaaaag acataaaggt attcctttct
    28321 aaaacttgta atgttagggt aaacgttttt taaactaacg tcaattattt ataataaaaa
    28381 atgagataaa gatgaggaaa tagaattaga aacatataat tattaaaggg aaacattttt
    28441 acagctataa tttgaaaaaa atttatcgtt attatatgat taagaaggat tgaataaaga
    28501 aagaagttga aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg
    28561 attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact
    28621 ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat
    28681 gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat
    28741 tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt
    28801 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
    28861 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
    28921 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
    28981 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
    29041 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
    29101 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
    29161 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
    29221 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
    29281 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
    29341 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
    29401 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
    29461 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
    29521 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
    29581 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
    29641 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
    29701 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
    29761 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
    29821 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
    29881 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
    29941 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
    30001 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
    30061 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
    30121 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
    30181 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
    30241 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
    30301 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
    30361 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
    30421 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
    30481 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
    30541 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
    30601 ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt
    30661 atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt
    30721 ggcattgctt tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac
    30781 tttgtgaaaa atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga
    30841 aaatttcatg aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa
    30901 gaattttcag gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta
    30961 gctgctgtag cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc
    31021 gcaggtttga ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt
    31081 tatcaaatat caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca
    31141 aagggaatgc aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca
    31201 ggacttttcc gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt
    31261 acttcaggta ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac
    31321 tcggaaggta caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata
    31381 aacacagcct ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa
    31441 aaatgggcga aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg
    31501 ctattgttcc atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt
    31561 aatcgtttga atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct
    31621 attagcattg ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga
    31681 atggcaaata agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg
    31741 ttgcttcctt tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc
    31801 atcaaatctg gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc
    31861 tccatgcttg tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt
    31921 ttgattgtga cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat
    31981 aaatattggt cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat
    32041 ttaagtattg ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc
    32101 gctgctatca cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg
    32161 gaaaagctga ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca
    32221 gaaaaactca aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg
    32281 cgaggcgtac caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa
    32341 gcagagcaaa gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa
    32401 ctttctcact tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg
    32461 acagctttac atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca
    32521 agactgtaaa ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg
    32581 aagctagtac ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag
    32641 ctgctggatg aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct
    32701 tttgttctgt taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg
    32761 tatattcttt gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg
    32821 gctggacctt catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag
    32881 taggtgtccg atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag
    32941 tcacgcgata cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc
    33001 cagttgaaga aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg
    33061 tatcaatctc tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc
    33121 cttgcccagc gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta
    33181 tcagttcttt tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag
    33241 aaacacaatg cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc
    33301 ttgaattttt gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt
    33361 aagctagtac tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag
    33421 cacagcatat gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat
    33481 agcatgtacg caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca
    33541 atctatgttg aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc
    33601 ttagtaggcg gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa
    33661 ggtactgttg aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa
    33721 aaacattcac accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg
    33781 atcaacgctt aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc
    33841 gaaatgacgc aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt
    33901 atggaagtga tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga
    33961 ttgcctcatt agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc
    34021 tttataccga agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat
    34081 tagaaaatct tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa
    34141 ttaaaacaat taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat
    34201 tatttgccca caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac
    34261 gtctaggtgc taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc
    34321 aacgcggtac aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat
    34381 ttgcaaaagt tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt
    34441 ctgtttatcc agtagctcaa gaagcttaat tgaatcttat gattaaagag gttgtgacaa
    34501 gacttttgtc acagcctttt ttgtttataa aaataaaagt tgtagtggag gctacgaagt
    34561 ttactttgtc tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata
    34621 actatcgttt tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt
    34681 aaacagtgtt ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt
    34741 aaattgaaca tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg
    34801 acgaaacaga aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga
    34861 aaaggagcga taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa
    34921 accgctcatt taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa
    34981 gtgttaggga ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga
    35041 aaactgataa agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac
    35101 gtaaagccag ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa
    35161 aatacactcc aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc
    35221 atgaaaaatt cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat
    35281 tgtttgatta caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat
    35341 tttgcaaaag cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct
    35401 gattatgcca ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct
    35461 tttacaggcg cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg
    35521 tttttggatg agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg
    35581 gatcatggaa cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg
    35641 atcgtaggtg cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt
    35701 atcccaatca atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat
    35761 ttagtaaaaa tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca
    35821 gaagatgtag caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag
    35881 tcaaatatcc agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc
    35941 tcgatcacaa tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc
    36001 aaccgcagtg caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca
    36061 aatgatacga tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat
    36121 atcatcggag ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat
    36181 catttcattt ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt
    36241 tcttttaatg cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc
    36301 aatcagattt atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt
    36361 tatgctatga gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg
    36421 catacgaatg ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg
    36481 aaagaagtgc gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac
    36541 tattacttga ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt
    36601 gtagtcgttg cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag
    36661 ttgttagatg cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca
    36721 gcattggacc gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc
    36781 ttagtagata tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt
    36841 ccagtacgta ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc
    36901 tcagtattag ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac
    36961 gcagaaatat cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct
    37021 gttttggcta tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa
    37081 cgagcggtaa acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa
    37141 gtgaaagaag aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt
    37201 actgatttga atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc
    37261 gaagtcattt tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc
    37321 ctagatgaag agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt
    37381 attaatggta gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg
    37441 acgggaatcg gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga
    37501 atgatcgaac gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat
    37561 acaacgaatg atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag
    37621 ataaaagtaa aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag
    37681 ttagataaaa aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt
    37741 atcgtttttg tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt
    37801 ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata
    37861 ggggtttctg tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg
    37921 tatagatgat cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt
    37981 tgaacgtatc attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact
    38041 aaaagaagct gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca
    38101 aatctatcat agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca
    38161 agatgttgaa gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat
    38221 gagttttact gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat
    38281 tgattccttt cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc
    38341 tgacaggaaa atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg
    38401 gttgttggaa aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa
    38461 tttgttacaa caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca
    38521 tggacaattt gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa
    38581 cgtcaaagcc gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga
    38641 agaagcggtt gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg
    38701 cggaacaccg ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat
    38761 cgtcagcggt ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga
    38821 atctgctcat gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa
    38881 accggaagaa ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa
    38941 aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg
    39001 tgttgactca cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc
    39061 aaaccgtatt atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat
    39121 cgaacaagct gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga
    39181 agtagcaaaa gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga
    39241 agacgtattg acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat
    39301 ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt
    39361 tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa
    39421 tgattcacgc gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc
    39481 gtaataaata atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta
    39541 gtcgtattag ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat
    39601 caaccactag tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc
    39661 gtacttggcg gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta
    39721 gcaccagatg ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa
    39781 ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg
    39841 ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct
    39901 gctgaagaag gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa
    39961 ggggttcgta tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa
    40021 tcattccttg aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg
    40081 gtcgtagctg taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca
    40141 ttcttcgtta ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt
    40201 gctttaggtg ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca
    40261 aataatggag gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa
    40321 tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt
    40381 tagctgttgc gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa
    40441 acggtggctg ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag
    40501 aacgttctgc agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt
    40561 ctccaatttt aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg
    40621 atgatgtagc gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg
    40681 acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta
    40741 tgggaggaaa tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg
    40801 cattcatgtg gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc
    40861 tttctggcgg attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat
    40921 tagctgccct tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa
    40981 aattagataa aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca
    41041 agagtgcatt tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat
    41101 tacaagacaa cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat
    41161 gtatgtggtt gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag
    41221 tcggtgttgt tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt
    41281 gtcccgagct ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg
    41341 gttcaatcaa tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg
    41401 acagattacg ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat
    41461 agacgcaaat atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg
    41521 ctaaagggaa aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca
    41581 ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat
    41641 agaatggtac aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt
    41701 aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt
    41761 gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta
    41821 aaattgatta gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga
    41881 gccgtgatcg gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta
    41941 acgaatatca ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg
    42001 ggaccaaaag ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta
    42061 gcagttgtct catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa
    42121 aggagttgca gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt
    42181 tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa
    42241 gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa
    42301 atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta
    42361 acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc attttttatt
    42421 actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa
    42481 aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca
    42541 ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc
    42601 gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa
    42661 gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc
    42721 ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat
    42781 atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt
    42841 tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca
    42901 ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt
    42961 ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca
    43021 gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat
    43081 tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt
    43141 ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga
    43201 aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag
    43261 gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg
    43321 atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt
    43381 gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga
    43441 taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt
    43501 cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca
    43561 aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt
    43621 taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta
    43681 aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag
    43741 gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag
    43801 atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg
    43861 aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc
    43921 atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag
    43981 acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg
    44041 caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac
    44101 gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt
    44161 tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt
    44221 gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc
    44281 ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta
    44341 aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac
    44401 ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc
    44461 tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa
    44521 aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa
    44581 atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga
    44641 ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa
    44701 cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct
    44761 gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata
    44821 ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc
    44881 cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa
    44941 ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc
    45001 gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg
    45061 gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat
    45121 ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt
    45181 gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat
    45241 gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg
    45301 atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc
    45361 gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc
    45421 actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta
    45481 tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc
    45541 ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta
    45601 cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa
    45661 gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta
    45721 tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct
    45781 aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg
    45841 gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg
    45901 ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga
    45961 gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag
    46021 gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg
    46081 gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt
    46141 cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg
    46201 atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc
    46261 aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg
    46321 tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag
    46381 caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg
    46441 gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta
    46501 gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa
    46561 tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta
    46621 aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat
    46681 tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat
    46741 ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga
    46801 acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag
    46861 caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga
    46921 atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact
    46981 tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata
    47041 caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa
    47101 tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg
    47161 tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt
    47221 tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg
    47281 gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca
    47341 gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg
    47401 cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc
    47461 tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg
    47521 ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac
    47581 gattagccga acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg
    47641 gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt
    47701 acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg
    47761 ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc
    47821 gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt
    47881 agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt
    47941 gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat
    48001 tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg
    48061 attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt
    48121 agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat
    48181 gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag
    48241 agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg
    48301 aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc
    48361 attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga
    48421 agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa
    48481 agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag
    48541 ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt
    48601 acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca
    48661 cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt
    48721 cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg
    48781 aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag
    48841 atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc
    48901 aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg
    48961 gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa
    49021 aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa
    49081 cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat
    49141 tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg
    49201 cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct
    49261 acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga
    49321 aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt
    49381 aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg
    49441 tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga
    49501 attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg
    49561 aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg
    49621 cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc
    49681 acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta
    49741 tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa
    49801 tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc
    49861 tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa
    49921 ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca
    49981 aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga
    50041 tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc
    50101 aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga
    50161 agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga
    50221 gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga
    50281 aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca
    50341 agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct
    50401 gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga
    50461 agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa
    50521 tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat
    50581 gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga
    50641 tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca
    50701 aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc
    50761 cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc
    50821 agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat
    50881 tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa
//
LOCUS       DASHWH010000013        50032 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.013, whole genome shotgun sequence.
ACCESSION   DASHWH010000013 DASHWH010000000
VERSION     DASHWH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.013"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(35..1045)
                     /locus_tag="U2R41_000772"
     CDS             complement(35..1045)
                     /locus_tag="U2R41_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8888203.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1225..1569)
                     /locus_tag="U2R41_000773"
     CDS             complement(1225..1569)
                     /locus_tag="U2R41_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8888204.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1562..1996)
                     /locus_tag="U2R41_000774"
     CDS             complement(1562..1996)
                     /locus_tag="U2R41_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEM8888205.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2134..2871
                     /locus_tag="U2R41_000775"
     CDS             2134..2871
                     /locus_tag="U2R41_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888206.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2864..4075
                     /locus_tag="U2R41_000776"
     CDS             2864..4075
                     /locus_tag="U2R41_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888207.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4279..5061
                     /locus_tag="U2R41_000777"
     CDS             4279..5061
                     /locus_tag="U2R41_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEM8888208.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5131..5793
                     /gene="trmB"
                     /locus_tag="U2R41_000778"
     CDS             5131..5793
                     /gene="trmB"
                     /locus_tag="U2R41_000778"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEM8888209.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5891..7645)
                     /gene="ade"
                     /locus_tag="U2R41_000779"
     CDS             complement(5891..7645)
                     /gene="ade"
                     /locus_tag="U2R41_000779"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEM8888210.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7777..8706
                     /gene="tadA"
                     /locus_tag="U2R41_000780"
     CDS             7777..8706
                     /gene="tadA"
                     /locus_tag="U2R41_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEM8888211.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8663..9712
                     /locus_tag="U2R41_000781"
     CDS             8663..9712
                     /locus_tag="U2R41_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEM8888212.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9709..9987
                     /locus_tag="U2R41_000782"
     CDS             9709..9987
                     /locus_tag="U2R41_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEM8888213.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9962..10432
                     /locus_tag="U2R41_000783"
     CDS             9962..10432
                     /locus_tag="U2R41_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEM8888214.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10449..10751
                     /locus_tag="U2R41_000784"
     CDS             10449..10751
                     /locus_tag="U2R41_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888215.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10768..11139
                     /locus_tag="U2R41_000785"
     CDS             10768..11139
                     /locus_tag="U2R41_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEM8888216.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11111..11470
                     /locus_tag="U2R41_000786"
     CDS             11111..11470
                     /locus_tag="U2R41_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888217.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11642..12649
                     /locus_tag="U2R41_000787"
     CDS             11642..12649
                     /locus_tag="U2R41_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8888218.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12674..13858
                     /locus_tag="U2R41_000788"
     CDS             12674..13858
                     /locus_tag="U2R41_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEM8888219.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14295..15878)
                     /locus_tag="U2R41_000789"
     CDS             complement(14295..15878)
                     /locus_tag="U2R41_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8888220.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16456..16599)
                     /locus_tag="U2R41_000790"
     CDS             complement(16456..16599)
                     /locus_tag="U2R41_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEM8888221.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16822..17301)
                     /locus_tag="U2R41_000791"
     CDS             complement(16822..17301)
                     /locus_tag="U2R41_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8888222.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17374..17658)
                     /locus_tag="U2R41_000792"
     CDS             complement(17374..17658)
                     /locus_tag="U2R41_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888223.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17736..17930)
                     /gene="ssrS"
                     /locus_tag="U2R41_000793"
     ncRNA           complement(17736..17930)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U2R41_000793"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18002..19288)
                     /locus_tag="U2R41_000794"
     CDS             complement(18002..19288)
                     /locus_tag="U2R41_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEM8888224.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19426..20097
                     /locus_tag="U2R41_000795"
     CDS             19426..20097
                     /locus_tag="U2R41_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEM8888225.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20063..20551
                     /locus_tag="U2R41_000796"
     CDS             20063..20551
                     /locus_tag="U2R41_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEM8888226.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20564..21511
                     /gene="prmA"
                     /locus_tag="U2R41_000797"
     CDS             20564..21511
                     /gene="prmA"
                     /locus_tag="U2R41_000797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEM8888227.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21512..22276
                     /locus_tag="U2R41_000798"
     CDS             21512..22276
                     /locus_tag="U2R41_000798"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEM8888228.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22509..24722
                     /locus_tag="U2R41_000799"
     CDS             22509..24722
                     /locus_tag="U2R41_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEM8888229.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKEKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24733..25179
                     /locus_tag="U2R41_000800"
     CDS             24733..25179
                     /locus_tag="U2R41_000800"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEM8888230.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25350..26609
                     /locus_tag="U2R41_000801"
     CDS             25350..26609
                     /locus_tag="U2R41_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEM8888231.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26698..27645)
                     /locus_tag="U2R41_000802"
     CDS             complement(26698..27645)
                     /locus_tag="U2R41_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8888232.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28039..29331
                     /locus_tag="U2R41_000803"
     CDS             28039..29331
                     /locus_tag="U2R41_000803"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEM8888233.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29346..31115
                     /gene="aspS"
                     /locus_tag="U2R41_000804"
     CDS             29346..31115
                     /gene="aspS"
                     /locus_tag="U2R41_000804"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEM8888234.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31224..32099
                     /locus_tag="U2R41_000805"
     CDS             31224..32099
                     /locus_tag="U2R41_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8888235.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32365..34248
                     /locus_tag="U2R41_000806"
     CDS             32365..34248
                     /locus_tag="U2R41_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM8888236.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34323..34607)
                     /locus_tag="U2R41_000807"
     CDS             complement(34323..34607)
                     /locus_tag="U2R41_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM8888237.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34597..35331)
                     /locus_tag="U2R41_000808"
     CDS             complement(34597..35331)
                     /locus_tag="U2R41_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEM8888238.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35460..36083
                     /locus_tag="U2R41_000809"
     CDS             35460..36083
                     /locus_tag="U2R41_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEM8888239.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36275..37396
                     /locus_tag="U2R41_000810"
     CDS             36275..37396
                     /locus_tag="U2R41_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEM8888240.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37403..40531
                     /locus_tag="U2R41_000811"
     CDS             37403..40531
                     /locus_tag="U2R41_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEM8888241.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40550..43744
                     /locus_tag="U2R41_000812"
     CDS             40550..43744
                     /locus_tag="U2R41_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEM8888242.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43890..44597)
                     /locus_tag="U2R41_000813"
     CDS             complement(43890..44597)
                     /locus_tag="U2R41_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM8888243.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45362..45760
                     /gene="spxA"
                     /locus_tag="U2R41_000814"
     CDS             45362..45760
                     /gene="spxA"
                     /locus_tag="U2R41_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM8888244.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46092..46745
                     /locus_tag="U2R41_000815"
     CDS             46092..46745
                     /locus_tag="U2R41_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEM8888245.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46971..47969
                     /locus_tag="U2R41_000816"
     CDS             46971..47969
                     /locus_tag="U2R41_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEM8888246.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48145..49953
                     /gene="pepF"
                     /locus_tag="U2R41_000817"
     CDS             48145..49953
                     /gene="pepF"
                     /locus_tag="U2R41_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEM8888247.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16181 a         8748 c        10461 g        14642 t
ORIGIN      
        1 gcgagacata agtcttcgct ttttttgttt tcttttattt tgatgaatca gttgtttctt
       61 cagttgaaga ttcttttgtt gaatcagttg attttgtgtc agaactcttt gttgaagctg
      121 tagtttcact tgagtctttc gtacttgatg aagttgttgt gaatgcactc aacacatttt
      181 caaaggcatc atctttgatt tttacatttg catcttttaa ttcttcaccg attactttag
      241 ttgtgaatgc attgtcactt agtttagttt cagtagcgat atcttttagt tgatctttgt
      301 atttatccat gtcattgcct ttattttggt ttttcaccat tttgacaacg tagtattctg
      361 tcgcataaga agtaggattt gttgtagtaa taacatcgga gatctcaccg tctttcaatt
      421 tgaaagctgc ttcttttact tctgcaggaa tagttgttgt cgttgagtcg aatttaactt
      481 taccgccatc ttcttttgtc tctgtatcag ttgatttgtc tttggctaat ttagagaaat
      541 catctccatc atccgctgat tttttcacat cttttgcttc atcttcactt gataatttga
      601 tgatttgtgc ttctacttct ggatggaaag atttccaagc agttttcaaa tcgtcatctg
      661 taatatctac gtgagctttc aagccagctt caaaagctaa attgttacga atatattctt
      721 tataagtatc tttcgtatat ccagcagctt ctaattgtga ttcaaaagta tcacctaatg
      781 atttcgcttg tttgtcgtat tctgcatcca cttgcttatc ggttacttta tcgccatatt
      841 tgttattgaa tactttataa atgatcatcc gttgtactaa ggattggttc gatgattcaa
      901 gttttgcttc atcatagaaa tcagatacag tgatcgttcc gcctttcatc gttgcgatat
      961 ctttattagt atcgcctgaa caagctgcta gagttaaaac agccaacgca ctagtagctg
     1021 ccaaaataat tgattttttt ttcattaaaa tttgcactcc tgtaattagt tattattttt
     1081 atatattaac tataccatat cccatttcct ttaaaaacgt tgtcgtggta gtttcaaaca
     1141 aaattcaaaa tttcatcaca ttcctaggaa atacgcaaac ttttctcact tatatagtta
     1201 aatctgttga gaaaataatc attattatat ttgttcagga tactctggca gatcagcttt
     1261 tagtttttct acttgttcat tgatttgtgt gatacgtggt tcggcttgga atttaaaatc
     1321 ttcgatatct tttttgattg cttcttggaa agcaggaatc acagattcag ctgtttcttt
     1381 tgtttcgatc aatgcgcttt taaaatgatt caggctgtta tttaaatcat ttgtcagagt
     1441 tgttgcttct tccaattctt cgatcagttt ctttcttgtt tcattcccgc tcctgggcgc
     1501 caataacaga cctccgacag tgccagcagc agctccaaat aacagtcctt tgaaaaagtt
     1561 tttaatcatc gcaggacacc tgctttacga ttgtttcttg aatagctttt agttcttccg
     1621 atccataact atcttgatga ttgacaaagt gaatagaaaa atcatcctct ttgctgtagc
     1681 gagggactaa atggatatgt gaatgaaaaa cagattgata ggctagttca cggttgttat
     1741 tcaagatatt aagacctttc atttctggaa aagctttttc caatgcttga gcaatcttag
     1801 gcacacgcga aaaaacctct cctgcaagtt cgggatcata ttcaaaaata tctgctacat
     1861 gacgtttagg cacaactaat gtgtgtcctt tcgttgcttg agagatgtct aaaaaagcat
     1921 agaccttgtc gtcttcatat attttatagc ttgggatttc ttgattgatg attttgcaaa
     1981 aaatacagtt ttccatataa aacatccttt ctataaagaa taaatacttc agcttaactt
     2041 taccataagt taaagctatt gtaaggaaaa aaacaaaata ccgtcatttt cttcgtggta
     2101 taataaataa aaataaatgg cggaggaaaa agaatgagtt taattatcga tcatgtcaca
     2161 ggggggtacg gacatctccc tgtgttaaaa gatatcagtt tcgatgtaca gcctggagag
     2221 atagtagggt tgattggact gaacggagca gggaaaagta cgaccatcaa gcacattatc
     2281 ggtctgctgg aagcttcaaa aggaacgata gaaatcaatc atctatcctt aaagaaaaat
     2341 acggaagaat atcgcaaaca aattgggtat attccagaga ctccttcttt atatgaagaa
     2401 ttgacattga gagaacatat cgaagtcact gcgatggcct acgatatacc tatggatata
     2461 gcaatgaaaa gagcagaaaa gttgttggag acttttcggt tatctaatcg tcttgactgg
     2521 ttcccagcta atttttcaaa gggaatgaaa cagaaagtga tgatcttatg cgcctttttg
     2581 atcgagcctt ctttatatat catcgacgaa ccatttttag gcttggatcc actagctatc
     2641 aatgcattgc ttgaattgat ggtagaaatg aaaaaagaag gagccggtat tttgatgtct
     2701 acgcatattt tagctactgc agaaaaatat tgtgatcgtt ttgtggtctt acataacgga
     2761 gagatacgag cagacggttc tcttgagatg ttgagaaaag aatttcagct gccggattct
     2821 tctttggacg aaatctatat tgctttaaca aaggagcagg cacatgagta gtttttttca
     2881 aaaacgttta gcaagacatc aaaagaaaat gatgaaatat atgcgttatg tattgaacga
     2941 tcattttgtt ttagtttgtc ttttcttgat tggcggtgtg ggattatatt attctaattg
     3001 gttgaaaacc ctgcagccac cgtttgcaat tggagggctc ataatctctg tattttggat
     3061 gctctgttta ttcgtgggta agatggctac ttttgcagag gcagcagata ttgttttttt
     3121 acttcctaaa gaaaaagaaa tgcaaaccta tttggaacga gcttttcgct attcttgcat
     3181 ttttccgttg atctttttga tattggcatg cggattggcc atgccattag tggtggttag
     3241 tacggagcaa tccttttcga tgtttttcgt ctatctgatc atattatgga gtttgaaatt
     3301 cagtcatctg caaatcaaga gagcagaatt gtttcgacta gatcaacaag ttattcgtta
     3361 ttggaaatta ggctggtttt tttcttctct gatctttttg tttctatcat tttattggct
     3421 aatatgggca ggaatagcag gtgcattagt acaggcaagt ctatattact ggtttttgtg
     3481 gaagcaacaa gaaaaaggat tggattggga gaaaatggtg catgtagaag aacataggtt
     3541 gcatcagatc tatcaattta tcaatttatt cacagatgtt cctgaaatta cggcaaagat
     3601 caaaagaaga cgctatttgg atcctatatt gactcatatt aaaagagaat caaaaaacac
     3661 ttatctttac ttgtttgcaa gaagattggc aagaggttct gattttagtg gtctttattt
     3721 ccgattagta attataggtg ggttattgat tgccggagta gtcgactttt attttagtct
     3781 gattatcggt ttcttatttt tatatttgat cggtttccaa ctgttacctt tagcgaacca
     3841 gttccgctat atggttctcg ttcagcttta tccaatccat aagaatcaga agaagcaggc
     3901 gattcagtat ctactcacat gggtattaat tatcgcgtcg gttatttttg gtttgattgc
     3961 tgcagtcgta ttagatgggc agcagcgatg ggttccgcta gcggcttatt tgattgttac
     4021 tatgctcttt gttaaactat atgtaccaac tcgtttgaag aagatgtcag actgaaaaca
     4081 aagctgtgta gggtaaaaat gagaatgaaa aatagagcgt tgacagtaag aatgctgttc
     4141 gttctatttt ttttgagaaa agctggtttt ttgaatggca attaaatgaa aaagaacgta
     4201 tatgtgcttg acgtgtagtg ttcaggcgac tagaataata agtactgcaa aacaaagagt
     4261 aagaggacgg gagaatgcat gatggagttt cagttagacc aagagtggcg gttacgtccc
     4321 atcaaaggcg acacaggaaa aacatacata ggcatgcgtg atgaggacaa agtctttatt
     4381 aaacgaaaca cgactccgat gttggctgct ctttcaaggg aaggaattac accaaaactt
     4441 gtttggacaa aacgaacggg gaatggcgat actttgacag cacaagagtg gctggaaggt
     4501 cgcttgctga ctcctaatga gattggtcaa agaaacgacg tgattgatgt tttgtaccat
     4561 ttacatcatt caaattctct taaaaccatg ctgacccgta tcggtggtcg tgtcatgacg
     4621 ccggagaaga tgctgcaaaa atatgaaaaa gaattaccgg aagaactcca gaaaaatcaa
     4681 tttctgcaaa ttgtccatcg ttttcttcgt gagcagatcc cgtcaataga taaggaaaac
     4741 ttatcagtag tacacggaga tgtaaaccat cgtaattggt tagtatgtaa aaattattta
     4801 tatctagtcg attgggattc aatcatgttt gcagaccctg cggtggatat tgcaacgatt
     4861 ttaggaaatt atgtgccact gtctagctgg aatcagtggc tgatcagtta tggcatacgc
     4921 ccgactgacg aaatgcttga aaaatttcat tggtatgcgg ccatgaattt gctccaagaa
     4981 atcaccagat attgcaaacg tgaagatcat cgacggatga acgaagaaat tttacaatta
     5041 aaacgaattt tcagtggctg aggtttggcc caaactctgc ggagatttgg ccagaccttt
     5101 ttaattagag gaaaggaatg gagataaata atgcgtgtcc gaaatcgtcc gggagcagcg
     5161 gaaatgttag cggctcaccc gaattttgta attagtgatc caacattgtg gaagggaaaa
     5221 tggaatgaat tgtttgaaaa cgatcatcca atccacattg agattggtat ggggaaaggc
     5281 cagttcatca ctggaatggc aaaagcccat cctgaaatca attatattgg agtagaaatg
     5341 caggtcagtg ttgtttcgat tgcactagac aagttgatcg aacaaccgct tcctaacctg
     5401 aaacttctac atgtggatgg atcagcgttg acagagtatt tcgctgacag cgaagtagat
     5461 caaatctatc tgaatttttc cgatccatgg ccaaaaaaac gtcatgaaaa acggcgattg
     5521 acatataaga cttttcttgc tgtagatgaa cagattcttc gtccaaatgg agaaatccat
     5581 ttcaaaacag ataatcaagg attgtttgag tattctctgg caagtttttc tcaatatggc
     5641 atgatcctca aacaggtatg gttagattta catcaaagtc aatttgaagg caatattatg
     5701 acggagtatg aagaaaaatt ttcttcaaaa ggtcaacgta tttatcgagt agaagctcaa
     5761 tttcaagata agaccaaaaa gaaaagtctg tgaaactaac tttctgcttt cataaaaaca
     5821 ttaatatcca taaataaatc ccagaaaatg aacattacag atgtaatgta ttttctggga
     5881 tttatttgat ttaaaatgta tttatttcat cggtttttcc acaaggtgga atcaattgga
     5941 agcgagaaaa tgaaaataat cctttgtcgg tgatcttcaa ttcgggtatg acagataatg
     6001 ttaagaaaga aagtgtcaaa aaaggatcaa aggttttagc tgctcctagt tgatgtgctt
     6061 tagttattaa ttcattcaac tgttcataga cgatcgaaaa aggttcctgt gtcatgattc
     6121 cgcctatagg aagagaaaca caagctattt cctgctgttt gttgacaacg gtcatcccgc
     6181 cacctttttg aatcaaaaga ttagcagcat acagcatatc ctcgtcgttt gtcccaacga
     6241 caactagatt atgcgagtca tgggcaatcg ttgtcgctat cgctccttgt ttcagctgaa
     6301 atcctttcac tatgcctaat ccaatgtttc ccgtttgatg gtgacgctcg atcaccgcaa
     6361 tctttaattg gtctttttcg attgaaggaa tgaatcgttc gccttgtcgg tccacttttt
     6421 ctaccaaatc tttagtcact agactattcg ggataatttc gatcacatgg gcttgtgaag
     6481 aagaaagaag cagctctaac gaatgtggat ttaatggttg agcgtttagt tttggcagac
     6541 aacacgtata tgaatttgaa tgatcagcaa ataaatggtc ttggagttgc ccagattgaa
     6601 caactaatgt tcccttatag aaaacttcat gaattggcag cttggtaaga tcgtctgtta
     6661 agaagaaatc agcttgatac ccaggggcaa tcgcacctag tgttttcaaa tcaaaacatt
     6721 ctgctgcatt caatgttgcc atctgaatgg cttgtaatgg atcgattcca agtgcaatag
     6781 cttgacggac gatatgatcg attgatcctt cttttactaa atcattgatc agtttatcat
     6841 cggtgacaaa gaagcatctt cttgaatttt caggtgtgat cgccggtagt agcgatgcca
     6901 gatcttttgc aacggtccct tctcgaacca ttaaatacat accaagatcc aaacgttctt
     6961 tagcttcttt tgcagttgta gcttcatgat ccgtccgaat acctgctgca ggataaacat
     7021 ttaactcttc catgccgata ccagctgcat gcccgtcaat ttttttctta tgctggtgca
     7081 tcaattggat cttttctagg atgtctgttt cattatttgc tactgcttga tagttcatga
     7141 cttcagctaa gcctaaaact tcgggttttt gtaaaaaagg ggctaatttt tttgcattta
     7201 aatttgcacc attggtctca aaaggggtta caggtacaca agaaggtagc ataaaaaaga
     7261 tattcatgag tgtttgtctt gcatcttcta tcataaactc aagacctgta ctcccagcaa
     7321 tgtttcctat ttcatgggga tctgtcacta cagtagtaac tccatgaagc aaggatactt
     7381 ttgaaaattc gcttggtgat aaaagtgagc tttcgatatg cacatggcca tcgatgaatc
     7441 ctggcactag atattgccca ttagcattat gtacgctttt tccttcgtat tcaccgattc
     7501 ctacgatcat tccttcgcag attgctacat ctttttccat gatttctttc gtaaagacgt
     7561 tgacgatctt agcatgcttg atcactaggt cagcaggctt ttttttactt gctacttcga
     7621 tatatttcct taatgttgtt tccaatggag tgcctcccaa gataaatttt tataagtata
     7681 acataaattc ggtttttgcc aatatttttt tgtaaattca ttttatttga aacaaaaaga
     7741 tcaaaaagac gtatgttttt tgtggagggg agaataatgg aaataaaacg attatcggat
     7801 caaatgataa gtgaaagttt tgccaaacat gcccaagacg tatatatatt gccagcttca
     7861 gaagtatttt acatctattt tcgctatgga aaaaaacgaa cattctggaa gaaagtctct
     7921 tcggcagatg gacagcaact gatctcaaga tttaagtatt tgggacagat ggatatcgga
     7981 gaaaaaagaa aagctcagtt aggtgcattt acttattctt tagaaaaaca ggaagttcgt
     8041 ctgcggttgt cttctgtagg agattacctt caacgagaga gtttagtgat tcggctatta
     8101 tacggtactt ttgaaacacc ccatcgttgc tttagttctc gagattttgc aactatttta
     8161 cagaaaaccg tcagacgtgg attatacctt ttcagcggac cagtcggatc aggcaagact
     8221 tctctaatgt atcggttggc tttggaagaa aaattgcaag tgattactat cgaagatcct
     8281 gtagagatag aagaaccgtt ttttctgcag ctacagatca atccgaaaat agcacagact
     8341 tatgatacac ttttgagact cgcattacgg catcgtccag acttgctaat cattggcgag
     8401 ataagagatg aatcaactgc acatgctgcg gtaagagcag cactaacagg gcaccgggtt
     8461 tttgcaaccg tacatgcacg caatcttgaa ggtacattgg tcagaatcca tgaactgaca
     8521 aagaaaaaag aagagctatc tgaatgttta gctggtgtca tttatcaaga acttttggaa
     8581 gatgccaatc accatccttc tgttttatgg agttatcaat ttatggagga agaatcagta
     8641 aaaaaatcat gggaggaaag ttatgaagaa gctcaggaaa atatttggca gtcagaacta
     8701 ttctaaagga ttaacaagaa gccagcaaaa ccagtttgca caaaccatgg gagaactgct
     8761 tttatctggt tttagtctgc aagaatccct ttatttatta ctcacgatcc gttcgtttcc
     8821 acctgttatt ttggcaaata tccaacatca gctaaaggaa gggcaagcgt ttagtgaggt
     8881 actttcgcaa ataggatatg gagcagagca actggtacag atcgagttag ctgaacgtca
     8941 tgggaatctg ccgacaactt tgttggaaat tgccaaacag tttcggcttg ttgaaggatt
     9001 tcgcaatgat ttgaaaaagc tgcttgcata tccgatgcta ttgctcacct ttttgctagg
     9061 tatcttgttt agtatgcggt tgttcgtttt gcctcagctt attggttcag gaatggtcat
     9121 gcaagagcat tggggtattc agctgataca agtctttcat tggtatgttc tactattcat
     9181 cctagcggtt actctattgg ctttactagt aaggtatcgt ctgatgaagt tgtctcctgt
     9241 tgatcagagt gaatggataa gcagacaatt attgattgga ccgatatatt ccttgtatca
     9301 atcggcttat ttgtcactag agattggaaa gctttttcat gaaggtttcg aattaaggca
     9361 aatcatagtc tgcttaaagg aaacgaaaca agaaagtctg attcagcatc ttgcctctaa
     9421 gttgattcaa ggattggagg caggtatgtc attggctgag cagttccaga attatcgttt
     9481 tctatcagaa gaattcagta aaattgtttt acagggagag gcaaagggaa gtttaggtaa
     9541 agaactgatt ttttacagcg aatggacaag aaaacagctt ttcaaacgtc ttcatcactg
     9601 gatgcatttt attcagccaa tcattttttt gggcgtagcg gctttgattt tattagttta
     9661 tgccgctgtt ttattacctg tttacggaag catagaggag gttttgccat gaaaaagaga
     9721 aaaatatctt attatagcgg ttttacattg atcgaaatgc taatcgtctt actgatcatc
     9781 agtgtattag tgttgttgtt cgtccctaat ttgtcgcgtt ataggaatca tgtagaccaa
     9841 gaaagtcgtg aagcgatcat ccagttggta gacacacaaa aagagctcta tgcgcttcaa
     9901 aataatggaa gagttcctac tgtggaggaa ttgttgaatg aaggctatat caaacgagaa
     9961 catgcagaaa tctatcagcg gccttgaagg taaggtttta gcatcaggat atacgttgtt
    10021 tgaatcactc ttactgattt tattacttac aacgatcctt tcttttccag ttctcgcatt
    10081 ttctgcatgg aagaatgaat tggcagtcta tcaatttttc ggccaatttg aaaaacgaat
    10141 ctattctacg caaaaaatcg ctattgtgaa tcagatccaa acaggctttt attggaatag
    10201 tgaagagaat caaatcattt ttcatgttcc gaatccagat aaaataaatt gggaagtatt
    10261 agatattcct gatgagatta ccttgaaacg ccatgcttct attacttttg ctgctagaac
    10321 cgggaatgaa agttcactaa aagcttatca attttattgg gaagaaaaag agcggacgat
    10381 tacgtatcaa ttccagatag ggagtggtcg ctatacaaaa agaattgaat aaaacaggat
    10441 ttgttttgtt ggagtccctt acagcgctta tgatcgcttc tgtctgtgtc tacttagtga
    10501 gctttggcca gctgtttttg cttcaggcag aaaagaaaag tggggaggaa ctgctattta
    10561 ttcgaatgat ttatgaagat atcaagacgc agcggatgta tcacaaaaga ccagagcgaa
    10621 gggaagaaat gatcccatac gatatccaaa ttgtcgataa taatggagta atcgaaaaag
    10681 tgtttgtcaa tatggagaaa gaagcgatca gtattgaaaa aacaagtaat tccagcattt
    10741 acgctgattg aatgtctaat tggactgatg atattgagca gtctattgct cacattcaat
    10801 cttttgattc agcaaaccat ccaagtaagt gaatttttgc agcgtaaaga tcaaaaagag
    10861 tggctaatat ttttagcaca attagaagaa gaactcaaga gcagtcacaa tgtctctgtg
    10921 aaaaatcagc aactgcacta cacgatcgag gataaacagt atatcatcga aagatatcag
    10981 gcaatgatac gtaaaagaag aacttccggc ggacatcaac ctatgttaac aagtatcact
    11041 gagcttcaat tattggaaaa agaacacaca atttcttttt atgtacactt tgaaaatgga
    11101 gaagatggat atgccatatg gacaaaaaac gatcagtaga aaatgttata agggctctat
    11161 tttattttct gtcttgcttt tatttctttt atgtacgttt ttattgatgg cagcattaga
    11221 aagctaccgt ttgtcaatgg atcttaatat acgaatcaaa aattattata tagcaaaaat
    11281 aatgaagcag atggctttat cggaacagtc tacgcttgcc gaaagttcgg aaggagtttt
    11341 ttactacaca actggatctg taacatatca gtgggtacaa cagtccctat tcttagaggt
    11401 ggaagtctca ccgtttatct ttcggttcac agaagaagta aaaaatgata cagatactag
    11461 tacagaatga tggaatgttc gtgaaaacgc cctcttttct gttataacac tgtttttaat
    11521 aaaaaaatgt ttgccgtttc acaaataaaa aacagtgaaa acgattgaaa ttttgtcaat
    11581 tgttcgatag aattaaacag gtgaaaaaat tgttataata tatctagaaa tgaggtgcac
    11641 catgttacca gaaaaaatgg aacaagggtt taatcaaaac ctggaagcta ttcagctttt
    11701 acagaatgca ttgggaactt cctttttaga agcctatgtc gaaaatgcag aaaatttaat
    11761 agatgattat caagtacgtg tggtggatgg agtgccgaca aaagagacaa cgcaacggat
    11821 cacagctctt tatgaagagt tgaagaaact ttctttcgag ccagaagaat ggcgcagact
    11881 gtctcaatta cttttattga agggcagcca gacagaacat ttgcaggcga accatcaact
    11941 cactcctgat agtatcggtt ttttatttgt ttttcttatt gaacaactat acacaaataa
    12001 aaaagcaccg gttaagattc tggatattgc ggcaggaatg ggcaacttgt tattaacagt
    12061 tcttttaaac ctatcgaatg cggggtacca aacagaagga attggtgtgg atattgatga
    12121 tacattgttg gctgtcgcag cttctacaag tgatttgaca caagcaaatg ttcaatactt
    12181 tcatcaagac gggttgcagg aattattaat agatcctgtc gattttgcaa tcagcgattt
    12241 gccaatcggt tattatccga atgacgaaaa agcaaaagag tttttaacaa gtaccgaaga
    12301 aggccatagt tatgcacatc atttattgct agagcaatca atgaaatatg tgaagccgga
    12361 tggttttggt ttgtttttga tgccgagtgg atttttagaa acggatcaaa gtgaagaaat
    12421 caaaaaatgg tttaaagaag aaggttatct tcaaggaatg attcaattgc cagatgaatt
    12481 gttccgtaat aaacaatcgc aaaaaagtat tttgatctta cagaaaaaag gaccacaagc
    12541 acaacaagta aaagaagtct tgcttgtgaa acttgcttca ttaaaagaac cagagaaggt
    12601 gacggaattt ttcaacgagt ttaaaaactg gaaatcatct gctctgtaat cattcaacga
    12661 gaaggagagt catatgtcta aaacaattgc aatcaatgct gggagttcaa gtttgaaatg
    12721 gcaattatat caaatgccag aagaaaccgt agttgcgaaa gggatcgtcg aacgtatcgg
    12781 tttacaagat tctattttta ccatcaagta cggcgataat caaaaattcg aacaaatcat
    12841 ggatatcgaa aaccacgaag tagcagtaaa aatgctgtta gatcagttga ttgaactgca
    12901 aattttaagc tcttatgatg aaatcaccgg agtagggcac cgtgttgttc acggaggaga
    12961 aacttatggg gattcagtag tgatcgatga gaaagtaatg gaccgcattg ctgaattagc
    13021 agaatttgct cctttgcata atcctgcaaa cttaatgggg atcaaagcgt ttaaaaagat
    13081 tttacctgac attttgagtg tggctgtatt cgatacatct ttccactcaa caatgccaaa
    13141 gcaaaattat ttatatagct taccggtgtc ttattatgaa gatttcaaag taagaaaata
    13201 tggcttccat ggcacaagtc atcgttatgt gtcggaacgt gcggcaaaat tattgaacaa
    13261 accgatcgaa gaactaaaaa tcatcacttg ccacttagga aatggtgtat cgatcacagc
    13321 agtcgatgga ggtaaatcca tggatacttc tatgggattc acaccattag ctggggtgac
    13381 aatgggtaca cgttcaggtg atatcgatcc agctgttctc ccatatttaa tggaaaaatt
    13441 agagattgat attgatgaaa tgatcaatgt tttgaacaag aaatctggtc tgctaggcct
    13501 aacaggattg tctagtgata tgcgtgatct tgaaaagaac tatgaaaaag aacatattcg
    13561 tttagcttat gacattttcg tggaccgcat ccgaaaatac atcggcggtt atgtgacaac
    13621 gatgaatggt gttgatgcaa ttgtctttac tgctggtatc ggcgagaatg acggacatgt
    13681 acgtagtgaa atcatcaaag gtatgtcttg gtttggttgt gaaatcgatc cagcgttgaa
    13741 tgaattgcgt ggtgaagaag tagatatctc tacaaaagaa tcaaaagtaa gagtattagt
    13801 gatcccaaca gatgaggaac taatgattgc ccgtgatgtg gagcgtctgc gcggctaagc
    13861 caaagcattc gttaggataa aagatgaaac gcatgcaaga gggaattctt gcatgtgttt
    13921 tttttgcgga gaagaccgca aaatggtaat ttaacttcaa tttcccttct tttttacagg
    13981 atcttcttag ggaattttgc ttggaaaagt ggtcaaaagg gaaaagactg gcattttatt
    14041 tattgatttt caggaaaatc ccaatatttt attttcattc atctttataa gggacattac
    14101 atatgtaaaa agaaaaaaca tttttagatc agcctcgtgg gctgtcgact ttattcttcg
    14161 ctttatgttt agctagaaaa ccgatccgaa acattcattg atcgaataat aaaaacttga
    14221 ttttccacct gtactttgcc tgaatcgtga aagcacaggt atgaaaatca agttttttgt
    14281 tgcgaaaaaa cgtcctagtc aatcagttgg ccgagcggct ggcggctgat ctgctcctga
    14341 tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt agcggtcatg gctgactgct
    14401 aggagcgttc cttgatagtc ttgcagcatt tcttcaattt cttctcgagc atagctgtcg
    14461 aggtgattgg ttggttcatc caaaatcagg acgttgattt tttctaaaaa gagtttcagc
    14521 aacgccaaac ggattttttc accaccggat aaatctttga tccgtttacc cacatcgctt
    14581 tgataaaagc cacttttggc taattgccgt cgagcgactt cttcgtttcc agtcaatgtg
    14641 atggcgtaag ccagcaaacg gctatcggga tcggcaaact gcagctgctg cggcaaataa
    14701 ccgatttgca agctactacc taatttgatg gtgccaccgt aaggcttttc ctcaccgagt
    14761 aataatttga gtaaggttga ttttcctgca ccattgtcgc cgatgatggc taaatgatct
    14821 ccacgataga ctgcaaaact gctgtctttg aatagtactt gatcagcgta ctgctggtgc
    14881 aagttttgaa tgatgaaaac ttctttgcca gattttccac cattagacaa gctttgcagc
    14941 cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt tcgccaggcg gcgttccagt
    15001 tctttggctt ttttgaagaa ggactcattg tctccttcat gtgcccactg acgaaaacga
    15061 cgaatcatga ctttcaaccg ctggacttct ttttgctgca gttcgtaatt ttttgtcagc
    15121 tcagccaagc gagcttcttt caagactcga aaacggctgt agtttccggg atactcaatc
    15181 aatgcacctt cttccagttc ccatgttttt gtggtgactt gatcgagaaa ttgccgatca
    15241 tgagaaacga ccagaaccga tttttttgta ttctgtaaat agttttccaa ccagcgaata
    15301 ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa gtaaatcggc atcggctgct
    15361 aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc taagttctgt taaccgtgct
    15421 tcctgacggt gtggaatccc taagccttgc aagatcgctg caatccggtc ttctaatacg
    15481 taaccgccta gttcttcaaa gcgatgctgc agctcaccgt aacgggccag acacttctct
    15541 aagtttgtat ctggttctgt catcaatgct tcgtagcgac tcatttgctg tttgaggtcc
    15601 tgtatctcag cggcagaatg gagcaagtag tggttgacgg tcgcttcgga aactgtaagt
    15661 tcttgttcga ccgtcccgat ctttaagcct tttttgcgac tgataacacc gctgtcgaca
    15721 ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc cagttccgtt gataccgact
    15781 aaggcaattt tttcaccagc ttcaatttga caggataaat cctcaaataa tggattcccg
    15841 ccgattgttt ttttaataga aattgcttgt atgatcatga tctctctcct tgagaccact
    15901 caaaaaggcc atggttatcc atggcctgtg ttatttaaac tcagattatc atgagaaaaa
    15961 taccttgaga tggccattga ttttacgtga attcaactgt tttggacaaa cttgtcccgt
    16021 caacagcaac tgtaaaacca attgcatggc taaatgcgat atggtatcgt tggtaatttt
    16081 cattacttgt tattttagtc atgcgcatca tagtattttc cctccttcgt gtagttgtct
    16141 tttccagtgt acctgaaagt gtcagaaagt tcaactagcg ctgacattta cttaatattt
    16201 atcgatgtgt aattctgtca gagagctttt tttatctgac tgagggtaat gtctcgtgga
    16261 ccacatcatt gtattctgag ctaaaccagc gttcaggcga aatcatatgg tgtttgataa
    16321 aaaactttgt ctacatgact tccttttgcc atttgcttaa taccgttgca ttaaacagct
    16381 cttctctttg gaaatctcac caacatcgcg ttcaggagta tcgttgtggt tgcaatggca
    16441 agcggtagcc tttcatcaat tcttgaatga tttaccgaat gtagcacttg ccttctcgcg
    16501 gtacccgaac ccgaaataaa attttagttt ggcgataaaa tcggtatccg ccatcgatac
    16561 ttcatatttt ttcgtatcag attcatcgga aacttccaca ttgacgaacc aataagcttt
    16621 ggcatgctta ggtcgtttta aaccacacca gggcgactaa tcgccctggt gttcactttt
    16681 ttttatttta cttgaactca ttgactgttt tccctttatt ttaaacatta taaagaccga
    16741 tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt gtgcaaaaaa acctgccgat
    16801 agttggcagg tcgtctacgt gttaagcttt ggtgtggtct ttagctggca attggttttc
    16861 taaatcagtt cggacaacaa gcacatcgca agctgcgttt ctaataacat attcagaaac
    16921 agaaccaata aataaacgtt ctacagcatt caatcctgtg gctcctaaca taatcaaatc
    16981 tacttgatta tcttctggga tttctctggc aataatttgt tttggtgagc catattctac
    17041 gacagaggtt acattattca agccagcttt cttcgcattg ctttcataat ctgccaatgt
    17101 ttgctttgcc atttcagtag cttgttctgc aagcattcca tcaaaggatg aaacggtttg
    17161 gaaagaacgt gtgtcaatga catgtgctaa tagaagttct ccgttatttc ggatagctac
    17221 attgaccgcc tttttaaaag ctaattcagc ttcatcagaa ccatctacgg caaccatgat
    17281 ttttttgtat tgttgtaaca taagcattca ctcctcaagt caatcgtatt gtactctttt
    17341 attgtagaac actttaggga aaaagagaat tatttactgc tttggttcag caattttttt
    17401 tgcaaatcgg atactgaagg tggcagaaac aataattacg atcagtaaaa agaccaaggc
    17461 aataaagcgt tgattgaaaa aagctgttaa aatacccatt acgcctaaaa cagcatatgc
    17521 agctccgtaa aattgtagga atccttgatt tttatcattt ttgttgatca atacaaaaaa
    17581 gatatcctgt ttattaaata aataatagct aacgaataat aatactaaag tggcgataat
    17641 cattaaaatc ctaatcatga acaaatcctc caaaataagc aacaaataga aaaagcaatt
    17701 tgccaagagc aaattgcttt attctttaaa agatggtaat ctccgttaat gccgttgttt
    17761 gtcctgtagt attgatcccg cgaattcaag caggtgggtc ccatggtcgt agcttcgaac
    17821 taccaaatga taaggcatgt taccagcagt gacacaagac gggtcccaag atatcaataa
    17881 aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc acagatttcc cacctctata
    17941 gtagcatatg aatttaaaaa gagcaatgag agagctcgca gaaacagaac cgttttcttg
    18001 tctagttttt tggaggatgc tttttccact cttttatacg gtaatactgt tgacccagag
    18061 cctgttcata tttccctgta tcttttggct gataatactg agcattcttc agtttatctg
    18121 gtaaatattg ctgattgacc caagcctgat caaaatgatg aggatactga taacccacgc
    18181 cacggttgag tgatttcgca cctttgtaat ggctatctct caaatgatca ggaacatctc
    18241 cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg
    18301 attttggcga caagcaaaga tcgacgacga cgtctgctag tgggatacgt gcctcaggca
    18361 atcccaattt ttcagcagct aatacagcgt tcactgtacg agccgcagca gcaggattac
    18421 ctaaacctat atcttcataa ccgataacca tcagacgccg gcaaatactt gccagatcac
    18481 ctgcctcaac taaacgggcg agataatgaa gtgcagcatc cacatcactt ccacgaatcg
    18541 atttttgaaa agccgaaata acgtcataat gagcatctcc atttttgtca tgggtaagag
    18601 cttttctttg gacacattct tcaatgatcg acagtgtaag atgaatcctt ccttctttat
    18661 ctgaaggagt cgatagtgta gccagttcca gcccattcaa tgcactccgc aaatcgccgt
    18721 tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa ctggattgct tgttgtccaa
    18781 ggccgcgttc tttatccctt aaagcatgtt cgactgccaa ctggatgtct tgttctgtta
    18841 atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc tggattgatg gtaatataag
    18901 gattctcagt agtcgctccg atcaagatga ttcggccact ttctaaatga ggaagcagaa
    18961 aatcctgttt tgttttatct aatcggtgta cttcatccaa taagaggatc actgtaccac
    19021 tcatttttgc ttcttccgct acaacttgta gatccttttt tgaatctgta gcggcgttta
    19081 gcatgcgaaa tgcataatta gtagagccgg cgatcgcact tgcgatactc gtttttccag
    19141 taccaggagg tccatataga atcatggaag agagcatgcg agcatcgacc atgcggcgta
    19201 tgatttttcc ttccccgact agatgctgct gaccaacgac ttcatcgatc gttcgaggtc
    19261 tcatgcgata agccaatggc tgttgcatag gattttcctc cttcttgttt ttacgtccct
    19321 taattatatc atatttagca agatgcctag aaagaacagc cttctgttgt tttgttttca
    19381 tagcttatcc aactaatatt gtgtatgata aagtaggtga gaaaaatgac ggataaacaa
    19441 aagatagatg aatttttttg gaataaaacg acgccagaag tggcacggga tttattgggg
    19501 atgtatttgg agcatgacac accagaagga agattagctg gatatattgt agacgcggaa
    19561 gcatacctag gtccagaaga cgaggccgcc catagctatg gtttaagaag aacaccgcgt
    19621 gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt acacgatgca tactcatcgg
    19681 attttgaaca tcattactca gccagaaggg attcctcaag gagtaatgat acgtgcaatc
    19741 gaacccgccg caatgattga tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat
    19801 atcagcaacg gtccgggaaa attagtggaa gcattagcaa ttcctcaaga actttatggt
    19861 cagtctatag cagatagttc gttacgctta gtttttgaga aaaaaaagac accaaaaaag
    19921 atcaatgctt tgcctagaat cggtatccca aacaaaggtg tatggacaga aaagccgttg
    19981 cgttttgttg tgtcgggaaa tccttatatc tctttacaaa gaaaaaacca aatcgagaaa
    20041 gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat
    20101 aattgaaaaa caagtgacag actatgatgt agctttagat tgggatacaa aaaatcatac
    20161 gatcgagatc gttgttcgct tgtttgcaga aaataaagca caatttgaga ttgatgatgc
    20221 agaagggatc gtttcgcaag aaccaattat cgaatttgaa gacggcgtac ttctttttaa
    20281 tcctcagaaa tctgtttttg atgaacgcaa ttatctagca gtaatccctt atgaaggaaa
    20341 aaaagggttg gcaaaatcag tagctgattc tttagtggaa tatctaaatg aagtattagc
    20401 tcaagggcaa agcgatttgc ttgatttttt ggatgaagac gatgacgaag ctgtattcga
    20461 gcttcactgg gataatcagc aactagcaga attagtagaa gtaaaacagc aaaatgaagc
    20521 agatacttat ttaccgtacc caagttatta aggagtgaca gatatgaaat ggaatgaagt
    20581 aaaagtagaa actgaaagtg aagcagtcga agcagttgct aatattttga tggaggcagg
    20641 ggcgagtggt gtcgcgattg aagattcttt agatgttgaa cattttcagt cggatcaata
    20701 tggtgagatt ttagataaaa atgattttac atcgctgaaa gaaggcgcac tagtcatggc
    20761 ttattttccg gaaacgactt tcttaccgga aatcttgcct tttatcaaag atcgtatcac
    20821 acaattacct gagtttggtc tagctattgg aaaaaataaa gtaacagtta gtgaagtaga
    20881 ggaaagcaac tgggcaacag cctggaagaa atactatcat ccggtgcgta ttacgcgaat
    20941 gctgacggtt gcgccaagct gggaaaacta ccagacagaa gatcctctag aaaaaattat
    21001 cacactagat cctggtatgg catttggcac aggcactcat cccacgactt ccttgacatt
    21061 gacagcacta gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc
    21121 gggcgtgcta agtattgcag caaaatatct tggtgcaaaa gatgtctatg cctttgattt
    21181 agacgaagta gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa tagccaaaga
    21241 tgtccatgta tcagctaacg atcttttgac aggaatcgag attgaaagtg atgtaatcgt
    21301 tgcgaacatt ttagcagata tcatcttatt gatgatccca gatgcttggc gtttactgaa
    21361 gcaaacaggc acgctgatcg tttcaggaat cattgaagag aagaagcaaa tggttctgga
    21421 tgccatgtta gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag actggtatgc
    21481 aattattttg aaaaaaccag aggtggaata gatgcagcgg tattttttaa aagaatcata
    21541 tgaatcaaaa gactattttg ttttagaagg ggaaaactat caccacattg tacgggttat
    21601 gaggatggaa cccgaggatg aagttttttt ggcattcaat gatcaaatag ccattgttgc
    21661 agaaatcacg gagattactg aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa
    21721 tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc tatcctaaag gggaaaagct
    21781 ggattggatc gttcagaaat ccactgaatt aggagcgcaa gcttttcttg gtttcccggc
    21841 atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac aacggcttga
    21901 aaagattgct caagaggcag cggaacaatc ccatcggcag atcgtgccat cagtagagtt
    21961 attggaaacg aaacaagatt tatttgagcg gctagatgat tatgacgtgg tcttggttgc
    22021 ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac
    22081 aaaaccagat agtcgaatct tagctgtctt tggaccagaa ggtggattat cccgcgatga
    22141 agtcgattat tttgtagaac acaaagggat actttgtgga ttaggaccaa gaatccttcg
    22201 tacagaaaca gcacctcttt atttattaag tgctgctagc tatcaatggg aattaatgaa
    22261 tccttctttc agctaacgtt cttcattaat aaaagagtat cgacagaaag aattgaaaaa
    22321 atagtcaaca atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt
    22381 gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg
    22441 gacaattatc aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat
    22501 cgattcctgt gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc
    22561 aatacatggg tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg
    22621 cacatgacgg acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg
    22681 caggtatatt agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg
    22741 atgttgttga agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg
    22801 ttgctatgtt agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag
    22861 agcagctagc agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta
    22921 tcatggtcaa attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag
    22981 ataagcaaag aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc
    23041 tagggatcag ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa
    23101 aacagtatta ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg
    23161 taagtggtac tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa
    23221 tctatggacg gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc
    23281 agttcaacga aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt
    23341 gttatgctgt attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg
    23401 attatattgc catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc
    23461 cggcaggaaa tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat
    23521 tcggggttgc tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg
    23581 gtatgacgaa acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg
    23641 atgcttctga atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct
    23701 tcacaccaaa aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat
    23761 acagtatcca tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg
    23821 tgcagcttga ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt
    23881 catttggccc aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga
    23941 tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg
    24001 tggtcaaatg tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac
    24061 tgcaagaagc actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag
    24121 ggtatggaga agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag
    24181 aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag
    24241 aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact
    24301 tactaattcg tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata
    24361 tcactaaagg cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca
    24421 aaccaaatgt agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa
    24481 aagagtatga tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg
    24541 tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata
    24601 aagaaaaaat ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga
    24661 gtatcgtgga taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat
    24721 aggaggacag atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat
    24781 cagcaagaaa ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc
    24841 gatacgcaaa aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa
    24901 gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc
    24961 tctcaattca cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc
    25021 gcacgaccag aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc
    25081 ggcatcacag tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat
    25141 ggacctgtaa cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt
    25201 aaaaactatg aaacacgtcg cttaagttag actgataaac tctaacttaa gtggcttttt
    25261 tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa aagttaagct taaattatac
    25321 aagttatttt ttataggagg ttgctgaaga tgaaagaagt accagtagta gatatcaaaa
    25381 tcacagattt aaaaaaattg tatcaaaaag gatacaacat gttggaggag ttacgccatg
    25441 aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc acgatctacg
    25501 gatcatcagc agcaaaagta ttttatgatc caaggaattt caaacgaaaa ggtgctatgc
    25561 caaaattagt attgaagaca ctttttggtc aaggtggagt acagactttg gatggagctg
    25621 ctcatcacca tcggaaaaat atttttatgg atctaatgac accggaaaga atggaagatt
    25681 atcatcgtat cttagataag aatctgacac aagcacttga agcacagcat ggtcaatttg
    25741 aactatttga tttaagtaag atggttttct ttacatctat ttgcgagtgg gcaggtatca
    25801 atttatcagc gatttctaaa gatgaggtcg aaaaattagc agaatatcaa atctctatga
    25861 tcagcggaac gtttacttct ccgattgatc acataaaagg tgtcgaaaat cgcaaaaaat
    25921 cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg gctggaaaag
    25981 aaaatgtggc attatatgct tttgccaatg ccacagattt agatggtcag cttttacctc
    26041 tggaagtggc agctgttgaa ttattgaaca tcatccgtcc aacggttgct ctaactgtgt
    26101 gggcggcatt gatgggacat gcacttttca gtagaccaga tttgtatcag caattaaaaa
    26161 atgacttttc gacgttacaa gatccgttta ttcaagaaat gagacgctat tatcctttct
    26221 tcccaatgct acctgcgatt tcgctgaaag aagtagaagt agatggttat cgtataccag
    26281 aaggcagttg ggttatttta gatttatatg gaacagacca tgatgaacga acagttgaag
    26341 cgccagattc ctttatgatc aaacggtatg tagggaaagc aaaagatatt tcctataaag
    26401 aagaatatga gatgattgca caaggcgggg gaaattttcg acaaatgcat cgctgtgcag
    26461 gagaatggat cactcttcat agtctacgtg tgttttccga tcagctagta aataagtttg
    26521 agttctctgt acctgaacaa gattggacga ttccattcaa tcagtttccg acttatccga
    26581 atagccgagc gctactttac aagaattaga ttagcataaa cttatcgaaa aaaaggaaga
    26641 tcaaacagtg ctgatcttcc ttttttcata ttactataaa ttgataaggt caattgatca
    26701 aaatccacga aatgcttcat caatggtttg ataatcttca ttggataaat gcaaatctaa
    26761 agcttggaca ttgtcagata cttgctctgg acgcttagct ccaggtataa ctacagacac
    26821 tcgtggattc ttcatgtacc aagctaaaac aagttgcgca ggagtggctt ggtatctttt
    26881 agcaatgggg cgaagtacat cgacagcggt taaaatttct ctgaaacgtt ctccttggaa
    26941 atctggattt ctttttcgag gatctccttc accaaattgt ttttcctcac caagttcata
    27001 ttttcctgtc agcaaaccgg aagcaagagg aaaataggga acaaaactga ttttgttttt
    27061 ttcaagataa gggaataatt ctttttcagc ctgccgatgg atcaaactgt atttatcttc
    27121 tactacatcg acatatccat ctgcattcgc ttctttcaat tgttctaacg tgaagtttga
    27181 cacaccgact gctcggattt ttcctgcttc ttttaattcg tgcaatgtag caactgattc
    27241 attttttggt gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg
    27301 cagacgtttt agtgcatctt ccaccgattg tttgagaaac tcaggagaat tatcaaatgt
    27361 tcgcccttta tttggtacat gagcagcttt tgtagcgatg acgacacgtg agcgatcata
    27421 ttctttttcc ttcagtacct cgccaatcag ttcttcggaa cggccgtttc cgtaagcata
    27481 agcggtatcg atcaaattga tcccgctatt caaagctgta cgaactactt gttttcctgt
    27541 ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg gcatttgctc ctaatccaag
    27601 ctgttctgcg tagacttgtg aatgtccgat tcttatttct gccatagctg attcctcctt
    27661 tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa
    27721 tagtcagata tctcttgact ttgagactgg tttttagtag gttataagta atatcgaatg
    27781 tcaatgaaag agcgagtaag tttttgaaac tttacaagag aggatcatcg aggctgaaaa
    27841 tgatctaaag tttcattagc ctaaagacac tctggagatg gacgagtgaa aagccgtccc
    27901 gcgtacgagc gttattcgta agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt
    27961 atgaatttca ttcacccttg ttcatgatga acaagggtga tttttttatt tacatagcta
    28021 agaaagggag agaaaactat gagtttccaa agaccaaaag gaacaaatga tattttgcca
    28081 ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac gtttattatt cagagattat
    28141 cagtataatg aaatgcgtac accgattttt gaacactatg aagtgatatc ccgaagtgta
    28201 ggggatacaa cggatatcgt ttcaaaagag atgtatgatt tttatgacaa aggggaacgc
    28261 catgtgacgc ttcgaccaga aggtacagca ccaatcgttc gttcttttgt tgagcataaa
    28321 ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt atatgggacc aatgtttcgc
    28381 tacgaacgcc ctcaaaaagg acggttacgt caattccatc aaatcggagt agaagcattt
    28441 ggatcagaaa accctgcaac tgatgtggag agcatggtga tggcacttga tttcttcaaa
    28501 cagttgggga tcacgcaaat ccggctagtc atcaattcat taggtgataa agaaacgcgt
    28561 caagcctatc gtcaagcatt gatcgactat cttttacctt ttgaatcaga gctgagtgaa
    28621 gactctaaac gccgccttca tgaaaatcca ttacgcgtat tggacagtaa agacaaacga
    28681 gatcaaaaat ttgtggcaga agctccatct atcttagatt atctaagcga gcctgctcaa
    28741 aaacattttg ataccgttgt ttccatgctg gatgcattgg ctgttcctta tgaaatcgac
    28801 agtaatatgg ttcgcggact agattattac acacatacga tttttgaaat catgagtgac
    28861 gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg gacgttatga caatctagtg
    28921 gaagaactag gtggtccttc aacacctggc ttcggatttg caatgggtat cgagcgatta
    28981 ctcttgacta tggaggcaga ggaagtagtt atccctgcat ttaatgagtt ggatgcttac
    29041 gtggtcgcac tcggagacga aacaaatatc gaagcattga aagtggtcca agcgattcga
    29101 aactttggtt tttcagctga ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag
    29161 acagcagata aagcgaatgc caaattagta ttggtgatcg gtgaagatga gcttgctagt
    29221 caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc attagaagag
    29281 atatatgaac attttgatga agtctatgat gaaatgactt tattcgagta ggaggaaaaa
    29341 aataaatggc acaaagaacg atctattgcg gattagtttc cgaacaacaa ttagaacaaa
    29401 cagttacatt aaaaggatgg gtacaaaaaa gacgagacct tgggggagta atctttatcg
    29461 acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag
    29521 catgggaaat cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta
    29581 gaagacgcgg agaaaatgca gtaaatccta aaatggccac tggtgaattc gaagtgattg
    29641 caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc
    29701 aagcaatcag tgacgaaata cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa
    29761 tgactaaaaa catcaaatta agaaatgcga caacaaaagc gattcgtcat tatttagatg
    29821 ataatgactt tctcgatatc gaaacacctt acctagggaa atctacacca gaaggcgcaa
    29881 gagactacct agttccttca cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc
    29941 ctcaattgtt taaacaattg ctgatgaatg ctggattcga ccgttattat caaatcgttc
    30001 gttgtttccg cgatgaagat ttacgtggag accgtcagcc tgaatttacg caagtagata
    30061 ttgaaacaac gttcttaaca gcagaagaaa tccaaactta cacagaggga ttgattgcga
    30121 aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc atttcctcgt atgacctatg
    30181 atgaagcaat ggctcgctac ggcagcgaca aaccggatac acgttttgat atggaattga
    30241 tcgatctaag cgatacagtc aaagaagtag aattcaaagt cttccaaatg gctcttgaaa
    30301 acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag
    30361 acatggatca gttaggtcaa tatgtaggac aattcggagc taaggggctt gcatggctga
    30421 aagtagaaga agacggattg aaaggtccaa ttgctaaatt catgggagaa gcaacagaag
    30481 caatcattaa agcaacagat gcaaaaccag gtgacttgct gatgtttggc gcagataaaa
    30541 gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg tttaggaaaa gaattaggac
    30601 taattgatga atcgaaattc aatttcttat gggtaactga ttggccacaa tttgaatttt
    30661 cagaagaaga aggtcgatat gtctctgctc accatccatt cacgatgcca aaagaagaag
    30721 atatcccgtt attggctgaa gaccctgcaa aagtctatgc agaagcttat gatatcgtct
    30781 tgaacggtta tgaactaggc ggaggttcta tccgtattca tactcgtgag cttcaagaaa
    30841 aagtactaga aactctaggc tttacaaaag aacaaatgga agaacaattt ggtttcttgt
    30901 taacagcttt agattacgga ttccctccac atggcggtat tgcattagga ttggaccgtt
    30961 ttgcaatgtt attagccgga gaagataata ttcgtgaagt cattgccttt cctaaaaacg
    31021 gaaaagcagc agatgtgatg agtgatgcac caagtatggt ttcgccttta caattgttcg
    31081 aattgaatat tgatgtaacg aatatagaag aatagccgat aaaaaggtaa aaaggatttg
    31141 tcgaatacgc ttcttttgac aaccttgatt taattgaaaa acactttata atgaagccat
    31201 aacatataaa gcggggcttt tccatgaaaa aaacattcga aaattgggct tatcatgatt
    31261 acacgaccaa gctttcggta tctatcgttt atgcgttgat cgcatcagtc gcattgaatt
    31321 tcttttatca gccaggacat atttattcaa gtggtattac tggtctagct cagattttga
    31381 cgactttgtc aaaatcactg ataggattcg aagtaccggt atcaacaact ttgtatttac
    31441 tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt acaattttca
    31501 cttttatcac agtcactttg acctcccttt gtatccatat tttaccggaa acagtcctat
    31561 ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt gatgggacta ggtattgggc
    31621 tagcgttgag aaacggccta tcatcgggcg gtctagatat cgtgagtatc acgattcgta
    31681 aaaaaacagg aaagtcgatt ggttctattt cgatctactt caatgcattg atcgtttttg
    31741 ttgccggaat tctatttggc tggcaatata tgttttacag tacgttgtct attttcgtca
    31801 gtggaaaagt aactgatgtc gtttatacta aacaaaagaa gatgcaggta atgatcgtaa
    31861 cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga
    31921 tcatcaatga agcagaaggt gcttataagc atgataagca gacagtattg atgacgatcg
    31981 ttacacgata cgaactgcct ctacttagag aagcaatgaa agcttctgat ccacaagctt
    32041 ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt ctatgaggat ggcatttaga
    32101 tcgagtttga caaaaattca tgaaatactg ctgtagacag acgttcgttt gtctgcagtt
    32161 tttttctacc atagtagaat cagcaaagaa aaactgcttt tttatttgaa attttcttaa
    32221 agttgaaaaa aagcatgttt atttttttaa atttatggga tgatagaaaa aaatagtaga
    32281 gaaaggacac gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa
    32341 gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac
    32401 atcactgggt ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt
    32461 ctgccaacta gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt
    32521 tatttgatca cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg
    32581 caattttatg tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca
    32641 gcttcggctt atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg
    32701 gtcagcagct tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc
    32761 gatcgatttg ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa
    32821 aactatttgg tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa
    32881 tggatctttt atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg
    32941 tatacaccag gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct
    33001 atatggttgg gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc
    33061 ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt
    33121 ctttgcttct tctttttaga tgatcattac aatttcatct attacggagg aatgctccta
    33181 gtgagtctgt tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat
    33241 cgatggctga caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg
    33301 tatcagtttc cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt
    33361 ttgttgcata ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga
    33421 tatgtggagc gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga
    33481 tggttcagca aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt
    33541 gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa
    33601 aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat
    33661 tcagaaaaca ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag
    33721 gcacaaggga ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg
    33781 gatgctgcaa cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga
    33841 cgacaattat atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa
    33901 gacactgttc tgatcggact aggaacgaat ggctcattct cggaagctca gtttgatagt
    33961 gtgatggatg caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag
    34021 cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg
    34081 acgctgattg attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa
    34141 gtccatccaa atcctgaagg gatgacacat tacattcact tagtcagcga agccttgcta
    34201 ggaggaagtt caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa
    34261 acttcaggca aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac
    34321 attcatatag attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt
    34381 tttcgggtga gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat
    34441 acttctgcat ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca
    34501 gtaccattgt tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat
    34561 gtctgatctt tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg
    34621 aatctctgca gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa
    34681 tccgtctaat ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg
    34741 ttttgggtaa acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat
    34801 gatttctaag aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag
    34861 cttacttgaa atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc
    34921 caggtgttta ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca
    34981 gacgactaag tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt
    35041 gtggacagtc atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc
    35101 tagacgaggt tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag
    35161 tccaactgcg ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga
    35221 aaagtgagca agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat
    35281 cttgatatct tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct
    35341 ccaaaatgaa aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg
    35401 cttgtcaaac ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta
    35461 tgttttatcg atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg
    35521 cccgctatga gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc
    35581 atcgtacttg gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct
    35641 ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca
    35701 atgcttttcc agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga
    35761 ttttgaaatc gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag
    35821 aacttaaagg cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg
    35881 tatatcaagg tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt
    35941 tcggcgaacc aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga
    36001 tcgaacgacg aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca
    36061 aatatgaagt caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta
    36121 ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgcccc
    36181 ctttttgcgt tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta
    36241 taataattag aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg
    36301 gcatatcgga aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc
    36361 ttctatacta gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt
    36421 atatgatagg agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga
    36481 ttggaattta aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac
    36541 gagactttca gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct
    36601 agcagaagca tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt
    36661 tgaaccaatt gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc
    36721 ggtagccaag gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt
    36781 gattagccat ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt
    36841 aggcgggttg gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg
    36901 acatttgcat aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt
    36961 gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac
    37021 aggcctggag ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc
    37081 gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat
    37141 ctatatctat ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat
    37201 atatcctcgt attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa
    37261 acgaaaagta ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat
    37321 gacggaagag cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat
    37381 catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc
    37441 ttttatcaat gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag
    37501 cgggaagaca ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg
    37561 tgaaacttcc ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga
    37621 cgaagaaaca agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg
    37681 aaaaccagaa caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa
    37741 agtaacgttg accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac
    37801 agaagcagat cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat
    37861 cgttttacta ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga
    37921 gactgtttta cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa
    37981 agaaaaagca aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct
    38041 gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt
    38101 agcccaatgg acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc
    38161 tctaacggaa cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag
    38221 cttgaaaaaa agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc
    38281 tcaaaaagac gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg
    38341 attagtcgaa ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca
    38401 aaaaagtagt gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca
    38461 aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact
    38521 gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc
    38581 agaacaatta acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa
    38641 tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa
    38701 actgcaagaa gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga
    38761 aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa
    38821 aaaagcaact gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc
    38881 agaacagctg aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg
    38941 tccaggggag ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca
    39001 cgaacagcca aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc
    39061 tgctgtagaa agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca
    39121 acaacaatta tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca
    39181 agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac
    39241 aaaaacaact cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa
    39301 tgcaagaata gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc
    39361 tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat
    39421 caaaagttta gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg
    39481 acgacttgaa aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg
    39541 agtgcaattg ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca
    39601 aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga
    39661 aagccagtcg tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca
    39721 atataaagaa aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga
    39781 ccggctgcgt caattagaaa atatccagct aactgagaca ttcccagata cagaagagat
    39841 cagccaacag ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg
    39901 tttacaagaa caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca
    39961 cacaagccag acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa
    40021 cggagaaaat cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga
    40081 agtgttacaa gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga
    40141 gctagcggat accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga
    40201 tgatcaggca ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc
    40261 agcactttct ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc
    40321 aatcgatgca ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat
    40381 ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca
    40441 tgtaagagaa ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg
    40501 acaaagcaaa atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag
    40561 tattccagaa aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc
    40621 ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta
    40681 tatggttgat ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga
    40741 ggaacataaa tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct
    40801 atctcgtatc atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg
    40861 agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca
    40921 acctttgatt attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct
    40981 ttctattttg gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat
    41041 cgtaaaaaac atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa
    41101 taaacagtac cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact
    41161 cacgcatctt gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt
    41221 caaaggaaaa ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt
    41281 agagcagatg aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg
    41341 aatacaattt tccgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga
    41401 ttatttgctg caagcattga atgattttga ttttttcttt atgcattatc agtggggagt
    41461 tatccaaggg acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa
    41521 acaattgatt cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc
    41581 tttgtttaaa gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc
    41641 agtagaagag gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa
    41701 aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa gtcatctatt cgactgaccc
    41761 gaagcatgag gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa
    41821 acgagtactt gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg
    41881 tccattgcca agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg
    41941 tcagtttggt gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa
    42001 acatcaaccg aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt
    42061 gacagggata gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc
    42121 tttttataca ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac
    42181 aagccaagtg ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt
    42241 acctaaaaat caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag
    42301 tgaatcgttc caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct
    42361 tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa
    42421 gatgttaggt cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac
    42481 actacagaca attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt
    42541 gatcgtagcg cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc
    42601 ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa
    42661 agacacagat atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca
    42721 gtcgcaaaaa attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac
    42781 gaaaactgca caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac
    42841 accaatcgag aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt
    42901 tttcccgact aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg
    42961 accgtttatt ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga
    43021 gatgaatcta tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg
    43081 tcaaatgcgt gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg
    43141 gatacttgct ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga
    43201 agattaccaa ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa
    43261 agagaacaaa cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca
    43321 agaagagtta gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca
    43381 agatcggtta agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc
    43441 cttaaaggct gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga
    43501 cctttggtgg aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca
    43561 aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga
    43621 ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca
    43681 gttgacgaaa ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga
    43741 gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg
    43801 tttatttgat ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc
    43861 tatcattttt ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac
    43921 agctgatcaa aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct
    43981 cgatggttga accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg
    44041 taattatcgc caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagctagc
    44101 tgaaaaatct ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg
    44161 gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc
    44221 ccatttgtga taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg
    44281 acctcctcat gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg
    44341 ccatcttctt ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt
    44401 gctcgcatat attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta
    44461 tgatggcgga accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact
    44521 cttttgaccg cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac
    44581 acaactgatt taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc
    44641 cttgactatt gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac
    44701 tatatttcta tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat
    44761 cgtgaaacac gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat
    44821 ttattttaat gcaatctctg tggaattttc gaaagattta tttttttaat acagtctcaa
    44881 tataactatt caaacataaa ataacgttat ttgatttatt tgtttatagt aaattttatt
    44941 tagtaatcat tgctttaaaa tactacgctt atataacaga gaaaataagc gttataataa
    45001 agaacagtat tatttaaaat gacagaaaac attactatta aagaaataaa aaacataaaa
    45061 aatcttaaga aatcggtttc atttagcttt tgagataaaa taatagtaca gttttatacg
    45121 ctgtttcata tgtttagaaa atttgaatta gatttgaaaa ctctaatttt taaatgataa
    45181 aaaaagcgag aatgctggtt ttactgtata ttaaccttag ataactttca tttttttaaa
    45241 aaaattatga aaaaaaggta gctatttaaa agttgattat gtataataga aacagatggg
    45301 agattattat gatcttctgt tacaaaaaag tgattcttat cacgaaaagg agtggtgcac
    45361 gttgttaaca ttatatactt ccccaagttg tacttcctgc agaaaggctc gtgcgtggtt
    45421 gcaagaacat cagattccat ttgttgaacg taatattttt tcagaaccat tgaacagttc
    45481 tgaattaaaa gcgattttac aaatgacaga agacggaaca gaagaaatca tctcgacacg
    45541 ttccaaagta ttccaaaaac tgaacatgga tttagatgat ctgccgttac aagaattatt
    45601 ggaacttgtg caaaacaatc ctggattgct tcgccgtcca atcatgattg atgataaacg
    45661 tctgcaagtt ggttttaatg aagatgaaat tcgtcggttc ctaccgcgcg atgtacgaca
    45721 attagaatta cgtcaagcac aattgatggc tggattatag aaggataata cgaaacttgg
    45781 aaaagactat tacaaagata gtttttccaa gtttttttgt ttttacaaaa agtattcttg
    45841 attagatgta taaaaaggta gtagaagatg atgattgcac gaaaaataga gagggaaaat
    45901 tagctgaaaa gtcggctata tagtattcgt ctgccaaaaa tgttatagtt aaataagata
    45961 gcttgaaagg ttgaaaaatc cgctggtttt attaaattcg cttaacagcg agattttttt
    46021 tctttacatt taagctgatt tcgctacaat gaatctatat taaacattgt ggcaaagtga
    46081 ggtgtagcaa aatggaaatg gaacatatta atgaaaatac gatccgtgta ctaattggtc
    46141 atgaagattt agaggaacgg ggcgtttcat ttttggattt acttggcaat cacaaagaaa
    46201 tcgaaaattt cttttatagt attttggaag aagttgatat cgaagaggag ttccgaggaa
    46261 gcgaagctgt cactttccaa gttttgccaa aaggagacgg attagaactg tttatcagta
    46321 aaaatcttcc ggatgaagaa atttctcaat cagaagattc tactgttgat tcagaagatg
    46381 tgagtgactt tcttcgtcag cagattattg gcagcgatcc ggaggaagaa ttatctggaa
    46441 gcgccctaga agaacaggaa agacaagcaa cttttgcttt tgaagatttt gaacaagtga
    46501 tccaacttgc aacagatgtc gaactagagt ctgcttggac tgatttgtat ttattcgaag
    46561 agcgctatta cctagtcgtt cacttctgga tggaaaatct caaccaagcg gatgtagaga
    46621 atcagattgc tcgaattttg gagtttgctg aaaagagcga tcgtacacca gaaacattga
    46681 atgaatatgg acaatgtctg atggcgcgta atgctatcga gcgaacgaaa ttttattttg
    46741 tataaatcag cattatagta agagactgtg acaaaaattg tgtcgcagtc tttttttcgt
    46801 ataaatgtgt tcaaaagctt gcatctgcgg taacaagccc aaccaaacga aaaatattgg
    46861 gaactatttt tgtttagctg ttcttattac ttgatgcaga atagcttttt cacgccctct
    46921 tttttcgtgt aaataaattc ttagacgtaa ataagttcag agggagaaaa atgctgcttg
    46981 caatgaatga atataataag tgctgttttg ctaaacatgc agaaaaagga aagaaatatt
    47041 attgtcgtgg ctgtcgaaaa agagtgattt tgaaaaaggg aaagaaaaaa tgtgctcatt
    47101 ttgcccatca aaaatcagat aactgttcgg tctttagtga aagagaatca aaagagcact
    47161 tacagttaaa ggaatgcttc atggattggc tgggacagtc agtagaacca gtatttttgg
    47221 aagcctattt gccgaggttg cggcaaaggc cggatatttt gttggcgaat ctagcaatag
    47281 aaatccaatg ttctagactg tctcaccaga gatttattga aagaacccaa aattatttga
    47341 ataacagtta ccaagtatgg tggatacttg gtaattcttt tcttggacaa tcgcaattct
    47401 cattgataga aaaaagttgc tgctactata atagaaaaag aggagtgcat tgctggaaag
    47461 ctgatctcaa gaaacaaaag ctttatttgt atcatcatat tacagagact gtatcagggc
    47521 atatttcctt ttgcagtagc tgctggactt tttctagtag ggaccttaaa gaaatattca
    47581 ctagtaacga aattaaaatg aaccaaatga aaaaaataga aaaagtgagt gaagatggga
    47641 agaaatggct gacacgccaa cttattcaca agcaaaagaa tacagtatcc atacaagaac
    47701 aatgctactt gcgtcataaa catcttttgc atctatcatc gtggatctat caaaaaagtc
    47761 ggtttttctt ttatttacaa gaacaggttt ttctttatcg aatgttgtac gaagaagcct
    47821 tggaaaacca gaaagttcct gatttcaact catggctttg ccaaataaaa gaacataaaa
    47881 gggaatggct ttttcctatg attgatgaag agatggtcta tcagcagttt tttaacgaat
    47941 gcatacactt gtcttctttg aaaaaatagt aagtataaaa ataggctagg aatcggtttt
    48001 ctatgtaaaa caagatttag ttatgctaaa attgatggaa gcagacacat gaatgagtag
    48061 aaaagttaga tgctgtgaca tgtggatgtt agactatgat ctattttgat agatagagct
    48121 aagggaagaa aaggagtgga tttcatggaa gtaaagcagt tgccaaaacg agaagaattg
    48181 cctgaaaatt taacttggga cttgaccaag atcttttcaa gcgaccaaga gtttgatgag
    48241 aaatatttgg aattatcaga agagttaaaa caatctgaaa aacacaaagg aacacttgat
    48301 caaggcgctt ctcaattttt aaatgcgatt gaattcgtat tgagggttta tcgccaaact
    48361 gaagtcattt atgtatatgc gcaccttaaa aacgatcaag acactggaaa tacagattac
    48421 caagcgcttt atgcaagagc aagcagtctg ttttcgaaag ttagtgaagc cgtttcctgg
    48481 tttgaaccag aaatattgca attgtcagat gaccagattt ggcaatattt caaagaagaa
    48541 ccaaaattgg aagtctatcg ccattatatc cagcaaatag tagataatcg agcccatgtc
    48601 ttatctgctg agcaggaatc tcttcttgct ggagcaggtg aaatctttga tgcttcaagt
    48661 gatacatttg ctgttttgaa taatgcagat ctagtttttc caacgattga aggagaaaat
    48721 ggtgaaatag tccaattatc tcatggcgtg tatggtcagt tgctagaaag cacggatcga
    48781 agggtgcgcg aagcagcatt taaggggttg tacagtgttt acgaacaatt tagaaataca
    48841 tttgcttcta ctttaggcac acatataaaa ggacataatt ttaaagcgaa agtccgtaat
    48901 tacagctctg ccagagaagc gtctttgagc aataatcata ttcctgaaag tgtatacgat
    48961 actttggtag acgtggtaaa caagcatttg cctttgttac atcgatacat ggaattacgg
    49021 aaacgtttat tagaagtgga aaaactgcac atgtatgatc tttatacacc ggtcttaggg
    49081 gaagctccaa ttacctttac gtacgaagaa gcaaaagaaa aagctttaga agcactgaaa
    49141 ccaatgggtg aagaatacat ggccatcgta gaaaaagcat tctctgaacg ttggatcgat
    49201 gttgtcgaaa ataaagggaa acgaagcggt gcttattctt cgggaagcta tgacacaaat
    49261 ccatatattt tattgaattg gcatgatacg ctggatcagc tatttacgct tgtccacgaa
    49321 atgggacata gtgttcatag ttatttcact cgttcgaacc agccttatgt gtacggcgac
    49381 tactccatct ttttagcaga aattgcttcg acaacgaatg aaaatatcct aacggagtat
    49441 ttattggaaa cagaaaaaga ccctcgtgta cgggcttatg tactcaacca ttacttggat
    49501 gggtttaaag gaacagtttt ccgccagaca caattcgctg aattcgagca tttcatgcat
    49561 acggaagatg aaaaaggtgt gccattgact agtgaatacc taagtgatag ttatggtaaa
    49621 ttgaatgcaa aatattatgg tccagcagtc gaagaagacc cagaaattaa attcgaatgg
    49681 tcacggatac cgcattttta ttataattac tatgttttcc aatactcgac tggcttttct
    49741 gctgcttcag cactggcgaa gaaaatacta aaccaggaac cagaggcact agagaactac
    49801 ttggcttacc taaaagcagg taacagcgat tatcctgtgg aagtaatgaa aaaagcagga
    49861 gttgatatga cacaggctgc atatattgaa gatgcaatgt cgatgtttga acaacgtcta
    49921 aatgaattag aagaattgat cgatcgttta taaggctttc tatttttgat tttctcgaat
    49981 agaaacgaaa aaaatacgtc ggctctcttg taagggagtc gacgtatttt tt
//
LOCUS       DASHWH010000014        49988 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.014, whole genome shotgun sequence.
ACCESSION   DASHWH010000014 DASHWH010000000
VERSION     DASHWH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.014"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U2R41_000818"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U2R41_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM8888248.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="U2R41_000819"
     CDS             complement(322..891)
                     /locus_tag="U2R41_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEM8888249.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="U2R41_000820"
     CDS             1077..1979
                     /locus_tag="U2R41_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8888250.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U2R41_000821"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U2R41_000821"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEM8888251.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U2R41_000822"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U2R41_000822"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEM8888252.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U2R41_000823"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U2R41_000823"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEM8888253.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="U2R41_000824"
     CDS             8547..8732
                     /locus_tag="U2R41_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEM8888254.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="U2R41_000825"
     CDS             complement(8794..9990)
                     /locus_tag="U2R41_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8888255.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="U2R41_000826"
     CDS             complement(10160..11596)
                     /locus_tag="U2R41_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM8888256.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="U2R41_000827"
     CDS             11889..12698
                     /locus_tag="U2R41_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8888257.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="U2R41_000828"
     CDS             complement(12884..15037)
                     /locus_tag="U2R41_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEM8888258.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15432..16280)
                     /locus_tag="U2R41_000829"
     CDS             complement(15432..16280)
                     /locus_tag="U2R41_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8888259.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16504..18444)
                     /gene="thrS"
                     /locus_tag="U2R41_000830"
     CDS             complement(16504..18444)
                     /gene="thrS"
                     /locus_tag="U2R41_000830"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEM8888260.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18781..18960)
                     /locus_tag="U2R41_000831"
     CDS             complement(18781..18960)
                     /locus_tag="U2R41_000831"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEM8888261.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(19007..20830)
                     /gene="cadA"
                     /locus_tag="U2R41_000832"
     CDS             complement(19007..20830)
                     /gene="cadA"
                     /locus_tag="U2R41_000832"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM8888262.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21108..23387
                     /locus_tag="U2R41_000833"
     CDS             21108..23387
                     /locus_tag="U2R41_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8888263.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23480..24127)
                     /locus_tag="U2R41_000834"
     CDS             complement(23480..24127)
                     /locus_tag="U2R41_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888264.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24306..25025)
                     /locus_tag="U2R41_000835"
     CDS             complement(24306..25025)
                     /locus_tag="U2R41_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888265.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25156..26307)
                     /locus_tag="U2R41_000836"
     CDS             complement(25156..26307)
                     /locus_tag="U2R41_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEM8888266.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26524..27264)
                     /locus_tag="U2R41_000837"
     CDS             complement(26524..27264)
                     /locus_tag="U2R41_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8888267.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27268..27591)
                     /gene="rsfS"
                     /locus_tag="U2R41_000838"
     CDS             complement(27268..27591)
                     /gene="rsfS"
                     /locus_tag="U2R41_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEM8888268.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27648..28241)
                     /gene="yqeK"
                     /locus_tag="U2R41_000839"
     CDS             complement(27648..28241)
                     /gene="yqeK"
                     /locus_tag="U2R41_000839"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEM8888269.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28225..28875)
                     /locus_tag="U2R41_000840"
     CDS             complement(28225..28875)
                     /locus_tag="U2R41_000840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEM8888270.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28885..29196)
                     /gene="yhbY"
                     /locus_tag="U2R41_000841"
     CDS             complement(28885..29196)
                     /gene="yhbY"
                     /locus_tag="U2R41_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEM8888271.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29212..30321)
                     /gene="yqeH"
                     /locus_tag="U2R41_000842"
     CDS             complement(29212..30321)
                     /gene="yqeH"
                     /locus_tag="U2R41_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEM8888272.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30318..30845)
                     /locus_tag="U2R41_000843"
     CDS             complement(30318..30845)
                     /locus_tag="U2R41_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEM8888273.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(31025..31810)
                     /locus_tag="U2R41_000844"
     CDS             complement(31025..31810)
                     /locus_tag="U2R41_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEM8888274.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31803..32672)
                     /locus_tag="U2R41_000845"
     CDS             complement(31803..32672)
                     /locus_tag="U2R41_000845"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEM8888275.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32673..34052)
                     /locus_tag="U2R41_000846"
     CDS             complement(32673..34052)
                     /locus_tag="U2R41_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEM8888276.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(34060..34479)
                     /gene="fabZ"
                     /locus_tag="U2R41_000847"
     CDS             complement(34060..34479)
                     /gene="fabZ"
                     /locus_tag="U2R41_000847"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM8888277.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34504..34980)
                     /gene="accB"
                     /locus_tag="U2R41_000848"
     CDS             complement(34504..34980)
                     /gene="accB"
                     /locus_tag="U2R41_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEM8888278.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34983..36218)
                     /gene="fabF"
                     /locus_tag="U2R41_000849"
     CDS             complement(34983..36218)
                     /gene="fabF"
                     /locus_tag="U2R41_000849"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEM8888279.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36237..36974)
                     /gene="fabG"
                     /locus_tag="U2R41_000850"
     CDS             complement(36237..36974)
                     /gene="fabG"
                     /locus_tag="U2R41_000850"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEM8888280.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36986..37903)
                     /gene="fabD"
                     /locus_tag="U2R41_000851"
     CDS             complement(36986..37903)
                     /gene="fabD"
                     /locus_tag="U2R41_000851"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEM8888281.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(38010..38234)
                     /locus_tag="U2R41_000852"
     CDS             complement(38010..38234)
                     /locus_tag="U2R41_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM8888282.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38287..39249)
                     /locus_tag="U2R41_000853"
     CDS             complement(38287..39249)
                     /locus_tag="U2R41_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEM8888283.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39259..39699)
                     /locus_tag="U2R41_000854"
     CDS             complement(39259..39699)
                     /locus_tag="U2R41_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8888284.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            40005..40970
                     /locus_tag="U2R41_000855"
     CDS             40005..40970
                     /locus_tag="U2R41_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM8888285.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(41027..41257)
                     /locus_tag="U2R41_000856"
     CDS             complement(41027..41257)
                     /locus_tag="U2R41_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEM8888286.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41343..42230)
                     /locus_tag="U2R41_000857"
     CDS             complement(41343..42230)
                     /locus_tag="U2R41_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEM8888287.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42319..43542)
                     /locus_tag="U2R41_000858"
     CDS             complement(42319..43542)
                     /locus_tag="U2R41_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8888288.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43559..44605)
                     /locus_tag="U2R41_000859"
     CDS             complement(43559..44605)
                     /locus_tag="U2R41_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8888289.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(44769..46496)
                     /gene="ptsP"
                     /locus_tag="U2R41_000860"
     CDS             complement(44769..46496)
                     /gene="ptsP"
                     /locus_tag="U2R41_000860"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEM8888290.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46496..46762)
                     /locus_tag="U2R41_000861"
     CDS             complement(46496..46762)
                     /locus_tag="U2R41_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEM8888291.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47241..49475
                     /locus_tag="U2R41_000862"
     CDS             47241..49475
                     /locus_tag="U2R41_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8888292.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49623..49943
                     /locus_tag="U2R41_000863"
     CDS             49623..49943
                     /locus_tag="U2R41_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM8888293.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14285 a        10730 c         8735 g        16238 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaacaa gaagtattta atattttttt
    15121 cataaaatct ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc
    15181 caaaaacttc ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc
    15241 aagataaaat caactttttg tttcacagca atcacttaaa gttacatatc caactatttt
    15301 tttcattaaa aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt
    15361 gatggatcat ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa
    15421 tagtaaggtt tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc
    15481 actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta
    15541 gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta
    15601 gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac
    15661 gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt
    15721 ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg
    15781 agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc
    15841 agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc
    15901 gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct
    15961 tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt
    16021 gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt
    16081 ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca
    16141 ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta
    16201 ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat catactttca
    16261 atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata
    16321 ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc
    16381 aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga
    16441 atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac
    16501 ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt
    16561 caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc
    16621 caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat
    16681 cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa
    16741 acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt
    16801 tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc
    16861 gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt
    16921 ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg
    16981 tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg
    17041 tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc
    17101 ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt
    17161 attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata
    17221 tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac
    17281 aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc
    17341 tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata
    17401 agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac
    17461 aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca
    17521 gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta
    17581 acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc
    17641 ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag
    17701 ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc
    17761 tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt
    17821 tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc
    17881 agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt
    17941 aatcacttct tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa
    18001 aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac
    18061 gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc
    18121 attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt
    18181 tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc
    18241 aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg
    18301 agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt
    18361 tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc
    18421 atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga
    18481 tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa
    18541 agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc
    18601 gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa
    18661 agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat
    18721 caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac
    18781 ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac
    18841 atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat
    18901 catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat
    18961 aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg
    19021 tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa
    19081 tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg
    19141 tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg
    19201 ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt
    19261 tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa
    19321 taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa
    19381 caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac
    19441 gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat
    19501 ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg
    19561 ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg
    19621 ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc
    19681 caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca
    19741 gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga
    19801 tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc
    19861 cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat
    19921 caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga
    19981 tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca
    20041 gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc
    20101 accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag
    20161 ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt
    20221 gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta
    20281 aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca
    20341 aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt
    20401 cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca
    20461 attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt
    20521 ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca
    20581 atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg
    20641 ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca
    20701 agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa
    20761 aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga
    20821 aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc
    20881 agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt
    20941 aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat
    21001 attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta
    21061 tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac
    21121 aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc
    21181 tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta
    21241 atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc
    21301 aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc
    21361 aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc
    21421 gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa
    21481 tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg
    21541 cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta
    21601 aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact
    21661 ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga
    21721 tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag
    21781 gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc
    21841 ataatcgcaa gtggctcgag gctgaaaatg ttcttctatt ttcacccaac catttgtttt
    21901 ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat
    21961 tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag
    22021 aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct
    22081 tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg
    22141 atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag
    22201 aaacaaatga acaattacct ttacatcata gactttctct attacaaacg aagctcctga
    22261 aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg
    22321 aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg
    22381 agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc
    22441 atcgagggat catgaattat caatttgtca atataacgaa acaatatctc cactttctac
    22501 aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac
    22561 atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac
    22621 tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata
    22681 tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt
    22741 tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa
    22801 cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa
    22861 cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag
    22921 aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa
    22981 cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc
    23041 ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc
    23101 aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga
    23161 agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt
    23221 tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca
    23281 ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt
    23341 tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac
    23401 tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa
    23461 ttcaatcatt taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta
    23521 atttgacaag cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa
    23581 ataccttgct atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt
    23641 caatgaattg gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct
    23701 ctgatacacg aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct
    23761 tctcatttct ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta
    23821 gaaagaaagc ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac
    23881 gaaaaagcag cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca
    23941 tacactctaa ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt
    24001 caacgccgaa tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta
    24061 actcttctac tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg
    24121 cttccatatt cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat
    24181 gctctcatca taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac
    24241 aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg
    24301 gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc
    24361 agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt
    24421 tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct
    24481 tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc
    24541 attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct
    24601 tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca
    24661 cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca
    24721 ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc
    24781 aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata
    24841 tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca
    24901 tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa
    24961 atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt
    25021 cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc
    25081 attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca
    25141 tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt
    25201 gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt
    25261 ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc
    25321 atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt
    25381 tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt
    25441 tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat
    25501 gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga
    25561 taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga
    25621 aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc
    25681 atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc
    25741 tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt
    25801 tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata
    25861 cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc
    25921 ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta
    25981 atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa
    26041 gatttttact gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag
    26101 ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat
    26161 gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc
    26221 tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta
    26281 ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag
    26341 aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat
    26401 tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg
    26461 ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa
    26521 ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc
    26581 tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata
    26641 actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga
    26701 cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc
    26761 tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg
    26821 aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc
    26881 taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca
    26941 cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat
    27001 attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat
    27061 caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac
    27121 cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg
    27181 acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat
    27241 gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg
    27301 agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg
    27361 tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg
    27421 ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat
    27481 ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg
    27541 aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt
    27601 taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct
    27661 acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa
    27721 tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg
    27781 gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa
    27841 atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga
    27901 agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca
    27961 tgccagattg cattattata attcaataac tccggatcaa aaccgtctct tacgattacc
    28021 attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta
    28081 gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca
    28141 agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct
    28201 ctagaataag cagtatattc attactatag tccatattca tacaaccctt cattttgaat
    28261 ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg
    28321 acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg
    28381 agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc
    28441 atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata
    28501 atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc
    28561 accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc
    28621 caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg
    28681 ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat
    28741 cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt
    28801 cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc
    28861 ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata
    28921 cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc
    28981 gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg
    29041 aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat
    29101 tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct
    29161 acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa
    29221 tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc
    29281 tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa
    29341 ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa
    29401 tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt
    29461 tacatcattt gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct
    29521 tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat
    29581 ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg
    29641 gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat
    29701 tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt
    29761 atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata
    29821 gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga
    29881 tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc
    29941 cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt
    30001 cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc
    30061 attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt
    30121 cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg
    30181 taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt
    30241 ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat
    30301 acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct
    30361 aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct
    30421 gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata
    30481 tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc
    30541 tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca
    30601 aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg
    30661 attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta
    30721 ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt
    30781 aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa
    30841 aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg
    30901 tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag
    30961 catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa
    31021 acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc
    31081 gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc
    31141 gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc
    31201 gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt
    31261 tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca
    31321 tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga
    31381 tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct
    31441 ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg
    31501 taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc
    31561 cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt
    31621 gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt
    31681 cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa
    31741 aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg
    31801 tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc
    31861 tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca
    31921 gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca
    31981 gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg
    32041 gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg
    32101 ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct
    32161 tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct
    32221 cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa
    32281 ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg
    32341 actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga
    32401 aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca
    32461 ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat
    32521 actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc
    32581 cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc
    32641 cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt
    32701 gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact
    32761 gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata
    32821 aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg
    32881 attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca
    32941 ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga
    33001 gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct
    33061 tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt
    33121 ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga
    33181 gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg
    33241 gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt
    33301 tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt
    33361 aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga
    33421 acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat
    33481 gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga
    33541 actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca
    33601 attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga
    33661 atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca
    33721 tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca catggaggca
    33781 aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg
    33841 attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca
    33901 atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat
    33961 atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca
    34021 ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa
    34081 ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga
    34141 agttttcact ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt
    34201 acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag
    34261 agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat
    34321 aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt
    34381 agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata
    34441 acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt
    34501 tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc
    34561 tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat
    34621 cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc
    34681 tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat
    34741 tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc
    34801 aggtatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat
    34861 gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa
    34921 ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa
    34981 aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa
    35041 ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca
    35101 atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc
    35161 agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat
    35221 ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt
    35281 gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt
    35341 tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca
    35401 tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct
    35461 ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct
    35521 gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta
    35581 ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa
    35641 atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg
    35701 aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata
    35761 ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt
    35821 acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg
    35881 attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt
    35941 ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc
    36001 gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct
    36061 ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg
    36121 atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt
    36181 gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc cttttcttat
    36241 ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt
    36301 gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt
    36361 tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg
    36421 aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat
    36481 tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca
    36541 atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt
    36601 ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg
    36661 aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact
    36721 aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca
    36781 taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct
    36841 atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca
    36901 aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt
    36961 ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct
    37021 tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata
    37081 cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac
    37141 tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt
    37201 tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa
    37261 tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag
    37321 gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag
    37381 cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg
    37441 caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg
    37501 gcgcattcat cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag
    37561 cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta
    37621 atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct
    37681 tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct
    37741 gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac
    37801 ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt
    37861 gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat
    37921 ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa
    37981 tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact
    38041 aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca
    38101 atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca
    38161 gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt
    38221 ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt tttataattt
    38281 aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa
    38341 tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa
    38401 gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc
    38461 ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa
    38521 aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg
    38581 tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc
    38641 ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt
    38701 accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat
    38761 cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt
    38821 agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc
    38881 agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc
    38941 atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg
    39001 cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat
    39061 caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg
    39121 ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag
    39181 atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa
    39241 ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc
    39301 atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc
    39361 acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt
    39421 tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt
    39481 gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt
    39541 tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc
    39601 tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact
    39661 aacaagataa tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag
    39721 tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct
    39781 taaaagatca tttttttact atatgagctt tttttaagat tttactcatt taaaacaact
    39841 gataaatcag catttataat ttcttttatt agaaaaatca tctatctcac atattttgat
    39901 tatcaaagta ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca
    39961 aaatttgtta tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa
    40021 tgaaaactct ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca
    40081 tctatgttgg taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata
    40141 aacagcattc aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg
    40201 tagtagacac ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc
    40261 ctttttataa ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata
    40321 agggatttgt attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata
    40381 aaaaaatgcc gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg
    40441 aaattagttt gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac
    40501 agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact
    40561 tgaaaaaaag acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc
    40621 aaaaagatat cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt
    40681 taagaacacc atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc
    40741 actacctcgt ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc
    40801 ctgacttcta ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag
    40861 aagaaatcga tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt
    40921 ttgaaattga actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg
    40981 gcttctttta ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt
    41041 catagatttc catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg
    41101 tttcagttaa ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc
    41161 cttctttttc aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca
    41221 gtcgttttat gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt
    41281 ctgttatatt gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt
    41341 ttttatgatt ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg
    41401 gttccgtcat tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta
    41461 gttgctggca atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg
    41521 ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa
    41581 ttgcttaaag accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa
    41641 tcaagtccgt ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca
    41701 atcatgatct gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta
    41761 aatgtcgtac caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca
    41821 ccgaccatga tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca
    41881 tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt
    41941 gtacttgtag tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac
    42001 tgtccatagt agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta
    42061 ctttgttctg ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga
    42121 tcagcttttg cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg
    42181 cgtgcgacag cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc
    42241 tcctaaaaat caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc
    42301 ataaaaaatg tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat
    42361 cgagtcttct tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata
    42421 aaattcgatc atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa
    42481 aacttttgga tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc
    42541 ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc
    42601 atttccttcc acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg
    42661 tgtttctgaa gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc
    42721 attgttgtcc acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa
    42781 ttcctctaac tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata
    42841 agcggagatg acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat
    42901 cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt
    42961 gctgatatcc tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg
    43021 aatcacgcga agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact
    43081 tggacaaact acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga
    43141 ttgcctgatc acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta
    43201 agtatggatc agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc
    43261 gaattctgta tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata
    43321 ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat
    43381 tcgaatcaca tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac
    43441 ttcatgccca tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct
    43501 gacttgcgga aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact
    43561 agtccttgac ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt
    43621 cgtatacatt tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat
    43681 aaccggcttg tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa
    43741 attcttcgtt tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca
    43801 ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc
    43861 cttctgtctc ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta
    43921 tgatctctcc acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa
    43981 ttttccggat gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt
    44041 attcgggcat cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca
    44101 aaccgacaca aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt
    44161 gttctttcat tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa
    44221 tcggcacacg gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca
    44281 gatagtccgc tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt
    44341 tcgatccgat aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt
    44401 tttttccttc tcttctggct ttattgacca tattatgact attgattccg tatgtattga
    44461 tgtgcaggat gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca
    44521 gagcacgttt ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac
    44581 tttcaaaata taataatacc ttcaccttac gtcccccttc acttatatag tataacatag
    44641 cctacaaact gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt
    44701 tttcttaaaa aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga
    44761 tacgttgttt attttacata ttcatgaaca agttcaacga cttcttccat cgtatcgcat
    44821 tctttcaatg cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa
    44881 ctacgcgttt taagaataga tgtcgcactc attgagaact catctagacc catacctact
    44941 agcaatggaa cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct
    45001 tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt
    45061 tggtacaagt aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa
    45121 tcgttcgtac ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct
    45181 gctgctggaa tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca
    45241 cttatcaatt tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt
    45301 gtagctacca tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca
    45361 cgcatttgtg tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga
    45421 tatcctaaga atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca
    45481 ccgatgtcca ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg
    45541 taagcttcat attggtcatc ttctgttggg aagtctggag aatccatata caggaattca
    45601 gtacggtaca gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt
    45661 gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt
    45721 tttaattttt cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca
    45781 gctttttgtt catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa
    45841 atatcgcctt ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct
    45901 aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct
    45961 tttacaaaat tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg
    46021 accacttctt cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa
    46081 atacgtttag caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc
    46141 attgcttcga acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt
    46201 acgctgttgt ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc
    46261 atcaaatggg catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca
    46321 cgaatttgct gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca
    46381 gccgcagtat cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa
    46441 gcttttgcaa cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt
    46501 cagataagcc ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag
    46561 caccttctgc agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca
    46621 tgattgattt caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt
    46681 tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta
    46741 cgtgaaattc tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt
    46801 gagggttttc ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa
    46861 agattaacat tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt
    46921 caaattattc cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt
    46981 tcctttttct tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt
    47041 tgttttcata tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa
    47101 aggtcaggaa tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact
    47161 aggggttatt cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg
    47221 tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc
    47281 acttgtatgc cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc
    47341 aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg
    47401 gatttggcag tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa
    47461 attatgagca aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc
    47521 cacctcaagg caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag
    47581 cacgacaagg tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg
    47641 aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta
    47701 aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat
    47761 taatggacaa agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac
    47821 gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg
    47881 tgatcctatt catagatgaa gttcacgaaa tagtcggtgc tggttctgct ggtgatggaa
    47941 atatggatgc tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg
    48001 gtgctactac cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta
    48061 tgcaacctgt acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt
    48121 tgcaaaaacg ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag
    48181 ccgcaacttt atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt
    48241 tgctagatga aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa
    48301 tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt
    48361 tcgaaaaagc agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc
    48421 agctcactga agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac
    48481 aacggacagg tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact
    48541 tagcagatga cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga
    48601 aagcgattcg ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct
    48661 tatttgttgg accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac
    48721 tattcggttc acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata
    48781 gtgtctctaa attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat
    48841 taacagaaaa agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag
    48901 ctcatccaga tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg
    48961 cacaaggaag aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa
    49021 ctggtaaagt agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg
    49081 tattgaacca attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca
    49141 tcgaattcag tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg
    49201 atgtcaacca aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag
    49261 aaaaattagt tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga
    49321 tccaagaaca aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg
    49381 agttgaaagc aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt
    49441 tggctaaaga atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt
    49501 atttctttga gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt
    49561 ataaaaaaga aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg
    49621 ccatgaaatt ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa
    49681 acactgatgc ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg
    49741 aagcccctga acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag
    49801 aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg
    49861 atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat
    49921 taggcgctac attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca
    49981 aaagtaaa
//
LOCUS       DASHWH010000015        49819 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.015, whole genome shotgun sequence.
ACCESSION   DASHWH010000015 DASHWH010000000
VERSION     DASHWH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.015"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..243)
                     /locus_tag="U2R41_000864"
     CDS             complement(<1..243)
                     /locus_tag="U2R41_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEM8888294.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(358..936)
                     /locus_tag="U2R41_000865"
     CDS             complement(358..936)
                     /locus_tag="U2R41_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8888295.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1013..1543)
                     /locus_tag="U2R41_000866"
     CDS             complement(1013..1543)
                     /locus_tag="U2R41_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEM8888296.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="U2R41_000867"
     CDS             complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="U2R41_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEM8888297.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2400..2795)
                     /locus_tag="U2R41_000868"
     CDS             complement(2400..2795)
                     /locus_tag="U2R41_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEM8888298.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2792..4204)
                     /locus_tag="U2R41_000869"
     CDS             complement(2792..4204)
                     /locus_tag="U2R41_000869"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEM8888299.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="U2R41_000870"
     CDS             complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="U2R41_000870"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEM8888300.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4823..5062)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5059..6519)
                     /locus_tag="U2R41_000871"
     CDS             complement(5059..6519)
                     /locus_tag="U2R41_000871"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEM8888301.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6689..7846)
                     /locus_tag="U2R41_000872"
     CDS             complement(6689..7846)
                     /locus_tag="U2R41_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEM8888302.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7880..9250)
                     /gene="radA"
                     /locus_tag="U2R41_000873"
     CDS             complement(7880..9250)
                     /gene="radA"
                     /locus_tag="U2R41_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEM8888303.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9313..9840)
                     /locus_tag="U2R41_000874"
     CDS             complement(9313..9840)
                     /locus_tag="U2R41_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEM8888304.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10070..10876
                     /gene="proB"
                     /locus_tag="U2R41_000875"
     CDS             10070..10876
                     /gene="proB"
                     /locus_tag="U2R41_000875"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEM8888305.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10873..12120
                     /locus_tag="U2R41_000876"
     CDS             10873..12120
                     /locus_tag="U2R41_000876"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEM8888306.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12196..12540
                     /locus_tag="U2R41_000877"
     CDS             12196..12540
                     /locus_tag="U2R41_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888307.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12657..12983)
                     /locus_tag="U2R41_000878"
     CDS             complement(12657..12983)
                     /locus_tag="U2R41_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888308.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13356..14557)
                     /locus_tag="U2R41_000879"
                     /pseudo
     CDS             complement(13356..14557)
                     /locus_tag="U2R41_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14674..15189
                     /locus_tag="U2R41_000880"
     CDS             14674..15189
                     /locus_tag="U2R41_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8888309.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15388..15834)
                     /locus_tag="U2R41_000881"
     CDS             complement(15388..15834)
                     /locus_tag="U2R41_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM8888310.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15881..17359)
                     /gene="msr(C)"
                     /locus_tag="U2R41_000882"
     CDS             complement(15881..17359)
                     /gene="msr(C)"
                     /locus_tag="U2R41_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM8888311.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17713..18342)
                     /locus_tag="U2R41_000883"
     CDS             complement(17713..18342)
                     /locus_tag="U2R41_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8888312.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(18326..18790)
                     /locus_tag="U2R41_000884"
     CDS             complement(18326..18790)
                     /locus_tag="U2R41_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8888313.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19277..20545)
                     /locus_tag="U2R41_000885"
     CDS             complement(19277..20545)
                     /locus_tag="U2R41_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8888314.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20776..21513)
                     /locus_tag="U2R41_000886"
     CDS             complement(20776..21513)
                     /locus_tag="U2R41_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8888315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21580..22149)
                     /locus_tag="U2R41_000887"
     CDS             complement(21580..22149)
                     /locus_tag="U2R41_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM8888316.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22277..23149
                     /locus_tag="U2R41_000888"
     CDS             22277..23149
                     /locus_tag="U2R41_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8888317.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23491..24363
                     /locus_tag="U2R41_000889"
     CDS             23491..24363
                     /locus_tag="U2R41_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8888318.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24360..26318
                     /locus_tag="U2R41_000890"
     CDS             24360..26318
                     /locus_tag="U2R41_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8888319.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26569..27420)
                     /locus_tag="U2R41_000891"
     CDS             complement(26569..27420)
                     /locus_tag="U2R41_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8888320.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27543..28010
                     /locus_tag="U2R41_000892"
     CDS             27543..28010
                     /locus_tag="U2R41_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM8888321.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28273..28755)
                     /locus_tag="U2R41_000893"
     CDS             complement(28273..28755)
                     /locus_tag="U2R41_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEM8888322.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28855..29718)
                     /locus_tag="U2R41_000894"
     CDS             complement(28855..29718)
                     /locus_tag="U2R41_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8888323.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30316..31164
                     /locus_tag="U2R41_000895"
     CDS             30316..31164
                     /locus_tag="U2R41_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888324.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(31335..31676)
                     /locus_tag="U2R41_000896"
     CDS             complement(31335..31676)
                     /locus_tag="U2R41_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEM8888325.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31762..32469
                     /locus_tag="U2R41_000897"
     CDS             31762..32469
                     /locus_tag="U2R41_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM8888326.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32456..32779
                     /locus_tag="U2R41_000898"
     CDS             32456..32779
                     /locus_tag="U2R41_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM8888327.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32868..33905)
                     /locus_tag="U2R41_000899"
     CDS             complement(32868..33905)
                     /locus_tag="U2R41_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8888328.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(34352..35335)
                     /locus_tag="U2R41_000900"
     CDS             complement(34352..35335)
                     /locus_tag="U2R41_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEM8888329.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35751..36521)
                     /locus_tag="U2R41_000901"
     CDS             complement(35751..36521)
                     /locus_tag="U2R41_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888330.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36514..37449)
                     /locus_tag="U2R41_000902"
     CDS             complement(36514..37449)
                     /locus_tag="U2R41_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888331.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(38043..39692)
                     /locus_tag="U2R41_000903"
     CDS             complement(38043..39692)
                     /locus_tag="U2R41_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM8888332.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39887..40783)
                     /locus_tag="U2R41_000904"
     CDS             complement(39887..40783)
                     /locus_tag="U2R41_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8888333.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40776..41456)
                     /locus_tag="U2R41_000905"
     CDS             complement(40776..41456)
                     /locus_tag="U2R41_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888334.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41457..42239)
                     /locus_tag="U2R41_000906"
     CDS             complement(41457..42239)
                     /locus_tag="U2R41_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888335.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(42232..43149)
                     /locus_tag="U2R41_000907"
     CDS             complement(42232..43149)
                     /locus_tag="U2R41_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888336.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(43152..43436)
                     /locus_tag="U2R41_000908"
     CDS             complement(43152..43436)
                     /locus_tag="U2R41_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888337.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            44224..47451
                     /gene="fss3"
                     /locus_tag="U2R41_000909"
     CDS             44224..47451
                     /gene="fss3"
                     /locus_tag="U2R41_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM8888338.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            47564..47878
                     /locus_tag="U2R41_000910"
     CDS             47564..47878
                     /locus_tag="U2R41_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8888339.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            47891..48265
                     /locus_tag="U2R41_000911"
     CDS             47891..48265
                     /locus_tag="U2R41_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8888340.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            48275..48610
                     /locus_tag="U2R41_000912"
     CDS             48275..48610
                     /locus_tag="U2R41_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEM8888341.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            48693..>49819
                     /locus_tag="U2R41_000913"
     CDS             48693..>49819
                     /locus_tag="U2R41_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8888342.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        14667 a        10354 c         9163 g        15635 t
ORIGIN      
        1 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
       61 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
      121 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
      181 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
      241 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
      301 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
      361 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      421 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      481 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      541 tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa
      601 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      661 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt
      721 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      781 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      841 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      901 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      961 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
     1021 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
     1081 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
     1141 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
     1201 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
     1261 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1321 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1381 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1441 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1501 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1561 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1621 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1681 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1741 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1801 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1861 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1921 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1981 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     2041 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     2101 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     2161 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     2221 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     2281 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2341 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2401 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2461 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2521 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2581 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2641 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2701 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2761 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2821 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2881 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2941 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     3001 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     3061 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     3121 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     3181 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     3241 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3301 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3361 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3421 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3481 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3541 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3601 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3661 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3721 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3781 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3841 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg
     3901 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3961 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     4021 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     4081 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     4141 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     4201 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     4261 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4321 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4381 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4441 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4501 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4561 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4621 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4681 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4741 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4801 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4861 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4921 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4981 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     5041 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     5101 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     5161 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     5221 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     5281 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5341 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5401 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5461 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5521 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5581 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5641 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5701 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5761 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5821 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5881 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5941 cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     6001 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     6061 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     6121 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     6181 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     6241 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6301 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6361 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6421 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6481 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6541 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6601 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6661 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
     6721 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
     6781 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
     6841 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
     6901 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
     6961 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
     7021 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
     7081 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
     7141 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
     7201 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
     7261 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
     7321 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
     7381 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
     7441 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
     7501 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
     7561 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
     7621 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     7681 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     7741 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     7801 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     7861 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     7921 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     7981 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     8041 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     8101 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     8161 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     8221 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     8281 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     8341 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     8401 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     8461 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     8521 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     8581 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     8641 cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     8701 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     8761 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     8821 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     8881 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     8941 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     9001 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     9061 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     9121 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     9181 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     9241 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     9301 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     9361 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     9421 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     9481 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     9541 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     9601 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     9661 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     9721 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     9781 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     9841 cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt
     9901 tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt
     9961 tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac
    10021 aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga
    10081 aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa
    10141 tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa
    10201 tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt
    10261 acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca
    10321 agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca
    10381 gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac
    10441 attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc
    10501 agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat
    10561 ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc
    10621 agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga
    10681 aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag
    10741 taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg
    10801 aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa
    10861 ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa
    10921 caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc
    10981 atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc
    11041 gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat
    11101 gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat
    11161 gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg
    11221 accggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc
    11281 aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta
    11341 ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag
    11401 tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta
    11461 gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg
    11521 gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag
    11581 ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac
    11641 gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa
    11701 aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta
    11761 gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt
    11821 gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact
    11881 aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa
    11941 gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt
    12001 ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg
    12061 ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa
    12121 tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac
    12181 tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg
    12241 aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga
    12301 agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg
    12361 ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat
    12421 tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt
    12481 tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa
    12541 aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt
    12601 agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac
    12661 cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg
    12721 ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct
    12781 tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc
    12841 tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca
    12901 cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact
    12961 tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa atgaatttta
    13021 gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa
    13081 aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat
    13141 aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag
    13201 agttaaacga cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt
    13261 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
    13321 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
    13381 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
    13441 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
    13501 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
    13561 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
    13621 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
    13681 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
    13741 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
    13801 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
    13861 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
    13921 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
    13981 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
    14041 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
    14101 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
    14161 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
    14221 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
    14281 cctgtcatca atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca
    14341 ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt
    14401 ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga
    14461 tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt
    14521 gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat
    14581 acatgattta ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat
    14641 aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta
    14701 tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt
    14761 tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca gcagggttat
    14821 gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa
    14881 ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat
    14941 gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg
    15001 ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat
    15061 gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt
    15121 atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt
    15181 tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc
    15241 aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat
    15301 cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa
    15361 ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact
    15421 tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt
    15481 ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc
    15541 gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat
    15601 ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc
    15661 gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa
    15721 acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat
    15781 caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta
    15841 ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta
    15901 ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt
    15961 aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag
    16021 cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg
    16081 gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata
    16141 acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg
    16201 attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt
    16261 gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc
    16321 cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg
    16381 cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc
    16441 tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg
    16501 gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga
    16561 gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt
    16621 tagaggaaga caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt
    16681 ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt
    16741 tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat
    16801 aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg
    16861 atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat
    16921 aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt
    16981 ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt
    17041 cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta
    17101 attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg
    17161 tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca
    17221 aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac
    17281 gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat
    17341 ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga
    17401 tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc
    17461 tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt
    17521 cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat
    17581 ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga
    17641 cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta
    17701 actgaaaacc ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta
    17761 accttcttct accgcttttc ctattgataa aataataact ggaacataac ggtctttatc
    17821 caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata
    17881 gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac
    17941 tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg
    18001 ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc
    18061 gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt
    18121 ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc
    18181 gaccacaaag cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat
    18241 catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct
    18301 tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg
    18361 tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt
    18421 tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt
    18481 cacaatgctc gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt
    18541 tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag
    18601 taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt
    18661 cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa
    18721 agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt
    18781 ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa
    18841 tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat
    18901 agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc
    18961 acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag
    19021 tagaaaattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat
    19081 attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca
    19141 caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa
    19201 acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct
    19261 ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg
    19321 gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat
    19381 ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat
    19441 aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt
    19501 ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag
    19561 caatcccttc atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg
    19621 tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg
    19681 gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat
    19741 aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg
    19801 tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca
    19861 ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat
    19921 tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat
    19981 tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc
    20041 cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac
    20101 ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag
    20161 atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat
    20221 gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg
    20281 gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag
    20341 ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct
    20401 ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg
    20461 tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc
    20521 taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa
    20581 ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt
    20641 tctttttgat aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc
    20701 attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat
    20761 attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga
    20821 catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa
    20881 tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg
    20941 atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg
    21001 ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac
    21061 ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga
    21121 tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt
    21181 gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat
    21241 gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat
    21301 aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc
    21361 tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt
    21421 agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat
    21481 gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc
    21541 tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg
    21601 tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac
    21661 agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc
    21721 tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg
    21781 ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac
    21841 tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac
    21901 atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac
    21961 cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc
    22021 atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat
    22081 cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag
    22141 gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta
    22201 atacttataa aatatactta actattcctt ttaactatac ttagtggtat gcttttttaa
    22261 aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga
    22321 aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca
    22381 actgaaagat ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat
    22441 acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt
    22501 agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg
    22561 cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc
    22621 gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact
    22681 ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca
    22741 gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt
    22801 agcaaagcaa accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg
    22861 gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc
    22921 gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt
    22981 tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc
    23041 actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc
    23101 tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt
    23161 ttccttcgct ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat
    23221 aacataagtt tcttaattta tttcttcctt ttatttctga taattccagt aaattactat
    23281 aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa
    23341 taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt
    23401 ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt
    23461 gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat
    23521 gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt
    23581 gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa
    23641 ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt
    23701 gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat
    23761 gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc
    23821 gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt
    23881 gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa
    23941 gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc
    24001 agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa
    24061 acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga
    24121 ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa
    24181 atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg
    24241 accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc
    24301 gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat
    24361 tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg
    24421 ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg
    24481 ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg
    24541 ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg
    24601 tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact ttattgaata
    24661 tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta
    24721 ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg
    24781 ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc
    24841 tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg
    24901 ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt
    24961 tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag
    25021 gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt
    25081 atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt
    25141 acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt
    25201 ttgcttcatt ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt
    25261 ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct
    25321 tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga
    25381 aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga
    25441 tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg
    25501 aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg
    25561 cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata
    25621 actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa
    25681 tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg
    25741 taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag
    25801 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg
    25861 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
    25921 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
    25981 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
    26041 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
    26101 aaggcgaacc attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga
    26161 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
    26221 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
    26281 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
    26341 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
    26401 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa
    26461 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt
    26521 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    26581 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    26641 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    26701 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    26761 tgcttcaata atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc
    26821 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    26881 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    26941 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    27001 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    27061 gaattggtga tctggcgcta atatactgat cgagtgatcg gcatctgcaa aacttgtata
    27121 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    27181 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    27241 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    27301 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    27361 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    27421 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    27481 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    27541 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    27601 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    27661 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    27721 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    27781 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    27841 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    27901 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    27961 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    28021 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    28081 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    28141 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    28201 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    28261 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    28321 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    28381 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    28441 ttctgctaaa gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt
    28501 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    28561 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    28621 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    28681 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    28741 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    28801 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    28861 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    28921 tgtaaaagag atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc
    28981 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    29041 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    29101 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa
    29161 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    29221 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    29281 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    29341 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    29401 ttgtatgccg tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    29461 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    29521 atctagaaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    29581 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    29641 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    29701 gttcagatct ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa
    29761 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    29821 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    29881 tatgttttac ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg
    29941 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    30001 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    30061 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    30121 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    30181 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    30241 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    30301 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    30361 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    30421 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    30481 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    30541 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    30601 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    30661 atagaacatc cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    30721 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    30781 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    30841 tcgcttattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    30901 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    30961 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    31021 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct
    31081 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    31141 gataaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
    31201 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
    31261 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
    31321 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg
    31381 cattgttgat agattttatg atacttttct acattttgta agagaggttc cgctgcatct
    31441 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt
    31501 gcagcaatca ttgttgcgcc taatgcaggt ccttcctcca cacttaaaca atagaccgtt
    31561 gcatcgaaaa tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc
    31621 gaaacaatac gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac
    31681 aaatgctcat taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt
    31741 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
    31801 acgttaccta ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa
    31861 gctgccggat ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct
    31921 gcccaattta tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc
    31981 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
    32041 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
    32101 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
    32161 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
    32221 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
    32281 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
    32341 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac
    32401 ttagtgattg tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
    32461 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
    32521 ggtcctacca tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt
    32581 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
    32641 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
    32701 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc
    32761 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
    32821 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
    32881 ggaccactaa caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt
    32941 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
    33001 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
    33061 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
    33121 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
    33181 acaaggtgct tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
    33241 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
    33301 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
    33361 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg
    33421 atcactaaga tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca
    33481 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa
    33541 agattttttg gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt
    33601 cctgctcttg ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
    33661 tcgtcaccta cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt
    33721 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
    33781 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
    33841 tttggatcat cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct
    33901 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa
    33961 tatatatagt atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa
    34021 agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac
    34081 ctcttataaa cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac
    34141 atgagaagct gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa
    34201 cctctgatat aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa
    34261 atatgaagag ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac
    34321 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
    34381 ctttttcaaa ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
    34441 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
    34501 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
    34561 gaagtagtcc gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag
    34621 gattcttgaa aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt
    34681 cttctcctgt ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga
    34741 tttggtcacg taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca
    34801 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
    34861 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
    34921 gcaaaccgcc agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca
    34981 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
    35041 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
    35101 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta
    35161 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
    35221 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
    35281 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
    35341 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
    35401 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
    35461 aaaaaagact gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa
    35521 agcttgcatt tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt
    35581 ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt
    35641 caatcagctg accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc
    35701 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc
    35761 aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac
    35821 acaagtacga tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg
    35881 aaaccggaga tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt
    35941 aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta
    36001 ggcacgatcg atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat
    36061 gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga
    36121 agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc
    36181 acaccaccaa tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt
    36241 tcttcgatat ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa
    36301 ctagtgatag cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca
    36361 cccatgtcac cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc
    36421 gtgatcactg gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg
    36481 atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt
    36541 attttcttct gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga
    36601 aagaactttg attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg
    36661 ttcaacttcg acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac
    36721 aggtttggta tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag
    36781 aagctctttc atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg
    36841 acaaagcatt tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc
    36901 gttcaattct cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt
    36961 tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct
    37021 tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga
    37081 ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc
    37141 tgcttgattc acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg
    37201 aaccaatcca atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac
    37261 attcactttt ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt
    37321 cccagctcca tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat
    37381 tccccgcaat gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc
    37441 atttgccatt tatttcctat ctccttatag acatgattct taattgtatc atggaataca
    37501 gcagatgaaa aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg
    37561 tgttttctag ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt
    37621 aaaatcataa aagtaccgac agcaactact acgatgacat atagaagatc cttataactg
    37681 aggttcttgt cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt
    37741 tcttttattt taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc
    37801 tgtttgtttt acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt
    37861 ttcgataaaa gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa
    37921 tcttccaaga tcatctggac agcagactcg ttcccattga attctgccac ttttttggca
    37981 tcaatgtact tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa
    38041 agttaatcaa acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc
    38101 tgttcaacta tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc
    38161 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
    38221 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
    38281 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
    38341 gcaatccgtt cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt
    38401 tctttggaag ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa
    38461 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
    38521 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
    38581 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
    38641 tgccagttga ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct
    38701 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc
    38761 aaaacggctg ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt
    38821 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
    38881 atgttgacat attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa
    38941 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt
    39001 gtcgtcattt ttgctaactg attggcggct gaaatcgttg aaaccaaata actcatgcta
    39061 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag
    39121 ccaccgattg ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc
    39181 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
    39241 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
    39301 tggattcttt ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga
    39361 taatcttctc caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta
    39421 gaataggtac tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga
    39481 tcgtcgattt cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa
    39541 ctttcttctt tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt
    39601 tcctttactt tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata
    39661 ataattaaaa cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt
    39721 tttagtatag cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac
    39781 ctaattcgta tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa
    39841 acgagataca attttttgtc attcacttca ggtaaattag agccaattaa accctccgca
    39901 acttgacgga gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc
    39961 cttccaccaa tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc
    40021 tggtattttc ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt
    40081 tgataggatt ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg
    40141 cttgtccatc tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca
    40201 tgagcggatc tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat
    40261 ttttctcaga aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag
    40321 aaagcgtatg gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact
    40381 gatccgtcat actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc
    40441 gtttctccgt atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag
    40501 gagttctgag atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt
    40561 gaaatgcttg tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt
    40621 ccattagatt cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag
    40681 aaagagtggc gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac
    40741 ttcccataaa gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc
    40801 cacaccgtat gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata
    40861 tgcatattca ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga
    40921 tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct
    40981 cttcctgtca aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt
    41041 aagatttcct tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga
    41101 cgaagttgga tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag
    41161 tcatccgctc ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga
    41221 acgatgatcg gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc
    41281 cctggaagca tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt
    41341 ccttccgtac ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt
    41401 aaagaatgaa cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac
    41461 ttaatgtcag atttttggaa aataaacatt cctagagctg atgataccac gattgttact
    41521 agactgacca gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta
    41581 tacatgagcc acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg
    41641 aaaacaccgg gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca
    41701 ctggtacttc tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc
    41761 agaagcaata caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact
    41821 tcaccaaatc cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg
    41881 ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc
    41941 cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag
    42001 cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc
    42061 cctgttgcac cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta
    42121 tgcatatacc attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag
    42181 acccacattt tacggctctt ccatactttt attttttcag cagttaatag attaaacatg
    42241 tggattgcct ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat
    42301 accatccaca ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat
    42361 tccttcaaaa agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga
    42421 gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga
    42481 ttcttgtgcc aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc
    42541 gtagtgagtg gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat
    42601 gccccgttct tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc
    42661 catcccattc gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc
    42721 aatagctaaa cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt
    42781 ttccaatcgc atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc
    42841 tgcttggata cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat
    42901 cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga
    42961 accttcatca atcgagatca aacccataat agttctcata aaggtagact tacccgctcc
    43021 attctcccca atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa
    43081 tgctttggca gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc
    43141 aaaatccata cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag
    43201 aaagcagcca gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc
    43261 agactgtctc gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct
    43321 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt
    43381 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
    43441 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
    43501 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
    43561 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
    43621 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
    43681 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
    43741 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
    43801 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
    43861 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
    43921 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
    43981 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
    44041 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
    44101 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
    44161 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
    44221 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
    44281 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
    44341 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
    44401 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
    44461 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
    44521 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
    44581 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
    44641 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
    44701 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
    44761 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
    44821 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
    44881 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
    44941 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
    45001 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
    45061 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
    45121 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
    45181 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
    45241 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
    45301 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
    45361 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
    45421 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
    45481 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
    45541 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
    45601 gccaatcaaa ccgtagccct tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
    45661 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
    45721 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
    45781 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
    45841 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
    45901 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
    45961 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
    46021 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
    46081 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
    46141 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
    46201 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
    46261 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
    46321 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
    46381 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
    46441 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
    46501 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
    46561 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
    46621 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
    46681 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
    46741 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
    46801 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
    46861 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
    46921 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
    46981 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
    47041 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
    47101 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
    47161 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
    47221 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
    47281 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
    47341 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
    47401 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
    47461 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
    47521 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
    47581 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
    47641 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
    47701 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
    47761 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
    47821 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
    47881 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
    47941 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
    48001 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
    48061 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
    48121 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
    48181 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
    48241 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
    48301 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
    48361 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
    48421 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
    48481 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
    48541 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
    48601 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
    48661 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
    48721 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
    48781 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
    48841 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
    48901 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
    48961 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
    49021 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
    49081 acttccccaa aatctattat cgaatgaaag atggattact gtctattcgc gttcaaattt
    49141 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
    49201 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
    49261 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
    49321 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
    49381 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
    49441 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
    49501 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
    49561 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
    49621 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    49681 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    49741 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    49801 gagacggaat ccgcgatca
//
LOCUS       DASHWH010000016        49641 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.016, whole genome shotgun sequence.
ACCESSION   DASHWH010000016 DASHWH010000000
VERSION     DASHWH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.016"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            171..1229
                     /locus_tag="U2R41_000914"
     CDS             171..1229
                     /locus_tag="U2R41_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEM8888343.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMIGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1233..2267
                     /locus_tag="U2R41_000915"
     CDS             1233..2267
                     /locus_tag="U2R41_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEM8888344.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2369..3085)
                     /locus_tag="U2R41_000916"
     CDS             complement(2369..3085)
                     /locus_tag="U2R41_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8888345.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3054..3839)
                     /locus_tag="U2R41_000917"
     CDS             complement(3054..3839)
                     /locus_tag="U2R41_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEM8888346.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3830..4579)
                     /locus_tag="U2R41_000918"
     CDS             complement(3830..4579)
                     /locus_tag="U2R41_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM8888347.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4877..5401
                     /locus_tag="U2R41_000919"
     CDS             4877..5401
                     /locus_tag="U2R41_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEM8888348.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5417..6916
                     /locus_tag="U2R41_000920"
     CDS             5417..6916
                     /locus_tag="U2R41_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEM8888349.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7004..7606)
                     /locus_tag="U2R41_000921"
     CDS             complement(7004..7606)
                     /locus_tag="U2R41_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8888350.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7710..8666
                     /locus_tag="U2R41_000922"
     CDS             7710..8666
                     /locus_tag="U2R41_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEM8888351.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8971..9927)
                     /locus_tag="U2R41_000923"
     CDS             complement(8971..9927)
                     /locus_tag="U2R41_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8888352.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10101..10661
                     /locus_tag="U2R41_000924"
     CDS             10101..10661
                     /locus_tag="U2R41_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888353.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10654..11478
                     /locus_tag="U2R41_000925"
     CDS             10654..11478
                     /locus_tag="U2R41_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEM8888354.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11488..12105
                     /locus_tag="U2R41_000926"
     CDS             11488..12105
                     /locus_tag="U2R41_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8888355.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12123..12806
                     /locus_tag="U2R41_000927"
     CDS             12123..12806
                     /locus_tag="U2R41_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM8888356.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13489..14772
                     /locus_tag="U2R41_000928"
     CDS             13489..14772
                     /locus_tag="U2R41_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8888357.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELVIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14788..15675
                     /locus_tag="U2R41_000929"
     CDS             14788..15675
                     /locus_tag="U2R41_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8888358.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15692..16528
                     /locus_tag="U2R41_000930"
     CDS             15692..16528
                     /locus_tag="U2R41_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8888359.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16531..17154
                     /locus_tag="U2R41_000931"
     CDS             16531..17154
                     /locus_tag="U2R41_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888360.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17151..18443
                     /locus_tag="U2R41_000932"
     CDS             17151..18443
                     /locus_tag="U2R41_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8888361.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18802..18899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19015..19596
                     /locus_tag="U2R41_000933"
     CDS             19015..19596
                     /locus_tag="U2R41_000933"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEM8888362.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19599..20906
                     /locus_tag="U2R41_000934"
     CDS             19599..20906
                     /locus_tag="U2R41_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEM8888363.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21322..21810
                     /gene="purE"
                     /locus_tag="U2R41_000935"
     CDS             21322..21810
                     /gene="purE"
                     /locus_tag="U2R41_000935"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEM8888364.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21803..22927
                     /gene="purK"
                     /locus_tag="U2R41_000936"
     CDS             21803..22927
                     /gene="purK"
                     /locus_tag="U2R41_000936"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEM8888365.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22940..24235
                     /gene="purB"
                     /locus_tag="U2R41_000937"
     CDS             22940..24235
                     /gene="purB"
                     /locus_tag="U2R41_000937"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEM8888366.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24415..24792)
                     /locus_tag="U2R41_000938"
     CDS             complement(24415..24792)
                     /locus_tag="U2R41_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888367.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25048..25776
                     /locus_tag="U2R41_000939"
     CDS             25048..25776
                     /locus_tag="U2R41_000939"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEM8888368.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25776..26030
                     /gene="purS"
                     /locus_tag="U2R41_000940"
     CDS             25776..26030
                     /gene="purS"
                     /locus_tag="U2R41_000940"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEM8888369.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26032..26703
                     /gene="purQ"
                     /locus_tag="U2R41_000941"
     CDS             26032..26703
                     /gene="purQ"
                     /locus_tag="U2R41_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEM8888370.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26704..28926
                     /gene="purL"
                     /locus_tag="U2R41_000942"
     CDS             26704..28926
                     /gene="purL"
                     /locus_tag="U2R41_000942"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEM8888371.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28911..30350
                     /locus_tag="U2R41_000943"
     CDS             28911..30350
                     /locus_tag="U2R41_000943"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEM8888372.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30373..31425
                     /locus_tag="U2R41_000944"
     CDS             30373..31425
                     /locus_tag="U2R41_000944"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEM8888373.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31422..32000
                     /gene="purN"
                     /locus_tag="U2R41_000945"
     CDS             31422..32000
                     /gene="purN"
                     /locus_tag="U2R41_000945"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEM8888374.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31997..33532
                     /gene="purH"
                     /locus_tag="U2R41_000946"
     CDS             31997..33532
                     /gene="purH"
                     /locus_tag="U2R41_000946"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEM8888375.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33548..34822
                     /gene="purD"
                     /locus_tag="U2R41_000947"
     CDS             33548..34822
                     /gene="purD"
                     /locus_tag="U2R41_000947"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEM8888376.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34944..35858
                     /locus_tag="U2R41_000948"
     CDS             34944..35858
                     /locus_tag="U2R41_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8888377.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35991..37556)
                     /locus_tag="U2R41_000949"
     CDS             complement(35991..37556)
                     /locus_tag="U2R41_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8888378.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37549..39000)
                     /locus_tag="U2R41_000950"
     CDS             complement(37549..39000)
                     /locus_tag="U2R41_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8888379.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39014..40030)
                     /locus_tag="U2R41_000951"
     CDS             complement(39014..40030)
                     /locus_tag="U2R41_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888380.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40250..41338
                     /locus_tag="U2R41_000952"
     CDS             40250..41338
                     /locus_tag="U2R41_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEM8888381.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41392..42918
                     /locus_tag="U2R41_000953"
     CDS             41392..42918
                     /locus_tag="U2R41_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEM8888382.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42920..44125
                     /locus_tag="U2R41_000954"
     CDS             42920..44125
                     /locus_tag="U2R41_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8888383.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44382..45359
                     /gene="guaC"
                     /locus_tag="U2R41_000955"
     CDS             44382..45359
                     /gene="guaC"
                     /locus_tag="U2R41_000955"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEM8888384.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45623..47047
                     /locus_tag="U2R41_000956"
     CDS             45623..47047
                     /locus_tag="U2R41_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM8888385.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47508..48617
                     /gene="pdhA"
                     /locus_tag="U2R41_000957"
     CDS             47508..48617
                     /gene="pdhA"
                     /locus_tag="U2R41_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEM8888386.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48620..49597
                     /locus_tag="U2R41_000958"
     CDS             48620..49597
                     /locus_tag="U2R41_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEM8888387.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        15833 a         8541 c        10917 g        14350 t
ORIGIN      
        1 ttttttttgt gttctagtaa aaatcacttt acataagttt caaaagtttc tttaatttcc
       61 tattgttcaa taagtaacaa tgtaaagata aaattaaaaa tgttaatacg tattagcaaa
      121 ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag atgaacttat
      181 ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc acttcaacag
      241 tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct gaatctgttg
      301 gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca tttcgagctt
      361 tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg atttggggat
      421 tagttattta tattattctt attctagttt caaaaccttt tttcttaagc tataaagggg
      481 ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt ttcggaacgc
      541 ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt ttcaatattg
      601 gctaccgaat ctttttgtat tcttatggat acataaaaat gattggcttg aagatggaag
      661 ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt gccggccttt
      721 tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca gaaggagaga
      781 taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt cagccgatgg
      841 attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca acattaggtg
      901 aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt ctagtcaaag
      961 tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca acgaatattc
     1021 taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca ccagctgcaa
     1081 cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc tccaatgcat
     1141 ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt gtattacaaa
     1201 tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg agaatcgcat
     1261 gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt taccattatg
     1321 atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca aaggaccatg
     1381 atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca aaaatgcagg
     1441 aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc gatctacaag
     1501 cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca aatgcgatag
     1561 cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat acgacgatcc
     1621 gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa atcagaaatt
     1681 gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt ttgtttaagg
     1741 gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct aaaaaagtat
     1801 tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt attcatgtac
     1861 ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct caaatgaaaa
     1921 aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa gctattttaa
     1981 gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca aatgaaacag
     2041 aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct atccagcaat
     2101 tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat acagatgagg
     2161 cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata gaaactggaa
     2221 aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa aacatggata
     2281 ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct gtatacttgg
     2341 actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt ttccgcccat
     2401 tctttgtcaa ttcataacag gttagcggcc ggccgatttc caaatatttg attctttcgt
     2461 tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata cgtgacagtg
     2521 agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga ataaatgcac
     2581 ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat gatggtattt
     2641 cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg gctttttcag
     2701 tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga aaacgttgca
     2761 aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc tttatggttt
     2821 cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc tgtctaagcc
     2881 aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc agcagatctg
     2941 cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt tcgattacgt
     3001 tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt ggatcatctt
     3061 cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc tagatagacg
     3121 attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg tttgacgatt
     3181 gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa acctagcgta
     3241 aatatttttt ctgagatttc ttctgatatc ccgacaccgt catcgcagac ggtcacgatg
     3301 aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt tctttcatgt
     3361 ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg caaaagtgaa
     3421 tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa cagcatttca
     3481 acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat tgcaggtact
     3541 ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag ctgctggata
     3601 aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac atgaagttta
     3661 ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt ccccccaagc
     3721 tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc tcctaaaaaa
     3781 cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt taaatacatg
     3841 gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac gtcatataga
     3901 ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg tagaaaatgt
     3961 tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct aagattcctt
     4021 catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt tcttgtggtt
     4081 caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct atgatgccta
     4141 cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg acttgattgt
     4201 caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga tcataataaa
     4261 tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag aaaaattctt
     4321 gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg atgtaacttg
     4381 ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa tctaaattcg
     4441 atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca ccagtctgca
     4501 gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag attttatctg
     4561 acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca gaaacaatca
     4621 aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc tttaatggtt
     4681 ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa aattttaaca
     4741 tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata aaaaacacca
     4801 ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag acgtagaaat
     4861 ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt ggtatacatt
     4921 gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat tcgattttga
     4981 tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct tatttggttt
     5041 tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta gtggaggcat
     5101 atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga aacatgccga
     5161 atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg tgatcacacc
     5221 gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt ccattagtcg
     5281 tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga aagtattgat
     5341 cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga taaaagctta
     5401 aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa tcgcagcttt
     5461 gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa aaccggatga
     5521 agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg atgaaagcaa
     5581 taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt ttcaacgttc
     5641 gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag tgtatacgga
     5701 acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt catctgtaga
     5761 agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac tggaaaatga
     5821 agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga ctgtagaaga
     5881 aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt ctgtagacct
     5941 tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag acaaaaacgg
     6001 ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg cagatatagc
     6061 agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa aagaatcaca
     6121 agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa aagaactgaa
     6181 attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc aagcctacaa
     6241 tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga ttaaaatcgt
     6301 agaacgtcaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg aaaagcaata
     6361 tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac aagaagcaca
     6421 agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg tggaagcact
     6481 ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg aagcagcact
     6541 agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct tcaaggaata
     6601 tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga tgcgcgaagc
     6661 agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa gcagttcagg
     6721 agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt cttctactca
     6781 agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga atttgtcgac
     6841 acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag aacaaaactc
     6901 gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag gaagactttt
     6961 taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc tcgctatttt
     7021 tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc aatcgttctt
     7081 tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt tcaggaggac
     7141 gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct agtacacgtt
     7201 ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc aacaacgtag
     7261 ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg atcacaataa
     7321 taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa tagcttttga
     7381 caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc ctagactttc
     7441 caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat aatgcagcaa
     7501 tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa acagctaagg
     7561 cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag attctccttt
     7621 ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc attattcaag
     7681 tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca gtcaaaacag
     7741 tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg tattggccag
     7801 ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca ttgcgtacgg
     7861 gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc agtttttttg
     7921 ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc ccgcttgcct
     7981 ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact cattatttag
     8041 tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg gcgattggat
     8101 tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag ctgaaggaaa
     8161 accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca gaatttcttt
     8221 tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca ttcattcgcg
     8281 aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag ccgctatact
     8341 acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac atgctggaaa
     8401 atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt ttattgattt
     8461 atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca cgtattgaag
     8521 aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa ttcgaggatc
     8581 gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc aaggcagaat
     8641 tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc aggtcaaaaa
     8701 gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg gtcacaagtc
     8761 acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcggagctg
     8821 aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc tgcggtaata
     8881 agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat
     8941 gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa cttgcatttg
     9001 atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca agtcaccttc
     9061 aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat tcccgctttc
     9121 gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac gcgggtaatc
     9181 agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca tttttgcctg
     9241 aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag tgacggattg
     9301 ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag gttgagagct
     9361 caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag ctcctccagc
     9421 taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc cgaaaggtgc
     9481 ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta actgctgata
     9541 aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt cttctgctaa
     9601 gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac atagaacgtg
     9661 tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg gtacagcaat
     9721 gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt aagcttttgg
     9781 aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag caactgctgt
     9841 tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg taccaagacc
     9901 aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg gattgagttt
     9961 cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg acttgaaaca
    10021 ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt aagtagaata
    10081 gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg agtgtattat
    10141 tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc agtccagaac
    10201 aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg cagaaagtaa
    10261 aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt caaacgatta
    10321 tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa acacgcatcg
    10381 cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg aaaaataatc
    10441 agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa tggggctatg
    10501 atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa ggagaaaaaa
    10561 tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat gaggcgaatg
    10621 gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg atttggagca
    10681 gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga ttcgcatgtt
    10741 caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag taaagaaaag
    10801 aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg tacgtccaaa
    10861 caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc agatcacaaa
    10921 ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg atacttgatc
    10981 gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc agctggattt
    11041 ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt tttcgaacaa
    11101 aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac aaagattact
    11161 aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc agtcgatgtc
    11221 gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag ttacggagat
    11281 cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat cgttcgttat
    11341 ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt acgttatgtc
    11401 ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga atatctgaat
    11461 gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa gcaaaagcgc
    11521 aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt agcctttgtt
    11581 ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa tgaagcgcaa
    11641 tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca agatggttat
    11701 ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg gggaaaaagt
    11761 accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga tcagaagaaa
    11821 gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca aggagacttc
    11881 aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt cgtccaagga
    11941 gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta tcaggaagcc
    12001 gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca aaaaatcatc
    12061 caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag gagaagtggc
    12121 tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat atcgtaagag
    12181 taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg atccgttcga
    12241 tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca gtaattgctg
    12301 tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca gctgacattc
    12361 cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc tacatgagca
    12421 tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg cctttagaga
    12481 cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta acgtcgaaat
    12541 atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc agtgcaggta
    12601 aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt cctatcattg
    12661 cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga gcaaatgcca
    12721 tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa aaatatcggg
    12781 aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga agagcagata
    12841 gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa aaaaaagaga
    12901 aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt ttgttattat
    12961 tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga atgaaaaacg
    13021 aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg gggcttgaaa
    13081 agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc gacgtgtata
    13141 ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg aagagcgcac
    13201 acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct gatatccttt
    13261 tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc gatgaaagaa
    13321 gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag gcagaacgat
    13381 aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata tgttctgtat
    13441 aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat gaagaaaaga
    13501 atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg tgggcaagaa
    13561 gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg acacgatgca
    13621 acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat cgattatgaa
    13681 ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc agatgatatc
    13741 tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat tgaaccatta
    13801 gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc ctatttagca
    13861 acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc gtttgcgatt
    13921 gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc aaattggaca
    13981 tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattagtaat ctatgctatt
    14041 tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt tgagaaagga
    14101 ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa tccagactat
    14161 ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca tccagaacca
    14221 ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga tagagaagcg
    14281 gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc tcctgaagat
    14341 atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc tggtatccct
    14401 ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga agaagctgct
    14461 aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa aggagaacgt
    14521 ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga tgcaactttg
    14581 caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga agtgaatgtc
    14641 tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt tgaaaaagtc
    14701 atttatgaag aagtaacagc agaagaagca gcagagagtc tttatgagtt tgcgtcaaac
    14761 attctaggat agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg gttcttggat
    14821 caagacaatg tggtcggtta cgtatttgct tctccattca tcctaggatt tctattgata
    14881 acagtgatac cgatgatctt gtcactttat tattcgttca ccgattatcg tttgggaaat
    14941 ccagtctcat ggatcggctt ggataattat ataaagatgc tgagtgatgc ccgttttttg
    15001 aactccatcc gagtcacttt tctctatgta tttgcatctg tacctgtgaa actgatcttt
    15061 ggtctattga ttgcttatct tttaacgagg aatatcaaag gaacgacttt ctttcgttct
    15121 ttgtattatg tgcctagttt gatcggtggc agtatttcta tcgctctagt ttggcgggag
    15181 ttgttttcta aaaacgggtt agtgaatatt ttcctggaac gattaggcgc acctgcgctt
    15241 tcctggttta gcgatcaaaa actggtcatg atccctcttg ttttgatgtc tgcttggcaa
    15301 ttcggttcgg caatgatcat ctttgctgca ggtatcaaag agatcccagg ctcgtattat
    15361 gaagcggcag agatcgatgg agcgaacaaa tggcagacgt tttacaagat cacgttacct
    15421 cttctttcgc cgatcatttt gtataacttg gttatgcaga caatcgcggc gtttatgact
    15481 tttacgcagg catttatcat tacccaaggt ggtccgaatg atgccacgaa cttttatgct
    15541 ctttatgtgt ataaccaagc tttttctttt aattctatgg ggtatgctag tgctatttcg
    15601 tggatcatgc tgttgattat gggagctgtc acctttctga ttttcaaatc atcaaaaaaa
    15661 tgggtcgttg tgtgaatgag aggaggaaga aatgaataat acagtaacga aatggtttca
    15721 atacggtttt attttgctat taggtctagt catgatttat ccggtcttat ggatgatcgc
    15781 aggttctttt aaaacggatc aagagattat gagtggttcg ctgaatttga ttccagagac
    15841 ttttcaatgg gaaaattacg caaaaggatg ggcggggttt gctggaattt cattttttac
    15901 tttctttagg aattcgtttg tcatctctgc agtttcgacg atagggacag ttctgagctc
    15961 gacttgtatt gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat tctggttcgt
    16021 cgtcatgtta gtaacgatga tgattcctgc acaagtgatt ttgattccgc agtttattat
    16081 ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg attttaccac atttttttgg
    16141 acaagcattt ttcatttacc agatcatgca attcatggtc agcatcccca aagagttaga
    16201 tgaatcagcg attattgacg gatgcagcaa gtatagtgtc tttaccaaaa ttatttttcc
    16261 tttgctgaaa ccttcgatca tcacgacgat catcatccag ttttattgga aatgggacga
    16321 cttcatggga ccattgattt atttaaacaa gccgagatct tacacggtat cgattgccat
    16381 caaattattt gcagacgctg gatctagtac gacttacgcg agtatgtttg cgatgtctac
    16441 gttgtcgctg ctaccggttt ttctgatttt cttattcttc aataaatatc ttgtccaggg
    16501 gatcagtaca agcggtctga agggctgaat atgaaaaagc caatagaaga gatgtttctt
    16561 tacaagctca actactatgt ctgtcggatt tttttaacca gtcttatcag taatgctctt
    16621 ttcgctttag ttctaggatg tttgttcact ttaggattag tgctgtctgc tcgtgttgtc
    16681 tatctgattt attttttgat gatgtatcct tcttattatc tatctgtcca ttacttggca
    16741 gaaaaaaagg tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat cattacgttg
    16801 tttcagctgt ttgtcattat ttttgcagtt gattggtatt tattagtggg aaagattgga
    16861 gcttggtatc tgattccttt tctattgtgt ctgttgcttt ttgtatccca tagttttttt
    16921 agtacgctaa ttgatatctg caaagaagat aaagcagata gtcttgttta tttgatcaaa
    16981 aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt atgccttttg
    17041 tatggactgt tctggtgtat acagcatgat tttttactca ctgcattact gtatccaggg
    17101 ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa atgaagaaaa
    17161 aatatgtaca agtcggactt ggtgggagag cgcgtttttt ttatgaggca attgcctcta
    17221 cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca aggatggatt
    17281 atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa agtaaagaca tgtatgccat
    17341 cacaatttga gcaactactg gatgacgtac ggccggctga agtcattatt acgtcagtag
    17401 atcgaacgca tcatgattat atcattcgtg caatgaaaaa aggatatgat gttatttgcg
    17461 aaaaacctct gactatcgat gaggaaaaag ctcaagaaat aataacggtc caaaaacaga
    17521 cgggaagaag cctgcgagtc acttttaatt atcgttatgc accgtatcac acgaagataa
    17581 aggaactgat agaaagtgat gtgattggtg atgtgttttc tatccacttc gaatggctgt
    17641 taaatacgca acacggggca gattatttca gaagatggca tcgaaataag cacaatagcg
    17701 gcgggcttct tgttcataaa tctacccatc attttgatct agtgaatttt tggttgaaca
    17761 gccgtcccca aaaggtattt gcattcggtg gattaaactt ttatgggatg cataatgcgg
    17821 agaaacgcgg agtagaaata ttttacagtc gatgctatgg aagcgaacta gctaaagctg
    17881 atccatttgc tatagacctg tcagccaatg aggaattgcg agaaatgtat ttagaagcag
    17941 aaaaagacag tggatatatt cgtgaccgca gtgtattttc cgatgatatc tctattgaag
    18001 atacactagg cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat tcgctaaacg
    18061 cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga cggatcgaaa
    18121 tgaaagtgat cgaaaagtct tacatcaatg caggtggaga taaaaaggat gaaggagcag
    18181 ccgttcttca gtccatccat gtctatccga tgttcggtga gccatatgag gtaatcgtcg
    18241 aacaagaagc gggcggacat ggcggaggag atccgcgttt attagatgat ctatttggtg
    18301 aagaaaaaga agaagatccg tggaatcggg cagcaactca cgtagatggt attttgtcga
    18361 ttttgacagg aatcgcagca aatcattcta ttgcttcagg aaaagcagta gtgattgacg
    18421 agcttgtcag cttaaaagaa taagaataga tttacggaaa ttacttcccg tagtttataa
    18481 aatcttgcct tattgccgat aggctgtctt ttgaacacta tttattcgga aaaaggggct
    18541 atgacatgct ttttgtcatg ccttcttttt atgtgggctg cttttttaga taggagaaaa
    18601 taaaaataac tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc attagatttt
    18661 acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac tgttttcaat
    18721 atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc
    18781 aagaaaatga atttacacta ttattagttt tacataccta cttatatatc gtcataatat
    18841 ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga ttggctattt
    18901 cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa catagtaggt
    18961 ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa taaagtgaaa
    19021 gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt attaaaagtc
    19081 gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg aaaacggttt
    19141 gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc ttctgggatc
    19201 gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc tagaaaagca
    19261 aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt tacgaaacaa
    19321 gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa agtattgatt
    19381 atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa
    19441 gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca agatggacgt
    19501 cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc ttccttgacg
    19561 aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg
    19621 gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg
    19681 tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact tatttgattt
    19741 caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt aaccgatttt
    19801 tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct ccactgatct
    19861 tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct tctgggatct
    19921 ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct cctatagtaa
    19981 caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc gagaaaatgg
    20041 gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta cttgcatttg
    20101 tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg
    20161 cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga atgattgatc
    20221 tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat tttggtaaac
    20281 caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc agcatggtag
    20341 aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt ggagaagaag
    20401 aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt attttcaata
    20461 cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa
    20521 caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta ttccctaaaa
    20581 tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg ttagtcatgt
    20641 tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc agcaatgata
    20701 aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat gttacgccga
    20761 cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc atcgtaatga
    20821 gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa
    20881 tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt
    20941 gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc gtttgtttga
    21001 gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg agcctgccgt
    21061 tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg ttgaatattg
    21121 ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag gaatgtgacc
    21181 taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa aatgtttttc
    21241 tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta
    21301 gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca
    21361 gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa
    21421 aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct
    21481 cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt
    21541 atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta
    21601 aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact
    21661 acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc
    21721 atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact
    21781 gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat
    21841 cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa
    21901 agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat
    21961 tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat
    22021 tacctatgaa ttcgaaaacg tcagtataga ggcgttaaat gcgattttac ccatgtcatt
    22081 tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt
    22141 tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat
    22201 ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta
    22261 tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct
    22321 tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat
    22381 catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca
    22441 ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga
    22501 ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg
    22561 ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc
    22621 tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat
    22681 tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat
    22741 ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga
    22801 ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat
    22861 cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg
    22921 ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg
    22981 gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg
    23041 ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt
    23101 tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac
    23161 acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag
    23221 tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg
    23281 ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac
    23341 agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact
    23401 tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg
    23461 agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat
    23521 tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatttcta ctcaagttct
    23581 ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga
    23641 acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt
    23701 ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc
    23761 agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa
    23821 tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc
    23881 tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct
    23941 aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag
    24001 ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct
    24061 tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga
    24121 agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta
    24181 tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag
    24241 agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc
    24301 cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat
    24361 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
    24421 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
    24481 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
    24541 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
    24601 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
    24661 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
    24721 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
    24781 tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta
    24841 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
    24901 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
    24961 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
    25021 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
    25081 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
    25141 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
    25201 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
    25261 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
    25321 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
    25381 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
    25441 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
    25501 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
    25561 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
    25621 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
    25681 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
    25741 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
    25801 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
    25861 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
    25921 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
    25981 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
    26041 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
    26101 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
    26161 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
    26221 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
    26281 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
    26341 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
    26401 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
    26461 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
    26521 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
    26581 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
    26641 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
    26701 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
    26761 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
    26821 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
    26881 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
    26941 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
    27001 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
    27061 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
    27121 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
    27181 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
    27241 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
    27301 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
    27361 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
    27421 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
    27481 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
    27541 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
    27601 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
    27661 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
    27721 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
    27781 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
    27841 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
    27901 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
    27961 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
    28021 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
    28081 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
    28141 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
    28201 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
    28261 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
    28321 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
    28381 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
    28441 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
    28501 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
    28561 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
    28621 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
    28681 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
    28741 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
    28801 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
    28861 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
    28921 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
    28981 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
    29041 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
    29101 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
    29161 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
    29221 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
    29281 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
    29341 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
    29401 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
    29461 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
    29521 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
    29581 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
    29641 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
    29701 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
    29761 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
    29821 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
    29881 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
    29941 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
    30001 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
    30061 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
    30121 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
    30181 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
    30241 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
    30301 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
    30361 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
    30421 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
    30481 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
    30541 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
    30601 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
    30661 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
    30721 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
    30781 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
    30841 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
    30901 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
    30961 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
    31021 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
    31081 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
    31141 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
    31201 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
    31261 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
    31321 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
    31381 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
    31441 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
    31501 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
    31561 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
    31621 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
    31681 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
    31741 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
    31801 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
    31861 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
    31921 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
    31981 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
    32041 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
    32101 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
    32161 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
    32221 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
    32281 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
    32341 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
    32401 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
    32461 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
    32521 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
    32581 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
    32641 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
    32701 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
    32761 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
    32821 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
    32881 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
    32941 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
    33001 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
    33061 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
    33121 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
    33181 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
    33241 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
    33301 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
    33361 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
    33421 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
    33481 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
    33541 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
    33601 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
    33661 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
    33721 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
    33781 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
    33841 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
    33901 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
    33961 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
    34021 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
    34081 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
    34141 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
    34201 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
    34261 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
    34321 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    34381 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    34441 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    34501 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    34561 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    34621 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    34681 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    34741 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    34801 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    34861 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    34921 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    34981 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    35041 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    35101 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    35161 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    35221 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    35281 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    35341 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    35401 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    35461 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    35521 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    35581 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    35641 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    35701 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    35761 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    35821 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    35881 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    35941 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    36001 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    36061 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    36121 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    36181 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaataaga gggacttaat
    36241 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    36301 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    36361 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    36421 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    36481 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    36541 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    36601 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    36661 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    36721 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    36781 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    36841 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    36901 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    36961 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    37021 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    37081 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    37141 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    37201 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    37261 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    37321 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    37381 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    37441 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    37501 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    37561 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    37621 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    37681 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    37741 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    37801 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    37861 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    37921 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    37981 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    38041 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    38101 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    38161 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    38221 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    38281 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    38341 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    38401 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    38461 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    38521 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    38581 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    38641 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    38701 tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc
    38761 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    38821 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    38881 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    38941 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    39001 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    39061 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    39121 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    39181 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    39241 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    39301 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    39361 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    39421 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    39481 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    39541 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    39601 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    39661 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    39721 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    39781 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    39841 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    39901 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    39961 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    40021 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    40081 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    40141 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    40201 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    40261 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    40321 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    40381 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    40441 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    40501 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    40561 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    40621 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    40681 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    40741 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    40801 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    40861 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    40921 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    40981 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    41041 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    41101 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    41161 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    41221 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    41281 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    41341 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    41401 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    41461 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    41521 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    41581 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    41641 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    41701 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    41761 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    41821 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    41881 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    41941 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    42001 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    42061 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    42121 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    42181 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    42241 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    42301 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    42361 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    42421 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    42481 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    42541 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    42601 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    42661 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    42721 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    42781 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    42841 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    42901 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    42961 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    43021 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    43081 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    43141 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    43201 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    43261 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    43321 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    43381 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    43441 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    43501 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    43561 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    43621 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    43681 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    43741 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    43801 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    43861 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    43921 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    43981 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    44041 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    44101 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    44161 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    44221 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    44281 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    44341 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    44401 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    44461 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    44521 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    44581 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    44641 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    44701 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    44761 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    44821 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    44881 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    44941 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    45001 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    45061 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    45121 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    45181 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    45241 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    45301 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    45361 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    45421 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    45481 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    45541 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    45601 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    45661 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    45721 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    45781 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    45841 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    45901 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    45961 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    46021 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    46081 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    46141 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    46201 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    46261 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    46321 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    46381 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    46441 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    46501 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    46561 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    46621 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    46681 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    46741 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    46801 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    46861 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    46921 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    46981 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac
    47041 agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag
    47101 ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    47161 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    47221 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    47281 tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    47341 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    47401 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    47461 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    47521 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    47581 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    47641 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    47701 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    47761 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    47821 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    47881 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    47941 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    48001 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    48061 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    48121 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    48181 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    48241 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    48301 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    48361 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    48421 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    48481 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    48541 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    48601 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    48661 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    48721 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    48781 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    48841 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    48901 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    48961 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    49021 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    49081 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    49141 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    49201 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    49261 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    49321 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    49381 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    49441 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    49501 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    49561 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    49621 catggcttat caatttaaat t
//
LOCUS       DASHWH010000017        47853 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.017, whole genome shotgun sequence.
ACCESSION   DASHWH010000017 DASHWH010000000
VERSION     DASHWH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.017"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(38..1189)
                     /locus_tag="U2R41_000959"
     CDS             complement(38..1189)
                     /locus_tag="U2R41_000959"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEM8888388.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="U2R41_000960"
     CDS             complement(1202..1639)
                     /locus_tag="U2R41_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8888389.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="U2R41_000961"
     CDS             complement(1654..3420)
                     /locus_tag="U2R41_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEM8888390.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="U2R41_000962"
     CDS             3684..4361
                     /locus_tag="U2R41_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888391.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="U2R41_000963"
     CDS             4364..5491
                     /locus_tag="U2R41_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888392.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="U2R41_000964"
     CDS             complement(5691..6131)
                     /locus_tag="U2R41_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8888393.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="U2R41_000965"
     CDS             complement(6142..8175)
                     /locus_tag="U2R41_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8888394.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="U2R41_000966"
     CDS             complement(8177..9880)
                     /locus_tag="U2R41_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8888395.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U2R41_000967"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U2R41_000967"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEM8888396.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="U2R41_000968"
     CDS             complement(10963..11988)
                     /locus_tag="U2R41_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM8888397.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="U2R41_000969"
     CDS             complement(12197..13066)
                     /locus_tag="U2R41_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEM8888398.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="U2R41_000970"
     CDS             complement(13525..15417)
                     /locus_tag="U2R41_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888399.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="U2R41_000971"
     CDS             complement(15414..17141)
                     /locus_tag="U2R41_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888400.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="U2R41_000972"
     CDS             complement(17157..17807)
                     /locus_tag="U2R41_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM8888401.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="U2R41_000973"
                     /pseudo
     CDS             18011..18307
                     /locus_tag="U2R41_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18479..19099
                     /locus_tag="U2R41_000974"
     CDS             18479..19099
                     /locus_tag="U2R41_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEM8888402.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="U2R41_000975"
     CDS             complement(19191..20357)
                     /locus_tag="U2R41_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8888403.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="U2R41_000976"
     CDS             complement(20369..20722)
                     /locus_tag="U2R41_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM8888404.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="U2R41_000977"
     CDS             20850..21872
                     /locus_tag="U2R41_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEM8888405.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="U2R41_000978"
     CDS             21891..22052
                     /locus_tag="U2R41_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888406.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="U2R41_000979"
     CDS             complement(22311..22745)
                     /locus_tag="U2R41_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888407.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="U2R41_000980"
     CDS             complement(22855..24714)
                     /locus_tag="U2R41_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8888408.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="U2R41_000981"
     CDS             complement(24832..25992)
                     /locus_tag="U2R41_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEM8888409.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U2R41_000982"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U2R41_000982"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEM8888410.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="U2R41_000983"
     CDS             complement(27739..28437)
                     /locus_tag="U2R41_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM8888411.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="U2R41_000984"
     CDS             complement(28394..30055)
                     /locus_tag="U2R41_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEM8888412.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="U2R41_000985"
     CDS             30273..31673
                     /locus_tag="U2R41_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEM8888413.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="U2R41_000986"
     CDS             complement(31925..33004)
                     /locus_tag="U2R41_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8888414.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="U2R41_000987"
     CDS             complement(33090..34049)
                     /locus_tag="U2R41_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM8888415.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="U2R41_000988"
     CDS             complement(34061..35572)
                     /locus_tag="U2R41_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM8888416.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="U2R41_000989"
     CDS             complement(35594..36157)
                     /locus_tag="U2R41_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEM8888417.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="U2R41_000990"
     CDS             complement(36163..37026)
                     /locus_tag="U2R41_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8888418.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="U2R41_000991"
     CDS             complement(37040..37936)
                     /locus_tag="U2R41_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8888419.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="U2R41_000992"
     CDS             complement(38111..39400)
                     /locus_tag="U2R41_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888420.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="U2R41_000993"
     CDS             complement(39372..40889)
                     /locus_tag="U2R41_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM8888421.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="U2R41_000994"
     CDS             41087..41977
                     /locus_tag="U2R41_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEM8888422.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U2R41_000995"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U2R41_000995"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEM8888423.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="U2R41_000996"
     CDS             complement(42886..44358)
                     /locus_tag="U2R41_000996"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEM8888424.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="U2R41_000997"
     CDS             complement(44995..45357)
                     /locus_tag="U2R41_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM8888425.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="U2R41_000998"
     CDS             45580..46332
                     /locus_tag="U2R41_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8888426.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="U2R41_000999"
     CDS             complement(46472..47395)
                     /locus_tag="U2R41_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888427.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="U2R41_001000"
     CDS             complement(47410..47682)
                     /locus_tag="U2R41_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888428.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13931 a         9944 c         8258 g        15720 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttaatat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttatgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aat
//
LOCUS       DASHWH010000018        43923 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.018, whole genome shotgun sequence.
ACCESSION   DASHWH010000018 DASHWH010000000
VERSION     DASHWH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.018"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(18..716)
                     /locus_tag="U2R41_001001"
     CDS             complement(18..716)
                     /locus_tag="U2R41_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEM8888429.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(709..2061)
                     /locus_tag="U2R41_001002"
     CDS             complement(709..2061)
                     /locus_tag="U2R41_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEM8888430.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2284..3684)
                     /locus_tag="U2R41_001003"
     CDS             complement(2284..3684)
                     /locus_tag="U2R41_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEM8888431.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3845..4582
                     /locus_tag="U2R41_001004"
     CDS             3845..4582
                     /locus_tag="U2R41_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEM8888432.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4707..5186
                     /locus_tag="U2R41_001005"
     CDS             4707..5186
                     /locus_tag="U2R41_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEM8888433.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5183..5950
                     /locus_tag="U2R41_001006"
     CDS             5183..5950
                     /locus_tag="U2R41_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888434.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6180..6749
                     /gene="lepB"
                     /locus_tag="U2R41_001007"
     CDS             6180..6749
                     /gene="lepB"
                     /locus_tag="U2R41_001007"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8888435.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6853..7455)
                     /locus_tag="U2R41_001008"
     CDS             complement(6853..7455)
                     /locus_tag="U2R41_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8888436.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7561..8030
                     /locus_tag="U2R41_001009"
                     /pseudo
     CDS             7561..8030
                     /locus_tag="U2R41_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8136..8720
                     /locus_tag="U2R41_001010"
     CDS             8136..8720
                     /locus_tag="U2R41_001010"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEM8888437.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8723..9796
                     /gene="prfA"
                     /locus_tag="U2R41_001011"
     CDS             8723..9796
                     /gene="prfA"
                     /locus_tag="U2R41_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEM8888438.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9789..10628
                     /gene="prmC"
                     /locus_tag="U2R41_001012"
     CDS             9789..10628
                     /gene="prmC"
                     /locus_tag="U2R41_001012"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEM8888439.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10621..10800
                     /locus_tag="U2R41_001013"
     CDS             10621..10800
                     /locus_tag="U2R41_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888440.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10822..11847
                     /locus_tag="U2R41_001014"
     CDS             10822..11847
                     /locus_tag="U2R41_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEM8888441.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12022..13266
                     /locus_tag="U2R41_001015"
     CDS             12022..13266
                     /locus_tag="U2R41_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEM8888442.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13419..14366
                     /locus_tag="U2R41_001016"
     CDS             13419..14366
                     /locus_tag="U2R41_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8888443.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMFLTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14500..15129
                     /gene="upp"
                     /locus_tag="U2R41_001017"
     CDS             14500..15129
                     /gene="upp"
                     /locus_tag="U2R41_001017"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEM8888444.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15268..15510)
                     /locus_tag="U2R41_001018"
     CDS             complement(15268..15510)
                     /locus_tag="U2R41_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888445.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15631..15703
                     /locus_tag="U2R41_001019"
     tRNA            15631..15703
                     /locus_tag="U2R41_001019"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15664..15666,aa:Thr,seq:cgt)
     gene            complement(15822..16460)
                     /locus_tag="U2R41_001020"
     CDS             complement(15822..16460)
                     /locus_tag="U2R41_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8888446.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16583..17215)
                     /locus_tag="U2R41_001021"
     CDS             complement(16583..17215)
                     /locus_tag="U2R41_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM8888447.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17566..17865
                     /locus_tag="U2R41_001022"
     CDS             17566..17865
                     /locus_tag="U2R41_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM8888448.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18360..20525
                     /gene="acm"
                     /locus_tag="U2R41_001023"
     CDS             18360..20525
                     /gene="acm"
                     /locus_tag="U2R41_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEM8888449.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20797..21282)
                     /locus_tag="U2R41_001024"
     CDS             complement(20797..21282)
                     /locus_tag="U2R41_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEM8888450.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21372..21717
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21762..22403
                     /locus_tag="U2R41_001025"
     CDS             21762..22403
                     /locus_tag="U2R41_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8888451.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22422..23057
                     /locus_tag="U2R41_001026"
     CDS             22422..23057
                     /locus_tag="U2R41_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8888452.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23075..23890
                     /locus_tag="U2R41_001027"
     CDS             23075..23890
                     /locus_tag="U2R41_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8888453.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24209..25645
                     /locus_tag="U2R41_001028"
     CDS             24209..25645
                     /locus_tag="U2R41_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8888454.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25834..28281
                     /locus_tag="U2R41_001029"
     CDS             25834..28281
                     /locus_tag="U2R41_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM8888455.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28511..29578
                     /gene="rlmN"
                     /locus_tag="U2R41_001030"
     CDS             28511..29578
                     /gene="rlmN"
                     /locus_tag="U2R41_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM8888456.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29771..30874)
                     /locus_tag="U2R41_001031"
     CDS             complement(29771..30874)
                     /locus_tag="U2R41_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8888457.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31427..31975
                     /gene="aac(6')"
                     /locus_tag="U2R41_001032"
     CDS             31427..31975
                     /gene="aac(6')"
                     /locus_tag="U2R41_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM8888458.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32521..34182
                     /locus_tag="U2R41_001033"
     CDS             32521..34182
                     /locus_tag="U2R41_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8888459.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34252..34599)
                     /locus_tag="U2R41_001034"
     CDS             complement(34252..34599)
                     /locus_tag="U2R41_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEM8888460.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34683..35624
                     /locus_tag="U2R41_001035"
     CDS             34683..35624
                     /locus_tag="U2R41_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888461.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35625..36668
                     /locus_tag="U2R41_001036"
     CDS             35625..36668
                     /locus_tag="U2R41_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888462.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36674..37744
                     /locus_tag="U2R41_001037"
     CDS             36674..37744
                     /locus_tag="U2R41_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888463.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37734..38687
                     /locus_tag="U2R41_001038"
     CDS             37734..38687
                     /locus_tag="U2R41_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888464.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38699..39709
                     /locus_tag="U2R41_001039"
     CDS             38699..39709
                     /locus_tag="U2R41_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8888465.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(39994..40815)
                     /locus_tag="U2R41_001040"
     CDS             complement(39994..40815)
                     /locus_tag="U2R41_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8888466.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40912..41190)
                     /locus_tag="U2R41_001041"
     CDS             complement(40912..41190)
                     /locus_tag="U2R41_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888467.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41458..43467
                     /gene="metG"
                     /locus_tag="U2R41_001042"
     CDS             41458..43467
                     /gene="metG"
                     /locus_tag="U2R41_001042"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEM8888468.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT        14956 a         7593 c         8906 g        12468 t
ORIGIN      
        1 tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc caattcttta
       61 ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc atttcttgca
      121 aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc ttcagattgg
      181 tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc accttcacct
      241 aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa ttcttcgtta
      301 tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta gtgatccagt
      361 gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc tagcaattgg
      421 tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat caaagaatct
      481 ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc accgccaaag
      541 aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat ctccgtatgg
      601 caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc gttatcacca
      661 tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt agccacggtt
      721 cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca atacagcagt
      781 gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt cgattacttc
      841 tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt ttagacgtaa
      901 agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca tatcggcaaa
      961 gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt aattggcatt
     1021 taataaagcc actaaagaaa atggataagg agctagacca atcatatcga ttacttgatt
     1081 gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga tattgatgac
     1141 ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt tatcaggatc
     1201 gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat tatacatacc
     1261 gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca cattgtccat
     1321 ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac aatttggaca
     1381 gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt gatgacaatt
     1441 tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt cttcatggtc
     1501 aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg gtgaatcacc
     1561 gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca ttttataggt
     1621 cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa ataagaacag
     1681 ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt ctaggacagc
     1741 aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa ttccctgtac
     1801 catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca aaatattgac
     1861 tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga cttggtagtc
     1921 ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac ctggtaaact
     1981 tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa ctgcaatcgc
     2041 aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac gaataaattc
     2101 aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc ttaagaaaag
     2161 gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa aaaacagctt
     2221 gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac ctatattccc
     2281 attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc gtgtttcttt
     2341 tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca tcagtgtagc
     2401 aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata aaccagttgt
     2461 tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt ctgtagcaga
     2521 taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa cgactgattt
     2581 tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt cacttttgag
     2641 gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc cgccgcctac
     2701 agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg ttgtgatttg
     2761 aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg aataaaacat
     2821 cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct tttccaatag
     2881 ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag ctaatttggt
     2941 aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata gccattcccg
     3001 gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca tgatcaggat
     3061 caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt caaacccaga
     3121 gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac gccaacgatc
     3181 aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta attgaaacca
     3241 gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac gcggttgatt
     3301 catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag aaaaaatgac
     3361 aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata tttccagcag
     3421 tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc cagtcacact
     3481 cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat ctggttcgcg
     3541 aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat aataaaaaac
     3601 gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc gctgcagcct
     3661 tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt ttctaccagt
     3721 atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat acagctttcg
     3781 attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga ggaggaataa
     3841 caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa taaattatga
     3901 tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc ctaagatatt
     3961 aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt ctcaatatgc
     4021 agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt atttgcgaag
     4081 attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc atcatgttgc
     4141 gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa caaaccatct
     4201 tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc gattagatct
     4261 tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc tgaccatctc
     4321 gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa agatttacgg
     4381 gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct ctttgcaaat
     4441 gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg ctgccaagca
     4501 gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa aaacatcaac
     4561 cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa aaataaagaa
     4621 tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag gaaacaagaa
     4681 taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg aagccaatcg
     4741 ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat agttccagct
     4801 accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact acttcagtct
     4861 cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca agtacatggg
     4921 acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc tcaagcgaaa
     4981 caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt acaagtccgg
     5041 atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat aacacgcaaa
     5101 atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc caaacgtata
     5161 accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg gggtgcataa
     5221 attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata cgattttaag
     5281 tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa accaaaacta
     5341 tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta ctgttttaaa
     5401 tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc tttttgtgaa
     5461 gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata atggaaattc
     5521 gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt atcaattgaa
     5581 attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa atgaactcgg
     5641 tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact atgtcaagaa
     5701 aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca tggaaaaagt
     5761 agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat tgttcatcgt
     5821 cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc tgaaattgat
     5881 tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc aaagaagaag
     5941 acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg tttattcgga
     6001 ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga gaaagataag
     6061 acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa agaaagtaca
     6121 acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg aggtaagctg
     6181 tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct attttattag
     6241 ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac cctacattaa
     6301 gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg gatattatta
     6361 ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc ggtatgcctg
     6421 gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag tatgatgaac
     6481 cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga gaatatgctt
     6541 atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt gactttgaat
     6601 atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt tcaaaagaca
     6661 gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt ttccgttatt
     6721 ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg tgacatgttt
     6781 tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc tcactttttt
     6841 tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt ttttgttcat
     6901 aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc ccgttttttc
     6961 caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat gactaatttg
     7021 ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa tttcataaag
     7081 ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata gaaggatgag
     7141 attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc ggacattttt
     7201 cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg aagcatcggc
     7261 aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag ttccgcctcc
     7321 gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta gttcttcgcg
     7381 aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga tggaatcaaa
     7441 aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa cttattatag
     7501 cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc tagaaagaat
     7561 atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg agaaatatta
     7621 ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat cgaggaatca
     7681 agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt tatcactctt
     7741 tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata aaaaaagact
     7801 atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac gcagcaaaac
     7861 attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata tatgatcaaa
     7921 ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca aatttatggg
     7981 atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga gcaatagatt
     8041 taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa atcaagtata
     8101 ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt tttaagtatg
     8161 gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat tatgaagaac
     8221 aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt ttaggcgttg
     8281 tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa acagacgtat
     8341 ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat gaagcgcaat
     8401 tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg gatattccgg
     8461 ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga tcaaaatatt
     8521 tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc tgccacaaaa
     8581 aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc gatcaagtgc
     8641 aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt cacccatcaa
     8701 tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag accgttatga
     8761 agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc gttttatgca
     8821 gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc gttataaaga
     8881 agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg atgatgaaat
     8941 ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag tcttagaaga
     9001 aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata tcatcatgga
     9061 aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc ttttcaatat
     9121 gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag ctaacgttac
     9181 cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg tatattcgaa
     9241 attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg aatcgcaagg
     9301 gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag aagtagagat
     9361 cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg ctggcggaca
     9421 gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag ggatcgttgt
     9481 tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga aaatcttgcg
     9541 tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg ccaatcgaaa
     9601 atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt tcccgcaaaa
     9661 tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga ttttagcagg
     9721 gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa aactggaagc
     9781 tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct tcttttttag
     9841 aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga aaacaatgga
     9901 aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa cagcggttga
     9961 tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg ctaggatatg
    10021 cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct cgaccagaaa
    10081 cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta gaagtgatag
    10141 atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga aaaaattggc
    10201 atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa aatgctcaga
    10261 aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta atggatcagc
    10321 aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg gaactgatgg
    10381 atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac gatggattag
    10441 ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat ggaaagattt
    10501 ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag caagcatttc
    10561 cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg atccaagttt
    10621 atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa aaaacactta
    10681 tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact attgacaaat
    10741 agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa gaatgtatga
    10801 aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga attgcaagag
    10861 gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac agtatacgga
    10921 ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt aaaagggaga
    10981 ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa agagtatgtg
    11041 gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg accattaact
    11101 ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg attatccaca
    11161 gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact ttccggtgtt
    11221 ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac tgcagatcat
    11281 gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc tacgcgaatt
    11341 ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat gattttacga
    11401 ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt tgctattgat
    11461 cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata caaacactac
    11521 tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc agttcaatgg
    11581 gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc ggatcaagtg
    11641 cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc ggcggctaaa
    11701 ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga tttgattttt
    11761 gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt gaaaaaagca
    11821 gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct tttagtatga
    11881 tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta actaatcgta
    11941 atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa attgtaggaa
    12001 aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga tctttgggct
    12061 gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc ctctgaaaac
    12121 tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa atacgcagaa
    12181 ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt tgaaaattta
    12241 gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca gcctcactcc
    12301 ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga cacgatctta
    12361 ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa cttcagtggc
    12421 aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat tgattataat
    12481 gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg tgccagtgct
    12541 tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt aggcgcaaaa
    12601 ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca tcctaatcca
    12661 gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg ccctcgaggc
    12721 ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc ggtatttcct
    12781 ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc attcaaagaa
    12841 gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc aaaagcaatg
    12901 gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac agataatcat
    12961 ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga gagcattttg
    13021 gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact aagtccattc
    13081 aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt caaagaagaa
    13141 gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga tgataacgct
    13201 caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc gttgcataaa
    13261 aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct tttctgttta
    13321 ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat atgaggtgta
    13381 attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt atctcgttag
    13441 aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc tatgaactag
    13501 caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg aacaaatatt
    13561 ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc aatattcgtc
    13621 tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt catggagcag
    13681 gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt gcggttcgta
    13741 cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa tatccaacag
    13801 caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag tcaaaaaatc
    13861 tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg gctacagtca
    13921 tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg ctgtattatc
    13981 cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa aactattttt
    14041 tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa gaagagcgtg
    14101 ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat cttccttcag
    14161 caatgttttt aacgggagaa gcggatgtat taagagatga aggtgaggct tatgcgcgca
    14221 aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc atccatgatt
    14281 ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg gcattatcta
    14341 cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact tcttttcaaa
    14401 aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat tttgttttac
    14461 aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt tcaagtgatc
    14521 gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg cggaacaaag
    14581 gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt atcacgggat
    14641 atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca aaaaacgttg
    14701 tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat ggttgacggt
    14761 atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg agatgaagaa
    14821 acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc acgtcagttg
    14881 tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat cgatgcattg
    14941 aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc tgagggagta
    15001 aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct agatgaaaaa
    15061 ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg tttgtttggt
    15121 acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc ttttttggtc
    15181 atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata taaaaaaacc
    15241 atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt agttaacgat
    15301 tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat caatctcaac
    15361 aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc cgtgaattgt
    15421 gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta cgatagttgt
    15481 atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca attataccac
    15541 gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt acaatcagcc
    15601 aaatagagta tataatttct tttggataag gccagcttag ctcagttggt agagcaacgc
    15661 actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag agaagacaga
    15721 tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg aaataaagta
    15781 tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa taaaacgact
    15841 agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat gatcgagttg
    15901 cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa aaggatgtgt
    15961 gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa tccaaagatg
    16021 attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat agcgagaaaa
    16081 tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa taataacggg
    16141 ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg ttgcaaaaat
    16201 accgagactg cggcactgat tactacattg ataaaaaaat atttggctat atttttgaca
    16261 ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac aagtgtaaat
    16321 gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc aaatagcaac
    16381 cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag ttctattttt
    16441 tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa gatagagtca
    16501 gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat tgctaaaaat
    16561 ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt ctagcaaaac
    16621 caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt acttcaatca
    16681 tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa aagctagcat
    16741 tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca aaatctgcgc
    16801 ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat aaatttacat
    16861 agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc aatttagtac
    16921 ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc aatatacagc
    16981 ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga aaatcattgg
    17041 aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag attatttggt
    17101 tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg ttttcataga
    17161 tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa atcatattta
    17221 attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac attagcagaa
    17281 aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt gaaacgttct
    17341 tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt agaataaaaa
    17401 tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata acagtgtatt
    17461 gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt atactttgtt
    17521 tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc taaaagtgaa
    17581 gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga gatcaaagaa
    17641 aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca gtattatcct
    17701 aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg agaattgtct
    17761 aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga aaaagaatta
    17821 ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg agttattcat
    17881 ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat ttatttgaac
    17941 agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat tgtattttat
    18001 cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa agtgtatttt
    18061 atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg gaaatataga
    18121 ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa taatttacat
    18181 taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa tatttgtaaa
    18241 ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat tgaagaaagg
    18301 atttttgaga gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa
    18361 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
    18421 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
    18481 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
    18541 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
    18601 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
    18661 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
    18721 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
    18781 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
    18841 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
    18901 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
    18961 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
    19021 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
    19081 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
    19141 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
    19201 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
    19261 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
    19321 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
    19381 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
    19441 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
    19501 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
    19561 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
    19621 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
    19681 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
    19741 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
    19801 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
    19861 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
    19921 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
    19981 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
    20041 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
    20101 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
    20161 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
    20221 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
    20281 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
    20341 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
    20401 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
    20461 tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag
    20521 tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg
    20581 atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa
    20641 tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg
    20701 ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag
    20761 acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag
    20821 atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt
    20881 attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac
    20941 ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac
    21001 gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg
    21061 ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg
    21121 ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg
    21181 gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt
    21241 ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt
    21301 ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg
    21361 taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct
    21421 ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga
    21481 agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag
    21541 ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc
    21601 ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg
    21661 ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca
    21721 tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag
    21781 ttatgccgca attattagta ggagcactca caacattgaa attattttta tttaccttga
    21841 ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc
    21901 tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac
    21961 tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag
    22021 cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga
    22081 tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc
    22141 aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga
    22201 atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg
    22261 taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg
    22321 tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag
    22381 gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag
    22441 tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg
    22501 ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct
    22561 tggctggctt agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc
    22621 cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat
    22681 ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga
    22741 caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt
    22801 tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca
    22861 gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc
    22921 ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc
    22981 aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag
    23041 tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc
    23101 tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa
    23161 ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc
    23221 tatgggattt agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc
    23281 agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga
    23341 atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc
    23401 aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac
    23461 acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca
    23521 aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt
    23581 caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag
    23641 cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag
    23701 aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt
    23761 tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact
    23821 agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc
    23881 gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata
    23941 aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct
    24001 tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta
    24061 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
    24121 cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga
    24181 ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa
    24241 agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga
    24301 tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg
    24361 aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat
    24421 gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa
    24481 tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt
    24541 gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt
    24601 gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc
    24661 agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca
    24721 aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc
    24781 gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa
    24841 atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta
    24901 tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc
    24961 caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt
    25021 agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc
    25081 taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt
    25141 aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac
    25201 tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga
    25261 ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg
    25321 catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa
    25381 acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga
    25441 agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct
    25501 tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac
    25561 acaagaagga tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat
    25621 tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata
    25681 tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg
    25741 aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg
    25801 tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa
    25861 aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg
    25921 attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt
    25981 ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc
    26041 tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt
    26101 ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta
    26161 catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg
    26221 gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg
    26281 attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta
    26341 gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag
    26401 ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct
    26461 gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg
    26521 aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag
    26581 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
    26641 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
    26701 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
    26761 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
    26821 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
    26881 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
    26941 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
    27001 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
    27061 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
    27121 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
    27181 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
    27241 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
    27301 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
    27361 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
    27421 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
    27481 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
    27541 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
    27601 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
    27661 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
    27721 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
    27781 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
    27841 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
    27901 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
    27961 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
    28021 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
    28081 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
    28141 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
    28201 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
    28261 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
    28321 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
    28381 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
    28441 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
    28501 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
    28561 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
    28621 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
    28681 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
    28741 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
    28801 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
    28861 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
    28921 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
    28981 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
    29041 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
    29101 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
    29161 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
    29221 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
    29281 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
    29341 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
    29401 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
    29461 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
    29521 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
    29581 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
    29641 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
    29701 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
    29761 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
    29821 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
    29881 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
    29941 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
    30001 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
    30061 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
    30121 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
    30181 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
    30241 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
    30301 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
    30361 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
    30421 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
    30481 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
    30541 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    30601 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    30661 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    30721 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    30781 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    30841 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    30901 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    30961 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    31021 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    31081 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    31141 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
    31201 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
    31261 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
    31321 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
    31381 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
    31441 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
    31501 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
    31561 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
    31621 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
    31681 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
    31741 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
    31801 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
    31861 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
    31921 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
    31981 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
    32041 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
    32101 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
    32161 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
    32221 attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt
    32281 atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc
    32341 atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa
    32401 tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac
    32461 tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa
    32521 atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt
    32581 tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga
    32641 ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca
    32701 gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa
    32761 gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta
    32821 acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca
    32881 gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct
    32941 tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca
    33001 gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca
    33061 ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg
    33121 gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc
    33181 acattagaag attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat
    33241 cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa
    33301 tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt
    33361 gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca
    33421 agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga
    33481 aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga
    33541 tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa
    33601 gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat
    33661 tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct
    33721 gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg
    33781 gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact
    33841 tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc
    33901 tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa
    33961 tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg
    34021 gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac
    34081 caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca
    34141 ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca
    34201 tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa
    34261 caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca
    34321 aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg
    34381 gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa
    34441 gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga
    34501 taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag
    34561 tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg
    34621 gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat
    34681 tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt
    34741 ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga
    34801 atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata
    34861 aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa
    34921 tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat
    34981 ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa
    35041 cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat
    35101 taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca
    35161 aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa
    35221 ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg
    35281 aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa
    35341 tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt
    35401 tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta
    35461 tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa
    35521 cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc
    35581 tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa
    35641 agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata
    35701 ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc
    35761 gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt
    35821 tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt
    35881 atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa
    35941 tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt
    36001 tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga
    36061 gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt
    36121 acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg
    36181 aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac
    36241 caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta
    36301 tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat
    36361 taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca
    36421 ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct
    36481 ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt
    36541 acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta
    36601 tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga
    36661 aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac
    36721 gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac
    36781 attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg
    36841 cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat
    36901 cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt
    36961 ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc
    37021 ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct
    37081 taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc
    37141 aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct
    37201 gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct
    37261 gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg
    37321 tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt
    37381 tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg
    37441 ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc
    37501 ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa
    37561 aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac
    37621 ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg
    37681 ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa
    37741 ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag
    37801 tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg
    37861 gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta
    37921 ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt
    37981 tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc
    38041 ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat
    38101 catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa
    38161 gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg
    38221 cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag
    38281 atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat
    38341 tgacctatct atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg
    38401 gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg
    38461 gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg
    38521 agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac
    38581 ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg
    38641 caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat
    38701 gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg
    38761 tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg
    38821 gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc
    38881 attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc
    38941 ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat
    39001 gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt
    39061 aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc
    39121 gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg
    39181 tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc
    39241 gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac
    39301 agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca
    39361 tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga
    39421 tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt
    39481 ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga
    39541 tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta
    39601 taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa
    39661 gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga
    39721 aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct
    39781 aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag
    39841 aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat
    39901 acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt
    39961 gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg
    40021 atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg
    40081 gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt
    40141 agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg
    40201 cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg
    40261 gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc
    40321 cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac
    40381 ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac
    40441 tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat
    40501 attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc
    40561 agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg
    40621 cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag
    40681 tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca
    40741 gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga
    40801 agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta
    40861 gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg
    40921 aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt
    40981 ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa
    41041 acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc
    41101 atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact
    41161 aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata
    41221 gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa
    41281 caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta
    41341 gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt
    41401 tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg
    41461 gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat
    41521 atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg
    41581 ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa
    41641 gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag
    41701 aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac
    41761 cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta
    41821 ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt
    41881 gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac
    41941 gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt
    42001 ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa
    42061 atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt
    42121 acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca
    42181 ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa
    42241 tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat
    42301 tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc
    42361 tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa
    42421 atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg
    42481 tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg
    42541 gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat
    42601 ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg
    42661 gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca
    42721 ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca
    42781 tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta
    42841 gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag
    42901 aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg attacacttt
    42961 ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta
    43021 gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac
    43081 gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa
    43141 atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa
    43201 aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac
    43261 cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa
    43321 aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga
    43381 atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc
    43441 atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca
    43501 tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag
    43561 ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg
    43621 agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa
    43681 aagcataatg ataaagggtc gggcagattt taattgattc atctggtact cttttttatt
    43741 gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac
    43801 agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct
    43861 tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg
    43921 ttt
//
LOCUS       DASHWH010000019        39870 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.019, whole genome shotgun sequence.
ACCESSION   DASHWH010000019 DASHWH010000000
VERSION     DASHWH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.019"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(166..651)
                     /locus_tag="U2R41_001043"
     CDS             complement(166..651)
                     /locus_tag="U2R41_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEM8888469.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(653..2089)
                     /locus_tag="U2R41_001044"
     CDS             complement(653..2089)
                     /locus_tag="U2R41_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEM8888470.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(2122..2973)
                     /locus_tag="U2R41_001045"
     CDS             complement(2122..2973)
                     /locus_tag="U2R41_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEM8888471.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(3129..3374)
                     /locus_tag="U2R41_001046"
     CDS             complement(3129..3374)
                     /locus_tag="U2R41_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888472.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3479..4252
                     /locus_tag="U2R41_001047"
     CDS             3479..4252
                     /locus_tag="U2R41_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888473.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(4305..4619)
                     /locus_tag="U2R41_001048"
     CDS             complement(4305..4619)
                     /locus_tag="U2R41_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888474.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6138..6356)
                     /locus_tag="U2R41_001049"
     CDS             complement(6138..6356)
                     /locus_tag="U2R41_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888475.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(6371..8998)
                     /locus_tag="U2R41_001050"
     CDS             complement(6371..8998)
                     /locus_tag="U2R41_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888476.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(8985..9203)
                     /locus_tag="U2R41_001051"
     CDS             complement(8985..9203)
                     /locus_tag="U2R41_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888477.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(9200..10075)
                     /locus_tag="U2R41_001052"
     CDS             complement(9200..10075)
                     /locus_tag="U2R41_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888478.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(10340..10639)
                     /locus_tag="U2R41_001053"
     CDS             complement(10340..10639)
                     /locus_tag="U2R41_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888479.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            10841..11638
                     /locus_tag="U2R41_001054"
     CDS             10841..11638
                     /locus_tag="U2R41_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888480.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            11689..12831
                     /locus_tag="U2R41_001055"
     CDS             11689..12831
                     /locus_tag="U2R41_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8888481.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWSDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(12929..14320)
                     /gene="sufB"
                     /locus_tag="U2R41_001056"
     CDS             complement(12929..14320)
                     /gene="sufB"
                     /locus_tag="U2R41_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEM8888482.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14325..14795)
                     /locus_tag="U2R41_001057"
     CDS             complement(14325..14795)
                     /locus_tag="U2R41_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEM8888483.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14782..16017)
                     /locus_tag="U2R41_001058"
     CDS             complement(14782..16017)
                     /locus_tag="U2R41_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8888484.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16014..17300)
                     /gene="sufD"
                     /locus_tag="U2R41_001059"
     CDS             complement(16014..17300)
                     /gene="sufD"
                     /locus_tag="U2R41_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEM8888485.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17313..18086)
                     /gene="sufC"
                     /locus_tag="U2R41_001060"
     CDS             complement(17313..18086)
                     /gene="sufC"
                     /locus_tag="U2R41_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEM8888486.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18283..19131)
                     /locus_tag="U2R41_001061"
     CDS             complement(18283..19131)
                     /locus_tag="U2R41_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM8888487.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19155..19841)
                     /locus_tag="U2R41_001062"
     CDS             complement(19155..19841)
                     /locus_tag="U2R41_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888488.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19834..20868)
                     /locus_tag="U2R41_001063"
     CDS             complement(19834..20868)
                     /locus_tag="U2R41_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888489.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20931..21187)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21263..21601)
                     /locus_tag="U2R41_001064"
     CDS             complement(21263..21601)
                     /locus_tag="U2R41_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEM8888490.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21603..21959)
                     /locus_tag="U2R41_001065"
     CDS             complement(21603..21959)
                     /locus_tag="U2R41_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEM8888491.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21965..23152)
                     /locus_tag="U2R41_001066"
     CDS             complement(21965..23152)
                     /locus_tag="U2R41_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM8888492.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23343..24188)
                     /locus_tag="U2R41_001067"
     CDS             complement(23343..24188)
                     /locus_tag="U2R41_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8888493.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24422..25387
                     /locus_tag="U2R41_001068"
     CDS             24422..25387
                     /locus_tag="U2R41_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEM8888494.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25764..26807
                     /locus_tag="U2R41_001069"
     CDS             25764..26807
                     /locus_tag="U2R41_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEM8888495.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26869..27333)
                     /locus_tag="U2R41_001070"
     CDS             complement(26869..27333)
                     /locus_tag="U2R41_001070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEM8888496.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27579..28526
                     /locus_tag="U2R41_001071"
     CDS             27579..28526
                     /locus_tag="U2R41_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8888497.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28737..30809)
                     /locus_tag="U2R41_001072"
     CDS             complement(28737..30809)
                     /locus_tag="U2R41_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8888498.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30742..31119)
                     /locus_tag="U2R41_001073"
     CDS             complement(30742..31119)
                     /locus_tag="U2R41_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEM8888499.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(31248..31826)
                     /locus_tag="U2R41_001074"
     CDS             complement(31248..31826)
                     /locus_tag="U2R41_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8888500.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31958..33250
                     /locus_tag="U2R41_001075"
     CDS             31958..33250
                     /locus_tag="U2R41_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8888501.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(33310..34428)
                     /locus_tag="U2R41_001076"
     CDS             complement(33310..34428)
                     /locus_tag="U2R41_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEM8888502.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(34442..35863)
                     /gene="arcD"
                     /locus_tag="U2R41_001077"
     CDS             complement(34442..35863)
                     /gene="arcD"
                     /locus_tag="U2R41_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEM8888503.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(36109..37230)
                     /gene="mnmA"
                     /locus_tag="U2R41_001078"
     CDS             complement(36109..37230)
                     /gene="mnmA"
                     /locus_tag="U2R41_001078"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEM8888504.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37634..38233
                     /locus_tag="U2R41_001079"
     CDS             37634..38233
                     /locus_tag="U2R41_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEM8888505.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(38371..39657)
                     /gene="efmA"
                     /locus_tag="U2R41_001080"
     CDS             complement(38371..39657)
                     /gene="efmA"
                     /locus_tag="U2R41_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEM8888506.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT        11490 a         8154 c         6655 g        13571 t
ORIGIN      
        1 ggattaaaca ttttttatca tagagtttta cttatctata gaattatgtc tgttcctaat
       61 tcagtttgat gaattaactg ataaaaaaat atctactatt taaagataca gggataagta
      121 agtaaaaaaa taacatgtca ttagatagta gacagttcca actctttatt tacctttttt
      181 gtatgctgca ctgtcaatcc aagttcctaa attgttgtat ccatcatcac ttatataatc
      241 atattggcca tcaataattt cgtcaaacca caaatattct ccatctggtt tttgcccaat
      301 tattttaatt actgattgac tagtagttgt catctggtta aaatctttta ttttacctat
      361 attaacggcc aaaatagcat ttcctcgctt ataactctcc tggatttcat atttaacata
      421 ttctcgatca agagtttctt tacctatcag caccacagtc actgatgttc caattaattg
      481 attatctatc cagtttttta tagcaatatc tccattacgt ttaactttct caaattcagc
      541 cttatctaca aaaccagctt cctttgttcc tcgcaaagtc cagctgtttc taactaccat
      601 tgctcttgtt acatctgcat aatggaaact aaaaaaaacc cttctcacca tattaatcct
      661 cctgaatact atttattaaa ctgacaattt gttcaacttt tttatctact tctccttcag
      721 tatctaattt ttctagcgct tgaaatttac tttttaagga atcatcaaaa taatcactga
      781 aattctcttg aatcttatca aacaaaacct tacttgcacc tttggtaaat ccaatcggaa
      841 ctactatatt ttcattttga acagataatt caaattcttc aatcattcca tctgctgtaa
      901 ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat actaatccca gactctgcaa
      961 taatgttttc tcgatatctt ctccactcat gaggcatatc aaggggaagt ggtcttaaag
     1021 aaagtacgtc ttgtatgtta ttttttctta aatttcttct ctgctttaga actcctgaaa
     1081 caatactatt accaattcca agtccgtaac ctgatactat tctataaccg gattcagaaa
     1141 gcctatatgc taatttctcg acaaatttta atcctttcga ttcagatgta aatggagtat
     1201 aatcaacagc acttcccgaa ataaaaatag tttttctcaa atactttttt ctaatacttt
     1261 tcaaaatatc agtaatctca gaataatcat ctacttcaac aacgtttatc gaaaatctgg
     1321 acaaatcatt gatttgtagt cttctttttg tcagttcata attatatctc tcttgatcaa
     1381 aaaaatcctc agaatcgaca aaattttcct ttaatatttt tctcattata caataatgag
     1441 gtctttggtt ttctctcaaa acccatctta tatctgataa aattctttct aaattagggt
     1501 ctgtaaaact aaatcctaaa aagagaaaag tttttgttaa caaatctcct tttaaagttt
     1561 ctttaaataa cgcgtacgaa tcatcatcat atttttcata atcatctctt gttattactg
     1621 tcgaactgac atcatctata tcaccatgca ttttataaac aattgtatcc acatttggat
     1681 tagttgttgc taactgagta taatttttct taacctgagg gtttttataa gcttctcgga
     1741 ttgatttttc tattagatga tcataattag ttgtccaata agtttcaata ggtaaacttg
     1801 ctagtattcg atgattttca gatatttcta attcttcacc aaaattatcc ataagtatct
     1861 gattcaaaat tcctcttgat ccaccatttt catttttata atattgagcc agagtaacta
     1921 agtctgtttc ttttttagaa tccaacccga tttctgttgc aggatcatta agtagttccg
     1981 gccaacttac atatccttgt gacatcgata atcctgcacc tgcaaatatt gctgcatttc
     2041 ctttttcaat agcttcagag aacctcttta ttaaaacttt cttatccatt ctacccggtt
     2101 cctttcgatt cagtaaattg attagagatt agtgaaaatg gtgtttttat ataaaatatc
     2161 taatgtatta cttcctttag tgcattccac agcatctaaa gcccattttt tcaaatcagc
     2221 ttttcgtaaa taagttaatt cacttaaatt taaagaatat tttatttgca gattttcttg
     2281 cgaagaatag tgctttataa tgcttctaac atcctcggtg ctcatttgag gattactttc
     2341 caatcgtgga ttcttttttt ccactattga agctgtcgtc agttcgctat atagccatgg
     2401 cgaatacgta aatttctgtt cattagtgat ttcattgctc aaactaattg aattaggtgt
     2461 attaagaaaa aacagacatt ctgtactatc aatcatatta tttaaagctg tagaaagcat
     2521 catatgaaca tgactagttg ttacatttct tttatcataa tcatatgttt ttgtttgttc
     2581 cttatagcaa tatttttggt ctattttttt taataaatca gaactgtatc cccaaaccga
     2641 agaatcaata aatgcagtaa gaccaaaatt ttcaaatagc catgatgcaa aacgtatagc
     2701 taatttttca tcagcatgag agtgagatat aaaaatatct gcttttatag ttggaaacca
     2761 atctttaata atttcgtctg catctagaat tttgctgtcc ttagaataat aatcaactaa
     2821 atcattttca ctttcaaata gattttctat tctcttgtta atagattttt ggttgtgatc
     2881 tatttgattt actacatgtg tatatgattg cgagcttgag gagaatgcat ttaagtcagc
     2941 atttgtaaaa tccaattcat atgccttata caaggatatc cctccattct tgtattttga
     3001 tccacaattt attaggcaac ataacagtat accctatcgt tacatatccc attcattatt
     3061 ataccagtat ttcttcgtga cacataaggg tgtaacataa taatttcaaa cgaaatgact
     3121 acaagtagtc attgaagcaa atcttcataa gacacctgta gaatatcttt gatggctttc
     3181 aattgatcca acttaatgtg ttggcggcca ccttcaatct taaccaacgt ttccctagtt
     3241 atagcaattt tacgaagctg taggtctcta atcagctcgg tttgaccaat tcctttttct
     3301 aatcggactc gtctaatatt gctaccgatt acattttctt tatcaataat tttttcctct
     3361 ttcaagaata gcacaccttt tggactaaaa ttagtccttt ttcttattaa tcttaaagat
     3421 tcggtatgct acaatgggac tagtattagt ccatattcaa ttttaaggag gaattttttt
     3481 gaagaaattt gcaatcattc taacttgcgc tttactcatc ggaactataa gcgggtgtac
     3541 cacttctgac acaagttcta caagtacgga aactagtaca acaacctcag aaaagaatta
     3601 cccaatacat ttttttccgg ttccagatgg atttgaagct tattatcatg ataaaaaact
     3661 aaatgttcct ttatcagaaa ctgaatctac tgaaaagatt ttgccaattg aaattgaaag
     3721 aaatcaagac gatataaaac taaaagctga tcttccctta ctgaacagca cacaaattga
     3781 tagacatgaa aatattaaag ttaatattga aaaaggagcg ttagcacaac tttatactga
     3841 aaaagttgaa caattttttg aggctggtgc caataaagac ttttcaaaaa taagcgatta
     3901 ttcggataac ttttttaatg agacccaaga tgaaatcacg aactcaactt ttatggctga
     3961 agatatgtac aatatgaagt tgaaaaaaat aaagtcgtat gattatccat taaatctaac
     4021 attaaataaa gggatactct cttttgtatt agcaggttac gttgactatg aatatacagc
     4081 agattatttg gagacgcccc ttcaacacga taataacgat attaatttta gatttatcta
     4141 caattcaaaa agtaaaacat ggttagttga tcaattgagc actataaata cgggatttca
     4201 tgaattacct aagcctgaca atgaaactgc tttggtcatt aaaacattct aaaaaagaaa
     4261 cgttgaagga ataattattc tttcaacgtt tcttttattg tttttcagtt tacggtctta
     4321 cctgagcgaa taatctgatt tctaaatgat tggaaaactt cttgtggttg ataataatct
     4381 gtatctattt caaatgtttc tgagaataat tcattgaatt tttctactga aatgatgatt
     4441 ttaccattct cctctcgact gttttctatt atttctgcct ctaattcaaa taaatctagt
     4501 gacgaactaa aatcataaat cctgtcatat tgaacaaaaa aatcaagaaa aagttgctca
     4561 aactcttcct tatttatcac tagtgtttca agcggaacaa ctttattttc atcagccatt
     4621 cttccaattt cctcactttc attttcaatt gtccattgtt gcggacgtat cagattttct
     4681 ctatcatttt tcagtgtata gatcacttgt aatacacaac aatgctttgt aaacttattt
     4741 ttttagttag tcgaatcttt ttaaatgtgc gtttcatacc atgcgcttga tatattttct
     4801 cctatttttt aaatcgttta tcacaacttt tatcgttttt tcttctgaaa aaaatcttgt
     4861 ttacatcgtt acatagtttc caaaaagata cattactagt agaaacaaca agattaattt
     4921 tacttcccac tgaaatggga agtatatttt aaatattttt tctccttcaa cttacactat
     4981 aaagtatctt aatcacacga tatgctagcc actaaattca tcatatttga ttcctttttt
     5041 aagataatgt agcatctttt tatccaataa cgcaattgtt tttaaggata aatttcttaa
     5101 gatggtacat ttcaaaatgg catttgacat atgaaacaaa aaagaaagaa aagagcataa
     5161 gggaaaactg atagcacctt cctacaggaa ggtggctaag ttggctgtgc caactggttt
     5221 tctttcaaaa tcatttcata atttttgata tcataaaaaa atccattttg gacctatttt
     5281 cggtcataat ttagtaccta cttttggtca tagtttcgtc cgtagttttg gtcatagtga
     5341 ctaaggcttt tctgttcaca aaattggttt aaatttgctt tctttaacac ttcatttata
     5401 tcatagcaat tataatgttt acacaacatc attcctaatt ttaacggttg ttcctcttta
     5461 tattttttaa gttctacatt tttttgcgtt taattacttt tctcttttct ttatattccg
     5521 ctccaacttt ctctttaata gtgataatag aattacatat ttttgttaaa cttccttaaa
     5581 aaaattggat ctcgattact caaatttgct tgcaggcttt tctaattgct ttgttttttc
     5641 ttcctaatta tttgatttta tttccatttt cttctagtga aaatttatta tttttaataa
     5701 tcgtacgcgt agaactgatt tcattttttg tatctgtttg attatctaac gtttgaaatt
     5761 caaagttttg attggcttta tcaatatctt tttatactac tttatttgca agatatattt
     5821 tcttgcttaa tttggtcata gtttcattcg tcacttcggt gatatttttt taatttgcaa
     5881 ataaaaagag ttaaaccgat atacattagg ttcaactctt ttgcaggttt catgggtgta
     5941 ctatcacttt taatctgata gatgtaaacc tctctttatc tttttgttac ttcaattttc
     6001 aacaatagct cctttatagg ttgtcaatat tgctctacgt attctggctt tttaacccat
     6061 tatttctcta aagtggaaga gaataatcgc ttaatcccca ctaattcctt gtcgtaaatt
     6121 catgtcaaat acaattttta aaagccattt aattcatgga ttatctcttc acttttttct
     6181 agataacaat atttcaagtt gttttttatc tcatgtttca tctttaattc ttcttgaatc
     6241 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     6301 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa tttcatgaca
     6361 attccccctt ttattttttt aatagattat cgaaaagcgg attaggatca ttaagtgtat
     6421 ctctgaataa ctcgttgtct tcataggtaa atcttcgtct tggttttgat cccttatcat
     6481 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     6541 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     6601 tttgaacgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     6661 cattctctct tacatccatt gatttaaacc cttgactact tgtagaattt tgtacattct
     6721 ctcttacatc cattgattta aacccttgac tacttgtaga attttgtaca ttttctcgaa
     6781 catcaattgt cttagtacct tgaccatttg aagagctttg taaattctct cttacatcaa
     6841 cattccgtcc gacatgacca ttcaatgaac cttgaatatt ttctcggaca tctactgatc
     6901 ggccaccttg actacttgta gaattttgta gattttcttt gaccaatttt tctaatccgg
     6961 tatttgagtt tttagcttct tggattcttt ctttggtcaa cgcttctttt tcgtccgtat
     7021 tagctcctgt acgcccttct acacgtttcc ttaacatttc ctcacgattc attgcttcag
     7081 gcgtgtattt agcttcttgt cgcttctgat taattaattc ttcagcagac ttagcttgac
     7141 cgtgcatagc atttttacgt tcttcaatat cttgttgaac tttttgctga attgcactta
     7201 caggattatt attcgattca gcattatttt tcattgcttc acgcatacgt tcagaactag
     7261 acataattct atcggcattt tcaaccttag caccatcttt tctatccgaa agacgtgttg
     7321 gcatggaatc agcatatttt ccagtttcat cttctttatt tttagctaac ataggtttgc
     7381 tatttttatt aatagcggac actgtaccat catctaaacc ttctttgaac ttattcttgg
     7441 tatcattagc catatcacgc atactcttaa atggttttat tgctctttca ctagccattg
     7501 tagctccatc tgaagctaaa cctgataatc cacgttcatg ggcatatccc aaagctcttc
     7561 cagttttgcc agcttttttt gatagagttt cgccgaaatt cttttctggt tttcgatctt
     7621 ctccttctcc actgtctgat ccatttccat ttccgcttcc tttagcacga ttaaatccag
     7681 ttgatccttt tctaaacagc attgcgccca taccaaaagc agaagcaagt tgcccatagc
     7741 cttctttgag tccaatatca acaccaaagt atctcatgac tgttccactg ccttttatca
     7801 gcacaaacac agcacagata taagcaattg tttttataaa aatatttgtg gagatacctt
     7861 gtccagcatt taaaaaattg ataaacatag caaacataga tagtccaaac ccttgaaatc
     7921 caactgttaa gtaacattgt agaatatctg atagaacaac ttttgaacgt tgtcctgttt
     7981 caatatctgt cgcaaaaaca agaacaccta acacacgttt aaatgcaagt tctaatattg
     8041 ctgtaatgat cacaaacgcg ctgaacacat aggcgattgc caacgctgtc aacccaatcg
     8101 aaatccccca aagatttgct tgatatcgat aataacctga tttcaagcta tcagaaaatg
     8161 ttgataagaa gttatcagaa aactttgtgg caacaaattc attattagaa ttttcaacta
     8221 attcatagcg aagattatcc gcagctggat tttctttttc caatttatca atcactttat
     8281 ccgtcaacac ttgtgttaaa tctgtctgtt gaaaattttt tcgtgttaac ttatttcgat
     8341 cctccgtttt atcaatgctt gagtattgat ctgttttatt aatatatgcc agatctgtta
     8401 ctccttgttt tatcaatgac caagcgactc cgtcatctga atttgtaatc gtagtagcat
     8461 cattgtaaaa tattttggca aattgaatac cgctagaaat cattgttggc attaaaagaa
     8521 ttaacgctac agacatcact atattcattc caacagatct taaatctatt gttccttttc
     8581 cagtaatcat cttgtagcca acaaacatca atgaaaggat cattaatcca acgacaattg
     8641 tatttatgac tgattgataa acattattta gccctgtact ttcaatgaaa ttgaacaaat
     8701 ccaacacatc tggaatcata ttttgaatcg aatcagtcac agtaaacatg ccttttacca
     8761 cagcccatcc aagccaaatc ccgaactgac taaggaaatc tccattgtat ttttttaggt
     8821 aaggtctata aagagaatta ttcaaaaact ctgtttgact ttttgtgtca tcaaaccaat
     8881 tgaaaatgct agcttcaaca atattacttg taaaaacaac gctaatcatt aaaacaagta
     8941 cactgagtgc taaccagttt atttttctac taacgatttt ttctttaatt gctttcatag
     9001 ttttctcctt cagcccattt tcttaggctc tcagagagct gctgagccat tttttgatta
     9061 attatgatat gagttacaca attgtcattt tcagctctta aatcgagaac tactgtgttg
     9121 aagcagatac tctcgattaa ttcgagagta tctgtatttt gatcaagaca ccacatttga
     9181 tctattaagc gatttttaat catgattctt ctcctttcaa ccaattatgc atagtaattt
     9241 caggtaactc tttgatttcg aaagcttttt tgaactgatc tataggctct acattttcta
     9301 aaaattgact tttatatctt ttctgaagtc cattcaaaaa atatgttaca taatcgtata
     9361 acccttcttc agtttttctt tgaaaaaagc atgcttcatg ttgttttata atacactcag
     9421 caataagata ctgagtattt tcagataaat attcaatgat ttttctgata tcttctgtat
     9481 ctacattagc gtttctcaaa tagtgataca cttgaaataa ttttcgacca tcttcgttat
     9541 ttgcgtcata aagaacacta atcttactgt aaatcctctt tatattaata tcctcttctt
     9601 ccataacgag agtagtcttt gatatgttct gtgaagtagt ctttgtatac gtatatacaa
     9661 atttgtacat acctctacct ttttttaaag agacatatct ctttttttcg atacccctct
     9721 gacgaatttt cggcatatac tccttttttt cgcaatccaa agtaggaaaa gtaatttcta
     9781 tcaatttatg tacatttaaa acaatgtaca taacatttga taatgtaact gctcttttct
     9841 cataataaat atttctaaaa acccttttga taatcccctt tttctctaag tacacaattg
     9901 catcttttat agctcgttct gataatcctg tttttttgct taattgctta tatgattttt
     9961 gcaaaatatc ttcaagaaac tttttttgat aaccaagtag ctgaccatca tcgttaaaaa
    10021 tttttttagg tctataccaa taggtaattt ctgctaagat aactattgca ttcaaatagg
    10081 gcttctcaac accttttttc gttttttttc ttaattgatc gtaccatgtg tgtggattac
    10141 atttccgtca aaatttattt ctctaagttt gtcaactaca acatttccag aagaaaacac
    10201 cttttcacct tcttttttga tataatagct ataaataaag ttttctcagc actttattat
    10261 tgagtgttcc cccagtctgc aaacttttgg gaacactttt tttctttttt taaagcattt
    10321 ttttataatt tctacttcat catcttccaa tattttcccc tcttttcatt cgagcaaaca
    10381 taaactcatt aactgccttt ttcgaataaa attttcttgc ccctaattca actacgggca
    10441 atccctcatt tctcacagca ttttttaaaa agttttcact cacatttaag tattccgcca
    10501 tttctttttg attgagatat tcccttgtca tccccgcatt ttcttgagcc ttttcaatag
    10561 cttttgtagc aatttgataa aactgcttta gaaacatttc ctcctgttct tctgaaatca
    10621 ttaattttat tgcttccaca aaaacctctc ctttttaatt aattattcta taactcctct
    10681 acctttataa gtacaaataa atacaaattg cgtaaaaaaa tgtatttatt tgtatttata
    10741 agaataaagt atcatactcc agataaaaag taaatacttt taaatacttt tattttataa
    10801 atatgatata ctatctttaa aaataaaaag gagaaaaaag atgaagccaa atccttcaga
    10861 agcaggaaaa cgaatcaaac aattacgcct ttcatgtggt tttactatgg aagaacttgg
    10921 aaggaaaata gataattcac cacgtgcgac catatccaac tgggaacgtg ggaccaattt
    10981 accaaatcca caaaagctga aattattatc aactatcact aattcaacaa ttgactggat
    11041 taaatgggga actttagaag aatatataac ttcatacctt atcgatatag ggtatgagct
    11101 atatattaag gacttcccag aaatacctca caaagtcttt aaagacattc aagaaagata
    11161 tagtgatacg ttttcgctag ataaagacta tgagttattg aatcctataa taaaaaatat
    11221 atttacaaaa tattactcaa aagattttga aatttatcta aatgagatac ttgctaataa
    11281 tatcaatcct ctgattgaag agtacgctaa ccaaagacac tctatctcta aagagctatt
    11341 tttcaaaaga gccaacagta aacttcaaga caagataaga aatggtgatt tcaaatacgg
    11401 tgaaaaagaa agaatgattg attctttaaa acagctatta gacgaaatgg acttagccta
    11461 taaagatatt tcaaagtatt catctgtcga ggatttcttt tataaaatta ctaaaaacca
    11521 atttgaaaca gaaaaattta tttcagatct tagtaaaaaa tacaatttcc catatgagaa
    11581 aaatagtata atcgcaaaat ttttaactga taatcacgat aaatttaaaa ataattgatt
    11641 cttcaaataa cttctctacc tgcaataatg aagtaaggag agaatcaaat ggcaacattt
    11701 tttagttata aattacaaaa tggcgaaaaa tattggggat ttgaaactta tttagggaaa
    11761 gatcccaaga ctggaaaaga tatacgtaca aagcgtagaa gattcaaatc aaaagctgag
    11821 gcgcaaacag cacttaatag actacttgta gactttaaag aaaaacaaga gtttaaaatc
    11881 aatgttacaa cttttcaaga gttgtatgaa ttatggattg ataactacag aacaagagta
    11941 aaaccgtctt ctgttgccgt tgctcaacgt ttttgcaaaa accatatatt acctgctttt
    12001 ggggaattac gattggataa aataagtgtt agctattgcc aaaaacaggt gaatgaatgg
    12061 cacaaaacat taaaacaata tggtttcttg agaaaaacaa ctgctcaaat atttaaattt
    12121 ggagtagcaa tggaagtctg taaggataat cctatgtcta aaacattgct tccaagaaaa
    12181 attgaagaag aacaaccctt gaagttttac acgaaagatc aattacaact gtttttacag
    12241 aatactaaag aaaataattc agttaaactg tacacttttt tccgtttatt agcttataca
    12301 ggaatgcgta aaagcgaagc attggcgctt caatgggaag atatagacta tttcaataaa
    12361 acaataacaa ttggtaaaac tatcgctcaa gacgagttta atcaagtagt tctacaagta
    12421 cctaaaacta aaaattcatc aagaacgatt cagcttgatg attttacact taaacaactg
    12481 cgtatttggc aacaagaaca aatgaaaata atgatcttat atggctataa cacgaattca
    12541 ccaaaacagt ttcttttcac tacaaacaca aacaagttgt actaccccca agtagtaaac
    12601 gattggtcag actggattta caaaaagaca ccgatggagc ctcaaattac tccacatgga
    12661 tttaggcata cccactgtag cttattgttt gaaagtggtg cgtcgataaa agaagtacag
    12721 gaacgattag gtcataaaga tattaaaact accatgaata tctatgctca tgtcacacca
    12781 caatctgtta agaaaactgg tgaccgattt gcaaaattta tgggcgaata attccaaacg
    12841 tagccaaaaa cgtagccaac cattaaaata caaataaaaa aacgctcgaa acgctttaat
    12901 atcaacgttt cgagcaatga aatagttatt aacctacaga cccttccatc tcataactga
    12961 tcaatcgatt gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa
    13021 agcccatgac gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt
    13081 agagttgttc ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt
    13141 tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt
    13201 cacattcgat atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat
    13261 agtttacttc tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg
    13321 gtgcattgtg gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga
    13381 tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg
    13441 ttgtttttgc acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg
    13501 ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc
    13561 aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat
    13621 aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca
    13681 gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa
    13741 catcgacttt gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat
    13801 tcagcgctgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata
    13861 attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga
    13921 attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta
    13981 gataagctcg ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg
    14041 gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat
    14101 cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt
    14161 ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct
    14221 cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt
    14281 gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg
    14341 tggtaatgat ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag
    14401 gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca
    14461 tcgcctaatt ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca
    14521 gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca
    14581 cttgccgtac tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct
    14641 tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg
    14701 cttgtcgagt cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact
    14761 tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat
    14821 gattgcttca actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg
    14881 agcagtcgct gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct
    14941 gacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa
    15001 attgaatgca acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc
    15061 atcaattgct tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat
    15121 attgtccata ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc
    15181 gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa
    15241 ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt
    15301 tcgtttacca tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc
    15361 aaagaaatca cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc
    15421 atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt
    15481 gaagacacct aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat
    15541 ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa
    15601 gacagcacct gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat
    15661 gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac
    15721 ccaattcaag cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg
    15781 gatgaacgcc cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa
    15841 cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg
    15901 gagtacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc
    15961 attgacgtct tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca
    16021 ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct
    16081 gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat
    16141 cctctactca tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt
    16201 ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta
    16261 tcagaaagca tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag
    16321 atgtggccga taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa
    16381 atatgcccga tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt
    16441 ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct
    16501 gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa
    16561 gcatctcgta aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga
    16621 tctacggctg aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta
    16681 cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca
    16741 ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct
    16801 tgaatgaata aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa
    16861 ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact
    16921 gcttttttca tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag
    16981 tcggtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat
    17041 gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct
    17101 gtttcactga tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc
    17161 cgatcgatat gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt
    17221 ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg
    17281 agccagtttt tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt
    17341 cctaattctt tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca
    17401 ttccctgtca tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag
    17461 ttcaataatc gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca
    17521 tttactcctt tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct
    17581 aaaattgcaa atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct
    17641 ccgccagaaa aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa
    17701 gccatttttt catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca
    17761 cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt
    17821 ggatattgca tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa
    17881 acatttttcc cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg
    17941 atagcggcag ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct
    18001 ccggttttca ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt
    18061 agatttttta tttctaagac agacatgcat tataccccca aattctcttt cgttctcact
    18121 cattttagac taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt
    18181 tatttattat taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat
    18241 aagccattac agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt
    18301 tactggttta acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg
    18361 aagcacttca atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat
    18421 aatattcacg taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa
    18481 ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta
    18541 cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc
    18601 ttcaatatca tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc
    18661 agcatcttgt agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat
    18721 tgttgcacca tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg
    18781 ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt
    18841 caaataagga atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt
    18901 cggtaataca tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa
    18961 tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt
    19021 cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa
    19081 tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat
    19141 ccccctatat cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga
    19201 acgataaata cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca
    19261 cgctggtagc cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc
    19321 atcgcagtaa atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga
    19381 atactttcag gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct
    19441 gctgcttcca gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa
    19501 aatggtgctg cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct
    19561 acgattgctt tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg
    19621 aaaatattac tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg
    19681 cgtccaatag agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt
    19741 gagattaatg tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt
    19801 tgtttaaaat ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc
    19861 tactccttgt tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat
    19921 caagacagtc aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac
    19981 gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct
    20041 ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa
    20101 tagttctgtc gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc
    20161 ttttttcggt tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc
    20221 catcacagcg actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac
    20281 gatggtcaaa ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt
    20341 ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa
    20401 tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg
    20461 tccgcgtcct tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct
    20521 ctttggtacg ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca
    20581 taacaggttg aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt
    20641 gttttttaat tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc
    20701 aagtccattc aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc
    20761 ataaatatcc cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt
    20821 tttcccattg aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc
    20881 tttctcttat aatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg
    20941 aaagcttgaa cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt
    21001 ccagtacttg gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag
    21061 taagtttgct tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac
    21121 ccattttcag caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag
    21181 ctttctcgtt tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact
    21241 tttttcaagt gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga
    21301 aacttaagat ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga
    21361 ttttatcatt aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt
    21421 tttccgcttc aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca
    21481 gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt
    21541 attcttttcc attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca
    21601 ttttatatca gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc
    21661 tctcgtaaga tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct
    21721 tttgaagccg taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg
    21781 cttgtgttga acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt
    21841 gaggccttcg gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct
    21901 aaccacgctt tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat
    21961 tttcttacct ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca
    22021 acccgatacc gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg
    22081 ggatccctgt taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca
    22141 gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac
    22201 acacccgaat catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga
    22261 taaagccgaa gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat
    22321 agatattgct tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc
    22381 cttgggccgg ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa
    22441 tacgtgcaaa ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag
    22501 tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga
    22561 tgatgcgcca agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa
    22621 gcatcgttcc aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca
    22681 agagcttccc aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat
    22741 gtttagttac gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct
    22801 ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct
    22861 gatcatagaa tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag
    22921 tcagtttcca gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa
    22981 ctaaatacca gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat
    23041 ggctaagtgc tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa
    23101 tcacgccata atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc
    23161 ccttttctta aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc
    23221 tttccataga catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca
    23281 gtacaaaagc ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt
    23341 atctaccagc tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg
    23401 atctcttcgg caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa
    23461 aaatatcgat ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac
    23521 aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg
    23581 atcatcttct tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca
    23641 ccagagtttg cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc
    23701 tggtcttcca tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa
    23761 ccaaaatcgg attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg
    23821 atctcaaaat gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga
    23881 tagacagcaa aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg
    23941 agcaattttt gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat
    24001 aagtcggctt gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata
    24061 ttcaattccc gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt
    24121 acttctgctg ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac
    24181 ttaaacatgg caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt
    24241 aaatatacaa aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta
    24301 gtaacttaga tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt
    24361 ttacaatgta ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata
    24421 tatgaagaac tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc
    24481 ttcccttgga aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg
    24541 tatctctatg attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac
    24601 gaaacaaacg ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact
    24661 catggttatt tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat
    24721 ttggctgtgc gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt
    24781 tatcaaagct gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg
    24841 atttggggtc atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt
    24901 ttggatttca ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt
    24961 cgtaagaaat atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc
    25021 tctttgccta acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc
    25081 attgttcgct ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt
    25141 caagcttcgt ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac
    25201 agctatattt actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga
    25261 tttcttaaaa gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca
    25321 ttacgtacgt ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat
    25381 cgcttaataa tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg
    25441 gccaaacaaa aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa
    25501 gcttttgaac accgtttacc aagaggtcgt aaaagaagtg gtcagcttca agaaataaga
    25561 aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag
    25621 ctacttctgg aacaccgttt attcgaaaca agaggtcgta caagactttt gtcacgacct
    25681 cttgttcttt tgtcaaaata ctggtttagt gctactatgt tcgagtatgc ttttagtatt
    25741 tattgacgaa aggaaccgat caaatgaaat cattgtcaca tccaataaaa tctgtaaagg
    25801 attcagcggt ccgcgaaatc ttacccggtt tactgctttc ttttgccatc gctgccttca
    25861 gcaggcttct tgctatttat ctgccacagt taggtggagc gactattgct attttattgg
    25921 gtattttatt aggaaatact ttttttaaac agcctgttct agcaatcggc acaaaatttg
    25981 ctgaaagcaa actattagaa ttctctgttt ttttactagg tgcaactgtc acagctcaaa
    26041 cgattgctca aattggtgta aaaggaattg tttttgtgtt actgcaaatg tcagtcacta
    26101 tcatcggtgc ttacttgatt ggtaaaaaac tgcaattcag cgatacttct tctttgatga
    26161 tggctggagg aaacgctgtt tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc
    26221 atgcgaaaga ggaagaaaaa ggacagatca tcacactagt caatctactg ggtactgtga
    26281 tgatgttgac tctccctttt cttggattac agttgtttgg tggaaatctc attgaaaaaa
    26341 gtgcactttt gggtggtaca ctccaatctg ttggtcaagt agtcgctggg gcaagcttac
    26401 ttgacaattc tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt atgatgctcg
    26461 ttgttgttgt ctatctgttt gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa
    26521 cgccaactga tacgcagaaa aaacgaaaat taccgcttcc ttggtatgta ttagggtttg
    26581 tgatcttttg tttggtcaac agcttgtttt ctttacctgt agttattgac caatcggcac
    26641 attttatcag cagctggttt gagatcactg ctttagcggc aattggtctg cgtttagact
    26701 ttgcaaaatt catgaaagaa ggtacacgct tcttatctta tggattagct gtcgggcttc
    26761 tccaaacagt gatggcttta ctatttattt ggctgatcaa agtttagaat gaacatgaaa
    26821 aaacagttgt gattctcgca tggaacaatg tccgaatcac aactgttttt aattttgcag
    26881 atatccttct aacaacaaca attggcgttt gatttcaacc ccatctattg cgtttcccat
    26941 atatcctgtc agttttccat tttgcccgat cactcgatga caaggaataa cgattggcaa
    27001 tggattggtt cgatttgcct gcccaatcgc tcgcacagct ttcggactat caacagcaac
    27061 ggctacttct ttatacgttc ggatttctcc ataaggaatg gtcgacaaag ctttccagac
    27121 attttgctga aaaggcgtac ctctctttat agataaagga acatcaaatt cttttctttt
    27181 tcccgcaaaa tattcttcaa actgttcttg tgtgcgcgtt aaaattcgct gattcgctga
    27241 ttcgctttct gaaattggct gaaaagatac agcagtcaat ccggattcat ctgcatctaa
    27301 ccataaaaag ccgataggtg tgtgtatttt cattttgttt gctcctagtc aatagactgg
    27361 aacttcctcc tttttctcta tccaaaacta cttgccaact aataaaaatt gttcttaaaa
    27421 gtttgataaa ccgaggaaac atggtaaact aaatacgtta gtttacaaaa agaatacctg
    27481 ttcttttacg taaaatcaaa ttttaaaggt gttatttaag gtttattaaa ttttgaatgt
    27541 aaaccatata taaacatacg attgaaagga tagaaacgat gaaagtatca attgttgtcc
    27601 catgttttaa tgaagaaaaa actgttcctc ttttttttga tgaggttgaa aaattcaaag
    27661 gtgcccatga gttcgaatat ttgtttattg acgacggatc aaaagacggt acgttagcag
    27721 caatcaagaa gctagcctca gcttatcctt atgtccgctt tgtctcattt tccagaaatt
    27781 ttgggaaaga agcggctctg tacgctggat tgcaagcttc tacaggagat ttagtgaccg
    27841 tgatggatgt tgatctgcaa gatccgcccg aactgcttcc tgagatgatc aaccggatca
    27901 ctacttctga tatcgattgt gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga
    27961 tacgcagttt ctttgcgaaa aaattctacc agttgatcaa taaaatcagc gatacagaaa
    28021 tggtcgatgg cgctcgtgat ttccgcttga tgactcgtca aatggtggat gccgtcctag
    28081 aattaacaga atacaaccga ttttcaaaag gattatttag ctgggtcgga tttaaaacag
    28141 aatatatttc ttatgaaaac cgcgaacgtg tggctggcga aacatcttgg tccttctgga
    28201 aattattcaa ctactcgatt gatggtatcg tgaacttctc ggatgcaccg ttaacgattg
    28261 cttcatttat cggcgctttg tcttgtgtcg gttctggtat tgcaattttg ttcattattt
    28321 tgcgggcatt gttgtttggt gatcctacat caggatggcc ttccatggtc tcgatcttcc
    28381 tgttcattgg tggaatccaa ttgttatgtc ttggaatcat cgggaaatat attgggaaaa
    28441 tcttcttaga aacgaaacag cgtcctgttt atatcataag agaaacagaa aaagtgaagc
    28501 caaatgaatt gaagcctcac caataatata cgtcaaatga agtaaacatt tttttaaatg
    28561 aaaaaggact gtgacaaaag tcttgtcaca gtcctttttt ccgaataaat ggtgttccag
    28621 aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    28681 attccttctt atttcttgaa gctgaccact tctatcacaa cctctttcat atcttattac
    28741 ttcttcattg ctgcttttag tgctgctgct aatgggcttt cttctggttc ttctgcttgt
    28801 gcatattttt tcagcaagcg tcgttcttca tgtttggtca ttttcttctt ctgctctttt
    28861 ttatcaagca atttttctgt cgttccatcg tatttacaac ggaaataagc accgttttta
    28921 ccttcgatga tttccatttt ccggtgacat tgcgggcatc gatgattaga gacttttgga
    28981 tcttttcttc tgcggtagtt gcaatctgaa ttggtgcaga catagatttt gccgtcacgt
    29041 gtgtttttct cgcgcagatt ggccccacat tctgggcatt tcttttgggt aatcgagaaa
    29101 tcttgatatt cttgtttgct tgatttgatc tcattcacca agcgttttgt ttctttttcg
    29161 attccattca agaaggttcc tgcttgtttc tttcctgcag caatagcttc aaggtctttt
    29221 tcccagcttt ctgtcaaatc tggtgttact aaagagggat ttaccaatac taataattgc
    29281 ttacctttag gagatacttg cagtccttga ggggtacgtt ccatcagttc agatttgatt
    29341 agtttctcga taatctctgc acgcgtagct ggggttccca gactgtattt ttccatttga
    29401 ccaagcaagc ttgcttctga caatggttta ggcggtgcag atagttcttt tttgatggcg
    29461 aaatcgggat tgattctcat tcctttttcc catttgactg tttccgtctc agtttcagtt
    29521 tcgcctttcc atcctgcttg gacgacacga ttttgccgaa aaatgaatcg ttctttacca
    29581 aattcaaccg taacttttgt ttggctgact ttatggggtt gagcaaacag tcctaaaaaa
    29641 cgagagacaa tcatttgata gatttttcgc tcttcattac ttaatttttc ataacgtgct
    29701 ctgttttcag ttggcaaaag tgcatggtga tcggtcactt tactgtcttg gaatactttt
    29761 gtctgttgga cgatggctcc gtttttcaaa tactgcttga cctcaggagc aaagtcgctg
    29821 actgcttgca atcgttcttt catcgtactt ttcatgtcat ttgtcagata tttgctgtct
    29881 gtacgcggat aagtaacgat cttatgggtc tcatacaaac tttgtacaag acttaatgtt
    29941 ttctttgcag aatagccata gcgttgattg gcttctcttt ggatctctgt taaatcataa
    30001 ggcaatggcg cagattccgt ttttaatttt tcttggactt ctgtgacagt tcctttgctt
    30061 gtactgatca attgaacaag tttttgtgct tcttcttgtg tttttaaagc aaattgattt
    30121 ttttgaagca ttttggcttg ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt
    30181 ggacggaact gctcgatttt cttttcttgg tctctcacta atgctagtgt cggtgtctgc
    30241 acacggcctg ctgataaatt atccgcgtac ttcacagtta aggctctggt cacattcaat
    30301 cctaccagcc agtccgcttt tgcccgggca agagcagatt catacaatgc atcatattct
    30361 ttcgcaggtc ttagttgctt gaatccagtt ttgattgctt tatctgtttg agaagagatc
    30421 cataaccgct tcactggttt attgaaatgc acatactcta agatccagcg agcaacaagt
    30481 tccccttctc ttccggcatc tgtcgcaatg actgcttctg agacatcttt tcgctgagcc
    30541 agctgcttga ttgcttttaa ttgatgtcct gttttaggaa gtggtttgat tcctagtttt
    30601 ttagggatca ttggcaacgt gtccatttgc cagctctgcc attccttatt cagatcttcc
    30661 ggcattttca atcctaataa atgaccaagg gcccaagtaa cgatcacctt gggtccttca
    30721 taataatttt tgtgcttttg attagcacca agcaccttgc ttaggtcttt cgcaacgctt
    30781 ggtttttccg ctagaattag ttgtttcata aaattccttt ctgctttcta ctgtgatggg
    30841 ctgatgattt ttcaacaacg caagtccctt gtattgctgt aaatcatcgt cacactcttc
    30901 acaccagcgc tcatctccgt cattccaaaa ggcgctgaga aagttacttt ttgcattgat
    30961 atattcataa ttttgatgga tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc
    31021 gaaagactcg aatgtttctt cttcttcaat atcttcttcc cagtcttcaa aaaaccacca
    31081 aggctcattg tccccgaaca tggtaattac ttgatacata ctcgtgctcc ttctttcagc
    31141 ttttctagac tcatcttagt cacttttcaa gaaagttgca agaattttct cttataaaag
    31201 ggtcatttaa tgaacatctt aaaattgtta caaatgaatc tgattgttta aggcaaagta
    31261 gcgataaagt tattgatcat ccaaaaatag ctgtcttctg gatcgattgt tggttctttt
    31321 gtaaagtatc ctaaaaaacg taaggaaaga taaccgtgga tcatactacg aagttccctg
    31381 cttcgatgaa ctttttcttc tttcgttaat tgaaaagcct caaatatctg caaaatgatc
    31441 tgattcgttt catggattcc atctaataaa acagcttcat tggtatgcgg tacactgatc
    31501 agtaactcat acactgcttg atttgaaaaa gcaaaggatc gataagtttc tgcatagatc
    31561 tgcagtgctt cctgaccact ttttcccaca agagcccgtc gcagctggtc attaagttcc
    31621 tgcagaagtt tcgctgttag cgctgttttg acatcacgaa tatttttgaa atggttatac
    31681 agcgagggag ccttgatatc caagcgtttg gctaaatgct gaaaggttat ttgctgaaca
    31741 tccatctctt ctgctagttc tcgaaagcag tcgatgattt tctcttgaga cagatttctt
    31801 tttttcattt ccatgtttcc tctcataaaa taaatccttc actctttcag tttaccacga
    31861 aaaagaaaga gaaaaaagag actttcattg ataaactaca tattgtagtt tataataaag
    31921 agctgtagat gaaaattttc agaaagaagg agtctaattg gctgcaagaa taaaggaaaa
    31981 attatttagg aaaagagaaa cttgggaaca aaacttgttt gtcttatggt ttggaacatt
    32041 tatggctggt atcggattta gtttagtcat gccttttatg tcactttaca tagatacgtt
    32101 aggtaattat agtacgtcac aattgaactt ttggagcgga ttgacttttt catctacttt
    32161 cttggtgact acgcttatct ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa
    32221 attgatgctg ttgcgtgctt cattaggaat ggcaatcgtc atttctttga tgggttgcgt
    32281 gactagtgtg tatcaattag tcgcattgcg tctgcttcaa gggatttttt ctggttatat
    32341 cagtaatgcc actgcccttg tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct
    32401 tggaacctta gcaactggtt ctgttacagg aacattactt ggaccgcttc ttggcgggat
    32461 ctctgcttca gcgtttggct atcgccctac ctttttcatt acagggatca tcttattttt
    32521 cgtcttcttg ttgagtctgt ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa
    32581 catggtttct gccaaacaga ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat
    32641 gttcgtcaca acgatgatca tccaagcatc caacaattcc attagtccaa tcatcagtct
    32701 gtacattcgc caattgctgc atggacatgg gaatgttacg ttgatcagcg gaatcattgc
    32761 ttcgatacct gggattgcca ctttgatagc cgctccaaga tttggtcgct taggtgacaa
    32821 gattgggagt gaaagaatac ttgcgattgg tttgggtttt gcgattctgg tttatatccc
    32881 aatggcattc gtcacgaatg tttggcaatt agctgcctta cgttttttga ttggtatctc
    32941 tgatgcttgt ctgctccctg ctgtccaagc attgatcaca aaatattcgc ctcatcaagc
    33001 tgctggacgg atcttcagtt ataaccaatc tttccaagca actggtaacg tagtagggcc
    33061 tatgatcggc tccagtgttt ccagtgcatt tggttatcgc ggcgtctttc tttcgacttc
    33121 tcttcttgtt ttggtcaact tcttgcttgt acgacacaat acacaagaga tccatcagca
    33181 agaaactgca gaagaaaaag cttcaggagt ctccatagct tcagggcact ctccttctca
    33241 tagacaataa gcgtaaatga aaaacactgc ttgaacagtt gtcacagact gtccttgcag
    33301 tgtttttgtt taggcctgtt ttttcaagaa ctcagataag aggaataatc ccttttccaa
    33361 ggtttctttt ttacaacagt aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc
    33421 tggtactaat agtactcctg tttcttctaa taaattgata caaaatgtct ggtcatcttc
    33481 ttcgatggct aatttaatga aagaagtaga gacgtaattg ggagcgacaa gttcaacttt
    33541 cggttcattg gctacccatt gttttaaaat cacaaggttc tctgtaatga tctttttgtt
    33601 tcgcgccaat attttttctt tattcttcaa tgcatgtaca gcaagatcat ctgacaggac
    33661 tccgccgcaa atcatcgtgt agtctcgata ctttcgaaaa acttctgcca actcaggtcc
    33721 agtagctgtc cagccaatac gaatacctgg aatggagtag gttttggata gggaattggt
    33781 tgctatacct ttttcataga catctacgat cgacaagaat tcacctttat ccgttaaagg
    33841 cgcatatact tcatctatca atacatatgc atctactgta cgagcaattt ctgcgatttt
    33901 ttctaaagtt ttccgatcta gcaatgtgcc tgtaggattg tttgcactat tcaaacagat
    33961 gatttttgtt tcaggtttga ctaaggcttc tagctgttct aaatcaaact gccaatcttc
    34021 ttcttccttc aaatgaacaa agtcaacagc ggcacctaaa gatttgggga tgtcatataa
    34081 ttgctgataa gtaggaagca tcgaaacaac atggtctcct gcttccacta acgcgtatag
    34141 agctaaaaga ttagcaccag tcgctccatt cgtttgtaag atgttttccg gatcgacggt
    34201 ttgataaaga gaagcgactt cttgtttgaa aacatctgat ccttcgatcc agccatagtc
    34261 caatggttcg tttttgcgtt tttcgaaaaa tttacttacg ctagtcccat ctaagccaac
    34321 gagttcgtct aatgtcaatg cttcgatcga gctttgtgca atatcatata ccgctttctt
    34381 ttcccattta tttagccatt cttctacacc gaaatttgcg attttcatgt ttctttcccc
    34441 cttatatctt gatccagcct acagcaagca acccgattga aagcagtgcg gccacacaaa
    34501 tcagcccaat cattactttt tcttttcccg taatttgatg tttggtttca cgacaagcga
    34561 tccagtagaa gcaaaaacca ggaagatagg caatactaca taaaaggaca taagaaaatc
    34621 ctgctaaaat gatgcccatc aattcaaaaa ctacagcaat cccaccgatc actacttgtt
    34681 ttttgattcc ttcttttaaa gaaaactgga cttgatagat cccgacaagt agataacaaa
    34741 tcaaaattgc cgctgtacat aaagaatagg caaattgata ggcataatca gtaactaaga
    34801 aagtcaataa gaatagatta gtcaagattg cagtaacaac taacgaataa gttggggcat
    34861 tttttttatt cgtttttccc caacctttcg gcaacagacc gtctcgagcc atcaacatcg
    34921 ttgtttctgc tgggagcatc gtccaagaca accaagaacc aatgatcgaa ataatcaagc
    34981 cgatattgat cagcgctgct ccccaatttc ccacgattcc ttttaaaata tttgccatag
    35041 caggttgtga aatagttgct aactcttctt gagataagac gccgtatggc aagatcgaag
    35101 caaggatata gataaacaat aaacaaagca gaccaatgat cgatgcttgc tgggcttctg
    35161 aacgtttctt cgcgcggttg gctaaaacac ttgcgccttc gatccctaca aagacccaca
    35221 tcataaccat caaacaacct ttaatctgca accagatcgt tccttgctgc cctgttccat
    35281 tcactagatt atcagatacg ttaccccaaa aatcagcagt gaatatattc actttaaatg
    35341 cgataaatac aaatattaaa aataggaata atggcactaa tttacagatc gtcacgattg
    35401 tattgatgac gctcgctcct tcaactccat tgtttactaa taaagtcaga ccccaaataa
    35461 aaatacttgc aaataaaata gaaggaacat tttgtccacc tttgaatatc gggaaaaatt
    35521 ctcccatggc gctcatcagc atcgttgcaa aagcaacatt tcccagccac gcagacaacc
    35581 agtaacccca accactgatg aagccaccta atttaccaaa tgaagcttcc gcgtaaccga
    35641 aaataccacc atctagatct ggacgctttt ctcctaaatg attcagcgat aataccaaag
    35701 ctaaaatacc aatcccaacg attatccatg aaaggatcgc tggccctgga gctgcagaac
    35761 tagccagatc acttgtgatc ccaaacacac cgcttccgat tgacgaactg acaaccagag
    35821 caaccagtcc ccatttgcta atacccgtcg atttcttttc catttccatc accctcactt
    35881 ataaaattga acaaatcact tgcaaaagat tcccttctat tttgcaactg ctcttgctta
    35941 acaaaaagca actttattca tattattgga tattatgaac tttttacaca agtatttcaa
    36001 gtataaaatg aataaaaatt aattttattg caaaaaaaca gctatgctat ccaaaagaaa
    36061 aggtgtttct tttgttagcc atagctgttt tgattcattt aatatcgctt aaacgtattg
    36121 taaaactttt gtttcttgat atgcgtggtc gatcaaacca ccacccagac attccattcc
    36181 atcataaaat acaactgctt gtccaggagt gattgcacgt acaggttcgt cgaatacgac
    36241 ttctgcacga ttatcatcca ataagcgaac tgtcacagga acgtcttgct gacgataacg
    36301 gaatttcgct gtacacttga attcttttgg cattggttca ttcgtcgtaa agtggatttc
    36361 acttgcatcc aagcttgtgg catacaatgc aggatgatgg aacccttgcc ccacatataa
    36421 tgtgtttgtc gctagatctt ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc
    36481 accgatccct agtccttgac gttgaccaat cgtataatac atcaagccag catgttgtcc
    36541 tttgacttca ccatcaagag tgaccatatt tccttttttt gctggcagat agttgctcaa
    36601 aaattgtttg aagttctttt caccaataaa gcagattccc gttgaatctt ttttcttagc
    36661 agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct gatttttcca tgccgcctag
    36721 agggaacatc gtttttgaaa gctgttcttg tgataattgg ctaaggaagt aagtttgatc
    36781 tttattattg tctactccac gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg
    36841 cgcataatgt ccagtagcta cataatcggc gccaagatcc attgcataat ccaagaatgc
    36901 tttgaatttg atttctttgt tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta
    36961 ttcggctagg aaatattcaa atacacgatc ccaatattct ttttcaaaat tcacagagta
    37021 ataaggaatc ccgatttgag cagctacttt cgctacatcc ttataatctt ccgtggccgt
    37081 gcatacaccg ttttcgtccg tgtcatccca atttttcatg aatattccga tcacgtcgta
    37141 tccttgttcc tttaacaaaa gtgctgttac agatgagtcg acaccgccgc tcatccctac
    37201 gacaacgcgt gttttgctgt tgtctgtcat gatatcacca tcatttcaat tagattctga
    37261 aacatctacg agttgaaggt cgcaactttt cagtatttta cattatacgt catatttctg
    37321 aaaatttcat cccttaatta tcaagtcgta aaaatactgt tctactggac ctattcagta
    37381 cacaaacatg atagtcaacc aaaaaatcac tcatcttttg agcattcgat ataaaaaatt
    37441 ggcatactca taaaaagggc cagcctttcg gcaataagga ataattttat aaattgtgaa
    37501 aaactatttt tcaaaattgc ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt
    37561 tctttttaga tgttatgagt tgctcagata agtgattttg tttactctaa gaaaaaaggg
    37621 aggcaaaaga tacatgaatt actttatcag tgatatgcac tttttccatg aacgcttatt
    37681 aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg gatgaaatga accaacagtt
    37741 gatcactagc tggaatgcag taataaaaga aaccgaccat gtttatcatc taggcgacct
    37801 tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact
    37861 gcaaggaaca atccacttga tcaaagggaa ccatgacagt cgcgcttttt tccgttattt
    37921 agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa ttttattttt atgatgtagg
    37981 tgccatcgtt aaattcgacc atcaacaata ttacttgact cattatccga tgttgctcgg
    38041 gcctaacgac actatccgca atcttcatgg acatatccat cattacagcg tacctatcga
    38101 gaacgatgtc aatgtaggcg tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc
    38161 atttggcact ccgttatctg agcaacaaat caaagaaatc tatgaagcaa agaggaagta
    38221 cgataggtcg tgaaaaaact gccctatatc aagtattaaa caaataaaga taattactca
    38281 tatttttcat ttgagtaggc ttattgccac aggaacatta ggatcagaaa tggagttgta
    38341 acaagacttc tgtcacaact ccattccata ctattcgtct ttttgctcat ggtgttcttt
    38401 taccagtttt ttctgtaatt ctttatcata atttcgggca gctgacgtta aaaataaaac
    38461 gattccgcat aataaaatcc cgattccgga aaacaagaac attttttcta cccctaaacg
    38521 atcagctaat ggtcctgcaa ataataatcc aattggacct gccaggttca tcaaagagtt
    38581 gaagttccct aagacacgac ctaaaacatt cgattcataa ctctgctgga tcattgccat
    38641 cagcagtgta ttgaaatatg gcgttgcaca acctgctcct gcattcaaga taagaaagta
    38701 caagaaccct tgactatcgt ttggaaccaa tccgcttgca ccgatcgtca ggcccataat
    38761 gatgtaggct ataataatag gtttcattcg gtctttccat gttcctataa aactgatcag
    38821 tgcaccgcct aagagcattc cgactgcgta gactacttcc actgctcccg cctgtccaac
    38881 ggaaccacca aagtaatcta gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa
    38941 cagcatcaca aatgcaccat tgatcgttat gtaccataac cctttattat ccatcaattg
    39001 ctgcagccca aattttgcat ctttaaaaat agtaagcgga acttccaata tttcaggaga
    39061 aacttttggg atcttcacaa aaatcaataa gccgacacca aaaactgccc ccaatacatc
    39121 taataaaata aggtaattga caggaacgac tgaaaacaaa gccgcaccta gagctggcgc
    39181 aatgatgaaa ttcgctgatt ggaccattcc taactgtcca ttcgtccttg ttatgtgaga
    39241 agatggaacg attgtcggca taatcgactg gatcgttggc atttggaatg tttgtgcgac
    39301 cgaacgcata aacaaagaca caaaaactag ccaaatcggg aaggattctg aaatcgttcc
    39361 gactactgct aaaataagcg cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc
    39421 ttttttatcc cagcgatcca ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat
    39481 cattcctagt aaagtagcaa aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata
    39541 ccagatgatc gcatattgga cgaccatact agtgattcct gacaaaaact gtcccgataa
    39601 aaagaacaaa taatttctct tccaattcgt taaaacgacc gactgttcat tttccataaa
    39661 catccctctt ttctttttca ttttgtatag cgtttgcaca acaatcatta tacgggattc
    39721 ttttttcaaa aaataccttt ttccctaaat aacaatcgtt tcaatttaaa tttctttact
    39781 tagaaacaat aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa
    39841 aaagacaact aatttgcgtc gtccttacaa
//
LOCUS       DASHWH010000020        39615 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.020, whole genome shotgun sequence.
ACCESSION   DASHWH010000020 DASHWH010000000
VERSION     DASHWH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.020"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..207
                     /locus_tag="U2R41_001081"
     CDS             <1..207
                     /locus_tag="U2R41_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEM8888507.1"
                     /translation="LYAYPQGTVASWITRQRRIKSLPASFVYDLSLASSMNMTDVYEK
                     LLILENEYDQFNSNQQRKVKKQID"
     gene            433..1251
                     /locus_tag="U2R41_001082"
     CDS             433..1251
                     /locus_tag="U2R41_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8888508.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1270..1677
                     /locus_tag="U2R41_001083"
     CDS             1270..1677
                     /locus_tag="U2R41_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888509.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1997..3082
                     /locus_tag="U2R41_001084"
     CDS             1997..3082
                     /locus_tag="U2R41_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8888510.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            3919..4251
                     /locus_tag="U2R41_001085"
     CDS             3919..4251
                     /locus_tag="U2R41_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM8888511.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4842..5171
                     /locus_tag="U2R41_001086"
     CDS             4842..5171
                     /locus_tag="U2R41_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888512.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            5158..5499
                     /locus_tag="U2R41_001087"
     CDS             5158..5499
                     /locus_tag="U2R41_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEM8888513.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5613..5852
                     /locus_tag="U2R41_001088"
     CDS             5613..5852
                     /locus_tag="U2R41_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888514.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5919..6158
                     /locus_tag="U2R41_001089"
     CDS             5919..6158
                     /locus_tag="U2R41_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888515.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            6285..6974
                     /locus_tag="U2R41_001090"
     CDS             6285..6974
                     /locus_tag="U2R41_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEM8888516.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            6980..8146
                     /locus_tag="U2R41_001091"
     CDS             6980..8146
                     /locus_tag="U2R41_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEM8888517.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            8228..8899
                     /locus_tag="U2R41_001092"
     CDS             8228..8899
                     /locus_tag="U2R41_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM8888518.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8952..10928
                     /locus_tag="U2R41_001093"
     CDS             8952..10928
                     /locus_tag="U2R41_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8888519.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            10941..11693
                     /locus_tag="U2R41_001094"
     CDS             10941..11693
                     /locus_tag="U2R41_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8888520.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11709..12470
                     /locus_tag="U2R41_001095"
     CDS             11709..12470
                     /locus_tag="U2R41_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888521.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            12483..12743
                     /locus_tag="U2R41_001096"
     CDS             12483..12743
                     /locus_tag="U2R41_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888522.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12740..14830
                     /locus_tag="U2R41_001097"
     CDS             12740..14830
                     /locus_tag="U2R41_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8888523.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(14866..16923)
                     /locus_tag="U2R41_001098"
     CDS             complement(14866..16923)
                     /locus_tag="U2R41_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEM8888524.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(17068..17259)
                     /locus_tag="U2R41_001099"
     CDS             complement(17068..17259)
                     /locus_tag="U2R41_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888525.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            17447..17902
                     /locus_tag="U2R41_001100"
     CDS             17447..17902
                     /locus_tag="U2R41_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888526.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            17899..18141
                     /locus_tag="U2R41_001101"
     CDS             17899..18141
                     /locus_tag="U2R41_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEM8888527.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18159..18461
                     /locus_tag="U2R41_001102"
     CDS             18159..18461
                     /locus_tag="U2R41_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888528.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            18530..20584
                     /locus_tag="U2R41_001103"
     CDS             18530..20584
                     /locus_tag="U2R41_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888529.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            20584..23196
                     /locus_tag="U2R41_001104"
     CDS             20584..23196
                     /locus_tag="U2R41_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM8888530.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            23193..25571
                     /locus_tag="U2R41_001105"
     CDS             23193..25571
                     /locus_tag="U2R41_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888531.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            25632..26174
                     /locus_tag="U2R41_001106"
     CDS             25632..26174
                     /locus_tag="U2R41_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888532.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            26252..26584
                     /locus_tag="U2R41_001107"
     CDS             26252..26584
                     /locus_tag="U2R41_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888533.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26585..27178
                     /locus_tag="U2R41_001108"
     CDS             26585..27178
                     /locus_tag="U2R41_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888534.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27200..28894
                     /locus_tag="U2R41_001109"
     CDS             27200..28894
                     /locus_tag="U2R41_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEM8888535.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            29582..31459
                     /gene="ltrA"
                     /locus_tag="U2R41_001110"
     CDS             29582..31459
                     /gene="ltrA"
                     /locus_tag="U2R41_001110"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8888536.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            31590..31967
                     /locus_tag="U2R41_001111"
                     /pseudo
     CDS             31590..31967
                     /locus_tag="U2R41_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            31967..33166
                     /locus_tag="U2R41_001112"
     CDS             31967..33166
                     /locus_tag="U2R41_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM8888537.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            33182..33826
                     /locus_tag="U2R41_001113"
     CDS             33182..33826
                     /locus_tag="U2R41_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888538.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            33837..34643
                     /locus_tag="U2R41_001114"
     CDS             33837..34643
                     /locus_tag="U2R41_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888539.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            34666..34895
                     /locus_tag="U2R41_001115"
                     /pseudo
     CDS             34666..34895
                     /locus_tag="U2R41_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34916..35989
                     /locus_tag="U2R41_001116"
     CDS             34916..35989
                     /locus_tag="U2R41_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEM8888540.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            36033..36302
                     /locus_tag="U2R41_001117"
     CDS             36033..36302
                     /locus_tag="U2R41_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888541.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            36578..36853
                     /locus_tag="U2R41_001118"
     CDS             36578..36853
                     /locus_tag="U2R41_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888542.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            36895..37317
                     /locus_tag="U2R41_001119"
     CDS             36895..37317
                     /locus_tag="U2R41_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888543.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            37337..38929
                     /locus_tag="U2R41_001120"
     CDS             37337..38929
                     /locus_tag="U2R41_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888544.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            38950..39285
                     /locus_tag="U2R41_001121"
     CDS             38950..39285
                     /locus_tag="U2R41_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888545.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            39431..>39615
                     /locus_tag="U2R41_001122"
     CDS             39431..>39615
                     /locus_tag="U2R41_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888546.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        14443 a         6053 c         7968 g        11151 t
ORIGIN      
        1 ttatatgcct acccacaagg aacggttgca agctggataa cccgacaacg tagaataaaa
       61 tcattacctg cttcatttgt atatgatcta agtcttgctt ctagtatgaa tatgaccgat
      121 gtatacgaaa agttattaat actagaaaat gaatatgatc aattcaattc aaatcagcag
      181 cgaaaagtaa agaaacagat agactagaat agctattttc gaaaagcaat atttaaggta
      241 taagttatac cttaaataat aaaagtataa gttataccat aaatatatat ggtataactt
      301 atacttttat tattaaaaac cgctatatcc aaacgttttt agatattatt atttaagtta
      361 tacttttaat gttgaccatt tccgatatat aagttatact ttaattatca attaaagatt
      421 gggaggattt acatggatac ttttcaaggg actacattta cagttggcaa ttttaaagga
      481 ggagtaggta aaactaagat agtcagcatg cttgcttatg acaatgcaat gataagaaat
      541 agaaaaacgc ttattattga tttagaccct caagcgaact taacacaagt catagcaaga
      601 actttcggta taaccgatat caaaaccact ataacaaatg gtgtcgcaaa tggagaatta
      661 gcaagtagta ttattaaggt aaatgaaaat cttgaccttc tggcatgtga cactagtttt
      721 cgatcattct ccgcttttgc taataaacat gaaaatgaat tagaccaagt tactgtcttg
      781 aaaaaattac ttcttccaat aaagaacaat tatcaagaaa tatttattga tgtaccacca
      841 accattagcg aattttccga taatgctatg gcagcttcag actattcgat aatttcattt
      901 caaacagctg aagaatcctt agagggagtc aacaaatatg taaattatca aaaattcatg
      961 gtcgatagat acgatcttga tttgcaaatt atagatattg taccttgtat ggtagaacca
     1021 aatgatgatt ttgatgaaga aatcctagaa gaagcaattg aaaaatacgg aaccgtggta
     1081 tccaaaaatc tcattcatta tcaaaaaaga ttgagaaggt attctaaaat tggtatacat
     1141 ttaagaaaat acaaaaatgg aaattttgat caatgggatt ataaagcaca ccaagtattt
     1201 ataaatattt tggcagaact agatgctaga caagagtttt taaataaata ataaggagtt
     1261 gcataagtaa tgcctaaaga ttttgatatt cctaaaatga tggagaatgg tgaatttgag
     1321 ccattattac ccaataacaa acgccaagat gtgaaaataa gcggtgaata taactttagc
     1381 aatagtgcat tgagtaacac aagaaatatt acattgaaaa aaagaggccg accagtttca
     1441 attcctgata aaagaaacaa ggtatcagtt cctaaaaaga tctctccggc attaaatagc
     1501 aaactctcaa ttttacaaga ctatatgacg gagttacaaa gtgaaaaagg gagaatttct
     1561 ttcgaaaaaa ttgttaatac attagcagac tcatatatcc agcatagact aggagtagca
     1621 aaagaagaac acataagaga agaaatacaa gaagagtttg aaaaattacc aaaataattt
     1681 tttttcatgc ggtattcaat taaagtataa gttatactag ataaatgaat attaaagtat
     1741 aagttataca ttaaatattt aaggtataac ttaataaaca acgtgtaaat ttgcgattaa
     1801 atcaacatta taaaattatt atttaaagta taagttatac attatttgta tttaaagtat
     1861 aacttatact tattttttta atatataact tataccttaa atactgaaag tgttttattc
     1921 acccttaata catggtatag ttcacttgtt gaaaatcctg aatatataca agtttctaag
     1981 aagaaggaga aatttaatgt ctacagaaaa tcaatctcaa aaaatgataa ttaaaaattg
     2041 gggatataaa gcaacgaatg tttatattga aaaatattat aaatttccta gttttttaat
     2101 tcagaattct aaatactcta aaatgaaaga cagctcaaaa atagcctata tgctttttaa
     2161 aaatgaattt cgtagagcct tacatgaaaa atggatcgac gatgatggtc tattatattt
     2221 ggaatttaca caagcagacc taatggacaa attaaattgc taccaaggca aagtaaaaac
     2281 cattttaaac gagttagaaa catataattt gatttcagtc gtaaaaggag aatttaaccc
     2341 aaaaacgaaa aaaaatgaaa aaaataaata ctatttactt caacctgaaa taagtgccga
     2401 tgatcttttt attcaaaatt ctttcgatga cgatttaagc gaaactattg ataaatcaac
     2461 ctcttctcgg aatgcggaaa tcgcacgtcg agaggaaact gctaaaaccc ttgataacac
     2521 tggaaatgcg gaaatcgcac gtcgagaaaa aacagctgaa acccttgata acactggaaa
     2581 tgcggaaatc gcacaagatt attatattaa taaatcttta gacactaaaa gacacttaaa
     2641 agacactgaa acagaccaat tacaaaatca agttttgcta gataacttcg ttgaaatcat
     2701 gaaagaagac agcataaaca catttgtacc agaaaaggta ttaagcttga taaaaacatt
     2761 ttctaacact tacgaagaag cccaacaaac cgtcaccaca atacacaaag caaaacataa
     2821 agccgaggaa cttgagaaga cggtagttgt gtatgaagaa cttgaatctt acggaattga
     2881 tgcggataaa ggattatata tgacgttgct gaaagcttat caaaaacaaa agactgagaa
     2941 agtaaataat ttgcagaatt tgatttttgt gtatgtaaaa aactggttta ttgaaaaagc
     3001 tattgcggca aagcttgcaa acgaacaaag agaaacattt aacgaattac caacagttag
     3061 tacggaaaat tggctagagt agttgataaa aagcgaacac tataaaaatg atcaatttta
     3121 aattagaact tttttacaat tttaatttta ttacaaatgt tacaaataaa tagttattat
     3181 tccggagttt ttagattaaa agtttatgaa aacaatcaca aacgtaggtt ttaatgtcat
     3241 aatcgttaga tattttagtt atttttcatt caaaaaaaca gggaactagt gtttgcatat
     3301 gtattttttt gagttataat aattcatgaa gagaggtaat tcacttattt acccaagctg
     3361 aaaagcctga tattacttct tttcaggcag tgctgctaat attcaattac acttttttgt
     3421 ataaatttag ttgttttaaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt
     3481 ctgcactttt ggacgataaa tttggacaat tgaaaataca aaaaaaagaa aggccctgtg
     3541 agactcggaa ggctaccaac cttcgtcgac gtccacaggt ggcattaagt ctatacccct
     3601 taatgcgttt acctttcagt gttacggtat tacccgtaac ggatttagta taacagatat
     3661 tcaaaagtga taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg
     3721 aatctatgcc taagcataat gttataaaaa tgtccgtggg gctctttgcc gtatgacgaa
     3781 cgctgaaatt agagggaacg catgtttgaa tgcgttccct cttttttgtt tgtcatgaac
     3841 gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa tatttaaagt
     3901 aagtgggtgg attattttat gtcatataca ggaattttga gtctcgaaga tatttgtcac
     3961 tacggtaaac ggtgtaccgc tacagaaaag atcacgaaga aactatcaac tggtcaaagt
     4021 aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaagaaatg
     4081 atttattaca ttgggaaaca aaaacaaata attttaaaag atcctatccc tttaaaagag
     4141 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa
     4201 aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt taatcagctg aattgatcat
     4261 tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa
     4321 ggaacttaga atcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg
     4381 ggagtgacgg ggtaggtttt tacgattggt aacgtaaaaa attggtcaat cgagtaaatg
     4441 cccttgtttg gatagtggtg aaatgaattt gtgtaaataa tatgcacaga gtaaacaaga
     4501 gggaatttag aagttagaca agttagaata gcgcctagat taatcatgag cagctactaa
     4561 gggatctagg ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag
     4621 gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga ggacgttgat
     4681 cattggatca atcgagcatt taccgctgtc tactagggtt tctcgtttcg gacgccataa
     4741 acgagtcgat ttattttata tatttttgag tgaacgaaaa acgataaaga tattcgttat
     4801 gaagtaaaac taatttagta gatagcaaag gtggtagagg aatgattcaa aaatttgcga
     4861 aaatgagtaa aactgcatgg attgaaaagc ttgaaaatga cttgagtgtc atgcgattag
     4921 ctccaaagaa attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg
     4981 cggtagacga taaatgtcca gtaaaaatta gtttttcaga tatagcaaca attatcgaag
     5041 gtatttatta tggtgaagtt gatgaagaga tatcttctac aaaagattta ttagattttt
     5101 tggataaaaa caaagatacc tttatagtac taactgatta tgaggaggtt acaacaaatg
     5161 aaaaattatg atccgaatat acgattgggc acacatacga ttaaggtttc tttccaacgt
     5221 tgggattaca aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt
     5281 ttagcattag acgaggatga tttatacgat caaacattga ctgataatcc aattggattc
     5341 ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct tacggatgat
     5401 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
     5461 gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
     5521 cagataaagg cttagaagga gtcacgacga ataataaaaa ggtagtatta ccttaggcat
     5581 tgagtaagga atttatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg
     5641 atatggatac tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta
     5701 aagatgtatt caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag
     5761 aattcgatta ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa
     5821 ttaaacgaaa gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt
     5881 ttttattttt gcaaaaaaca agaaaggtag gaaaactcat gaattatgaa gaaatcgaaa
     5941 acagaaaaaa agtgtcgaaa gaaatggaag aaaagttgct aaaaatgatg aaacaaaaac
     6001 accttaaaag attatctgtc atgcaatata tcaatgatat gaagataaca ggaaaagaga
     6061 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gaggcgaact tatgtaaaaa
     6121 caggttccaa ttgccaacta ttattagaag tttcttgacg attatgatat gaaaatgatc
     6181 aattatgatg tggcattgtt attgaataaa aagaatagta aaatttaaca tggtttatct
     6241 tggttagatg taggtaaatt aattcatata tagaaggtag atagatgaat aaaaacaata
     6301 aaactttatt taatccttac gtgggcgtaa tcattgtttt tcttcttttt agttattttg
     6361 cttatcaagg ttatcaaaag gacaaaatcg ctgaaaaagt agatggtgta acaaaaacaa
     6421 tccatattca ggaacaggct aaaacctatc aagataattt aaccagttta acggatattt
     6481 ctaagagaca actgattact aatcaaaaac aggcattaga aaaggatctc aaaggtgaaa
     6541 ttgctggaaa acttgttttt tcttctacag gatttattct ccctgttttt caaggagcga
     6601 atagcgacac gcttagttta ggagtagctt ctacttatta tctagatact gaaatgggaa
     6661 atggaaatta tattcttgct ggttataacg tagatacacc aagcgtttta ttatccgata
     6721 tgactgattt atctgaagga gaagaaattg atctaatcga tgctcattac aagtatcagt
     6781 atgaagtctc caaaagagga aaaatttctg attacagcca ggtagttcaa tctaaggaaa
     6841 acaattcctt tttatccttg ccaaaagcag gtaaaaaacc actactcaca ctgttagcct
     6901 ttcaagcaaa taatatgaaa aagtgcgatg tgatccagtg tgaattagtc aatgtagaaa
     6961 aaatcaataa ataagggaag tgagtcacat tcaagaactt gtagaaaaat tgatcaataa
     7021 aatgagtgcc atttttctgt tactgttggt atccattcta tatttaatat ggacggtggg
     7081 gttaaatcct cctacgatat tatttattta tgataatgct gcacagttag tgacgttttt
     7141 cgtaggctgt ttgtttcttt tgaatctgta caaggtaaag agaaacgatc tattcttcat
     7201 tgcagcaggc attctttttt accttttctt ttcttttttt agaagctacc gaggagatac
     7261 tttctatggt gatctgatcg ttccgacaat tattgctatt attttgattg taaaatggac
     7321 gaaatttact gtaattgatc gtagcttgta tcttgtagta ttctatgtct ttttactcgt
     7381 aacggtttat cgagtattta ctgagatacc agtaccagaa ggacaaagta tttggaatcc
     7441 atcgaacaaa ttatcagata tttggatcaa tacaaatacg ataggtagtt ctttaatgac
     7501 attagccctt cttatcacag gatttgctag ttcatttgga aaatggtaca tgcgattgct
     7561 atcattacca gcgttgattc ttgctggggc aacgatttgg gtttgccaat cgaaagcagc
     7621 attatatgcg cttgttatct tcattatctt agaaatttta cctaagaagg tttttcgaaa
     7681 tacacacttg tccttttgga tgtatgcagc agtttcggtt ctagctttac cgatttctta
     7741 ttttgctgca atgtcagaca aagttcactt atttacagga cgagaagaaa tttggcgcaa
     7801 attctatgaa acattaggcg aaaaaacagg acaaatattt gtagggatga aacctttctt
     7861 tttccataga ggaaatcaga ttctaggtaa tcataatacg tatcacgcac tcgtcaatca
     7921 ctatggattg attggttttg ttctatcgat tattcttttg cttagtttct tatggttgct
     7981 cgtaagaaaa cgtgagctga caaatggaca actaacattc ttgtgggctt ttttagcgat
     8041 catgtgtcaa tcgttcatgg aagaaacatt aacgacaatc agttgggtgc caattgtatt
     8101 cttaatgtta gcaatggcta cacaccaata taaaaccacc agataaatga aatagaatag
     8161 taataagaga aaacactttt tcgttgtgaa aaagtgtttt ttgttttgtc tgaaaggagg
     8221 cagatggatg aaaaaatggt tatttggttt tttaggagtc gctttaatcg tcgtttgcag
     8281 tgtgtttggc tatgttagct accaaaaaca tgaaggagag gtgtttaagc aaaatataga
     8341 aaaaaagatg cccgtagatc aaatcaatgc acacgcaaag agttataagg aggatgcaac
     8401 gaacgtcaat aatgatatgt ctttggggca aatgttgtcc atacaaaaag aagcaatcga
     8461 aatgggcgtc aataaacaag tatttgcaca aattcagatt cctgcacttg gtttagcatt
     8521 gccaattttt aaaggggcaa atcaatacac tttgagtctt ggagcggcta cttattttta
     8581 tgaagatgcc gaaatgggta aaggaaacta tgtcctagca ggtcataaca tggaaatgcc
     8641 aggtgtttta ttttctgata ttcaaaagtt gtcattaggg gaggtgatgg acttagttag
     8701 taacgatggc gtctatcgtt acaaggtaac gcgtaaattt atcgtacctg agtactttaa
     8761 attaatcgat ggcgttcctg aagagaactc tttcttgtct ttaccaaaga aaggagagaa
     8821 acccttatta acgttgttta cttgtgttta tacgtcacaa ggaaaagaaa gatacgtggt
     8881 acagggagag ctacaataac aagaatttaa tcaacgcgag tcgttgaaat atgaaatgag
     8941 gagagtaaaa aatggcaata aatcggtatg taaaaaaagc tggaatgatt gttggaatgt
     9001 taggaatcat tgcaggctgc tttcaggggt atcgagctta tgcagaagat gtcacgcaaa
     9061 aaacgccacc agaaagggtc aatatcacgg ttcataaatt aatgtacgat caaagtacgc
     9121 aattgaacgt tgatattgac ggaatcaaaa atgatggtta tacgcacgat caatatccta
     9181 caggtgtaac gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata
     9241 ttacggatca agtattaccg actgaagatt ctgatctaac caacgcgaaa gtggacgaaa
     9301 tcgttaaaga tgtagaagac aagggatcta acagtgagta cgttaagaat gcaaccaata
     9361 aaattacttc agctgtagat gaaaatggtg agatcacttt tgctgatcaa ccagcctatg
     9421 tcaattccaa agggaatgta tatgtggttt atgaaagtaa atccgctgct ggattagtca
     9481 cacaaaaagc aaaaccaatg gttgtgattg cgccaatgac agacaacaca ggatcaggat
     9541 ttttaaaaga tattcatttg tatccaaaga acattgtttc taagttgagt tttgaattaa
     9601 cgaaatttgg tgacgatggc acagctcaaa gcaaacaaac acctttaaaa ggggcaaaat
     9661 ttgagttata caagggcgaa cctggaaaag gaacaaaatt aggagagctt gtatcggatg
     9721 atcaaggaaa gctgactgca actgatttga cgttaggaaa atactacttt gtggaagttc
     9781 cttcagaagt tgtagttgga tcagatcaag aaccaacagc agaccaatat ttgttaggcg
     9841 cagacgctcg caacgatgcg cacaataagt taacctttga aattacgaat gacggcgtga
     9901 caagtgattt aaaagcttcc tacgtgaact ataaagcacc agtgatcgat aaaacagtga
     9961 caaatggaac aggagaagaa cattctttcc aaattggtga cgcagtgaat tatcaaggta
    10021 caattcatat ccctacagat atcgcaggtg gaacagatgg gattacagta aatggtgtaa
    10081 aaagtgaaac ttctccttac agtgtattta aatggggcga cacagcaggt aaaggacttt
    10141 cttatgttgc tgaaaaagca gatattaaag taacaaataa tgatggttca gtcgtattaa
    10201 aagaaaatac tgactataaa atcgaaaatt ctgatagtgg ttttgtgatc gattttatcg
    10261 taaataatgg acaagtaagt gatacagttg cgaatttaca tggtcaagat ctaaaaatga
    10321 cttataacat gtttgtgaat gatagtgctg aaatcgcaaa tccattaaca aatagcgtgg
    10381 atttcgttta taacaataat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg
    10441 acgttgtgac ttatggcgca aaattcttaa aagtcgatag cggcctattt ggaacaggta
    10501 tcaaagcaac accgcttgaa ggcgcagaat ttgcagctaa gaatgcagaa ggtaagtact
    10561 acggtggact tactgataca gataaagatg gtgtcaaaga agcagtttgg gtagacgatg
    10621 tagcaaatgc agcaatccta aaatcagata aagagggtca ttttgaaatc actggtttaa
    10681 ctgaaggaga ttactcactg gaagaaacaa aagctcctga aaactatcaa aaattaacaa
    10741 aagagatctc tttcaaagta gacaaagatt catacaaaga agaaaaccga atcacgatca
    10801 aaaataatca aaaagcttcc gtaccaatga ctggtagcaa cggtttccaa tcttacgtat
    10861 tgatctcttg cttgttgctt ggggcaggcg caatttctgc agttgtttac tttaagaaga
    10921 aagcctagaa ggatcagcta atgattatca gacaccctaa gaaaaagcga atcatgggta
    10981 aatggatcat tgctttctgg ttactttcag ctgttggtgt cttacttttg atgcctgctg
    11041 aagcgagcgt ggcgaaatac caacaaaatc agcagattgc cgcaatcgat cgaacaggaa
    11101 cggcagcaga aacggattct tctttggatg ttgctaagat tgagttaggc gatcctgttg
    11161 gtattcttac aattccaagt atttctttaa aactaccaat ttatgacgga acttccgaca
    11221 aaattttaga gaatggtgta gggattacgg aaggaactgg cgatattaca ggaggaaacg
    11281 gtaaaaatcc tttaatcgca gggcacagtg gactttataa agataatctt ttcgatgatt
    11341 tgccttcagt taaaaaggga gaaaagtttt atatcaaagt tgatggtgag cagcacgctt
    11401 atcaaatcga tcggattgag gaagtgcaaa aagacgaact acaaagaaac tttgtcactt
    11461 acttagaacc aaatcccaat gaagatcgag taacgctaat gacatgtacg cctaagggga
    11521 tcaacacaca ccgtttccta gtgtatggga agcgagtaac ctttacaaaa tcagaattaa
    11581 aagatgaaga aaataagaaa caaaaattat cttggaaatg gcttttagga agtactgttt
    11641 ttctttcagt catgatcatt ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg
    11701 taaccaacat gagaaataga aaatggctat ttgtttgtgc agccgctctt ttttctttca
    11761 ctgcctacac acagttgacg tttgcagaca gacaagaaag cacggatagc acagaacaaa
    11821 caatccaaac gtcagatagc agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa
    11881 cagttgattc ttcagaagaa cctaaaattg ctgaagacga tcaaatagtc aaacagacca
    11941 ttcacgttca aaaaattatt tcttccgaac aattagacaa ccaggggcaa aagcttgaaa
    12001 aacaagaagg agtaaatggc gcgaagtttt ctgtttatga tgtatcggac attttacaaa
    12061 agatggatgt taaggatctc actactgacc aaattgaatc ccaattaaaa gatcgagtga
    12121 aaaaactatc cagtgatcaa ctgaagctaa tttctaatgg agaaacaaag acaattgatc
    12181 aacaaacagg tgtctttgaa ttctcggtag aagtacaggc aaatcagaaa caagcttatt
    12241 atattgtcaa tgaatcaagt cctgaaaaca tttctaatag cgaggatatt ttgctattaa
    12301 cacctgtttc tgataaaaat ggtgaattct taaaggatgt gtggatttat cctaaatcag
    12361 aagcttcgca acctaaagaa gaagtaaaaa aaatcgtatc tactggcgta aagaaaaact
    12421 tctttgagaa ttgttgggat tttgttactc acttgttttc tagagattaa ggaagaaata
    12481 aaatgaaact gatcaaatta ttattattca atttttcttg tttaaccgca gtctttttgt
    12541 actgcggttt attattttct aaactatcta ttttgccaag ctaccaacta tacctaatga
    12601 ggattttaaa cataccagct gcttccatgc cgctagatat tatggaaaag ctcattagga
    12661 atccattatt tactacgatc aatttgatcg ttattgtcgg aatttcacta gtcatttata
    12721 aaaaagaggg gatgaaggga tgaattttaa acactcgtgg acaagacggt taaaatatgt
    12781 ttttcccata atgatgatgt taggtacatt gtttgggtta aaaacagcca atgtctttgc
    12841 tgaaaaagtt attgtcgatc cgtcaaatcc aatccagaac gtaaagaata attttattat
    12901 tccaaaagat gttcctaatg caaaaacaaa tattactgta aacggagcgt caaaagttta
    12961 caaatatcat gttgatgcca acaaaggtgg ttttacagac gactacgtag gattagtgga
    13021 gggaaacaaa aatatacgct atccttcttt ccaaaaagaa tacggtgaaa caaaccttgt
    13081 tttagaaggc gtaacaacca acaccaaagt caatattgac tatggaaaaa tcggtacgta
    13141 taacgggaaa aaagtcaata tcaagctagt tctatctaat attcacttat actcggatac
    13201 gttgccgtgg aatctactag ataacaacta tacaaaaact cattttcgag acgatggcta
    13261 taaaaatacg aatggtgcaa tgagtaaatc taaaaaaaga acagtacttt ggatctcaga
    13321 caatcttttc tcaggaattg tttaccattc aacacaaatg aatgtgcagc tagtagccac
    13381 atatgaggac ggtagccccg ttcaattttc aggagataca tttatcagtt ttaactcttt
    13441 gaaccctgca ggaggaaaaa gcacggatct taaaggagaa tatgcccact atgacaaaat
    13501 gaatacgaca gattggtatg tcagaaaaga tacggttctt tcagaattta aatctttcta
    13561 caacaatctg aacgtggtag gtggccaccc aggaggaagc tctaagctga cacaagcgga
    13621 taatgatttc aataatttgc atgataaatt aggagatccg aagtttggtc aaggtactgt
    13681 gtcctttaaa atatctgaag caaatccgac gtttgtgatt ggttcttcta atgttcaaac
    13741 ctggtttact ttatcttctg cgacaatctt tagtgttgtt cctgatcaac ctgaaaaaac
    13801 aggggttgat aaaaatggaa ataacgtgaa cgataaaatg ttgcaagtag gagacacgat
    13861 tcaatatcga atcaaacaga aggtgaatcg attaggagtg gatttactag cggtttacga
    13921 tcgatttgag ttaatcgata atttaccaaa agaagtaaat tacgtcgatg cacacgttga
    13981 aagtggtaca aataaaaaat tcgatgtatc tggtgaagtg acctatgaca aaacaaaaca
    14041 ccaggtaaaa tatgcagcga aagctgatac attaaaaaat cgaatgaagt ataacggcga
    14101 aacttacgaa ttagtgatca atgtaaaagt caatgaattg gccaatcaaa atagtgtagc
    14161 caaaaatcag ggaacgtcga tcattaataa agtagaaaag gatacaaata tcattaccgt
    14221 ttatttccct aaaattccag tgaaagaagt acaacaaaat ggaaaagatg taaacggacg
    14281 aaatgacggt aagaagggta cgccgacagc tcctttaaac gcaggatctg aagtacaata
    14341 tctagtcaca caaaaatggc acacaaaagg cgtagacgct gcgtcagatc actacaaaca
    14401 atttagtatt caagatccaa ttgaagctcg tttgacatat aaagaaggtt ctgcgcaagt
    14461 aatagataag agtaaaggaa aagatattac ttctgaaggc acactaacct atgatagcaa
    14521 ttcaagaacg ctgaagtggg aagcttccgc tgacttctta aataacaatc ttttagatgg
    14581 acgagaaatt cagctgatat ttacagctaa aactccatta caatcagaaa aaaatattga
    14641 taaccaagcc gtagtagcag tagataatgt ttctaataaa acaaatgttg tgacaattgg
    14701 ggttgatcct aacttgccac aagttattgt tcctaaaaca ggttctacac atttagtaac
    14761 gattttagcg gtcagcttac tattacttgt gttagcaact tttggctacg cagttctgaa
    14821 gttcaattaa acaaaaaaac aaggctgaaa atcagccttg tttttctatt tgaaaatggg
    14881 attggcacta ataaatacgt tgtctgaaga aagtgatccg tctgcagaaa cttcataagc
    14941 atgggataaa ccaacgatac tttgattgtt gtaaagcaag tttgcgttat gagcagcgct
    15001 atctttccac ataaccatag catttactgc tcctgtatct gaaaggatct cgtcaacgta
    15061 aaggtagaaa ctattgatat tttctccttt agcaccgtag cagcctaagc tttctaagag
    15121 atcttggaaa gtaccgccgc caggtcggta atgactgaag cttacttgta attcttttgc
    15181 acgaatatct gcagcttgtt gaacgatatc attgaattgc attggggcaa cgccattttg
    15241 ttgacggtag ctatttaacc aagcaagttc tttttgacgc acatagttgt ttaaatcact
    15301 tagtgagata tttttgtatt tgaaagtgat cgttcgattt tggtcaacat tattgattac
    15361 acgttgtcca tttttatcaa cggtaacaga tgaatcgtca tttctacaca agaagtagtt
    15421 ttgatcggtc atatttttcc aagaagtaga gaaactatct ccctcgtaaa ccgtttgtgt
    15481 ttcagtagat aaaacagcgt ttgtgtcaac gtttatatgg ttcacagtga cgttgtattg
    15541 atgagtatag gcaaaattga ttccttgatt acttgtaacg ttgtttaaag tcacagtgtt
    15601 gctagagctt tctctaggat caagaacaaa attagtgaac ataggtgctt caattgtgat
    15661 gctttgtcca taaacgacgg gataactctt actgtaatta tagagttctt ggatatttgg
    15721 atcagtatcc gagactttat ttggacgagc tttcacttca actagaaaga cttcatcata
    15781 gtaaaaagta aatgtggtat ttgtgttaat cgtttgtgtt tgtgtttcat tcccatttac
    15841 ttgatactgt ggtgcttcaa agttagtcaa gtcggttcta gcttttgcag tgtaacttgt
    15901 accagctttt actgagacat gttcttcttt tagcagcgtt ccagtttgtc gattcatatg
    15961 acggacagtt accgcatatt ctgaaggttt aggtttgtgt tgtaaccctt taaatgcaga
    16021 atcaagacta tttttagcat tatttacttg ggtttgagaa gctttagaat cgtctagtac
    16081 ttttttagca ttggataaag cagtctgaaa attattccaa gatccttctg taaaatctcc
    16141 tttagcagtt gattttactt ggttgtacaa ggcttgtaat tccgttttgt tcacagtagg
    16201 agctggtttt ttctttaatc ccttgtaagc agaatctaat gtgtttttgg cactatccac
    16261 ttgtttttga gtcgcttttg agtcatttaa cactttttta gcattgttta aagcggtttg
    16321 gaatgcgttc caggtattgt cggtatagtc acctttttta gttcccttaa cgctcgtata
    16381 aagagcttct aaagctgatt tattcacttt gggtttagag gtgtcagcac cataccaacc
    16441 aataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
    16501 agtgtagtga tgcgaaccag aggtaacacc tggaaggaaa aggcggtaga tcggtttatt
    16561 atttttttcg gcagaacgcc aggcaggccc ttcataagtc caaccgtaat ttttggtcaa
    16621 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaaggcctt tattgtataa
    16681 acgatagaca aggtggccac cgctagtcgg tgcttcccaa gcggggcctt cataattacc
    16741 ccacttaatt ttcactaggt gatcacgttc ttttgtgcta gaggtgtaaa aatgttcatg
    16801 gttatttgga ttataaaggc gatacatgac gttagttgaa gcagcgtcag cagatccagc
    16861 aagagcaact gtggcagcaa atgttccaat agctataaca gtttttgggt ttaataattt
    16921 catgttttca actccctatt aaatgattaa atattttgtt tacattcata atataccacg
    16981 gtataccgag gtagtcaacg gtataccgtg gaaattttga aataaaaaag ccgccctatg
    17041 aagagtgtaa ggatggctgt ttatatgcta tgaagaaatg gattcggcgt atttggttag
    17101 tttggcagca ttatcaaatc gcattttttc aatcgcactt tttttcttta ttaaatccgc
    17161 aattgttgat tctgcgactg aagaatgttt gctaatttca tatgctgtct tgccagtttc
    17221 aaatagtaac cattcgattt tctctttatc agcgtacatt gcaatgctcc ctcttgattt
    17281 tttctttatt atagctcttt tttgtaataa atcacaatat caagaggaaa agtttctata
    17341 gaaaggtggg aaattatgag tatccgttcc ccgtgtatca aaatgaggtt tgttgctact
    17401 ttcgttattt aatatactaa aataaaacaa cgaaagaagg aatagaatga aaaagttaat
    17461 aatggtattt acttcgttga ttgccatgat tgttatttta ggtggatgtg gtagtgaaaa
    17521 aaataagcaa gatagtcaag ataatggcgt gttaaaacca ggaacagtgt ggaaagaaga
    17581 agtaggcgga cttgttcata atttaaaaat tatagatgat acaacatggg aatattccga
    17641 aagtgtttgg catcctgaac aagtacaaat aacagttgag aagctaaaag actataaagg
    17701 attagaaaga tataaggtaa tcgattcagg tggagtagat ttattcatag gtaaaacagc
    17761 cttaatagtt ccctataaca aagacggttt agaaacgttt aaatttattc caacaaacga
    17821 aaaagaacat cgagataaaa gtgaaatatt aaaagaatac agtactaaat ctggatacaa
    17881 gttacaaaag actagcgagt gattgctagt ctttttattt atcctgaagg aggaatcgca
    17941 gtggcaaaag ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgtcgcggt
    18001 gtatctgaaa cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg
    18061 ggaaaagaca aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc
    18121 gatgaaatta ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga
    18181 attttttaac acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaggtg
    18241 cagcaactag tttaaaaaac aatctgatga tttttgcggc tgtagctgcc gttgcctcca
    18301 ttgttcttgg ggggattttc ttcttctttg gccgaagagg agctgaaaac gggaagatgc
    18361 acgtaggtta tgtattgatg ggggtaggag ttatattagc tgccgcctct attgtggttt
    18421 cgttatttgg aatgtttgga caaaccccac agggactata aaaaataaat agaaggagta
    18481 aaaagtacgt ggctactttt tactcctttt ttatgaaagg agaatactca tgtactttac
    18541 gatttcaggc aataccaaaa aagataaaga gttaataaaa aaagcaacga agaaagtggg
    18601 aaagcgtact gcctttttac cgaaaacaat taaatattct gtcattgaaa cggaagaaca
    18661 gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta aattgactct
    18721 cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg tttttggccc
    18781 actggattat caaatttcag aaggttttta tcctacattg atggatttga tccgccttga
    18841 tgtgtttaag gaaacggatg aaaccatgag tgtggaagag tcttatcgaa acgaaaaact
    18901 gctacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgagtc aagtgagtga
    18961 agtcgaagta aatgatgaag taccatggga acgaccaatc tttccaaaga aggagtcaaa
    19021 agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga aatcgatatt
    19081 tgaggaagag aacgatgtaa tgggtcaatt ggaggtagat ccctttgaag aaacaatgga
    19141 ggatgaagag acggccgaag tagaagttga tctagttgaa gctaatgaag agccaacatg
    19201 gactgaaggg gattctcagg aatatattga accacttatc agtgaagaag tagagaaaaa
    19261 cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat atccgccaga
    19321 gcatgaatgg atgaaagaaa aaaataaatt atttttaaat gagctttatt cgaaatatca
    19381 attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct cacagggtaa
    19441 aggcacactt ttagaacagt tagagtctgt tcaatcaaca aattggcatg agcttgcaca
    19501 agaagattta ttagccactt ttgaagaacg tcaacaagaa acagatcaag aaataaatga
    19561 gtatgcgacg gaacagtcat ctaattggga gagaacaaaa gaggaactaa ataaagaaga
    19621 acaacgagtg attgaagaag aagtacaaaa aattaaagag cgatacgaaa agaaacgcga
    19681 caatgcgtat tccttgtgta tagagaaaat taataacttt actaaaacta atcaagaagc
    19741 tttatcgaaa gaaaaagaaa acttgttaaa agaacgtata aaagaacgaa aagaagagtt
    19801 ctaccgaggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg aacaactgaa
    19861 taaactttat caaacaacga ctgaagttct tatggaaaga cacgggcaga tccagcaaga
    19921 attacaagca caaattccaa aatggaaaaa agagtatgaa attcaacaac aagaattgaa
    19981 agaacaagaa gaaaaacaac gacaagaaga aaaagagcgc gaacaattag aactaaaaaa
    20041 acgtgagcag catcttgcag aacaaaaagt ggcgatagaa aaacaacggt tagagcaaga
    20101 aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata cattgcgaca
    20161 agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa cgccagtgaa
    20221 caaccaagca aatttaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    20281 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    20341 tcaatcttca caggcagatt ctgtagccac aacggcgtca atcaaggaag aagcccagga
    20401 acaagcaaga aaagagtatg aacaacaatt ggaagaatta ttgaatcgta ccaatcagtc
    20461 aacagcagag tcaagtatgg aaaatagcaa agagagtgat cctagtaagg ctaaagaagg
    20521 atcaagcgat tcaactgtac actcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    20581 ataatgcgta ttagagaaac aacgagacga aaagacaaac gattcaattt agaaaagaat
    20641 aggtatcttt ctcatttcta tcgctatact ccttcaggag aagagctacc agaaaaacat
    20701 gacaaacgta tttctcaaaa gattttttat agtggcttag cttatcttcc tttaaactat
    20761 gttgttacaa gaataccaga agcatttcat cgatttgaga ttattccagg aagtttacaa
    20821 ggagtgaata tttttcagtt acaagaaatg ccgcagttat tccattttga ttcaactggt
    20881 ttttggaatc ctttgacccc aacaacaagt ttaggcacac gtttagttgt ttctacgatt
    20941 tgtgttgttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tccgctagca
    21001 gatgggcaaa aaggagataa ccgattaaaa acgttaaagg aaatccaaca aatttatccg
    21061 caaataccag aatcaaaggg aagttttcct ggttatggcg gaataccaat tactcattat
    21121 aaaaaatact ggtatattca gacggataca gtaaatactt gtattgttgg tacttcccgc
    21181 tcagggaaag gccagataga agttttagca accattgaca atttgtcgcg tgcagaaaga
    21241 caaagttcaa tggttgttaa tgatccaaaa acagaattat atgttgctag taaagatgaa
    21301 ctagaagcta gagggtatga tgtgtatgcc tttaatatca ccgatccact gcaatcaatg
    21361 agtgataatc cattggcact cattgtgaaa tactggaagc gaggagacat agacaccgca
    21421 acacagttaa ccaatacttt tacgaatacg atttattatg atgcaaatgc ttcagacaat
    21481 aaatacttta acgaaaatgc gcagaaagct gtcaatgcgc ttatttttgc gttattggaa
    21541 caagcggatc aaacagggga ttactccaaa ttaacaccaa ataatctcgt tgaactactt
    21601 tcagaaattg gcggttttaa ttattatcca gatcctgaga atgaattcaa gcagctcaat
    21661 gccttagatg agttcatgaa tcagttaccc cctggaaata tagcaaaaaa acaatatggg
    21721 gcaacaaaaa ttggatctga taaagcgaaa gggaatatct tgtctacggc aattacagga
    21781 ttgaatccat ttaccctaac gaaaattgcg aagatgactt ctcaatcgac cattgattac
    21841 aagtccattg gttttccgaa gtatttggat ctcaaattac atgaaagcct attaaatcaa
    21901 cgcattgtcg tggaatttta tcataaggga aagaaatttc acgaggaacg agtcaaagtt
    21961 ggctatcgtg gtttttgtga aatcaacttt gattgtcatt tagagcaggg agatcatgtt
    22021 tgcttaaaat acaatcatca aggaaaacaa tatgttgctt ggtatgagtt ttctcaaaaa
    22081 gtattgaaag acgaagaggg aaaaatcgtt tatcgaaaaa aacgaggaga aactcatttg
    22141 ccagaactag aaaaagaagc cgttctaaca ttagatgaga agaaaagtaa cctattagta
    22201 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgtt aacgcctgat
    22261 tatgatccat ctaaccatat cattgttagc atctttctta gtcagttgta taaagaatta
    22321 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gtaggattca ttttatttta
    22381 gatgaatttg gaaatatgcc accgatggac aacatggaag gaatcatgac ggttacggca
    22441 ggtcgtaata tgttatggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    22501 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    22561 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    22621 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    22681 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    22741 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    22801 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    22861 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    22921 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    22981 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atcttttgag
    23041 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcaac ggatcgtctt
    23101 caagaatatg ttgaaaagtt gatcaaaaat gaagaaattt ctgaaatgat taggaaacgg
    23161 ctgttattgg aagctgaaaa ggcggtgaaa gaatgatcat tggaggaatt ttctctggca
    23221 tagccgactg gtggtcaggt ttattcggag aaggggatgc agatcccaaa accgtaacga
    23281 aagtcttaca agattatggt aattatctgc agtatcaaga tgtattcagt tggatttgta
    23341 atactttttt atggggattg atcaaagggt tatataatct caatacttta ttagaaaaaa
    23401 caatttatca aagctttgat ttaaaagact tattgaacgc cgctggtgtt aatgaactat
    23461 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    23521 gcttgaaatt ttttatgagt aaacacccac cgaaagtcaa aaatatcttg gttaatttat
    23581 ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg ttaaatatca
    23641 gtaaaaactt ttatggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    23701 cttttcaaat tattcaaaat aatgtgattg atgtcagtac attactagcg actgacccga
    23761 ataaagtaga atccttacca acagataaaa gaaatgcttt aacagcgaaa aatttcaaaa
    23821 cagcgaatat caatgggatt attacacctg atcaagctaa gaaaatgtct gaatcaagtg
    23881 atattagtac agtacaaaaa gatcggttaa aggcattaca gtatcgacta gagttagacg
    23941 ataatgggaa agaaatccct gttcaaatta gtgatgaagg tttagcaaaa tatgtgtata
    24001 cgtcaggcta tcgtcgatac attagtagtc cagggattat tttggctggg gaatcttctt
    24061 tgtcggtagc ctatttattt attttgttta caatcgttac ttgcattatt gagctagtat
    24121 ttaaaaaatt ctatttagta attgccgcgt caacggactt tgagacagga caacggatga
    24181 aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg attttagaat
    24241 tacgaatcta tacattaatg ctttcaggta tcggtgattt gcatgcggta ggaaagatca
    24301 atggattctt gtatgttgtt gctttaattg tgctaactat tgcgctattc aaaggctctc
    24361 aatcggttac gaagattttc ggtgttgata cgagtctgaa aaacggcagc aattctcttc
    24421 ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct aaaaaccttg
    24481 caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaagaat ggatttggcc
    24541 atcgaaaaga tattacccat gataacgggg aagaacaatc agacgataaa gctacaactg
    24601 tacctaatag taagaagaca gtttccgaaa tgttcgggaa agcgaaaaat ggtgtttcta
    24661 aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca gttgaagatc
    24721 ttacagaaaa agccaaagac accttagaag agaaattaga cggcacgaga gctaaagaat
    24781 ttgctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa aaagcttcag
    24841 cagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca gcagtgaagg
    24901 caaatacaac aagtgaaaaa gaaaatcaag aaggtgcagc gattccttca gccaaagatc
    24961 accaagatga gttacattct aacgaaaatt cgatcggaga aaaagattca gaagaaggac
    25021 aacatggtca gttaccaaaa accgatccag aacaatatcg ttataaatct aacattaatt
    25081 ctgaaaacga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta caaacggatg
    25141 aggattccat cgatctgcca aatgataatg gcatcgaagt tggaactgaa aaatcaacga
    25201 aaattggttt gcctaagaca caaaatttac caatgaaaga tagtcgtttg aatcaattca
    25261 aagagaatga tcaaagcttt gttcatcaat cacctgcagg tactgttcaa atgaaatcaa
    25321 gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttagct gaaagtggtg
    25381 aaacagagaa tgcgtccgtt caaaatattg tgacaaaaga agctgcacct aagccaatca
    25441 ataaagttca taatggatac agaccagcaa acgtagcatt tactgcaccg acaaaaataa
    25501 atgtgacaag agaaactaaa aacaggaaca atgaattgaa agaacaacta ttgaaggata
    25561 tcaaaccata aaaggcctat ttaagggcct ttttctttat aaaacaagtt taaaatggaa
    25621 aggagaaaat tgtggaggat attttaatag ctacgtttat aactgcactg ggagcatttt
    25681 taacgatatt tgtggtgact attaaaggat ttaaagatac ggatagttta caaaaagatc
    25741 atcagtctat tttagatggt caaaaaacac ataccgaaag cattattgta cacctttcag
    25801 aaggattcac ttatttgaaa gatgctatgc ccacattcac agtaaaatta acggaaatat
    25861 ttcattctgt tgagcaaata tcgtcagaat tgaaagagca aagacaacaa aaagatcgtc
    25921 aacttggctc acttaatttt aggcaagaaa cgatacaaga atcattggag aatgtaaatc
    25981 gattaaacga tgaaatgcaa cgagttcatt tggaaaatga agaattacgc gcacaaaatt
    26041 atgatttaat tcaaaagaaa agaaatctgg aacaaaaaat tgttgaatta aaagaagaaa
    26101 atcgtcaatt gaattatcaa ttacgacgag gaaaaaatcg ttctcgtgat ttaggaaatg
    26161 aattagagtt gtaatattta gaaaaacgct gtgagaaatc acagtgtttt ttatttctaa
    26221 aaatacaaaa tataaggggt atggttatat tatggataaa tacattcata tgaaaaatcc
    26281 tccaagattt agtggggaat ggaagttttt cgcctttttc tttttagatc tattaacgct
    26341 tggtggctgc tttttttggg cgaaaatagt caataatctt gtccacttac cactcttttt
    26401 tgcactcttg aattatggat tatggcttgc gatagcgtta gtgattgttt ggcgacccta
    26461 taataaccct ggtcaacgcc aaatttttgc gattatcgaa tctattttct ttacggataa
    26521 gaaccactat catgcgttag atcctaaccg aaattatcat ggtacgtata aggaggatga
    26581 ataaatgcga gtcaaaggac aaaattcagt tgataagaaa tccattttgc agtttggaaa
    26641 accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag aagagggagt
    26701 cttaattact agagaagatc attttcagcg ttattatcgt gtcgtttcca ctgacgtgga
    26761 gggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg tcatgcgtac
    26821 gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa cggaacaaat
    26881 cgtgcaaaag cggcagttat tagataaaaa tcgtatggaa cagtcactag gaagaaatct
    26941 acagcgatta agaaaatatg aaaagaaatt agtagaggag atccaggaat taaaacgggc
    27001 agaacaagaa cgtcctgact taagcttctt tttcgttatt gaaggaaaga caatcaagga
    27061 tttacaaact agaaatcgtc agttaatgcg atctagtggc attttagggt taaaaccact
    27121 ctacaaaaaa gaattgatta agattcttta tcgaatgaac aacatgaatg acgaataaaa
    27181 aagatggagg aaatgattca tgctagatgt gataaaaaca gctttttcaa cgcaaagtaa
    27241 tcaaaaggtg attgatccat taaaacaact tggtaatcgt gaaatagaga ttttaaaatc
    27301 aaaaggttat gatttagatt tcttaaaaca agtgatgcca caaggtgggt tgaattttga
    27361 tgaagaatat gtgacctatg gaaatggtta ttcacgatgt gtgacagttt atcattacac
    27421 aaaaaatcct actctttttt ggttggccaa tctcatgaac aatcctcata cagtggccac
    27481 actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaacc gcgcattaga
    27541 tgaattaaat gatcgatcag ataatgaacg aaaagcgact gaccgaaata aaagtgcgga
    27601 agaatacctg gaactagcgg aatatgcccg aaaattgact tctaatgggg aaatttctaa
    27661 acaagtcaaa gtgagagtac atgtttacgg aaatacgatc caagaagtgg atgaacgggc
    27721 gcaggaactg atcgaaaatc taaaagggat ggattatcgt gcggtgactt atctgcataa
    27781 aaccaaacga gattggcaat cgatgtttac catgttcgat gaacaagctg aatggtttgg
    27841 aaatatcaaa gggcaatcac ttccatcgtt aaacattggc ggaggattcc cgttccacca
    27901 ccaaacgcta aaagatccag gaggcacaca atatggggaa accatgacag gcggcccatt
    27961 tgtctggaat gccacgtata aaaatagtgt acgtttatct accaatacct tgcttctagg
    28021 aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc atttagctgc
    28081 aggagacttt atttggggct ttgaaaaggg aaaagacttt attcctttct taaaggaata
    28141 taacggtatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt tagagatctt
    28201 tgccacacgg acgtatgatg aagcatccag tttatatgac gatggttcag tcaaagattt
    28261 gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtagtct atcaggtaca
    28321 gttagtgagt cctcagctaa aaggaacgat gaaagcagaa ttcaagacct acttgaatcg
    28381 tttttatgaa gagtatggaa cagtaccacg cggttttact tcctcaaatt cgcgttcaaa
    28441 tactgatacg caagtcacag ggaaagatcc agaagcttat ccgacgttca aagaattttt
    28501 agccttttta ggacaactgg aactaccagg ggcttcccaa gagaaaaaga atcggaaaga
    28561 agaaatggag tctatcgtgg aatccttatg tgagacttat ggcatgattt ttgatggtca
    28621 tagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg actctattac
    28681 aggcctagat cgaggggtac aacaatgtca gatgtacatg gccatgacgt tgatttggaa
    28741 tcaggcattg attcaaggac gaagacaacg acgcttaatt aaagaaaaac aaatggaaga
    28801 acaagacttc aaacgcttct tggcgatgat tgatgagtgc cataatggtg cgacctgttc
    28861 tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta ttgtaataac tcttataacg
    28921 agaagtcaaa tgagtgggta acgccacaaa gggcttccac tgatactccg acatgcaaca
    28981 agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa gtcggctgaa ggataccgta
    29041 aggtaaaaga aatagtgaat accacgaaag ttctttagag gtaaagggtg tatccgataa
    29101 gtcgggtgtc caaaaaaact taatatggtg agtatgtaca aacgtactga cgaactagcg
    29161 aatgtactgt ccgaaaggca tagctcctag aaatgggaga gacagtgagc aaaaaatcac
    29221 tcattgtgct aacttgtggt tgagtaaaag tcgttgttat gaaagaccat gctgcgttac
    29281 aggcgtagga gaagttaggg ggcaaaagct agcatctaag ttaaggcatg tggatagata
    29341 taagggaaca ggggaagcca tcaacatgga gagtgcatac tcgatgaagt ggtagtaaga
    29401 aatatgtgaa tgacaaataa cttactttag tgatggtgaa agtagagtca cgactgatga
    29461 aggttctgta atggaactgg aggaacaggc tcgattcgtc tgtaaaagtc atgaaacccg
    29521 ttcaaccatg ggattctcgt atgactaaaa aggtcacgag ccaattggag cgtgataaac
    29581 catgacaaaa gaaacaagtc caaaagatag gaagttgcgg catgcagaat attatggcat
    29641 gacggaaatc ttcgataaga tatactccaa aagtttgaag ggagataatt tcaagaatct
    29701 aatgcagatt atcatttctg ataacaacat tgtcctagca tatcgtaata tcaagagaaa
    29761 tcacggtagt gtgacaagag gagaagatgg agtaacgatt aaagatattg aaaatctcaa
    29821 ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca cattataacc ctcggaaagt
    29881 aaggcgtgta gaaattccta aatataatgg taaattaaga ccgttgggta ttccatcaat
    29941 atgggataga atcgcgcagc aatgcatctt acaagtatta gaaccaatat gtgaagcaaa
    30001 attcaataat cacagctatg gtttccgccc aaatagatca gcagaacacg caatagcaga
    30061 ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg gtagatgtag atatcaaagg
    30121 tttcttcgac gaagtcaatc atactaaact catgagacaa atctggtcta tgggtatacg
    30181 agacaaacag cttctcttaa tcattaggaa aatgttaaga gcacccgtag tacttccaaa
    30241 tgggaagttg atatacccaa caaaaggaac accacaaggc ggaatactta gcccattact
    30301 tgcgaacatc aatctcaacg aatttgactg gtggatagca aatcagtggg aggaaaaaga
    30361 tgccaaagag ctaaaacatc attttaggaa aaatggagct tgggataaag gtaatatcta
    30421 tcacaagctc agaaagagta caactctaaa agaaatatac ctagtgagat acgcagatga
    30481 ttttaaaatt ttctgtagaa attataatga agcgaaaaaa gttttctatg caactaaaat
    30541 gtggttacag gagcgtctaa agttaccaat atctgaagaa aagtcaaaaa tcacaaatct
    30601 ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa ttagtgacca aaggtaacaa
    30661 aaaggttgtt aatacgcata taggtacaaa agcacaaagc atgattaaag tgaaactaaa
    30721 aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa gataccttta tctatattgg
    30781 agtctataac agtatggtta ttgggattca taattacttc aaaattgcga ctcattgtag
    30841 tgtagcaatg cgaaaaattg caagagaact cagaccaagt tttgaaatac gactacgaga
    30901 acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa ggatacaagc cttacctacg
    30961 gtcaggacaa atacgatata taagagggtt tccaatatta ccagtagggt atattcaaca
    31021 cagacatccg atgtgtaaaa aaagaaatat taataaatac accgaagaag gtagggaagc
    31081 cattcataag aagcaacaaa ccgtagaaga atggaagata tcgtggttgt gtagcaatcc
    31141 agtgcttagt aagagagcaa cgattgaatt tgcggacaat cgaatttcaa aattcatcgc
    31201 tcaaaaggga agatgtgcag taacaggtga ggagcttatc ttatctgaaa tgcattgtca
    31261 tcatattatc ccttatcatg aaagcaaaag tgatagctat gaaaacctag tgattgttac
    31321 tgaggaggtt catcgggtaa tacacgctac acaaagtgaa acgattgagg aacttctaaa
    31381 atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat gagttgagat taaaagttgg
    31441 taacgaagaa atatcttaat aaatatttgt aggacgggaa aacagaatgt cttttataga
    31501 cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg tggactgggg gaaaagacgg
    31561 agataatatc aaactcttac ctatcagtat cgattaatcc agatttcatt gaaacggtaa
    31621 attatgtcac aaacttccaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta
    31681 cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga
    31741 agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac
    31801 gtcatataga aggattagtt ggtaaatcaa tgacgaactc agaattagaa tccattcctc
    31861 aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt
    31921 ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa
    31981 tgaaagttta atcaaacagt atgttaagcg tcgtgcaaag cggcgcttat ttttgtgggt
    32041 gtttggtact tctgcaggtt tgattactat attgatcact gtgtttgtga ccctttttct
    32101 aatacttgcg gcaggttcta ttgataattc tgattcaagt agtggtggcg aagcttttac
    32161 aggagaatat tcggaaggct tacccattta taaagaaatc aaaggaagag gccctttttc
    32221 tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaag tataaattat taccaagtgt
    32281 gatcctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg caagaaatga
    32341 tctcaattat tttgggatta cgtggtttga tgggtgtctg tttcctaaag gaactgcgcg
    32401 tggaattggt ggcattgaag gtggctggta catgaagttt cccaactcaa aagctgcctt
    32461 ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcttgtg tcggaaataa
    32521 aagccctggc gcaagcttat tgatcttagg acgaggaggg tatgccgccg caggaattac
    32581 tgaagacagt gcctattata aaaattgtat gtctattatt aatacaaata aattgatgga
    32641 gtacgatgag tttgcgatta aacactggag agaaggcggc ggaactggtg gtacgattac
    32701 aggaagctgg ggcaatccat tcccaggaag tagcttagat aaaaattcgt tcagtggagg
    32761 acaacttttt gggaaaaatc caggaggaga attccgacct aacgggttcc atgatggact
    32821 agactttggt tcagtcgatc atccaggaag tgagattcat gcggttcatg gcggaaaagt
    32881 cgtgtacgtt ggcaatcctg gtatttcagg tttgggtgcg tgtgtgatcg tgattaatta
    32941 cgatggcttg aatatggtgt atcaagaatt tgcaaatagt acaggaaatt ctcgagtgaa
    33001 agtaggcgat caagtgaaag ttggtcaagt aattgggata cgagatacag cacacttgca
    33061 tttaggattt actcgaatgg attggcgtca agcacaagga catgcattca ttgatgatgg
    33121 tacatggatc gatccgttac catttttgaa tagcagcaaa aaatagatca ggagaaaaaa
    33181 tatggaaaat aaaaccttaa aaatcgtctt aacagctgca attagcgtta gtttaatcgg
    33241 caatattgcg ttgttttggc agcttaaaga agaacaagag acaaatacct ataaaattga
    33301 agaagtggct aaaaacgcgt ctgataagct caaaaaaaat tcttctgaag cacaggcgaa
    33361 gattgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc agctgaagca
    33421 acaagatggc acaaaagcaa aagaggaagt aactgaagca caaagaaaga cggcagaaga
    33481 gttcggtcaa ttggccattg ataaatcatt aagcagaaat gaattaacgg aaaaactaaa
    33541 agatgtcgca acccaagaag tgatcgacaa attaagtcct gtagacgatg atcaccaaca
    33601 tgatgattac ggaagttact cttttacatt aggtgacgtg caaacttacg ctcaaacaag
    33661 cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat tatacaatcg gcaatcctca
    33721 attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg aaaaacaagt
    33781 agatggtcaa tggaaagtca cagactttag ttatttcact cgttaggagg aagaaaatga
    33841 gtaagaaaaa aggagcaaga caacgtttta aaaaacgaaa aagttatctt agatggctgg
    33901 ggatctttat gattctcggc tttttgattc ttgctagtcc gatttacaca cataaaaagg
    33961 tagacatacc agatgaagta agcacgaacg catttttaaa cacgaaggaa ctgacgatgg
    34021 tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc gacgatgaag
    34081 atcagagctt attaaaagaa ctagctaatc ttaattatcg tgtagaagta aagacagcta
    34141 aaggggatta tcaagcaata aaagccaaag tgatcaaggt gtctgatgat tattttgtag
    34201 tgaagttagc acatgtacca gaagaatata ttgctatgcg attaactatt attcctgaaa
    34261 aaattgatcc aaaggtggat atgcaagaac cgcaagatct gattttttat attcacgaag
    34321 ataaggtcaa agatcacgtg aaagacgaag attatgaaca acatgcgatc aattacaaag
    34381 tgaaaggata caagaaagaa caaaaagcag cacaaaaaca aattgaatcc ttccaagcaa
    34441 ccattgattt gaatgaagaa ttagtgaaga aactaaaaga tcaattgcct tatcaagtcg
    34501 cagaagatca ggaaaatact gaaaataaga ttaatagtta ccagcaagaa attgaaacat
    34561 cgaaaacgca aatgaaagat caagaagaga gcattcaaaa acttcaagaa aaaattgatc
    34621 gtatgacaaa agaaaatatt tagaagaata ggtggagtaa aagaaatgaa gcgagcgaac
    34681 ttggtcggca aagatacttt cgctgagcag aaagtattga ttaaagccta tctcaaagtt
    34741 gtaaaagcta aagaaatatg tcaagaagtg aattatctaa cacaacaata tctgagttga
    34801 agaatggtag aaagagttta gataccttaa aattcagatc atttgaagct ctatatatag
    34861 ctgctaaaaa atattttaca gctgataata aataaaaaat taaggtgggt tctatgtgaa
    34921 aaaaacgtat caaaagtttt tatggattag ctatatcgtc ctatttatcg ttgggtttct
    34981 cgtcagtgtg tccgtgatcg tcggaacgct ttttcttttg ttctctgacg aagatctggt
    35041 acttaagggg ttaatgagta cgttatcgtg tgtcatgtta ctttatttat gtatacagat
    35101 caacgaaatt atgccccctc attggcaaga aaagaagaaa gaatttaagg aagaaatcga
    35161 aataaaatcc aatgactgga ttgaatacac atggcttcct ctgaaaagaa atagcaaacg
    35221 aaaggaattt tgggcattgc aattatggcc aataggaaaa ctttttattt atatggcttc
    35281 acttatggtt actttaatcg gttatttggt ggtggtctat ggggtggcct attacttgtt
    35341 aagtgagcca aaacagatcg cggcgtcaaa tattttcacg gcagtaatga cattaatgtg
    35401 tgtcatgtgc ttttctattt ttagttactt actcgtccaa tttatcaggc aaaatttaac
    35461 aatgagtaaa cttttcaaaa gtttattgga cttacttatc ttaggcagta tgttaacgac
    35521 tctaacagat cggttcgaac aatctgaaaa aacggtagag gggataaaga acttcttaac
    35581 gacgattgat caatttataa atggatatat gtatttgttt gaagtaggct ttattctcac
    35641 tttgtttggt tgtgtattta taaaactcat taaaagatca atcgaaaaag aaatggttat
    35701 tgatctaatt ccgacgctga gcggctatga gtattacgtt gattttggaa agtatgagga
    35761 gttgatccat acggatatcg ttcacacgag tatccatgaa gtaaccgtta agggcatcag
    35821 atctacttta aaagatggac aggagacgaa aacaatcgac cgaagaaaag aggtctacaa
    35881 tttttataaa gttgaagcaa gtctacgata tggcattggg aaattgaggt tagatgggag
    35941 taatattcaa caaaacaaac catatgtttt aggaaaagtg gtgatttaga acttcctatg
    36001 aaagatgaat aaataaggag aatcttttga aaatgattct tctttacaga attgtgctta
    36061 tagttggcac ggagttaaaa atcttaaaga aggagcagtt attcaaagtg aaagaacatc
    36121 ataatcagtt actatgtatt aaaacgggta cgatgaacat tcaataccga acagaatatt
    36181 tagaattaac gaatcaaact gattttttgt cgattcgaga gcaacataag caagaaaaat
    36241 taatggctac ttatagccaa ttgattgatg agtgggaaca attcaaggaa tctaaaacat
    36301 aaaattccat ttaaacacct tattttttaa agagcataag gaaacgtgta cccactttca
    36361 aaataaagtg ggtagatagg ctgtgcctat cacgtttctg aaaaaaataa atgaccccaa
    36421 aattttcaat ttatgtgtag gtttatgggt agcaaaaata tgaatgtggg cagcaaaaag
    36481 ttcatttgtg ggtagtaaaa ttcaaatgtg tgtagcaatg tgtgtagcta cacacattgc
    36541 tacacacgct ataacatata gtgaaaagga gatatttatg gaagtaaaaa taagagatat
    36601 tgatgaaaaa acgattcaaa gaattgaccg aagatgtatt gaattaagtg aacgcaccgg
    36661 gactaaatgg agccgaaatc gatacctaaa agttttgatt gaaaatgatt ttgaccatga
    36721 acttatggat catagaaaag atcaatttga tcggttgtta gaaaaatttg tggatattca
    36781 aacttataat acgaaaattt tagaagaata tattgcaaca aataatcaat taatcgcctt
    36841 gttaattgaa taataaaaat agtattgtga atttataaaa ggaggaaatc aattttggca
    36901 aaagcaacaa gaaaacatgt atatctttac gatatgcata tcgaagtact tgatgaaata
    36961 atttctactc atgaagatat atctaattat agtgaagcag tcagacattt gtgtatgatg
    37021 tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga
    37081 aaagaaattt ctatattaac cgaaatggtt tcagagtttg cggatcttca tttgaaagat
    37141 acaggaatac taattggtac aaattcaact gtctatcaag aagcaaaaga gcgtgtggaa
    37201 gggaaaataa aagcgaatca aacgaaaaag tattctccta agatcaagaa accaataaat
    37261 gaatctgttc atgccagagt agacccgttt gatccaacaa agtttttaaa aagataaaaa
    37321 gtagaagggg aacatgatga ataaatcgcc tgcagtcatt ctcacttgtc aatttacctt
    37381 gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa aagcgctgga
    37441 agaaaaggaa cgacgcacgc cggacgaaga acaggaactg caacgaatca agaatgcttt
    37501 agaggatttt tatattcctg aaggagaaac tttttcggag agtaagacag ataaagaatt
    37561 cagtgaaacg tcgaatgaag ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt
    37621 agaggaacaa gattttacca aatatctttc ttatatgacc cgtcactatg cgttagaaca
    37681 aaagaaagaa ctgacgcaaa gggaacaaaa agaataccaa cggttgaacc aagcaatgaa
    37741 aaaatatcaa acagagccac ataaaaaagc ttcaggaaat cttcctggag ttttttctat
    37801 ggcttcagac gaagtgaaag gaagtgatct gaaggagatc aaacaaatct tccaaagagg
    37861 gcaacaaaaa ggctcaatca tttatcaaga tgttgtctct cacgacaatt cgtatcttga
    37921 aaaaatgggc ttgtacgatc ccaaaacaga taccttagat gaagaaggat taaaagcagc
    37981 tggaagaaaa atgatggaga cattattcga gaaagaacaa ttgaatgaaa caggttactg
    38041 gatggcaacg attcaccgta acactaagca tatccatatt cattttgcag ttgttgaaaa
    38101 agaaaatacg cgtaaacctt acacggaggt tgaaaatggt gtgagttata tcgtaccgaa
    38161 aggtaagaga agacaagcaa cattagacgc aatgaagacc aattatgtac atgagttaga
    38221 acgtttttct tatgaaaagg atcgaatgat tggtatggag aaacgaaatt tattgacgcg
    38281 gaaaagtgat ttgcgtaaca ccttaacaaa agtggtaaaa gagccagcgc gttacgatcg
    38341 acacgcaatg aacttattaa atgaagtata taaaacccta cctgacaaac gttcggaatg
    38401 gcattatggg gatgaaaaac gaaccaaact ttcaccatac acaagagaga aactcgatga
    38461 gttaaccaaa tatgtgttaa caaaagaacc tgaatatcaa gaatatatac agatttcaaa
    38521 atcattgaaa gaagaagcaa aaagtcttta tggggaaagc aagagagaat caaaggatgc
    38581 agaaaaaaat gctctctttg atttgaagaa gcgcacagga aatgccattc taacagaact
    38641 gaagaaacag gatagtcaaa taaaaccttt aatagaaagt atggatcaga tgcaatctat
    38701 ttacaatgaa cgatcctttc gaacgcttaa acagttacca acgaatgaat acaaatatag
    38761 ttacgaagaa ttcatgaagc gcaggaaaca attccaacaa agagttgtca cgcaacggag
    38821 tttgcgtcaa ttaaatcgaa cgattaatgc ccatgaagaa aaataccacg caagtaaagc
    38881 ctacgaagag caacaacgaa gaattcaaat ggaacaagaa agacagtaaa gacagtagga
    38941 ggaatagcaa tgaaacaata tttagatcaa tggaaagtta tagaaggatc attaagagaa
    39001 gaacgaatcg aacaacttcc cgattgttta gaaaaagagc atttgtttca gataagagaa
    39061 atgttgagaa acgaacagtt tgatccaaat caatttttag tcgttgagta ctcagctact
    39121 ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt ttatcattca agaatatgag
    39181 ggcaagttag cgccttatta tactacttgg gaaatgaatg aagaggggat caataacttc
    39241 ccgtgtaaaa gcattgaaga gtctatttca ctcacagaat gttagttgtt aagtgaataa
    39301 aagaagtaaa agagtcctga acgtaattgt ccaggactct ttttttcaat gaattgtttt
    39361 ccgccaattc aatgagtggt gcgatttgcg actacattat atcaaaagaa agagaaggga
    39421 ggaagaagaa atggcttatt caacgattgg agttgttgtt gaaaaaagca gagacaatct
    39481 cgtttttgtg acagaaattc aaacgggcag agcttttgtc gtcacagaca aagcggcaaa
    39541 agcgtatcaa agtggcgata ttttaacgtt aaatctgact acgaagacgt tcgtcgatgc
    39601 ggcagaagat tatcc
//
LOCUS       DASHWH010000021        39074 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.021, whole genome shotgun sequence.
ACCESSION   DASHWH010000021 DASHWH010000000
VERSION     DASHWH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.021"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..305
                     /locus_tag="U2R41_001123"
     CDS             6..305
                     /locus_tag="U2R41_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8888547.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U2R41_001124"
     CDS             378..1739
                     /locus_tag="U2R41_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEM8888548.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U2R41_001125"
     CDS             1761..2021
                     /locus_tag="U2R41_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888549.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U2R41_001126"
     CDS             2035..2787
                     /locus_tag="U2R41_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEM8888550.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U2R41_001127"
     CDS             complement(2881..3486)
                     /locus_tag="U2R41_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8888551.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U2R41_001128"
     CDS             complement(3539..4513)
                     /locus_tag="U2R41_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEM8888552.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U2R41_001129"
     CDS             complement(4891..5610)
                     /locus_tag="U2R41_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8888553.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U2R41_001130"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U2R41_001130"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM8888554.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U2R41_001131"
     CDS             7161..8558
                     /locus_tag="U2R41_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8888555.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U2R41_001132"
     CDS             8639..9079
                     /locus_tag="U2R41_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8888556.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U2R41_001133"
     CDS             9227..9553
                     /locus_tag="U2R41_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8888557.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U2R41_001134"
     CDS             9554..10303
                     /locus_tag="U2R41_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888558.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U2R41_001135"
     CDS             complement(10383..10838)
                     /locus_tag="U2R41_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM8888559.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U2R41_001136"
     CDS             complement(11265..11540)
                     /locus_tag="U2R41_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888560.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U2R41_001137"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U2R41_001137"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEM8888561.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="U2R41_001138"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="U2R41_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM8888562.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="U2R41_001139"
     CDS             14770..17436
                     /locus_tag="U2R41_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8888563.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="U2R41_001140"
     CDS             17800..18552
                     /locus_tag="U2R41_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8888564.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="U2R41_001141"
     CDS             complement(18574..19728)
                     /locus_tag="U2R41_001141"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEM8888565.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="U2R41_001142"
     CDS             19913..22354
                     /locus_tag="U2R41_001142"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEM8888566.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="U2R41_001143"
     CDS             complement(22684..23388)
                     /locus_tag="U2R41_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888567.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="U2R41_001144"
     CDS             complement(23575..23820)
                     /locus_tag="U2R41_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888568.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="U2R41_001145"
     CDS             complement(23837..24688)
                     /locus_tag="U2R41_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8888569.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="U2R41_001146"
     CDS             24854..25858
                     /locus_tag="U2R41_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEM8888570.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="U2R41_001147"
     CDS             complement(26099..26455)
                     /locus_tag="U2R41_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8888571.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="U2R41_001148"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="U2R41_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM8888572.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="U2R41_001149"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="U2R41_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM8888573.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="U2R41_001150"
     CDS             complement(30815..31576)
                     /locus_tag="U2R41_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8888574.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="U2R41_001151"
     CDS             31854..32375
                     /locus_tag="U2R41_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEM8888575.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="U2R41_001152"
     CDS             32434..33294
                     /locus_tag="U2R41_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEM8888576.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="U2R41_001153"
     CDS             33479..35794
                     /locus_tag="U2R41_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8888577.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="U2R41_001154"
     CDS             35976..37817
                     /locus_tag="U2R41_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM8888578.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="U2R41_001155"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="U2R41_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEM8888579.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12571 a         6876 c         8095 g        11532 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttt
//
LOCUS       DASHWH010000022        37144 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.022, whole genome shotgun sequence.
ACCESSION   DASHWH010000022 DASHWH010000000
VERSION     DASHWH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.022"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..1229)
                     /locus_tag="U2R41_001156"
     CDS             complement(99..1229)
                     /locus_tag="U2R41_001156"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEM8888580.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U2R41_001157"
     CDS             complement(1878..3080)
                     /locus_tag="U2R41_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8888581.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U2R41_001158"
     CDS             complement(3140..3343)
                     /locus_tag="U2R41_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEM8888582.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U2R41_001159"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U2R41_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U2R41_001160"
     CDS             4880..5692
                     /locus_tag="U2R41_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888583.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="U2R41_001161"
     CDS             complement(6716..6868)
                     /locus_tag="U2R41_001161"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM8888584.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="U2R41_001162"
     CDS             complement(7059..7937)
                     /locus_tag="U2R41_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8888585.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="U2R41_001163"
     CDS             complement(7976..9094)
                     /locus_tag="U2R41_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEM8888586.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="U2R41_001164"
     CDS             complement(9091..11190)
                     /locus_tag="U2R41_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEM8888587.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="U2R41_001165"
     CDS             complement(11241..12062)
                     /locus_tag="U2R41_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8888588.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="U2R41_001166"
     CDS             complement(12059..12868)
                     /locus_tag="U2R41_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8888589.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="U2R41_001167"
     CDS             complement(12889..13383)
                     /locus_tag="U2R41_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8888590.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="U2R41_001168"
     CDS             complement(13395..13883)
                     /locus_tag="U2R41_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8888591.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="U2R41_001169"
     CDS             complement(13960..16656)
                     /locus_tag="U2R41_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8888592.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="U2R41_001170"
     CDS             complement(17084..18157)
                     /locus_tag="U2R41_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888593.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="U2R41_001171"
     CDS             complement(18154..18981)
                     /locus_tag="U2R41_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888594.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="U2R41_001172"
     CDS             complement(18982..19788)
                     /locus_tag="U2R41_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888595.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="U2R41_001173"
     CDS             complement(19800..20885)
                     /locus_tag="U2R41_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888596.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="U2R41_001174"
     CDS             complement(20902..21444)
                     /locus_tag="U2R41_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888597.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="U2R41_001175"
     CDS             21702..22691
                     /locus_tag="U2R41_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEM8888598.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="U2R41_001176"
     CDS             22688..23425
                     /locus_tag="U2R41_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEM8888599.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="U2R41_001177"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="U2R41_001177"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEM8888600.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="U2R41_001178"
     CDS             23989..24621
                     /locus_tag="U2R41_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8888601.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="U2R41_001179"
     CDS             24706..25353
                     /locus_tag="U2R41_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8888602.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="U2R41_001180"
     CDS             25358..26341
                     /locus_tag="U2R41_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEM8888603.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="U2R41_001181"
     CDS             complement(26744..28258)
                     /locus_tag="U2R41_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEM8888604.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="U2R41_001182"
     CDS             28396..29202
                     /locus_tag="U2R41_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888605.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="U2R41_001183"
     CDS             complement(29377..29946)
                     /locus_tag="U2R41_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8888606.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="U2R41_001184"
     CDS             complement(30029..32089)
                     /locus_tag="U2R41_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEM8888607.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32356..33834)
                     /locus_tag="U2R41_001185"
     CDS             complement(32356..33834)
                     /locus_tag="U2R41_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888608.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34132..35067)
                     /gene="murB"
                     /locus_tag="U2R41_001186"
     CDS             complement(34132..35067)
                     /gene="murB"
                     /locus_tag="U2R41_001186"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEM8888609.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35140..36060)
                     /locus_tag="U2R41_001187"
     CDS             complement(35140..36060)
                     /locus_tag="U2R41_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8888610.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36173..36925
                     /gene="xth"
                     /locus_tag="U2R41_001188"
     CDS             36173..36925
                     /gene="xth"
                     /locus_tag="U2R41_001188"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEM8888611.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT        11107 a         7694 c         6272 g        12071 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atatcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gcgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt gagacaaagc
    32341 tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt ctctgatgat
    32401 ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa ttatctgttc
    32461 tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag aagtgattcg
    32521 actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa tgtaatcggt
    32581 ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa aatatgatac
    32641 cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca tgaaacgttc
    32701 atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg ctgtactgaa
    32761 tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc tagtcatctc
    32821 aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa gttcttttcg
    32881 tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat caatcaattc
    32941 cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg caagtccaaa
    33001 tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat aagaaatctc
    33061 atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat ccttgatgat
    33121 cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc cgattggttg
    33181 attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga ttcgttttgt
    33241 tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct ttgcttcatt
    33301 catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat ataccccaaa
    33361 taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta actttatttg
    33421 atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg atatgcttaa
    33481 aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct ccgttaacag
    33541 tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt
    33601 cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct ttgttttgaa
    33661 ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt tagacaaacc
    33721 taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt ctaaatatct
    33781 agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact tcatatccta
    33841 cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc atcttttcac
    33901 ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc attttttatc
    33961 ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc tatatttttt
    34021 gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc acaaaaaaga
    34081 gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt attaattggc
    34141 taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca cttctgtttc
    34201 taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca attcaatata
    34261 atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt cagagacttg
    34321 tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg
    34381 tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct gttttgattg
    34441 acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc ctgtttgcaa
    34501 actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt gacgataaga
    34561 aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca aaagatccac
    34621 ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat aaaccgctcc
    34681 accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc
    34741 tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg ttcctttgac
    34801 atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc catcttgaac
    34861 gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc ggcaataatc
    34921 gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag ctggtccacc
    34981 tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa
    35041 gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc aaacgtcctc
    35101 attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat gacggtagtg
    35161 atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt gtttaagcaa
    35221 ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca ttggctcaaa
    35281 cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt ttccatcagc
    35341 atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt tacttgttac
    35401 ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag ccgtttgatt
    35461 ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc ttatctggta
    35521 ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa gatgtaaaaa
    35581 caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg
    35641 gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa agcgccggtt
    35701 gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt cacgaacttc
    35761 atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt ctagacattt
    35821 tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt cgattttttc
    35881 ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt tttgaattct
    35941 ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc
    36001 ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac caatcttcat
    36061 ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta ctaaaatgag
    36121 gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt tattgaaatt
    36181 tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt
    36241 tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag caggacaaat
    36301 tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa aaggctattc
    36361 cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa tgggtatcga
    36421 tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct ttcttgtaac
    36481 ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat tagaatggga
    36541 agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg
    36601 ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa
    36661 tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata acgggtttat
    36721 cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt ggaactaccg
    36781 attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga cgagcaaaga
    36841 tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca gtgatcattg
    36901 tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt
    36961 aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat aataccgaga
    37021 aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg catcaagtaa
    37081 taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc
    37141 agat
//
LOCUS       DASHWH010000023        36853 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.023, whole genome shotgun sequence.
ACCESSION   DASHWH010000023 DASHWH010000000
VERSION     DASHWH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.023"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(324..893)
                     /locus_tag="U2R41_001189"
     CDS             complement(324..893)
                     /locus_tag="U2R41_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8888612.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U2R41_001190"
     CDS             complement(967..2256)
                     /locus_tag="U2R41_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888613.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="U2R41_001191"
     CDS             2610..3458
                     /locus_tag="U2R41_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEM8888614.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="U2R41_001192"
     CDS             3472..4545
                     /locus_tag="U2R41_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEM8888615.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="U2R41_001193"
     CDS             4535..6706
                     /locus_tag="U2R41_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEM8888616.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="U2R41_001194"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="U2R41_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEM8888617.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="U2R41_001195"
     CDS             complement(9009..11195)
                     /locus_tag="U2R41_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEM8888618.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="U2R41_001196"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="U2R41_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEM8888619.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="U2R41_001197"
     CDS             complement(11417..11857)
                     /locus_tag="U2R41_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEM8888620.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="U2R41_001198"
     CDS             complement(11931..12404)
                     /locus_tag="U2R41_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEM8888621.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="U2R41_001199"
     CDS             complement(12714..14180)
                     /locus_tag="U2R41_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8888622.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="U2R41_001200"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="U2R41_001200"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM8888623.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17099..17458)
                     /locus_tag="U2R41_001201"
     CDS             complement(17099..17458)
                     /locus_tag="U2R41_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEM8888624.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="U2R41_001202"
     CDS             complement(17488..17829)
                     /locus_tag="U2R41_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888625.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="U2R41_001203"
     CDS             complement(17826..18500)
                     /locus_tag="U2R41_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8888626.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="U2R41_001204"
     CDS             complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="U2R41_001204"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEM8888627.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20633..21823)
                     /locus_tag="U2R41_001205"
     CDS             complement(20633..21823)
                     /locus_tag="U2R41_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8888628.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21844..22371)
                     /locus_tag="U2R41_001206"
     CDS             complement(21844..22371)
                     /locus_tag="U2R41_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEM8888629.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22418..25207)
                     /locus_tag="U2R41_001207"
     CDS             complement(22418..25207)
                     /locus_tag="U2R41_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM8888630.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25354..25554)
                     /locus_tag="U2R41_001208"
     CDS             complement(25354..25554)
                     /locus_tag="U2R41_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8888631.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26006..26326)
                     /locus_tag="U2R41_001209"
     CDS             complement(26006..26326)
                     /locus_tag="U2R41_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888632.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26627..27745)
                     /locus_tag="U2R41_001210"
     CDS             complement(26627..27745)
                     /locus_tag="U2R41_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888633.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            28216..29523
                     /locus_tag="U2R41_001211"
     CDS             28216..29523
                     /locus_tag="U2R41_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEM8888634.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            29642..30841
                     /locus_tag="U2R41_001212"
     CDS             29642..30841
                     /locus_tag="U2R41_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM8888635.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(30868..32136)
                     /locus_tag="U2R41_001213"
     CDS             complement(30868..32136)
                     /locus_tag="U2R41_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEM8888636.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(32299..32946)
                     /locus_tag="U2R41_001214"
     CDS             complement(32299..32946)
                     /locus_tag="U2R41_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEM8888637.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(33354..33857)
                     /locus_tag="U2R41_001215"
     CDS             complement(33354..33857)
                     /locus_tag="U2R41_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8888638.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(33980..34744)
                     /locus_tag="U2R41_001216"
     CDS             complement(33980..34744)
                     /locus_tag="U2R41_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEM8888639.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            34851..35126
                     /locus_tag="U2R41_001217"
     CDS             34851..35126
                     /locus_tag="U2R41_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEM8888640.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            35842..36792
                     /gene="yidC"
                     /locus_tag="U2R41_001218"
     CDS             35842..36792
                     /gene="yidC"
                     /locus_tag="U2R41_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM8888641.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT        10593 a         7759 c         6535 g        11966 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaagaact gcgagagttc gcagttcttt tttttaaata aacgttgctc
    19201 tccggccaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    19261 gtgactcgga gctgaacggc aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac
    19321 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    19381 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    19441 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    19501 ccgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    19561 cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    19621 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    19681 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    19741 ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    19801 tgtctcgtcc tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga
    19861 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    19921 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    19981 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    20041 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    20101 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    20161 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    20221 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    20281 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    20341 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    20401 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    20461 ggaaaaaggc tgttccgtct cttaattgta aaaatgtgat ttttccgctt gatcgcttat
    20521 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    20581 gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt
    20641 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    20701 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    20761 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    20821 tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag
    20881 aaacacctgg cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt
    20941 tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac
    21001 tgactgctgt cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct
    21061 cagaattccc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    21121 cattgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    21181 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    21241 ttactgccca ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat
    21301 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    21361 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    21421 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    21481 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    21541 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    21601 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    21661 cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    21721 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    21781 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    21841 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    21901 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    21961 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    22021 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    22081 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    22141 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    22201 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    22261 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    22321 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    22381 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    22441 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    22501 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    22561 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    22621 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    22681 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    22741 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    22801 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    22861 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    22921 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    22981 cgatcagacc tgtaagtgct gtctctaaat aatcgatgta tgcttgattg ctgacttgat
    23041 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    23101 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    23161 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    23221 cagtcgacgg caacatagcc gcttggaaat catggagatg aaaaatcatc tgctgcgctg
    23281 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    23341 tcagcaagta ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc
    23401 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    23461 gctgctgaat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    23521 gcagctgatc cagcatctct tttgtcatca tgacttcttc tggatactgt ccgttgggtt
    23581 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    23641 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    23701 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    23761 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    23821 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    23881 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    23941 aacgaacaaa gtcttgttcg taaaacgact gatttcttgc taaaaaccca gtacctctgt
    24001 gcgcgatatc tgcaaagaat gcatggttca agtttgttaa atgtaattca tccaaatcac
    24061 cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    24121 gttctggtgg ttccacaagt gttcctttaa atcgttgcag gtcgatatag tggcgataac
    24181 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatttctt
    24241 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    24301 gtgtagccaa ataactcaat ggcaacagat aaccgattgt tttacccatt cctgtagctg
    24361 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagatc gcattcatca
    24421 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa catcttttct tttgcttgtt
    24481 tttttcttgg atatgtccgc tcgccgtaat ggacggatgt gaatagttcc acttcttttt
    24541 tctgcaaagc tagcccatcg acgatttcta atgaggaatc aagtggctct ggtttttctt
    24601 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    24661 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    24721 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    24781 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    24841 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    24901 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    24961 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    25021 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    25081 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    25141 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    25201 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    25261 tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    25321 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    25381 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    25441 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    25501 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    25561 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    25621 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    25681 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    25741 gattacataa agtgaaaaaa cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    25801 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    25861 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    25921 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    25981 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    26041 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    26101 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    26161 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
    26221 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    26281 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
    26341 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    26401 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    26461 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    26521 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    26581 cctcttcctt aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa
    26641 tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac actttcccgc
    26701 cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt
    26761 caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt
    26821 ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga
    26881 gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt
    26941 cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat
    27001 aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaat gcaaataaat
    27061 aaatcggcca ttgttcagga ttttcgaaag aatcctttaa gtacgtatca acagcttggt
    27121 aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta
    27181 ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt
    27241 cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta
    27301 cttgtttgtt gctgacctca tacaatttca tggagtcgcg gttcaatccc aataaataat
    27361 aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat
    27421 agtattgatc gtctaccggg atactcaaac gatggatata ggtctggtca gcagttataa
    27481 tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa
    27541 ttttttcttg gaacggagac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg
    27601 cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat
    27661 gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca
    27721 ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt
    27781 tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata
    27841 aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca
    27901 attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa
    27961 taaaagaggt ggatcttatg catttaatta tgattttcag tttattaagt attatcagtt
    28021 tatttgcttt cagcgcttcc cgcttattag atgcggaaac acggcctatt cctgtaaaag
    28081 tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct tatcctaaag ttttttcaga
    28141 aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt
    28201 acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc
    28261 attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tacgttcaaa
    28321 gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa
    28381 gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc
    28441 cttgatcaag taaaagaagg aaaactgtca tgggatgata aagtttcgat ttcagattat
    28501 gcagaaaatt tgagtatcac cccagattta tcgaatgttc ctttgcataa agaaaacacc
    28561 tacactgtca aagaattatt cgactctgca atcatccagt cagccaatgc ttcaatggtg
    28621 gccttggcag aaaaaatctc tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa
    28681 ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg catcaggatt gaacaactct
    28741 tatcttggcg aaaaccgacc agaaggcact ggagaaaacg acgaaaatca aatgtctgct
    28801 caagatgtgg ccatcgttgc ccgccatttg atcttggatt tcccagaaat actggatgta
    28861 tcaagtacta cgacccaaat gtttggcgaa aatacgcaat caccagtgga aatggtcaac
    28921 tggaactgga tgctcccagg attcgttaat tacaaggaag gcgtggacgg attaaaaact
    28981 ggtacgactg agcttgcagg tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg
    29041 atcatcactg tcgtattgaa tgcggacggc catgcggaaa acccgagtgc ccgcttcaat
    29101 gaaacagacc gtttgatgga ctactgctat gacaattggt cagaaaaaga gctcggaaaa
    29161 gctaacgcaa gtattcctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca
    29221 gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac gaaacgatat ggatactggc
    29281 aaattaacga tcacgccaac acttgacaag aagcaagtca ctgacaatga gatccaagca
    29341 cctatggata aaggtacaaa aatcggtacc gctacgatca ccttggcaga cgataaatta
    29401 ggctatttag aagacaacaa tgcaccatca acagagatca ttacggataa aacagtggaa
    29461 aaagcgaata tattcgtgat tggctggcga cgcgtcgcag aatttttcga caacttgttt
    29521 taattgaaca ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt
    29581 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    29641 tatgaatcta accagagatc tgatcttttt agttctctat cttattgggg tattctctat
    29701 cgtttattgg cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac
    29761 atttacggta atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    29821 tttgattcca ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag
    29881 gctgctgaat ggtcttttat tcaatgtttt cctcatcagt ttcggcatct acttgtttgt
    29941 cttgctgtat gaaacgcaaa atattttcct tggcggactg atcgccttga tcacgattcc
    30001 attgctactt gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg
    30061 ggtcacagtt ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc
    30121 catttttctt acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg
    30181 gataaatgct ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta
    30241 caactttttg actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt
    30301 catcgttgtg ttaggcgctg ggttgatcaa tggagaaaca gtttctcctc ttctagccaa
    30361 gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc
    30421 catcttattg atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat
    30481 gaaacaatat gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc
    30541 gacaaccact ttggagaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg
    30601 gccataccgc gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc
    30661 gtggcaagcc aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc
    30721 taatgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    30781 catttgcgga ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta
    30841 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    30901 tattggcaaa aagagtatcc cttttttgac cgctacttgc tgggcagaat aaataccgct
    30961 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    31021 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    31081 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatgaagca aaggcgctag
    31141 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    31201 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    31261 tccattaaat gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa
    31321 aatcaatgct gctgccacaa ctacagctcc acctatcgta tttcgccaaa caggatgagg
    31381 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    31441 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    31501 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    31561 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    31621 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    31681 actgattcca ctgataatca agatttcccg ttctgcttta tccaatcgga acaaacgtgc
    31741 tgtattatct gctaacgatc cccccatttg gactgctgtt ccttcacggc caacagaccc
    31801 cccaaataaa tgggtagtta tggttccaaa cagtgtaagt gtaataagcc gcaacgggat
    31861 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    31921 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    31981 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    32041 caagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    32101 taaaatgcgc attggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
    32161 acgcctacta aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg
    32221 ttagcattta aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt
    32281 ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg
    32341 agccaatttc gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt
    32401 tttcgtatac gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga
    32461 tgttttctga cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat
    32521 cccttttaat ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt
    32581 ccagccgatg tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt
    32641 ttttactttt cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac
    32701 gatttgcttc agcttctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa
    32761 aactgtcgtt aaaaatgtat cgatttctgg cagttcgtga tttaatatgt ctcgaatctt
    32821 ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc
    32881 atttaataac tgttctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg
    32941 ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat
    33001 cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc
    33061 atcttatcat cacccccttt aatgcttatt cttctatgta catgattcat tcgataaata
    33121 aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
    33181 agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcggtaa
    33241 taagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    33301 aagctgacca cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc
    33361 gaggattctt tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga
    33421 gccgcttctt tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac
    33481 ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc
    33541 gacaaatcgg tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt
    33601 gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca
    33661 gcacgcgctg ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa
    33721 gaaacactga tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat
    33781 tttttcactg cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca
    33841 cgccattttt ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa
    33901 attgatctaa gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta
    33961 aagcttaata gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc
    34021 cgacattcaa tgattccgca tttcctttca ttgggatata tacgttctga tcagccgaag
    34081 acaagatttc ctgacttact ccctgacctt cgtttcccat gattatagcg aaatcctctg
    34141 ttttttctac tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa
    34201 accctttgcc tttcattaca gggatcactt ctgccaaatc tttttgtaac acaggcaaat
    34261 gataattgct tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt
    34321 tacctaataa aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat
    34381 ttccaggatc ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt
    34441 cagatgttaa cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg
    34501 tcatgacctc atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc
    34561 cccattcttt catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg
    34621 cagcttcttc gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt
    34681 tctttttatg tagttttttt aattctttga ttcttggatt ttttactgat tggatctctt
    34741 tcacattcct cacctcaact gattatatca tggaataggt gcttgagctt gtttcatcct
    34801 aaaaaaatcg gtatcattga tttataaaac aggaaagaaa ggtgacgaac atgcgcaaag
    34861 ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt tcgctatatg accaagatgg
    34921 ttgctgatca gttaggcgtg accggaacgg taaaaaacga agacgacggc tcagtcacaa
    34981 tcgaagcaat cggaaatgac gacatcatcc aaaaatttat cgaagaagtc aagaagtctc
    35041 cgagcccaag tggacgagtg cagtatgtgg atgtccaaga agatccgatg attgaggaac
    35101 gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag cgctttgcct cgagtattaa
    35161 ggagaaacat tttccaaata gcttctcata ttttgaaatg taatgactta ataccgagag
    35221 gcagctgata aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag
    35281 gactggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa tgccgtagat
    35341 gcaagctttt gaacaccgtc gataaggttg cgaaaaagct gatctgcatc aagcattaag
    35401 gactagttgt gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct
    35461 ttccacattt ttgcataaat ttgacttaat accaagaaac tgacttttga acatcgtcga
    35521 taaggttgtt tttgaggcac tttgcctcaa gtattaaaga caggtagtaa aaaagctgat
    35581 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    35641 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttctaagt atttctttct
    35701 tgattttttg tcttttttca aaatttttcg ctacgaaaaa gaacgaatat ttatatgaaa
    35761 tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt tgtgtaaaaa
    35821 ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg atccggactt
    35881 gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg aaaccctgac
    35941 acttcaggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat tacatatcta
    36001 gttgagaatt tcaattggtc ttacggctgg gcagttattg caatgactgt gatcgttcgt
    36061 atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca atctgaaaaa
    36121 atgcaagcgt taaaaccaca agttgaagca gcacaacaaa agctaaaagc tgcaacaaca
    36181 cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tatatcgcga aaatggattg
    36241 agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat cttctctgcc
    36301 ttgtatttta cggctcgcta cacagaaggt atccgtgaat catcttttta cggcatcgat
    36361 ctaggctcac caagtatggt gcttgtggcc atcgcagggg tcgcttatct gcttcaagga
    36421 tatatttcaa caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac gatgctgatc
    36481 gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc cagccggtgt tacactttac
    36541 tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt tataatgaaa
    36601 ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa acaagtcgtt
    36661 actccaagaa aagacgtgac agaatcagca aagccagctg acaacaaaca agcaacaaaa
    36721 caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg aaagcagcag
    36781 aagcggaaat aaaaatatat gacaaaaaac tgtagacaaa taaaaaaatt tctttgttct
    36841 acagtttttt tgt
//
LOCUS       DASHWH010000024        36568 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.024, whole genome shotgun sequence.
ACCESSION   DASHWH010000024 DASHWH010000000
VERSION     DASHWH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.024"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U2R41_001219"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U2R41_001219"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEM8888642.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U2R41_001220"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U2R41_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEM8888643.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U2R41_001221"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U2R41_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEM8888644.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U2R41_001222"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U2R41_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEM8888645.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U2R41_001223"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U2R41_001223"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEM8888646.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U2R41_001224"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U2R41_001224"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEM8888647.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="U2R41_001225"
     CDS             complement(8053..8523)
                     /locus_tag="U2R41_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEM8888648.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="U2R41_001226"
     CDS             complement(8569..8994)
                     /locus_tag="U2R41_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEM8888649.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="U2R41_001227"
     CDS             complement(9061..9330)
                     /locus_tag="U2R41_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEM8888650.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="U2R41_001228"
     CDS             complement(9341..10936)
                     /locus_tag="U2R41_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8888651.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="U2R41_001229"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="U2R41_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEM8888652.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="U2R41_001230"
     CDS             complement(14489..15049)
                     /locus_tag="U2R41_001230"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEM8888653.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="U2R41_001231"
     CDS             15402..16376
                     /locus_tag="U2R41_001231"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM8888654.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="U2R41_001232"
     CDS             16546..17946
                     /locus_tag="U2R41_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEM8888655.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="U2R41_001233"
     CDS             complement(18024..18746)
                     /locus_tag="U2R41_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888656.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="U2R41_001234"
     CDS             complement(18825..19331)
                     /locus_tag="U2R41_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8888657.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="U2R41_001235"
     CDS             19454..21253
                     /locus_tag="U2R41_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEM8888658.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="U2R41_001236"
     CDS             complement(21321..22895)
                     /locus_tag="U2R41_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM8888659.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="U2R41_001237"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="U2R41_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEM8888660.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="U2R41_001238"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="U2R41_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEM8888661.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="U2R41_001239"
     CDS             complement(24672..25310)
                     /locus_tag="U2R41_001239"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEM8888662.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="U2R41_001240"
     CDS             complement(25311..25961)
                     /locus_tag="U2R41_001240"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM8888663.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="U2R41_001241"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="U2R41_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEM8888664.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="U2R41_001242"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="U2R41_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEM8888665.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="U2R41_001243"
     CDS             complement(29104..29844)
                     /locus_tag="U2R41_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEM8888666.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="U2R41_001244"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="U2R41_001244"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEM8888667.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="U2R41_001245"
     CDS             complement(31215..32153)
                     /locus_tag="U2R41_001245"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEM8888668.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="U2R41_001246"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="U2R41_001246"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM8888669.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="U2R41_001247"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="U2R41_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEM8888670.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="U2R41_001248"
     CDS             complement(35313..35618)
                     /locus_tag="U2R41_001248"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM8888671.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="U2R41_001249"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="U2R41_001249"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEM8888672.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DASHWH010000025        36230 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.025, whole genome shotgun sequence.
ACCESSION   DASHWH010000025 DASHWH010000000
VERSION     DASHWH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.025"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            232..1500
                     /locus_tag="U2R41_001250"
     CDS             232..1500
                     /locus_tag="U2R41_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEM8888673.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="U2R41_001251"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="U2R41_001251"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEM8888674.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="U2R41_001252"
     CDS             3076..3351
                     /locus_tag="U2R41_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEM8888675.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="U2R41_001253"
     CDS             3357..4124
                     /locus_tag="U2R41_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEM8888676.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="U2R41_001254"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="U2R41_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEM8888677.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="U2R41_001255"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="U2R41_001255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEM8888678.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="U2R41_001256"
     CDS             8268..8570
                     /locus_tag="U2R41_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEM8888679.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="U2R41_001257"
     CDS             8573..9736
                     /locus_tag="U2R41_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM8888680.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="U2R41_001258"
     CDS             9762..13190
                     /locus_tag="U2R41_001258"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEM8888681.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="U2R41_001259"
     CDS             13211..14335
                     /locus_tag="U2R41_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888682.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="U2R41_001260"
     CDS             14325..14762
                     /locus_tag="U2R41_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8888683.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="U2R41_001261"
     CDS             14746..15084
                     /locus_tag="U2R41_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEM8888684.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="U2R41_001262"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="U2R41_001262"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEM8888685.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="U2R41_001263"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="U2R41_001263"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEM8888686.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="U2R41_001264"
     CDS             16241..17284
                     /locus_tag="U2R41_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEM8888687.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="U2R41_001265"
     CDS             17443..18171
                     /locus_tag="U2R41_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEM8888688.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="U2R41_001266"
     CDS             18287..18532
                     /locus_tag="U2R41_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8888689.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="U2R41_001267"
     CDS             18529..18747
                     /locus_tag="U2R41_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEM8888690.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="U2R41_001268"
     CDS             18883..19422
                     /locus_tag="U2R41_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEM8888691.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="U2R41_001269"
     CDS             19415..21688
                     /locus_tag="U2R41_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEM8888692.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="U2R41_001270"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="U2R41_001270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEM8888693.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="U2R41_001271"
     CDS             complement(22861..23115)
                     /locus_tag="U2R41_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEM8888694.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="U2R41_001272"
     CDS             complement(23515..23784)
                     /locus_tag="U2R41_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888695.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="U2R41_001273"
     CDS             complement(24254..24709)
                     /locus_tag="U2R41_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEM8888696.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="U2R41_001274"
     CDS             complement(24847..26775)
                     /locus_tag="U2R41_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEM8888697.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="U2R41_001275"
     CDS             complement(26972..27805)
                     /locus_tag="U2R41_001275"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEM8888698.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="U2R41_001276"
     CDS             complement(27997..28701)
                     /locus_tag="U2R41_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8888699.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="U2R41_001277"
     CDS             complement(28724..29350)
                     /locus_tag="U2R41_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8888700.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="U2R41_001278"
     CDS             29676..32051
                     /locus_tag="U2R41_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEM8888701.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="U2R41_001279"
     CDS             complement(32075..32566)
                     /locus_tag="U2R41_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEM8888702.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="U2R41_001280"
     CDS             32753..33673
                     /locus_tag="U2R41_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8888703.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="U2R41_001281"
     CDS             33827..34171
                     /locus_tag="U2R41_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEM8888704.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..34861)
                     /locus_tag="U2R41_001282"
     CDS             complement(34361..34861)
                     /locus_tag="U2R41_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8888705.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35389..35472
                     /locus_tag="U2R41_001283"
     tRNA            35389..35472
                     /locus_tag="U2R41_001283"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35423..35425,aa:Leu,seq:cag)
     gene            complement(35796..>36230)
                     /locus_tag="U2R41_001284"
     CDS             complement(35796..>36230)
                     /locus_tag="U2R41_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEM8888706.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11989 a         6326 c         7492 g        10423 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaaggc gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgttgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc gtttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    34741 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    34801 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    34861 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    34921 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    34981 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    35041 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    35101 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    35161 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    35221 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    35281 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    35341 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    35401 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    35461 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    35521 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    35581 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    35641 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    35701 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    35761 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    35821 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    35881 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    35941 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    36001 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    36061 gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt
    36121 tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
    36181 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DASHWH010000026        34636 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.026, whole genome shotgun sequence.
ACCESSION   DASHWH010000026 DASHWH010000000
VERSION     DASHWH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.026"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            591..1280
                     /locus_tag="U2R41_001285"
     CDS             591..1280
                     /locus_tag="U2R41_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888707.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="U2R41_001286"
     CDS             1267..2475
                     /locus_tag="U2R41_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8888708.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="U2R41_001287"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="U2R41_001287"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEM8888709.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U2R41_001288"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U2R41_001288"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEM8888710.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="U2R41_001289"
     CDS             complement(6578..7273)
                     /locus_tag="U2R41_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEM8888711.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U2R41_001290"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U2R41_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEM8888712.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="U2R41_001291"
     CDS             complement(8472..8663)
                     /locus_tag="U2R41_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEM8888713.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="U2R41_001292"
     CDS             complement(8684..9574)
                     /locus_tag="U2R41_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEM8888714.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="U2R41_001293"
     CDS             complement(9561..10328)
                     /locus_tag="U2R41_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8888715.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U2R41_001294"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U2R41_001294"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEM8888716.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U2R41_001295"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U2R41_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEM8888717.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U2R41_001296"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U2R41_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEM8888718.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="U2R41_001297"
     CDS             14899..15282
                     /locus_tag="U2R41_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888719.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="U2R41_001298"
     CDS             complement(15450..16208)
                     /locus_tag="U2R41_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEM8888720.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="U2R41_001299"
     CDS             complement(16226..17044)
                     /locus_tag="U2R41_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM8888721.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U2R41_001300"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U2R41_001300"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEM8888722.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U2R41_001301"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U2R41_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEM8888723.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="U2R41_001302"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="U2R41_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEM8888724.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="U2R41_001303"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="U2R41_001303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEM8888725.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="U2R41_001304"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="U2R41_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEM8888726.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="U2R41_001305"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="U2R41_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEM8888727.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="U2R41_001306"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="U2R41_001306"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEM8888728.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="U2R41_001307"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="U2R41_001307"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEM8888729.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="U2R41_001308"
     CDS             27086..27385
                     /locus_tag="U2R41_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEM8888730.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="U2R41_001309"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="U2R41_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8888731.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="U2R41_001310"
     CDS             27973..28209
                     /locus_tag="U2R41_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEM8888732.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="U2R41_001311"
     CDS             28352..30325
                     /locus_tag="U2R41_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEM8888733.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="U2R41_001312"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="U2R41_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEM8888734.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="U2R41_001313"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="U2R41_001313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEM8888735.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="U2R41_001314"
     CDS             33204..34496
                     /locus_tag="U2R41_001314"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEM8888736.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DASHWH010000027        33274 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.027, whole genome shotgun sequence.
ACCESSION   DASHWH010000027 DASHWH010000000
VERSION     DASHWH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.027"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..1039)
                     /locus_tag="U2R41_001315"
     CDS             complement(20..1039)
                     /locus_tag="U2R41_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888737.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1061..2068)
                     /locus_tag="U2R41_001316"
     CDS             complement(1061..2068)
                     /locus_tag="U2R41_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8888738.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="U2R41_001317"
     CDS             complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="U2R41_001317"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM8888739.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2719..5013)
                     /locus_tag="U2R41_001318"
     CDS             complement(2719..5013)
                     /locus_tag="U2R41_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM8888740.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5273..7438
                     /locus_tag="U2R41_001319"
     CDS             5273..7438
                     /locus_tag="U2R41_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8888741.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7742..8575
                     /locus_tag="U2R41_001320"
     CDS             7742..8575
                     /locus_tag="U2R41_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEM8888742.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8677..10377)
                     /locus_tag="U2R41_001321"
     CDS             complement(8677..10377)
                     /locus_tag="U2R41_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888743.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10884..12044
                     /locus_tag="U2R41_001322"
     CDS             10884..12044
                     /locus_tag="U2R41_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8888744.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12060..12503
                     /locus_tag="U2R41_001323"
     CDS             12060..12503
                     /locus_tag="U2R41_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8888745.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12986..13516)
                     /locus_tag="U2R41_001324"
     CDS             complement(12986..13516)
                     /locus_tag="U2R41_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEM8888746.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13621..14172
                     /locus_tag="U2R41_001325"
     CDS             13621..14172
                     /locus_tag="U2R41_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8888747.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14325..14798)
                     /locus_tag="U2R41_001326"
     CDS             complement(14325..14798)
                     /locus_tag="U2R41_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8888748.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            14936..16408
                     /locus_tag="U2R41_001327"
     CDS             14936..16408
                     /locus_tag="U2R41_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8888749.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16405..17115
                     /locus_tag="U2R41_001328"
     CDS             16405..17115
                     /locus_tag="U2R41_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEM8888750.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17467..17751
                     /locus_tag="U2R41_001329"
     CDS             17467..17751
                     /locus_tag="U2R41_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEM8888751.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18032..18451)
                     /locus_tag="U2R41_001330"
     CDS             complement(18032..18451)
                     /locus_tag="U2R41_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEM8888752.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18453..19037)
                     /locus_tag="U2R41_001331"
     CDS             complement(18453..19037)
                     /locus_tag="U2R41_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEM8888753.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19084..20559)
                     /locus_tag="U2R41_001332"
                     /pseudo
     CDS             complement(19084..20559)
                     /locus_tag="U2R41_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            20740..21051
                     /locus_tag="U2R41_001333"
     CDS             20740..21051
                     /locus_tag="U2R41_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888754.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21166..23502)
                     /locus_tag="U2R41_001334"
     CDS             complement(21166..23502)
                     /locus_tag="U2R41_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888755.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23644..24312
                     /locus_tag="U2R41_001335"
     CDS             23644..24312
                     /locus_tag="U2R41_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888756.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24315..25358
                     /locus_tag="U2R41_001336"
     CDS             24315..25358
                     /locus_tag="U2R41_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8888757.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25595..26476)
                     /locus_tag="U2R41_001337"
     CDS             complement(25595..26476)
                     /locus_tag="U2R41_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8888758.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26635..27363
                     /locus_tag="U2R41_001338"
     CDS             26635..27363
                     /locus_tag="U2R41_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEM8888759.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27519..27836
                     /locus_tag="U2R41_001339"
     CDS             27519..27836
                     /locus_tag="U2R41_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8888760.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27829..28437
                     /locus_tag="U2R41_001340"
     CDS             27829..28437
                     /locus_tag="U2R41_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEM8888761.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28492..29283
                     /locus_tag="U2R41_001341"
     CDS             28492..29283
                     /locus_tag="U2R41_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEM8888762.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29421..29909
                     /locus_tag="U2R41_001342"
     CDS             29421..29909
                     /locus_tag="U2R41_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEM8888763.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29960..30832
                     /locus_tag="U2R41_001343"
     CDS             29960..30832
                     /locus_tag="U2R41_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM8888764.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31008..31370
                     /locus_tag="U2R41_001344"
     CDS             31008..31370
                     /locus_tag="U2R41_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8888765.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31379..32131
                     /locus_tag="U2R41_001345"
     CDS             31379..32131
                     /locus_tag="U2R41_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888766.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32145..>33274
                     /locus_tag="U2R41_001346"
     CDS             32145..>33274
                     /locus_tag="U2R41_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888767.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPP"
BASE COUNT        10339 a         5888 c         6844 g        10203 t
ORIGIN      
        1 tatttagtga gtcagctatt tagtatgggt tttctaaagg aacgactgtc tctcttttga
       61 tcaagcgatg agggatgacc aaacgtacgc cactgctcaa ttcttcatgg atcaattcgc
      121 gcagcttttg catagcgaca cgtcctaatt cggagatatc gatatcgata cttgtaaggt
      181 aagggtgtgt taatgttgag aagatcgagt tgttgaagct aatgacggag agtgtctctg
      241 gaatgacata gccatacatt tgcgccaact gcatcgtgcg aagggcgaag atatcgtcaa
      301 tgaccaccaa tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac agataatcct
      361 ccggatgctc tagtactaca ctggataacg caggaagacc agccatcatc attgcttttt
      421 gataaccgaa atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc acaaacagga
      481 tattcttatg tccattctgg atcaaaaatt ctgttgcttg tttacctaga agctggttat
      541 cattatctac ataaacgatt tcgttctcat gatgataagg ctgtccgatc aaagtgaaag
      601 ggatgtggtt ttggtataaa taatcaatga cagtatcttg actatctgaa tacactagaa
      661 taaaaccatc tacttgtttt tgtaaatgca tacgctggac gttctctaac agcaattcaa
      721 aatttttagc agtagcgatt gctgtagtca tatcataatt ccgcgcttct tcgttgattg
      781 cttccatgat ctcaagataa aatgggttac cgatccgctc ttttgaatca agagggggta
      841 agattacgcc gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc atgttaggaa
      901 cataacctag ttcatccata atttttcgga ctttcttctt tgttgtctcg gaaatactag
      961 gatgatcgtt gatgacacga gagacggtcg aggtggcaac accggctttt ttcgccacat
     1021 cttttacggt aatagccatg ataaaaacac ctttctcttg ttattgtgca gagaaccgat
     1081 aaatcgtctg actgatccgt tcttgaccag gatggaggtt gatcgatcca aatttgggga
     1141 aatgaacgat atcagggatt tcttgcggtt cgattgctaa tccgtagttt gaatgcatct
     1201 gttttccatt gacagagaaa tcagcttcga atccagtcgt agaaaataat accatacttt
     1261 tattagaagt tgctatagtc atttggcgac cagaatgctt ctcgaataat tgtaaggaag
     1321 ttttggaaag cctaggatga tgcaaaagaa atacatcatc tagtccatct ggataacatg
     1381 caaggatctc ttgcagtgtt ggggattttc taaagtcgat tggcagtttt tctttttttg
     1441 tccaatttcc agtaggcagt ttttcttgat ctaattcaag cacaccatct aaaaagaccg
     1501 ttaactgatg ttcatagatg tctcttgttc catttcctga aaggttgaag taggcatggt
     1561 tggtcggatt gcagatggtt tcaacaaggc tactagcaga ggttgtcatc tctaattcgt
     1621 tttttgttaa acggtaagtg atcgtagcag tgattggtcc tggataggta gaaaattcat
     1681 cttttaagta aaagaccaca cctattgatg atggagtttt gaaaacttct acttcccaaa
     1741 attggaaaga ccagcctttc gtgccaccgt ggatatggtg tgatccgcag tttttctcca
     1801 atctatgctc accccaagaa gcatcccgga tccttccggc tacaggtccg acagttgctc
     1861 caaaaaagct tttgtctgtt agaacatctt cgaaacgatc gtaagacaat aaaacatttt
     1921 ctgattttcc ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg taattgagaa
     1981 gaacgacttc taagtagtcg ttcttcaatg tgattttctt tatttcagtc tctggaactg
     2041 gtacgacgga gatctctgga ttatccatgg tcatgccact tttctttcaa gtaatcgatc
     2101 gtaagaaaag tcgtatcagg gacaagcggg atctcctctc caaggtcttc agcacgtccc
     2161 acgccaattg gttgagcgcc acttgagagg atggcttgga taccggcttg cgcgtcttcg
     2221 atcccgatac attcttctgc ttttagtccg acagcttttg ctgcaaggag gaagatatct
     2281 ggcgcaggtt ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa gtaaggtgta
     2341 agacccattt tttcaagtag gaatggcccg tttttacttg cagaagcaag agcaatcttg
     2401 attttttctt cttttaaaga atccagtaaa ggcacgaccc cggggaagac atcttttggt
     2461 tcgatcgtct ggatcatctc taaataataa tcgttttttc ttttcgctaa ttctgcaaat
     2521 tcttcctttg aaaaagagtg ttcttttccg ccataagcta aaagcaaaga gagtgagtct
     2581 tctcggctga cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat gccgatttcc
     2641 tgaccaagct ttcgccaagc acgataatgg aattcagcag tatcggtaat gacaccgtct
     2701 aaatcaaaca ataccccttt aaacatttgc ttcctccttt cttagcgtta tttctagacg
     2761 atccgctaaa gaataagact gttcatagac gtaaatttct agaggagaac cttctaataa
     2821 ctcgaaagta acttgttcat ctgtgactga gatgaacaat aaacgtccgc gataattgat
     2881 atggaaagaa taagctttcc aagtttttgg cagaaatggt gcgaatctca actgatcatg
     2941 ggcagttttc atttgagcaa atccttggac aatcgacaac cagctaccag tcatggaagt
     3001 gatgtgcaag ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc gagctgttcg
     3061 ctggtacatt tctacagctt tgctttccat tcctaattct gctgctaaaa tcgaatggat
     3121 acttggcgaa agggaagact catggacagt cattggttca tagaaatcaa agtttttttg
     3181 cttttcttct tttgtaaaag tatcattgaa gaaataaatc ccctgtagta catctgcctg
     3241 cttgatgaag caagatctaa ggattttgtc ccaagaccaa tgctgattca gaggaagatc
     3301 gcttgtggga atatcggata caggcatgag atctttatct aagaatgtgt catgctggac
     3361 aaagattcct aattcttcgt caactgggaa gtacatgttc tctgcgatat ctttccattt
     3421 ggctagttct tcttcgctga tagtgatagc agcttctttt tgataagtta aataattttc
     3481 tcgcgtgtaa cgcaagaccc aagctgcgat cgtatttgta taccaattgt tattgatatt
     3541 gttctcatat tcgtttgggc cggtcacacc gtggatcatg tatttatttg ctcgttttga
     3601 aaaatggaca cggtctgccc agaagcgtgc gatttctgct aaaacttcca gtccttcatt
     3661 tttcaaatat tccgtatcgc cggtgtaatt cgtataattg tagattgcat aggcaatcgc
     3721 gccatttcgg tgaatctcct cgaatgtgat ttcccactcg ttatggcatt cgacaccagt
     3781 gaaggtgacc ataggataaa gcgcgccttt taatccttgt tgctgagcgt tgtggattgc
     3841 ttgcggcagt tgattatgcc gatatttcag cagattctta gttacttcag gtttggctaa
     3901 agcaagataa agagggacag catacgcttc tgtatcccag taagtggctc cgccgtattt
     3961 ttctcctgtg aatcctttgg ggccgatatt caatcgttca tcttcgccgt agtaagtaga
     4021 gaatagttgg aacaaattga atcgaatacc ttgttgtgct tcttcgtctc cttcgatgac
     4081 cacatcggca agcttccatc gttctgccca agccgctgtt tgctcttctt ttgcttggat
     4141 ataattgttt gtctggttcg ttaattctga gacagctttt tctgcttgct gcgcttctgg
     4201 aatatctcta ctagtaagaa cgataatttc tttttccact gaccatgtct caccttgaga
     4261 taattctgca gacaagcaac catgtaatgc taaagtatct tcgtgatagt cagggatgac
     4321 tgattttcca ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt caatcccaaa
     4381 gttgtttgga atagttttag tcgtcaaata gccgatttct tctatagttc cacgatcaat
     4441 ctcattccaa aaatgttcat catagttgct gtcttcgttt tgaacatttc catccaattt
     4501 tgatacgagt ttgactgttc cgctaccttc aagcatttct atagttaaat agatataagc
     4561 tgattctttt ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt tattagtaga
     4621 agtagtgatt gtgaatgtac gagataatac accatttttc atatctaatt cccagtaaaa
     4681 gtcttctggt tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa tatctatcgc
     4741 gataaagttc aaggcgttga tcacttttcc aaagtattct ggataaccat ttttccacca
     4801 gccgacacgt gtcttatcgg gataccagac acccgccaaa taagtacctt gatggtggtc
     4861 ccctgagtaa gtttcttcaa agttcccgcg catccccata taaccgtttc caatacttgt
     4921 taaagattct tgcagtcgaa gatttttttt atctaattgt gtcgtgctga ttttccatgg
     4981 atcaatagca aatagtcgtt tgatctgttt catttttctc ctcctaatga gctgttttat
     5041 acgttcataa taaacgcaac cgattgcaca agtcaataga caaaagaaaa tatttcatag
     5101 aaaaaagctt aaaaaaataa atttttagga gaaaattata gaagggaaca cacgtattat
     5161 aggttttata gaatggtata gacagaaaaa taaaaaataa agcatttaca aaaaagaaaa
     5221 cgcacgctat aatacgttta acgaaaccga ttgcgtaaag gagctaaacg gaatgaaaaa
     5281 gatatttagt tttgagtttt ggcaaaaatt tggaaaggct ttaatggttg tggtagcagt
     5341 tatgccggca gctgggttga tgatcagtat agggaaatca attcccttga tcaatcctga
     5401 ttggacgccg cttgtcacaa cgggcggagt aattgaaaat attggttggg caattattgg
     5461 taatcttcat attttatttg ctttagcaat aggaggaagt tgggcaaaag aaagagcagg
     5521 cggagcattt gcagctggga tctcatttat tttaatcaat cggatcactg gggctatctt
     5581 tggtgtcaca agtgacatgc ttgcgaatga agatgcattt acccacactc tgttcggtac
     5641 gaagatcatg gttaaaggat tttttacaag tgtattggaa gcaccagctt tgaatatggg
     5701 agtattcgta gggatcatcg ctggatttgt cggggcaatg gcttataata agtactataa
     5761 ttatagaaaa ttgccggatg ctttgtcatt tttcaatgga aagcgctttg ttccgtttgt
     5821 tgttatctta tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc caaacgtaca
     5881 ggctggaatc aataattttg gtttatggat tgcagaatca caagaaagcg caccgattct
     5941 agcaccgttt ttatttggaa cattagaacg tttactattg ccatttggat tgcatcatat
     6001 gctgacgatt cctatcaact atacacagct tggcggatcg tatgagattt tatcaggcgc
     6061 acaggcgggg acacaagtat ttgggcaaga tccgttatgg ttggcttggg ccactgattt
     6121 agtgaatctt aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc ataactggac
     6181 gccggctcgt ttcaaagtag gacaaatgat tggttcttca ggtatcttga tgggaatggc
     6241 ttttgcgatg tatcgtaatg tcgatcctga taagaaagca agatataaat caatgtattt
     6301 ttcagcagcg ttggctgtct ttctaacagg tgtaacagaa ccattggagt tcatgtttat
     6361 gtttgctgct gtgccattat atgtcattta ttcagtgatc caaggggctg catttgcgat
     6421 ggcagatatc ttgccattgc gggtccattc atttgggaat atcgaattgc tgactcggac
     6481 accgttagct atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt tgtgtgtgat
     6541 cgcttttggt gttgttacgt atttcttagc aaatttcttg atcaaaaaat tcaactttgc
     6601 tacaccggga cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg tatctaacag
     6661 cggaacagga acagcagatc agcaagtggt ccagatcatt catttgctgg gaggaaaaga
     6721 aaatatcaaa gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa atgatcgaga
     6781 aaaagtaggg actgaagaag cctggaaacg agcaggggcg atgggattga tcgtcaaaga
     6841 caatggagtc caagcagttt atggaccaaa agcagatgtc ttgaaatcag atatcgaaga
     6901 tctgctgcaa tcaggagcag agattccaga gccagaaatg gtttctacag gaaatggaca
     6961 agcaggtagt aagcaatact taggtataga acaagaacta gtttccgcag cttctggaga
     7021 agtgattcct ttatctgaag taaaagatcc ggtattctca caaaaaatga tgggtgacgg
     7081 ctttgctgtt attccaacat caagagaagt tgttgcgccg atcgctggaa aagtgacaag
     7141 tattttccct agcaaacatg caattgggct ggaaacgaaa gatggtatcg aagttttgat
     7201 ccatatggga atcgatacag tccaaatgaa tcagccagct tttgagattg ctgtcaaaga
     7261 aggccaagaa gtaggaacag gaacgaaatt agctgagatg gatcttgatg tgatccaaaa
     7321 cgaagaaaaa gatccaacga tcatgatcgt atttacaaac gataaagtag aagaagtagt
     7381 gatcaagcaa ttaggtacag tgacagctgg taaagtgatc ggctccatta aaatgtaagg
     7441 agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca aaaatagctc
     7501 ctcctatttt tcgtttggtt gggcttatta ccgagaagca gcagtttgaa tactgtttag
     7561 tggagaggtc gtgaaaaagc tgccctgcac caagtgataa aaacagccaa acaaaaaata
     7621 gctcctggtc cattgagctt attactgagg agttagcagg agaacactat ttgctggaga
     7681 ggttgtgaca aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa ggagaaaacc
     7741 aatgaaattc atgacactca acacgcatag ctggcttgag ccagaaccag agaaaaaatt
     7801 gcaagaatta gcagataaga tcctgttaga agattatgaa gtgattgctt tacaagaaat
     7861 caaccaatta ctggaatctg aagaagtaga acctgcgaag ctaatgaagt tctgtccggt
     7921 aaagaatcaa gtacctatcc gtaaagataa ttttgcctac cgtttagttc agcttttaaa
     7981 ggaacacgga aaagagtatt tttggagttg ggaaatgagc cacatcggtt atgataaata
     8041 tgaagaagga aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc ttaccgtctc
     8101 ccaatttcaa aagaaagaag attatcagac acgcaagatc ttgattggga aaacaaagat
     8161 agatgatcag gacatttttg tctcgagctg ccacttttct tggtggacag ataaaaaaag
     8221 tggcttttat tttgagtgga aaaacttgga aaactacttt ttggaaactc gcgtaccact
     8281 ttttttccta ggtgatttca ataatccagt agactctcaa ggttattata ctgtaagaga
     8341 aagttgcctt cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa aagcagctac
     8401 tgtggaaaag aaaatagatg gttgggaaca aaatacagaa aaattgcgta tagattttat
     8461 tttcgtccct gaaggaatgc aggttaaaaa atatcaacgg atttttgatg ggatcgatag
     8521 tccaatcatc agtgatcact acggggtgga aatcgaatta gatgtgaacg aataaaaaat
     8581 ataacatata acataaaaaa actgattaaa ataaaactct ccaaacgatt ataccgtttg
     8641 gagagttttt tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa gcttgtatct
     8701 atgataaata aataaacata gcaaggtaaa ccagccaatc aaagtaagag aaagaatgaa
     8761 atagaaatag attggcgttt gataatgaag cgtgagctcg ttgattcctt tttgctgctt
     8821 cacgattggg gtcccgatat ctgttacttc ataatcagtc aatgtttttc cattttgctg
     8881 tagcacagtc cgatcgtaga cgataacagg gatatgaaca gttttgtccc cagtgttttt
     8941 ccacttgatt gtcaaaagac cgcctggatg tgtttttata aaagttgttt cgttttctag
     9001 tatttcttcg ttataccggt cgtatttatt gtcttttgtc tccttataga ttggcagata
     9061 gtcaggtgtg gttttttgga aaacgaacaa gctggtagac atatctttgc tgaagaatgt
     9121 tttccttgct tcttccggcg tatcgaatag ataagtatgt cgactgacaa atgtttcatt
     9181 ttcccacttt tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa aaataagaat
     9241 gccaaatata cttaaaagac ggaaccactt ttgttcagac cactggttca gtgaaagggc
     9301 aaggtaaagt agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa actggatcaa
     9361 actaataaaa ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc tagtagaaag
     9421 aaaaaagaaa acaagacttg tgtacaacac gattttttgg aataaggtgt tcttttgttg
     9481 aaagaaaata aaaagcaaaa aacctgataa tatcaatata ggaaaggcct tagggtaatg
     9541 gagccaataa ctgccatcca gatctatcgc cttttgactc atttctctgt taacaaaggg
     9601 aggaagaatc ttattcccac tgtaaacaat agctaatcct gcccaaatat ttgctgtcag
     9661 aacaaaataa agcaagaccg acttaatcag ttgaagcagt gcttttcctt tttcagtctg
     9721 gttaaaaaag taataaagat aaaaagggat atacatcaaa gctaacatga tcgaagacag
     9781 taaatggatc tgtgtcataa gtcccactga gacagcgact ttgataacag ggaacttttt
     9841 atgaaggacg aaatcagcaa tagggatcat acataaagga taaaaacaag tcccccagct
     9901 agtgaatcct tggttgatag accagtattg aatagaatac gtactcatga ataaaacggc
     9961 tactggtaca cttacccaat ttcgaacatt gacatatctc agtaaaatca gcatagaaaa
    10021 tcctgaaata agataaagaa tgaaatttga aagaatctga taatgaaacc agctacctgc
    10081 aagcaacacg atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg cattcacgat
    10141 cctgccactt tgctggaatc cataaaggga aggaaaataa tggaaatccc catatttaat
    10201 ttgttgcgcg gcatcataga accgattgta atggaatggg gcatcagcac ccaaaattag
    10261 tttatgtgtg aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa ttgctaaaaa
    10321 tgggccatat tttttcaatt gtcgaactcc tttgcaaact ttagataaat tcatcatata
    10381 tcctatgcga gaggtaggca agaaattaga aaattcatga aaatatttat gaattttaca
    10441 agaaaaacat aagtgaaagt tctaaggaga gaggatgcga cataagtttt gttgcatctc
    10501 cttgttttga aacagaattt ttcttaatac tcgaaacaaa gtagcttttt catgacctct
    10561 cggtaaacgg tgttcaatca gctgcttctc ggtaataagc tcactagcct agaaaatatg
    10621 agaagctatt ttctgactag cgctcttatt actcaaagca taacgcagtt ttcacaacct
    10681 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc caacaaaaaa
    10741 tatgagtagc tatttttttg ggctgtactt attacttgat gcaggacagc tttttcacaa
    10801 cctcatcggc tttcattttc tatttttttc ctgaacccat ataataaaag aaagaacaaa
    10861 aaaacttgca agggaggaca catgtgaagc agagtaagca aaaacagtct cgtcgacttt
    10921 tgtttcgttt gtttttatta atatgtatcg tcgtagggtt actgatcgct ttatatccat
    10981 tttatgtgga ttctttaaat agttttattg atcaaaaacg tatcgaacaa gtacaaaaac
    11041 ggaccgcagc tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaac caacgattaa
    11101 ctgaccaagg attcaatcct ggtactgatc ctttcaatga aaagaacaga aatgaaagta
    11161 caacatccag ccaattagag gaatggctga tcggaagtat caatatacct aagatccaat
    11221 taaatatttc actttatgat cggctcaatg gtaggatcct tgaaaatgga gcaggcgtct
    11281 tgcaaggaac ctctttcccg cttggtggaa attcgacgca ttcagtgatc tctgctcaca
    11341 gcggtttacc taatcgacgg ttatttaccg aattagatcg tttagaaaac ggagacacgt
    11401 ttattttgac tgttttgggg gagaaattgg cttatcaagt taaaaatata caagtggtac
    11461 tgccagatga cacgtctgtt ctaacgattt cagaaggaga agacttagtt accttgctta
    11521 catgtacgcc gtatatgatc aacacgcatc gtttgctagt gacaggacat cggattcctt
    11581 ataacgaatc ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc atgcggcaat
    11641 ggtttattct tgcaggaacg ataatagctg tagtcatact gttagtgttt atcggacgac
    11701 taatctatca gtatagatta agtaaaaaag tgcttgattt ttcatttatt attagtgatt
    11761 cgtcagggaa accggtaaat ggtagttcat tcattttgaa acataagaaa aaaacactta
    11821 ctagaaatgg tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc aagctggatc
    11881 aattgcctgg tggaacttat cgaatcgttt cagctgatga tcctaaagtt gctgtttctt
    11941 ttggtattag aaaagtaaaa caagagaaga tatacttttt tgaggggcga aaacttgtaa
    12001 aagagcttca aaaaaatggt ttttatttta aaatcaatga ataagtaagg agagaaaaaa
    12061 tgatccgcac aacaacaaaa ttgtctgcca aagaactgga atctgttcta tcgatttggc
    12121 tgcatgctaa cttggattct catgaattca ttccatcaac ttattgggag aatcaagtag
    12181 aatctgttag gaaagcttta ccagatgcag tgatttactt agcagaaata gatcaagaaa
    12241 ttattggttt tgctggggtg attgatcagt ttatcgcagg aatctttgtg aaaagggaat
    12301 accggcaaca aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc aataaaacac
    12361 tgactttgac agtctatcaa aaaaatcaat cagctattca tttctatctt tcacaaggtt
    12421 ttacaattca aacacagcaa aaagatgaag cagtaaatga ggaggaactt gtgatgaaat
    12481 ggaatcaaaa cgctgatttt tgatgaacag aataacgaaa taatgctccc agcctaattt
    12541 tttgctggga gcattatttc attttgaagt cactaattta actgtgtgaa tatgcagttg
    12601 ttacaatctt tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    12661 caaaatatga ggaactattt ttccatttgt ttctctaata ctcaggtcaa aacgctgatt
    12721 tcacaacctc ttgataaacg gtgttcaaac ggctgcttct cggtaataag cccactagcc
    12781 tagaaaatat gaaaagctat tttctaacta gcgttcttat tactcaaagc ataacgctga
    12841 tttcacaacc tcttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    12901 cggcaaaaca tgaggaacta tttttccgtt tctttctcta atactcaggt cagaacgctg
    12961 atttcacaac ctctctttca caaccttatt ttactcgttt ataattttca tcgaagtatt
    13021 ttccgctacg gatatcatct ggcattccat cgtatggttc ttcgctaata acatcttcat
    13081 tgtctatacc tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca agtaattttg
    13141 gataagtttc atatttttca cgagctgctt ccatttctgc gatatgttcg ttttggaaag
    13201 taacagtgat ttctggatgt tcttcaaccc atttagggaa aaagatcatt ccgtgaagtt
    13261 tcttttcatt tggcataaag tccaatgcca cgtctgtccc tgttggctct gtccaagtga
    13321 ttttgaatac gccgtccgtc aatttaacga tatcagcttc ttggtctttt acccaacggc
    13381 ctttgaccat tccgccatgg atacggtaat caacagtatg gtcatttttg gcataccatt
    13441 catattccca accattgtca tacgtataga taaagtgagt tccaataaaa tcttccaatt
    13501 ctttaaattc tttcatgttt aagcatctcc ttataaatta aaacatgtaa taaactacat
    13561 gttttcgatt actcatttat gatagaatgg atttaaagat ttgtcaagga gggaaaactg
    13621 atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa gaagaagatg
    13681 acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg gcaagcaaaa
    13741 catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat tcagcatcaa
    13801 gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga agaagggaag
    13861 gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa tcgagacgaa
    13921 tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt agaaattatt
    13981 cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg aaaaaaaacg
    14041 atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat tttagcgcat
    14101 gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga agaaagtaac
    14161 cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata tgaaagaaac
    14221 gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt atttatgtag
    14281 catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag ttcttttaac
    14341 aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa ttcgtctgtc
    14401 tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc gatttgtaaa
    14461 atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa aactttacgg
    14521 acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga caaagtaaat
    14581 ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa acgatatttt
    14641 ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc ccaatacctt
    14701 aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac aaccaattga
    14761 tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc ttttgtcctc
    14821 ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac aaatagcgaa
    14881 tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga gaaatatgag
    14941 taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt tagttgttgt
    15001 cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga cagcttttcc
    15061 tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa cgactggttt
    15121 cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata aattcagttc
    15181 aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta cttgctttac
    15241 agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg gtgtgggagt
    15301 atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa aaagaggatc
    15361 agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc ctacattatc
    15421 tggttatatc attgataact accattggcg tgacttgttt ggtatggtac tgccaatcgt
    15481 tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat tgtcaaatcc
    15541 tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt tactttacgg
    15601 attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt tcttagttgt
    15661 tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac atccattctt
    15721 ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag ctggtgtaac
    15781 aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa atattcgtgg
    15841 agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca tgggggctat
    15901 gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag ccatcacagg
    15961 gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa cgactccagt
    16021 tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg tcatgatgcc
    16081 ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg ggacagcggt
    16141 aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga tcagtgtgtt
    16201 gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata cgccattagc
    16261 ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt ttatcgtagc
    16321 gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa aagaacctgt
    16381 tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag cttgcttgct
    16441 tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc tttgattttc
    16501 ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa tcactttgga
    16561 atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga ttcccaagga
    16621 gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta tctttaccga
    16681 acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac aaataaagta
    16741 gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga atacaaaaac
    16801 gacatgtata aattctggtt caatattgct gataacaatc acgaatatga cagccgagtg
    16861 aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc agccaagtta
    16921 gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc ttatgttcaa
    16981 gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga gcaagaacaa
    17041 cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt agtgaaagaa
    17101 gcaaaaggcg aataatattt tgaacaccat gtaggaggtt gtgaaaaagc tgctctgcat
    17161 caagcagtaa gaacaatcag ataaaaatag cactccatat ttttcatctg attgggctta
    17221 gtgccgtaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta
    17281 actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg actattgtga
    17341 cttgtgaccg aggagttggc aggagaacac cgtttattcg aaaaaaggaa ctgcgacaag
    17401 acttttgtca cagttccttc ttttttttgc tatgctatac tagatgtaaa aaataggaga
    17461 aataaaatga gggaaattta ttcttttgaa tcaaaaatct atgcttccga agtcggcaag
    17521 ggcggggcat atattatttt tccgtatgat ataagaaaag aattcggtgc tggaagagtt
    17581 aaagtccaag caacttttga caatgtgccg tatgaaggca gtatcgtgaa tatgggcgtg
    17641 aaaaatccag atggttcagt ctgctacatc cttggactac gaaaagatat tcgtaaaaac
    17701 atcggaaaag aaatcggtga tattgtagca gttacagtga agcaaaaata aaagaggttg
    17761 tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc aaaagccagt ttctcggtag
    17821 taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc ttactactcg
    17881 aaacgaagcg gctttatcac aacctcttga taaacggcgt tcaatcagct gtccctcggt
    17941 attagctcaa aaacggcaaa atatgagaag ctatttttcc atttttttct ctcatactca
    18001 ggtcataacg ctgatttcac aacctcttct ttcataatgg tttttcacca tctttataat
    18061 aaactggttc tttgatatgg aaatctattt tttccaacca atctaaaatc ttgtaaccta
    18121 actcttctga catcggaaaa gaggcgttga tttgtgcatt ggagatatgg tcaacatgga
    18181 taccggaagt gatcgtacaa cttcccggaa cggcagtttt gatgatcaca gctatttttt
    18241 cggctaagac atcgtcttta tggaaccgac catcgtggct agggaaacgt acagtttgca
    18301 tctctgtatc acgagaaaat gtagtgactg ttccgatatg tggtgtatct ccgcctgtta
    18361 aagtgatcaa taagtcttta ccgattactt taacagcagc ttggatcgtg aaacctagtt
    18421 tggttacttc aactatcgtt tctttttcca tattaatcac cctgccaacg ttttgaatgc
    18481 tcatttacaa tttcaaattg atccaaaagt ttcattgtat ggtgattaac aagatcttgg
    18541 atcgtttgag gctgattata aaaagcagga attggtggta tgatttgcac acctagtcgt
    18601 gctaatttag tcaagttttc caagtgaatg gcatttaatg gcgtttcacg aggaacaatg
    18661 actaactttc gttgctcctt cattgttaca tcagcagcac gtgcaatcag gttgtcgcct
    18721 aaaccaacag agatacctgc gactgttttc atacttgccg gtacgatgat cattccatcg
    18781 gtcaaaaatg atccgctggc gacagcagca cctaaatctt tgtttgaata atggtaatca
    18841 aacaggcttt cgaattcagt tagtgataag tcggattcca gctttaagtt ctgttttgcc
    18901 catggactga tgattccgtg tgtttctatc atcggatatt cttttagttt cttcactagg
    18961 ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt tttcttccgt
    19021 tgtgccaatt gttccatttt ccgcactcct ttttatgatg tcaaaacagt tgcagatcat
    19081 tcattatttc aaatattttt cccaatcttc tacttcttgg aattttgcac gtacaaaatg
    19141 ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactta tcccacggaa
    19201 acgaatagag ccaggattat attctggtaa ttctgatggg tccaatggat ggttcctcat
    19261 tcctgggatc acgatgatat ctttatctcc ttggaaacga gtgttgaaag tccaaactac
    19321 atcgttcatg tcgaaaatat caacatcttc atcgactaaa atgactgtct tcaattcttt
    19381 gaaggctgat aaagcaagaa tagctgcttg acgctgaata ccttcatccg cttcgttttc
    19441 tttacggatt tgcatgatgg tcatcaattt accaccacct gcaggaggat tgtagacatt
    19501 tactactttt cctggaatag ctttatcgac caaggcaaga atacttgctt ctgtaggaat
    19561 accggccata gatacgtgtt cttcactggg accaatcgtt gtttgcatga ttggattgtc
    19621 ttttcggtga gtaactgctt tgactttcac tacttgtaca gctggattag catccccagt
    19681 atagccagga aattctggca ttgcttttcc tgagttcgtg ttgatatctt cttgcattgt
    19741 ttcatttggc atgatttctg cttcaatgat gaattcagca cgagcaatac ccacttcatc
    19801 gactgctata ccatcaacga tttgaatagg ttcgttacgc aaagctcctg cacaccatag
    19861 ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg caattgctgg
    19921 atctaaacca atattgatag tgataggcat tggtttattc aaggcttcga attgttttta
    19981 gaagtgacca atgtggcgtc cgccaggcat gatgtacata ccgatcgtat ctttatcttc
    20041 tagaaccata cgatggatcg ttacatcgct cattgttttt tctggatctg atcctaagac
    20101 tacacccatt gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg caggcaaaat
    20161 gttgcgaata tcgaagtctg catcggtagc tagatgaacg acttcttgtg ctggagcgtc
    20221 tgctttgtct actttcacag gtttgattgg attttctaca gcgtctttca agaaacgacc
    20281 aagtgttcga taatcatggt gcaaaatttc acctacacgt ttacgactag ccattgttcc
    20341 gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag ctggtccttc
    20401 ttgagtcgga cgttttactg taccgccagc accgatataa cggtaaacac cagataattc
    20461 tgcttcagga tcgatttcaa tatctgtttc gtgatattga cctggaagtg tttttagttc
    20521 ttcgatgact tttcttaaat catacggttg atctgtcatg agagattcct ccttagttgt
    20581 ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg ttcggtaaag
    20641 tattctacaa tggaatggat gcgttttttt gataatagaa taaactatga tatacttatc
    20701 actaaaagaa agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat ggaggagtca
    20761 tttcctaaag cagtaaaagt ggaaaatata gcgaatattt tgaaagttac atttgaaaac
    20821 ggagaagtaa aatacgtgaa atctcattgg acagaagaga taaccgatgc cttgcaattt
    20881 ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa tatgtggatt
    20941 gggactgaag taacgattga agcagatgga acagtattca taaatggaaa agacaggtat
    21001 acgccagaag agttgtggta caaaggaaag aaaagtatac cagaattata aaaaataatt
    21061 aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg tttcttgctg ttttctacta
    21121 tttcagcgct cttatgactc aaatctaaat gtggttgtca caaccttatt ctgcacgcaa
    21181 agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc caagcatcgt
    21241 caatatagta gagacgatca ctagaataat cgcatggaca ggatttagtt gagcaacatt
    21301 ttttagatcc gtcatgttgt ataagatgct attgataggg aacgtagcta gccatgcaat
    21361 aaacacacct aatatcccag aagaaatacc taaaatacac gtttctgcgt caaatacgcg
    21421 agtgatgtct tttttacgtg cacctaaagc ttttagtaca ccgatttctt ttgtccgttc
    21481 aatcacagat gtataagtaa tgatactgat catgatcata cttgttacta aagagatccc
    21541 tgcaaaagca ataaggacat aagtgatagc atccatcaat ccgccagtca gttcagtcat
    21601 cgttccagct aaatcagtat aaatgatttt atcttcatct gatttgcctt tattgtaatc
    21661 atctagataa tccaagatct tttctttgtc ttcaaaattg ttcggataaa tcatgatact
    21721 tgaaggcaag ctgtctccac ctaaataact aagcaatgtt tgtttggcat tttcgtcaac
    21781 tttttcagta gttaagacat ttacaccact gtctctttga gctttgacga tttctgattc
    21841 tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta taagcgattc ctggagataa
    21901 gagattcatt gtagaagaag atttcacgcg taggatcccc gagatcttca attcatcaga
    21961 agcattctga tacatcgcat cataatctgt attagggata aaattcccag ttggaagttt
    22021 tgtatagtaa gtattgttgt ttactaattt gaatgttgta ccaacgatat catcaaaatc
    22081 aagtttttcg tcttctttca catcaaatcc aagattcttc aatgcgttga tattagtatt
    22141 attattgccg tctacgatca gcacgacatc tgttgctgag gctggataac ttccggcaag
    22201 taaagaataa ttgtctttta aaaagttttc tttgctagtg tcaagctgtg tagggaaact
    22261 tgacactccc acacctgtca ttgctgacat cgtactagat aaagaaagag attccgtatc
    22321 aggattttga ttcgagaagc tgacaggctg tactttgcca tttacatcac gtaaaagatt
    22381 gattcctgta gtacgggtaa atccgatatt attacttaag ttaggatcga tgtctgttac
    22441 gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt gctttatctt cttcgctgac
    22501 ttttgcagta atcgttttgc tgtctggata atccgctttg cttgcaccca atccggcatc
    22561 gtcacgagtc tggttagtcg ttaccttaga aatagtaatc ggaaatttcg ccatcgtttc
    22621 agcttgcgtg ttatcgattt gtttttggaa tccagaagaa agggacagaa cgatcgcgat
    22681 tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt tttttgttcg
    22741 gatattgttg aaggaaagct ttaacgctgt ccaaaaactc atctttgttc tgcgtaagtt
    22801 aaattgatca tcttttggtc gttcgatgtg aggatgggaa tctgttaaga tcttaccatc
    22861 agaaaattca atgattcgat ctgcgtattg atgggccagt tcaggattat gtgtgaccat
    22921 gatcactaat ttttctttcg ataactcttg gatcaattcc atgatctgga tacttgtttc
    22981 tgtatctaac gctccggttg gttcatcgca caaaaggata tctgggtcat tggctagtgc
    23041 tcgagcgatg gccactcgct gcatttgtcc accagataac tgatttggct ttttatgcat
    23101 atgatttgtc aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt tttcgtcttt
    23161 agaaacacca cttaatgtca tcccaagttc aacgttttca ataatgccta gatgaccaat
    23221 caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat cccagtcact
    23281 gtccttaaag tctttagtag actttccgtt gattaccata tcaccggaat cataattatc
    23341 tagtccacca atcacattca gcatagtcgt tttaccagaa ccactaggac ctaaaatcgc
    23401 tacgaattct ttttgtctga aagcgacaga cacaccatcc aatgctttgg tggtggtttc
    23461 gccgacttta taatattttt ttatatcttt taattgaagc ataatcaccc ttctcccgta
    23521 ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa
    23581 tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga
    23641 tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct
    23701 gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa
    23761 aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat
    23821 ggctatgact tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt
    23881 acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat
    23941 atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt
    24001 tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct
    24061 tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg
    24121 tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt
    24181 tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg
    24241 acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact aggctataag
    24301 gcggttattt aaagatgaaa aaaaagctat tttcacaatt atggatctat tttgctagta
    24361 ttgtcttcat ttcgatctta gtgacactgc tgtgttttct cggttttatt ttcttattgt
    24421 ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc aacgttatac ccgttattcg
    24481 tctttgcggg gttcagtatg gtcgtgggta caggaatatc gatttttgtt gggcggcgta
    24541 tcctgcatcc aatcagtgcg ctagggacaa atatgagtct ggtagcaacg ggagattttt
    24601 cgattcgaat ggatgaacag cagaaagtag cagaagtaca gcaactgtat aaagatttca
    24661 acgttatggt ccaagaactc aatagtatcg agacactgag gaatgacttt gtgtccagtg
    24721 tatcccatga attcaaaaca ccactagcaa caatccaagg atatgttcaa ttgttacaag
    24781 ccccaaatct ctctgacgaa gaacgccaaa tatttttata tcggattatc gaaagtatca
    24841 cgcagctttc acaattaaca gagaacatct taaaattgaa caaactggaa aaccagcgta
    24901 tccaactgga aaagaaagag tatcgattag atgaacaaat cagagaagta atcgtttttt
    24961 ttcagcctaa atgggagaaa gaacagctag aattggatat cgaacttgct gcagtaaatt
    25021 acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa tatcatggat aacgccataa
    25081 agtacaatca aatcaatgga cagattcata tcaaactatt tgagacagcc acagagattg
    25141 tactggaagt gaccgattca ggtgtaggaa tgaatgaaga gaccagagat cgaatgtttg
    25201 aaaaatttta tcaaggagat acaagtcgtc aaatttctgg gaatgggcta ggactttcac
    25261 tggttaaaaa aattctagaa ctgcatgatg gacgtattga ttatagcagt atcgaaggag
    25321 taggaacgac agcaatgata cggttaagta aacaatagga aaaagaataa aacccctaaa
    25381 aattctattg aaggttttta ggggttttta gtcgtttttt tgtgattagg gaaatgttct
    25441 tacggttctt ttggcataaa aatcgctgct ttcttattga actacgagaa atgttagagg
    25501 tgtcattgaa acaaggacat gatgcaagaa agagagcgaa agtcatgcgt cgctctcttt
    25561 ttttgggtgg atagatggac agaaaatgta gggcttagtt ttcttcttga tttaaatact
    25621 cccaaaatgt atctgaagcc aaagaatgct caccgacttt acgagtaaat gcaagataac
    25681 tctctgttgt tggagaaata gggagaatca cagtattttc ttttaataga ttcttgactg
    25741 ttttttccat aacaatagca atccccatgt catttgcaat catattgatg atcaagtcga
    25801 ttcttgctcc tttataagag atttttggtt caaatccagc ttcgcgacac agatctagta
    25861 caggttgata caagttcgta gtggggccaa ggagcaaaaa gttctccttt ttcagttcgc
    25921 ttagatccaa tttcttttta ctagctaaag gatgattttt aggtagaact gctacaaaat
    25981 cgtctgtttc agtaggcaac cactcaatcg aacttggtgg ggcctggaaa cttctagcaa
    26041 agtggatcgt gtcactatgt tcattggcgt cattgtaagt taattcgaca ctttccactt
    26101 ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa ctcggtgatt ttggaaaaac
    26161 ctttataatt gatcattgtc ggtatcgtta agatatttaa tgaaagattg ttgtctttcg
    26221 tataggtttc aagcacttct tgcatagcat ggtattgttc tacgacattt tttacatatg
    26281 ggagcaataa gcttccagct tcggtcaatt cgatttttct atgggcgcgt tcgaataatt
    26341 tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga aatatttcct tgggtggtga
    26401 attgtcgatc agcagttttg gtgaaattca ttgtttcggc taaatcaaga aaaacttcga
    26461 atttatggat atccatcata ttcctcctac ttattccaat aaagaatact ttaacacaaa
    26521 aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt cttgcagcga aagcaataat
    26581 tacagaaatt tttctgattt ttttcgcttc gtagaaaaag agaggagttt tttgatgcca
    26641 actggagttt tgatcaatgt aggttccgtt ttgtttggcg gactaattgg cggattagta
    26701 ggaaataaat taagtgaaca ttttaaagca cagctgacga tggtttttgg tgtgtgttcg
    26761 atggggatgg gaatctattc cattgcgccg atgaaaaata tgccggctgt tatttttgct
    26821 ttagtgatag gaacagcgat tggtttgatt gttcatttag gtaatgggat aaataagggg
    26881 gctgcgctga tgcaagtacc tatttctaaa atcttcccta gtgaaaagct tggtatgacc
    26941 cacgatgaat ttatcagcac attagtgacg gtcattgttc tattttgtgc aagtggtacg
    27001 gggatctatg gaagtttaga ttctgggatg acaggagaca gtacgatttt gatttctaaa
    27061 tctgtccttg attttttcac agcagctatt tttgcgtgta atttagggta tgtcgtttct
    27121 gtgatagcaa ttccccaatt cattattttt tatattttgt tcttattggc aaaatttatt
    27181 tatccattga ctacaccaga catgatctta gattttaaag cttgcggcgg attcttgatg
    27241 gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc cagtagctga catgatacct
    27301 gcaatgatcg ttatcatgcc actgagctgg atgtggacaa attggattat gccactgtta
    27361 taataaaaat caaagagaag ccattttttg ctgactaaaa aggatcaaat cttttagtca
    27421 gtttttttgt atttgacaaa ctgattttga aactatatta tatgttgtat aatatagatt
    27481 gaaataaaat attacacttg agaatagaag gtgacgtaat gcacatccag attccaactg
    27541 ttcttcttga tggtactgtg ctagcagcat taagcaagga agatatgtat gggtatgcct
    27601 taacaaaaat cgtacaagaa agtctctctg tcagtgaatc tacactatat ccggttttga
    27661 gaagattgaa aaaagaagga tatcttgaga cctatgatga accttttgaa ggcagaaacc
    27721 gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt attgatgaat
    27781 ggattgtttt tcgagattct ttagaccggt tattggagga gagcacaaat gaatgaatat
    27841 ttgaatgaag ttgtggatca tcttcaagaa attcctgaag acgatcgttt tgatttgatc
    27901 cagtattatg aggaatattt tttagattct ggaaaaacat tagatcagat cattgatgaa
    27961 tatggtacac caaagcagtt tgccttaaaa ttaaaaatca gttacttttc agaagaagaa
    28021 caagcagaaa acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat ttggttaatc
    28081 attatcggtt tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt
    28141 attgggggac tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc
    28201 agtatcttag gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg
    28261 attctgagtg gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt
    28321 atttcgccac tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt
    28381 aaatggatgg gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg
    28441 aggacacata aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact
    28501 ggtggcctcc tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat
    28561 aatggtcctc ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa
    28621 atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata
    28681 aaaacgactt atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact
    28741 gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa
    28801 atcactattg ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat
    28861 agtgcaattg atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc
    28921 tgggtatcgt tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca
    28981 cttcgattag atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc
    29041 catctagaag acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta
    29101 tctttgcatg agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt
    29161 gaaaaagact accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag
    29221 atcactgcta aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat
    29281 tgatcctgta ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc
    29341 tgtttttcat cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag
    29401 aaaacagggt gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag
    29461 aaatggaaag attacatact tatatcactc aactaagcca aaccttctat gatccagaca
    29521 aagaaaaagt gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt
    29581 accatgaagt tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac
    29641 cgattatcga tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat
    29701 ctgttcggga agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact
    29761 attttacaga agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg
    29821 gagggacaca ggcaagcggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg
    29881 taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat tagatattta ttgaaggata
    29941 aaggagcgca ggaagtagca tgagagaaaa aagagcattt tatatcaatg gttatgcagg
    30001 gattgtcgga ttggtcgtat tagctttagt tggattgttt ttcttctatt taggtatgtg
    30061 gcaagcgaaa gtttgggcac tttttttaag tatttttctt tggttgatca cgttattgtt
    30121 gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt tttttggaca
    30181 atatttaggt acgattcgag aaaatggctt tttccttacg atacctttgg cacaaaaaat
    30241 gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca atgatttgga
    30301 tggaaacccc atcgaaatct cagcagtagt agtctttaaa gtcgttgata cagcaaaggc
    30361 attattcgat gtagcgtatt atcaagattt tgtagaaatc caaagtgaga cagccattcg
    30421 gcatatcgct agccaatacc cgtatgatac attcaatgag aatgatttga cgcttcgagg
    30481 aaacacgacg gcagtgtctg acgagttgca aaaagagctg caagagcgtt tagcagtagc
    30541 aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag aaatcgctag
    30601 tgccatgctc caacgtcaac aagcaaaagc cattttgtct gctcgacaaa ccattgttga
    30661 aggagctgtc acaatcactc aaatggcctt agagcaaatc gaagatggac aagaaatcaa
    30721 tttcaccgat gatcggaaag tccaattgat caataattta cttgtctcaa tcattacaga
    30781 caaaggaacg caacctgtta tcaatacagg cgacatgaaa gaccgtaagt gacatacttt
    30841 ttgtaagata ggaagaagga gtttaggacg gattattgtc ttgaactctt tttttagttg
    30901 ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg cagaattgtt
    30961 cgcttttttc tataatcagt atataaagta agagagaagg ggaaaaagtg aaaaaacttc
    31021 tgatcggtct acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc gcagattacg
    31081 tagttatggg tggagaagaa tactatgtac aaattacgac agatggtaca aaaacagttt
    31141 caaaatcaga ctctggacaa gaaacagtca actatagata tatcctgcct ggctatacga
    31201 aagagggtaa ggaaaaagaa ctggacttca agggacaatt gactcgaccg cttcgtaaag
    31261 gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat gaagaagtcg
    31321 aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat taaagggtag ggaaagaaat
    31381 gaacaaaata gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca atgagaaaaa
    31441 gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta ttgggatcat
    31501 gggcgcttct ggttctggta agtcgacact tttgaatatt ttatccacat tagataaacc
    31561 aacagatggg catatccgaa tcaatcaaaa agatgtgacc acattaaaag gaaatcaact
    31621 agcagatttt cgtgcgaatg agattggctt tattttccaa gatttcaatt tattagaaag
    31681 cttgacagca caagaaaata tcgctgttcc tctttcttta caaggcgtca gaccaaaaga
    31741 aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc atctctcata ttttggaaag
    31801 ctatccttct gagatttccg gtggacaaaa acaacgggtc gccgccgcac gtgcattgat
    31861 cacacaacca acgattctat taggggatga gcctacaggt gcgctggatt ccaaaagtgc
    31921 ccgggatctt ttagatacga tggacgaatt gaatacaaaa gatcatgtat cgattcttct
    31981 tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt attttattca tcaaggatgt
    32041 cggtatccat caagaagtga aacgtggaga tcaaagccgt gattccttct atcgagaaat
    32101 actatctatt cttggaaact tggaacagta gggggagatt gactatgctt tggaaattat
    32161 cacttactgg gatcaagagc cgtttaagag actacttggt gttattctca ggattagtta
    32221 tggcatctgc tattttttac atgtttgaat caatggcaag caacgaagct tttttgaaaa
    32281 gtaatacgat cgttgcggct acagttatca ttttccgatt cggttctgtt ttactaggga
    32341 tcatcacatt cgtttatatt ttatatgcaa attccttttt gatgacgatg cggcaaaaag
    32401 actatgcaat gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag atgatttttt
    32461 ctgaaacatt cttagtaggg atcgcagcta ctttagtggg ttcagctgtc ggtgtaggat
    32521 tgacaagtat cgtccaccga ttattagtgg atcagttaga tctcaatatt tcgcatttta
    32581 caccttttag tattcacggg ttgttgatca ctgttttgtt ttttgctgtt ttgtttttat
    32641 tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc gattttagca ttgattcgtg
    32701 cggatcagac accaacaaga atcaagcagc ataaattctt gtttttcctt gaagtagtac
    32761 tgggaattgc ttttctaggc tgcggttact atatgatgaa acacgtgggg acctatcaag
    32821 tctttggtat aggggtagcc ttagttacga tcgtattagg aacttatttc attttccatt
    32881 ctgtgatcat tttcttttta tcattactaa agaaatcaga acagatttca ttgaaaaaat
    32941 tgaacaattt cacattgtca cagttaaact ttcgtattcg tgaatataca caaatgctct
    33001 caatggtcgc tatgctgttt gccttggcac tgggcgcttt gacggtagga ttaggtttta
    33061 aaaatgaaat tgcagacttg acagaaaaca attctggata tgatctagtg atgaataatc
    33121 ctcgggcaga agatcagcaa aaaatcaaag aactttcacc aacactttct gcttcttata
    33181 ctcaaaaaga agatgcggga acgatttact ataatgcaag tgaatttgcg gctcaaccat
    33241 tgatcatgat aaaacatgaa gatggaacac cgcc
//
LOCUS       DASHWH010000028        31011 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.028, whole genome shotgun sequence.
ACCESSION   DASHWH010000028 DASHWH010000000
VERSION     DASHWH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.028"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U2R41_001347"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U2R41_001347"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEM8888768.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="U2R41_001348"
     CDS             1710..2027
                     /locus_tag="U2R41_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM8888769.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2231..2596)
                     /locus_tag="U2R41_001349"
     CDS             complement(2231..2596)
                     /locus_tag="U2R41_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888770.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2804..3286
                     /locus_tag="U2R41_001350"
     CDS             2804..3286
                     /locus_tag="U2R41_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888771.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3483..4373
                     /locus_tag="U2R41_001351"
     CDS             3483..4373
                     /locus_tag="U2R41_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEM8888772.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4698..7169
                     /locus_tag="U2R41_001352"
     CDS             4698..7169
                     /locus_tag="U2R41_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8888773.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7272..9008)
                     /locus_tag="U2R41_001353"
     CDS             complement(7272..9008)
                     /locus_tag="U2R41_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEM8888774.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9005..9709)
                     /locus_tag="U2R41_001354"
     CDS             complement(9005..9709)
                     /locus_tag="U2R41_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888775.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9840..10343
                     /locus_tag="U2R41_001355"
     CDS             9840..10343
                     /locus_tag="U2R41_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEM8888776.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10303..10641
                     /locus_tag="U2R41_001356"
     CDS             10303..10641
                     /locus_tag="U2R41_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEM8888777.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10662..12080
                     /gene="ffh"
                     /locus_tag="U2R41_001357"
     CDS             10662..12080
                     /gene="ffh"
                     /locus_tag="U2R41_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEM8888778.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12192..12770
                     /locus_tag="U2R41_001358"
     CDS             12192..12770
                     /locus_tag="U2R41_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEM8888779.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12774..13295
                     /locus_tag="U2R41_001359"
     CDS             12774..13295
                     /locus_tag="U2R41_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8888780.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13374..13928)
                     /locus_tag="U2R41_001360"
     CDS             complement(13374..13928)
                     /locus_tag="U2R41_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8888781.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14181..14456
                     /gene="rpsP"
                     /locus_tag="U2R41_001361"
     CDS             14181..14456
                     /gene="rpsP"
                     /locus_tag="U2R41_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEM8888782.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14468..14719
                     /locus_tag="U2R41_001362"
     CDS             14468..14719
                     /locus_tag="U2R41_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEM8888783.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14844..15635
                     /locus_tag="U2R41_001363"
     CDS             14844..15635
                     /locus_tag="U2R41_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888784.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15805..16326
                     /gene="rimM"
                     /locus_tag="U2R41_001364"
     CDS             15805..16326
                     /gene="rimM"
                     /locus_tag="U2R41_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEM8888785.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16316..17077
                     /gene="trmD"
                     /locus_tag="U2R41_001365"
     CDS             16316..17077
                     /gene="trmD"
                     /locus_tag="U2R41_001365"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEM8888786.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17213..17560
                     /gene="rplS"
                     /locus_tag="U2R41_001366"
     CDS             17213..17560
                     /gene="rplS"
                     /locus_tag="U2R41_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEM8888787.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17692..18828)
                     /locus_tag="U2R41_001367"
     CDS             complement(17692..18828)
                     /locus_tag="U2R41_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8888788.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(18944..19510)
                     /locus_tag="U2R41_001368"
     CDS             complement(18944..19510)
                     /locus_tag="U2R41_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEM8888789.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(19646..20299)
                     /locus_tag="U2R41_001369"
     CDS             complement(19646..20299)
                     /locus_tag="U2R41_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888790.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(20349..20777)
                     /locus_tag="U2R41_001370"
     CDS             complement(20349..20777)
                     /locus_tag="U2R41_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM8888791.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(20782..21156)
                     /locus_tag="U2R41_001371"
     CDS             complement(20782..21156)
                     /locus_tag="U2R41_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888792.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            21460..21645
                     /locus_tag="U2R41_001372"
     CDS             21460..21645
                     /locus_tag="U2R41_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888793.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            21658..21906
                     /locus_tag="U2R41_001373"
     CDS             21658..21906
                     /locus_tag="U2R41_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888794.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            21937..22107
                     /locus_tag="U2R41_001374"
     CDS             21937..22107
                     /locus_tag="U2R41_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888795.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(22209..22667)
                     /locus_tag="U2R41_001375"
     CDS             complement(22209..22667)
                     /locus_tag="U2R41_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888796.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            22835..23152
                     /locus_tag="U2R41_001376"
     CDS             22835..23152
                     /locus_tag="U2R41_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888797.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            23085..23564
                     /locus_tag="U2R41_001377"
     CDS             23085..23564
                     /locus_tag="U2R41_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEM8888798.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            23581..23757
                     /locus_tag="U2R41_001378"
     CDS             23581..23757
                     /locus_tag="U2R41_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888799.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            24014..24445
                     /locus_tag="U2R41_001379"
     CDS             24014..24445
                     /locus_tag="U2R41_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888800.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREIEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            24451..24768
                     /locus_tag="U2R41_001380"
     CDS             24451..24768
                     /locus_tag="U2R41_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEM8888801.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            24887..25573
                     /locus_tag="U2R41_001381"
     CDS             24887..25573
                     /locus_tag="U2R41_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8888802.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILYGIVRLPE"
     gene            25548..26897
                     /locus_tag="U2R41_001382"
     CDS             25548..26897
                     /locus_tag="U2R41_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8888803.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            26888..27361
                     /locus_tag="U2R41_001383"
     CDS             26888..27361
                     /locus_tag="U2R41_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8888804.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            27336..27863
                     /locus_tag="U2R41_001384"
     CDS             27336..27863
                     /locus_tag="U2R41_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEM8888805.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            27878..30190
                     /locus_tag="U2R41_001385"
     CDS             27878..30190
                     /locus_tag="U2R41_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM8888806.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTGEQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            30437..30754
                     /locus_tag="U2R41_001386"
     CDS             30437..30754
                     /locus_tag="U2R41_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEM8888807.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            30751..30912
                     /locus_tag="U2R41_001387"
     CDS             30751..30912
                     /locus_tag="U2R41_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM8888808.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDVHIENL
                     RREIRRLEE"
BASE COUNT        10373 a         5317 c         6258 g         9063 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga gggaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
     2101 atgctgatat ttcagcattt tttaagagta taaaagagaa aaaaagtaat aaatgtatac
     2161 atgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata
     2221 tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc
     2281 caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta
     2341 aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag
     2401 tagatataat attggcaatg aataaagata taatacgcct gttcctttga aaataatatc
     2461 cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat
     2521 caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc
     2581 agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat
     2641 tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa
     2701 actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa
     2761 ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt
     2821 aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg
     2881 ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt
     2941 ggtttctagg aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt
     3001 attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt
     3061 agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag
     3121 cgagagtgga ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg
     3181 gttctattta gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat
     3241 caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag
     3301 taaaaaagca tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg
     3361 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
     3421 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
     3481 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
     3541 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
     3601 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
     3661 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
     3721 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
     3781 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
     3841 tggcgccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
     3901 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
     3961 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
     4021 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
     4081 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
     4141 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
     4201 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
     4261 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
     4321 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     4381 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata
     4441 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     4501 cgagggtcag ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     4561 gtagctcttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa
     4621 cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa
     4681 tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa
     4741 cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg
     4801 cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat
     4861 tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc
     4921 ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag
     4981 ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg
     5041 acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa
     5101 caatagatat agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa
     5161 ttccagcaga tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag
     5221 gcgaagcaga tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg
     5281 tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc
     5341 tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta
     5401 atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc
     5461 aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat
     5521 tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg
     5581 cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg
     5641 caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg
     5701 ttcaaaaagt gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag
     5761 catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta
     5821 aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg
     5881 tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa
     5941 tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag
     6001 gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag
     6061 aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag
     6121 ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc
     6181 cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg
     6241 atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg
     6301 gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag
     6361 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     6421 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     6481 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     6541 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     6601 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     6661 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     6721 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     6781 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     6841 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     6901 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     6961 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     7021 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     7081 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat
     7141 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     7201 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     7261 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     7321 ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct
     7381 aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     7441 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     7501 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     7561 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     7621 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     7681 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     7741 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     7801 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     7861 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     7921 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     7981 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     8041 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     8101 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     8161 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     8221 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     8281 gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac
     8341 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     8401 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     8461 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     8521 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     8581 aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta
     8641 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     8701 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     8761 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     8821 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     8881 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     8941 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     9001 catttcatcg ttttggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     9061 ttggatgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     9121 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     9181 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     9241 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     9301 tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca atccgtctga
     9361 atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt
     9421 cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca
     9481 aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg
     9541 ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc
     9601 cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca
     9661 agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct
     9721 ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac
     9781 aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa
     9841 tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag
     9901 aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag
     9961 tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg
    10021 atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt
    10081 acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca
    10141 tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg
    10201 gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg
    10261 tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc
    10321 aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac
    10381 tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa
    10441 gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat
    10501 gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg
    10561 actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa
    10621 attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa
    10681 ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg
    10741 acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc
    10801 aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg
    10861 aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac
    10921 ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg
    10981 ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga
    11041 aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg
    11101 accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag
    11161 tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact
    11221 atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga
    11281 aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg
    11341 gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg
    11401 tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga
    11461 caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc
    11521 acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa
    11581 aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa
    11641 attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac
    11701 tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca
    11761 aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag
    11821 aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg
    11881 gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga
    11941 tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag
    12001 gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga
    12061 aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac
    12121 tgccggagct tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag
    12181 aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg
    12241 atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg
    12301 atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg
    12361 aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg
    12421 aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa
    12481 acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt
    12541 cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa
    12601 caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg
    12661 gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca
    12721 gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa
    12781 aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc
    12841 ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa
    12901 caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga
    12961 ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat
    13021 tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga
    13081 cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac
    13141 atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc
    13201 ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg
    13261 gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac
    13321 tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct
    13381 gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt
    13441 ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc
    13501 ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg
    13561 ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa
    13621 acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca
    13681 gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga
    13741 aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc
    13801 agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct
    13861 tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt
    13921 tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa
    13981 ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt acttattttt
    14041 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
    14101 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
    14161 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
    14221 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
    14281 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
    14341 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
    14401 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
    14461 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
    14521 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
    14581 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
    14641 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
    14701 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
    14761 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
    14821 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
    14881 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
    14941 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
    15001 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
    15061 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
    15121 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
    15181 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
    15241 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
    15301 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
    15361 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
    15421 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
    15481 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
    15541 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
    15601 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
    15661 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
    15721 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
    15781 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
    15841 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
    15901 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
    15961 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
    16021 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
    16081 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
    16141 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
    16201 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
    16261 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
    16321 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
    16381 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
    16441 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
    16501 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
    16561 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
    16621 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
    16681 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
    16741 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
    16801 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
    16861 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
    16921 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
    16981 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
    17041 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
    17101 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
    17161 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
    17221 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
    17281 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
    17341 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
    17401 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
    17461 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
    17521 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat cagacagaga
    17581 aagaaccctt ggtattcaag ggttcttttt ttattatctg tttttttata ttaacgattt
    17641 ctatttggta catttttgat taataaaaag gggcaaaaaa ggggcaacaa gttacagttt
    17701 ttctatatct tctttcaatt tttcttttac tccttttgtc acatgcaaat aaattttttc
    17761 agtgatatca ctattttcgt gaccaacgcg atcctgaatg gcatacagag gagttcctag
    17821 ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt ttttatcaat
    17881 cttcatgtca gctttatgac ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt
    17941 tcctttcgtt gtttgaaata gaaattgacc atttggattt aattctagaa gctcattgta
    18001 aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccggcagctg tttttgtaga
    18061 atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc catttataac
    18121 tactgatgca ttattattgg taatgtgtac atcatcttta cttaatgcga ttgcttcacc
    18181 agacctcatc ccagtcaaat atagccactg gaaaaggaga gaatatcttt tattgtgtga
    18241 agttgtgaaa tctactagtc tattatattc atcatcttct aaaaatttat ctttaatttt
    18301 tatcgttttt gactctcttt tataatcaat aactacttca tcgataggat ttttttctac
    18361 ataacctttt ttcatagcat atgaaaaaag aagattcaat ttggatttaa ttacactgac
    18421 gtacttattt gacaagtcat ctttatatat catgtcttca aaaatattat tcaaatctat
    18481 tgtattaata ccagaaacga tgtaagtctc aggtatcttc ttctttatag tgtttaatat
    18541 attattcgta ggataatatg tagattcctt aacttgtctt ttgtatataa ctaaccattc
    18601 ttcaacaagc tcatgaaatg ttagatcagg cttatgaagt gttttttcgt ttaattttat
    18661 atcaattttt ttatttaact ctagcaatgc cactttctga gtttctcgag atttattttt
    18721 ataagtaatg ctcacttttt ttctttttct agtttttgga tcaatatatc gctcattata
    18781 tttatatact ttttttccgt ttttatcagt tttagtttcg atccacattg ttttcatctc
    18841 ctatttgttg ctataatagg aatagataag taagccgatt atagcaggtt tatttttcat
    18901 cacgtccaca aactttggcg agggaggggc gtgtttttgt tagttattgt ggtaggttat
    18961 ctaaagcgta ttgtgcttgc tcatttgtaa aaccttcaaa tataagttga tcgtacaatt
    19021 gcgaatctga cattgatgcc caatcatcgt aactttccgc tttagccaaa gcttgttcgt
    19081 tgtaatcgac atcaacatta gccagcgcat aatcgattgc atcacttgga tattcttcaa
    19141 actctagttg ttcacgcaaa ccttgttcag acataccagc ccaacctaga tagctttcag
    19201 ctttgctcaa tgcatttctg tattcacgag gaacactttc ttcttgagta acttcttcta
    19261 tagtagtttc agtagtttca gtttcttctt caacgactgt agattctgct accgattctg
    19321 cagaagacac ttctgtcacg ctttctttag ttgtttcagc gacggtattt ttagcatcat
    19381 cactgcctcc ttgcgaacct atagcaataa acaccacaac aaccgctaac aaccaaaacc
    19441 atacccgttt ataaaacggt ttcttaactt tatacatttt cccatcttga cccataactt
    19501 tttttgccat ttaaatatcc ctcgcttctt gttataatat atttgtgatc tcagaaatga
    19561 ggtatgagtc cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg
    19621 caaaatagta gggcataaaa atgtattatt gaattccgta tttagaaaat cctaattgaa
    19681 cttcaccgga agtcttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct
    19741 gtactttcca tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag
    19801 tgtcagcgtc ttgctgagcg cttgtttgtt ggtcttctgg attctgttga gatgctgctt
    19861 gttgttcttt ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt
    19921 cataaagggc cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg
    19981 gagttgactg taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt
    20041 ggattgtact ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttgg
    20101 agcttgtaac tgttgaacta gaatctgttt tagatgtaga tttgctagta gatgaactgg
    20161 tttcagttgt ttcttttgtt ttacttattt ttgtttcttg gttagaagtg gtatctgttg
    20221 attcagcttt tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca
    20281 taacgctaac ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac
    20341 gggctttttt atataagaaa acgataagcg ctttctggaa gcccgtaaag attctttaat
    20401 tcctcgattt ttttaggata ttgatcatta tcttctttat aaagagaaac aatgagatta
    20461 gcagcaaagc aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacataa
    20521 tagctggata agccacgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat
    20581 ctttcctcag agtctcgcag ttcatcattt aaaaagatta tcgcacgatc tctaatttct
    20641 tgaaactgcc ccttgggatt ctcgataaaa ggaacgtatt gaattttaat gcccatcttt
    20701 tcacaaatat aaaaaggatt agcggactgg tatttccgct tcaactcctc gactaaatta
    20761 atcgtatcca tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat
    20821 atcccagaaa gtcgctatca ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt
    20881 ttccccacca taagacatat taacatttga gtctagtagt ttatcaagtt caaccacttc
    20941 ctcttttgta gcccacttgg gaacattatt atttcccaat aaataatcag ttgtgacacc
    21001 aaaataatca gcaactttct ttaagttctc agattttggc gaggctttat cccatcttct
    21061 tatttgtcca ttagagatgc ccacctgtct ttctacttct gctatagtca catgcttttc
    21121 gtctgctaat tctttaatct tagtaactaa actcattatt atcaaccttt caaagctgaa
    21181 aagaaaataa atagcttaaa agttatattt ttggttgaca attagctttt aagctagtat
    21241 atttaattcg taagctaaat tgttagctaa ataagagcaa caaaaaactc tactaattta
    21301 aaacattctc tcggtcgcca aacttagaaa tgttatttta gaggcttttt ataagtctta
    21361 tttaactatg tattcataat agcttaaaag ctaataggtg tcaacgattt agctaatttt
    21421 ttagcttaca aattatttgt ttagaaagga gctattttta tgtctgagaa tttagactta
    21481 aaaattcgag cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc
    21541 ggtatttcag gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca
    21601 caacagcata tcgaaacagt gaaaaaaata ttggacatcc gatagggggg gtaggcgatg
    21661 acaaaactaa aaaaacaaga ttttgtaaaa aaatataatt attctccatc tacttatcaa
    21721 cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca
    21781 ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat
    21841 aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat
    21901 ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcatta
    21961 gtattcagcg caccgctaat cgtctatgct ttagggcttt ggggaagtag acaagcgttg
    22021 ataggaacga ttgtgtacat ggtttggatt tttatagggc tggatgaagc tgagtacaaa
    22081 acaaaaaagc cagtcgggag ggactgacta atgagtaagg acgatattgt atttctgtta
    22141 attatgtttt gtgggattgg atcaataata attaatttgt atttgattat taaagaaaaa
    22201 tttttcaatt aaaatgagtc ttttagcttt aacttaaatt ttttttgata attagctgtt
    22261 cttatgacca ttacaagttt ttcaccaggt aatatatctt tcatatctaa aacttctgaa
    22321 aaagcgactg agtagccagg agagattgct tgtgccaacg gtataggaga taaaaggcta
    22381 cttttatgaa tgcttcccat atcaaaacca ttggcgtgat aatgtgaaca attaattttc
    22441 ttaccgttgt aaagatacgt acattcgata attgagtttg gcatttttga tgggtttgca
    22501 attacgccag taagaatcat tcgattactt ctatcatgct taactaaaaa ataggagagc
    22561 gtaatttttc ttttatcttt aaataagtta tagagggata aaaaaaatcc tgaaatagaa
    22621 atgattaaag taatagcgac ttggtaatcg ctcaaaattt ttatcaagta attcaccacc
    22681 tttttactca aattatatca aaaaggagag aagaaataat gcaagaatta gtaattttga
    22741 aaaataaaaa aagacacgaa cgccggcaag catgatcgtg tcagggaaat aaactatctt
    22801 tctatatttt accacagaaa ggaatgaacc gtaaatgcta attgcaacgg atacactaga
    22861 ccgcatcttt ttaaaagacg aatacaaact gcgcaatata gatgcgtcag gaattttagt
    22921 atttgatctt tatgacaatg gaaaaattgg tatctatcaa gcaagtgata ttgaagaaac
    22981 aaaccttgct ttcgaacaaa ttgatgattc tgtggaattg gatttagatg aggcaatcct
    23041 agcttttgaa caaattgcaa aattattaaa ggaggcacaa aagaatggca actctttacc
    23101 aactcagcga gtcatatatc aaagtcctgg aactggcaga acaattggat gaagaaattc
    23161 ttcgcgatac tctcgattcg attaatgaag cgatcgagta taaggcagaa aacttagcaa
    23221 aaatagttaa agaagtagaa gggaaagctg agttaatcga tagtgaaatt aaacgtctgc
    23281 aggaacgtaa gacatcgctt ttgaacaatg ctaagagtat caagcactat ttacaagagg
    23341 aaatggaaaa gactggtaaa acgaagatta aaggtgaatt attcaacatt ggaattcaaa
    23401 ataatccagt atcggtcaat gtaatcaatg agaacttaat tccaaaagga ttctttaccc
    23461 ctgtgcctcc caaattggat aaaaagcaat tgaaggagga actgaagcac ggagatattc
    23521 ctggtgctga actcgtccaa acaaaaggtt tgaggataag atgatgaagg aggagaaata
    23581 atggaacacg aaaacgtaaa agacgctttg aaagctgcta tcgaaatcgc ggaagctaaa
    23641 ggaataaagg ttgacggcaa accagcaact gtccatgaca ttcaaaattt gacgaaagaa
    23701 catctttatt ttatcgccga cttgcttggt ttgtcggaat tgtatttaga caaataaccc
    23761 cacacggaag gagtggctag ggtggttgta ttacaagtaa tagtctgcgt aacattcatt
    23821 tggtcgtgca taccggtttg tatatatatc atgaagaagc ttcgccctga gtcttacaaa
    23881 ttatacgcaa tttacagcat aacagtagct cttgcattta cattgtttgc aatttctagg
    23941 cttccgtttt aatcgtggga atgaattttt aatgaactaa attatctata aagcaaaaaa
    24001 aggagaaaca acattgaaaa acaaactagc aaaaacaaca gcaatcatcg gactagcact
    24061 aggtagcggc gttatcggct acgcagcaag caatgcgttt caagatttgg acacgatcaa
    24121 agcaaatttc aacacagtcc tacaatacgg tcaaacgaaa tcgcaacgtg tgtcagaact
    24181 tgaatcacag ctatccaaca acactcgcac acaggagcag ctgaaagccg aaattgagca
    24241 aatcaaatcg gacaagcaga aggaaattga agccaagcaa cgagaaatcg aacaaaagca
    24301 acaggagatc gctacaaagc aacaggaagc cgatagcttg cgccaacaac tatccacagt
    24361 gcaaaacgac aaggagcagt tagaacagcg cgtgagcgag ttacggcagt atacggatca
    24421 aaaggtaggg gagttgggga aatgaaagag ttgactattt atgacccaaa tatttcatgg
    24481 gctaaacaca caattaaagt ttcttttatg atttggggtt acaaaggata tgttacttac
    24541 aaagtgggcg gtaacactaa gggtttatca ttaatagcta ttgattcaga tgatctatat
    24601 gatgctaatt tcgaggataa cccagttaat ttccgtgacc ttgatgaaga ttggttctct
    24661 atggaactta ctaacgataa aggcgacagt acacttgtag aagatgaatt cgacagacta
    24721 ggtgattata ttgtcggtgt tgaaattatt gcgcacgaac cagagtagga gagtgaatac
    24781 aaaatgatta aaagaatcac tatatcagtt gaagaagata gtggatcaac aatgcagcat
    24841 accgacaatc gaaaaacttt tttaaccaat agaaaggaga attttgatgc agattaagaa
    24901 ggcgacggat atcgaacaaa ccaagggaac atatttgatc tacggtgctc caggaaaagg
    24961 taaaacttca acggctaaat attttccagg gaaaacactg atccttgata ttgatcggac
    25021 ttcaaaagtc cttagaggtg agaaaaatgt cgatatcgta tatatcgaca acgaagatac
    25081 ctggaacgac tggggcaaca cgttggctga tttaactacc aattatgtag gtgtttatga
    25141 caacgtggtt gtcgataatg tcagcgaatt ggaacggtgt attctgtcca gtttaggcgc
    25201 agaaggaaaa aacaacggcg ttccttcgca aggcgattat caatatatgc aatttagaat
    25261 ggtgaacagc ctacgctata tgaaaaattt agacagcaac cttatctgga ccgcatggga
    25321 agaaatcgat ttgtggacgg attcggatgg atcttcttac aacatcgctt tgccgcagat
    25381 caatcgaaag attcgaaaca atattttggg attatgtgac gttgttggtc gattgatggt
    25441 aaaggaagac gatgaacgcg gatttatttt acaagccaca gattcaacct atgccaaaaa
    25501 tcaattggat aatagatctg gctgtaagca aagtgagctg attttatatg gaattgtacg
    25561 actaccagaa tgatttagtc gatcgtgcaa gacaagccta tgttgatggt tacaaggcac
    25621 catgtatcgt atcaccgtgc ggttctggaa aatcagtcat ggttgcagag atcgctaaac
    25681 gaaccacacg aaaaggcaac agagtgttgt tcttggtcca tcgtcgtgaa ttgattgatc
    25741 agatcaaaga tacgtttcaa aagatgggcg tagataatca gcttgtaaat ttcggcatgg
    25801 ttcaaacagt cgtacgacat cttaaaacca tcaagaaacc acaactgatc atcactgacg
    25861 aaaaccatca cgggttagca gcgtcctaca gaaaaatcta tgagtattat tcggacgttc
    25921 cgcgattagg ttttaccgcg acacccatcc ggttaaacgg tagtgggttg ggggatgtga
    25981 acgacttgct aattgaagga gtatcggcta aatggttgat tgagaatcat agattagctc
    26041 cttatgaata ttatgcacct aaattgatcg atacagccga attaaagaaa gcttcgactg
    26101 gagattttac aaagaaatca atggataagg cagtgaagaa tactatttat ggcgacgtgt
    26161 tgaaacatta taaaacgtta gcagaaggtg aacaggcaat agcatactgt cacagtatcg
    26221 atgcaagtaa acacactgct gaaatattca acgatgctgg ttataaagca acacatatcg
    26281 atgcaaaaac tccaaaagat gaacgtgcag atattatcga agcgttcaga aatcatgaaa
    26341 ttaaaatcct atgcaatgtt gatttgatcg gagaagggtt tgatgttcct gattgttcga
    26401 ctgtaattat gttgagacct actcaatcgt tatcattgta cattcagcaa tcgatgaggg
    26461 ggatgcgtta ccgaccagat aaaacgtcaa tcatcattga ccatgtgggt aatgtgaacc
    26521 gttttggatt gcctgatatg gatcgtgaat ggagtttaga tacaaaaaag aaaacaaaaa
    26581 ctgatagtga tttatctatc gttcaatgcg cgttttgttt tggtgcttac gaacggccaa
    26641 aaggcgataa tatttgtccg tattgcggag agttgcagcc gattgaagaa cgaaagtcag
    26701 agttagaaat cgatgaatcg gctgaattaa tgaaagtcgg agaaacgaaa attactttga
    26761 attttgagaa caataaatat tacaacatga cggaagatga agcagaaagt atagaggact
    26821 tatatgccat agcaagagct aaaggattta aacctggttg ggcttatatg gctgctaaga
    26881 ggaaggggtg gttgtaggaa tgggcggtat ttttggtaaa aaagcagaag atgttcaagg
    26941 ttcaataata aatggcgtaa caataatagg tgatactgga aaaagagata gttcaggcaa
    27001 tcaaattgta atcgctagaa ataaaaataa cgaagttttt gaatcttctc tatctaatat
    27061 aaaaagaggt gttgttaacg ggacaggcga agagagtggc tacgttaatt taaaaaaagc
    27121 taatgaaaaa taccatgttg aaggaactta cgttaaggct ttgtctgtcg gtggaacaaa
    27181 aggcgttaat tgggataaac gaagaaataa atggaaagca cgattatatt tcaaaggaga
    27241 atatgtctta caaaaggatt tcaacaccaa agaagaagca atccaagcac gacttgcagc
    27301 agaagaaaaa tatttcaaac ctatcttaga aaaatatgaa aataaaaagg agaatgatta
    27361 acatgacagg atttaactta gacttttcaa aagcgcaaca agggaacgaa atcaaagacg
    27421 gtacgtacga agtggtcgta aacaaagctg tagaaaatgc aactaaatca ggcgcagaat
    27481 ttatcgatat cgatttgatc gttcgtaacg atgtggatca accgttccaa aataaacaca
    27541 ttttcgcaaa aatctggaaa gccaaagcta ccggcaaata taacgaaggt atgatcatgg
    27601 caattgcaca agcgctacag ttggaagacg gaaaatcata taacgggttt gatgaattgc
    27661 tggcggactt tgttttgaaa accgcctcag tacgcgtgaa aactgaagag tcaaatggat
    27721 ataaaaacgt caatgtgaag tcttgggata aaacgaacac acggggcgta atgaatcacc
    27781 aattcaaaaa tggtgatgaa ccatccttcg gaccggaacg ttcaagagcg acaacggtta
    27841 gaaatgacaa tcttccattt taattgaggt gtaatcaatg tatgaaaaaa ttccaaaaga
    27901 gcttaaaaat ctcaaacaat ggtgtgttta taagctagtt tgggatgaaa agcggcagaa
    27961 gcacacgaag atcccttata acgccaacaa cgggcataaa gccaaatcca atgacgagag
    28021 tacctggtct gattttcaga ccgctctcgc tgctattgat cactacggaa tgagtggact
    28081 ggggttcttt ttcaaaccac cttatttcgg cattgatatc gataatgctg aaggtgaagt
    28141 tgagcgattt aaatcaggtg atatagaaga aaatattatc tatgaattta tcgaaagcat
    28201 gagatcctat gctgagtatt ctcaatcagg tactggcatc cacattattg ctcgcggaga
    28261 attgcccggc ggaagacgca ggaaaggtga cgtagaaatg taccagaacg gccgattctt
    28321 cgttatgact ggaaatgccg catcaaaata tttagaaata actgagccga atccaaagga
    28381 tatcaaacgc ctatatgatc gctacgttgg agatgaaaaa atcatccaat tcaaagaaga
    28441 aaatccgctg atgaataccg ttgatttacc ggttgaagaa attatcaaac gtgctgaatc
    28501 gtcttcccaa ggggcaagat tcaaagtctt tatgaacggc ggttgggaag ctttttattc
    28561 ctcgcaatcc gaagcggata tggcgttcgc caacgacttg gctttttgga ccggtagaga
    28621 ctttgaaaaa atggatgaga ttttcagagc gtctgcaatg atccgtagca aatatgacca
    28681 aaaaagggga tcggcaactt acggagaatc tctactaaat aaggcaattg ctgatacaaa
    28741 ttccgtctac aaccccaaac ggaaatctga ttttaagatt tttatcaaag accaggaaca
    28801 gccaaaagag gaaaaatact attcgtacga cgacaccggc aacgcggatc ggtttactga
    28861 tatctacgga acgttagtca aatattcgta cattgataaa tcttggtatt actacgatgg
    28921 gaaagtttgg ctacaggaca ataccggcga agttcggaaa atgatcgata cgacagtaga
    28981 catcatggga aaagaaccgt taacgatccc tgaaggcgca gacgatgaaa ccaaagaagc
    29041 tttgatgaaa gcgaaagaaa aacacgtcaa gcgttctcgc agcaatgccg gaaaaaacgc
    29101 tatgatggac gaattgaaac atcgactatc cgtattgccg gaagagttcg acaaagataa
    29161 aacgctgttc aacacacaaa gcggctatct gtctttgcat gacggtcttc tacacgagca
    29221 tgagatagac aaaatgttta cacgtgtttc aaatgtcgag tacacagaat cggtcgattg
    29281 tcctatgtgg gaagaattta ttaatcagat ttttgacaat gatcaagaat taatcagata
    29341 tatccagaaa tgtgtcggat attccctgac cggatctacc ggagagcaat gtatgttcat
    29401 cctctacggt catgggtcaa atggaaagtc ggtatttttg gaaattattt ctgaattgat
    29461 gggaaactat gcgatgacta tgcaggcaca aacgatcatg gtcaagcaaa gccaatcatc
    29521 agcaaacagt gatattgcac gattgaaagg tgcgcggttg gtcacttcgt cagaaccgaa
    29581 tgaaggcgtc cgactggacg aaggtctggt caaacaattg actggtggtg ataaagtcac
    29641 ggctcggcac ctttatggaa aagaattcga gttcgaacct gaattcaagt tgtggctggc
    29701 aacgaaccac aagcccatca tccgtgggac cgatgacggc atctggcgac ggttaaattt
    29761 aattccattc actgtccaaa tcccggatca caaaaaggat aaaaatctga aatttaaatt
    29821 gcagacggag ttgcaaggga tccttaaatg ggcaatcgat ggttgcctgt tgtggcagag
    29881 agagggcttg gagaaaccaa agtcggttgt tgcagcgagt caggattatc gaaacgaaat
    29941 ggatcaaata ggtacattta tcgaaacgtg ttgcgaaaca ggtccaggac taaaaatatc
    30001 aggcggagaa ttatacaaag tttatcgtga atgggcatca gataatggcg agcatacttt
    30061 cactaacacg aagtttggca gagagatttc aaagaaatat agcaaagaaa aagctggtgg
    30121 atttatggtt tataaaggaa tcacattaaa accaagaaaa tacgataacg ttagagaact
    30181 ttttaaataa atctggaggg ttctggaggg ttgagaacga aaccctccaa cccttagaaa
    30241 cgttgatata tcagtattta tattatattt ttctttcttt ctggagggtt ttaaaaaaat
    30301 aataaagtat taaagtaaaa aataaaaagt aaattatata aaaaagaata ggtttttggg
    30361 tctgaccctc caaactctcc accaaaatct tcctaaatgc tttgatacca acgattaaac
    30421 agtctggagg gttacattga aatcagaaca tcaaatacaa gacgagatca gaattgcctt
    30481 atcaaaacat ggttgcacga tttttcgatc caatgcggga caagtgacaa cgatcgaagg
    30541 gcggcaattt tacgggatgc caaaagggtt cccagatcta tgtggacatc gtaattctga
    30601 tgggaaagca atttatatag aagtaaaaaa tgaacgcggt aagttgcgtg aagatcaaaa
    30661 aaggtttgct gaatttttaa acggtcaacc tgtactatac ggtgttgcac gatcagtgga
    30721 agaagcattg aaaattgtgg aggaatcatc atgacaacag aagaagtgat tcaaatgcgt
    30781 attcgaagcc ttcagcgtga gattgacgat ctggaacgga caaaggcagt gatggtcaat
    30841 gaaacggcga ggaaggcaat cgatgtgcac atagagaatt taagaaggga aatccgtaga
    30901 ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg ctgattcaag
    30961 atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta g
//
LOCUS       DASHWH010000029        29111 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.029, whole genome shotgun sequence.
ACCESSION   DASHWH010000029 DASHWH010000000
VERSION     DASHWH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.029"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1031)
                     /locus_tag="U2R41_001388"
     CDS             complement(<1..1031)
                     /locus_tag="U2R41_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8888809.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="U2R41_001389"
     CDS             complement(1113..1802)
                     /locus_tag="U2R41_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8888810.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U2R41_001390"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U2R41_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEM8888811.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="U2R41_001391"
     CDS             complement(2329..2832)
                     /locus_tag="U2R41_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEM8888812.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U2R41_001392"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U2R41_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEM8888813.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U2R41_001393"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U2R41_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEM8888814.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="U2R41_001394"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="U2R41_001394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEM8888815.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="U2R41_001395"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="U2R41_001395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEM8888816.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="U2R41_001396"
     CDS             complement(6444..7463)
                     /locus_tag="U2R41_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM8888817.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="U2R41_001397"
     CDS             complement(7636..8541)
                     /locus_tag="U2R41_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8888818.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U2R41_001398"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U2R41_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEM8888819.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U2R41_001399"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U2R41_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEM8888820.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U2R41_001400"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U2R41_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8888821.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="U2R41_001401"
     CDS             9754..10398
                     /locus_tag="U2R41_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8888822.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="U2R41_001402"
     CDS             complement(10454..10957)
                     /locus_tag="U2R41_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM8888823.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="U2R41_001403"
     CDS             11554..12519
                     /locus_tag="U2R41_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM8888824.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="U2R41_001404"
     CDS             complement(12618..13790)
                     /locus_tag="U2R41_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888825.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="U2R41_001405"
     CDS             complement(13777..14922)
                     /locus_tag="U2R41_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888826.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="U2R41_001406"
     CDS             complement(14936..15868)
                     /locus_tag="U2R41_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888827.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="U2R41_001407"
     CDS             15985..17064
                     /locus_tag="U2R41_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8888828.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="U2R41_001408"
     CDS             17061..17690
                     /locus_tag="U2R41_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888829.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="U2R41_001409"
     CDS             complement(17800..18027)
                     /locus_tag="U2R41_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8888830.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="U2R41_001410"
     CDS             complement(18130..20718)
                     /locus_tag="U2R41_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEM8888831.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKTVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="U2R41_001411"
     CDS             complement(20738..21397)
                     /locus_tag="U2R41_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8888832.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="U2R41_001412"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="U2R41_001412"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEM8888833.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="U2R41_001413"
     CDS             complement(22170..23036)
                     /locus_tag="U2R41_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8888834.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="U2R41_001414"
     CDS             23145..23786
                     /locus_tag="U2R41_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEM8888835.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="U2R41_001415"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="U2R41_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEM8888836.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="U2R41_001416"
     CDS             complement(25227..26126)
                     /locus_tag="U2R41_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8888837.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="U2R41_001417"
     CDS             complement(26128..26925)
                     /locus_tag="U2R41_001417"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEM8888838.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="U2R41_001418"
     CDS             complement(26903..27607)
                     /locus_tag="U2R41_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEM8888839.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="U2R41_001419"
     CDS             27738..28313
                     /locus_tag="U2R41_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEM8888840.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="U2R41_001420"
     CDS             28431..29108
                     /locus_tag="U2R41_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM8888841.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5233 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agcttatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacagttt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa a
//
LOCUS       DASHWH010000030        27909 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.030, whole genome shotgun sequence.
ACCESSION   DASHWH010000030 DASHWH010000000
VERSION     DASHWH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.030"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="U2R41_001421"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="U2R41_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEM8888842.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="U2R41_001422"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="U2R41_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEM8888843.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="U2R41_001423"
     CDS             complement(2738..3049)
                     /locus_tag="U2R41_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEM8888844.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="U2R41_001424"
     CDS             complement(3046..3339)
                     /locus_tag="U2R41_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEM8888845.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="U2R41_001425"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="U2R41_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEM8888846.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="U2R41_001426"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="U2R41_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEM8888847.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="U2R41_001427"
     CDS             complement(5177..9529)
                     /locus_tag="U2R41_001427"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEM8888848.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="U2R41_001428"
     CDS             complement(9741..11450)
                     /locus_tag="U2R41_001428"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEM8888849.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="U2R41_001429"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="U2R41_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEM8888850.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDQDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="U2R41_001430"
     CDS             complement(12947..13747)
                     /locus_tag="U2R41_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEM8888851.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="U2R41_001431"
     CDS             complement(13744..14556)
                     /locus_tag="U2R41_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEM8888852.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="U2R41_001432"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="U2R41_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEM8888853.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="U2R41_001433"
     CDS             complement(15311..16033)
                     /locus_tag="U2R41_001433"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEM8888854.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="U2R41_001434"
     CDS             complement(16169..17050)
                     /locus_tag="U2R41_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEM8888855.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="U2R41_001435"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="U2R41_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEM8888856.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="U2R41_001436"
     CDS             18290..18769
                     /locus_tag="U2R41_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEM8888857.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="U2R41_001437"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="U2R41_001437"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEM8888858.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="U2R41_001438"
     CDS             20070..20855
                     /locus_tag="U2R41_001438"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEM8888859.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="U2R41_001439"
     CDS             complement(21201..22892)
                     /locus_tag="U2R41_001439"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEM8888860.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="U2R41_001440"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="U2R41_001440"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEM8888861.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="U2R41_001441"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="U2R41_001441"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEM8888862.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="U2R41_001442"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="U2R41_001442"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEM8888863.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27176..27877
                     /locus_tag="U2R41_001443"
     CDS             27176..27877
                     /locus_tag="U2R41_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8888864.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         7534 a         5971 c         4931 g         9473 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 ttgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccattt
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    26881 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa aattttttga ataaattaaa
    26941 aaaataaatt atattagcgt tgttagcgtt tactatatgt attattgttg ttttttatac
    27001 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
    27061 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
    27121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
    27181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
    27241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
    27301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
    27361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
    27421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
    27481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
    27541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
    27601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
    27661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
    27721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
    27781 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
    27841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
    27901 cctgatatt
//
LOCUS       DASHWH010000031        27580 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.031, whole genome shotgun sequence.
ACCESSION   DASHWH010000031 DASHWH010000000
VERSION     DASHWH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.031"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..333)
                     /locus_tag="U2R41_001444"
     CDS             complement(<1..333)
                     /locus_tag="U2R41_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888865.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(508..2643)
                     /locus_tag="U2R41_001445"
     CDS             complement(508..2643)
                     /locus_tag="U2R41_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8888866.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2726..4381)
                     /locus_tag="U2R41_001446"
     CDS             complement(2726..4381)
                     /locus_tag="U2R41_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8888867.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4383..4697)
                     /locus_tag="U2R41_001447"
     CDS             complement(4383..4697)
                     /locus_tag="U2R41_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM8888868.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4867..5127
                     /locus_tag="U2R41_001448"
     CDS             4867..5127
                     /locus_tag="U2R41_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEM8888869.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5141..5455
                     /locus_tag="U2R41_001449"
     CDS             5141..5455
                     /locus_tag="U2R41_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEM8888870.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5940..6494)
                     /locus_tag="U2R41_001450"
     CDS             complement(5940..6494)
                     /locus_tag="U2R41_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEM8888871.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6620..8548)
                     /locus_tag="U2R41_001451"
     CDS             complement(6620..8548)
                     /locus_tag="U2R41_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM8888872.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8854..9570)
                     /locus_tag="U2R41_001452"
     CDS             complement(8854..9570)
                     /locus_tag="U2R41_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM8888873.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="U2R41_001453"
     CDS             complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="U2R41_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEM8888874.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="U2R41_001454"
     CDS             complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="U2R41_001454"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEM8888875.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13040..14500)
                     /locus_tag="U2R41_001455"
     CDS             complement(13040..14500)
                     /locus_tag="U2R41_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888876.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14490..15785)
                     /locus_tag="U2R41_001456"
     CDS             complement(14490..15785)
                     /locus_tag="U2R41_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8888877.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16117..16794)
                     /locus_tag="U2R41_001457"
     CDS             complement(16117..16794)
                     /locus_tag="U2R41_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEM8888878.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16824..17915)
                     /locus_tag="U2R41_001458"
     CDS             complement(16824..17915)
                     /locus_tag="U2R41_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEM8888879.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18016..18450)
                     /locus_tag="U2R41_001459"
     CDS             complement(18016..18450)
                     /locus_tag="U2R41_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8888880.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19034..20491)
                     /locus_tag="U2R41_001460"
     CDS             complement(19034..20491)
                     /locus_tag="U2R41_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8888881.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20488..22383)
                     /locus_tag="U2R41_001461"
     CDS             complement(20488..22383)
                     /locus_tag="U2R41_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8888882.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22441..24216)
                     /locus_tag="U2R41_001462"
     CDS             complement(22441..24216)
                     /locus_tag="U2R41_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8888883.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24266..25762)
                     /locus_tag="U2R41_001463"
     CDS             complement(24266..25762)
                     /locus_tag="U2R41_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8888884.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25956..27578)
                     /locus_tag="U2R41_001464"
     CDS             complement(25956..27578)
                     /locus_tag="U2R41_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM8888885.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         7813 a         5943 c         4591 g         9233 t
ORIGIN      
        1 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
       61 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
      121 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
      181 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
      241 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
      301 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
      361 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
      421 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
      481 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
      541 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
      601 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
      661 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
      721 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
      781 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
      841 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
      901 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
      961 atggtagtca tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
     1021 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
     1081 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
     1141 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
     1201 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
     1261 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
     1321 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
     1381 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
     1441 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
     1501 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
     1561 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
     1621 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
     1681 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
     1741 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
     1801 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
     1861 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
     1921 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
     1981 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
     2041 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
     2101 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
     2161 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
     2221 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
     2281 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
     2341 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
     2401 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
     2461 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
     2521 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
     2581 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
     2641 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
     2701 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
     2761 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
     2821 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
     2881 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
     2941 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
     3001 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
     3061 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
     3121 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
     3181 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
     3241 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
     3301 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
     3361 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
     3421 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
     3481 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
     3541 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
     3601 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
     3661 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
     3721 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
     3781 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
     3841 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
     3901 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
     3961 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
     4021 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
     4081 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
     4141 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
     4201 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
     4261 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
     4321 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
     4381 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
     4441 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
     4501 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
     4561 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
     4621 aacgctggat tttccaaaat cttttccttt aattctggga ctgttatcga ttttattgat
     4681 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
     4741 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
     4801 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
     4861 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
     4921 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
     4981 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
     5041 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
     5101 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
     5161 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
     5221 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
     5281 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
     5341 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
     5401 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
     5461 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
     5521 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
     5581 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
     5641 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
     5701 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
     5761 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
     5821 aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta ttatcgagaa
     5881 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
     5941 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
     6001 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
     6061 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
     6121 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
     6181 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
     6241 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
     6301 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
     6361 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
     6421 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
     6481 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
     6541 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
     6601 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
     6661 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
     6721 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
     6781 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
     6841 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
     6901 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     6961 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     7021 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     7081 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     7141 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     7201 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     7261 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     7321 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     7381 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     7441 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     7501 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     7561 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     7621 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     7681 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     7741 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     7801 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     7861 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     7921 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     7981 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     8041 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     8101 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     8161 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     8221 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     8281 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     8341 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     8401 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     8461 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     8521 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     8581 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     8641 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     8701 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     8761 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     8821 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     8881 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     8941 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     9001 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     9061 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     9121 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     9181 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     9241 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     9301 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     9361 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     9421 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     9481 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     9541 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     9601 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     9661 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     9721 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     9781 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     9841 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     9901 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     9961 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    10021 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    10081 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    10141 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    10201 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    10261 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    10321 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    10381 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    10441 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    10501 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    10561 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    10621 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    10681 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    10741 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    10801 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    10861 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    10921 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    10981 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    11041 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    11101 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    11161 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    11221 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    11281 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    11341 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    11401 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    11461 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    11521 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    11581 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    11641 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    11701 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    11761 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    11821 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    11881 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    11941 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    12001 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    12061 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    12121 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    12181 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    12241 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    12301 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    12361 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    12421 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    12481 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    12541 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    12601 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    12661 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    12721 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    12781 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    12841 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    12901 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    12961 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    13021 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    13081 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    13141 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    13201 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    13261 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    13321 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    13381 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    13441 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    13501 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    13561 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    13621 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    13681 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    13741 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    13801 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    13861 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    13921 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    13981 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    14041 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    14101 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    14161 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    14221 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    14281 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    14341 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    14401 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    14461 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    14521 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    14581 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
    14641 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    14701 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    14761 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    14821 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    14881 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    14941 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    15001 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    15061 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
    15121 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    15181 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    15241 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    15301 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    15361 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    15421 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    15481 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    15541 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    15601 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    15661 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    15721 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    15781 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    15841 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    15901 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    15961 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    16021 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    16081 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    16141 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    16201 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    16261 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    16321 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    16381 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    16441 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    16501 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    16561 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    16621 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    16681 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    16741 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    16801 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    16861 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    16921 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    16981 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    17041 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    17101 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    17161 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    17221 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    17281 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    17341 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    17401 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    17461 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    17521 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    17581 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    17641 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    17701 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    17761 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    17821 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    17881 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    17941 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    18001 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    18061 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    18121 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    18181 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    18241 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    18301 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    18361 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    18421 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    18481 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    18541 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    18601 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    18661 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    18721 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    18781 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    18841 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    18901 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    18961 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    19021 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    19081 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    19141 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    19201 acgacgtata accaaataaa tctactccat cttcgatagc ttccttcatc tgctcaatat
    19261 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    19321 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    19381 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    19441 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc aaattttctg
    19501 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    19561 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    19621 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    19681 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    19741 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    19801 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    19861 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    19921 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    19981 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    20041 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    20101 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    20161 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    20221 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    20281 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    20341 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    20401 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    20461 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    20521 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    20581 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    20641 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    20701 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    20761 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
    20821 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    20881 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    20941 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    21001 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    21061 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    21121 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    21181 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    21241 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    21301 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    21361 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    21421 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    21481 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
    21541 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    21601 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    21661 aaccgtactt gtatatcttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    21721 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    21781 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    21841 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    21901 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    21961 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    22021 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    22081 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    22141 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    22201 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    22261 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    22321 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    22381 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    22441 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    22501 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    22561 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    22621 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    22681 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    22741 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctccgaaga
    22801 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    22861 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    22921 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    22981 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    23041 tcgaaaagga ttcaacgctt cggcaagata caaaaccaaa taagcgcttt cagctaaatt
    23101 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    23161 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    23221 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    23281 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    23341 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    23401 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    23461 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    23521 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    23581 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    23641 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
    23701 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    23761 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    23821 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    23881 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    23941 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    24001 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    24061 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    24121 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    24181 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    24241 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    24301 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    24361 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    24421 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    24481 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    24541 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    24601 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    24661 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    24721 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    24781 aataactaaa tgcaatataa tctaccgtgt tagcttgcaa gtactgttcg tctccatcta
    24841 acattactgg tgcacagtct tcttccttcc atatcctgta aatataagct ggataatgcc
    24901 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    24961 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    25021 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    25081 acatatgatg acaggcttga taaatctctt tttttgttag tgtagcaatg ggttcaagtt
    25141 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    25201 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    25261 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    25321 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    25381 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    25441 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    25501 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    25561 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    25621 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    25681 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    25741 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    25801 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    25861 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    25921 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    25981 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    26041 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
    26101 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    26161 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    26221 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    26281 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    26341 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
    26401 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    26461 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    26521 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    26581 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    26641 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    26701 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    26761 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    26821 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    26881 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    26941 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccacca
    27001 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    27061 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    27121 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    27181 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    27241 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    27301 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    27361 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    27421 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    27481 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    27541 tgataaacaa ctgaatggtg ccaccatttt tgttccatga
//
LOCUS       DASHWH010000032        27249 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.032, whole genome shotgun sequence.
ACCESSION   DASHWH010000032 DASHWH010000000
VERSION     DASHWH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.032"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(159..443)
                     /locus_tag="U2R41_001465"
     CDS             complement(159..443)
                     /locus_tag="U2R41_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888886.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(461..1351)
                     /locus_tag="U2R41_001466"
     CDS             complement(461..1351)
                     /locus_tag="U2R41_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEM8888887.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1371..1589)
                     /locus_tag="U2R41_001467"
     CDS             complement(1371..1589)
                     /locus_tag="U2R41_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEM8888888.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1559..3259)
                     /locus_tag="U2R41_001468"
     CDS             complement(1559..3259)
                     /locus_tag="U2R41_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEM8888889.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3526..4299
                     /locus_tag="U2R41_001469"
     CDS             3526..4299
                     /locus_tag="U2R41_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888890.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="U2R41_001470"
     CDS             complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="U2R41_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEM8888891.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5015..5117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5123..6094)
                     /locus_tag="U2R41_001471"
     CDS             complement(5123..6094)
                     /locus_tag="U2R41_001471"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM8888892.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="U2R41_001472"
     CDS             complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="U2R41_001472"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEM8888893.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8064..8882)
                     /gene="purR"
                     /locus_tag="U2R41_001473"
     CDS             complement(8064..8882)
                     /gene="purR"
                     /locus_tag="U2R41_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEM8888894.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9049..9873)
                     /locus_tag="U2R41_001474"
     CDS             complement(9049..9873)
                     /locus_tag="U2R41_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8888895.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9846..10526)
                     /locus_tag="U2R41_001475"
     CDS             complement(9846..10526)
                     /locus_tag="U2R41_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8888896.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10549..11487)
                     /locus_tag="U2R41_001476"
     CDS             complement(10549..11487)
                     /locus_tag="U2R41_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEM8888897.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11732..12595)
                     /locus_tag="U2R41_001477"
     CDS             complement(11732..12595)
                     /locus_tag="U2R41_001477"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEM8888898.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="U2R41_001478"
     CDS             complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="U2R41_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM8888899.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15451..16257
                     /gene="proC"
                     /locus_tag="U2R41_001479"
     CDS             15451..16257
                     /gene="proC"
                     /locus_tag="U2R41_001479"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEM8888900.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16336..17892)
                     /gene="rny"
                     /locus_tag="U2R41_001480"
     CDS             complement(16336..17892)
                     /gene="rny"
                     /locus_tag="U2R41_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEM8888901.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18303..19352)
                     /gene="recA"
                     /locus_tag="U2R41_001481"
     CDS             complement(18303..19352)
                     /gene="recA"
                     /locus_tag="U2R41_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEM8888902.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19461..20702)
                     /locus_tag="U2R41_001482"
     CDS             complement(19461..20702)
                     /locus_tag="U2R41_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEM8888903.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="U2R41_001483"
     CDS             complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="U2R41_001483"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEM8888904.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21729..22652)
                     /locus_tag="U2R41_001484"
     CDS             complement(21729..22652)
                     /locus_tag="U2R41_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8888905.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23391..24677)
                     /locus_tag="U2R41_001485"
     CDS             complement(23391..24677)
                     /locus_tag="U2R41_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM8888906.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24670..25938)
                     /locus_tag="U2R41_001486"
     CDS             complement(24670..25938)
                     /locus_tag="U2R41_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM8888907.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26190..26651
                     /gene="mscL"
                     /locus_tag="U2R41_001487"
     CDS             26190..26651
                     /gene="mscL"
                     /locus_tag="U2R41_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEM8888908.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26710..27156)
                     /locus_tag="U2R41_001488"
     CDS             complement(26710..27156)
                     /locus_tag="U2R41_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8888909.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7742 a         5877 c         4727 g         8903 t
ORIGIN      
        1 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
       61 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
      121 tctgattatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
      181 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
      241 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
      301 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
      361 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
      421 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
      481 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
      541 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
      601 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
      661 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
      721 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
      781 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
      841 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
      901 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
      961 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcgcg aagcgttccg
     1021 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
     1081 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
     1141 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
     1201 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
     1261 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
     1321 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
     1381 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
     1441 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
     1501 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
     1561 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
     1621 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
     1681 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
     1741 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
     1801 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
     1861 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
     1921 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
     1981 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
     2041 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
     2101 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
     2161 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
     2221 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
     2281 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
     2341 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
     2401 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
     2461 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
     2521 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
     2581 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
     2641 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
     2701 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
     2761 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
     2821 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
     2881 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
     2941 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
     3001 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
     3061 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
     3121 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
     3181 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
     3241 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
     3301 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
     3361 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
     3421 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
     3481 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
     3541 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
     3601 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
     3661 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
     3721 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
     3781 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
     3841 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
     3901 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
     3961 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
     4021 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
     4081 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
     4141 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
     4201 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
     4261 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
     4321 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
     4381 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
     4441 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
     4501 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
     4561 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
     4621 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
     4681 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
     4741 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
     4801 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
     4861 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
     4921 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
     4981 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
     5041 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
     5101 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
     5161 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
     5221 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
     5281 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
     5341 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
     5401 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
     5461 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
     5521 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
     5581 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
     5641 tcaaatgatc gactggaata tcaaagaaac cttggatttg aactgcatga aggtccaaag
     5701 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
     5761 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
     5821 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
     5881 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
     5941 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
     6001 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
     6061 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
     6121 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
     6181 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
     6241 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
     6301 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
     6361 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
     6421 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
     6481 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
     6541 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
     6601 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
     6661 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
     6721 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
     6781 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
     6841 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
     6901 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
     6961 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
     7021 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
     7081 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
     7141 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
     7201 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
     7261 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
     7321 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
     7381 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
     7441 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
     7501 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
     7561 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
     7621 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
     7681 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
     7741 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
     7801 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
     7861 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
     7921 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
     7981 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
     8041 ctaagcagct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
     8101 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
     8161 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
     8221 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
     8281 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
     8341 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
     8401 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
     8461 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
     8521 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
     8581 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
     8641 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
     8701 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
     8761 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
     8821 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
     8881 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
     8941 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
     9001 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
     9061 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
     9121 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
     9181 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
     9241 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
     9301 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
     9361 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
     9421 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
     9481 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
     9541 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
     9601 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
     9661 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
     9721 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
     9781 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
     9841 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
     9901 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
     9961 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    10021 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    10081 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    10141 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    10201 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    10261 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    10321 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    10381 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    10441 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    10501 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    10561 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    10621 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    10681 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    10741 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    10801 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    10861 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    10921 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    10981 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    11041 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    11101 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    11161 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    11221 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    11281 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    11341 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    11401 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    11461 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    11521 ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc
    11581 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    11641 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    11701 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    11761 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    11821 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    11881 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    11941 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    12001 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    12061 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    12121 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    12181 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    12241 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    12301 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    12361 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    12421 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    12481 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    12541 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    12601 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    12661 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    12721 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    12781 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    12841 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    12901 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    12961 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    13021 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    13081 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    13141 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    13201 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    13261 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    13321 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    13381 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    13441 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    13501 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    13561 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    13621 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    13681 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    13741 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    13801 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    13861 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    13921 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    13981 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    14041 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    14101 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    14161 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    14221 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    14281 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    14341 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    14401 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    14461 ctactgctac atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa
    14521 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    14581 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    14641 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    14701 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    14761 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    14821 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    14881 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    14941 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    15001 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    15061 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    15121 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    15181 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    15241 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    15301 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
    15361 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    15421 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    15481 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    15541 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    15601 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    15661 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    15721 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
    15781 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    15841 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    15901 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    15961 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    16021 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    16081 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    16141 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    16201 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    16261 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    16321 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    16381 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    16441 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    16501 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    16561 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    16621 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    16681 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    16741 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    16801 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    16861 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    16921 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    16981 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    17041 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    17101 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    17161 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    17221 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    17281 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    17341 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    17401 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    17461 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    17521 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    17581 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    17641 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    17701 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    17761 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    17821 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    17881 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    17941 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    18001 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    18061 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    18121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    18181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    18241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    18301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    18361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    18421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    18481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    18541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    18601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    18661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    18721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    18781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    18841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    18901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    18961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    19021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    19081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    19141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    19201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    19261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    19321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    19381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    19441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    19501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    19561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    19621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    19681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    19741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    19801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    19861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    19921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    19981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    20041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    20101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    20161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    20221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    20281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    20341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    20401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    20461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    20521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    20581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    20641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    20701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    20761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    20821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    20881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    20941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    21001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    21061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    21121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    21181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    21241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    21301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    21361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    21421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    21481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    21541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    21601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    21661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    21721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    21781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    21841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    21901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    21961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    22021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    22081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    22141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    22201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    22261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    22321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    22381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    22441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    22501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    22561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    22621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    22681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    22741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    22801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    22861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
    22921 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
    22981 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
    23041 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
    23101 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
    23161 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
    23221 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
    23281 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
    23341 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
    23401 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
    23461 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
    23521 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
    23581 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
    23641 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
    23701 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
    23761 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
    23821 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
    23881 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
    23941 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
    24001 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
    24061 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
    24121 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
    24181 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
    24241 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
    24301 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
    24361 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
    24421 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
    24481 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
    24541 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
    24601 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
    24661 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
    24721 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
    24781 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
    24841 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
    24901 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
    24961 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
    25021 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
    25081 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
    25141 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
    25201 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
    25261 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
    25321 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
    25381 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
    25441 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
    25501 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
    25561 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
    25621 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
    25681 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
    25741 ctctgcaagt tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa
    25801 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
    25861 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
    25921 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
    25981 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
    26041 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
    26101 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
    26161 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
    26221 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
    26281 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
    26341 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
    26401 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
    26461 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
    26521 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
    26581 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
    26641 cttctaaata ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt
    26701 tttagattct taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc
    26761 caaatacaca tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc
    26821 ttgtgcgccg atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc
    26881 ttgaacaatc tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc
    26941 aaaatggata ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt
    27001 atcttgaaaa aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca
    27061 ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt
    27121 tgacaattct tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt
    27181 attcttagca taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg
    27241 tttgctttt
//
LOCUS       DASHWH010000033        26940 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.033, whole genome shotgun sequence.
ACCESSION   DASHWH010000033 DASHWH010000000
VERSION     DASHWH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.033"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            476..1339
                     /locus_tag="U2R41_001489"
     CDS             476..1339
                     /locus_tag="U2R41_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8888910.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U2R41_001490"
     CDS             complement(1468..3090)
                     /locus_tag="U2R41_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8888911.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U2R41_001491"
     CDS             3339..3860
                     /locus_tag="U2R41_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM8888912.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U2R41_001492"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U2R41_001492"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEM8888913.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U2R41_001493"
     CDS             5941..6759
                     /locus_tag="U2R41_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEM8888914.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U2R41_001494"
     CDS             complement(6862..7059)
                     /locus_tag="U2R41_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888915.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U2R41_001495"
     CDS             7228..7806
                     /locus_tag="U2R41_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM8888916.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U2R41_001496"
     CDS             7901..8038
                     /locus_tag="U2R41_001496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888917.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="U2R41_001497"
     CDS             complement(8851..9354)
                     /locus_tag="U2R41_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8888918.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="U2R41_001498"
     CDS             9713..10738
                     /locus_tag="U2R41_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8888919.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="U2R41_001499"
     CDS             11085..11963
                     /locus_tag="U2R41_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEM8888920.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="U2R41_001500"
     CDS             12439..12555
                     /locus_tag="U2R41_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM8888921.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="U2R41_001501"
     CDS             13483..13821
                     /locus_tag="U2R41_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888922.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="U2R41_001502"
     CDS             13915..14145
                     /locus_tag="U2R41_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888923.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="U2R41_001503"
     CDS             14545..14916
                     /locus_tag="U2R41_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEM8888924.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="U2R41_001504"
     CDS             complement(15190..15882)
                     /locus_tag="U2R41_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8888925.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="U2R41_001505"
     CDS             complement(15885..16670)
                     /locus_tag="U2R41_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8888926.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="U2R41_001506"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="U2R41_001506"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM8888927.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="U2R41_001507"
     CDS             18332..18649
                     /locus_tag="U2R41_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8888928.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="U2R41_001508"
     CDS             18662..20335
                     /locus_tag="U2R41_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEM8888929.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="U2R41_001509"
     CDS             20349..21980
                     /locus_tag="U2R41_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEM8888930.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..24042
                     /locus_tag="U2R41_001510"
     CDS             22852..24042
                     /locus_tag="U2R41_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEM8888931.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24176..26365
                     /locus_tag="U2R41_001511"
     CDS             24176..26365
                     /locus_tag="U2R41_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8888932.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26362..>26940
                     /locus_tag="U2R41_001512"
     CDS             26362..>26940
                     /locus_tag="U2R41_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM8888933.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVN"
BASE COUNT         8605 a         4476 c         5328 g         8531 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    24001 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    24061 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    24121 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    24181 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    24241 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    24301 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    24361 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    24421 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    24481 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    24541 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    24601 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    24661 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    24721 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    24781 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    24841 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    24901 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    24961 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    25021 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    25081 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    25141 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    25201 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    25261 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    25321 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    25381 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    25441 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    25501 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    25561 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    25621 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    25681 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    25741 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    25801 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    25861 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    25921 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    25981 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    26041 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    26101 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    26161 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    26221 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    26281 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    26341 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    26401 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    26461 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    26521 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    26581 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    26641 ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga
    26701 tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg
    26761 aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt
    26821 aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa
    26881 aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac
//
LOCUS       DASHWH010000034        26487 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.034, whole genome shotgun sequence.
ACCESSION   DASHWH010000034 DASHWH010000000
VERSION     DASHWH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.034"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..186
                     /locus_tag="U2R41_001513"
     CDS             <1..186
                     /locus_tag="U2R41_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888934.1"
                     /translation="FLAPGILGVVHVLFGLQMFGLFMQNPYKDIWVPFTIFFVLYFIY
                     YVLTTWLYTGIVLKKRL"
     gene            463..1503
                     /locus_tag="U2R41_001514"
     CDS             463..1503
                     /locus_tag="U2R41_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8888935.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1555..3699)
                     /locus_tag="U2R41_001515"
     CDS             complement(1555..3699)
                     /locus_tag="U2R41_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM8888936.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(4056..5429)
                     /locus_tag="U2R41_001516"
     CDS             complement(4056..5429)
                     /locus_tag="U2R41_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8888937.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(5429..5824)
                     /gene="rbsD"
                     /locus_tag="U2R41_001517"
     CDS             complement(5429..5824)
                     /gene="rbsD"
                     /locus_tag="U2R41_001517"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEM8888938.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(5852..6769)
                     /gene="rbsK"
                     /locus_tag="U2R41_001518"
     CDS             complement(5852..6769)
                     /gene="rbsK"
                     /locus_tag="U2R41_001518"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEM8888939.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(6877..7890)
                     /locus_tag="U2R41_001519"
     CDS             complement(6877..7890)
                     /locus_tag="U2R41_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8888940.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(7971..8570)
                     /locus_tag="U2R41_001520"
     CDS             complement(7971..8570)
                     /locus_tag="U2R41_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8888941.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            8756..9457
                     /locus_tag="U2R41_001521"
     CDS             8756..9457
                     /locus_tag="U2R41_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888942.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            9467..12178
                     /locus_tag="U2R41_001522"
     CDS             9467..12178
                     /locus_tag="U2R41_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888943.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            12629..14257
                     /gene="treC"
                     /locus_tag="U2R41_001523"
     CDS             12629..14257
                     /gene="treC"
                     /locus_tag="U2R41_001523"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEM8888944.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14317..15498)
                     /locus_tag="U2R41_001524"
     CDS             complement(14317..15498)
                     /locus_tag="U2R41_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8888945.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            15750..17273
                     /locus_tag="U2R41_001525"
     CDS             15750..17273
                     /locus_tag="U2R41_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM8888946.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(17342..18070)
                     /locus_tag="U2R41_001526"
     CDS             complement(17342..18070)
                     /locus_tag="U2R41_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8888947.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(18083..19363)
                     /locus_tag="U2R41_001527"
     CDS             complement(18083..19363)
                     /locus_tag="U2R41_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8888948.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(19504..20106)
                     /locus_tag="U2R41_001528"
     CDS             complement(19504..20106)
                     /locus_tag="U2R41_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM8888949.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            20351..20557
                     /locus_tag="U2R41_001529"
     CDS             20351..20557
                     /locus_tag="U2R41_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888950.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            20869..21588
                     /locus_tag="U2R41_001530"
     CDS             20869..21588
                     /locus_tag="U2R41_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8888951.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(21669..22352)
                     /locus_tag="U2R41_001531"
     CDS             complement(21669..22352)
                     /locus_tag="U2R41_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEM8888952.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            22570..24756
                     /locus_tag="U2R41_001532"
     CDS             22570..24756
                     /locus_tag="U2R41_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM8888953.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            25102..25512
                     /gene="spx"
                     /locus_tag="U2R41_001533"
     CDS             25102..25512
                     /gene="spx"
                     /locus_tag="U2R41_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM8888954.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            25526..25882
                     /locus_tag="U2R41_001534"
     CDS             25526..25882
                     /locus_tag="U2R41_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEM8888955.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8869 a         4698 c         5195 g         7725 t
ORIGIN      
        1 ttcttagcac caggaatctt aggtgtagtc catgtcttat tcggattaca aatgtttggt
       61 ctattcatgc aaaatccata caaagatatt tgggttccgt ttacgatatt cttcgtattg
      121 tatttcatct actatgtact gacgacatgg ttatatactg gaatcgtatt gaaaaagaga
      181 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agctcctcat
      241 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
      301 aaaaagaggt tgtgacaata tttttgtcac aacctttttt tcacggtctt ttattagaat
      361 atagtaaatt ttttctcgtt actcacggaa taagctgtta agaaaacggt ttccatgtat
      421 actggaatca gtgcaaatga aagaaaagag ggtgcaagga acatggaaat caatgaacaa
      481 aattttggcg aaaactatca cttgattacg atcacgaatc aaaatgggac atctttgtct
      541 gtcagtgatc ttggggcacg ggttgttcat tttcaaacaa taatcgataa tgaaaaacga
      601 gagttgatct taggttttga tgaagcaaaa gaatatctag aaaaagaccc ttatatcggt
      661 gcttcaatcg gacgtatggc tggacggatt acgaatggca cattcaaatt agatgatgaa
      721 acgttcgaag taaaaacaga cccggacact ggtcattgtt tacatggtgg tgcacctagc
      781 tttgaagcca agcagtggca ttatacagtc atcaatggtg aaaacgaagc atcggtcatt
      841 ttctatcaga ctagcccaga tatggaaaac ggatttcctg gaactttaga tgtagaagta
      901 cggtatacgc tgacgaacga tgatacatgg cgagtgacgg tccaagggat cagtgaccgc
      961 aaaacagtat tcaatcctac aaatcatgtc tatttcaatt taaccggtga tccagcacag
     1021 tcaatcgacg aacatatact ttggttgaat agccgattat ttgctacgct caataaagat
     1081 acaacgccta caggagaagt agtttcagta agtggcacag catttgactt ccaacgtcct
     1141 aaaaaattag gtgaggtctt tgccagtgat tttcgccaat caatgatagt gggcggcatc
     1201 gatcatccat ttttgttgaa aaaacaatct ggtttactaa atgctgcgga attgacaagc
     1261 ccagatgaaa aagtatcgat ccaagtaaaa acagatgctt caggaatcgt gatttttaca
     1321 gcaaattttg gagaagatgg accggaaatg cgcgggaata aattagtaaa tcatggcggt
     1381 atcacatttg agactcaaga actgccagga gcggagcgac ttgaaagttt tggagatgtc
     1441 actctggaac ctaaccaagt acgcgagttt gtaacggaat acaaaataaa tataaagaaa
     1501 tagaataaat taaaaaggga gtgtgacagt gtcacatccc ctttttaatt tattctattt
     1561 ctttaaaggc agatcaagcc agaatgattt gtctaaactt aacaaggtta gtacaggaac
     1621 agttgcagct ttaactgtac gagtagttgt ttctggtatc tgtcttgccg taaaattgtt
     1681 ccacaaatcc cagattgaag cgacacggaa gatggaagaa gcgttgttga cgcgttttat
     1741 cgttgaaaat tctaatcctg tgattggcac gccatctgtt gtccatttga ttggcacatc
     1801 gaatcgctta gacatattga ggatttcagc gtttgcttct tcagttgttg ctgcttcttt
     1861 tttcggttca tcatattttg tcaggtcgta cgcttcgatg atcgcgttca atttttgtga
     1921 atattggcga tctgtagcat aacgtccttc caaatatttg gttgcttctt tgtagtcttt
     1981 tgtttccgac ttccaagttg gtttgtagaa atcaggattg ccagtcagtc catttttgat
     2041 caagtcggca tagtcttcca aactttcctt gtaagaagga tatttacgga attgtgcgcg
     2101 gatttggtag ctttgtccgg aactatcttg ttcaagtgta ttgaaggtca cgctgcttcc
     2161 tttatatgat cctttgatac caaaaaggtt atgatttggt tcgcttgata ataagctgtt
     2221 tccagatcca ctttcaagga tagcttgagc catcatgact gatgcgtaga ttccttcttt
     2281 ttgcccgatt tcctgtgcat catctgcaat ctttttcaag aactgatcag ttgtttggtt
     2341 ttgactgaaa tggaacgagc catctaatcc atctaaaata gatgagccgc ctgtcagcga
     2401 acctgaagca gcaggtaaac ctgctaagtt tactcggaca gcaaaatcag gcgtgaaatc
     2461 tgaaatagaa gttactcgaa ctttatctcc tggttgaggc gcatggataa attgtccgtt
     2521 cccaatatag atacctacat ggtgagtaac accggaagaa ggtccccaga agacgaggtc
     2581 gccaggttgg gcctgactta ctggaatctg cgtacctgca tattgctgtt cgcctgtcca
     2641 gccaccgata taatgaccgg ttgctttcat atatacgtaa taagtcaaac cagaacagtc
     2701 aaaagtattt ggaccttttg ctccccatac atatggtttt ccaagatgct tgaatgcttc
     2761 tcgaacgatt gcttgttgga cattcgttgt atttgtaggc aatacagcat caattggtga
     2821 ttttacaaca aaatcagaag aagcagaaga tggattctct ataccgcttt gatttgctgt
     2881 atttggacga gatgaagcat tgttcgttcc gccattcgat gaaccattgt ttggatggtt
     2941 tggttctctc tgagtatttc cattgttatt cgatccagat gggtttgttt gattcgaaga
     3001 attcccgctg ttcgaattat tgttattatt gtttgaatta tttgaaccat tattattgtt
     3061 gttattaccc acatctggtt tgtttggcgt ttccggatct ggtgtactag ggacttcttc
     3121 actaggatca gttggtttct ctgttgaagt atccggtcca gctttaaatt ctgcagtaat
     3181 agataaattg ttcgtaccta ctgtaaaggt ctttgcatca taatttactt ctttggcacc
     3241 attaacagcg actgaagcaa tttgttcacc ttcagctggc gtaatagtgt aagttacctt
     3301 cgtttccgct aataaacctt ctaccgttcc actttccgta atatccaaag gttgctgttc
     3361 aggggaatcc gctgtcgaag cagtcagttg gatcttacca atcaaagaag gagcaattgt
     3421 aagggaatat gtctcggctg gcttttcatt ttctgatgga tcagccggat caatttcagt
     3481 tggctcggtc gatcctgttg ttgagttagt tgttccagtt gggtcttctg taaaagaatc
     3541 cgttgtttca ctagtagctg agctggatgt agtcgtatct gttgtgactg tttgatcagc
     3601 tatttgatta tctacggcaa atacggtatt ggtcgacaaa acagacggag caatcagcca
     3661 agtagcagct agcaaacgta tcgttttttt atttttcata ataaactagt ccttttcctt
     3721 tcattgattt tctgtcttat tcagcattca ttagacattt tttggaacag ataatcgtgt
     3781 cgaaaagctt tttcttttca taaatctgtc tgtgtattct aaataaaaca atttgtaaat
     3841 aagcaacgag cattcattcg tttttctttg acgttcattt tattgcatag accacctttg
     3901 tttggggata aacaagtggg gataaaaatg gttatgcata tgtaaattat accaaagttt
     3961 ggaaagtatc acaaaaattt ataaaaataa tgatcctaaa aagaaaaaga ctgaaatatg
     4021 tgataaacat ctcatacttc agtctttttc attaactatt cataagacat taggtcttca
     4081 tcaaacccgc taacgagagt agtgatcatt gctacgacta gactaacagc cagtcctgct
     4141 aagtaccaga aaaaagtacc ggaaatgaat gcaggcaatg tggtcagact accaaataca
     4201 aaagcgatcg ctttcacttg ggcaaatccc attacagctc caccagcagc tgcaccaatg
     4261 caagcagcaa caaatggttt tttcaatggg atcgcaattc cgtaaacagt tggttcggtc
     4321 actcccgcaa gtaaaatcga agcaaaagca gaaccggaga tcgaacgggt ttttgaacgt
     4381 ttacttcgca gaaatacacc taaagctgca ccagccattc caaaattggc tgctcctgtc
     4441 aacggaacga ttcggtcgaa cccataagca gagatattac tgatcatgac agggtttaca
     4501 gcccattgga taccaaatat taccaaaata ttccaaccac cagcgatgat tgcgccactg
     4561 atcggtccgt taatatccat catccaagta attgcggcag ctaaaccttc gccaaggtaa
     4621 acacccgcag gcccgaatac cattaacgtc aaaggaaccg tgataactaa tgaaagtaaa
     4681 ggtacaaata ctaattgcat attttcgcga atatattttt tgaggaattt ttcaacaaaa
     4741 gaataaaaga aaatacctaa aatcgccggc atgacagtag acgtatactg catcagtact
     4801 acaggaatcc ccagaaaatc agtagctgct ttgccactgt ctgaaagtaa agaggtcaga
     4861 ttcggttcaa tcagtgctgc agcaatgaca gccgcaatat atccattcac tttaaacgtt
     4921 ttggcagcag aaaaagcaag aaagattggc agaaaataaa agacggcatt cgacgcagca
     4981 gacaaaatat gataagttcc gcttgtttca ctaagccagc caatcgtagt cgctaaaaca
     5041 accagacctt ttaatatacc agaaccggca aacagcggaa tgatcggcgt aaagatttcc
     5101 gatatcaaac caaatagttt ttcgatcgga tttagctttt ctttcggagc tgaatgatcg
     5161 atttgacgcg tcaattcctt gtaagcagga gccaatccct gatccatgat tatttctgca
     5221 tatctatcga gtaaaatgaa atacttaatg gaagggattt gctgttttaa tttttctgca
     5281 ttggctactg gctgtttttt caatttgata cgaagccgag tcgcacatgg aaggacagaa
     5341 tcaatattgt cttttcctcc caactgttca atcagcatat gaatctgctc ggacagtacc
     5401 atctgttcag gattcattgt cgtttccatt agaagaccac tccactcact aatataatat
     5461 tggcgtaagg tgtcatttcg cctgtacgaa taaatgcatg acagttattc aactcttttt
     5521 tcatttcttc atgtgggata aagctaatcg gtgtatctgg taacaataat ttgatttttt
     5581 ccaatatttc cgggttcatt gtctttattt cttccgccaa ttcaatcgat tgtacctcaa
     5641 gttcttctaa aacgttttct aaaacagata aaaaacttgg aagaccgttt gagacagcca
     5701 aatctatttt ttcagtacca aaggggacag gcatgcctgc atccccgata cttaatttat
     5761 caaagtgccc catttgtgcg attactcgtg aaatatctga attgattact tttccttttt
     5821 tcatgtttgt ttcttcctct cattgttaat tttagactgc tgattgttct aaagcttcgc
     5881 ggtaagggat agaaggttgc gcaccataag actgcacagc aacagaagag gctagatttc
     5941 cataaaggat cgcttctttc aagttactaa ggtctggctg aagtgtgctg cttaatgcgc
     6001 caataaatgt atcacctgcg gctgttgtgt caacggcatc cactttaaaa gcagggacga
     6061 taccgtgttc tttttctgta tgatagaaag cgccggcact gcctagtgta atgattactg
     6121 ttccaatacc taattcatgg agtttttcgg ctgctgcgac caaactgtta tgatctgtca
     6181 ctctgattcc tgtaatgatt tctgtttctg tttcatttgg tataatgata tctgtctttt
     6241 caagtagttc agtaggaatc gtttcacgag ctggtgcagg attcaggatc gttgtttttc
     6301 ctgcgtcttt tgcaatagaa aaagctagga tcgtcgcatc taagctactt tcaaactgag
     6361 caataataaa gtcactgttt gtaatcagtt gcttgttttt taggacttct tgttcatgga
     6421 aagcattatt tgcccctgca tggatcaaga tactattttc tcctgctgcg tctaccataa
     6481 tcattgcttg tccggttgtg gcgccttgga tttcagctat cccagatgta tcgatgtttt
     6541 cttttgtcag tagatctaat aactgttgtc cttcgctgtc agccccaacg cctccaataa
     6601 aagatgtccg tgcgcctgaa cgtttggctg ccactgcttg gttggcacct ttacctccgc
     6661 cagcgtgaaa aatctcatgc gcatgcatcg tttcgcctgg cttgggcatg ttggttaaac
     6721 gaagggtagt gtccatatta atacttccaa ttactgtaac cgtgttcatt ttacaaacct
     6781 ccaactatct catttatttt gatgggaaaa ttatttttta aaacgtttta gtaaaacgtt
     6841 ttaatagttc gataaaaata tagcacacct tgaaagctat ttcaagggtg cagttgaaaa
     6901 tcttttttct aatcgtacag gtaatctttt ttcttcccat tctttttctg ggaattggat
     6961 ccgatctaac aataacttgg cagatgtttg tcccaattcg aagattggtt gtgcgatcgt
     7021 tgtcagtttc ggcttgatat actcgcacat atctatatta tcatagccaa taatgctgta
     7081 gtcttcaggg atgctttttc cagcttcctc taatccgcga tataatccaa aagctaattc
     7141 atcatttaaa gcaaacacac cagttgcagc agaatcaaga agttctgctg tgatctggta
     7201 gccaccttgt tttgagaact gtgtaggtaa gaggatcagt tggtcgtgag ggatttggta
     7261 aacatcaaga gcagatttaa acccctcaat ccgagcgtgg acattttccg gtggattttc
     7321 aggatagaca agggcgatcg tttgatgtcc tagactaagc aagtgcatgc ccgctaggta
     7381 accaccgcga aaatcgtctg ttcgtacggc atcactaaat ccttcagatt ttttctgatc
     7441 caaaacaatg aagggccggc cttgtttttt caaattttcg ttgattgcat ctgtagagac
     7501 agcagaagtc gcgataatga atccatccac acctcgacgt gtgagctcgg ctagatattc
     7561 gatttctttt tgatgatcag aatcagcatt acataatatc gtgacgaaat tctgcttata
     7621 caagatatcc tcaattcctc gcatcagtgt actgaagaat ggattcgtga tatcaggaac
     7681 gagtacacca attgttttcg tttccttggt aatcatttgt ctagcaaaat agtcaggctg
     7741 atagcctaat tcttctttgg cagctaaaac tttctgaaca gttttcggac tgaatttctc
     7801 cgtatttcca tttaatatca aggagacagt tgtgattgaa acgccggaaa gtttagcgac
     7861 ttctttgatt gtgatctttt ttttcaccat gttcatactc ctatctaaat cctgtggtta
     7921 ttttcccatg aaatcaaaaa gaagtaaatg aaaatcacgt gtgccagtac ttatttgctt
     7981 tgttggatag cttcgattat ttgtttcgtt gagcgtactc gtgcagtttt cggaaatatc
     8041 ttttttatga acaattgatg ggattctttg tcacgatcac tgcaagcatc ttcagcaagc
     8101 acgatatggt acccgtgttg aaaggcatct aatgcagtag catatacacc gtttgttgtc
     8161 gctacaccgc ttaaaatgat agtttcgatt ccacgccggc gaagttgaag atcaagagaa
     8221 gtaccaaaaa aagcacttgg attgtatttt gtgatagtca acatattatc tgtatctttc
     8281 aaagaatcct tcaaaagtaa atcagtgaat tctggaggta cagaagcagt cattccaccc
     8341 atatcagttt gttgactcaa ataagggaaa gtggagatgt ctacgttcac taaaactttc
     8401 aaggcagcgg tacgtttcaa tgttcttact aacaaatcat tgtttgtcag aatagatgct
     8461 gcagtgtgtg gagagagtgt accgttattt acgatctctt tttgcatgtc gatcgcgaca
     8521 aaagcagttt ttgataaatc aaaaatcata ttttccttta aatcattcat gatgttcctt
     8581 ctttcaaata acaatgtttt ttatgtaagt aaatcataaa tcgtttattt gtcaactaaa
     8641 ttaaaggatt cctttcttta gtctagagaa attcagtggt aaaataaata agtgtgagaa
     8701 aacgcattcg atgcatagat atagcgagaa atatcgtgaa aatggaggaa tcagagtgaa
     8761 gtatgtcgaa gtaaaaaatg aatacaaacg atacaaaatg ggagaaacga ccattacagc
     8821 aaatgacgat atttcttttt caattgaaaa aggcgaatta gtgattatac ttggtccaag
     8881 tggagcagga aagtcaacga ttcttaatat tttaggtggg atggatacac ctgatgaagg
     8941 tcagatcatt attgatgaca cagatattgc gcaattttca gataaacagc ttactgctta
     9001 ccgacgaaca gatgtagggt tcgttttcca attctacaat ctcgttccta atctaacagc
     9061 aaaagaaaat gtggaattgg caacggaagt ttctcctgat gcgcttgatc ctgtagaggt
     9121 attgaggcaa gtgggcctag ctcatcgatt gaataacttt ccgtcccaat tatcaggagg
     9181 cgaacagcag agggtttcaa ttgctcgtgc actagctaaa aatcccaaac ttcttttatg
     9241 tgatgaaccg acaggcgcgc tggattttga aacgggcaag caagtattga aattattgca
     9301 aaacgctagc agacaacacg gcaataccgt tttgatcatt acacataatt ctgctttagc
     9361 gcccatcgct gatagagtca ttcacattaa tgatgctaaa gtaaggtctg ttgaattaaa
     9421 tgatcatcct tcatccattg atgagattgt atggtagggg gattttatga aaacaagaac
     9481 atatcttaaa gcaggttttc gcgaaattcg gcaatctaaa ggacgattca ttgcaatcat
     9541 ccttattgtc atgctaggaa cacttttatt tgtaggtgtc aaaactgctg gacctgtcat
     9601 gcaaaaaacg atggatcact atgttggtac agcaggactt tctgatttgc aaatcgtttc
     9661 tactggtggc ttgacacaaa aagatattgc tcaagcagaa aagatcccag atgcaaaagt
     9721 agaaacagga aaacagatct attattccaa tactggcaaa aacgaagtga ttcaaatatt
     9781 ttcttataac aaaaataaca agcagaacaa gctacaatta actgatggaa aactcccgga
     9841 aaaaccaaac cagcttgtat tggatgaaaa agcggaagat gaagggtata aaatcggtga
     9901 tatttatcag attgattcag atgaattgaa agaaaaagag tataccatca cgggctttgt
     9961 ccgttctcca ttgttcatca acaatttgga gagaggatac gcgaacgtcg gaaatggtag
    10021 cgtcgattat tttgtctatt tacctgaatc cgtttttaaa acagatattt attctgtctt
    10081 atatcttgat tttacaaacg taaagaattt gaatacgtac agcaaagctt acaaagataa
    10141 aatggagcaa aatcaagaaa aggcagaaaa gtatttacaa gatcgacccg aagaacgctt
    10201 agaagagctg aaaaaatcgg ccaacgaaga attagaaccg gcacagcaaa aaatagcgga
    10261 tggcaaagca caaacaacgc aagcaagaac acggttagaa gatgctaaaa aacagttagc
    10321 gcaacagtca gcaactatcc agcagcttcc tgaagcgcaa cgaacaatcg ctgttcaaac
    10381 attatctcaa caagaagctc aacttgctga acaagaaagg caattagttg agaaagaacg
    10441 tgaactggcc tctgcagagg aagaattgaa tcgaaatgca gaaaaaattg aaaatttaac
    10501 taaaccatca tacctataca atgaacgttc agaaaatgtc ggttttcaag aatttggtga
    10561 tctgtctgat cgtatcgctg cgattgccaa tgtttttcct gttttctttt tctttattgc
    10621 cgcattgatc acctttacga caatgacacg tatggtagaa gaaaaccgca gggaaatcgg
    10681 gacattaaag gcgttaggtt atacgaaagt tgaaatcgca aaaaaatatg caatctatgc
    10741 aagtctagcc tcagggatag gtattgtttt aggaacgata ttaggaacga atcttttgcc
    10801 aaggatcatt tttgagctgt ctaacgagcg gtatgatatt gggagtgcgc ttatttttta
    10861 tgactggccg ccgattttac aagcagctgc cgcattcttc attgccgcat ttggcgctgc
    10921 aatgatcgtt ttattcaaag atttaagaga acggcctgct gcattgcttc aaccgaaagc
    10981 accgaagcca gggaaacgca tttttcttga atacatcact ccgctgtggt cacgccttag
    11041 ctttaatcaa aaaatcagtt accgaaatct cttccgatat aagtcaagaa tgcttatggc
    11101 gattatcgga attgctggat gtgccgggct gatggttgca ggcgtgggat tgaaagattc
    11161 tctaagttct gtttcagcca aacaatttgg accgatcatt gactatcaag caatcgttac
    11221 attggattca tccacggaag atagtcgtca aaaagcagcg gatatgctgc agaagcaaga
    11281 aaaagtaaat gagatcctag ctattcatac tcaaaccgta gaattgagaa aaaaaggaca
    11341 tgcttcgcaa agtctgacag tgatcgtacc aagtaaaaca gctgatttgg atcgttttat
    11401 ccatttaact gatcttgaag agaacccacg ctccttgcct aaaacaggaa tcgctattac
    11461 gcaaaaagta gcagaactat ttgacttatc tacaggagat catatttcgc tttatgatga
    11521 agatcaaaaa gaatggaaag tcgaagtaaa agaaatcgtg aaaaactatc taggtagctt
    11581 tatttatatg tcccctgaat attatgcttc agttactgat caatcgatga tggataatgc
    11641 atttttgctg cgtacagata agatgacaac aaaagaagaa gaaatgcttt ccgagaaact
    11701 gcttgccaca gacgctgtga caaatacgtc tttcatgtcc aaacaaatcg aaacgcaaga
    11761 agaatcaatg gcgaacttag actcggttgt cttaatcttt gtcgtattat caggactgct
    11821 ggcttttatc gtcttgtata atctaactaa tatcaatatt tccgagcgtg tacgcgaatt
    11881 atctacaata aaagtccttg gattttttga taaagaagtc acgatgtaca ttgttcgaga
    11941 aaatatcatc tttactcttt taggaatcat cggaggattt ggtgtaggat atatattgac
    12001 tgacttcatc ttgcaacaag cgtctatgga gaatgtcatc tttccattag tgatcacatg
    12061 gtgggcttat gtcttatcag caggattaac tatcgtattt acggttattg tgatgattgt
    12121 tacccatttc aaattaaagc atatcgatat gattgatgcg ttgaaatcga atgaataaaa
    12181 acagaggttg tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gcaaaatagt
    12241 ttttcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acaccatgaa
    12301 tcagaggttg taaaaaagcc gtcctgcatc gagcaaaaag aatactcaaa taaaattagc
    12361 tcctcatgtt ttacatttga gaagactgat tgccgtagat aaaagctttt gaacaccatt
    12421 tatagaagaa acaagaatat tttgctattt taaacaagag gatagacaaa tacatgtctg
    12481 atctcttgtt ttttattggg caacagacct cttagaatgt ataaagataa attttacaaa
    12541 cgctttcggt tcagttataa taataaatga taacttgtat atataagttg tggtaggtaa
    12601 taaatcagtt gaatggaggt aaatatccat gacatttaaa gacaaagtca tttaccaaat
    12661 ctatcccaaa tcttacaaag acagtaatgg tgatggaatt ggagatttaa aaggaatcaa
    12721 agaaaaactc ccgtatctga atgatctagg catcgacatg atttggctaa accctattta
    12781 tccaagccct caaaaagaca acggttatga tatctctgat tacacagcca tcgatcctat
    12841 ctttggtacg atggaggaat tcgaacagct aatagccgaa gcaaagaaat accggataga
    12901 aatcatgctc gatatggtgc tgaatcacgt ttcgacagaa catgaatggt tcaaaaaagc
    12961 actagcaggg gaccagcatt atcaggactt cttcatttta agagaagagc caacggattg
    13021 ggtatcaaaa tttggtggaa gtgcttggtc gccatttgga gatacgggga aatattattt
    13081 gcacttatat gatcggacac aagctgactt gaactggcgg aaccaagcag taagagagga
    13141 actattcaaa gttgttcgtt tttggatgga aaaaggggta aaaggattcc gtttcgatgt
    13201 tatcaatgtt atcggtaaag acgaacaatt gaaaaataat aaagaaaatg atggcaaacc
    13261 tgaatatacg gacaaaccaa tcacccatac gtatctaaaa gaattgaacc aagcaacgtt
    13321 tgggacggat tccactatca tcacagtcgg tgaaatgagc tctacgacga tcgaaaattg
    13381 tatcttgtat acagaaccta gtagagaaga attatcaatg gtctttaatt tccatcattt
    13441 gaaagtcgat tatgaaaatg ggaataaatg gtctactcct ccatttgatt ttgcagaatt
    13501 aaagcgacta tttcatacat ggggagaaaa gatgagtgag ggtaacggct ggaatgctct
    13561 attttttaat aatcatgatc agccacgtgc gctcaatcga tttgtagcta tcgatcagta
    13621 tcgaaaacaa ggagcagaga tgctagcaac catgatccat ttgaaccgtg ggacgcccta
    13681 tatttatatg ggagaagaaa tcggcatgat cgacccagac tattcatcga tttccgacta
    13741 tattgacgta gaaagtttga atgcttataa gttactcctt gaacaaggat tcacagaaaa
    13801 agaagcgttt cgtcggatca aagcaaaatc tcgcgataac tctcgaacac ccatgcaatg
    13861 gtcagacaca gaacaagcag gatttactac agggaaacct tggttaaaag ttggaggcaa
    13921 gctagaagag attaatgtag aaaaagaact atcttctgaa gattctattt tgtcctatta
    13981 caagaaattg attcggctga gaaaaacata tccaatcgtt gcacaaggag attatcatgc
    14041 ttatgaagca aatcatccgg aagtttatgg ctttcttcgc caatttgaag gacagcagct
    14101 gcttgtatta acaaatttct atgataaaga tacaaggatc gccatcccag aaggattcac
    14161 agaagccaat attcttatcg ataattattc cgatactgtt cttcatcaaa gtctggagct
    14221 aaaaccatac caggcaattg ctttgctgaa aaattaaaac aaaaacacaa aacgtcgttc
    14281 ctacgagcta ttctcgtaag atcgacgttt tgtgttttat ttaaaataat cttcttcaga
    14341 aaatgaagag ttttcatact ctcctttatc gccataaaat ttattgtagc gttgtttgaa
    14401 tttacggaaa ataaaagtaa cgagtaattt gactaacgta tagataggaa taccaaaaat
    14461 aacgcccaca attcccatta gatcacccat gacaagtaaa acgattaaga tcgtaatcgg
    14521 atggatctgt aaacgatcac ccattactcg aggaatcacc aagtcaccgt ggagaagttg
    14581 tacggcaaac cagacaatga caaatttgac agccataaaa gtagagtctt ggaatgcaat
    14641 gatcaaacct ggaaagaagg cgataaacgg cccaatatat ggaatgatac ataacaaccc
    14701 agcagaaata gctaaaacac tagcaaattc taatccgatc agtaagaaac aaacccagta
    14761 gactgctcca aggataaatg aagcaatgat ttgccccttt agaaatgaac ccaattgttg
    14821 attagcaatc tttgtcaaat ttttaaaatc tgtacgaaat gctggtggga caatcgctaa
    14881 aactgactga taaaattttt gaggatctct caaaaggaaa aaagcgacaa ttggaccagt
    14941 aaataaagta ataaaaatag ctgtaacagt tgaaaaaatg ttgctgatcc gttgtgcgcc
    15001 actttcccaa taatttccaa taaatccagc tatatcagac gtcacgtcgt caatagaagc
    15061 gaaaaattga ttaaatgaat cggcaaaagg tgttttctcc aaaaattgtc ggagactctc
    15121 ttgaaaatct tgaaacaaat ctggcaaact actgatcaat gtctttgcct gttcttcaag
    15181 aaaaggaata agccatatgc ttccaagccc caataaaaat aaaataatca cataaacgat
    15241 aatgactgac aagttcctcg aaaggtgctt gctcaaacgt ttgatgactg gttccaaaag
    15301 ataatacaag atcagtgcaa acaataatgg gggcaagata gtagcgataa tgattccgac
    15361 aggcttgaat ataaacgaga tctgatggaa aaaataaatg atcagcgcaa gtaagataac
    15421 tattcctaaa acatagtaag aggtttttcc tcctaaaaag cggatcaaac gattcgttgt
    15481 atctgtttct tttttcactc tttttcctcc tttatctata aattcattat agacaaaagc
    15541 gggtataaaa ggaaaatgat agaggttgat tgcaaaaata tcgtcaatca ttcatataaa
    15601 ctttctcatc aattaaaata agaaaacgct tgacagttct tttaaccata tgataaatta
    15661 aatcctgttg ctaaaacgta actattacgg tttgcgaaaa gcgaatcgga acattcaaat
    15721 gaaagaagat gagaagagga gtgtcataaa tgaaacgatt gataggtata ttggccatgc
    15781 tggttttagc aggtatgtta actgcttgcg gggcaagtgg gaaagccgag gacagtaaag
    15841 aaaaattgag cgtgatgacg accttttatc ccatgtatga ttttacaaaa gcaatcgtcg
    15901 gggatgaagg agaagtagag ttattgattc cagcagggac tgattcccac gattatgaac
    15961 cttctgctaa agacatggca aagatccaag atacagatat ctttgtttat aatgatgaaa
    16021 acatggaaac atgggtcccc gcaatccaaa aaacattaca agaaggcaat gtccatacga
    16081 taaaagcgac agaaggtatg cttttacttc cgggaagtga agaaggacat gatcatgacc
    16141 atgaacacgg ggaagaaggc catacacatg agctagaccc acatgtatgg ctagctccaa
    16201 gtttagcaat caagcaggta gcaaacatac gtgatcagct gattgaagct tacccagaaa
    16261 aacaggaagt atggacaaaa aatgcagcag cctataccga gaaacttcaa gcactccatc
    16321 agctttatca agaaaccttt aagcaagcaa aacaaagatc ttttgtcacg caacatgctg
    16381 cattcaacta tctagcgtta gaatatggat tgaatcaagt atcgattgct ggtctttctt
    16441 ctagtgaaga accgtctgcg gcgagaattg ctgaactgaa acactttgtt aaagaacatg
    16501 gaatcaacta tatttatttt gaagaaaacg ccaaagatag tattgccaga acgttagcaa
    16561 acgaagcagg tgtatcttta gaagtattga atcctttgga aggattgacg aatgaacaaa
    16621 tagaaaatgg agaaaactat ctttctatca tggaagcaaa cctcgaagcg ttgaaaaaaa
    16681 cgacagagac agaaaacccg ctagaagata ctttgacccc caaaaaagaa aagagcgtct
    16741 acaacggtta ttttgaagac gaggatgtac aagatcgctc tctttcagat tgggcaggaa
    16801 cttggcaaac agtagatacg tatgtagaag atgggacttt tgatcctgtt tttgaatata
    16861 aagaaaaatt gaatcaggat aagacagcac aagaatataa agaatattat acgaagggct
    16921 atcaaacaga tattgcttca atcaagatca gtgagaaaga gatggcattt acgtttaaga
    16981 atggaacaac gaaacgttca aattatcgtt atgtagggaa agaaatcttg acctacgagg
    17041 caggaaaccg tggcgtaagg tatctattcg aagcagaaga taaggaaagt ggcgctttta
    17101 gatatattca attcagtgat cacacgattg caccggctga ctccgaacat tttcatttat
    17161 atgcaggaaa tgaaagtcaa gaagtattgc tgaaagaaat ggagaattgg ccaacatttt
    17221 atccagatac aatggatgga cgtgaaattg cacaggaaat gcttttgcac tgataggcaa
    17281 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
    17341 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
    17401 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
    17461 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
    17521 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
    17581 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
    17641 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
    17701 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
    17761 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
    17821 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
    17881 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
    17941 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
    18001 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
    18061 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
    18121 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
    18181 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
    18241 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
    18301 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
    18361 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
    18421 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
    18481 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
    18541 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
    18601 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
    18661 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
    18721 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
    18781 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
    18841 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
    18901 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
    18961 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
    19021 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgc
    19081 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
    19141 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
    19201 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
    19261 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
    19321 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
    19381 gatttttacc aaattatagc acaattggcg tattattttt ttaatttttc agggattttg
    19441 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
    19501 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
    19561 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
    19621 agctgagaaa aataacgtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
    19681 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
    19741 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
    19801 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
    19861 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
    19921 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
    19981 tagttttcca agattattca ataaaacttc taagacaaat tttacttgga catacaccat
    20041 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
    20101 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
    20161 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
    20221 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
    20281 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
    20341 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
    20401 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
    20461 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
    20521 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
    20581 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
    20641 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
    20701 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
    20761 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
    20821 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
    20881 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
    20941 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
    21001 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
    21061 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
    21121 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
    21181 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
    21241 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
    21301 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
    21361 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
    21421 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
    21481 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
    21541 tacactacaa ataacccgaa agcaaaggga atctttacaa ctaaataata taaaaactaa
    21601 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
    21661 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
    21721 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
    21781 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
    21841 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
    21901 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
    21961 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
    22021 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
    22081 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
    22141 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
    22201 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
    22261 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
    22321 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
    22381 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
    22441 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
    22501 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
    22561 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
    22621 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
    22681 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
    22741 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
    22801 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
    22861 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
    22921 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
    22981 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
    23041 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
    23101 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
    23161 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
    23221 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
    23281 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
    23341 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
    23401 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
    23461 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
    23521 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
    23581 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
    23641 aaaactcttt tcttattaac taattatcgt tctcgggcag ctattgtcca aggagcaaat
    23701 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
    23761 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
    23821 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
    23881 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
    23941 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
    24001 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
    24061 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
    24121 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
    24181 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
    24241 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
    24301 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
    24361 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
    24421 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
    24481 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
    24541 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagta
    24601 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
    24661 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
    24721 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
    24781 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
    24841 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
    24901 gacaagaatt ttgtcacacc tcccttattt attttgaaaa tgagaatact attatcaaaa
    24961 tagagaatat atagaaaaat tctcattatt caaacgtaaa taatcagtta ttttgatttt
    25021 ttttatattt tattagctaa taattggtat tttaaaagaa attactgtag aattggatta
    25081 taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
    25141 agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt
    25201 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
    25261 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
    25321 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
    25381 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
    25441 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
    25501 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
    25561 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
    25621 cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
    25681 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
    25741 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
    25801 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
    25861 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
    25921 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
    25981 ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata
    26041 tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt
    26101 aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta
    26161 gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct
    26221 atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga
    26281 gttgctgtaa gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta
    26341 cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac
    26401 tcctgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
    26461 tcttgattca attcaagaca acaaaca
//
LOCUS       DASHWH010000035        26028 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.035, whole genome shotgun sequence.
ACCESSION   DASHWH010000035 DASHWH010000000
VERSION     DASHWH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.035"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="U2R41_001535"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="U2R41_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEM8888956.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="U2R41_001536"
     CDS             809..1243
                     /locus_tag="U2R41_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEM8888957.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="U2R41_001537"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="U2R41_001537"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEM8888958.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="U2R41_001538"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="U2R41_001538"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEM8888959.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="U2R41_001539"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="U2R41_001539"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEM8888960.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="U2R41_001540"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="U2R41_001540"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEM8888961.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="U2R41_001541"
     CDS             9514..10440
                     /locus_tag="U2R41_001541"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEM8888962.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10622..11515
                     /locus_tag="U2R41_001542"
     CDS             10622..11515
                     /locus_tag="U2R41_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEM8888963.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11649..11774
                     /locus_tag="U2R41_001543"
     CDS             11649..11774
                     /locus_tag="U2R41_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8888964.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11908..12540)
                     /locus_tag="U2R41_001544"
     CDS             complement(11908..12540)
                     /locus_tag="U2R41_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888965.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12688..13230)
                     /locus_tag="U2R41_001545"
     CDS             complement(12688..13230)
                     /locus_tag="U2R41_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEM8888966.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13365..13754
                     /locus_tag="U2R41_001546"
     CDS             13365..13754
                     /locus_tag="U2R41_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888967.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13758..14522
                     /locus_tag="U2R41_001547"
     CDS             13758..14522
                     /locus_tag="U2R41_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8888968.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14651..15598
                     /locus_tag="U2R41_001548"
     CDS             14651..15598
                     /locus_tag="U2R41_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8888969.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15728..16468
                     /locus_tag="U2R41_001549"
     CDS             15728..16468
                     /locus_tag="U2R41_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888970.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16759..17427)
                     /locus_tag="U2R41_001550"
     CDS             complement(16759..17427)
                     /locus_tag="U2R41_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8888971.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17561..17848
                     /locus_tag="U2R41_001551"
     CDS             17561..17848
                     /locus_tag="U2R41_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8888972.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18264..19802
                     /locus_tag="U2R41_001552"
     CDS             18264..19802
                     /locus_tag="U2R41_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEM8888973.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19799..20764
                     /locus_tag="U2R41_001553"
     CDS             19799..20764
                     /locus_tag="U2R41_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888974.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20751..22073
                     /locus_tag="U2R41_001554"
     CDS             20751..22073
                     /locus_tag="U2R41_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8888975.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22136..22240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22314..23057
                     /locus_tag="U2R41_001555"
     CDS             22314..23057
                     /locus_tag="U2R41_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8888976.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23072..24064
                     /locus_tag="U2R41_001556"
     CDS             23072..24064
                     /locus_tag="U2R41_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8888977.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24076..24813
                     /locus_tag="U2R41_001557"
     CDS             24076..24813
                     /locus_tag="U2R41_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8888978.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24803..25525
                     /locus_tag="U2R41_001558"
     CDS             24803..25525
                     /locus_tag="U2R41_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM8888979.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25609..>26028)
                     /locus_tag="U2R41_001559"
     CDS             complement(25609..>26028)
                     /locus_tag="U2R41_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEM8888980.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8953 a         4426 c         5430 g         7219 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa agagaacata
    10621 tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg atgatccgct
    10681 tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga ttctaagaga
    10741 attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat tgatcgatgc
    10801 aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat ttgatcctaa
    10861 agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat tgaagagact
    10921 ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt gtttcgaaga
    10981 tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag aattgctgag
    11041 gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag aacacttccc
    11101 gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt
    11161 caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg atcaaataga
    11221 agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta gtattttcca
    11281 tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat tagaattacc
    11341 tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt cattgacaat
    11401 ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga aatttatacc
    11461 gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa cctaagagag
    11521 cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct ttatggttaa
    11581 aagaattatt tgagtatttt gcctatctac cacctattag attcactaca tggtctggaa
    11641 acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta gtgataagaa
    11701 aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt ttatgggaag
    11761 aactagctga ttagattagg aagtccattt gataatccag aatcactaag aaacgaatgg
    11821 cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt gtttaaaact
    11881 aaaaaagcat ttatgaagtc gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt
    11941 ttttctttgc cacataaata cacctagact gacgatgaag agcatatcca acagatttcc
    12001 tgcgacttgg atcgtcgaag ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa
    12061 caagctgtaa ataaaacagg tgacaggaac taatcgaaag aagagcggac atttcaattg
    12121 gctaaaaatg agtgaaaaaa taaaataagt aaaaatggat aaaatcacga ctaaaccatt
    12181 catgataaag aagaaaagcg tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga
    12241 aaacgtttta aatgcaatca atacgcttga agcttcgttt aacgtaccag gatgtcgatg
    12301 aaaaaaatag gcaccgaaat aaacagaaaa cagtaagccg caaagagaag acaaaatcaa
    12361 aaaaaacgga ttttcttcgg cttctgtgat atcagaatga tccaaaagca accagcctaa
    12421 tgcactagaa ggcgcatgtg aggtcttaag gtatgaataa catagaaaaa ataagatcgt
    12481 caaagttgca aaaaaagagc ctaaatgtaa aaaattagtg gttctacgtt gaatttccat
    12541 aatatctcca aattaaattg ataagtatat aaagtataat aacaaattga tgagtatatg
    12601 tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa tttaatttag
    12661 atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca cttttttagt
    12721 ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac gctcttcgat
    12781 ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta tttcgagaac
    12841 tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac cgtgttctct
    12901 cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat
    12961 gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac ctactgcgta
    13021 acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa caagggataa
    13081 gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca gtcgatagcc
    13141 tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat taatgaggaa
    13201 gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt atataacaac
    13261 aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca aaaaaatgat
    13321 aaaatgatta taatagaatt ataaagatag gttaggtgat taaaatgaaa acggtcaatc
    13381 gtgtgttttg tgccagccac gtgctaagtc tccttctcag tatatactta ggtgtctatt
    13441 cgattcaaca gccaattgag gtagagaaca ttgtttttca gatgtcgctg acagatggtt
    13501 taattttaag cgtttttttc tttttgatac tgtttattgg taatattttc ggtagcgttg
    13561 ccagtttttt gaatttttct atttatccac tcctttcgct agcattagga gtggtgggac
    13621 tttgttctct gtgcttattt cccgagccat tcagtcctgc atttatatta tttggtacac
    13681 tgaacctgtt ccagacaact gttggagcat ggttgctatg gcgatcagga aatttaatga
    13741 aaataggtga gtaacaattg aatccaatta tattgaaacg ttctgtccat catattccag
    13801 tgttagaagt tgttctgaaa gaattaagag aggaacgtat tcccgttgtc atctattatc
    13861 acggatggca gacaagtaag gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg
    13921 gcattcgtgt gatcctgcca gatgcgatga atcatgggga gcgcaaacag ccggtttctt
    13981 ctattccatc ctatacattt tgggatagta tctacggaaa tctgtttgaa tttgacacac
    14041 ttatcaagca tttagaaaaa agaggtcttc tacatgatca gcttgcagtt ggcggggttt
    14101 cgatgggggg aatgacaaca tgcgccttgt tggctaaaca tcctcatata gtagcgggcg
    14161 tgtgtttgat gggttcccct gcacctttgg aatatggaaa agaaatcgta aggcgcgcat
    14221 cggaatataa ttatgtcgta ccaaaagact attttgattt gatttcatgg gttaagagct
    14281 atgatttgtc attgcagccg gaaaagttag ctggacgtcc tctgttcttt tggcatggac
    14341 aagctgacga aaaaatccct tatatacaaa cggaacgatt tgtagaaaaa aatcggcata
    14401 agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac acagcgacat atggtacaaa
    14461 taccactaat ggaagaagct tccacttttt tagcaaaaaa tttgataaaa aagtataaat
    14521 aattgaaacg gaaaatcact catgacgata gaggattttc cgttttttat tttgggctat
    14581 aataaactct gttaggaaaa tttggtataa ttgatagtaa gaatgacaac atgttagaaa
    14641 ggaagagagc atgcccactt tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac
    14701 ggtctctcga gtcatcaatc atccggaaca ggtgactgac gaattgaaag agttagtttt
    14761 ctcagcaatg gctgaactgg attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa
    14821 tcggtcgcag atcatcaaat tatttatact ggaagaaatc gacacaactg agccctatta
    14881 catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt
    14941 agttacaaat gatgccttcg acgtcggttc ttgtgatgga tacatcatca caggtgtcag
    15001 aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa
    15061 ttctgaagga tatgattatg tagacagcga taatgaatat ggtacagcaa aagcaacgca
    15121 atatgcctta gaaagagatt atcaaacgat tatttatatc ggtatcgaag tggacgagcc
    15181 ttttgaaatt tcaagggaaa ctggttattt atctgtgatg aaggaagttg atcttcctag
    15241 taaaatcttg cggtttccaa atcattcgac agaagtagaa agatatatga cagaaaactg
    15301 ggcagatttt acagataaaa cttgttttgt atgtagttca gatcggctag cactaggagc
    15361 agtaagaggg attataaatg ctggaggaca cttgcccgaa gaatttggtg tgattggatt
    15421 tgatggggta tttttagacc aaatcagttc acctaaattg acaacgatca agcaagatat
    15481 tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc
    15541 gagtcaaggt tatcgtcgtt tttttcctga actagttatc agagagacaa cgaattaagt
    15601 cttattacta tatatatatt tatgaaattc atcgctattt tcttaaaata atcagcgcat
    15661 tgctactgaa aacaatgaat ctgtgctatt attgagaaaa gttccatttt tgagaaggat
    15721 ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt
    15781 aggcgtaaaa ttaaccaacg acaagaagcg agtattattt gacggacaag aagatctttt
    15841 gactgaacag aatcagtccc cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa
    15901 caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa
    15961 ggaaaatttg gaaaagcata aaggaaaatt accggattat accggccatt accaatcaga
    16021 aatcacatcg acaggacgaa aaaaactttt tggtgattcc tctcgttctg tcagacaaag
    16081 tgaaacaagg acagagtcca agcatactac acgaaacgca tctgttgaca cgaaggtgcg
    16141 ggagcgttct tattttgttc ctaaatatgt acccgcatcg attatcccag accctagaga
    16201 aagcaaaatt tctgaggatc atttaatcga atcgatgaaa aaaacggatg actcttatct
    16261 gttgttcgat acagaaccga cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg
    16321 aacattcaat cgaacaaaaa cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa
    16381 aaaaagccat ggtatcttag aacgctcatt acaaggattg atcgatgatc aaggaaactc
    16441 tttgggagaa aatagctatt ttaaataagt gatatacaaa tttatattcg gctacagttg
    16501 gaaaggaaac gctgcggcgt ttccttattt tatactctat ccgaaagttt atatcgtatc
    16561 ctcaaatttt tttgagttta agcgaatttt ttctcccaat aagtaagaaa ataatggcaa
    16621 aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa tagtttattt ttttataaag
    16681 aagattttac tgaataatat cattttagtt tacataatga tatttctgta aattaaataa
    16741 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
    16801 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
    16861 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
    16921 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
    16981 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
    17041 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
    17101 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
    17161 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
    17221 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
    17281 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
    17341 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
    17401 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
    17461 acttctttat tgtactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
    17521 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
    17581 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
    17641 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
    17701 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
    17761 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
    17821 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
    17881 caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt
    17941 tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt
    18001 ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg
    18061 tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg
    18121 ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt
    18181 gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa
    18241 agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag
    18301 gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg
    18361 cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag
    18421 tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac
    18481 ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt
    18541 tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag
    18601 aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca
    18661 tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg
    18721 ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg
    18781 accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta
    18841 tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt
    18901 tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa
    18961 atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt
    19021 ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca
    19081 agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca
    19141 caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga
    19201 caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat
    19261 tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga
    19321 acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg
    19381 gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg
    19441 gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata
    19501 atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc
    19561 tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga
    19621 aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa
    19681 aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc
    19741 cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt
    19801 gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga
    19861 tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag
    19921 tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag caggagacat
    19981 ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg
    20041 atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt
    20101 catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct
    20161 tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg
    20221 aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt
    20281 gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt
    20341 tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga
    20401 agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga
    20461 cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga
    20521 accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa
    20581 tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata
    20641 ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc
    20701 gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat
    20761 atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag
    20821 aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc
    20881 gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac
    20941 aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat
    21001 tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg
    21061 aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg
    21121 aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac
    21181 cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc
    21241 ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca
    21301 atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg
    21361 aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt
    21421 ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag
    21481 agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat
    21541 ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt
    21601 atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc
    21661 caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg
    21721 caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac
    21781 ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg
    21841 atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga
    21901 cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat
    21961 tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct
    22021 atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat
    22081 gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct
    22141 gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct
    22201 gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg
    22261 tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga
    22321 aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca
    22381 tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag
    22441 cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg
    22501 gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt
    22561 ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt
    22621 tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg
    22681 tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa
    22741 aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat
    22801 ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt
    22861 gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca
    22921 tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca
    22981 ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta
    23041 tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct
    23101 actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa
    23161 ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat
    23221 ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga
    23281 accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt
    23341 aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc
    23401 aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat
    23461 ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga
    23521 agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt
    23581 aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt
    23641 tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt
    23701 gaaagaatta gatgaacggt tagattacta tactccggta attattacga atgatcaact
    23761 gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca
    23821 ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa
    23881 tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc
    23941 tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga
    24001 aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa
    24061 ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg
    24121 aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg
    24181 ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact
    24241 tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat
    24301 atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat
    24361 caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt
    24421 gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga
    24481 acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt
    24541 ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa
    24601 aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca
    24661 gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct
    24721 gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat
    24781 cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca
    24841 gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc
    24901 agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg
    24961 tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa
    25021 agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac
    25081 acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt
    25141 actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca
    25201 ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg
    25261 ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc
    25321 agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt
    25381 aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat
    25441 cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga
    25501 acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt
    25561 tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc
    25621 gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg
    25681 ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa
    25741 ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg
    25801 tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc
    25861 aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg
    25921 tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    25981 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DASHWH010000036        25644 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.036, whole genome shotgun sequence.
ACCESSION   DASHWH010000036 DASHWH010000000
VERSION     DASHWH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.036"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..3658
                     /locus_tag="U2R41_001560"
     CDS             131..3658
                     /locus_tag="U2R41_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEM8888981.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="U2R41_001561"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="U2R41_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEM8888982.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="U2R41_001562"
     CDS             7446..7787
                     /locus_tag="U2R41_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8888983.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="U2R41_001563"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="U2R41_001563"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEM8888984.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="U2R41_001564"
     CDS             9144..11564
                     /locus_tag="U2R41_001564"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEM8888985.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U2R41_001565"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U2R41_001565"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEM8888986.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="U2R41_001566"
     CDS             12998..13735
                     /locus_tag="U2R41_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8888987.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="U2R41_001567"
     CDS             13749..14564
                     /locus_tag="U2R41_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM8888988.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="U2R41_001568"
     CDS             14577..15224
                     /locus_tag="U2R41_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8888989.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="U2R41_001569"
     CDS             15240..15896
                     /locus_tag="U2R41_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8888990.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="U2R41_001570"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="U2R41_001570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEM8888991.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="U2R41_001571"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="U2R41_001571"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEM8888992.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="U2R41_001572"
     CDS             18238..18756
                     /locus_tag="U2R41_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM8888993.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="U2R41_001573"
     CDS             18863..19363
                     /locus_tag="U2R41_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8888994.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="U2R41_001574"
     CDS             complement(19643..20563)
                     /locus_tag="U2R41_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEM8888995.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20728..21741
                     /locus_tag="U2R41_001575"
     CDS             20728..21741
                     /locus_tag="U2R41_001575"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEM8888996.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="U2R41_001576"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="U2R41_001576"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEM8888997.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="U2R41_001577"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="U2R41_001577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM8888998.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="U2R41_001578"
     CDS             complement(23381..24142)
                     /locus_tag="U2R41_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8888999.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="U2R41_001579"
     CDS             24331..25425
                     /locus_tag="U2R41_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8889000.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25511..>25644)
                     /locus_tag="U2R41_001580"
     CDS             complement(25511..>25644)
                     /locus_tag="U2R41_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8889001.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8493 a         4442 c         5408 g         7301 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
    25501 ttatgatttg tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt
    25561 cgattggtgt tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac
    25621 tggaaataaa tgtctgattc actt
//
LOCUS       DASHWH010000037        24573 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.037, whole genome shotgun sequence.
ACCESSION   DASHWH010000037 DASHWH010000000
VERSION     DASHWH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.037"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(153..551)
                     /locus_tag="U2R41_001581"
     CDS             complement(153..551)
                     /locus_tag="U2R41_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEM8889002.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="U2R41_001582"
     CDS             complement(548..1321)
                     /locus_tag="U2R41_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM8889003.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="U2R41_001583"
     CDS             complement(1318..2001)
                     /locus_tag="U2R41_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM8889004.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="U2R41_001584"
     CDS             complement(2168..3055)
                     /locus_tag="U2R41_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8889005.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="U2R41_001585"
     CDS             complement(3052..3921)
                     /locus_tag="U2R41_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8889006.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMFFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="U2R41_001586"
     CDS             complement(4058..5344)
                     /locus_tag="U2R41_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8889007.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="U2R41_001587"
     CDS             5617..6195
                     /locus_tag="U2R41_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8889008.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="U2R41_001588"
     CDS             6195..7124
                     /locus_tag="U2R41_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889009.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="U2R41_001589"
     CDS             7121..8857
                     /locus_tag="U2R41_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM8889010.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="U2R41_001590"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="U2R41_001590"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEM8889011.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="U2R41_001591"
     CDS             complement(9753..10262)
                     /locus_tag="U2R41_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEM8889012.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="U2R41_001592"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="U2R41_001592"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEM8889013.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="U2R41_001593"
     CDS             complement(11563..12654)
                     /locus_tag="U2R41_001593"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEM8889014.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="U2R41_001594"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="U2R41_001594"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEM8889015.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="U2R41_001595"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="U2R41_001595"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEM8889016.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="U2R41_001596"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="U2R41_001596"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEM8889017.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="U2R41_001597"
     CDS             complement(16617..17591)
                     /locus_tag="U2R41_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEM8889018.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="U2R41_001598"
     CDS             complement(17572..19455)
                     /locus_tag="U2R41_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEM8889019.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="U2R41_001599"
     CDS             complement(19473..21698)
                     /locus_tag="U2R41_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEM8889020.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="U2R41_001600"
     CDS             21813..22847
                     /locus_tag="U2R41_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8889021.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="U2R41_001601"
     CDS             complement(22914..23927)
                     /locus_tag="U2R41_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8889022.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="U2R41_001602"
     CDS             complement(24005..24193)
                     /locus_tag="U2R41_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEM8889023.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5340 c         4397 g         7774 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaaaca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taa
//
LOCUS       DASHWH010000038        24270 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.038, whole genome shotgun sequence.
ACCESSION   DASHWH010000038 DASHWH010000000
VERSION     DASHWH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.038"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..572
                     /locus_tag="U2R41_001603"
     CDS             <1..572
                     /locus_tag="U2R41_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8889024.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="U2R41_001604"
     CDS             576..1217
                     /locus_tag="U2R41_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889025.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="U2R41_001605"
     CDS             1214..2131
                     /locus_tag="U2R41_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8889026.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="U2R41_001606"
     CDS             2128..2799
                     /locus_tag="U2R41_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889027.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="U2R41_001607"
     CDS             complement(3340..3828)
                     /locus_tag="U2R41_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEM8889028.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="U2R41_001608"
     CDS             complement(4029..4295)
                     /locus_tag="U2R41_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM8889029.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="U2R41_001609"
     CDS             complement(4295..4558)
                     /locus_tag="U2R41_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8889030.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFFEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="U2R41_001610"
     CDS             5034..5219
                     /locus_tag="U2R41_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8889031.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="U2R41_001611"
     CDS             complement(5361..7184)
                     /locus_tag="U2R41_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEM8889032.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="U2R41_001612"
     CDS             complement(7782..10487)
                     /locus_tag="U2R41_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEM8889033.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="U2R41_001613"
     CDS             complement(10793..11740)
                     /locus_tag="U2R41_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEM8889034.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="U2R41_001614"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="U2R41_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM8889035.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="U2R41_001615"
     CDS             complement(12375..12737)
                     /locus_tag="U2R41_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8889036.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="U2R41_001616"
     CDS             complement(12748..13869)
                     /locus_tag="U2R41_001616"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEM8889037.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="U2R41_001617"
     CDS             complement(13885..14235)
                     /locus_tag="U2R41_001617"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEM8889038.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="U2R41_001618"
     CDS             complement(14365..15876)
                     /locus_tag="U2R41_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8889039.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="U2R41_001619"
     CDS             complement(16198..17550)
                     /locus_tag="U2R41_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEM8889040.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="U2R41_001620"
     CDS             complement(17703..18779)
                     /locus_tag="U2R41_001620"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEM8889041.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="U2R41_001621"
     CDS             18975..20720
                     /locus_tag="U2R41_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEM8889042.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="U2R41_001622"
     CDS             complement(21000..21437)
                     /locus_tag="U2R41_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEM8889043.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="U2R41_001623"
     CDS             complement(21421..22794)
                     /locus_tag="U2R41_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889044.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="U2R41_001624"
     CDS             complement(23116..23604)
                     /locus_tag="U2R41_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889045.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="U2R41_001625"
     CDS             complement(23626..24228)
                     /locus_tag="U2R41_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8889046.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4487 g         7660 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaaa aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc
//
LOCUS       DASHWH010000039        23819 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.039, whole genome shotgun sequence.
ACCESSION   DASHWH010000039 DASHWH010000000
VERSION     DASHWH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.039"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..779)
                     /locus_tag="U2R41_001626"
     CDS             complement(45..779)
                     /locus_tag="U2R41_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM8889047.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="U2R41_001627"
     CDS             1106..1516
                     /locus_tag="U2R41_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEM8889048.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="U2R41_001628"
     CDS             1509..2210
                     /locus_tag="U2R41_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEM8889049.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="U2R41_001629"
     CDS             2210..3796
                     /locus_tag="U2R41_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889050.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="U2R41_001630"
     CDS             3815..4036
                     /locus_tag="U2R41_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEM8889051.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="U2R41_001631"
     CDS             4033..4794
                     /locus_tag="U2R41_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEM8889052.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="U2R41_001632"
     CDS             5174..5683
                     /locus_tag="U2R41_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM8889053.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="U2R41_001633"
     CDS             complement(5758..6297)
                     /locus_tag="U2R41_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEM8889054.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="U2R41_001634"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="U2R41_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEM8889055.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="U2R41_001635"
     CDS             7310..8161
                     /locus_tag="U2R41_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEM8889056.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="U2R41_001636"
     CDS             8189..8824
                     /locus_tag="U2R41_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEM8889057.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="U2R41_001637"
     CDS             8844..9617
                     /locus_tag="U2R41_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8889058.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="U2R41_001638"
     CDS             10025..10822
                     /locus_tag="U2R41_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEM8889059.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="U2R41_001639"
     CDS             10800..11213
                     /locus_tag="U2R41_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8889060.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="U2R41_001640"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="U2R41_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM8889061.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="U2R41_001641"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="U2R41_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEM8889062.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /gene="maf"
                     /locus_tag="U2R41_001642"
     CDS             15990..16550
                     /gene="maf"
                     /locus_tag="U2R41_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEM8889063.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="U2R41_001643"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="U2R41_001643"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEM8889064.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="U2R41_001644"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="U2R41_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEM8889065.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="U2R41_001645"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="U2R41_001645"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEM8889066.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="U2R41_001646"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="U2R41_001646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEM8889067.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="U2R41_001647"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="U2R41_001647"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEM8889068.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="U2R41_001648"
     CDS             21897..22475
                     /locus_tag="U2R41_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8889069.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="U2R41_001649"
     CDS             22527..23009
                     /locus_tag="U2R41_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEM8889070.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="U2R41_001650"
     CDS             23126..23500
                     /locus_tag="U2R41_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889071.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4284 c         5115 g         6646 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagtt
//
LOCUS       DASHWH010000040        23067 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.040, whole genome shotgun sequence.
ACCESSION   DASHWH010000040 DASHWH010000000
VERSION     DASHWH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.040"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            103..273
                     /locus_tag="U2R41_001651"
     CDS             103..273
                     /locus_tag="U2R41_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889072.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            493..1200
                     /locus_tag="U2R41_001652"
     CDS             493..1200
                     /locus_tag="U2R41_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8889073.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="U2R41_001653"
     CDS             complement(1259..1903)
                     /locus_tag="U2R41_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEM8889074.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="U2R41_001654"
     CDS             2030..2317
                     /locus_tag="U2R41_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889075.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="U2R41_001655"
     CDS             2425..2745
                     /locus_tag="U2R41_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889076.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="U2R41_001656"
     CDS             2826..4139
                     /locus_tag="U2R41_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM8889077.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="U2R41_001657"
     CDS             4136..4855
                     /locus_tag="U2R41_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEM8889078.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="U2R41_001658"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="U2R41_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEM8889079.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="U2R41_001659"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="U2R41_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEM8889080.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="U2R41_001660"
     CDS             join(8416..8487,8489..9517)
                     /gene="prfB"
                     /locus_tag="U2R41_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEM8889081.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="U2R41_001661"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="U2R41_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEM8889082.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="U2R41_001662"
     CDS             10292..11185
                     /locus_tag="U2R41_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889083.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="U2R41_001663"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="U2R41_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM8889084.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="U2R41_001664"
     CDS             13122..13979
                     /locus_tag="U2R41_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEM8889085.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="U2R41_001665"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="U2R41_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEM8889086.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="U2R41_001666"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="U2R41_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEM8889087.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="U2R41_001667"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="U2R41_001667"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM8889088.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="U2R41_001668"
     CDS             16735..17493
                     /locus_tag="U2R41_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM8889089.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="U2R41_001669"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="U2R41_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEM8889090.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="U2R41_001670"
     CDS             18406..19437
                     /locus_tag="U2R41_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM8889091.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="U2R41_001671"
     CDS             19434..20201
                     /locus_tag="U2R41_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM8889092.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="U2R41_001672"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="U2R41_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEM8889093.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22045..22353
                     /locus_tag="U2R41_001673"
     CDS             22045..22353
                     /locus_tag="U2R41_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEM8889094.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22353..22703
                     /locus_tag="U2R41_001674"
     CDS             22353..22703
                     /locus_tag="U2R41_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEM8889095.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>23067
                     /locus_tag="U2R41_001675"
     CDS             22841..>23067
                     /locus_tag="U2R41_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889096.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAH"
BASE COUNT         7597 a         3972 c         4964 g         6534 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    22981 aggtaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtgggag
    23041 aaaagtcatt caattacctg ctcatga
//
LOCUS       DASHWH010000041        22857 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.041, whole genome shotgun sequence.
ACCESSION   DASHWH010000041 DASHWH010000000
VERSION     DASHWH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.041"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(104..1336)
                     /locus_tag="U2R41_001676"
     CDS             complement(104..1336)
                     /locus_tag="U2R41_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM8889097.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U2R41_001677"
     CDS             1591..2160
                     /locus_tag="U2R41_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8889098.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U2R41_001678"
     CDS             complement(2338..2778)
                     /locus_tag="U2R41_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEM8889099.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U2R41_001679"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U2R41_001679"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEM8889100.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U2R41_001680"
     CDS             3732..4655
                     /locus_tag="U2R41_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEM8889101.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U2R41_001681"
     CDS             4731..5870
                     /locus_tag="U2R41_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEM8889102.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U2R41_001682"
     CDS             5863..6663
                     /locus_tag="U2R41_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8889103.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U2R41_001683"
     CDS             6663..7490
                     /locus_tag="U2R41_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8889104.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U2R41_001684"
     CDS             7468..8202
                     /locus_tag="U2R41_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8889105.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U2R41_001685"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U2R41_001685"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEM8889106.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U2R41_001686"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U2R41_001686"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEM8889107.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U2R41_001687"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U2R41_001687"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEM8889108.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U2R41_001688"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U2R41_001688"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEM8889109.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U2R41_001689"
     CDS             11787..13820
                     /locus_tag="U2R41_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889110.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="U2R41_001690"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="U2R41_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="U2R41_001691"
     CDS             15353..16159
                     /locus_tag="U2R41_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889111.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="U2R41_001692"
     CDS             16171..17391
                     /locus_tag="U2R41_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889112.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="U2R41_001693"
     CDS             17381..18967
                     /locus_tag="U2R41_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8889113.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="U2R41_001694"
     CDS             19006..21144
                     /locus_tag="U2R41_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8889114.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="U2R41_001695"
     CDS             complement(21512..22495)
                     /locus_tag="U2R41_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEM8889115.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4711 g         7071 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagt
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DASHWH010000042        22322 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.043, whole genome shotgun sequence.
ACCESSION   DASHWH010000042 DASHWH010000000
VERSION     DASHWH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.043"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            2..400
                     /locus_tag="U2R41_001696"
     CDS             2..400
                     /locus_tag="U2R41_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM8889116.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="U2R41_001697"
     CDS             384..1079
                     /locus_tag="U2R41_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEM8889117.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="U2R41_001698"
     CDS             complement(1673..2560)
                     /locus_tag="U2R41_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM8889118.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="U2R41_001699"
     CDS             2745..3584
                     /locus_tag="U2R41_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8889119.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="U2R41_001700"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="U2R41_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEM8889120.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="U2R41_001701"
     CDS             5052..5408
                     /locus_tag="U2R41_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889121.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="U2R41_001702"
     CDS             5411..6568
                     /locus_tag="U2R41_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889122.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="U2R41_001703"
     CDS             complement(6594..7034)
                     /locus_tag="U2R41_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM8889123.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="U2R41_001704"
     CDS             7194..7700
                     /locus_tag="U2R41_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8889124.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9780
                     /locus_tag="U2R41_001705"
                     /pseudo
     CDS             7868..9780
                     /locus_tag="U2R41_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            9785..10786
                     /locus_tag="U2R41_001706"
     CDS             9785..10786
                     /locus_tag="U2R41_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8889125.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10988..11194)
                     /locus_tag="U2R41_001707"
     CDS             complement(10988..11194)
                     /locus_tag="U2R41_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEM8889126.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11840..12688)
                     /locus_tag="U2R41_001708"
     CDS             complement(11840..12688)
                     /locus_tag="U2R41_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8889127.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12687..13754
                     /gene="gnd"
                     /locus_tag="U2R41_001709"
     CDS             12687..13754
                     /gene="gnd"
                     /locus_tag="U2R41_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEM8889128.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13756..14757
                     /locus_tag="U2R41_001710"
     CDS             13756..14757
                     /locus_tag="U2R41_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEM8889129.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14754..15038
                     /locus_tag="U2R41_001711"
     CDS             14754..15038
                     /locus_tag="U2R41_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEM8889130.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15071..15868
                     /locus_tag="U2R41_001712"
     CDS             15071..15868
                     /locus_tag="U2R41_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8889131.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15882..16715
                     /locus_tag="U2R41_001713"
     CDS             15882..16715
                     /locus_tag="U2R41_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889132.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16733..17590
                     /locus_tag="U2R41_001714"
     CDS             16733..17590
                     /locus_tag="U2R41_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8889133.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17612..18121
                     /locus_tag="U2R41_001715"
     CDS             17612..18121
                     /locus_tag="U2R41_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889134.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18158..18559
                     /locus_tag="U2R41_001716"
     CDS             18158..18559
                     /locus_tag="U2R41_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889135.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18671..19024
                     /locus_tag="U2R41_001717"
     CDS             18671..19024
                     /locus_tag="U2R41_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889136.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19058..19501
                     /locus_tag="U2R41_001718"
     CDS             19058..19501
                     /locus_tag="U2R41_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM8889137.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19518..20603
                     /gene="uxuA"
                     /locus_tag="U2R41_001719"
     CDS             19518..20603
                     /gene="uxuA"
                     /locus_tag="U2R41_001719"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM8889138.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20596..21309
                     /locus_tag="U2R41_001720"
     CDS             20596..21309
                     /locus_tag="U2R41_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8889139.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21516..21665)
                     /locus_tag="U2R41_001721"
     CDS             complement(21516..21665)
                     /locus_tag="U2R41_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889140.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22061..22261
                     /locus_tag="U2R41_001722"
     CDS             22061..22261
                     /locus_tag="U2R41_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8889141.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7686 a         3430 c         4624 g         6582 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct catttattca
     9721 ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa gagagggtaa
     9781 atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg tagttatttt
     9841 agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc ttgttagaaa
     9901 acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt tccagcaaaa
     9961 aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca aaaataataa
    10021 tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt tctggatgca
    10081 tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg aatatctatg
    10141 ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg aagcaaaaca
    10201 tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag agataaaaga
    10261 tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac aaattagtgg
    10321 agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc ttgatccgac
    10381 tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg atccttctgc
    10441 acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg gatgggctta
    10501 ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct tggtcagaca
    10561 tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata cgtttacgaa
    10621 acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca caaaagtaga
    10681 ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca gcaataaaca
    10741 agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc ttatgatgca
    10801 aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca ataaacaagc
    10861 ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg caaggggcac
    10921 cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt tacgtaaatt
    10981 ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg aagcctgcgg
    11041 aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc actaatattc
    11101 cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc aatgcactag
    11161 cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt tttataattg
    11221 tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt gaataaaatg
    11281 atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga ttcagcaatc
    11341 agataggtac aaagtttacc atcatacgtc tagaccaata atagaaaata tgggaatata
    11401 atagaaaaga tttaggatac ccccaaaaga atttccccaa aaattcgatg tatcctaaac
    11461 ttttttattt tacataacaa tcatatcaac tgtgaaataa aattacaaat gataacctaa
    11521 aatgaggcca aaagccagtg ttatcaagga aatacttaat tcaccaaaat tttttttaat
    11581 tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt tttactactg
    11641 tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc
    11701 caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc
    11761 agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt
    11821 cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga
    11881 tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa
    11941 tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa
    12001 tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa
    12061 cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt
    12121 gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca
    12181 attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat
    12241 aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt
    12301 cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat
    12361 ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt
    12421 tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca
    12481 ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt
    12541 cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact ttgcttggat
    12601 tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt
    12661 tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat aagattatca
    12721 ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa
    12781 atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg
    12841 gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt
    12901 taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta
    12961 gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt
    13021 ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt
    13081 taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt
    13141 attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg
    13201 attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag
    13261 gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag
    13321 gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa
    13381 tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt
    13441 atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct
    13501 ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg
    13561 gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg
    13621 ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata
    13681 gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg
    13741 aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga
    13801 agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact
    13861 gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt
    13921 attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta
    13981 ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac
    14041 cagacaatcg tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat
    14101 ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac
    14161 gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt
    14221 gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc
    14281 tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca
    14341 aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat
    14401 ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat
    14461 gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt
    14521 ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt
    14581 caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac
    14641 acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt
    14701 cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga
    14761 agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag
    14821 gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca
    14881 cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa
    14941 cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa
    15001 tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact
    15061 gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac
    15121 tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca
    15181 agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca
    15241 attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa
    15301 atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa
    15361 gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc
    15421 aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat
    15481 ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa atgcggcagc
    15541 acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg
    15601 agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat
    15661 agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg
    15721 attatttccg aaagataaac agataataga taaaatggct gaatttattg attcgaaaga
    15781 gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga
    15841 actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag
    15901 tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag
    15961 gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca
    16021 tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg
    16081 ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac
    16141 cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg
    16201 gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat
    16261 atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt
    16321 atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag
    16381 gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc
    16441 ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg
    16501 gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat
    16561 ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg
    16621 tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt
    16681 ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga
    16741 aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc
    16801 ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc
    16861 atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt
    16921 aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg
    16981 tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga
    17041 aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc
    17101 actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat
    17161 cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt
    17221 tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg
    17281 taatgctaca ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt
    17341 tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt
    17401 gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc
    17461 agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt
    17521 gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg
    17581 tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga
    17641 tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc
    17701 catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa
    17761 tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta
    17821 ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac
    17881 gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc
    17941 tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga
    18001 tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc
    18061 cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata
    18121 aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg
    18181 tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca
    18241 gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat
    18301 tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc
    18361 agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat
    18421 gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt
    18481 gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt
    18541 cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt
    18601 attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc
    18661 catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt
    18721 tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa
    18781 agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg
    18841 aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa
    18901 cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac
    18961 agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg
    19021 ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac
    19081 acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag
    19141 ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt
    19201 caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat
    19261 tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa
    19321 agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca
    19381 tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga
    19441 taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta
    19501 gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga
    19561 tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga
    19621 ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg
    19681 agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg
    19741 cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt
    19801 ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa
    19861 ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg
    19921 tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca
    19981 aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg
    20041 aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc
    20101 cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac
    20161 tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag
    20221 tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa
    20281 acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa
    20341 acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct
    20401 cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac
    20461 gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg
    20521 atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac
    20581 cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta
    20641 ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat
    20701 gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa
    20761 caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat
    20821 caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa
    20881 gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca
    20941 cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc
    21001 atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt
    21061 tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc
    21121 gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg
    21181 cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa
    21241 gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg
    21301 tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag
    21361 ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac
    21421 aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga
    21481 agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt taattcttgc
    21541 actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt gtcaaaaacg
    21601 acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt gttatagaat
    21661 gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc gttgataacg
    21721 aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga aattttaaag
    21781 tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca atcgcggtta
    21841 ttcaactttt tcttcgattc gaagacaata gaagacaatg aattttatat ttcattgcat
    21901 tatccttgtg ttattagaaa aagcatgata tattaaatga gtaatctatt ttgaaacgta
    21961 atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac
    22021 gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg gtacagtaaa
    22081 atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa atgatgtatt
    22141 tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag gccaagcagt
    22201 gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg ttaaagctta
    22261 ataaaaattg acttttaacc gtcttttcga aagaaaagac ggtttttttg tacgaaaaaa
    22321 tt
//
LOCUS       DASHWH010000043        22237 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.044, whole genome shotgun sequence.
ACCESSION   DASHWH010000043 DASHWH010000000
VERSION     DASHWH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.044"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            170..514
                     /locus_tag="U2R41_001723"
     CDS             170..514
                     /locus_tag="U2R41_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEM8889142.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="U2R41_001724"
                     /pseudo
     CDS             515..934
                     /locus_tag="U2R41_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="U2R41_001725"
     CDS             complement(1026..1376)
                     /locus_tag="U2R41_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889143.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="U2R41_001726"
     CDS             complement(1342..2673)
                     /locus_tag="U2R41_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEM8889144.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="U2R41_001727"
     CDS             2929..3495
                     /locus_tag="U2R41_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8889145.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="U2R41_001728"
     tRNA            3640..3727
                     /locus_tag="U2R41_001728"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="U2R41_001729"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="U2R41_001729"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEM8889146.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="U2R41_001730"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="U2R41_001730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEM8889147.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="U2R41_001731"
     CDS             complement(7450..7896)
                     /locus_tag="U2R41_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM8889148.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="U2R41_001732"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="U2R41_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8889149.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="U2R41_001733"
     CDS             8361..8879
                     /locus_tag="U2R41_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8889150.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="U2R41_001734"
     CDS             complement(8956..9912)
                     /locus_tag="U2R41_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8889151.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="U2R41_001735"
     CDS             10078..10884
                     /locus_tag="U2R41_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889152.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="U2R41_001736"
     CDS             10881..11870
                     /locus_tag="U2R41_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM8889153.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="U2R41_001737"
     CDS             11867..12871
                     /locus_tag="U2R41_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM8889154.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="U2R41_001738"
     CDS             13003..13545
                     /locus_tag="U2R41_001738"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEM8889155.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="U2R41_001739"
     CDS             13565..14263
                     /locus_tag="U2R41_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEM8889156.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="U2R41_001740"
     CDS             14284..14496
                     /locus_tag="U2R41_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEM8889157.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="U2R41_001741"
     CDS             14496..15458
                     /locus_tag="U2R41_001741"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEM8889158.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="U2R41_001742"
     CDS             15476..15871
                     /locus_tag="U2R41_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM8889159.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="U2R41_001743"
     CDS             16034..16399
                     /locus_tag="U2R41_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEM8889160.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="U2R41_001744"
     CDS             16396..18207
                     /locus_tag="U2R41_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEM8889161.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="U2R41_001745"
     CDS             complement(18269..18436)
                     /locus_tag="U2R41_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEM8889162.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="U2R41_001746"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="U2R41_001746"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEM8889163.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="U2R41_001747"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="U2R41_001747"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEM8889164.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="U2R41_001748"
     CDS             21193..22173
                     /locus_tag="U2R41_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8889165.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7212 a         3806 c         4628 g         6591 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaaaaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttgttcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcagcc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatatc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat cttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaatgaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggtttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct ctctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg gggaaatgaa
    22201 gtgataccgc agcttttttg tgtttcagga aggggca
//
LOCUS       DASHWH010000044        22114 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.045, whole genome shotgun sequence.
ACCESSION   DASHWH010000044 DASHWH010000000
VERSION     DASHWH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.045"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..608)
                     /locus_tag="U2R41_001749"
     CDS             complement(21..608)
                     /locus_tag="U2R41_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889166.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            783..2105
                     /locus_tag="U2R41_001750"
     CDS             783..2105
                     /locus_tag="U2R41_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8889167.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2260..3036
                     /locus_tag="U2R41_001751"
     CDS             2260..3036
                     /locus_tag="U2R41_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM8889168.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3029..3598
                     /gene="rnmV"
                     /locus_tag="U2R41_001752"
     CDS             3029..3598
                     /gene="rnmV"
                     /locus_tag="U2R41_001752"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEM8889169.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3591..4475
                     /gene="rsmA"
                     /locus_tag="U2R41_001753"
     CDS             3591..4475
                     /gene="rsmA"
                     /locus_tag="U2R41_001753"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEM8889170.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4926..5555
                     /locus_tag="U2R41_001754"
     CDS             4926..5555
                     /locus_tag="U2R41_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889171.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5679..6788)
                     /gene="ugpC"
                     /locus_tag="U2R41_001755"
     CDS             complement(5679..6788)
                     /gene="ugpC"
                     /locus_tag="U2R41_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEM8889172.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            6997..7419
                     /gene="mgsA"
                     /locus_tag="U2R41_001756"
     CDS             6997..7419
                     /gene="mgsA"
                     /locus_tag="U2R41_001756"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEM8889173.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7742..8278
                     /locus_tag="U2R41_001757"
     CDS             7742..8278
                     /locus_tag="U2R41_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM8889174.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8318..10081)
                     /locus_tag="U2R41_001758"
     CDS             complement(8318..10081)
                     /locus_tag="U2R41_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889175.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10084..11799)
                     /locus_tag="U2R41_001759"
     CDS             complement(10084..11799)
                     /locus_tag="U2R41_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889176.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(12578..12802)
                     /locus_tag="U2R41_001760"
     CDS             complement(12578..12802)
                     /locus_tag="U2R41_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889177.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            12993..15770
                     /locus_tag="U2R41_001761"
     CDS             12993..15770
                     /locus_tag="U2R41_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8889178.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            15851..16270
                     /locus_tag="U2R41_001762"
     CDS             15851..16270
                     /locus_tag="U2R41_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEM8889179.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16282..16752
                     /locus_tag="U2R41_001763"
     CDS             16282..16752
                     /locus_tag="U2R41_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889180.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            16769..17548
                     /locus_tag="U2R41_001764"
     CDS             16769..17548
                     /locus_tag="U2R41_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889181.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17535..18356
                     /locus_tag="U2R41_001765"
     CDS             17535..18356
                     /locus_tag="U2R41_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8889182.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18373..19422
                     /locus_tag="U2R41_001766"
     CDS             18373..19422
                     /locus_tag="U2R41_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889183.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19435..20439
                     /locus_tag="U2R41_001767"
     CDS             19435..20439
                     /locus_tag="U2R41_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889184.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21072..22091
                     /locus_tag="U2R41_001768"
     CDS             21072..22091
                     /locus_tag="U2R41_001768"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM8889185.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         7144 a         3880 c         4550 g         6540 t
ORIGIN      
        1 tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa
       61 cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc
      121 gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac
      181 cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt
      241 gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc
      301 tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc
      361 ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg
      421 atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca
      481 acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct
      541 ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta
      601 aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa
      661 agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa
      721 attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg
      781 gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg
      841 gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt
      901 ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat
      961 tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg
     1021 aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact
     1081 ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac
     1141 aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt
     1201 tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat
     1261 taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc
     1321 agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt
     1381 tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa
     1441 tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag
     1501 tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg
     1561 gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc
     1621 tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg
     1681 ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga
     1741 caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag
     1801 cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga
     1861 tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg
     1921 aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg
     1981 gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta
     2041 taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag
     2101 agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc
     2161 tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt
     2221 gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc
     2281 catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa
     2341 atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg
     2401 caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct
     2461 ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt
     2521 gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa
     2581 gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt
     2641 catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat
     2701 atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac
     2761 ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa
     2821 gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg
     2881 gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa
     2941 aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat
     3001 gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt
     3061 cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga
     3121 aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac
     3181 tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat
     3241 tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa
     3301 gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa
     3361 aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct
     3421 tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga
     3481 cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg
     3541 tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat
     3601 atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat
     3661 ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag
     3721 aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat
     3781 tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt
     3841 tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag
     3901 atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt
     3961 taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg
     4021 aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta
     4081 tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata
     4141 tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag
     4201 attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa
     4261 aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata
     4321 acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg
     4381 aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg
     4441 cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt
     4501 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt
     4561 ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg
     4621 ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat
     4681 gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac
     4741 aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc
     4801 ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag
     4861 ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag
     4921 aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg
     4981 gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg
     5041 cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg
     5101 cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat
     5161 tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg
     5221 ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga
     5281 tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta
     5341 atatgcctga aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga
     5401 ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac
     5461 tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt
     5521 cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc
     5581 tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg
     5641 caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt
     5701 tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg
     5761 cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt
     5821 gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg
     5881 gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg
     5941 aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga
     6001 atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag
     6061 aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa
     6121 acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca
     6181 gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc
     6241 tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat
     6301 aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca
     6361 cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct
     6421 aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat
     6481 ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg
     6541 aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata
     6601 tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt
     6661 ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa
     6721 tcagttacag aataattttc tgcattgtca tattttttat atacgttttt cagagccatt
     6781 tctaccataa tacgttccac actttctttt ttagttttat taactattta aagtataaat
     6841 gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat
     6901 gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa
     6961 ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat
     7021 gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa
     7081 catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg
     7141 atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg
     7201 gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa
     7261 ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat
     7321 atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga
     7381 atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat
     7441 aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata
     7501 taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat
     7561 gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat
     7621 aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc
     7681 ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa
     7741 aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt
     7801 gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac
     7861 atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc
     7921 agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt
     7981 tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat
     8041 cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt
     8101 gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc
     8161 gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt
     8221 aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa
     8281 aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt
     8341 gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga
     8401 caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat
     8461 gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt
     8521 tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac
     8581 tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt
     8641 gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag
     8701 aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat
     8761 tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac
     8821 gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg
     8881 ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc
     8941 ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct
     9001 gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg
     9061 gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa
     9121 atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga
     9181 taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga
     9241 ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa
     9301 ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc
     9361 ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct
     9421 gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc
     9481 caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca
     9541 caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca
     9601 tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg
     9661 cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt
     9721 gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc
     9781 ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg agccattgac
     9841 tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga
     9901 agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca
     9961 caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat
    10021 aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca
    10081 taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa
    10141 ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc
    10201 ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg
    10261 tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa
    10321 aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc
    10381 gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg
    10441 gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc
    10501 tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg
    10561 aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc
    10621 cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt
    10681 tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt
    10741 aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc
    10801 gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt
    10861 ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac
    10921 gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc
    10981 aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa
    11041 acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt
    11101 ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc
    11161 tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg
    11221 ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat
    11281 gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc
    11341 ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa
    11401 taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt
    11461 gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt
    11521 gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg
    11581 gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc
    11641 aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa
    11701 aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg
    11761 cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat
    11821 ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa
    11881 agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata
    11941 gactattttt tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca ttcataggaa
    12001 cgtctatttt acttaatttt tctataaatg aattctgcat aaaaaatata tcaagtaaat
    12061 gtttatagat gaaactctat ttacttcttt tttaatttgt tcttgcaatc ttttttgcta
    12121 cgtaattttt gtaaaaaagt gggttgagag aaatctaatt gattttcaaa gaaggagtat
    12181 gaaaatgaga aaaatattta ttcgtttatt gtgggtacta tcttagagga ataaataata
    12241 gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc
    12301 ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg
    12361 taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg
    12421 tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata
    12481 actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat
    12541 agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat
    12601 agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat
    12661 ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa
    12721 ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt
    12781 ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa
    12841 attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata
    12901 agtaggctgc tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac
    12961 aaaatgaatc cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt
    13021 tatgttactt agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta
    13081 cagcacaaag gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga
    13141 accagttaaa tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc
    13201 acaaagaagc ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg
    13261 atgatctcaa agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga
    13321 tcggatacga cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc
    13381 cagatggcgg attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag
    13441 ttaatttggt ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca
    13501 tcacttttaa ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg
    13561 gacatgttaa gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag
    13621 cagatggtgg gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa
    13681 aactgttcac ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac
    13741 gccggatcaa aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa
    13801 caactttcat tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc
    13861 gtaatgaacg gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga
    13921 gccctttgtc agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa
    13981 acataggaga gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc
    14041 gtgaacagcc tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc
    14101 ctattactaa aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc
    14161 aattaatgga gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc
    14221 taattgattt caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag
    14281 aagtgattca attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc
    14341 tgctggttta ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc
    14401 aaatcaaatt cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca
    14461 ctatcaaatg gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac
    14521 aagtttctca gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta
    14581 agatcaaatt agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa
    14641 aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt
    14701 atgcaactgc tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg
    14761 aatcatttga tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt
    14821 attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga
    14881 aagaaatcca tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg
    14941 tgacgacacc tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag
    15001 agatagcaga agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg
    15061 aaaacaaacc caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact
    15121 taagtgaact gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg
    15181 aatatcagca attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg
    15241 taggaatgat tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag
    15301 acctcatctc gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg
    15361 cttcagagaa ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac
    15421 tggattccgt gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga
    15481 gagaattaga ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg
    15541 tccatgtgag ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag
    15601 gattagagaa atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta
    15661 gtgtcattga agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg
    15721 atattttgag tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa
    15781 gttgttgcca ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg
    15841 agatgaaaaa gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat
    15901 attagattca ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta
    15961 tactgatgag aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga
    16021 taaagaaatc attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca
    16081 atatatcaat cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga
    16141 gctagtatct cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac
    16201 tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga
    16261 cgaattttag gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac
    16321 ggacaagtag ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat
    16381 gatgatgtac ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga
    16441 gtcaaactag tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca
    16501 gataaatata agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa
    16561 gcgtatccag aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc
    16621 agcatcggaa aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt
    16681 gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga
    16741 gaagttttgt aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt
    16801 tgcatttgtc gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt
    16861 gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc
    16921 aactttagag ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt
    16981 tgtgaccggt gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac
    17041 cgcgctttta ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt
    17101 agggcttttg attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta
    17161 taaaggagtc gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt
    17221 tgtggtaatg gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat
    17281 tcctgacttc gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt
    17341 tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt
    17401 tgctatagct tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt
    17461 agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg
    17521 ggaggtagaa gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc
    17581 taaatcacgt attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga
    17641 tgaatatggc ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag
    17701 aagaaatggc agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg
    17761 ttacactgat tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat
    17821 ttgatgtgac tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag
    17881 gagattcttt cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag
    17941 ccatgcaagg aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca
    18001 ttctgtttcg ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa
    18061 agatccaggc aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga
    18121 tggtcgttgg agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt
    18181 caggagaaaa tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat
    18241 gtcttggagc atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga
    18301 tcttgatcgg actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa
    18361 ggagcagaga aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt
    18421 acgatcttga aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta
    18481 ctgatcttag caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa
    18541 tcttatggtg atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa
    18601 ctttctcctt caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact
    18661 gttgaagcag taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac
    18721 gtgcaaagtc cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa
    18781 tcagtaggct tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg
    18841 ataggaattg gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa
    18901 cagttgcgca aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt
    18961 gatggatatc aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca
    19021 aactggggaa cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca
    19081 caaggatttg agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat
    19141 ttgctattct ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga
    19201 tatatgagtc ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag
    19261 aaaacacttg gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt
    19321 ccgtttcaac tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag
    19381 atttttgaag actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg
    19441 aaattcaatg aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa
    19501 gtagaagaaa tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga
    19561 atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac
    19621 ttgcctgtct ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca
    19681 aaagattcta tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca
    19741 gtagaacaaa tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt
    19801 aagttgtctc agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc
    19861 aaatttttta tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca
    19921 gaatactatg aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa
    19981 gcagatgcat ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt
    20041 attggagcag ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa
    20101 caaagctggc ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag
    20161 atcgtagaag aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta
    20221 tctgaacgtg tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca
    20281 aaagactatc cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta
    20341 ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca
    20401 ttagagatac gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat
    20461 ataaaaaata aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca
    20521 attacgttat aaaacacatt ttttgcatta tctacagttc aacttataat ctgtttaatg
    20581 ggacatgtgg gtgccaacca ttttttgtta tgcaatagta ctatttagtt gttgcatgga
    20641 tccgtagtaa aagtccaagt acaacgagtt gcgccagttt acatagcgtt ttgtatccag
    20701 aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc
    20761 aggacctgac agatttagaa atgttcaatc tgtctcttat gtattttaaa tcaatactca
    20821 ctaggagaag tttcctaacc tcctaatagt aatgctggta gtaacattgt caaaactgct
    20881 tcctcttagg aacaagcaaa gcataacgag tgtaaaaata aaatgtagac tattgtaaca
    20941 tatcattgat tatttcaagt aaaaacgata aaagaagtaa acaaacactt aaaaacaatg
    21001 gaaattagtc ccaactttaa gaaatgtata ctataatgga aattatcaat tatttatttg
    21061 gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt
    21121 tagcacaaac attggcagaa aacgggcatg atgtccgtat ttggggaaat gtacctgaac
    21181 agattgatga aatcaataag tatcatacaa atcagcattt tttacctgat ttgacgatcc
    21241 cagaatcgat tatcggatat aaagaattgg cagaagcagt ggataacgca gatgctattt
    21301 tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga
    21361 atactaagcc aatcattata catgcaagta aaggactaga acaagatacg cataaacgaa
    21421 tctcagaagt tttggctgaa gaaattccag aagaaaaaag acaggcaatt gttgtattat
    21481 ctggtcctag ccatgctgag gaagtggcag tacatgatat cacaacaatt acagctgcaa
    21541 gtattgatca aaaagcagca gcctatgtac aaaaactgtt catgaatgat tatttcagga
    21601 tttatacaaa taatgatgtg attggcgtag aaactggtgc agcactgaaa aatatcattg
    21661 caattggtgc cggcgctatc catggattag gattcggaga tgatgcaaaa gcagcaatca
    21721 tgactcgagg attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga
    21781 catttatcgg tttgagtggt gtgggggact tgatcgttac ttgtacaagt gttcattctc
    21841 gcaattggcg tgccggtaac ttgttaggac agggacataa gctggaagaa gttctagaaa
    21901 acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac
    21961 agctaggtgt tgagatgcca attactcaaa caatctataa tgttttgtat aatggagaag
    22021 atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt
    22081 ctcttcgtta aatagatgat caatagttaa tcgt
//
LOCUS       DASHWH010000045        21365 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.046, whole genome shotgun sequence.
ACCESSION   DASHWH010000045 DASHWH010000000
VERSION     DASHWH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.046"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            9..851
                     /locus_tag="U2R41_001769"
     CDS             9..851
                     /locus_tag="U2R41_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8889186.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            851..1798
                     /locus_tag="U2R41_001770"
     CDS             851..1798
                     /locus_tag="U2R41_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM8889187.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="U2R41_001771"
     CDS             1813..2046
                     /locus_tag="U2R41_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEM8889188.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="U2R41_001772"
     CDS             2377..2961
                     /locus_tag="U2R41_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8889189.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="U2R41_001773"
     CDS             3092..3760
                     /locus_tag="U2R41_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM8889190.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3771..5186
                     /locus_tag="U2R41_001774"
     CDS             3771..5186
                     /locus_tag="U2R41_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8889191.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="U2R41_001775"
     CDS             5204..5773
                     /locus_tag="U2R41_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEM8889192.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="U2R41_001776"
     CDS             5883..7637
                     /locus_tag="U2R41_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889193.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7597..9363
                     /locus_tag="U2R41_001777"
     CDS             7597..9363
                     /locus_tag="U2R41_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889194.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="U2R41_001778"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="U2R41_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM8889195.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="U2R41_001779"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="U2R41_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM8889196.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="U2R41_001780"
     CDS             10381..10758
                     /locus_tag="U2R41_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889197.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="U2R41_001781"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="U2R41_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8889198.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="U2R41_001782"
     CDS             10952..11935
                     /locus_tag="U2R41_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8889199.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="U2R41_001783"
     CDS             11965..12087
                     /locus_tag="U2R41_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM8889200.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="U2R41_001784"
     CDS             12105..13628
                     /locus_tag="U2R41_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM8889201.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="U2R41_001785"
     CDS             13652..13891
                     /locus_tag="U2R41_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889202.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="U2R41_001786"
     CDS             complement(14084..14545)
                     /locus_tag="U2R41_001786"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8889203.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="U2R41_001787"
     CDS             complement(14740..14958)
                     /locus_tag="U2R41_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889204.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="U2R41_001788"
     CDS             15076..16692
                     /locus_tag="U2R41_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM8889205.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="U2R41_001789"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="U2R41_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="U2R41_001790"
     CDS             complement(18549..18704)
                     /locus_tag="U2R41_001790"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEM8889206.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(19000..19332)
                     /locus_tag="U2R41_001791"
     CDS             complement(19000..19332)
                     /locus_tag="U2R41_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8889207.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="U2R41_001792"
     CDS             complement(19846..20037)
                     /locus_tag="U2R41_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889208.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="U2R41_001793"
     CDS             complement(20131..20517)
                     /locus_tag="U2R41_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8889209.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..>21365
                     /locus_tag="U2R41_001794"
     CDS             21211..>21365
                     /locus_tag="U2R41_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEM8889210.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTD"
BASE COUNT         7264 a         3088 c         4004 g         7009 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataa
//
LOCUS       DASHWH010000046        20747 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.049, whole genome shotgun sequence.
ACCESSION   DASHWH010000046 DASHWH010000000
VERSION     DASHWH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.049"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            168..362
                     /locus_tag="U2R41_001795"
     CDS             168..362
                     /locus_tag="U2R41_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889211.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="U2R41_001796"
     CDS             631..930
                     /locus_tag="U2R41_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889212.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="U2R41_001797"
     CDS             complement(1027..1461)
                     /locus_tag="U2R41_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889213.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="U2R41_001798"
     CDS             complement(1693..2403)
                     /locus_tag="U2R41_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM8889214.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="U2R41_001799"
     CDS             complement(2393..3253)
                     /locus_tag="U2R41_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEM8889215.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="U2R41_001800"
     CDS             complement(3258..3896)
                     /locus_tag="U2R41_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEM8889216.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="U2R41_001801"
     CDS             complement(3911..4210)
                     /locus_tag="U2R41_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889217.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="U2R41_001802"
     CDS             complement(4253..5737)
                     /locus_tag="U2R41_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM8889218.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="U2R41_001803"
     CDS             complement(5757..6218)
                     /locus_tag="U2R41_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8889219.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U2R41_001804"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="U2R41_001804"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEM8889220.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="U2R41_001805"
     CDS             7521..8291
                     /locus_tag="U2R41_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8889221.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="U2R41_001806"
     CDS             complement(8564..9391)
                     /locus_tag="U2R41_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889222.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="U2R41_001807"
     CDS             9959..11194
                     /locus_tag="U2R41_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM8889223.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="U2R41_001808"
     CDS             complement(12193..12453)
                     /locus_tag="U2R41_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM8889224.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="U2R41_001809"
     CDS             complement(12480..13781)
                     /locus_tag="U2R41_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889225.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="U2R41_001810"
     CDS             complement(14073..14531)
                     /locus_tag="U2R41_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8889226.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="U2R41_001811"
     CDS             complement(14544..15875)
                     /locus_tag="U2R41_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEM8889227.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="U2R41_001812"
     CDS             complement(15886..17328)
                     /locus_tag="U2R41_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8889228.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="U2R41_001813"
     CDS             complement(17315..18079)
                     /locus_tag="U2R41_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEM8889229.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="U2R41_001814"
     CDS             complement(18183..19058)
                     /locus_tag="U2R41_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8889230.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="U2R41_001815"
     tRNA            complement(19313..19397)
                     /locus_tag="U2R41_001815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="U2R41_001816"
     tRNA            complement(19415..19486)
                     /locus_tag="U2R41_001816"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="U2R41_001817"
     tRNA            complement(19496..19568)
                     /locus_tag="U2R41_001817"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="U2R41_001818"
     tRNA            complement(19630..19714)
                     /locus_tag="U2R41_001818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="U2R41_001819"
     tRNA            complement(19735..19805)
                     /locus_tag="U2R41_001819"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="U2R41_001820"
     tRNA            complement(19851..19922)
                     /locus_tag="U2R41_001820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="U2R41_001821"
     tRNA            complement(19934..20006)
                     /locus_tag="U2R41_001821"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="U2R41_001822"
     tRNA            complement(20009..20082)
                     /locus_tag="U2R41_001822"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="U2R41_001823"
     tRNA            complement(20089..20169)
                     /locus_tag="U2R41_001823"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="U2R41_001824"
     tRNA            complement(20171..20246)
                     /locus_tag="U2R41_001824"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="U2R41_001825"
     tRNA            complement(20282..20354)
                     /locus_tag="U2R41_001825"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="U2R41_001826"
     tRNA            complement(20364..20436)
                     /locus_tag="U2R41_001826"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="U2R41_001827"
     tRNA            complement(20452..20523)
                     /locus_tag="U2R41_001827"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="U2R41_001828"
     tRNA            complement(20538..20627)
                     /locus_tag="U2R41_001828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="U2R41_001829"
     tRNA            complement(20667..20740)
                     /locus_tag="U2R41_001829"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
BASE COUNT         6558 a         4342 c         3291 g         6556 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacat
//
LOCUS       DASHWH010000047        20694 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.050, whole genome shotgun sequence.
ACCESSION   DASHWH010000047 DASHWH010000000
VERSION     DASHWH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.050"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            48..365
                     /locus_tag="U2R41_001830"
     CDS             48..365
                     /locus_tag="U2R41_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM8889231.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(523..687)
                     /locus_tag="U2R41_001831"
     CDS             complement(523..687)
                     /locus_tag="U2R41_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEM8889232.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            789..1310
                     /locus_tag="U2R41_001832"
     CDS             789..1310
                     /locus_tag="U2R41_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEM8889233.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1297..3087
                     /locus_tag="U2R41_001833"
     CDS             1297..3087
                     /locus_tag="U2R41_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM8889234.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKTSLISGLSTYMVA
                     YDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFGKPKRSSINYAPAFSKME
                     PRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTRKQPLIVYITTAGYVLDG
                     PLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPRLWIKANPNICLMNFVGM
                     LDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKRNNKTIDIETLKGKKCVG
                     GFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDNNQERIKAWEKVGDLTII
                     PGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELENAGFETNEIRQGFLSLG
                     GPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWMPSKQSKSRKIDGFAASL
                     NSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            3103..4323
                     /locus_tag="U2R41_001834"
     CDS             3103..4323
                     /locus_tag="U2R41_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8889235.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            4358..5035
                     /locus_tag="U2R41_001835"
     CDS             4358..5035
                     /locus_tag="U2R41_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEM8889236.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            5032..6225
                     /locus_tag="U2R41_001836"
     CDS             5032..6225
                     /locus_tag="U2R41_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM8889237.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            6240..6566
                     /locus_tag="U2R41_001837"
     CDS             6240..6566
                     /locus_tag="U2R41_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEM8889238.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            6570..6905
                     /locus_tag="U2R41_001838"
     CDS             6570..6905
                     /locus_tag="U2R41_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM8889239.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            6902..7297
                     /locus_tag="U2R41_001839"
     CDS             6902..7297
                     /locus_tag="U2R41_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889240.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            7294..7665
                     /locus_tag="U2R41_001840"
     CDS             7294..7665
                     /locus_tag="U2R41_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889241.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            7681..8241
                     /locus_tag="U2R41_001841"
     CDS             7681..8241
                     /locus_tag="U2R41_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8889242.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQPDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            8366..8677
                     /locus_tag="U2R41_001842"
     CDS             8366..8677
                     /locus_tag="U2R41_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889243.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            8701..8892
                     /locus_tag="U2R41_001843"
     CDS             8701..8892
                     /locus_tag="U2R41_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889244.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            8892..12740
                     /locus_tag="U2R41_001844"
     CDS             8892..12740
                     /locus_tag="U2R41_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEM8889245.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQSATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            12741..13613
                     /locus_tag="U2R41_001845"
     CDS             12741..13613
                     /locus_tag="U2R41_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEM8889246.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            13610..14659
                     /locus_tag="U2R41_001846"
     CDS             13610..14659
                     /locus_tag="U2R41_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889247.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            14663..15487
                     /locus_tag="U2R41_001847"
     CDS             14663..15487
                     /locus_tag="U2R41_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889248.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15505..17565
                     /locus_tag="U2R41_001848"
     CDS             15505..17565
                     /locus_tag="U2R41_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM8889249.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYGTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            17582..17731
                     /locus_tag="U2R41_001849"
     CDS             17582..17731
                     /locus_tag="U2R41_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889250.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            17728..18174
                     /locus_tag="U2R41_001850"
     CDS             17728..18174
                     /locus_tag="U2R41_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889251.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18176..18313
                     /locus_tag="U2R41_001851"
     CDS             18176..18313
                     /locus_tag="U2R41_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM8889252.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            18348..18590
                     /locus_tag="U2R41_001852"
     CDS             18348..18590
                     /locus_tag="U2R41_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEM8889253.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            18606..18803
                     /locus_tag="U2R41_001853"
     CDS             18606..18803
                     /locus_tag="U2R41_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8889254.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            18814..19830
                     /locus_tag="U2R41_001854"
     CDS             18814..19830
                     /locus_tag="U2R41_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEM8889255.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGISKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANP
                     TLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            20020..20093
                     /locus_tag="U2R41_001855"
     tRNA            20020..20093
                     /locus_tag="U2R41_001855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20054..20056,aa:Ile,seq:cat)
BASE COUNT         7099 a         3578 c         4594 g         5423 t
ORIGIN      
        1 ataagtatcc agatataaaa acaatcggac acaagagaga tatgcaaatg aaagaagcta
       61 gacctagaga cgagatagac aaactataca agaccaaacg atggcgagac ctaaggcaag
      121 tagtaatagc tagggacttc ggcatgtgcc aagagtgcaa gcgtcgaggg cggaacacaa
      181 ggggcacgat catccatcac atagtcgagg cgagggaaga cctgtcactg ttctggtccg
      241 tagataacct tgaatgtatc tgtgtagctt gtcacaacag agagcatcca gagaggtcag
      301 gcgggaagaa gaaaccaaaa cctaaatcac atatcgttaa aatgtattca actcctgaaa
      361 gataagtttg cagtgaaatg aaggcagccc cccctactct aaaagattaa agagtaaggc
      421 ttgagaagaa cggtgctgtc cttccttcgt aaaaagaccg cttttcaagt tttttggaga
      481 aaaaggaaaa agccgaccaa tttaagccgg ctttggacga agctatttct tagtccattt
      541 gtttcctttt tgagaagtag ggggtaatcg gtcgcctgga tcaattttta cttcttgtcc
      601 gccttggaca tttccaccac gaggacccac ttctttgtag gttccttttg gtttgttgtc
      661 ttcgccgggt ttatagagtt ctcccatagg aatccctcct taacaaattt cggcacagca
      721 ctgccgataa cctaattata aggattgtga taacgatttc aatctatctt ttgaaagaag
      781 gtgatattat gccgcaacca gcgaagagtg caaaattaca attattaaac ggaaacccaa
      841 ataagaagaa taccgaagaa ctccgcaagc gagcggccgc agaagacaaa ttaaaaatgg
      901 ctactgacaa aatcaaaccg ccgttatggc tagattcgct aggaaaggat acctttgagt
      961 ttatcgccga tgaattgctg tctgtggatt taatcagtaa tccggacgtc catacaatgg
     1021 ctctatactc caattggtat tcgcaatacg tttctttaga aaaacagctt cgaaaactac
     1081 aacgagagta caagttgaac tatgcgcttg cgaaaaagga ggcagaggcg agaggtgagc
     1141 cgtttaatga acctaatgaa ttaattggta atccgctctc tcggcagatg gatacagcgt
     1201 ctcggaatct ccgttctttt ggcgctgatt taggactatc accagcagcc agagctaagt
     1261 tagctattaa gatggctgat gatggtggtg atgacgatga cgacttctaa tattttggat
     1321 atgtcttaca cagaacgtgt ggactattgg caaagctatc ttgaggagca agcttcttgg
     1381 ggtggctttt taaaatgtcc atatccggaa ttgttaacta cttggtatgc ggaacgatta
     1441 atcgatggaa gcataccagc cagcaaagaa aatattcaag ctgctaaacg gcatatgcgt
     1501 gatttgcagc gccaaggaac agatgatttt ccttggatct ttgacgaaga aaaaggtcac
     1561 cggcctatta gatatatcga aaaaaaatgt aaaccaactg aaggcgactt tggttcgttt
     1621 gttttgcaac cttggcagca tttcataatt ggatccatgt acggatgggt acatcgtgat
     1681 acaggagagc gtcgcttccg cgaggctctt atttttgttg gacgtaaaaa cgggaagaca
     1741 agtcttatct cgggcctttc cacatacatg gtcgcttatg atgatgaaca aggcgccaac
     1801 gtttacgtat tggcaaatgc tcgtgatcaa gcaagcttgt tgtttgataa ggccgcagaa
     1861 atggtcaaac aatcgccggc gctctttaag aaatttggta agcctaaacg atcaagtatt
     1921 aattatgctc ccgccttttc taaaatggaa ccacgcgcct cagatagccg gaaattggat
     1981 gggctaaaca ctcactttgg tatttttgac gagatccacg agtttacgaa ttacaatctg
     2041 atcaacgtta tcaagaaatc aagaggaacc agaaaacagc ctctgatagt ttatatcaca
     2101 actgctggat atgtattaga tggtccgttg atgtcttatt ttgagcaagg tgtggactgt
     2161 ttggaacatt tggaagatga catcgatgaa cggactttct attatctggc aaaacttgac
     2221 agtgcggaag aggctgatga cccaagatta tggatcaaag ccaatccgaa tatttgtcta
     2281 atgaattttg ttggcatgct agatgactat gttaaggata aaaaagatcc aaaagaatat
     2341 gctgactgga ttaccaagca atttaacttg ttttccgata tcgatgagct gtcatttgtc
     2401 gatatgccta ccattaaacg aaacaataaa accatcgata ttgaaacgct caaaggtaag
     2461 aagtgtgtcg gtggttttga cttgtccgaa acggaagact ttaccgcagc cgttttagaa
     2521 tttccgcttg aaacaggcga ggtattcatt ttgcaacaca catggatccc acaagctaga
     2581 tttgatcgag ataacaatca agagcgtatc aaagcgtggg agaaggtggg agatctaacg
     2641 attattcctg gtgattacgt caattatgaa tacgtcttaa attggtttgt agaaaattcg
     2701 aaaatctatg acattgtaaa aatcaattat gacaaggcca aggcgctacg attaaataaa
     2761 gaactagaaa atgcaggatt tgaaaccaac gagattcggc aagggtttct atcattaggt
     2821 gggccaatgc aaaacttcaa ggaaatgcta ttggacggta aggtgatttt caacaattcc
     2881 aagctttacc gatggtatct atccaacgtc aagctggtga tggatcgcaa ctcaaactgg
     2941 atgccgtcta agcagtccaa gagtagaaaa atagatggtt ttgcagcaag tttgaacagc
     3001 cacgccgaag tgttgaatat gttggttaat cctgtcggaa ccgggaaagt aacctattac
     3061 tcgatttccg atttaatgaa tatgtaagaa aggtgtggag gaatgagtat tttagatcgt
     3121 ttgcgttctt ttggccgagc gaagccgaaa gcgagcaaac aagagtattt tttgaatgac
     3181 ccgggattga taccgtattt agtcggaaaa gatgaaatat cagaagggat tttttccgta
     3241 attagccgtg tatcgaacgt ttttgcgtct ctccctctca aaatgataga tgtggagttt
     3301 ggccaaccgg acgactgtcc tgcatacaac ttgttgagcg aaggccctcg atattttaca
     3361 aagtttgatt ttttccggga cgtggaagtt ttgagaaact accaagggaa tgcgtatgtg
     3421 cagattttcc gaaatatcaa tggagaagta gcagatatgg cgttagtaaa acctggtgct
     3481 tgccatccag tgattgatat ggatagcggg gagctttact accaagtaac tgcgactgac
     3541 aaaggcagtt acaagcaagt tatctatgta cattacatgg aaatgcttca ctttaaacaa
     3601 ccgaggtttg gcggcttgga aggtacagac cccacaaaag tattaacgaa taccctcgga
     3661 tatgatcgag aagtccgaaa aatctcttta agtcagctta aaggaagtaa tgaagggcta
     3721 aaagttaagt ttgctagcaa tatggatgaa gaagctaaaa aagctacagt taaaaacatt
     3781 gctgattttt atcgacaaaa cggtggacta cttgtggaag aaaacggtgt agaaatcgaa
     3841 cgtttacaac gagagctggt agacagcaag cttttagata ctgataaagt atctcgctcc
     3901 agaatcgcta tggtctacaa tgtgccggag catttcatcg ggaataacca gtcgagttac
     3961 tcctctcagg aacagctcaa tatggaattt ttgacgtaca atctagtacc gaccgttaat
     4021 caatatgaag cggaactaaa taagaaaaca ctatcgagaa ccgaaaaagc aaaaggttat
     4081 cgatataagt ttaatgtcgc aagtttgcta agagctgaca ctcaggcaag gggacaattc
     4141 tatcaaatca tgcgacgagg cggagcatac tcagcgaatg atgtccgaag atttgaggac
     4201 ttgcaaccga taaataaaac cggtatggat gattaccata tttccggaga cctatatcca
     4261 atcgatatgg atccaacatt aagaaaaaca acctcgtcta aaagcgtagc cgaaaacggt
     4321 taggtttttt tagtttgcac cgaagggagg tggaaaaatg aaaaaagtga cgttaagcgg
     4381 cgatgtcgtg gataacgata ccgcgtggct ttatgactgg tttgggatca attgtatctc
     4441 accagggaaa atttctgccg ctcttacaga agcagcgggg gatgaagtag aacttgatat
     4501 ctcatcgaac ggtggggatg tcctagcggc aagcgaaata tataccgcta tccgtgccta
     4561 tccgggaaag gtatccggaa atgttgtgag tattgcagca agcgctgcaa gtgtaatcgc
     4621 ttgcgcttgc gaaccgctta gaatctcacc tacggcacac atcatgattc ataacgcatg
     4681 ggtgaccact agtggcaacg ctgaggaatt aaaagccaat gcagaaatgt taagcagtgt
     4741 ggatgagtct attgttaatg cttacgagat caaaacagga ctcgatcgga aaaaacttgc
     4801 tgatttaatg gcgaaagata cttggttaaa tgctcaaaca gcagtagcag aagggtttgc
     4861 agatgaaatt atgtttgcag aagcaccagt aacggtactc aatgcctctc aaccggttat
     4921 tccaaaaaac gcagtaacta agttgaaaaa tttaatactc aaagcggaag caccgcaaga
     4981 agaaacactc ttacagaaaa aactaaaagc cttaaatgga gggaaaaacg aatgaattta
     5041 gaacaattaa aaaatgcgtg ggtcgaggcg ggaagtaaag tctctgactt aaatgcacaa
     5101 ctcaatgcag cattggttga cgatgaaaaa acagaagaag atgtagtaag tttgcaagca
     5161 caagtaaaag cagcacgggc taaacgggac ggattgaaag agcaagtggc aaatatggaa
     5221 gccgaacaag tcttaaacgt caaaaaagaa ccattagata aaaaagatga aaacttgaaa
     5281 aacaagttta tcaaagactt taaagcgatg gtcaatggtg atcctgctat tatggctact
     5341 ttgacatcta atacggatga atctggtaat gctatcggat tgactattcc tgtagatgtg
     5401 caaacgacta ttcatacttt ggttcgtcgg tttgactctt tgcaagaata cgtaaacgtt
     5461 gaaaaagtga ccactaccag cggttctcgc gtttatgaaa aatggtctga tattacaccg
     5521 ttgactgctt tggatactga agacggtgaa atcccagcaa atgatgatcc tgcactttac
     5581 ttgatcaaat acttgatcaa acgctatgca ggtatttcca cagtaactaa cagcttgcta
     5641 aaagatactg ccgaaaacat tttggcatgg ttgtctaaat ggatcgcgaa aaaagtagtt
     5701 gttactcgca atacaaaaat cttggcagct attgatggaa tcaaagcggc acaaaagaaa
     5761 gatgttacag atgttgatgg aattaaagat atcgtaaacg tccaacttga tccagctatc
     5821 gaagctacat ctatgtttat tacaaaccaa gatggcttca atgttttaga taaagtgaaa
     5881 cgtgctgatg gatcttactt gttacaaaaa gacgtagctt ctgcaactgg atataccttc
     5941 ttaggtaaac caatcaagaa aattgcttct cgtttcttgc caaacaaagg tacgcaagct
     6001 actcctaaat atccactgta cattggtgat ctgaaagaag ccgttacatt gtatgatcgc
     6061 gaaaacatga gcttgctgac aacgaatatt ggtggtggag cttttgaaac agacaccact
     6121 aaagtacgcg tcatcgatcg cttcgatgtg caactagttg atgatgaagc ggttgttttg
     6181 gctactttta caactattgc gaacgagaca ccggcgaaag tttaaggagc tgattcctta
     6241 tgattcttga tcctaaaatg gatttaggcg aaatcaaaaa cgcactaaag attgataccg
     6301 atgatgacga tgtggaagta agccgtgcgg cacaagctgc aattgcatac attagagggg
     6361 ctatcgggaa tgataagccc tctttttata agcaagaaaa tgaaacggtt gatctgatta
     6421 atttagctat cctgcaatta gcagatcatt attacaacgc tcgatcagca accgtgagtg
     6481 ggaacttgcg agagtacgat ttaggtttta caagcctaat cttgcaactc aaagcaagtt
     6541 atttgctttt tgtggaggag gagtagcgta tgccccttat ccaaacagga aatttaaatc
     6601 aacgcatcaa gtttgtccga gatacgacgg ttaaggatga ggacgggcaa gttgtcccga
     6661 cttctacaac cattcttact tgctgggcaa gtgtgcagac acaacgcctg aacgatatta
     6721 agacgtcgat tggtacggct ttggaaggaa cactgacgtt cattatccgc taccaacaaa
     6781 aatcagagct gaccaatgat atgaaagtgc gttggaatgg aaaaacgttt gaaatcatta
     6841 cgattacgaa aggcgagttt gcgaaggact tcacgacaat cattgcaaaa gaggtttcaa
     6901 aatgagtgta gaagtcgatg caaccgaagt gtacaaagcg cttagggaag taaaagcaaa
     6961 cgttcaacga gtggaaagcc cagcacttag aaaagctggg gagtacgctc aagaagagtt
     7021 acgacaaaac acaccttact gggatggaac gaagtcaaac ggtaaacgtg gttcgtatat
     7081 gcaagaacat gctaagaacc atgtggttac aagctcgata aaaaacggat tggtagaagt
     7141 cggctatgac aaagatgttt cttggcggat gcactttatc gagttcggaa caatcaaaca
     7201 acgtccaaaa ggtttcgtac aaaaaacaca aaagcaaatc gaaaaacaag taacacaaat
     7261 cattgctgac gaagtaaaaa ggaggctagg actttgaaaa cggcagtatc acaagtctat
     7321 tcaattctga atagcaatga aaaaacaaag aacattgatt tttacaccaa tagtgttccg
     7381 gaatcagctc aaacagtacc tagccttcca gttggcagaa ttacagagat atccggcaac
     7441 tatgaagatt ttgcaagcaa caatcctttg accattcaat ttaacgtaca ggtagatgta
     7501 tgggtgtcaa ccatgaaaga ggttgatgcc ttttattttg cccttgatga ggttatgagg
     7561 gggaatggtt ggcaatgcgc atacacggaa caaacagatg acgaggactt ggaaggtgca
     7621 aagcggatta tcaaacgata tgtagcaaat atttcactaa attaaaagga gagaaaatag
     7681 atggcaacag taggattcga gagcgtcatt tttggcgtaa aaacaggtgc aggcggcact
     7741 ctaaaagaat tagtagcaga taagtcgaaa ggcggagcga tcgaagctaa aattactgga
     7801 ttaggcgcaa cttctaacac aacatacgct tcaaacgtac cgttcttcat tgcaagtaaa
     7861 ggggtttcgt cgccaaaagt tacgcttgac gtggcagact taatggataa cggcatttac
     7921 agcgaaatca ttggtgctaa aaccgtggat ggtgcaaatg taattggttc agaaactgaa
     7981 gcgccttacg tgtcggtagt catggttaca gcgaacaaag aaggaaaacg cttgttcatg
     8041 ggattgacaa aaggaaaatt cagtcatcca gatatcgaca tgaaaacagc tgaagacaaa
     8101 ggggtagaat tgcaacccga ttccatcgaa ggggaattca tttctgatga acgtggctat
     8161 gtatacttaa cagccgtaga atcagaagga atgaccttac aaaaattcaa ggacttggta
     8221 aataacaaag agggggagta gttaaccctg catctacacc aacgacagat acagggacac
     8281 caaaagaacc agaaccaaaa attgatacac aaggttagcc atttttggct agccttattt
     8341 tttgtaaaaa caaggaggaa aacaaatgat tgaattgcaa ttgaaacttg acggaaagaa
     8401 aaaaacattc aaacaacaag atatttccgc acgtgcaatg cgtgagtgta tcaaatttta
     8461 cgagaaagcg gaaaaagcag acctaactga tttagaagcg attgattcaa tgattgcaat
     8521 tacagcagat attttccaag atccagcagt tacatttgat gctattttag acggtttgac
     8581 tgcgagcgag ttagtaccgg cattagaaag tgtttttgaa caaataaatg aactgggaaa
     8641 caatgaaaaa aagcagacgg cgagcaaaaa gaaataagtt tttctgaagc taggaaagca
     8701 atggatcaaa tctacaaaga tttaatcgaa gcaggttgga cgatgagaga tgtggacgaa
     8761 gccgactatc attatttgtt acaccttttt ggagaagtgg agagtggcga agaatatgta
     8821 gatggtgctg atttcatcaa acaattttta tcggctgaag acttagtaaa acttgaggaa
     8881 ggaggtaaat aatggcagga aaaggacaac cggcaggaaa tatcaagcta gggattagtt
     8941 tagatagcac tagttttggt aacacgctgg acgaaatcaa tgcgaaagtc aaacaagctg
     9001 agtcgaatat gcgtgccaat ctaaaggctt atgattcagc aggacgttca tacgaagcac
     9061 ttagtcaaaa gacgaaagac ttgtctacgg ttatggaagg gcaaaacgcc aaagtaagag
     9121 aattaacaaa gcgccgtgat gaagcgatta gcaagtatgg cgaggaatcg aaacaagttg
     9181 ctaaccttaa cacacagata aacaatgcta ccgcaaaata taatgcttac agtcgccagt
     9241 tgaacgacac aaaaaaagaa ttggtgtatt ccaaaacagc cgtcaatgat ttatctaatg
     9301 aaatcaaaga aaatgaacga caaatgaacg ccgaagtaaa agcgttgaaa gccgctggtg
     9361 atgaatctgg tgcttttgaa gcaaaacaaa aagggctagc caaacaaacg gaattatccg
     9421 agaaagctat cgaagaacag cgcaaagttg tgaaactgat ggctgatgag tttggcgatt
     9481 cagcaaatga aaccgaagat gcaaaaaggg cattagaaaa gttagaacga caaagccaaa
     9541 tatctagcag gcaattagaa gcactcaaaa gctccagcga tcaatcagga aaagaaatag
     9601 aagattttgg cgacaagtcc acaaggtcag ctaggaaact ggacggacta aaagacaaat
     9661 taagctcgct aaaaagcgca ttttcgtttg gtgcagttgc tggattagcg cataacgcta
     9721 ttagcagtgt agtaagtggc gtgcaaggtt tggttggcga agcagtaaac gcatcggatt
     9781 cattgatgaa gttttccaaa accatggagt ttgctaactt tgggaagtca cagatagaaa
     9841 gctcgaaaaa agaaatgaaa gactacgccg ataagacggt ttatggttta gaagaaattc
     9901 tgaacacaac cgcacaattg gcatctaatg ggattcctaa ctatacagaa ctaaccaagg
     9961 cggcaggtaa cttgaatgcc gttgcaggcg gttctagtga tacattcaaa tccgttgcca
    10021 tgatgctaac gcagacggca ggagctggga aactaacaac tgaaaactgg aatcaattag
    10081 cagatgcgat accgggtgct tcaggattgt tacaagacgc tatgttgaaa aacggagctt
    10141 atacaggaaa cttccgtgat gcaatggcgc aaggtcaaat cacttccgac gagttcaacc
    10201 aagcaattgt acagttaggt atgaatgacg gagcagttaa ggcagccact tctacagaca
    10261 cattgagcgg ttcttgggag cagatgaaat ccactgtaat aaatgggcta caaagtatta
    10321 tagaaaaaat aggcgttgaa aatatcactg gtttcatcaa cagagtaaca aaagggattg
    10381 aaaattctat tcctaaaatt actcaattta taggttggtt gagagatatt ggaacgtgga
    10441 tcgttgaaaa tagagagcca ctaacatgga ttgtcggaat cataggcgga attacattag
    10501 cagtaaaagc attgaacgta gcaagtatgt tgctggcaat tactggcgga acattggcag
    10561 ccccttttgt ggcgattggt gtagcattag gcgcactagc aggtgctttg gtgatagctt
    10621 atacaaaatc tgaaacattt agaaatatag tcaatgcggc ttttacagct gtgaaaaacg
    10681 tagttatgag cgttatcaat aatttggtgg aatactacaa aatgttgtgg agcgtgttgc
    10741 agtggctttg ggaaaaaata aaagaatggg cttcatggat tggcaataaa ttcattgaaa
    10801 tgaagaacag cgttgtgaat acggttcaga acctatggaa tggcgtgaaa aacttcttca
    10861 gcaatggcgt tggagatacg tggaataagg tagtcggttg ggtaaaaaac attttcaaca
    10921 aagcaactga attgaagaac aacgtttctg atgtaatcgg taacctgtgg aatggtatca
    10981 aagacacatt ccgtagaggt atcgatacgg tattcaattg gttttcagaa ctaccaacga
    11041 agatgaagaa tgccattatt ggcggtaaaa acgccattgt tgatgcgttc aaaagtattt
    11101 tcaacgcagc acttaaagcg ataggtaaac cagttaacgc aatcatccat ggagcttcat
    11161 gggtactaga aaaattgggc gcagaacctc tacaagaatg ggatgtacca caatacgcta
    11221 caggtacacc agcaggtggg cacccaatca atggtccaat gatggttaat gatggacgtg
    11281 gagcagaaac agttatcaca ccagatggta gagccttcat tcctaaagga cgcaatgttg
    11341 ttctaaacgc accaaaagga acgcatgtct tgacagcaga agagaccgcg caacttcaag
    11401 gttctaaagc tcctaagtat cgttacaaaa aaggtactaa cttctttggt aacatgtggg
    11461 atagtgtgaa aaaggttgct ggtaatgtag gtcacacaat taaaaacgta gtaggtgacg
    11521 tgtgggactt tatttcagac ccgggagcgt tagctagaaa agtacttggg gggttagatg
    11581 tattaggtgg attgacaaag tatccattag aagtaggtaa aggcatccta tctaaagcaa
    11641 caagtgcact gactgaaaaa atcactgggt tgttctcatc tggtaactta gatacctccg
    11701 taggaacaaa tggcgtctat aaatatttgg ctgatgttgc taagtctgtg atgaagaaat
    11761 tcccaggatt tgtggcaact agtgggtata gaccaggtga cccttattca catggtaaac
    11821 gtaatgccat tgatattgca ttaccaggcg tcacaggagg ctcacctaga tacacagaag
    11881 cagcaaacta cgcttttgac aaattcgctt ccaaaattgg ttacgtaatc actaatggga
    11941 aagttcgtga ccgttcagga caatcaggtc aaccagcaac tggtgcatgg gagccatggc
    12001 ccgatggaga tcactatgat catgtgcatt taaacggtgt gaaagaccca caaaacactc
    12061 aaatttcagg agatagcgtg ggaggcagtg gggtagaaag atggcgcaat gtagcaatta
    12121 gagcgttgaa aatgaccggt caatacagta ctgcaaactt aaatgcatta ctaaatcaaa
    12181 tgcgtacaga gtcaaatggt aatcctaatg cagttaacaa ttgggatatt aacgccaaaa
    12241 atggaacacc atcaaaaggg ttgctccaag tgattgaccc aacattcaga cagtatgcaa
    12301 tgccaggatt caacagcaat atttttgacc cactatctaa catcttagct tcaatcagat
    12361 acgcactatc aagatatggc tcactaacaa atgcctatcg tggagttggt tacgcaaacg
    12421 gtggaattgt aaaccaacat caaattgcgg aaatcgcaga aggaaacaag ccagaaatta
    12481 ttattccgtt agataaggct aaacgatcaa gagcgatgca attattgtct atcgcaatgg
    12541 acaaactagg agtttctcct aaaacttatg gaaatgcgac aactgtttcc agtgattacg
    12601 aaatcctaga agccattgaa aaacagtcgg caacaacgaa tcagctgtta tcgttattgc
    12661 tggcattttt caaaggaaat agccgaactg atagagattt agctttagat attcagaaga
    12721 tcttggttag gaggatgtag atgcgaactg tattactgag aaatagaata aacgaagaaa
    12781 ttgatttgtc cacagaagac tattttgcta ctgggttgag taatatgggg tttgaagtaa
    12841 aaaaagaaca tgtgggacag tgggggaatt tcagagaaag cagtgagagc gttgaaatat
    12901 ctgaatttca gtcatctgta atcatttctg tgcatgggtt tcgagaaaaa gaactgtaca
    12961 attcacttgt gcagttccta tcggaaggtc cgtttgaact ggaatttgct tttgacggcg
    13021 aaacaatggt aagaagatgc agtctaaaat ctttaagtaa gactgaaatc gatccgaaaa
    13081 catcgttact aacagacact ttagaactat atttcacatc aaactggtat tccgtaaaac
    13141 gcgaaaaatt aattcagcga ccgaatgtag tgaaaacacg tggtaaggtt tttccgtata
    13201 aaagatctta tatctacact caaaacttgt gggagaaaaa aggtgtattc aaattcaata
    13261 ataattctgt atacctaacg aatagcaagg aacggatgtc cccgctaaaa attcgtgtga
    13321 ttgggaagtg ttcaaatccg tattgggaag taatccaaaa ctcacaaatc atcgcaacgg
    13381 atggatactt catagatatg actgaaacac agactctaga agtatcaagc ctttttgaag
    13441 atacaacggc gattttaaaa gatattgcag gggtagaatc ttctgtctac caacaacagg
    13501 attatacaaa aactaatttt gttcaggccc caactggaga attcagcatt gtgtttcatg
    13561 ttgggggggc agacgtagag attgagttat acgaggagcg tgatctgttt tgattttagc
    13621 agtcacgctt tttcatcgtg atttaatgct ttataacgaa catttattct ctaacagttt
    13681 tgaatttggt gtggacgaga tcaacgaaga ggctagtagc tttacgatgg ataagtacgt
    13741 tcctgtaaaa acaggcgatt ttctactagc aaaatatatt cctagtggaa aatttgcata
    13801 ttttggtgta attacgtcgc aggaagacga aaaaatcagt tgtaaaagct tacttagttt
    13861 agctgatagt gagataccga ctgcacgtgt gtcaggagat aactacgaag agcatatccg
    13921 gcggttgatt gaatactatt tgctgaatga tccaacgaaa caactaaaag atattttaga
    13981 cgtcaaagca gaaagtgcga cctctcattc gtatcaagct accgatacga ataaacacaa
    14041 gttaagtgcg tatattctca atggtttcaa aaaatacaac gtaaaatggt atttcaaggg
    14101 aattcaaaac agaaaaattt atacaggcat acgtgctgta aatgaatcaa tttacattaa
    14161 agacaattct tctgaattta gcgattggga tgtgtttgtt caggcgccgg gcgctggaaa
    14221 cgagaacaag ctattaatcg ttgataaagc aatgaaagat atagagaaac cgataatact
    14281 gtcaacatgg tatttggacg aagaaaacaa tttgacacaa gatggatcga aagaaaatat
    14341 cacgaaacca actgtcaatt tggttaatat ctacgatcaa accgcagaag ataaagcatc
    14401 ctacgaagat gtggcaaaat cagagttgaa aggcaatacg tattcgcatg aaatcaaagt
    14461 gaacgttgta agaaatgcaa aaaatttgaa tgtcgaaacg attgaaacag ggatgtttgc
    14521 cacgatttct tataaaggaa agatatacaa gtcggttttg acagcttggc gaatatcaag
    14581 tgataaggag tttgtggaat tgactttcgg aaacataaga agtcgtttta tggattattt
    14641 tgaagataat gggggataaa aaatggttag caatgtggat ggatatcaat ttgaaaacgt
    14701 gaaagtaagc gcagaaaatg atgctagact ttatcacgtt ttatataatc ggaaaaatca
    14761 ggttattgat ggttacgatc agtctatgaa tttatcttca agcggattaa cagtaaaagt
    14821 tgctgcagga gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag aagaaagtat
    14881 aacagttcca gcaaactcaa gtggttatat agcattaaca gtggatttga cacaagaagt
    14941 tatacctgga tctattcttc cagaatcgga agaatatgaa tggactaata atcaagtcaa
    15001 gctagaattt ataacaaaag ttataaaagg taatttaaac aatggtgata aggtctataa
    15061 tttaccacta tgctcagtta attctactgg atcaactgtt tcaatctcaa agatatcgga
    15121 tagttacgag ctgactctct ctaaaggaga aattttgtgg agggggactg cgttaatgca
    15181 tgatactcaa actgtccaac cttcaaaaaa aatttggcag acagtttcag gttttttgtt
    15241 agtatggctc ccatacgaaa acgggcaagc aattgaggat agatatgtaa ctacgccttt
    15301 ttataaggag cgtgtaactt ttactaatgt tttaggagaa attgtttcag gatttgatga
    15361 ctatcacaaa aagtggttta gtaaacgaat aaactataat tcgaatacta atatatttac
    15421 aggtgcagca agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt atatagtttc
    15481 tttttagtta ggaggtatgg aagagtggca aatttagaaa ttaaattatc tgcaaataag
    15541 agacagcctt atctacgtca ccgtgttgtt ggtagagttg gtgatggggg gctcacaaca
    15601 atcaatgtac aacttcttga ggaagatgaa attacacctt ttgtaattaa tctaaacggt
    15661 actttgaaat ttgtgggcga agtttcaaac ggtaactata ccgagggaga accagaaata
    15721 atcgattcga ctaatgggtt aattagttac acgttcacta agtcaaattt cagcacgagt
    15781 catcaattca aacaagcata ttttgaatat gtagatccta acggcaaaaa agtaactttt
    15841 cagaacttca tcatagacgt gttagaacga gtggatatta attcggagca agcgaaatac
    15901 tatatttctt cattggaaaa attacagagt gaaatgcaaa ccactttcaa tcagttcatt
    15961 agtgataaac aggtccaata cgatcaaatc tactcgaaat ataacgaatt agtaagattg
    16021 ataaatgaat cagataagca agtaaatgat cgtattgatc aaaccaatca gcaaatcggc
    16081 gatctcggca agctgaaaaa gatgtacagt aacagcatcg actttggggg ctatgattat
    16141 tcggggaatc cgaatttgtt aagtaagcta tcatacgact taattgaaaa tcaaaatact
    16201 tcagctggaa cactttctaa gggtgaaaac tcgtttaaat ataataagat atcagctgaa
    16261 gtggaaggtg gagtagagtt atattataaa cgaagaggta tagctaactg gttaccctct
    16321 aataaaacgc ttgtaatgac tgttaagctt agagctggag cggactatag tccagttgac
    16381 ggaaaactta tactgattag atataggtat gttgacagtg gaactggcaa gattgtttta
    16441 gacttaccta ttaacagtaa ttcgataact caggaatgga aagagtttag tattactgga
    16501 actactccaa catttagccc acaagcatac catccttgga tacaatttag ggctcaagat
    16561 ggggtacttg gggaaataga aatgagctat gacatcaaaa tcgaagaagg ctctacagct
    16621 acaccatatc agcctaactt acttgatgac tcttactggc taggtaaaac accgttgggt
    16681 gagaatttga taacgaatgc caagttccca attataacac gaagcaatcc tatttctggt
    16741 tttgatattt cagaagaatt aataataggt gaaacttata cggtatcttt aaaagggact
    16801 aagccagcta ctaaagaatt ccacttatac tacggagaag caaattacca gcagttgcag
    16861 tatcaggcaa ctttacttcc tgtggaagga ttaacagatg tgtggagtgc cacttttaca
    16921 gccagaaata ctagtgaaac gacaaattta cttagagtag cgttatggca aaaacctaat
    16981 tctgcaacat atggtactgt tcaaattgac tggctcaaga ttgaaaaagg cgacacacgc
    17041 acaccaaata tcagtcagtt taaatacttt ggtgaaggct tgaaagacag taacaatccg
    17101 aacgactaca gctgggatgt cacaccagag tatactgaaa aaagcttgaa taatacggtt
    17161 agtctgactg aaccgcaatc catcgaagga ttgaaaaatt tcgaagatgg tattcaatca
    17221 aaaggaaaat ctgtattgac atcagacgac aacaaatatg aagtcgtaac cttaacagtt
    17281 acaaacggga ataccggatc agcaaagctt tatcgtgaag gaaaaaccgt cactatttat
    17341 ttttttgcgt tgaatgggaa aagcagtggt ggaaatgatt caacgatact aacaattcca
    17401 gaaggctatc ggccaccaat tagttttgag caactggttg gctcgataga ccgttctact
    17461 ttgaacagtg ctcagttatc tattggtgca gatggagcca ttaaatggcg aagaaactca
    17521 agttatggat cggattatac ctttgcaatt acttacacga tttagaaagg cgtgaatcga
    17581 tatgaaggca gcatatagac caattgaacc ttacggattc gagcaaatca ttgtgaatga
    17641 tgaagaacat ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc
    17701 gaaattcaat tactgggagg gaaataaatg aaaaacattt ggaaatatgg ccgtactggc
    17761 ggagagtatg caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag
    17821 ccaccgctcg aaggggttcg ttcagatggc gaaccgctaa cgattgctga tcagatgttt
    17881 gatcctaaat tgaaccaatg gattatttta gcgaacgcgt tagatcacaa cgatttaaac
    17941 aatctcaaag cgatgtacga ggctcttgaa catgaaaacg gcaacctaaa acagctaaat
    18001 gccaaactca tgctaagcga tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa
    18061 gctgacagtt tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa
    18121 gacatttcag aaattaaaga gcaactaaat ccagcttcaa agggaggtga gtagtatgtt
    18181 tagttttagc gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg
    18241 tctattcgtt ccactatgta tcacagatga agaagcagat agaattatta gcaaagaaga
    18301 gagcgcatct taagtgatgc gtttttttgt tggaaagttg gtggaacatg aaagaagaag
    18361 cgctccaaga cgttgtggag agattagtaa gaattgaaac aaaattagac aactacgaat
    18421 cacttagaga aaaagctgat agtgcaaaag atttggcaga caaagcctac tcagtagcgc
    18481 taaacaacgc agaagatatc aaagaaatga aagccaataa taaatggtcg tggggttaca
    18541 tgatcggttt aggcattacg atcattggct atttcttgac taaattgtaa aggagttaag
    18601 aagaaatgat tttacccgat aagtattatc aagtcattaa atggacggtt ttaacagtat
    18661 taccagctgc atctgtttta gtagccacgt taggaaaagc gtatggatgg aatggaacag
    18721 atatgacagt actcactatc aatgcagtag caacattttt aggcgttatc actggtgtgt
    18781 ctgcatataa cttaaaaaaa taggaggaaa aaaatgaaga agaaaatcat tttatcattg
    18841 agcctaataa tggctctttt tttattgcca attaacgggt tcgcctatac gatcaacaat
    18901 gaatttaatt taggtgtaaa tgaaggtagc tcacaagtag caaataatca gtatatttta
    18961 ctgcatgaaa cggctaatga aacagcgaca ggacgcaatg aagcacagta tatgcaacgt
    19021 tcatggacta gtgcttatac tgcttacatt gtgggagacg gcggaattgt ttatcaagtc
    19081 ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa tagtcctgtg
    19141 cagattgagt tacaacacac acatgataaa gcaacttttg aaaagaacta taaagcatac
    19201 gttgaattgg ctagagattc agcaatgaaa tatggtattc cattaacatt agacactcct
    19261 tataaccaac caggaatcaa atcgcattta tgggtaacgc aaaatatttg gggcgatcat
    19321 acagatcctt acggttatct ttctgaaatg ggcataagta aagaaaaatt agcatatgat
    19381 ttagctcatg gatttaccga tgaaaatcca actacttcag atgataaacc agtcattgat
    19441 ccaactagag caggtgctgc aaatcctacg ctgacagatg gaacaaatta cgcccacatt
    19501 gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag caggatggca tattgctaac
    19561 tataaatacg agtatatttt cattatggat tacaatactg gaaaagaatt agctcgagta
    19621 aaagctgatg ggatttatag accagacgta aatcaagctt ataatacttc tggaaatgtt
    19681 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
    19741 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
    19801 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
    19861 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
    19921 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
    19981 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
    20041 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
    20101 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
    20161 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
    20221 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
    20281 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
    20341 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
    20401 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
    20461 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
    20521 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
    20581 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
    20641 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ctggcacgtc agtc
//
LOCUS       DASHWH010000048        20522 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.051, whole genome shotgun sequence.
ACCESSION   DASHWH010000048 DASHWH010000000
VERSION     DASHWH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.051"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(436..1749)
                     /locus_tag="U2R41_001856"
     CDS             complement(436..1749)
                     /locus_tag="U2R41_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM8889256.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1994..2218)
                     /locus_tag="U2R41_001857"
     CDS             complement(1994..2218)
                     /locus_tag="U2R41_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889257.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2238..2660)
                     /locus_tag="U2R41_001858"
     CDS             complement(2238..2660)
                     /locus_tag="U2R41_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEM8889258.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(2802..3512)
                     /locus_tag="U2R41_001859"
     CDS             complement(2802..3512)
                     /locus_tag="U2R41_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889259.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3530..4026)
                     /locus_tag="U2R41_001860"
                     /pseudo
     CDS             complement(3530..4026)
                     /locus_tag="U2R41_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4063..4515)
                     /locus_tag="U2R41_001861"
     CDS             complement(4063..4515)
                     /locus_tag="U2R41_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8889260.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4650..5255)
                     /locus_tag="U2R41_001862"
     CDS             complement(4650..5255)
                     /locus_tag="U2R41_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8889261.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5705..6325)
                     /locus_tag="U2R41_001863"
     CDS             complement(5705..6325)
                     /locus_tag="U2R41_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8889262.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6327..7196)
                     /locus_tag="U2R41_001864"
     CDS             complement(6327..7196)
                     /locus_tag="U2R41_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEM8889263.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7219..7626)
                     /locus_tag="U2R41_001865"
     CDS             complement(7219..7626)
                     /locus_tag="U2R41_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM8889264.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7645..8934)
                     /locus_tag="U2R41_001866"
     CDS             complement(7645..8934)
                     /locus_tag="U2R41_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889265.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8961..9263)
                     /locus_tag="U2R41_001867"
     CDS             complement(8961..9263)
                     /locus_tag="U2R41_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889266.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(9288..11381)
                     /locus_tag="U2R41_001868"
     CDS             complement(9288..11381)
                     /locus_tag="U2R41_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8889267.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11870..12598)
                     /locus_tag="U2R41_001869"
     CDS             complement(11870..12598)
                     /locus_tag="U2R41_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEM8889268.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12744..15365)
                     /locus_tag="U2R41_001870"
                     /pseudo
     CDS             complement(12744..15365)
                     /locus_tag="U2R41_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15479..16810)
                     /locus_tag="U2R41_001871"
     CDS             complement(15479..16810)
                     /locus_tag="U2R41_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8889269.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16776..18254)
                     /locus_tag="U2R41_001872"
     CDS             complement(16776..18254)
                     /locus_tag="U2R41_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8889270.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18559..20394)
                     /gene="tdc"
                     /locus_tag="U2R41_001873"
     CDS             complement(18559..20394)
                     /gene="tdc"
                     /locus_tag="U2R41_001873"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM8889271.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5930 a         4407 c         3442 g         6743 t
ORIGIN      
        1 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
       61 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
      121 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt attcagggaa gaatgctgat
      181 ttcacaactt ctttataaac ggtgttcact cagctgtccc tcggtattag ctcaaaaaac
      241 ggcaaaatat aagaaactat ttttttcgtt tttttctcta atactcaggc cagaacactg
      301 atttcacaac ctctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa
      361 aaggcaaaat atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact
      421 gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccatga atacaaagaa
      481 cagaccatac atgatgggag atacttcttt tcctctttta gctgctaaca tggtaattgg
      541 atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat
      601 caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga
      661 ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac
      721 tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt
      781 gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg
      841 agaagtacct aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc
      901 tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt
      961 tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag
     1021 agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc
     1081 tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt
     1141 agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac
     1201 gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa
     1261 tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag caataaatag
     1321 accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc
     1381 ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa
     1441 tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat
     1501 cccaagagca ggtgccgttc caattggata acgggctaat acccccatta aaatacagcc
     1561 gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc
     1621 taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc
     1681 cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt
     1741 ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg
     1801 atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa
     1861 attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt
     1921 catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt
     1981 ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga
     2041 tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca
     2101 tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct
     2161 cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg
     2221 tttctcctcc tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct
     2281 tcagggtccg aaaaattgaa agtcaagacg ccattgttgt ctgtgatttc tcctggttct
     2341 tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt
     2401 cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat
     2461 tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa
     2521 atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa
     2581 agcatcacac gtgttttttg tatcatcgtt tgacccttct ttctatttga ttttctttcc
     2641 tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt
     2701 caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa
     2761 aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat
     2821 tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc
     2881 ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg
     2941 accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg
     3001 agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga
     3061 gcatcggaat ctgcacctaa aggcaggaat gattcgtcac cttgggcttt tcctaaatgc
     3121 agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa
     3181 atactattat agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca
     3241 cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat
     3301 gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt
     3361 cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt
     3421 acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc
     3481 cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat tagaataatt
     3541 gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt
     3601 atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac
     3661 cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa
     3721 atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa
     3781 aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag
     3841 gctcttgatc gatactcact aggaaaaaat ctgtttcttt atcttttagc tcttctgtaa
     3901 gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt aattatcttt
     3961 tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg
     4021 cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt
     4081 gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata
     4141 ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt
     4201 gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt
     4261 gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt
     4321 gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat
     4381 gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac
     4441 ttctttgaat tcgatattgc taatagagtc tagcgctcgg gcaatcattc cgatgtctcg
     4501 aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt tacctctttt
     4561 tgttgcaaat gcaactattt ttaataaaaa aatcaagttc ttttttaata actagtaaag
     4621 tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct
     4681 aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata
     4741 ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg
     4801 atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta
     4861 ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag
     4921 acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt
     4981 gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag
     5041 gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt
     5101 tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc
     5161 tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg
     5221 agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat
     5281 gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa
     5341 atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg
     5401 gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc catcaaacga
     5461 aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt
     5521 cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt
     5581 catcaaacat gagaagctgt ttctttgaaa ttgctccttg tgactcggag ctgaacgaca
     5641 ggctcacgac ctctcggtaa acggtgttca aaagccaatt tttcgatatt aagccatttc
     5701 tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca
     5761 cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt
     5821 ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc
     5881 gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg
     5941 attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa
     6001 tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat
     6061 cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg
     6121 ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt
     6181 taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat
     6241 ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag
     6301 ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag
     6361 ttcataaaca gtccaatctt atgcttgaca atttctttta ctgctttatt tgctggaatc
     6421 acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc
     6481 gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat
     6541 tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact
     6601 actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca
     6661 tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact
     6721 gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta
     6781 ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact
     6841 actgcttttg tcttcaagat attttctttg aaaggtagat gggacccgtc atacatgaca
     6901 gaagtatatc cgctatcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag
     6961 tgtaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca
     7021 tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgattgg cgaacccatt
     7081 tcttttgctg cgcctagcat ggcaggaagc atttctaggt tatgcgtatt aaaagctcca
     7141 actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt
     7201 tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg
     7261 catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc
     7321 atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg
     7381 tttattttcc aagctctttt ctaaatattg gatggcttga tcgttctcat gcaattcaga
     7441 aaatttaagt ccaacttgtt cataaagttc cgctaattta tgcctatctt gggtagtgga
     7501 aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag
     7561 ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc
     7621 aaacatcatt ttccctactt tcaattaaaa cgctttcaaa actgggctta agatccaggc
     7681 atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattga tgaatcccat
     7741 cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat
     7801 accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc
     7861 tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa
     7921 agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacggcatt
     7981 tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt
     8041 catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag
     8101 caatatatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt
     8161 ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata
     8221 tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt
     8281 cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc
     8341 aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga
     8401 tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc
     8461 tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa
     8521 gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca
     8581 ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat
     8641 atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat
     8701 gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac
     8761 gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga
     8821 gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat
     8881 ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc tcatgttttt
     8941 accctctttt ctttttgtat ttagataact cccttatctt gcagaactgg ggtgattttt
     9001 tcttcaattt cattttttga aacaatattt ttcaaataga tcatcgccgt tttaggatca
     9061 actgcgaact tttcgaactg cgaacgaaaa ttttctgctg tgatgatcac atctggtttc
     9121 atggaaactg cggaagaaat atcacaatgg tccagtttgg cttctactcc taatttgttc
     9181 aaaacatcct cagcagacat ctgtgctgca aaactacttc ctaaacctgc gccgcatacg
     9241 aataaaatgt tcaatttgtt catctttgtt ctcccccgta tcatttatta tttctcaact
     9301 actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc attcaatcaa tcgatccaac
     9361 ttctcttcgt tgttgatcaa ttcaattagt tctttcatga tgttcagatg ggagtaggag
     9421 tctactgctg ccaaacaaaa aataatcttt actggatcca tttcaggatt accgaaatcg
     9481 attggttgct caatcgtagc tacgctgact cctaatttat tgaccccatc ttctggtcgg
     9541 gcatgggcta gtgccatgtg tttgccaatc acgatatacg gtccgtaatc attcactgca
     9601 cgaatcatcg cttcgacata gcgctcttcg acaatgtttt ctttaaccaa tggtctagca
     9661 gctagttcaa tcgattcctc ccaagtagtg gctttttctt tgatcatgat attgctgtcc
     9721 gtcaaaatat cttttaacat aggctggatc tcccttttgt tgatttctaa attgtttgtt
     9781 tcgaataatt tttctaactt gccatagatg attcgatcta ctcggccacc actttctttc
     9841 atgacatcta ttatttgagt gaataactcc gtcgattcat gactgttagg ctctaaccgc
     9901 tgtagttctt catttctttt caaaaagtct tctataacca agatattcgt ctcagttaaa
     9961 atcggatcga caactaaaag cggcttattt cgataattca attgaaaggt gctgaaaacc
    10021 agattgacat ccaatttatc tactaattcg atttcttttg aacttaaaac tgcagtaatc
    10081 tcgatctgag gaaacttttc tttcagattc tctgctaaca gatttccagt agccatccca
    10141 tggttacaga caacgactga tttataaaca taattcaagt ctcgcttgat tacactggcg
    10201 atcgtcgaaa agtggatgac aagaaaagct atctcgtcct ccagtatctt gccaccaatt
    10261 acttcttcga tcgtaggaac aaaatgagtg actgcgcggt agattgggaa ataatttgtt
    10321 cggatgtttt ctttcaatgg attcatgact tggatatgat tcttgattct tacaatcagt
    10381 gcagccatgt gcttatagag attttcgaat aaggcttctt cctttagatg gaaaggtacg
    10441 tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta gtaattgagc ttgtacccac
    10501 tcgattgaat tggagatatc tctagcattg aacgtctcaa tagtgaaacg aagataattt
    10561 ctttctactt gcgggacatt gtccaattga aattcctcga tcatgtggtc aataaatgca
    10621 gaaaagctac tgtcttttat ttctgtattt ttccaattaa tggcagagat aaattcatgt
    10681 ttcctgattc gctggatcca gatcaatgta aaaagcagga tttgtttttt ataaatatca
    10741 tcttcttgtt tcaacaactt attggaatag atctcctgta tttttttgat ttcctccaaa
    10801 ggaaaataat ggagaaagat tttttgagcc ggtgttaatt gtgcatcttg attcataaag
    10861 aagtcggtca ttcccagatt ctgattgata aaatcataga tcatcgtacg gatagatcgt
    10921 tctgaacctt tataaaccag tccttgcttt ccataactga cgatctctat cccataactg
    10981 accagtgttg ctcgtaaccg tctcatatct tcatctaatg tacttttaga gatttgaaat
    11041 tcttcttctt ttttgtttag atggatcggg attcggctgt aagcaacggc taaaactaga
    11101 tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca catcttttat ttcagtttct
    11161 aatcgagctt gcttttcctt ttccagcttc aacaaaaaac ctttgttgcg aattgtacgg
    11221 atctccggaa gctttttact gctcaaccag atattcgctt caagaacttc ctgacgaatc
    11281 gtccgctgac tgacattgaa ctcacttgct agatgttttg cttttactgg atattgggct
    11341 gtcatgattc tgtgaagtat ttcaattgtt cttgttttca tagaaatgat ctccttctga
    11401 tttcaagtat atggttccgc ttacataata aaaagtagat atttctgcca cttgaagtgg
    11461 aaagaaagta agcaaagcaa acaaaaaata agccatatgc tttttcatct cctcaacgaa
    11521 aaagacatac gcctattatt acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa
    11581 aaagctgtcc tgcatcaagt aataagaaca actaaactaa aatagtgctt cctatttttt
    11641 gtttggttgt tcttattatc gcagatgcaa gcttttaaac accatttagt ggagaagctg
    11701 tgacagagat gttcagcctt gagcgataag aagcgatttt gaaaaatcgc ttcccatatt
    11761 ttacaaaatc gtgccttatt gccgataggc tatctctgga acaccgttta ttcagaaaaa
    11821 ggagctgcga caaggctttt gtcacaactc cgttcataaa tatttcctgt caatccagca
    11881 taatcggttg aataaaatcg gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc
    11941 atatctgaga aaaatactgt cgttgtgtct tacttcctga gatcaatcgc ccatcttttc
    12001 gatcgactga ataatcacag catctgaaat caattcggat ggcaaaacta ttttcgtgga
    12061 tctgtcgata atttcctact tcctgaatag ttattttttt agtatgattt cttacaccgt
    12121 ctgctttgtt tttttgcaga actttctgat gttcggcaaa cagcttgtct gtataacagc
    12181 ttctggcttt ttcaggagtt tcttcttccc aagcggattg gatcttcatg aatatctttt
    12241 ggatttccaa aaactgtgct ttcttttctt tagccgttcc tgacatgcgg gacaatatct
    12301 ccgatttcag ccttttcttt tgtcttttat tcttgacgat agaaaaagca gaaacccctc
    12361 ctactgacaa aacagctaat gcaagaaacc ccgtttgctc ataactagaa ggacctgtac
    12421 ttggaccata atacccataa gaatctctgc cgtaagaacg actgccaaaa taactgcccc
    12481 catatgaacg accgccaaat gaacctctgc ccgaacttcg accgctagat gtacctccac
    12541 catgaccacc cgcgacagcg aatccttggg tcggcagagc aaacaaacat aggcccacta
    12601 acaaaatgat tgctaaataa ttgatttttt cattgtcttc ctcgttatct tttattacaa
    12661 aaaactattt ttatcatagc cttgctaaaa ggaagagtca ataaccagat gcataaactg
    12721 tttttttagc aattttgtct tatttagtca ttgagtaaca tcggacagat gtatgtgtcg
    12781 gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag atctgcgctt
    12841 cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa tggacgattt
    12901 ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa cgttttctga
    12961 tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa caacatatgc
    13021 atttgtttct gtacatatga atcattatct agctccttag ctgcttggat tgcaagcctg
    13081 atatgcggat accctaatat cgcttcttca tatgcaccat ttacgccata tttttgcttc
    13141 accctatgac catgagtggt ttttttatga cgatattttg tatcaggaaa agcagcaagc
    13201 ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact gattttttgt
    13261 gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt attgatgcca
    13321 tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat tctttctctt
    13381 aactcctgat caatagcata atgccatgct atttgagcca tttcttcaaa aaaaggagtt
    13441 agcgtcttag cagacttatc caaaatgcgc cgaccttctt ttacggcttt tgcgatcgta
    13501 tggaaattat catctcctaa aaccatatca gctgcctgtt tgcttacttc actacctttg
    13561 attcccatag cgattcctat atcggctctt ttcaaagcag gtgcatcatt tacgccgtct
    13621 ccggtcattc cgacgatttc tccatttttc tgcaaagctt tcacgattcg cagtttgtgt
    13681 tctggtgtcg ttcgagcaaa gacgtcgact tttggtactt gtcgaatcaa ctcttcttca
    13741 gacataagat ccaaatcgat cccttctaaa actttagcag tatgttttaa gccaatttgt
    13801 tccgcgatcg cttttgctgt atctttgtga tcccccgtaa tcattttcac tgagattcct
    13861 gcttgttggc attcctttac agctttgact gcactttctt ttggcggatc aatcaaccca
    13921 gcaatccctg caaaagttaa ttgggttaaa gactcatgcg tgagttcctg tttcgaatct
    13981 acttttttgt aagcaaatcc taaaacacgc tgaccttttt tcgcaaacgc tgtggctgtt
    14041 tgaccccatg cttcttattc tgaagaagaa agagttgatt tagccaataa tacttctggc
    14101 gcacctttga cgtaaatcac ccctcctttt tcctccgcat gcatcgttgc catatatttg
    14161 tattcagagc tgaacggaat ttttgcttct atttttttca aaggcagatc ggctgtttcg
    14221 gtgtggcgaa tcagtgcaag ttctgtcgga ttgccactta aattttctgt tttctgttct
    14281 tctttcgtct ttaattcctg acaatttttc ataataaaca gtacgcgagt ttcttccttt
    14341 gctgctacat ccacaactgt catttcattt tttgtcaatg tccctgtctt atctgaacag
    14401 atcactgtca tagaacctaa tgtttctaca gaaggcagtc ctttgatgat cgccttttct
    14461 tttgccattt catgtactcc gacagatagg atcatggtca acacagctgg caatccttcg
    14521 ggtaccattg acaccaccaa agcgatcata gctgaaagaa ggatatgcca atccatgcca
    14581 tagcggaatg ttgtaaaaac aactaagaac aagatcaaac aaagaatgcc ttgaaaaatt
    14641 tgtttgttca atcggttgat cttcttgatc agaggcgtcg tttgttgttc gaccgattgc
    14701 agtgcttgat tgattttacc gatttctgtc gtgtttcctg tttctacaac gacaccgact
    14761 gcagaaccag attgtacaag cgttcctgaa aaagccatat ttttttggtc accgattgat
    14821 tcttctgctg ccaaaacgga atgatttttt tcagatgctt ctgattctcc cgtcaaaatc
    14881 gattcttcta ctagtagatc atgtacttcg atcaaccgaa catctgctgg aacgacgtct
    14941 ccggcacgta agacaatgac atctcctttt actagttctt tcgcagggat tttttcttgc
    15001 atcccgttcg atagtactat cgcttcttgt cccatcagct gcttcaaacc attcaacgat
    15061 tcttctgctt ttctttcttg ccaataacta acgagtccgt tgacgatcac tacgaataaa
    15121 ataatacttc cttcgatgta ttcgctcgaa gcaaatttca aaaaagaggc aatgatcaac
    15181 acgatcatca gcagatcagt aaagtgtttt gctatttttt tccatcgttt tgtctgctgt
    15241 cttgcttcta tctcattata tccttgatca gtcaggcgtt tctgccgttc ttcttgcgac
    15301 aagccttctg ttgatgtttg atagtttttc aatacatctt ctaccgtcaa tctgtaccag
    15361 ttcatcctat cccctcctgt ttaattgata ctgttattat acgcagggaa gttttttcag
    15421 gcgtatcgat tatttcaggg gggtctgaaa taattcgttt tgcagaatgt atctgggcct
    15481 aactgatatt tctattgctt attcttttat atacaaaatt taagaataca aaccagataa
    15541 ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa caagctgaaa gcaattgaag
    15601 taaaaaacac caacaaagca agatctttat atctttgtgt agtgttccgt ttcttttctt
    15661 taataaattg aacatgtgca cacaagatcg aagaccacac aaatagaaaa caactcgttg
    15721 tcatgcctgc caaaagctga aaaaacaatc gactatcagc tacaaaaaaa tgtagacaaa
    15781 gaccgacaaa aaataacatc cctgtaatta aaataccgtg taaaggtacg cctttcttag
    15841 aaagcgcaca aaaccaatgg ggcaaacttt tctcttttgc tgtttgatat aaaatcctgc
    15901 tagtactgta gactgcacta tttgctgagg acaaagaagc aaccaaaatc attagattga
    15961 caattgctgc tccgctttgg caatggatca cttgcagcat agtcactaaa ggactttctt
    16021 ttaaatctaa acaatgccat ggaacaacaa ataaaatcga caaaatcgtt cctatataga
    16081 aaaagaggat acgccaaggc agacagttga ttgcattttt caaattcttt tgtgggtcac
    16141 tcgtctcacc aactgttaat ccgatcattt caattccagc aaaactgaat aaaaccatag
    16201 gaaatgaagc aatcattcca gcaaagccaa acgggactaa ttcattgagc tgtccaaaat
    16261 ttgctttgat tccaatagac tgggtatgaa aatctgagac actcaatcct atacccaata
    16321 agataaaaag gaaaatcccc aagatcttga tgattgagaa aaaagattcc aattcaccaa
    16381 accacttgac tgcaacaagg ttgactgcca ttaagatcat tagtgcgcta cctccgctta
    16441 tcaaagctgg tacatgtaca aaccagaaat ggatatacaa tcctattgct gttaattccg
    16501 caagtccagc tccaagccag caaagccaat aagtccaatt gacaaaaaat gcccactttt
    16561 tgcctaaata atagtcaacg aaatcactga tagagttaaa tgtactattt gctaaaaata
    16621 aatcacccat gacccgcatc aaaccataaa tagccaaacc gcatatcaaa taaacgagaa
    16681 taacagctgg ccctgctgat gcaattgcct ttgctgaacc taaaaataat cctgttccaa
    16741 ttgcgccacc taatgcgatt aactggatat ttcgattagt tagagtccgt ttcattgccg
    16801 gtttttccat caagttcacc tttttctttt tcaagcagca tggcgacttc ctgttcccaa
    16861 gaaggtttct ttattttttc aatacagagt ggtactacaa acaaaatgac actgatggct
    16921 gtgactgcaa ttagattgat ccatgtccca gctttcatcg tcagtccgac agaaaatccg
    16981 attgccgcaa ataaaacaat aacgacagtc caaactccaa gattgctctt tttctctttt
    17041 tttccaattc taaatggtcg attcaacttt gcatgtgtat aacgcaatct caaaactgcg
    17101 atccccatga tgacatagac gatacagtag ataacagtag ttgaatttac gagcatcaaa
    17161 aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg caaagattga gataatgaca
    17221 gactgagtca aaataacgga tttggatacg tcaaattgat tcgtcttcca aaaattgaat
    17281 tttggtggat acgcaccttt tctagcactt tctgtcactg tctttgctgg tcctgaggcc
    17341 caagcagata attggactgc tacaccaatg aagacaagta aactaaacag attcacaata
    17401 agatgtggca aaccaagaat ttggcaataa atcgaaatag attgcgcgat attattcaat
    17461 tccatttgtc cttttggtac gacatttgct acaactaaag cattcaaaat attgacgaaa
    17521 aatataaaaa ttaacgcggc aaatactccg cgaatatacg ttttaactgg atttttcaac
    17581 cgagtgatat acacagagga catttcgata ccagtaaaaa taaacatgat cggcgcaaaa
    17641 taaacaaatg aggatgcagt cgtagtatct ggtattaatt tgtcccatga aaaactgcct
    17701 aatgttccgg ttggtacgat cccaactttg atccacgcag caaaccctaa taagagcatc
    17761 atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt ttccgccgat ttttgccata
    17821 tcaaatttca aattcaaaaa agtgatgatc caataaacga ctaaaatgac aactaatgta
    17881 aatttgctgt tcaaaccaag cggcacatta tcgataatat taccaaacaa cggagcaagt
    17941 gctgaagcga ccatcaccat cccaggaaac atttggaccc ataacagcca tgagacaacg
    18001 aacccccatt tttttcctag agcattcgat acccaaagtt ctggaccacc tttttgggga
    18061 aacattccgg caaattcacc agagatcaat gagattggaa aagcgtacaa taatactgca
    18121 accagcatgt aaaaaatcat cgtccagctc gttgctgcta catctggaat atttcgtaca
    18181 cttgctacaa gggcgcaggt cattccgata aaggtcatgg tcgtgatttg ctttgatgaa
    18241 ccagtatctt tcatctttat tccttctttc aatagcgaca tatgtttttg ttttctttta
    18301 tttttgttca agaacagaaa tgacaaggtt tcatcgattc agtacaaaac ccagtatcac
    18361 cgaaacatcc attgaacgat cttagtgaac tgggtttgat acatgaaacg aacgatccaa
    18421 cttatagaac gttttataaa aaagaatatc ttggcgagga atatatgaaa aacaggggga
    18481 aatacaattg ttttgatgaa gcaaataatt gtctgttctc catatgtctc ttgttattct
    18541 ttcacacaaa aatcagttct attggatgat ttcgtttagt ttcttttcca ttgctttggc
    18601 gatttctttt gtataaaaat tgaaaattcg atcatcattc aaataaggag tcatgatagc
    18661 cgcacgtagc agagtgattt tctgttcttt tttccattct tctttcgtta gacccattct
    18721 ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt gtaaatgtag tatgagaagt
    18781 aataaaccgt tcaccataaa catcgccatc catataagaa gaccgatcga acattttttc
    18841 atttaattca ttgatagcgt tcaaatctgt acagccttgt tctttgaata cccaattgac
    18901 catattaaaa tcaggatgat tcaatggata gacttcgatt gtttttcctt taacagtaaa
    18961 agtcagatga tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa
    19021 ttgaccatat ccggtcacat tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc
    19081 ggttgctcca gcttttgatc cttccaaaat ataagctcct aacatatctg gagcctttac
    19141 tgatttttca aacacatatg gcgcgaaata agaaatgata tttctcatat ttttatgttt
    19201 gattacgatt cctccagcag cataagggac atagcccatc ttatgaggat ctatcgtgac
    19261 agaatccgct tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca
    19321 gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag gaagcatacg gcacaaattc
    19381 tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca tatgcggcat caacatgtaa
    19441 atagaaatag atcccttcat cttttaacct ttctcttaat tggacgattt tatctacact
    19501 gtctacttga ccttcttctg tcgttcctac gacagcaacc acacctagaa ttgggatttt
    19561 ttgttcagct aattcacgaa ttgttttttc caaagcatta atatccattc gataatcctc
    19621 ttgaactggg atagcaacca tctgatctaa gcctactccg caaatatcta acgccttcat
    19681 ccaagaatag tgttttgttt gaggcaccag ccatttccct aatctttgga tattttttcc
    19741 gctacgggaa gaagctgctt tgacctcatc catttcttcg tccgtaaagc tttcggtcat
    19801 ttccaaaatt tcttcaacag ataaattcaa aagttcccac tcagacattt ttttcacttg
    19861 ttcaggcagc acttccttga cagctaaagg aatcgatttg atgcagcggg cgtaccataa
    19921 tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt
    19981 gaataatgaa gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg ccatagaaga
    20041 ttccaatgca acattatttg ggttccacag catcgcatag ttgtaagcta ataaagcagg
    20101 catcaatgtt tcagcattca tttgtcccca gtaacggcct gctgaatgcc atgggatcga
    20161 tcctgcgcgt aatcgctgac tgacttcatc caagacctct atcatatggt cgcgggtggc
    20221 tagataatct ggagtgagct tatctccttc gctaattgca ggcatatctg aaggaatata
    20281 attttctctc catccaagat gttcatctac catcttgttc aacaacattt tataaactgg
    20341 tccattttca gctttgtcgc ctataaagac ggccttgata tccatatctt tcatttttct
    20401 tccctccata ttttgttggt caatgtctcg cagatacatg tgtttatctg taacacctct
    20461 tcactatagc gccaaaaatg agaaattttt ttttataaca tcaaatccac gtagattttt
    20521 ac
//
LOCUS       DASHWH010000049        20363 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.052, whole genome shotgun sequence.
ACCESSION   DASHWH010000049 DASHWH010000000
VERSION     DASHWH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.052"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            292..1431
                     /locus_tag="U2R41_001874"
     CDS             292..1431
                     /locus_tag="U2R41_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEM8889272.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="U2R41_001875"
     CDS             complement(1498..2073)
                     /locus_tag="U2R41_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8889273.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="U2R41_001876"
     CDS             complement(2078..3052)
                     /locus_tag="U2R41_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEM8889274.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="U2R41_001877"
     CDS             3335..4303
                     /locus_tag="U2R41_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8889275.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="U2R41_001878"
     CDS             4416..4694
                     /locus_tag="U2R41_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM8889276.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="U2R41_001879"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="U2R41_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEM8889277.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="U2R41_001880"
     CDS             5151..5906
                     /locus_tag="U2R41_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8889278.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="U2R41_001881"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="U2R41_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEM8889279.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="U2R41_001882"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="U2R41_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEM8889280.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="U2R41_001883"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="U2R41_001883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEM8889281.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="U2R41_001884"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="U2R41_001884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEM8889282.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="U2R41_001885"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="U2R41_001885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEM8889283.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="U2R41_001886"
     CDS             10643..11185
                     /locus_tag="U2R41_001886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEM8889284.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="U2R41_001887"
     CDS             11208..12764
                     /locus_tag="U2R41_001887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEM8889285.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="U2R41_001888"
     CDS             12778..13680
                     /locus_tag="U2R41_001888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEM8889286.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="U2R41_001889"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="U2R41_001889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEM8889287.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="U2R41_001890"
     CDS             15123..15545
                     /locus_tag="U2R41_001890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEM8889288.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="U2R41_001891"
     CDS             16014..16244
                     /locus_tag="U2R41_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEM8889289.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="U2R41_001892"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="U2R41_001892"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM8889290.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="U2R41_001893"
     CDS             17655..17831
                     /locus_tag="U2R41_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM8889291.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="U2R41_001894"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="U2R41_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEM8889292.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="U2R41_001895"
     CDS             18326..18709
                     /locus_tag="U2R41_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889293.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="U2R41_001896"
     CDS             18878..19762
                     /locus_tag="U2R41_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8889294.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="U2R41_001897"
     CDS             19876..20112
                     /locus_tag="U2R41_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEM8889295.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="U2R41_001898"
     tRNA            20200..20280
                     /locus_tag="U2R41_001898"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="U2R41_001899"
     tRNA            20287..20358
                     /locus_tag="U2R41_001899"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DASHWH010000050        19413 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.053, whole genome shotgun sequence.
ACCESSION   DASHWH010000050 DASHWH010000000
VERSION     DASHWH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.053"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="U2R41_001900"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="U2R41_001900"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEM8889296.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="U2R41_001901"
     CDS             complement(1174..1869)
                     /locus_tag="U2R41_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEM8889297.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="U2R41_001902"
     CDS             complement(1882..2598)
                     /locus_tag="U2R41_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEM8889298.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="U2R41_001903"
     CDS             2928..3533
                     /locus_tag="U2R41_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEM8889299.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="U2R41_001904"
     CDS             3858..4181
                     /locus_tag="U2R41_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEM8889300.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="U2R41_001905"
     CDS             4363..5733
                     /locus_tag="U2R41_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM8889301.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="U2R41_001906"
     CDS             complement(5863..7164)
                     /locus_tag="U2R41_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM8889302.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9352)
                     /locus_tag="U2R41_001907"
     CDS             complement(7394..9352)
                     /locus_tag="U2R41_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEM8889303.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="U2R41_001908"
     CDS             complement(9529..9795)
                     /locus_tag="U2R41_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8889304.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="U2R41_001909"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="U2R41_001909"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEM8889305.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="U2R41_001910"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="U2R41_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEM8889306.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="U2R41_001911"
     CDS             13139..13702
                     /locus_tag="U2R41_001911"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM8889307.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="U2R41_001912"
     CDS             complement(13955..14476)
                     /locus_tag="U2R41_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEM8889308.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="U2R41_001913"
     CDS             complement(14479..15321)
                     /locus_tag="U2R41_001913"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEM8889309.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="U2R41_001914"
     CDS             complement(15314..16039)
                     /locus_tag="U2R41_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEM8889310.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="U2R41_001915"
     CDS             16215..16511
                     /locus_tag="U2R41_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889311.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="U2R41_001916"
     CDS             17079..18671
                     /locus_tag="U2R41_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8889312.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..>19413
                     /locus_tag="U2R41_001917"
     CDS             18684..>19413
                     /locus_tag="U2R41_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8889313.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRI"
BASE COUNT         5550 a         3989 c         3682 g         6192 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttt
//
LOCUS       DASHWH010000051        19308 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.054, whole genome shotgun sequence.
ACCESSION   DASHWH010000051 DASHWH010000000
VERSION     DASHWH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.054"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            968..2218
                     /locus_tag="U2R41_001918"
     CDS             968..2218
                     /locus_tag="U2R41_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEM8889314.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="U2R41_001919"
     CDS             2237..3247
                     /locus_tag="U2R41_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEM8889315.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="U2R41_001920"
     CDS             3257..3910
                     /locus_tag="U2R41_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889316.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="U2R41_001921"
     CDS             3929..4927
                     /locus_tag="U2R41_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEM8889317.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="U2R41_001922"
     CDS             5003..5764
                     /locus_tag="U2R41_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEM8889318.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="U2R41_001923"
     CDS             5782..6888
                     /locus_tag="U2R41_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8889319.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="U2R41_001924"
     CDS             6878..7111
                     /locus_tag="U2R41_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889320.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="U2R41_001925"
     CDS             7310..7765
                     /locus_tag="U2R41_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEM8889321.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="U2R41_001926"
     CDS             7779..8552
                     /locus_tag="U2R41_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8889322.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="U2R41_001927"
     CDS             8539..9669
                     /locus_tag="U2R41_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8889323.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="U2R41_001928"
     CDS             9700..10935
                     /locus_tag="U2R41_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889324.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="U2R41_001929"
     CDS             10960..12069
                     /locus_tag="U2R41_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889325.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="U2R41_001930"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="U2R41_001930"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEM8889326.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="U2R41_001931"
     CDS             13480..15117
                     /locus_tag="U2R41_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEM8889327.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="U2R41_001932"
     CDS             15219..15614
                     /locus_tag="U2R41_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEM8889328.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="U2R41_001933"
     CDS             15632..16363
                     /locus_tag="U2R41_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8889329.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="U2R41_001934"
     CDS             16387..17838
                     /locus_tag="U2R41_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM8889330.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="U2R41_001935"
     CDS             17864..18475
                     /locus_tag="U2R41_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8889331.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="U2R41_001936"
     CDS             18657..19172
                     /locus_tag="U2R41_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEM8889332.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4217 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatatt
//
LOCUS       DASHWH010000052        18895 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.055, whole genome shotgun sequence.
ACCESSION   DASHWH010000052 DASHWH010000000
VERSION     DASHWH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.055"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..251)
                     /locus_tag="U2R41_001937"
     CDS             complement(21..251)
                     /locus_tag="U2R41_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889333.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="U2R41_001938"
     CDS             complement(687..1253)
                     /locus_tag="U2R41_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8889334.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="U2R41_001939"
     CDS             complement(1253..2209)
                     /locus_tag="U2R41_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEM8889335.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="U2R41_001940"
     CDS             complement(2209..2865)
                     /locus_tag="U2R41_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM8889336.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="U2R41_001941"
     CDS             3235..5253
                     /locus_tag="U2R41_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8889337.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="U2R41_001942"
     CDS             5680..8094
                     /locus_tag="U2R41_001942"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEM8889338.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="U2R41_001943"
     CDS             8442..9995
                     /locus_tag="U2R41_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM8889339.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="U2R41_001944"
     CDS             complement(10376..12160)
                     /locus_tag="U2R41_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889340.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="U2R41_001945"
     CDS             complement(12175..13896)
                     /locus_tag="U2R41_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889341.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="U2R41_001946"
     CDS             13996..14757
                     /locus_tag="U2R41_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8889342.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQFLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="U2R41_001947"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="U2R41_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="U2R41_001948"
     CDS             16664..>18895
                     /locus_tag="U2R41_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889343.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6204 a         3353 c         3786 g         5552 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtttctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DASHWH010000053        17586 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.056, whole genome shotgun sequence.
ACCESSION   DASHWH010000053 DASHWH010000000
VERSION     DASHWH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.056"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            363..731
                     /locus_tag="U2R41_001949"
     CDS             363..731
                     /locus_tag="U2R41_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEM8889344.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="U2R41_001950"
     CDS             911..1852
                     /locus_tag="U2R41_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEM8889345.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="U2R41_001951"
     CDS             1921..2652
                     /locus_tag="U2R41_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEM8889346.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="U2R41_001952"
     CDS             2796..4976
                     /locus_tag="U2R41_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEM8889347.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="U2R41_001953"
     CDS             4996..5478
                     /locus_tag="U2R41_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEM8889348.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="U2R41_001954"
     CDS             5494..6255
                     /locus_tag="U2R41_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM8889349.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="U2R41_001955"
     CDS             6386..7720
                     /locus_tag="U2R41_001955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEM8889350.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="U2R41_001956"
     CDS             8027..8512
                     /locus_tag="U2R41_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEM8889351.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="U2R41_001957"
     CDS             8505..9188
                     /locus_tag="U2R41_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEM8889352.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="U2R41_001958"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="U2R41_001958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEM8889353.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="U2R41_001959"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="U2R41_001959"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEM8889354.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="U2R41_001960"
     CDS             12804..13433
                     /locus_tag="U2R41_001960"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEM8889355.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="U2R41_001961"
     CDS             13485..13817
                     /locus_tag="U2R41_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM8889356.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="U2R41_001962"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="U2R41_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEM8889357.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="U2R41_001963"
     CDS             14643..16016
                     /locus_tag="U2R41_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM8889358.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="U2R41_001964"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="U2R41_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEM8889359.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="U2R41_001965"
     CDS             17071..17511
                     /locus_tag="U2R41_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM8889360.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5992 a         3067 c         3866 g         4661 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatggg
//
LOCUS       DASHWH010000054        17438 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.057, whole genome shotgun sequence.
ACCESSION   DASHWH010000054 DASHWH010000000
VERSION     DASHWH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.057"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            282..521
                     /locus_tag="U2R41_001966"
     CDS             282..521
                     /locus_tag="U2R41_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889361.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="U2R41_001967"
     CDS             complement(883..1143)
                     /locus_tag="U2R41_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889362.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="U2R41_001968"
     CDS             complement(1328..1825)
                     /locus_tag="U2R41_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEM8889363.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U2R41_001969"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U2R41_001969"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEM8889364.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U2R41_001970"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U2R41_001970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEM8889365.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="U2R41_001971"
     CDS             complement(4547..5209)
                     /locus_tag="U2R41_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8889366.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGSSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="U2R41_001972"
     CDS             complement(5219..6034)
                     /locus_tag="U2R41_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8889367.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="U2R41_001973"
     CDS             complement(6296..6739)
                     /locus_tag="U2R41_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEM8889368.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFGLIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="U2R41_001974"
     CDS             complement(6873..7211)
                     /locus_tag="U2R41_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8889369.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="U2R41_001975"
     CDS             complement(7199..7576)
                     /locus_tag="U2R41_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8889370.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="U2R41_001976"
     CDS             7800..8993
                     /locus_tag="U2R41_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8889371.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="U2R41_001977"
     CDS             complement(9159..9581)
                     /locus_tag="U2R41_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889372.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="U2R41_001978"
     CDS             9976..10170
                     /locus_tag="U2R41_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889373.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="U2R41_001979"
     CDS             10167..10661
                     /locus_tag="U2R41_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889374.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="U2R41_001980"
     CDS             complement(10823..11995)
                     /locus_tag="U2R41_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8889375.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="U2R41_001981"
     CDS             complement(12201..13016)
                     /locus_tag="U2R41_001981"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8889376.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="U2R41_001982"
     CDS             complement(13166..13612)
                     /locus_tag="U2R41_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM8889377.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="U2R41_001983"
     CDS             complement(13609..14613)
                     /locus_tag="U2R41_001983"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8889378.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="U2R41_001984"
     CDS             complement(14653..14982)
                     /locus_tag="U2R41_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM8889379.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="U2R41_001985"
     CDS             complement(15108..17435)
                     /locus_tag="U2R41_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8889380.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5036 a         3468 c         3041 g         5893 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa gaacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagccc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccc
//
LOCUS       DASHWH010000055        17104 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.058, whole genome shotgun sequence.
ACCESSION   DASHWH010000055 DASHWH010000000
VERSION     DASHWH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.058"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..1398
                     /locus_tag="U2R41_001986"
     CDS             7..1398
                     /locus_tag="U2R41_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM8889381.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="U2R41_001987"
     CDS             1414..2454
                     /locus_tag="U2R41_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8889382.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="U2R41_001988"
     CDS             2473..3558
                     /locus_tag="U2R41_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEM8889383.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="U2R41_001989"
     CDS             3591..4553
                     /locus_tag="U2R41_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM8889384.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="U2R41_001990"
     CDS             4546..5979
                     /locus_tag="U2R41_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEM8889385.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="U2R41_001991"
     CDS             5976..7046
                     /locus_tag="U2R41_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889386.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="U2R41_001992"
     CDS             7033..8454
                     /locus_tag="U2R41_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM8889387.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="U2R41_001993"
     CDS             8467..9474
                     /locus_tag="U2R41_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8889388.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="U2R41_001994"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="U2R41_001994"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEM8889389.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="U2R41_001995"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="U2R41_001995"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEM8889390.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="U2R41_001996"
     CDS             11608..12285
                     /locus_tag="U2R41_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM8889391.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="U2R41_001997"
     CDS             12275..13417
                     /locus_tag="U2R41_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEM8889392.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="U2R41_001998"
     CDS             13430..14407
                     /locus_tag="U2R41_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8889393.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="U2R41_001999"
     CDS             14407..15315
                     /locus_tag="U2R41_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEM8889394.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="U2R41_002000"
     CDS             15316..16068
                     /locus_tag="U2R41_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEM8889395.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="U2R41_002001"
     CDS             16073..17020
                     /locus_tag="U2R41_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEM8889396.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6163 a         2190 c         3088 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattacgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gata
//
LOCUS       DASHWH010000056        17005 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.059, whole genome shotgun sequence.
ACCESSION   DASHWH010000056 DASHWH010000000
VERSION     DASHWH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.059"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..124)
                     /locus_tag="U2R41_002002"
     CDS             complement(<1..124)
                     /locus_tag="U2R41_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEM8889397.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="U2R41_002003"
     CDS             complement(228..539)
                     /locus_tag="U2R41_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM8889398.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="U2R41_002004"
     CDS             complement(541..738)
                     /locus_tag="U2R41_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEM8889399.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="U2R41_002005"
     CDS             complement(1132..2859)
                     /locus_tag="U2R41_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEM8889400.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="U2R41_002006"
     CDS             complement(2852..4045)
                     /locus_tag="U2R41_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8889401.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="U2R41_002007"
     CDS             complement(4232..4876)
                     /locus_tag="U2R41_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8889402.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="U2R41_002008"
     CDS             complement(4970..5104)
                     /locus_tag="U2R41_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEM8889403.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U2R41_002009"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U2R41_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEM8889404.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="U2R41_002010"
     CDS             complement(7235..7708)
                     /locus_tag="U2R41_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEM8889405.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="U2R41_002011"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="U2R41_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEM8889406.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="U2R41_002012"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="U2R41_002012"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEM8889407.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="U2R41_002013"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="U2R41_002013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEM8889408.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="U2R41_002014"
     CDS             complement(11755..12894)
                     /locus_tag="U2R41_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8889409.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="U2R41_002015"
     CDS             complement(13203..13916)
                     /locus_tag="U2R41_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEM8889410.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U2R41_002016"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U2R41_002016"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEM8889411.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="U2R41_002017"
     CDS             15105..16637
                     /locus_tag="U2R41_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8889412.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="U2R41_002018"
     CDS             complement(16828..>17005)
                     /locus_tag="U2R41_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8889413.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DASHWH010000057        16823 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.060, whole genome shotgun sequence.
ACCESSION   DASHWH010000057 DASHWH010000000
VERSION     DASHWH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.060"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..405)
                     /gene="ssrA"
                     /locus_tag="U2R41_002019"
     tmRNA           complement(39..405)
                     /gene="ssrA"
                     /locus_tag="U2R41_002019"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(576..1349)
                     /locus_tag="U2R41_002020"
     CDS             complement(576..1349)
                     /locus_tag="U2R41_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8889414.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1493..1843
                     /locus_tag="U2R41_002021"
     CDS             1493..1843
                     /locus_tag="U2R41_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM8889415.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1824..2540
                     /locus_tag="U2R41_002022"
     CDS             1824..2540
                     /locus_tag="U2R41_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8889416.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2540..3988
                     /locus_tag="U2R41_002023"
     CDS             2540..3988
                     /locus_tag="U2R41_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8889417.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4058..4567)
                     /locus_tag="U2R41_002024"
     CDS             complement(4058..4567)
                     /locus_tag="U2R41_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889418.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4557..5282)
                     /locus_tag="U2R41_002025"
     CDS             complement(4557..5282)
                     /locus_tag="U2R41_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889419.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5540..6880)
                     /locus_tag="U2R41_002026"
     CDS             complement(5540..6880)
                     /locus_tag="U2R41_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8889420.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(7047..9167)
                     /locus_tag="U2R41_002027"
     CDS             complement(7047..9167)
                     /locus_tag="U2R41_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8889421.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9397..10575
                     /locus_tag="U2R41_002028"
     CDS             9397..10575
                     /locus_tag="U2R41_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEM8889422.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10591..11349
                     /gene="aroD"
                     /locus_tag="U2R41_002029"
     CDS             10591..11349
                     /gene="aroD"
                     /locus_tag="U2R41_002029"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEM8889423.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11372..11857
                     /gene="ybaK"
                     /locus_tag="U2R41_002030"
     CDS             11372..11857
                     /gene="ybaK"
                     /locus_tag="U2R41_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEM8889424.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11928..13181)
                     /locus_tag="U2R41_002031"
     CDS             complement(11928..13181)
                     /locus_tag="U2R41_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8889425.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13298..14647
                     /gene="gdhA"
                     /locus_tag="U2R41_002032"
     CDS             13298..14647
                     /gene="gdhA"
                     /locus_tag="U2R41_002032"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEM8889426.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14759..16105
                     /locus_tag="U2R41_002033"
     CDS             14759..16105
                     /locus_tag="U2R41_002033"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEM8889427.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16413..16799)
                     /locus_tag="U2R41_002034"
     CDS             complement(16413..16799)
                     /locus_tag="U2R41_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8889428.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5407 a         3035 c         3443 g         4938 t
ORIGIN      
        1 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
       61 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      121 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      181 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      241 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      301 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      361 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      421 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
      481 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
      541 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
      601 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
      661 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
      721 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
      781 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
      841 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
      901 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
      961 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1021 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1081 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1141 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1201 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1261 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1321 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1381 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1441 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     1501 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     1561 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     1621 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     1681 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     1741 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     1801 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     1861 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     1921 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     1981 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2041 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2101 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2161 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagtagttt
     2221 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2281 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2341 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2401 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     2461 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     2521 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     2581 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     2641 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     2701 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     2761 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     2821 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     2881 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     2941 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3001 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3061 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3121 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3181 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3241 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3301 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3361 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3421 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     3481 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     3541 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     3601 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     3661 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     3721 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     3781 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     3841 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     3901 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     3961 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4021 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4081 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4141 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4201 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4261 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4321 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4381 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4441 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     4501 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     4561 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     4621 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     4681 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     4741 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     4801 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     4861 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     4921 aaaaggaata atttggtttt agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg
     4981 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5041 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5101 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5161 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5221 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5281 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5341 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5401 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     5461 gcagcgacaa taattttggg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     5521 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     5581 tagtcagttg ggtagtgttc ttagttttct tgaaatattt tcctcgatat aaaaagattc
     5641 gttctcggta gttttgaaag tttctaaagc cataggcaat tcgttttagt actttgatat
     5701 ggttgttcaa acattctata gggccgttgg aatagggaac tcgcaacgct cgtttgattt
     5761 ctgttcgata tttcttgaag gtcgtgaaca ctggttgata gtgctctggc aactccttgt
     5821 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata gcggaaagaa
     5881 aaacctggta gacttcgtag cctcgcttta aggagtcatc ataatataat agccgatcga
     5941 ctatctccgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgaccat
     6001 tcaattccca cgcattcttt tgaagtagtc tccagtattt ctttaagtgc ttcgaccgac
     6061 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaag gcccgaccga
     6121 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     6181 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgtacttgt tcacggactt
     6241 ttctagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     6301 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacgtttt
     6361 tcaccgattt gaattcatcg aaacacacca cttctggtaa cggctggttc aaagaaacgg
     6421 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     6481 gtgcaatcaa agacatagaa ataggctcac ttaatagctc aagaatggct tgtttgactc
     6541 tgcgggcaat ggagcagcga cgatcgctaa ctgaatcttc tgctaaaaaa gtctttccac
     6601 agtgtttaca tttgaagcgc tgtttgttaa tccgcacaat ggtcttgtag ttactgacat
     6661 cattcaataa gatggatact tttttccagc cccatttaat gatcgtttgc ttattttgat
     6721 agtggcaatg gaagcattcc tctggttgat aggtcaatgt gccatcaaaa acaaaacatg
     6781 tttcgtcatt aatgcgctct ttgcgaaagg catcttcatg aaaggtaaga tttaggtcta
     6841 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat
     6901 ggttttcgtc gactttattc taaaggattt caaggtgtct gtttgcgttt attagaaaaa
     6961 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cctaattttg
     7021 tcgctgctcc ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt
     7081 tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag
     7141 acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc
     7201 ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga
     7261 agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt
     7321 gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta
     7381 ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa
     7441 gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt
     7501 gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg
     7561 tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt
     7621 tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt
     7681 ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca
     7741 tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg
     7801 gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct
     7861 tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa
     7921 aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc
     7981 aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa
     8041 tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg
     8101 aataccgcac taatgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta
     8161 ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc
     8221 atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa
     8281 ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga
     8341 ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata
     8401 taaatcacat tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt
     8461 tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg
     8521 tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg
     8581 accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata
     8641 aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct
     8701 cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata
     8761 aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga
     8821 ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct
     8881 cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc
     8941 gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta
     9001 gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg
     9061 aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa
     9121 aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt
     9181 tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca
     9241 tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat
     9301 taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt
     9361 tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga
     9421 gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc
     9481 caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa
     9541 ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca
     9601 tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt
     9661 ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc
     9721 ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta
     9781 tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga
     9841 ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga
     9901 aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta
     9961 aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca
    10021 gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct
    10081 gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt
    10141 ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc
    10201 attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac
    10261 atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca
    10321 aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt
    10381 attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag
    10441 gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat
    10501 ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt
    10561 aaaaataacc attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga
    10621 agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt
    10681 gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat
    10741 cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt
    10801 gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata
    10861 tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg
    10921 gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag
    10981 tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct
    11041 gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca
    11101 gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat
    11161 gcttttttct ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt
    11221 ctggttggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact
    11281 tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa
    11341 ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa
    11401 tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga
    11461 cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat
    11521 atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg
    11581 agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat
    11641 gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat
    11701 aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat
    11761 cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc
    11821 attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag
    11881 gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc
    11941 ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca
    12001 gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc
    12061 tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc
    12121 tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct
    12181 tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga
    12241 aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg
    12301 gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa
    12361 caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt
    12421 gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta
    12481 tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa
    12541 agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc
    12601 gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta
    12661 gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac
    12721 tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact
    12781 cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata
    12841 atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt
    12901 acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta
    12961 tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc
    13021 gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt
    13081 tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt
    13141 gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag
    13201 tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag
    13261 ctaaaatgag ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt
    13321 tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc
    13381 aatcgatgaa ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga
    13441 aaagaatatc ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg
    13501 gcaagatgat gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc
    13561 tatcggccct tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa
    13621 atttttggct tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg
    13681 aaaaggcgga agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg
    13741 tcaaagtttc atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg
    13801 agatatcggt gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa
    13861 aaaatatgat actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg
    13921 tacggaagct actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa
    13981 agattcattt aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc
    14041 aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta
    14101 catctatgat ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag
    14161 acaacgtttg acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc
    14221 tgtttggaca ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat
    14281 agacgaaagc ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc
    14341 taatatgcca tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg
    14401 tccaggaaaa gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa
    14461 cgtccagcga ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga
    14521 aacgatttac catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct
    14581 tcttggagca aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt
    14641 agtttgaaaa atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa
    14701 ataataaaaa aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat
    14761 ggcacacatt cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg
    14821 gtacatgcaa agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg
    14881 aagcgacttc cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg
    14941 tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat
    15001 cggtggttct tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa
    15061 tttattagat agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag
    15121 ctcaacatat attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt
    15181 tatttcaaaa tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct
    15241 tcttgttaag aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa
    15301 agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc
    15361 tgatgacatc ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt
    15421 tagtggtgca gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc
    15481 aagcgacaaa ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta
    15541 ccgtaaaggc aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc
    15601 agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc
    15661 ttcaagcgca aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg
    15721 tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc
    15781 tgaacaagca gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt
    15841 aaatacaaaa gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt
    15901 gctagtcaaa attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga
    15961 aattgctgta ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt
    16021 agaagcttac aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc
    16081 taaagaattg aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg
    16141 atagaaacat gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg
    16201 catcgtttat tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat
    16261 agaaatcgat tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct
    16321 gacttcgaag gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca
    16381 cttcgagtgc ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca
    16441 gatgagggta ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct
    16501 ttcaactgca agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta
    16561 atgacatcga aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat
    16621 ttaaaacctc ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt
    16681 ggagctttga ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa
    16741 cctttccatc ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg
    16801 aaagatctcc ttttttattt tta
//
LOCUS       DASHWH010000058        16612 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.061, whole genome shotgun sequence.
ACCESSION   DASHWH010000058 DASHWH010000000
VERSION     DASHWH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.061"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(396..1091)
                     /locus_tag="U2R41_002035"
     CDS             complement(396..1091)
                     /locus_tag="U2R41_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8889429.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            1245..1523
                     /locus_tag="U2R41_002036"
     CDS             1245..1523
                     /locus_tag="U2R41_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889430.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1542..2750
                     /locus_tag="U2R41_002037"
     CDS             1542..2750
                     /locus_tag="U2R41_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8889431.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2898..3263)
                     /locus_tag="U2R41_002038"
     CDS             complement(2898..3263)
                     /locus_tag="U2R41_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEM8889432.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3410..4184)
                     /locus_tag="U2R41_002039"
                     /pseudo
     CDS             complement(3410..4184)
                     /locus_tag="U2R41_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(4358..5218)
                     /locus_tag="U2R41_002040"
     CDS             complement(4358..5218)
                     /locus_tag="U2R41_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEM8889433.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5218..6636)
                     /locus_tag="U2R41_002041"
     CDS             complement(5218..6636)
                     /locus_tag="U2R41_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8889434.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6693..7142)
                     /locus_tag="U2R41_002042"
     CDS             complement(6693..7142)
                     /locus_tag="U2R41_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8889435.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7587..7850)
                     /locus_tag="U2R41_002043"
     CDS             complement(7587..7850)
                     /locus_tag="U2R41_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEM8889436.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7949..9244)
                     /gene="rho"
                     /locus_tag="U2R41_002044"
     CDS             complement(7949..9244)
                     /gene="rho"
                     /locus_tag="U2R41_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEM8889437.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9241..10512)
                     /locus_tag="U2R41_002045"
     CDS             complement(9241..10512)
                     /locus_tag="U2R41_002045"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM8889438.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10677..11546)
                     /locus_tag="U2R41_002046"
     CDS             complement(10677..11546)
                     /locus_tag="U2R41_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEM8889439.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11838..13448)
                     /locus_tag="U2R41_002047"
     CDS             complement(11838..13448)
                     /locus_tag="U2R41_002047"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEM8889440.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="U2R41_002048"
     CDS             complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="U2R41_002048"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEM8889441.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(16009..16401)
                     /locus_tag="U2R41_002049"
     CDS             complement(16009..16401)
                     /locus_tag="U2R41_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8889442.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4741 a         3526 c         2825 g         5520 t
ORIGIN      
        1 gtgtcacagc cccttttgtt gcaaataaac ggcgttcaaa agcttacatc tacggtgata
       61 agtccagcca agttaaaaac ataagagact gtttttactt ggctcttctt attacttgat
      121 gcagaacagc tttttcacaa cctcttggta aacggtgttc tcctaccaac tcctcggtca
      181 caagtcacaa tattcatcaa acatgaaaag ctgttttttg aaattgttcc ttgtgactca
      241 gagttaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc
      361 aggtcaaaac gctgatttca caacctcttt gttcatcaaa agataatatt gtcttctaac
      421 aagtccatat tcaaaataac gatatgatgg tctttaacgg tgataatacc atcttttttc
      481 agttgtttta aaattctgtt tacactgcta gcagttgaga tgccacaaaa tttggcgatt
      541 tcttcattcg tgatcgaaaa atcgatcagc atcccttctt ctgtccgcac accgaataaa
      601 tcatataatt cgtaaagttg cgtacaaaca gctcccagct ttccgtttaa tagcatttgc
      661 tgcatttttt tcatcgaata aagcagacga tagcggtaat actcttttac atagtgttgt
      721 agctttgggt cttggttgat attttgccaa aagcgcacac gattgattcg ataaaactct
      781 gcatattctg attcaactcg aatattatac ggcgaatcga tgaattgtga atattcgtct
      841 tttaaaagag agacgatttc aagatctttg atatagcgaa ggttaaattc tctcccatca
      901 tttgaaataa cacttgcttt taccgttccc tctttcaaga tatacgtatg ggaatcttcc
      961 atcccttcga atgtcaaata tttatgatat cctttgcgta taatcggaaa atcattttta
     1021 tccaaatatt cctttaagac tctactatcc aatgatgctc ccccatccaa actttcatct
     1081 aaaaagatca tacagtactt taaaactttt taaagtccaa aggtatttta ctaatgatat
     1141 taacatttgt taagtacctt tttccatcac ttatgcgatt tttttccttt tttttattcg
     1201 ttatactgca gaagtaaaat tttttaagaa tagagggaaa aaacatgaat tactggatga
     1261 acacaatcat taatcgatta gaaacagcat atcaaacaag atttgatatg aaagcttctt
     1321 tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt gatcaaagca gtggatgaga
     1381 atccaacaaa cgaatgcgaa gaatttttaa atctgtttat gtctacaaga gatttattta
     1441 ttcgccaact tgttgatcgc tatccatcca attatcacga tgtagaagtt caaatacaaa
     1501 aattaaaagc atattcagct taaggggaat gtataacata catggaaaag aaattattta
     1561 ataaatattt tattggaatc acattgatca attttattgt ctacctgatt tattatctgt
     1621 tgatggtcat catggcagtc attgcgcaag acaccttaca cgccagcttg agccaagcag
     1681 gttttgcttc tgggatctat atcatcggaa cattgctggc acgcttattc atggggaaac
     1741 agttagaact atttggtcgg aagtttacct taagaggcgg cgcgatcttc tatctccttt
     1801 caacattagc atatctttat acgccaacta tcggggcatt gtatttagtt cgattcttga
     1861 atggattcgg ctacggtaca gtttctacag ctacaaatgc tatcgttact gcatatatcc
     1921 ctgcatctaa aaaaggggaa ggcatcaatt attatggatt aagtactagt ttagctgccg
     1981 ctgttgggcc atttttagga atgattttgc tgaatatgac tgattttcgt ttcattatct
     2041 ggttttcaat tgtactggtc ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa
     2101 atattccatt atctgaagaa caaaaaatat ctcttcgctc atggaatatc aatagtttta
     2161 ttgaaaagaa agcacttttc attacaggaa ttgccttttt gatgggtctt tcttattcaa
     2221 gtgttttatc atttttatct tcttatacaa aagtaatcca cttagttgct gctgggtcat
     2281 ttttcttcgt cgtatatgct ttggtcatca catttacacg tccattgact ggaaggattt
     2341 tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta tttattctta acagcaggat
     2401 tatttttact gagtgtaacg acgaatagtg tgacattgct tatctctgga gcattagtcg
     2461 gcctaggtta tggtacgttt atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg
     2521 aacatcgaat cagtattgct ttatctacct actttattgg tttagaccta ggcttaggcg
     2581 ttggtcctta tttgatgggg atgctaaaat ctcttacttc atttagagga ttgtatgtga
     2641 ttgccggagt cattccattg atatgtacag cactctatct tgttaatggc cgaaaacata
     2701 gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa
     2761 tagataaaaa aaaccgttca gatctgaaaa taatctgagc ggttttttga gtaaaaatag
     2821 tttatgatta ttcttaatta aacatctatt tatttttaaa gctttcattt atcgataaga
     2881 atagtttaac tgttgattta ttccattttc cgttcatctg gtacgatgcc ttgacttagc
     2941 aaccacttgt ggaagaacca ttctgccaca ccgatcaata gagcaggaat caatactttc
     3001 atccaaatat cgactgtatc attcataaaa tatagataga gccacaagat aactgcagct
     3061 gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt tacgtaagat caacagatcc
     3121 cctagcacat agatcatgac tacaataatt accccaacaa tcgttgaatc cataaagcta
     3181 acaccgtata aacctgtcag cacgacccac agaatcgccc agatcatgat tgccttcact
     3241 accaacgcct tcacatgttt cattttctgc actcctttct cctggataaa aaagtaagat
     3301 tatctaacaa gataaccttt tcattattat accatcggct gagaaaagcg agcaaagata
     3361 aggtcctatc taaaaatgag gaagccactt agaacattca cgtaataatt tatccgtaca
     3421 gacattcgat atggtgttcc tttactttcg catactgttc ttttgtcagt tctttacttg
     3481 tcaccagata atcgatttca tgaaaagcaa tggcttgatg catgaattta tgtgcaattt
     3541 tttgctgtgt aactaaaacg gcaagccggt ctgattgttt tctaaaagct ttttttgacc
     3601 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
     3661 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
     3721 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
     3781 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
     3841 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
     3901 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
     3961 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
     4021 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
     4081 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
     4141 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
     4201 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
     4261 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
     4321 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
     4381 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
     4441 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
     4501 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
     4561 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
     4621 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
     4681 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
     4741 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
     4801 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
     4861 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
     4921 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
     4981 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
     5041 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
     5101 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
     5161 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
     5221 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
     5281 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
     5341 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
     5401 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
     5461 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
     5521 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
     5581 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
     5641 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
     5701 cgaagtcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
     5761 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
     5821 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
     5881 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
     5941 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
     6001 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
     6061 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
     6121 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
     6181 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
     6241 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
     6301 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
     6361 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
     6421 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
     6481 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
     6541 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
     6601 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
     6661 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
     6721 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
     6781 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
     6841 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
     6901 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
     6961 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
     7021 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
     7081 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
     7141 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
     7201 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
     7261 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
     7321 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
     7381 tcgaaataac ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa
     7441 actatatact agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa
     7501 acgaagtttt aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc
     7561 taaacgttga gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat
     7621 tttttgttga aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag
     7681 aatggatgtg agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc
     7741 cattcaactg tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt
     7801 gaatccatga atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg
     7861 ctcctttgcc ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca
     7921 tcttaacatg tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt
     7981 taccaaagga gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca
     8041 aaaagcggat cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt
     8101 tccaaatttc ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt
     8161 ttttgatatc gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt
     8221 ctagatttcc agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat
     8281 cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc
     8341 cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc
     8401 cgcttggtgg aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga
     8461 taacgacatc tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa
     8521 ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc
     8581 ttcgttctag atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta
     8641 cttccggata gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg
     8701 cttttggcgg tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga
     8761 tcattcgggt agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa
     8821 gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa
     8881 cgctttccac gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc
     8941 ctgccacttt gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga
     9001 tatcttctgc acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa
     9061 cgatatccaa tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca
     9121 cagctagaga taattccttt ttattcatct tactgtaata aggaatttta aattctttgg
     9181 cataactata aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc
     9241 tcattcagct cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc
     9301 gataatatga tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc
     9361 tgccaatagt ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc
     9421 tgtgccgtga agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc
     9481 agcgcccatt cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat
     9541 gatttctgaa gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt
     9601 ggcaaatcct ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta
     9661 aacttcgatt tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat
     9721 aaagctttcc aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc
     9781 tgctaatgct agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc
     9841 atgtaatgtt tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc
     9901 ccccatatta ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc
     9961 aattactgtc tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac
    10021 tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata
    10081 catggcacca tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag
    10141 gtcaatcggt ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa
    10201 tttaccaagc agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg
    10261 cggcatcgga atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg
    10321 gacagttgcg cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg
    10381 cacaccttcc aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc
    10441 actatttttt gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac
    10501 gatttttttc atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt
    10561 ttatcataga taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga
    10621 gagcgacttc aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac
    10681 gctttgttag ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct
    10741 ttacctggag ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct
    10801 tcgatatatt tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact
    10861 tttgagatac ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca
    10921 tgtaatacta aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa
    10981 gctaatcctt tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag
    11041 tagtcgatac ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta
    11101 ccgatgattc cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct
    11161 tttgcatgtg ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga
    11221 tgtgaaccat cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg
    11281 taatcaccat ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa
    11341 tcagtgatta aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat
    11401 gtttggataa gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat
    11461 tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa
    11521 aattcagctc ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat
    11581 ttgttaagac aattcttaac caacgaccct attttaccaa acatttcggt agttttctac
    11641 taaaatcaat gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata
    11701 gcgctttaat cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat
    11761 cagaaaaaga gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt
    11821 tctatcaagt tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct
    11881 ttgattaatt tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc
    11941 acaaagaatt ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca
    12001 cctgaaaaga ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag
    12061 cggtgacggt gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt
    12121 ccttttttga ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg
    12181 ttttcctgat cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct
    12241 gatgacgcat cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata
    12301 ccaagacaaa tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt
    12361 ttcccttcta ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc
    12421 aaacgttctt ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg
    12481 tctgaattat aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct
    12541 ggtaactcga catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca
    12601 cgttcttcta attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg
    12661 tcgcaaacaa tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta
    12721 tctacatctg gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg
    12781 tttttcacgc tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata
    12841 cctaaaccac gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca
    12901 gctttcaagt aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct
    12961 gctttcattt gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc
    13021 cctacttctg taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct
    13081 ttgatctcat ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta
    13141 cgttctttac gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc
    13201 aaattgatgt caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca
    13261 tctgttacga atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg
    13321 tatggatcaa atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa
    13381 gaagctgcaa caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat
    13441 tttgtcataa gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa
    13501 acaaagctcc ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc
    13561 tctaaaaggg tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa
    13621 tgaatactat ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa
    13681 gtcctttatg agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta
    13741 acgttaaacc taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat
    13801 aaaaaaataa aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc
    13861 ctacttgcag caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta
    13921 tttacctcct ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc
    13981 tattaaataa tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt
    14041 caacaaaagt gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag
    14101 gagatgcgac aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc
    14161 ctcggtcaca agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg
    14221 tgattcggag ttaaatggta ggctcacaac ctctggataa acggtattca aaagccagtt
    14281 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
    14341 taatactcga aactaaacgt ctttttcaaa acctcttggt aaacggtgtt caaaagcttg
    14401 catctgcggt aataagttca gccaattcaa aaacaagaaa agctgttttt gcttggctat
    14461 tcttattact tgttgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca
    14521 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgttagt
    14581 ttctctaata ctcaggtcaa aacgctgatt tcacagcctc taattcacgg tattcctgtt
    14641 ttaataaagt tctagatatt gttctctttc ccactcggaa acagtttgtc tgaatgccgc
    14701 ccattcgaga cgttttgctt caacaaaatt tgagaagata tgttgtccta atgcgtcaat
    14761 cattacatta tccttacgca attctttgat agcattatgg atagttgaag gtaagtcctg
    14821 gatatgtgca gcttctcttt cttcctcatc cattacataa atgttacggt caacagctgc
    14881 tggcggtgtg atctgattac ggataccgtc tagtcctgct tgcaacaata ctgccattgc
    14941 caagtatggg tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg ataaaccgcg
    15001 agattctggt acacgtacta atggcgaacg attacggcca ctccatgcga cataaacagg
    15061 cgcttcataa cctggaacga gacgtttata agaattgact gttggattgc aaacggctgt
    15121 gtaggcacga gcatgtttca gcaaaccacc taagaaatga taagctgttt cacttagttc
    15181 caattcaccg tctttatcat agaaaacatt tccttcatca ttgaacaaag acatattgca
    15241 atgcatacca gaaccattga tcccaaataa cggtttcggc atgaaagtcg cgtgaagtcc
    15301 atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt tggatattat cacaagcatc
    15361 taccacatct gcatatttaa agtcaatttc atgctgtcct ggcgctactt catgatgtga
    15421 agcttccact tcaaagccta gactttctaa ttccaacacg atgtcacggc ggcaattttc
    15481 acctaaatct gttggtgcaa aatcaaaata accaccttta tcattcaagg tcgttgtgat
    15541 cccaccgtct tcgtctaatt taaataagaa gaattctggt tcaggtccta aattgaaaga
    15601 ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc gaggatcgcc
    15661 ggcaaatggt gtcccatccg gattatagat atcacaaatt aaacgagcaa cttttccatg
    15721 agcactttcc cacgggaaga tcatccaagt ggagagatct ggatacaaat acatatcgct
    15781 ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca aacatcattt tattttctaa
    15841 tactttgtcg atctggctca ctggaacttc tacatttttg atcgtaccta agatgtctgt
    15901 gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga tttcttcacc
    15961 tgttatctgt ttctttacca tggtatccct cttccttttt taatttattc agtcaaagaa
    16021 ttgtctttgc aatccttgcg actgataatg acctgttttg cttaacccac cttgcgccaa
    16081 aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct tgttttttgc
    16141 ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg atcccagcca tattcaatcc
    16201 ctctgaaaga tagtctttaa tctcaagcaa catatcaata tcattcagcg aatacatacg
    16261 tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt tcttcataat agcgaatttg
    16321 ccgagcagac aaatcagtca gcttcataac tgtaccaatt ggaaatacgg acatagatcg
    16381 gcgcaattcc ttttctccca tcagacgtcc cctttcgttt tataagttgg ctaaagtgta
    16441 ccaattaaaa aaaatattgt caactgcata tgttaggttt tctaacatga gatctctatt
    16501 ttttataaaa taacaaaaaa ataagaacac aaaaaaggta tctagttgat tttcgtacca
    16561 gatacctttt attttttatt atactgtact atcctttatt agtgttttta tt
//
LOCUS       DASHWH010000059        16534 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.062, whole genome shotgun sequence.
ACCESSION   DASHWH010000059 DASHWH010000000
VERSION     DASHWH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.062"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..565
                     /gene="scm"
                     /locus_tag="U2R41_002050"
     CDS             <1..565
                     /gene="scm"
                     /locus_tag="U2R41_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEM8889443.1"
                     /translation="STDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTST
                     TTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSST
                     SSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSN
                     NWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(720..1706)
                     /locus_tag="U2R41_002051"
     CDS             complement(720..1706)
                     /locus_tag="U2R41_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM8889444.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1729..2295)
                     /locus_tag="U2R41_002052"
     CDS             complement(1729..2295)
                     /locus_tag="U2R41_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEM8889445.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2309..2725)
                     /locus_tag="U2R41_002053"
     CDS             complement(2309..2725)
                     /locus_tag="U2R41_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM8889446.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3182..5116)
                     /locus_tag="U2R41_002054"
     CDS             complement(3182..5116)
                     /locus_tag="U2R41_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8889447.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5397..6353
                     /locus_tag="U2R41_002055"
     CDS             5397..6353
                     /locus_tag="U2R41_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEM8889448.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6443..7546
                     /locus_tag="U2R41_002056"
     CDS             6443..7546
                     /locus_tag="U2R41_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM8889449.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7559..8512
                     /gene="menA"
                     /locus_tag="U2R41_002057"
     CDS             7559..8512
                     /gene="menA"
                     /locus_tag="U2R41_002057"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEM8889450.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9326..10672)
                     /gene="gor"
                     /locus_tag="U2R41_002058"
     CDS             complement(9326..10672)
                     /gene="gor"
                     /locus_tag="U2R41_002058"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEM8889451.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10806..12059)
                     /locus_tag="U2R41_002059"
     CDS             complement(10806..12059)
                     /locus_tag="U2R41_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM8889452.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEEPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12065..12988)
                     /locus_tag="U2R41_002060"
     CDS             complement(12065..12988)
                     /locus_tag="U2R41_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM8889453.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13209..15701)
                     /locus_tag="U2R41_002061"
     CDS             complement(13209..15701)
                     /locus_tag="U2R41_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM8889454.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLAPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15708..16175)
                     /locus_tag="U2R41_002062"
     CDS             complement(15708..16175)
                     /locus_tag="U2R41_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEM8889455.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16311..16384)
                     /locus_tag="U2R41_002063"
     tRNA            complement(16311..16384)
                     /locus_tag="U2R41_002063"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16348..16350),aa:Arg,seq:acg)
     gene            complement(16442..16515)
                     /locus_tag="U2R41_002064"
     tRNA            complement(16442..16515)
                     /locus_tag="U2R41_002064"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16479..16481),aa:Pro,seq:tgg)
BASE COUNT         4882 a         3470 c         2942 g         5240 t
ORIGIN      
        1 cagtacagat actacctcta ccacaagtga tagcacggac acatcagctt caagtgacag
       61 tacagatact acctctacca caagtgatag cacggacacg tcagcttcaa gcgacagtac
      121 ggatacatct accacaacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga
      181 tactacctct accacaagtg atagctcaga ttcatctacc actacaagtg atagcacgga
      241 cacgtcagct tcaagtgaca gtacagatac atcctctacc acaagtgata gctcagattc
      301 atcaacttca agcgatagta cagacacgtc gtctaccaca agcgatacac gtcatgcaag
      361 tgtctccttc tcaaaaacaa gttcggataa caggacgaac tttaacttaa ctaataaaac
      421 aagcaatgac aatggaaaca aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa
      481 taattggata acactcgcag gcgttatatt acttgtaatc ggtttaagaa tcacttttat
      541 gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta
      601 acggacgttg ctctttattt ttgcttttta tcataaatac aagaactaca attcaatttg
      661 tcaaaaaaca cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt
      721 cagtctttgc gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga
      781 gaggtggcag gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac
      841 ttcctagctt cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct
      901 tcttctgtca ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc
      961 aaagagtaga tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg
     1021 ccgatttcct ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc
     1081 ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca
     1141 aaagcgccaa cagaacaact taattggaac agttcagctg tcttacctga aatattttcc
     1201 aaatattctt ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca
     1261 ttcaatactt tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa
     1321 tcagacaata gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca
     1381 ttaccaaact gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata
     1441 tcatcatgga tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct
     1501 ttgtctcgat cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac
     1561 agctttccgc cggcatggat cgtttctttg atggcttgtt cgacaggttt gtttttcagg
     1621 cggacagttt cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag
     1681 ttttcccaca ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt
     1741 aaacattggg ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag
     1801 tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat
     1861 ccctaaaact gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga
     1921 agccatcaaa agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat
     1981 actgacacgt tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc
     2041 accactcata ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag
     2101 tcgtagccaa agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt
     2161 gattagattg gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat
     2221 attttccaat aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt
     2281 ttgtaattta ctcatggtgt gtgaaggtct aataaatcag actgtcttcg tcacctccat
     2341 ctgttgaacg gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt
     2401 tcgttgccca gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta
     2461 ctcgatcgcc atctatctcg atcaagttat aatctccgtg ttcatctttg taagtatatg
     2521 tgttgctctc ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca
     2581 gtaccgcttc tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta
     2641 aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt
     2701 tttcagcaaa ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat
     2761 tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag
     2821 aatagaaaac agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg
     2881 tggaacaccg tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag
     2941 gaatttacga aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc
     3001 tacttctgga acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt
     3061 gctcatggag gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt
     3121 gacggtttgt ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta
     3181 tttatttctt gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt
     3241 gtagccatgg aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg
     3301 cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg
     3361 tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg
     3421 ctaatgccaa ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat
     3481 tgtttggcat gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag
     3541 ccattggttc ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt
     3601 ctccggcact tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac
     3661 cactggcaac atctgttaca ttgtattccc atgtccaagg gaacacactg tttccagtga
     3721 accatgatcc ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt
     3781 ttttggaagc ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata
     3841 agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga
     3901 aaaactcatg cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt
     3961 tcaaattcac gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa
     4021 ataggttagc taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt
     4081 taaatttatg cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc
     4141 ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa
     4201 agactaaatc accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt
     4261 cattggcgat caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag
     4321 ttcctttgac accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca
     4381 atttgatatg atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt
     4441 gcttacgcaa atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg
     4501 tcggcattgc ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg
     4561 ctaggcgatt tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac
     4621 cacagacaac gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt
     4681 ttgctactgt cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg
     4741 tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa
     4801 ggatcagctg atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat
     4861 cgataccgat tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct
     4921 gcagatcgta ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg
     4981 tcatcatagt atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca
     5041 gtttctttgc caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa
     5101 tattttgctt tgccattgat catccgctcc ttatagacta tctatataat gaatatatga
     5161 taattctttt agtttgatac ttattacaca atctccatat attatataga tgttctgttc
     5221 ttttgtctaa ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa
     5281 tttgctgaaa gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta
     5341 taggctaaaa agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg
     5401 aatggtgggg tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat
     5461 tttcagctct agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt
     5521 ctgaatcatc tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag
     5581 ttgtagctgg cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa
     5641 atggttaccg tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat
     5701 atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca
     5761 tggaagcaaa atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct
     5821 taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa
     5881 gcttccaaaa ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa
     5941 tcgaaatcga caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact
     6001 actctaacgg ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat
     6061 caaactttga ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg
     6121 aaaatatgaa agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg
     6181 aattcgtcaa agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag
     6241 gtgctacaca cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg
     6301 aaaaaggcga cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata
     6361 atcatactaa taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt
     6421 tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg
     6481 catctctttt atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag
     6541 ttcagcagct accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac
     6601 ttacgttcgt atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga
     6661 tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga
     6721 agtgaatgag caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt
     6781 caagcgggcc tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc
     6841 aatcacacaa ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat
     6901 cgatcaggca ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac
     6961 tgtttatcta aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat
     7021 tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt
     7081 gggcggtaat gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt
     7141 cggcatccaa gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa
     7201 caaaacatta gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata
     7261 ccatcatttg tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac
     7321 gatcataaca gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg
     7381 tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt
     7441 cgtgacaaaa gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc
     7501 ggaggactcc cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat
     7561 gacactaaaa atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc
     7621 ttttgcggta ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac
     7681 ttttttattt tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta
     7741 catggattat gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg
     7801 cagaaatcta tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt
     7861 tgttatcggc gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat
     7921 ctgttgtttt attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt
     7981 aggggaaatt ttcagtgggg tgactatggg ccttggaatt tttgccatgg tcgtttatgt
     8041 caatacattc ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttga
     8101 tttggtagga aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt
     8161 tacgattgcc aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa
     8221 tcatcgctat acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct
     8281 attgatgtat gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg
     8341 gccgatcttg gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa
     8401 gcaaacgtta ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa
     8461 tgccagttat gtattggggc tattactaac aatcattatg caagccattt aacagcatga
     8521 agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc
     8581 cttatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     8641 tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg
     8701 ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt
     8761 tatcagaggt tgtaaaatca gtgttttgtc ctgagtatta gagaaacaaa tggaaaaata
     8821 gttcctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacactatt
     8881 tgttcggaaa aagtgactaa ctatgacaca atttttttca cagtcccttt tttcacttga
     8941 tttgattcgt accagcgaac ttgacgagaa ctttcttggt ttggtaacta tatggtattc
     9001 ttttaacatc ctcgtttgct tcttctaatt tatttataca aagtcacact tttttaaaca
     9061 tctgttcttc gatacatttc tatttctttt aaacatcgat accaatattt tgaacttaag
     9121 agtgcatacc aaatggattc atttactctt cgtcagttac gaagataatc aaaatctcca
     9181 acttccctac ttatttcgtt ttgagaatta tcgaagcaat cttacataaa taactaaaaa
     9241 acaaaaaacc agaaaatcag tatactgatt ttctggtttt acatggtttg ttcaataaaa
     9301 agctgttgga actgttaata ttaaattatc gcatcgttac gaattcttcc gctcctgttg
     9361 gatggatggc tactgtattg tcaaagtctt ctttagttgc acccatcttg atagccacgg
     9421 caaaaccttg cagcatctca tcgacaccaa caccaatagc atgtaagccg acgattcttt
     9481 cttcttttcc gacacagatc aatttcattt cgcatttttg gcgataatca tttaatgcaa
     9541 agtacattgg ggtaaagcgt gaacgataaa ctttcaattc attttcgcca tatttttcca
     9601 atgcttcttc ttctgtcaaa ccaattgttg caattggtgg atgcgtaaag acgactgtcg
     9661 gaacaagttt atagtcaaga taagaatctt tctttccatt aaataaacgt tctgataaac
     9721 gtctaccagc tgcgattgca acaggagtca gatctaattt accgatcaca tcaccaacag
     9781 caaaaatatg ttcatccgtt gtattttggt atttatctac tttgataaac ccttttttgt
     9841 ccaattccac atttgtattt tccagaccta atttttcggt attcggtaca cgtccaccgg
     9901 caaaaagtac acaatcacca gttaaggttt caccattttc aaatagcacc gtatattcgt
     9961 cattgttttt agtaatttct ttagcaatat gatgtgcgta agtttttatt ccagcttctt
    10021 ggtacatttg gatcaaattg tccgaaagca ttttatcaaa cgttctaagt ggtcgttctt
    10081 ttcgatatgc ccacatgact tcactaccta aaccacgaaa tacacctgaa agttcagccg
    10141 caatgtatcc agcacctaaa acgattgctc tcttaggtaa ctctgttaat tcaaaaaatc
    10201 catttgaatc aattgcgtat tctccaccag gaatggtcat tttacttgca cggcctccag
    10261 tagcgatcaa gattttagga gcacgatagg tttcaccgtt tacttcgatc gtttgactat
    10321 cgataaaagt agcgtaacct tttatagcct cgacatgatt gctatctaat cctctttgat
    10381 aagcaccgtg tagaaaatca atatattttt ctcgtctttc tactagaccg gcgaaatcaa
    10441 cattttttaa atctgcttgg ataccgtaac ttgaggcatc tcgttcaatt gtttccaaaa
    10501 tcgtacttgc ttgccacata acttttttag gtacacaacc gacgttgaca catgttccgc
    10561 ccaattcatt cgcttcaatc agcaaaactt ttgcgccatg catccctgct ctgtttgctg
    10621 aagcaatacc gccagagccg ccaccaatta cgatgtaatc atatatttgc attttgttcc
    10681 tccttattga agagatcaat caaaaccttt atttaacgat cctttttcta cagaaaacaa
    10741 gtgtaacact tttcttgaat tttgtatgcg aagatgctta aaacagggat atatcagctt
    10801 tttccttatt tacgttttcg gatcatatct ccaggtttca ccggctgtgc gactggcata
    10861 gttaaaatca tcattggatt tggtgctcgg tcaatggatt cgccttcttc attatgcata
    10921 acttcaacgg tttgactgaa atgactgaat cctggaccat aaaattcgat ttcatctcct
    10981 acagaaaaca agtttctctg acgtatcgta gcaatctgtg ttgcctcatc ataagataat
    11041 acttcaccga caaatttata tactgggatt ttacgacgtt caccgaataa ttgttcgttt
    11101 tcagttggtg tattgtagta aaaacctgta gctaattctc gttgtgctac tttccataac
    11161 tcgtcgatcc attcttgctt gcagacatag ttttcaggtt cttccatata actgtctaca
    11221 gccgctttat agacatttga caccgtagat acataatgga tcgatttcat tcttccttcg
    11281 atcttgaagc tgtctacgcc gttttcaatc aattctggaa tatgttcgat cattgacata
    11341 tccactgcac tcatcgagaa ttcttcttcc acttcgcctg tatcagttaa actgtttcgt
    11401 tcttgaccaa aaggcatatc gaacaatcca tacttccagc gacaagattg agaacatccg
    11461 ccacggtttg catcccgcat cgacatatgg ttggacaatg tgcatcgacc agaataagag
    11521 atacacatgg caccatggat aaatgcttcg atttcggtat ccgtattttt acgaatctct
    11581 gccacttcct ccattgaaac ttcacgagct aaaacgacac gttccaaccc ttcttctttc
    11641 cagaattcca atgtctcaaa attagttgct gaagcttgag tggataaatg gatcggcaga
    11701 cctggggctt ctgtcgcaca aatttcgatc aaagctggat cagaaacaat cactgcagaa
    11761 ataccgacat cacgcaaact gcggaaaaat tcccctgctc catcttcatt tccttcatgg
    11821 gtgaccatat tagcagcaac gtataccttc gcatcatgtt cttttgcaaa agcaactcct
    11881 tcagccattt cttcataagt aaaattacct gcacggcttc ttaaaccata tgcattcccg
    11941 ccaatatata ctgcatctgc tccataatgg atcgcggttt ttaacttttc cagtgttcct
    12001 gctggcgcta ggatttctgg tcgtttcaat tttcttttta ctgtcatttc tttcatcata
    12061 ctccttattt gatagcagaa ggatcaatat aaaagaatcc tgtatctaat tcacggtttt
    12121 caggatgcaa tttactgatt tcgctagtca ttttttccgc tttttcttga tcccagctac
    12181 cttctaaaat ttgttttttt gcttcggcga atagtcgagt gatgtttacg aatttttcgc
    12241 ctggagtaaa taatccgtcc aatttccatg tacagtaatg atgctgataa agttcattca
    12301 attcattcga gagattcaga tcattgctgg caaaaatatg tgtgccatga ctatcttcat
    12361 aaatagaata atgtgtctct tcttttttag gttccgagat aaaaagacct ctttcacgat
    12421 ctttttgttc gtcttgtttc gtatagttat agtaattctg tagcagtgga cgtttggatt
    12481 gatggatgca tgtagcacca tacacaaggg tttctacagg aatatcgagt ttttcttcca
    12541 tcgccttcat ctcttcaaaa ggaacttcac gagcaagtac tgctccaact gcgccttttt
    12601 tcgcccagaa attgatttga cgtgcgctcg ttactaatgt ttcagcatca taaatatacg
    12661 gtatacgcaa ttcaggattt tttttcatcg tataaataat acctgtatca ccaactgtga
    12721 tcacatctac gtttatttct tttaaaaaag ccaaatattc aggtacattt ttcatttttt
    12781 caggatgaat gatcccattt acagcaatat tcgctttttt accatacttg tgtgctaatt
    12841 ctaccaattg tcgctgttct tctcgtgtaa atgaagcagg cagacgcaat ccaaattgct
    12901 cttcaccgaa atatagtgta tctgcacctg cagcaagcaa ctgttctgct tgttcaatag
    12961 tttctactgt ggttatgagt tcgatcataa ttttctcctc ctacaacttc ataatagtag
    13021 catagctcta aaaactttcc aatacttctt ctatttaaag tcggattttt gaaaattatt
    13081 tcacaaaaaa tcaaaataaa gttcgatata ttctgtccaa atataaaaag agattgtgac
    13141 aaaaatcttg tcacaacctc ttaatcaagt cttactatta taaaaattca gctacacagg
    13201 ccaagtaact atagggaacc tactgtttcc tttttaggtc cgcgaacatt caatgtgatc
    13261 ttaccttttg aagcacctac tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa
    13321 gcctcgctca atcgatcttc aatttctttt tgaagtgcgc gacggattgg acgtgcacca
    13381 tattcaggat caaatccagc tttcccgatc acatcgattg cagatggtgt gattttcact
    13441 tgaatatctt gttctttcaa tcgtttgata atcgctttgc tcatgatttg gacgattgta
    13501 tggatttcat ctttactcaa tgaatggaag acgacagtct catcgattcg attcaagaat
    13561 tcaggtcgga aagctttctt taactcttct aagatacgtt tttgcatggc tttgtgatct
    13621 ttagtaacat cttggacatt gaatccgaca tttttttctt ctcgaatttg tgtcgctccg
    13681 atatttgaag tcataatcat gattgtattt ctaaagtcca cttttctacc tttagaatct
    13741 gttaaatgac catcatccaa tacttgcaga agtaaattga aaacgtctgg atgtgctttc
    13801 tccacttcat ctaagagaat cactgaatat ggtttttgac gaattttttc tgtcaactgt
    13861 ccaccttcat cataccctac gtatcctgga ggagacccga ttaatcggct tgtactgtat
    13921 ttttccataa attctgacat atcaacacgg atcagggcat cttcacttcc aaacatgact
    13981 tctgaaagtg ctttggccaa ttctgttttt ccgacaccag ttggtccaag gaacatgaat
    14041 gaaccaattg gacgatcagg atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa
    14101 actgctttga cagcctcatc ttgaccaacg acacgttcat gcagaagtcc ttccagttct
    14161 aacaagcgtt cgctttcttt tttctccaac tgctgtagag ggacaccagt ccactccgat
    14221 acaactgtag caacatcttc ttctgttaca cgatcagaat aaccttgctc ctgttttgct
    14281 tcaattgcag aaactttttg cagcttttta cgtagtgctt tttcttgtct gcgtaattga
    14341 gcagcatttt caaagtcttg tttttggatc gctgcttcct tttcttgtac tagttcatcg
    14401 atttctagtt tgatcacagt tgattttgtt aagtgatcgg tttgatccaa acggactttc
    14461 gctgctgatt catcgatcaa atcaattgct ttatcgggta gttgacgaga agtgatataa
    14521 cgaacagaaa gattgactgc tgctcgtaca gcttcatctg tgatctccac tccatggtgc
    14581 tcttcatatc ttgtacgtaa tccttgaaga atcacttctg cttcttcagg agttggctca
    14641 tctacttgaa tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat atatttttga
    14701 tattcatcta gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc
    14761 aagatattcg aagcatcaat tgctccttct gctccacctg caccaattaa ggtatgcaat
    14821 tcatcaatga ataagattac ttgaccatca ttatagattt cgtcgatgac ctttttcatt
    14881 cgatcttcaa attcaccgcg atatttcgta ccagcaacca aagatcccat atcaagcatc
    14941 atcaggcgct tttcttgcat gtcttctggc acttcaccta aaatgatttt ttgagctaac
    15001 ccttcagcaa tggctgtttt gcctacacca ggttcaccca ccagtacagg gttattcttt
    15061 gtccgtcggc taagaatctg gataagccgt ttcacttctc tcgaacgacc aacaactggg
    15121 gccagcttgt tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt
    15181 gttccttgcg gtgtttgccg tttatcttgt acatttacat ttctacgacg attcattcct
    15241 tttccagcag aatcaccagc tagccccatt ttttgtttca gaacttgacg ggcttttgaa
    15301 aggctgatac ctaagttgat catgattcga gtagccatga tactttcatc ttttaataag
    15361 ctcatcaaaa tatgttctgt tccaactttt tgagaaccag ttttcttcac ttcgctgcta
    15421 gctaaagcaa aaacttgttt cattcttggt gaatatggaa gaaagactcc tttagggtaa
    15481 gaacggatac caccataacc aatcagatgt tcaatttctt cataaatttc cgtttcattc
    15541 aaatccattt cacgtaatac ttttccagca attccatttt gctcgaccac taaggctagt
    15601 aaaacatgtt ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt
    15661 tctaatactg cttgggctct ttcagtaaaa agttcttcca tctaatctca cttcccatct
    15721 tcataactta aacgttccag aaatgctcgg agaatccgag cacgtgtatg atcttcatat
    15781 tcattgaaat tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt
    15841 gtaattattt ggtcttcata aagtttttga ataatagcaa aagcattttt ctcactgatt
    15901 gtatcatcaa ataactgatt tatctgctca agtaattgtt ttttatctga aatccttact
    15961 tttgcaattc gtatataacc accaccacca cgtttgcttt ctactagata accacgttgg
    16021 atggtaaaac gggtattaat cacatagttg atttgtgaag gaacacagtt aaagaggtct
    16081 gccatttcaa tccttcgaat ctcgatcatt tcactatttt ccagaatttt ctttatatat
    16141 gattcaatta aatccgaggt attctgatgt gccatcttat tcattccctt tgactaatat
    16201 tgaccttaat tataacgaat ttgataatag aaagaaagaa gaacgctttt tgttaaataa
    16261 atcctttaga atcaacaaaa aaaaatacgg aatatactat tccgtatcga cgcgcccaag
    16321 aggagtcgaa cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg
    16381 gcgcatattc aatttaaaaa aattataaat catttgaaaa caaaaagcaa gataaaaaaa
    16441 atcgggaaga caggattcga acctgcgacc ccttggtccc aaaccaagtg ctctaccaag
    16501 ctgagctact tcccgtttaa aaaataaaaa agac
//
LOCUS       DASHWH010000060        16527 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.063, whole genome shotgun sequence.
ACCESSION   DASHWH010000060 DASHWH010000000
VERSION     DASHWH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.063"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(141..416)
                     /locus_tag="U2R41_002065"
     CDS             complement(141..416)
                     /locus_tag="U2R41_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEM8889456.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="U2R41_002066"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="U2R41_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEM8889457.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="U2R41_002067"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="U2R41_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEM8889458.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="U2R41_002068"
     CDS             complement(3515..4195)
                     /locus_tag="U2R41_002068"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEM8889459.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="U2R41_002069"
     CDS             complement(4244..4879)
                     /locus_tag="U2R41_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8889460.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="U2R41_002070"
     CDS             complement(4945..6348)
                     /locus_tag="U2R41_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEM8889461.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="U2R41_002071"
     CDS             complement(6335..7366)
                     /locus_tag="U2R41_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889462.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="U2R41_002072"
     CDS             7397..7618
                     /locus_tag="U2R41_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEM8889463.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="U2R41_002073"
     CDS             complement(7783..8520)
                     /locus_tag="U2R41_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEM8889464.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="U2R41_002074"
     CDS             complement(8673..10013)
                     /locus_tag="U2R41_002074"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEM8889465.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="U2R41_002075"
     CDS             complement(10250..11773)
                     /locus_tag="U2R41_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEM8889466.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="U2R41_002076"
     CDS             complement(12151..12762)
                     /locus_tag="U2R41_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8889467.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="U2R41_002077"
     CDS             complement(13092..13808)
                     /locus_tag="U2R41_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM8889468.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="U2R41_002078"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="U2R41_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEM8889469.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="U2R41_002079"
     CDS             complement(14367..15164)
                     /locus_tag="U2R41_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEM8889470.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="U2R41_002080"
     CDS             complement(15134..15505)
                     /locus_tag="U2R41_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM8889471.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="U2R41_002081"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="U2R41_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEM8889472.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DASHWH010000061        16481 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.064, whole genome shotgun sequence.
ACCESSION   DASHWH010000061 DASHWH010000000
VERSION     DASHWH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.064"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            546..770
                     /locus_tag="U2R41_002082"
     CDS             546..770
                     /locus_tag="U2R41_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889473.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            788..1216
                     /locus_tag="U2R41_002083"
     CDS             788..1216
                     /locus_tag="U2R41_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889474.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1194..2612
                     /gene="terL"
                     /locus_tag="U2R41_002084"
     CDS             1194..2612
                     /gene="terL"
                     /locus_tag="U2R41_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEM8889475.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            2624..4159
                     /locus_tag="U2R41_002085"
     CDS             2624..4159
                     /locus_tag="U2R41_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8889476.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4239..5117
                     /locus_tag="U2R41_002086"
     CDS             4239..5117
                     /locus_tag="U2R41_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEM8889477.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5119..5436
                     /locus_tag="U2R41_002087"
     CDS             5119..5436
                     /locus_tag="U2R41_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889478.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            5563..6186
                     /locus_tag="U2R41_002088"
     CDS             5563..6186
                     /locus_tag="U2R41_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEM8889479.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6201..7091
                     /locus_tag="U2R41_002089"
     CDS             6201..7091
                     /locus_tag="U2R41_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEM8889480.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7114..7329
                     /locus_tag="U2R41_002090"
     CDS             7114..7329
                     /locus_tag="U2R41_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889481.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7341..7682
                     /locus_tag="U2R41_002091"
     CDS             7341..7682
                     /locus_tag="U2R41_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889482.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            7682..8053
                     /locus_tag="U2R41_002092"
     CDS             7682..8053
                     /locus_tag="U2R41_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889483.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8046..8441
                     /locus_tag="U2R41_002093"
     CDS             8046..8441
                     /locus_tag="U2R41_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM8889484.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSIGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8443..8820
                     /locus_tag="U2R41_002094"
     CDS             8443..8820
                     /locus_tag="U2R41_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889485.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            8821..9429
                     /locus_tag="U2R41_002095"
     CDS             8821..9429
                     /locus_tag="U2R41_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8889486.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9429..9770
                     /locus_tag="U2R41_002096"
     CDS             9429..9770
                     /locus_tag="U2R41_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889487.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10012..12801
                     /locus_tag="U2R41_002097"
     CDS             10012..12801
                     /locus_tag="U2R41_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEM8889488.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            12791..13531
                     /locus_tag="U2R41_002098"
     CDS             12791..13531
                     /locus_tag="U2R41_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8889489.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            13528..16239
                     /locus_tag="U2R41_002099"
     CDS             13528..16239
                     /locus_tag="U2R41_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEM8889490.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            16252..>16481
                     /locus_tag="U2R41_002100"
     CDS             16252..>16481
                     /locus_tag="U2R41_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM8889491.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         5875 a         2352 c         3437 g         4817 t
ORIGIN      
        1 tggcggtttt ttggcggaat gatggcggtt tttagccatt tatcagtgat attatggtag
       61 tgtcgaaaga ttagtgatag gtctgagaca aaataataat aaaaggaaca tcgttttatt
      121 attgtttcac aattaagctt cgatagacag caacggaaat attaagaata aggatgtgaa
      181 ttttaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttacata
      241 cagatcgctt aggcggtctt tttattttgg ccgaaaacct acattttcga tggccgatta
      301 ttggaggagg aatagcatgt tcaaactatc tgaaatcatt aagaagtccg acgttgagaa
      361 attagagatg ttaaaaaaga agttgaagaa atagcagtct cttcgtgagg ctgttttatt
      421 ttgctcacaa aaatagacca ctatcgggta atagtggtca ggaattaaat gaaaaagatg
      481 ttagggttgt tagctaagta taacatcata acgcttacaa aacaatgcaa gaaggataaa
      541 ttattatgag aacttactgg tatgtgtcat taaacaataa atatccgaag ccaatgaaag
      601 gacagcatag gcgtgtagtc atgtctgttc aaatgaaggc gaagtattcg attgtagaaa
      661 tgatcagaga ggcaacgcca gtagagattg attattgcaa gctagtctat tgtgggtgcg
      721 gccgttggaa agaggatcat gtacaaaaaa atatcagcaa gtacatatag agaggcggtg
      781 attaaggatg gaaaaaagcg atacgaaagc gaacagatac aaacctactg ctgcagagaa
      841 aaaactcttg gaagtattga taaatccaga taacttaggc aaaccggtgc aagagttgtg
      901 ttcactggcg aaaataggta ggaccaaata ttacgacgcc atgagcaaag atggctttgt
      961 tgatctagtt aacgaaacaa caatggatct gatcaaagga aaagcttctg atgtccttaa
     1021 tgccacttac aagtatgcat tgactgaaaa aggtcatcag gataggaaaa tgattctgac
     1081 tattgctgga atctatgcag acaaacaaga actcaaacat tctggcggcg tcgatatccg
     1141 aaaacagtat gagaaaatgt ccgatgaaga attagaagag ttggtgaaac ggtatgaaaa
     1201 aatcaacgac tcttaatcgt gttgagtatg ccgttgttca aagagagcta gccattcgaa
     1261 aagctaggaa aagtttttgg ctattctgcc agcttcttta tccagagttt tacaaagatg
     1321 atcggttgta tttgaaagat ttatgtcata cgctacaaga tttttacgaa gataaaatcg
     1381 acaaaaaaat tttggttatt aacatgccgc ctcgacatgg gaagactttt acagcgcggc
     1441 tattcgtttt atggatgttt ggacaaaatc caaagacgaa aattattacc ggatcttata
     1501 accaaatcct gtcatcttta tttgcgcaac aaacacgtga tggaatcatg gttgaaaacg
     1561 aaaatgttaa ggctgaatac tttaaagata tttttccaga ggtgcaaatc aaatatggag
     1621 atgctgcaaa gggtttctgg tctttagatg gttcagaaga aaagaattat ttagcgactt
     1681 ctcctggtgg aacatccact ggtattggcg cgaattatgt aattgttgat gacatcatta
     1741 aaaccgcaga agaagcctat aacgagcgtg ttttagattc tcactgggaa tggtataaca
     1801 acacgttggc ccaacgaatg gaacggccac gtaaacagat attgattatg acacgttggt
     1861 caagtaatga tttagccgga aaaatgttga cgagacgaaa aaataatgtt catcagatct
     1921 gttataaagc agtacaagaa gacggctcga tgttgtgcaa cgagattatg acccacgatg
     1981 agtatttaga tgtggtacaa gaaatgaatg tagatattgc agaagccaac tatcagcaag
     2041 aaccaattga ccaaaaaggc aggctatatc agaagtttct tacttatgat acgttgcctg
     2101 ataacattat caaaatatgg aactacaccg acaccgccga caagggtgcc gattactttg
     2161 cgtctcctgt ttttgctgag acgtctgatc atgatgctta tattatcgat gtcatgtata
     2221 caaaagagcc gatggaaaca actgaaatag cccacgctaa tatgattatt cgcaatcgag
     2281 ttaataaggt acggattgaa ggaaacaatg gcgggcgtgg attcagaaga aattcagaaa
     2341 gaattacaaa agagcgaggc tatcgaggtg cagtatttga agactttcat caatcggata
     2401 ataaactgtc tagaatactg gcgaatagtg cttgggtgga aaatcatgtt tactatccag
     2461 atgattgggc gacacgttgg ccggactttt atgaggctat ggttagttat caacgtgaag
     2521 gtaagaacgc tcatgatgat gctccggatg cagttacagg aattgcagaa acgattatag
     2581 aagcagatga cttcgagttt attgcttatt aggaggtggg tctttgtttt attctggaat
     2641 cacattgaaa aaatacaaac gtttaagaac gaaatattca acgcaaatcg aagaaggatt
     2701 attcgatcct aacgctttta tggaagatat gaaacccttt ttcgatgatc gagaaagaaa
     2761 gtatcttgct tatactagcg agaaaaacga gatcgacagt cgtgagaaac caaaaacaaa
     2821 aatcattaag gtcaataaca agattcatgc gggaatgtat tcagtggtcg ttgatcaagc
     2881 agttaaccat ttcacaggaa taccaattaa atgggattat gatgtttcgg aacagaaaag
     2941 gacattcatt caacgaatga gagacaaatt actgggtaat gatatcagcg ccccttctac
     3001 cccagaagaa tttgaaacac tcacgagaca attaagtact atgcggtttg gaatgttaga
     3061 ccccgatact gcaacatttc aaggagcgac agggcttgct ttcagattac tggagcctgt
     3121 tgagacaaat gatggttggg aactaagagc aagcaacatt gagccgtgga aagctgaaaa
     3181 gtacgaaaac ggcgcagtgt acattaaaga gaaatacgat ccatatcaaa agaagtttta
     3241 tgaagagatg aaggttgtca cgccgaaatc aatatttact tatgctcgat atactgatta
     3301 ccctggcacg gtacaaggag catttaaaac gctgggtaat gttcctaatc ctttaggcgt
     3361 tatcaatctt acggagttta aaaacaatac aaaccgttac tgtgactttg aagttgcaga
     3421 ggagattagt gatgctattg atcgtgtttt atctgatcag cagaacgaga ttgaacaatt
     3481 caagttagct tatatgctga tcactggggc agatttgtcc gaggataaag ccaagaagat
     3541 gatggagcaa ctagggatca tcaatctgaa agaccctaat gcgaaagccg gctacgtgac
     3601 aaaggatcta caaaaggatt tcaacgagta tcattttgat cttcttaaaa agcaattcta
     3661 cacggtgtgt aaagcaattg atttcaatga cgaagtcttt aagtcgaata gttctgggga
     3721 agcccgaaag tggcaaatca ttagtttaga agctaagaca aataccaaag aacagtactt
     3781 tcgagaagga ttgaaagagt gtgccgaggt tatggctgct tatttggatt ttgccgagaa
     3841 agttacaatt gatccggaaa agatcatttt caccttctct cgttcgcttc caacagattt
     3901 aagctaccta gctgaggcat tgcctaagtt ggcaccatac gtttctaaga ggacgattca
     3961 aagtcagatc ccgttcgtca cagatgttga ttatgaaaac gaaatgattg acgtcgaaag
     4021 cagacaatct tacccagacg gcgagtatgg aggtggcatc gttgagtcaa atagaaacga
     4081 agtactggac caaacgtcga gaactggaag atttagcaag actcaaaatg gaaaatcaaa
     4141 cgctaaagaa actaagtagt gtctttcctg atgcgttgaa agaaattcaa gcggagttat
     4201 tatcccaagc tgatttacac gatattacct atgctgagat gatggaattt tatagtacaa
     4261 gtgatcagaa aaaatatcgt gaatacgttg aaaagaacta caaatcactg aagatgtatg
     4321 atgcaaaata caaagagttc atcgaagaat actttccttc tttcgactac gccaaagtga
     4381 atcggctgct tcaaatgagg tcagatattt ttcgaattct tgcagagagt gcaatagatg
     4441 cagatgtgaa taaatatttt tcggatcggt tagaagatat cttgcaacgt tcgtatgtat
     4501 caaacgcaaa cgtatttgcc cagattttga acgtagattt gccgaattac ttatcaaagg
     4561 aagaacttga acgttacctg aactatcctt ggtcaggtaa gacattctcg cgtcgtttat
     4621 ggggcaatat ttcatctcta gaacaaaagc tatctaacgc cattacaact agtgtagcaa
     4681 gtggcgaagg agttctcgtg gcattacgaa caatgcgaga agacccagaa atatgcgata
     4741 tgttcaaaag ggaggaatcg aagtacaaca aggcgatcga aaatcttgtg agaacagaat
     4801 acgctaaatt cgctcaagat ggtattgaga aatcttatat tgaaactggc gtagacgaat
     4861 acaatgtctt aactgcaaaa gatgaaaagg tatgttcaat atgcggtggc aaagcaaaga
     4921 acaacccata caagttgagt gaagcgatca ttggtgagaa ccgtgcgcct tttcatggcc
     4981 gatgcagatg tacagatgtt cctaatatgc cgaaattagg aaaagatatt gacgaagagt
     5041 atgagcgttt atttggcgat ttattagatg agtttgcaca tgattctttt ggaattaatt
     5101 tgaaacggag gaagtagaat gaaagatttt tttgaagcag tactaacaat taatgtaaat
     5161 gctgatattg cagaagccta caaaacagct attgagtctg agaaccatcc taatggcttg
     5221 agagaccatt ggaatggcaa ttatgcctac gtggttattg gcgatcaaac tgttaattat
     5281 caagataata ctccagttga taagaatacc gttaatttaa cgattcaatt attatctcat
     5341 tcattaccaa atttaaaaga aacagttgat tggtatgaaa atatgggatg tattgttgtt
     5401 agaactgact acaaagaagg aaagtctagt aattaggctt ttttattttg tccgaaatga
     5461 cgttaaacta gcgcaatact gggcttggtt gaatggtggg gcgcaactat taaaactcaa
     5521 agcaatgcgg ggcgtgaaaa cgaatcgtgg ggcgaaagga gaatgattat gaaacacaaa
     5581 tcattaatgc caatgaattt gcaatacttt gccgaaggtg acgatcaaaa gttttctttt
     5641 gatgacttca aatcttttgt ggaatccaat gaagaagctc aaaaatttat tcagtcacaa
     5701 tctcaaagtg ttgccgacaa acaattggaa gcttggaaac aaaataactt agataaaatt
     5761 aaacaggata ccatcaagga atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt
     5821 caactggaaa aactacaagc tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat
     5881 aataaagcat ttgttgcaga acaaattgct ggattagaac tagatggaga gttaaaagag
     5941 tctatttctc aatttatgct aaataatctt gttagttcgg atacagattt cactaagaat
     6001 gctgttgaag gttttacaag tgttttgaat gcaattaatg agaaacatgc agatgcatta
     6061 aaagaactac aaatgaagtc tgcatttggt ggaactcaac aatcgaataa ccaggttcag
     6121 cagaacaatg aaacattaac aaatccagaa gaacaattag gacaaattct tcaacaattt
     6181 aactaggaga gtgaaaaatt atgaaaaaaa catctttaaa taatttagag tatttggata
     6241 tttcaccagc gattaatgct atgcaagtac caaatacacc ttttttaagc tatttacttg
     6301 gtgctggaaa aacagagcca gcaaactcga cagaaattaa atggcgtgaa tatgatatca
     6361 acaacgatga ttcttctgaa aaacttgagg gcggagaata tccagatgct gaatcaggtc
     6421 gaacttggtt taacaactat actgaaattt ttagaaaatc aacctctgta tctggtacat
     6481 tagatgctat taatgtgaat ggagtcggaa atgaattaac taatcaagta gcactacgtg
     6541 gtatggaaat gaaaattgat ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa
     6601 atggttctaa aggtcgtcga atgaatggga ttttgaactt gatcaattca gcaaataagg
     6661 cagaaacagc cactgcgggt gcagtaacaa gaaaagatat cgatacttta tttaaattga
     6721 tgtatgaaaa aggttatatg ggagaaaaac tatgcttgat ttctccagat atgcaggagt
     6781 taatgactga tgagttagat ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa
     6841 cttttggatt gcaaattgga aatatcgtgt ctaattacgg tacaggtatt gctctactag
     6901 aaccatcatt gccaaaagga acaattgccg caatcgatac taattatgtg aaactacgtc
     6961 cattacgtga atggagagca gaagaacttg caaaaacaac ggattctaga cgtatcggtc
     7021 ttgtaggtga atactctctt gaatacaatg cttcaaactc tggggcaatt ttaaatttaa
     7081 agtctgaata aaagggagtt agtactccct tttttgatag gaggaattac agtggtaaaa
     7141 aaagatgaaa ctaaaaaaga tgaagtcgtg aaatatagag taggtaaaac taaaaatttt
     7201 gttgggtttg ttcatcctaa aactcgtaga tttatcacag cagattcaaa taacgaattt
     7261 atcatttcta tagatgataa agaagcaatt gcaattttgg aagatgcaat tgatgttaat
     7321 gaaatttagg aagtgatctg atggatgaat cgctaaaaac ggaaatcatt gagtctacaa
     7381 aagaagattt tccagatttg agtgaagaac gcataactaa tttattagaa ataattttgc
     7441 tagaaattga atcatacaac acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg
     7501 tgattagtga agtgttgtat aaaataataa aaaatgaatc agaaaaaaca gtatcttcag
     7561 ttagacgtgg cgatacaacg attagttatg cttcaacaac aaatgatgtt agtgagttac
     7621 ttctaggcta tggtgatttg atacgaagag ttattggttg tggaggattg gagttttttt
     7681 aatgaatgaa gcagatattt tagaaactac ttatgaagat agttgtatta ttgaaagact
     7741 gacggacatt gaagattcta atacaaatat tactattcaa gattacaaaa aagtatatga
     7801 taatcctatt tcttgcgctc tttcacaagg tcaaatcgat ggactagcag tcatagaaga
     7861 tggagagatg gtaaatgttt caactgacac atataaatta tttgttcatc ctaagattaa
     7921 actaaaaaaa ggagatcgaa taacaataac tcaaaaggcc agtggcttaa ttttttctct
     7981 atttgctact aagccttttt actatcctag tcattgtgaa gtgaatttga taggaagtga
     8041 gaaaaatggg taatctcaaa tatgaatcaa atgcagaaaa gatcattgag aattttaaaa
     8101 atatgactgt aattgctcaa aaagaaggaa tatccttcgt taacgattca atgaataaag
     8161 tcgttagttt agctaaacca ttaactcctg taaaatcagg taatttaagg cgaggatatc
     8221 gtgtagtaaa agctagaaaa ctatcaatcg gtcgaattgt tggagcagtt ataaacaatg
     8281 aacattattt taaatatgta gaagaaggtc ataggactaa aaatggtggc tttgtcaaag
     8341 ggaaattcat gttaactcgt gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat
     8401 ttaaacaaat ggcaataaaa atcgttaaga aaggaaagta acatgaaaga tgaaatcatt
     8461 gctgcaatca gtagcaaatt aaaagaaatc tatcctgatg ggacaatata tcttgattca
     8521 gttatgcagt caactaaaga tttttatttc gttttatcag taatggaatc tggaactgaa
     8581 aatgtaggaa ttgatattca aaatgtttct ttcttaattg atattgcatt gattgataat
     8641 aaacctaata gaaatttaat taatgaatta gtctcacgtt gtgggacttt ttttaatacg
     8701 attacaatcg atgagcaaat attatttcca aaagcctatt tacctgatga agcagatggt
     8761 gttcaacata ttcgttttac attagaattt ccacaatata ttgaatggag tgaaagataa
     8821 atgggagaaa aaagaagtaa aaccggaatt atttctgtcg aaaaacctac ttggtttcct
     8881 ttggaagatg agacagggaa tttcccggta tatggtactg cgttgccaat gggaacagca
     8941 gtaagtatca aaccaacagc taattatgaa acaacacaag attatgggga ttcagtggtt
     9001 caagatcaat ttacagcttt tggtggtgca gaagttgaac tggaagcaaa tggatatact
     9061 cataaagttt tatctacaat tactggaggg aaaattgtta aaggtggtgc cttgcgttct
     9121 ggagaagata ttgcgcaaga tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa
     9181 taccgttata cggtctttta taaaggacag tttgctctag attcagatga aacgtcaacg
     9241 attgaaggaa gtaaagtaag ttttactcat ccaacttgga agggttcatt tgtagatgta
     9301 ccaggacttg gatatatgta ttcagttgat gaagatgatg aaggcgtgga taaagcaatg
     9361 atcggaaatt ggtttacaaa agtagcaatt ccaattgaag aagcagaact ttcaggaggt
     9421 acagaataat gtcaaaatat caaacaacga ttaaattaac gaaaaaagat gaagaaggta
     9481 aatatgagca agtacaattt aaatctgctg aatttttacc aggaacagtt gtagaagatg
     9541 ctgcaggagt tatggaagaa atgcaaacag cgactgataa acaatcagtt aaaaaagctt
     9601 taagtcgtgc ttattcattt attgcagata ctttatttga gggacaattt actggtgaag
     9661 attattgcaa aggaattgat gctcgtgaga ttgcctcatt gacaggaaaa ttattgaagt
     9721 ctgttactgc aggttttgat gaaacttata cagagacgaa aaaaaagtaa gtgaggcgct
     9781 caagtcacct tcatttaagt attcgattac ttaccgagaa ttagatataa agacgcaatt
     9841 actcgaagca ggttggacgt taccagaaat tgaacgtact gacttggatg agttaatgcg
     9901 tctttttgct tttagagatg cggtaaaaga gcatgaagat gttgagtact atgataattt
     9961 cactcaattt taggaggtga tactttgaat aacgatgacc taattctgaa gatgatacta
    10021 gatgaatctg gttttactgc cggtatgaat aatgctgtca aaaagttgaa ctcttttgat
    10081 gaaacaatcg aaagaaaaag tagaaatagt ggcagttcat taggtagtat ttggaaaata
    10141 tttgctggaa gttttctagc tagcggagta actagaatcg taggagctgg ctttgatcta
    10201 atcaagggtt ccataagtgg agcggttgat cgagtagata cgatgaataa cgctctacga
    10261 aatttccaaa atatgggatt tagtaattca gaaattatga agaatatcgg aaagaatggg
    10321 cttttatctc aaggtattca aggacttcct actgctttga atgatgcgat aagtcatgtt
    10381 caacttcttg cttcttctac aggtgattta actcgttcaa ctcaaatatt taaagcttta
    10441 aatgatggaa ttcttggttt cggtggttca actgatcaag ttaatgaagc tgttattcaa
    10501 ttatctcaaa gtttctcaaa cggaaaagta gacgcacaaa cttggaactc aatgatcaac
    10561 gctcaacttg gacctactct ttctgctatt gctaaaaaga tgggaattac aatgggagat
    10621 ctgaaagaag gtttatctca aggtaagatt tctgttgaag aattccaaaa tcaattaata
    10681 gaaatggata ccaaaggtgg cggaggactt aaatcattaa gtcaaatcgc taaagattct
    10741 actaagggaa ttaaaacctc tatacaaaat gcaaaaacag ctgttacacg tggtgttggt
    10801 gaggtaatag aaggattaaa taaagcatta gtagattcag atttaggtgg atttaaggga
    10861 attattgata aagtgggcag ttcaatggaa tcgttcttaa aagtaattgc tgcaaatatt
    10921 cctacagcag tatcattttt aagtaattta tttaacgaaa ttcaaaaatt tggttctgct
    10981 ttgaaattca tgatgccctt tcttgttcct gcagctaccg cttttggagc gttcatgttt
    11041 caacttaaag gtataccagc aataataaaa agcttcaata attttaagaa tgccataatt
    11101 ggcgttggaa attcattaaa gattatgggg gcaatagctg ctgctaattc atttgtttta
    11161 attattggtg cagtcgttgg tgcaatagct gttttcggtt actttatggc aaccaatgaa
    11221 gagtttagaa acaaggttat atctatttgg aatgatgtga aagattccgt aattggtgta
    11281 ttaaaaaata taaaggactg gggaattgat acttggaatt ctgctaaaga aatggcatca
    11341 aatgctgttg agagtgtcaa ggatgcttgg tctggaataa aggaatggtt ttcaaatact
    11401 tggcaaggca ttaaaaatgg agcaacaggt ttatttgata aaacagtaga aacatctatg
    11461 aatgcggttg atagtgtaaa aaacgcatgg tcaaatacaa agcaatggtt ttctgatatt
    11521 tggcagagca taaaagattc agcaactgaa aaatggaatg aaattaaagg ttctatcatg
    11581 gaagttgctg gtccattgat tacaggttta aagaacgcat tcttgcatgt cactttttat
    11641 ttagagacct tatggaataa tctagttgag attggtaaaa atgtttttga gattttaaaa
    11701 aatgttatac ttacgccagt tttgtttatt acctccatga tttctggtgg atgggaagaa
    11761 acaaaaaaca acatgattgg tgtatggaat aatattaaag aaagtgctct aaatatttgg
    11821 gagtctataa aaaatatttt tgttagttac attacaaata tttattttgc tgcgcttaat
    11881 atttggacag ggttcaaact tactttgata aacatttgga atgaagtagt aagtcaagct
    11941 aaatcaattt ggattaatgt aaaatacttt tttattaatc tttggattga cattaagtat
    12001 tttgctattc aaaaatggat tgaattaaaa ttcgggatta ttcaaacttg gattgattta
    12061 aaatataatg ctattactac ttggaacaat attaaacagt tcttcaaaga tacttggcag
    12121 aacattaaag atatagcata caatacatgg atttctataa agaattccat gatcaatact
    12181 tggaataaca tcaaggaatc tttctggaat attgttactg gaattgttaa ctccgctgaa
    12241 aatgcatgga ctaatctaaa aaatggtgtt tcaaaagcaa ttaatcgggt gaaagaaatc
    12301 tttgattcat taagggaaat caatttattc gaaatcggta agaatatcat tgatggactt
    12361 atcaatggtg tagtagaaaa atggaatgca ttgaaaaaga ctattaaagg aatagctgga
    12421 agtatcaaag attctattaa gggtgcatta ggtattcatt ctccatctag atggatgagg
    12481 gatatggtag gtaaaaatat tgttcaaggt attatcgttg gtattgataa agaaaaaagt
    12541 aaattggatc agacaatgac tgatttagtg aaaactccgt cggttcaacc agttattaca
    12601 gggtctaata gtcaaccagt ggtacaagct aaacaaaata catcttcaaa tgcagtgaat
    12661 gaaattcact tgcatttaaa tgtttatgga gatctacctg attcgatgat taaacaaatc
    12721 gccaagaaga tgaaaacaga attgacgaga caaatgaaac gagatgctga tgcagtagga
    12781 gggacattat atgcaactta aaagaggaca attttttatc aatcaacatt attcttctga
    12841 gtttaacgta tatattcaaa ataggcctgc ctctgtttca gctagtcgtg taattgaatt
    12901 gagagaacgt gaaggcaatg actcaattat tatcgataaa gcctattaca aaaacgttac
    12961 tagaaaaatt gaatgttatt acaaagcacc atcgattgat ttagtgcagg aatgggaaga
    13021 tcgaattact gaatggttag atatgaactc ttacagtgat tttattcttt attacgatca
    13081 acaatatatc tatcaagcta tagtaaccga agcgccagaa tttaaaggaa caagaaaaac
    13141 agggaacata gttccatttg aatttacagt tagtcttcga ccattcaaag aaaattatag
    13201 tggtcgtttt gctattcaac agataaaagc tttcgagtta tttaatccag agaagtatgc
    13261 ttcaaaaccg cttattaaat tgagtggttc tggagatgct tctttttata ttaataatga
    13321 taaatatgat ttgaaactat tagatagaga attatatata gattctaaac tagaagagtc
    13381 ttatcgaaag ctagatgaca atttagaaca tcaagatcat gtcactttat ttttagattt
    13441 tccatttcta taccctggaa aaaatgaaat caaatggacg aataacatac attcgtttga
    13501 gataatacca aggtggtgga gaaaagtatg ataccaagaa tatatagtcc taccgagaca
    13561 gatttttcta cgaatggttt agggattttg aaggacacca caaaatgtga gatatatgaa
    13621 gtagcaaatg gaaaatatga attagagttg gagtatccat taggtactcg atttgatgaa
    13681 tattttgaaa atgactatca aataaaagca aagtcaaatg atcaagaaga gtatcatatc
    13741 ttttttattg atgataaaga tattgatacc tttttagata ccgtaactat ttatgcccag
    13801 agtcgtacaa atcggcttgg aagacgagcg gtcacttttg ctggagtcga ctctaaaact
    13861 ggtcgagaag caatggcaat tattgaaaac aatatggata aaaaatcaga tatacgctta
    13921 tattctgata ttacaactgt atcaagtaca acttttgaag caagaaatgt tttaaattgt
    13981 attgctggtg aacaaggatc tttacttcag tattggggcg gagaaattaa acgtgagcct
    14041 tttaagctat ctttattaaa gcgaagaggt cgtgataata ttggcacaat ccgatatgga
    14101 aaagatttat ctgggttaaa agttaagtta gattggacag gcgtgaaaac aagaattatt
    14161 ccgtatgctg atcctcaaag tgatgcaggt acaactagcc gaatttatgg ctcgccagta
    14221 gatagcgaat atattaataa ttatcctgat gtatatacag aacacgttca attcacagaa
    14281 gaacagggag ttaaagacgt taatagctta aataaaatag ctaagaatta tttcaaaaca
    14341 attaatccag gctgtgataa accgaagatt tctattaccg ttgagttcga taaattaacg
    14401 gacaccgaag aaggaaaaga attcgctaag ataagaaatt atgggttatt tgatacgttt
    14461 aaaatatatc atcgaaaata taaactatat tttgaatcga aagttagcgg tgttcaatat
    14521 gattcattat ctgaaaaggt tttgaaattg gaagctgggg atgctcaagt tgctttttat
    14581 caacaacaag ctgttactat tcaagataaa ttaaaagatt atgctacgaa taattatatg
    14641 agtagtttta atgattatgt gtcctctatg attgcaggtc aaggaaatgc aggcggttat
    14701 gtagttttat ggccaaaaga aaaaccttca aatattttta taatggatag ccctgattta
    14761 aacaaggcaa aagaagtatt aagaatgaac aaaaatggaa ttgccttttc taaaaatggt
    14821 tggaatggtc cttttaattc cgcttggaca ttggatagta tattcaatgc taattttatt
    14881 caaacaggat taataaaggc agatatcttt caaaactctt ttaataaaac tggagatgta
    14941 ttgaaattag ttaatggact acttcaaatt tggaataaca aaaagaaaat aatggagtta
    15001 accaaaaaag gaatggagtt ttggaattct aatagttcaa ttggaacgat tggaacaact
    15061 gattctgctg gtaatccttt tcctggagct tctactccca cacctattga agataattct
    15121 ttagttattc gtacaaatgg agacggcaaa tatattttga tttctcctaa agttggtaaa
    15181 ggattagttt tattaggaaa tggtaaagca atttattttg gagacttaga tgtacaaggc
    15241 aaactcacag ttaacggaaa agaaatcaca gggaacaaca gtggaggaag tgatcctgga
    15301 actatccctc ctcaattgac gacggaagct gaaaaaagag catggaaaat ttggacaatg
    15361 ctgaaagctc gtggctattc tgaatatgca gctgcaggta ttctaggaaa tattcagggg
    15421 gaggttggag caagtatgaa ccctgataca gaacaacttg gtggtccagc ttatgggatt
    15481 gttcaatggg atggttctgc ttatccgtta gtcggatcac caacttggaa cggacgagag
    15541 tatgttcagc gtttgatgaa caccgcaggg attcaagaag attatcgaag tattgaagcc
    15601 caagtaaaat tattagattg gtgtatgttc aacggtcaat ggctcggaaa agtaaatcca
    15661 accacagtat caggatttaa atcgatcaat gatgccaaaa gtgcagcgta tgcttttgaa
    15721 atgaacttcg aacgcccagc ttctgcacat ccagaacgcc aaaattatgc ccaatcttgg
    15781 tataacaaat tacatggatt aactagtcca gaacctggag ggaatttcat ttgtccaatt
    15841 caaaaaccag tgacagttac ttcagaatgt ggatggagaa ctagtccaat aaatggcggc
    15901 caagaatttc ataatggaat tgatcttgta aatggaaatc ctaatacacc tgtttttgca
    15961 gcattagatg gggaagttgt tcaggctggt gctaattatt atgactggta tggtaattac
    16021 gtggttatta aacataataa tgggaagtgg acaggttatg ctcatttgtc tcgtattgat
    16081 gtttctgttg gacaaaaagt ccagaaaggt gctcaaatag gcttgatggg aacaactggt
    16141 ccatctacag gagaacatct acattttcaa attatgaaaa attattggcc acagccagtt
    16201 gttgattttg agaatccaag aaattatatc caattttaag gtggtgattc tatgagtaaa
    16261 tggaatgtcg ttttaagtac aacagaacca tataattatg tggggatgat tcaagttcga
    16321 caaggcaata agaatacaga ggttatggaa gcgactatag ttgaaaatgg tcttccctac
    16381 gatttatcag aatgtaaggt atattttgaa tcagttgtag gtgggaaata tccagtccaa
    16441 ttagaaacaa aaattgtgga tgctaaaaaa gggaaaatta a
//
LOCUS       DASHWH010000062        16446 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.065, whole genome shotgun sequence.
ACCESSION   DASHWH010000062 DASHWH010000000
VERSION     DASHWH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.065"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..615)
                     /locus_tag="U2R41_002101"
     CDS             complement(<1..615)
                     /locus_tag="U2R41_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8889492.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCK"
     gene            complement(863..1699)
                     /locus_tag="U2R41_002102"
     CDS             complement(863..1699)
                     /locus_tag="U2R41_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEM8889493.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1710..2639)
                     /locus_tag="U2R41_002103"
     CDS             complement(1710..2639)
                     /locus_tag="U2R41_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEM8889494.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2831..3184
                     /locus_tag="U2R41_002104"
     CDS             2831..3184
                     /locus_tag="U2R41_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM8889495.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3258..3914)
                     /locus_tag="U2R41_002105"
     CDS             complement(3258..3914)
                     /locus_tag="U2R41_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM8889496.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4140..6074
                     /locus_tag="U2R41_002106"
     CDS             4140..6074
                     /locus_tag="U2R41_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8889497.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6160..7383)
                     /locus_tag="U2R41_002107"
     CDS             complement(6160..7383)
                     /locus_tag="U2R41_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8889498.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7377..9275)
                     /locus_tag="U2R41_002108"
     CDS             complement(7377..9275)
                     /locus_tag="U2R41_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEM8889499.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9269..10387)
                     /locus_tag="U2R41_002109"
     CDS             complement(9269..10387)
                     /locus_tag="U2R41_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8889500.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10470..11303)
                     /locus_tag="U2R41_002110"
     CDS             complement(10470..11303)
                     /locus_tag="U2R41_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8889501.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11296..12213)
                     /locus_tag="U2R41_002111"
     CDS             complement(11296..12213)
                     /locus_tag="U2R41_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8889502.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12440..13564)
                     /locus_tag="U2R41_002112"
     CDS             complement(12440..13564)
                     /locus_tag="U2R41_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8889503.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13747..14655
                     /locus_tag="U2R41_002113"
     CDS             13747..14655
                     /locus_tag="U2R41_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889504.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14796..16409
                     /locus_tag="U2R41_002114"
     CDS             14796..16409
                     /locus_tag="U2R41_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8889505.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5195 a         3226 c         2726 g         5299 t
ORIGIN      
        1 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
       61 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
      121 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
      181 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
      241 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
      301 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
      361 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
      421 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
      481 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
      541 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
      601 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
      661 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
      721 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
      781 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
      841 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
      901 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
      961 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
     1021 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
     1081 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
     1141 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
     1201 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
     1261 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
     1321 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
     1381 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
     1441 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
     1501 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
     1561 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
     1621 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
     1681 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
     1741 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
     1801 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
     1861 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
     1921 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
     1981 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
     2041 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
     2101 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
     2161 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
     2221 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
     2281 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
     2341 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
     2401 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
     2461 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
     2521 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
     2581 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
     2641 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
     2701 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
     2761 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
     2821 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
     2881 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
     2941 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
     3001 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
     3061 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
     3121 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
     3181 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
     3241 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
     3301 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
     3361 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
     3421 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
     3481 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
     3541 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
     3601 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
     3661 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
     3721 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
     3781 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
     3841 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
     3901 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
     3961 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
     4021 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
     4081 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
     4141 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
     4201 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
     4261 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
     4321 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
     4381 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
     4441 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
     4501 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
     4561 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
     4621 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
     4681 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
     4741 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
     4801 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
     4861 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
     4921 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
     4981 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
     5041 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
     5101 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
     5161 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
     5221 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
     5281 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
     5341 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
     5401 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
     5461 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
     5521 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
     5581 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
     5641 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
     5701 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
     5761 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
     5821 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
     5881 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
     5941 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
     6001 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
     6061 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
     6121 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
     6181 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
     6241 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
     6301 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
     6361 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
     6421 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
     6481 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
     6541 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
     6601 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
     6661 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
     6721 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
     6781 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
     6841 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
     6901 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
     6961 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
     7021 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
     7081 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
     7141 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
     7201 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
     7261 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
     7321 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
     7381 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
     7441 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
     7501 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
     7561 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
     7621 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
     7681 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
     7741 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
     7801 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
     7861 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
     7921 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
     7981 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
     8041 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
     8101 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
     8161 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
     8221 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
     8281 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
     8341 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
     8401 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
     8461 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
     8521 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
     8581 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
     8641 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
     8701 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
     8761 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
     8821 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
     8881 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
     8941 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
     9001 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
     9061 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
     9121 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
     9181 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
     9241 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
     9301 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
     9361 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
     9421 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
     9481 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
     9541 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
     9601 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
     9661 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
     9721 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
     9781 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
     9841 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
     9901 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
     9961 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    10021 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    10081 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    10141 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    10201 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    10261 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    10321 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    10381 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    10441 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    10501 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    10561 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    10621 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    10681 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    10741 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    10801 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    10861 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    10921 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    10981 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    11041 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    11101 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    11161 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    11221 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    11281 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    11341 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    11401 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    11461 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    11521 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    11581 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    11641 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    11701 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    11761 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    11821 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    11881 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    11941 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    12001 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    12061 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    12121 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    12181 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    12241 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    12301 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    12361 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    12421 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    12481 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    12541 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    12601 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    12661 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    12721 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    12781 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    12841 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    12901 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    12961 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    13021 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    13081 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    13141 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    13201 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    13261 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    13321 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    13381 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    13441 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    13501 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    13561 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    13621 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    13681 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    13741 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    13801 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    13861 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    13921 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    13981 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    14041 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    14101 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    14161 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    14221 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    14281 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    14341 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    14401 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    14461 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    14521 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    14581 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    14641 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    14701 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    14761 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    14821 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    14881 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    14941 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    15001 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    15061 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    15121 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    15181 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    15241 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    15301 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    15361 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    15421 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    15481 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    15541 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    15601 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    15661 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    15721 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    15781 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    15841 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    15901 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    15961 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    16021 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    16081 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    16141 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    16201 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    16261 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    16321 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    16381 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    16441 ggagtt
//
LOCUS       DASHWH010000063        15309 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.066, whole genome shotgun sequence.
ACCESSION   DASHWH010000063 DASHWH010000000
VERSION     DASHWH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.066"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            278..787
                     /locus_tag="U2R41_002115"
     CDS             278..787
                     /locus_tag="U2R41_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8889506.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U2R41_002116"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U2R41_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8889507.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U2R41_002117"
     CDS             2322..3302
                     /locus_tag="U2R41_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8889508.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U2R41_002118"
     CDS             3424..4842
                     /locus_tag="U2R41_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEM8889509.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6381
                     /locus_tag="U2R41_002119"
                     /pseudo
     CDS             4913..6381
                     /locus_tag="U2R41_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            6391..6858
                     /locus_tag="U2R41_002120"
     CDS             6391..6858
                     /locus_tag="U2R41_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8889510.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /locus_tag="U2R41_002121"
     CDS             6894..7766
                     /locus_tag="U2R41_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8889511.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="U2R41_002122"
     CDS             7848..10382
                     /locus_tag="U2R41_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8889512.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="U2R41_002123"
     CDS             10538..11311
                     /locus_tag="U2R41_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889513.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="U2R41_002124"
     CDS             11304..12095
                     /locus_tag="U2R41_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8889514.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="U2R41_002125"
     CDS             12105..12596
                     /locus_tag="U2R41_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889515.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="U2R41_002126"
     CDS             12614..13024
                     /locus_tag="U2R41_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8889516.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="U2R41_002127"
     CDS             13028..14428
                     /locus_tag="U2R41_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEM8889517.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="U2R41_002128"
     CDS             14451..14825
                     /locus_tag="U2R41_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8889518.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5314 a         2155 c         3060 g         4780 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat tacatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat cttttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacaa ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacctacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag agtaagcgcc
    15241 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
    15301 ctggaggtt
//
LOCUS       DASHWH010000064        14835 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.067, whole genome shotgun sequence.
ACCESSION   DASHWH010000064 DASHWH010000000
VERSION     DASHWH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.067"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            413..1027
                     /locus_tag="U2R41_002129"
     CDS             413..1027
                     /locus_tag="U2R41_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889519.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1800..3539
                     /gene="yfcC"
                     /locus_tag="U2R41_002130"
     CDS             1800..3539
                     /gene="yfcC"
                     /locus_tag="U2R41_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEM8889520.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3696..4880
                     /gene="iadA"
                     /locus_tag="U2R41_002131"
     CDS             3696..4880
                     /gene="iadA"
                     /locus_tag="U2R41_002131"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEM8889521.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4991..5194
                     /locus_tag="U2R41_002132"
     CDS             4991..5194
                     /locus_tag="U2R41_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM8889522.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5295..5957)
                     /locus_tag="U2R41_002133"
     CDS             complement(5295..5957)
                     /locus_tag="U2R41_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889523.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5970..7472)
                     /locus_tag="U2R41_002134"
     CDS             complement(5970..7472)
                     /locus_tag="U2R41_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889524.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8020..9699
                     /locus_tag="U2R41_002135"
     CDS             8020..9699
                     /locus_tag="U2R41_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM8889525.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9826..10305
                     /locus_tag="U2R41_002136"
     CDS             9826..10305
                     /locus_tag="U2R41_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEM8889526.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10524..11204
                     /locus_tag="U2R41_002137"
     CDS             10524..11204
                     /locus_tag="U2R41_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEM8889527.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11212..12543
                     /locus_tag="U2R41_002138"
     CDS             11212..12543
                     /locus_tag="U2R41_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8889528.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12558..13520
                     /locus_tag="U2R41_002139"
     CDS             12558..13520
                     /locus_tag="U2R41_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEM8889529.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13631..13969
                     /locus_tag="U2R41_002140"
     CDS             13631..13969
                     /locus_tag="U2R41_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEM8889530.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14048..14755
                     /locus_tag="U2R41_002141"
     CDS             14048..14755
                     /locus_tag="U2R41_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEM8889531.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3192 g         4303 t
ORIGIN      
        1 ttgtattttc ttgcttgcac ttggataaga gattacttac aataaattca gatagaaaca
       61 gctgaataaa aaaaggctcc ctgaccctta gttggcgcta agaatcaggg aatgaccagt
      121 cacacacata caccctgacc gatcattttg ccatagtcaa tgcagtgcat cgacctaatt
      181 tattgtacct aatctagggt gaaaattcta cccctatgta gatattttcc atggtctgca
      241 cgttgtttta tttgctatgt caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt
      301 tgaaacagca aagaccacat gtgttactgt ctaactactg tttttttatg tacttcctta
      361 gattaggcag ctgtttctat tcatgtcaag tatagctagg cgggagagat aaatgagttt
      421 atgttatttt gactattatc accaattgga agagaaatta aggatagaag agccgctgat
      481 gcttcaagct ggcacttgga tcgcgaaaca aattaatcaa ggtggtagat tgcagatata
      541 tgctagccgc acactaagtg gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt
      601 accaagtata ttgattgaaa atccagccgg aggtgtctat gaaacgctag aaggtgcagg
      661 acaggcaatt attgatcagc tttctgtcaa atcagaagac atttttcttc ttctttcgaa
      721 tgaaggaaga aacccggcaa tcatcgaact agctcagtgg atcaaagaaa atggtcatcc
      781 attaatcatt gttactggat ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg
      841 tttgcgatta tttgaatttg ctgatttggt tttggacaac cacgccaatt tacatgatgc
      901 agtcttgact ttgccaaatc tagatccagc gatctgcgga acagcctctc ttgcaacgat
      961 ctttttgctg cagcaagttt tgtattttac agcaaaacag ctagtccagg acacaagtgg
     1021 aaaatagtta tccacatgtt gacaaagttc cttaaaaaaa taatgttcca cataaaaatg
     1081 gagcgtttga aaaaaagaaa acgattatta atgaggatta acaacgacta ctttttttgt
     1141 tttagttatt ttaagatgga tcatctttga ttttaagctt gttttgttta aagtttgtta
     1201 tttttataag ttgtttcaca gttatgagaa atgagttata agaatatgta gtgtcagaaa
     1261 aataaaaagt agtggaaaaa gagacaagaa agaatccata tggattcttt cttgtctctt
     1321 aatacacgtt gatgaaagag tcttccccaa aactctttac aaagtaatta tagtacattt
     1381 gttatcatta ttgcaatgaa gtttacctga tataacaaaa ttgtccaaca ggataccttt
     1441 ttctaataga aataaaatac acaaaaattg aaggatagat aatggatatt tataagtgtt
     1501 tattattttt tttgcaatga aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga
     1561 cgagattttc tctaatctaa aaataaaaaa agaattttaa ggaaaaacag agaaataaaa
     1621 tacttaatat aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata
     1681 gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc
     1741 atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt
     1801 tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc
     1861 atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc
     1921 cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta
     1981 ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg
     2041 tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc
     2101 cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac
     2161 tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg
     2221 atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct
     2281 ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg
     2341 tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg
     2401 gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag
     2461 ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag
     2521 aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga
     2581 tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga
     2641 gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga
     2701 cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat
     2761 atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt
     2821 tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat
     2881 tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag
     2941 gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca
     3001 ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat
     3061 ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc
     3121 ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga
     3181 tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc
     3241 aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa
     3301 tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc
     3361 agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc
     3421 taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat
     3481 tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt
     3541 actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc
     3601 attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct
     3661 atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag
     3721 tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg
     3781 ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg
     3841 agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag
     3901 gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg
     3961 gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt
     4021 tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt
     4081 cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg
     4141 ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt
     4201 ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag
     4261 ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa
     4321 tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac
     4381 cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg
     4441 ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac
     4501 tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac
     4561 aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg
     4621 ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa
     4681 tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa
     4741 aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg
     4801 atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga
     4861 ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct
     4921 gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg
     4981 aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg
     5041 tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa
     5101 agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa
     5161 agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa
     5221 gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat
     5281 cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact
     5341 tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt
     5401 tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt
     5461 ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg
     5521 actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca
     5581 attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta
     5641 gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa
     5701 gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt
     5761 gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa
     5821 agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg
     5881 aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat
     5941 tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat
     6001 cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg
     6061 gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa
     6121 acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt
     6181 ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc
     6241 tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca
     6301 actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta
     6361 cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta
     6421 atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt
     6481 ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt
     6541 gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc
     6601 attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc
     6661 ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag
     6721 ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc
     6781 atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc
     6841 aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg
     6901 tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt
     6961 gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc
     7021 attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca
     7081 gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa
     7141 taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta
     7201 agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt
     7261 tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct
     7321 ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca
     7381 aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt
     7441 gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt
     7501 tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa
     7561 catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga
     7621 aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta
     7681 gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt
     7741 tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt attctggcta
     7801 tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc
     7861 aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat
     7921 agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga
     7981 catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc
     8041 ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat
     8101 atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc
     8161 gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta
     8221 acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta
     8281 ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa
     8341 gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt
     8401 gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat
     8461 ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct
     8521 atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta
     8581 gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa
     8641 atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt
     8701 gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga
     8761 aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa
     8821 aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat
     8881 ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa
     8941 aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc
     9001 actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat
     9061 cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat
     9121 cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa
     9181 ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac
     9241 aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt
     9301 gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc
     9361 ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt
     9421 gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga
     9481 ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca
     9541 atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc
     9601 cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca
     9661 gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc
     9721 tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag
     9781 catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca
     9841 aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat
     9901 gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata
     9961 tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg
    10021 aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt
    10081 cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt
    10141 ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa
    10201 gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat
    10261 ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa
    10321 gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct
    10381 gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt
    10441 agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac
    10501 agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa
    10561 ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga
    10621 cagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg
    10681 accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg
    10741 ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg
    10801 gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct
    10861 atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag
    10921 aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg
    10981 cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc
    11041 cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt
    11101 acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata
    11161 gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca
    11221 aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta
    11281 agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat
    11341 gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc
    11401 aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg
    11461 gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg
    11521 atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac
    11581 cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga
    11641 ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc
    11701 acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg
    11761 atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat
    11821 ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg
    11881 cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt
    11941 ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta
    12001 aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc
    12061 atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg
    12121 aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata
    12181 tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag
    12241 ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta
    12301 cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat
    12361 atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta
    12421 gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg
    12481 tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct
    12541 tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg
    12601 ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag
    12661 gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa
    12721 cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag
    12781 gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc
    12841 agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc
    12901 ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga
    12961 tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg
    13021 tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt
    13081 cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc
    13141 cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg
    13201 gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag
    13261 agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt
    13321 ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca
    13381 atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa
    13441 aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata
    13501 agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca
    13561 aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag
    13621 gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga
    13681 tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac
    13741 tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga
    13801 tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg
    13861 aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga
    13921 aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa
    13981 ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga
    14041 aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat
    14101 tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata
    14161 cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca
    14221 aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta
    14281 caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc
    14341 tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt
    14401 cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt
    14461 ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat
    14521 tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc
    14581 gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc
    14641 gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac
    14701 attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa
    14761 agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac
    14821 catttatttg gaata
//
LOCUS       DASHWH010000065        14700 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.068, whole genome shotgun sequence.
ACCESSION   DASHWH010000065 DASHWH010000000
VERSION     DASHWH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.068"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(200..1456)
                     /locus_tag="U2R41_002142"
     CDS             complement(200..1456)
                     /locus_tag="U2R41_002142"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEM8889532.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1568..1773)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2036..3337
                     /locus_tag="U2R41_002143"
     CDS             2036..3337
                     /locus_tag="U2R41_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8889533.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3406..4005)
                     /locus_tag="U2R41_002144"
     CDS             complement(3406..4005)
                     /locus_tag="U2R41_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8889534.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4259..5140
                     /locus_tag="U2R41_002145"
     CDS             4259..5140
                     /locus_tag="U2R41_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889535.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5153..6763
                     /locus_tag="U2R41_002146"
     CDS             5153..6763
                     /locus_tag="U2R41_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEM8889536.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6840..7499)
                     /locus_tag="U2R41_002147"
     CDS             complement(6840..7499)
                     /locus_tag="U2R41_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8889537.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7782..8447)
                     /locus_tag="U2R41_002148"
     CDS             complement(7782..8447)
                     /locus_tag="U2R41_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEM8889538.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8594..9199)
                     /gene="dhaL"
                     /locus_tag="U2R41_002149"
     CDS             complement(8594..9199)
                     /gene="dhaL"
                     /locus_tag="U2R41_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEM8889539.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9227..10237)
                     /gene="dhaK"
                     /locus_tag="U2R41_002150"
     CDS             complement(9227..10237)
                     /gene="dhaK"
                     /locus_tag="U2R41_002150"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEM8889540.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(10212..10658)
                     /gene="dhaM"
                     /locus_tag="U2R41_002151"
     CDS             complement(10212..10658)
                     /gene="dhaM"
                     /locus_tag="U2R41_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEM8889541.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(10697..11656)
                     /locus_tag="U2R41_002152"
     CDS             complement(10697..11656)
                     /locus_tag="U2R41_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8889542.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            11826..12146
                     /locus_tag="U2R41_002153"
     CDS             11826..12146
                     /locus_tag="U2R41_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEM8889543.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(12258..13127)
                     /locus_tag="U2R41_002154"
     CDS             complement(12258..13127)
                     /locus_tag="U2R41_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8889544.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(13223..14527)
                     /locus_tag="U2R41_002155"
     CDS             complement(13223..14527)
                     /locus_tag="U2R41_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8889545.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(14524..14685)
                     /locus_tag="U2R41_002156"
     CDS             complement(14524..14685)
                     /locus_tag="U2R41_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889546.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT         4091 a         3000 c         2681 g         4928 t
ORIGIN      
        1 ttaatcatat tttaatatgt atcaattatc cgaattgttc acacttttga aataattaaa
       61 aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag attgtattaa cgacaaccag
      121 cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc cctttttcaa
      181 ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt ttcttacctt
      241 ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca aaatttaaat
      301 cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca cgtgcttgac
      361 gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca ggtgtgcttg
      421 aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca tttagttctt
      481 taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac
      541 tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt
      601 ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa gtaaagaatt
      661 tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag aactcaaatg
      721 gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg aatttcgttc
      781 cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct ggtccttcta
      841 attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct ccaccgattt
      901 gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc aggatttggt
      961 aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata tcttttgcta
     1021 acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt aaagagattt
     1081 ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca aacaattttc
     1141 tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt tgcaacacac
     1201 gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca atcagaatat
     1261 atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc aaatgtccga
     1321 tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa
     1381 cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt
     1441 catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc aggaacctgc
     1501 tagcatgtgt aattaattat ttcttcagga taggatagga aataaaaaaa gtcccttggc
     1561 atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta gatatccaac
     1621 atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt
     1681 aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa acattggctt
     1741 taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct atcatagagc
     1801 gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca ttttttcttt
     1861 tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag aaataacttt
     1921 ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt attctttctt
     1981 cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga ggtatatgga
     2041 aaaaaagcaa aaaattattt taggagccac ttctgcttta actatcgtta ttttaattct
     2101 aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa
     2161 aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta ctcaagcaaa
     2221 aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa aagatagtaa
     2281 gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga aagaacaact
     2341 gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct tgatcgtgga
     2401 cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata tggtgactgt
     2461 tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt tagttaattt
     2521 gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc ttgcgctacc
     2581 tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg aatatatgga
     2641 taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca aaaatgtcta
     2701 taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga tcgatgcctt
     2761 tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg
     2821 ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca atgaaaaaac
     2881 aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag acggctggag
     2941 ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag aagatgtaaa
     3001 aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa caaatattct
     3061 gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag aaaatcaaaa
     3121 atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg cttctacacc
     3181 agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata tcgatggcat
     3241 tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca tcaatgtcca
     3301 tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa
     3361 aaagccgaac tgacatttta tcgtcagttt cggctttttt atgctttatt tcttcttcgg
     3421 tgcgattccc ttgatcagga cttctgtcca ttctgctgta tatggaagca gttttgcgta
     3481 tatctccggc ttagaaattc cttctaccat tgcttgaaaa tagaggatca agaactcatc
     3541 cgaatatttc aaatccactt ttccttcttt ccgccctcga tggaaaagat caagcatgat
     3601 actgaaactt tcttgcgtat acgcatccat cgcgtcagaa aattcttttt tttctttttt
     3661 tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc actcgattga gataacggat
     3721 tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga agattttccg caatcaacgc
     3781 tttcgcttct tgccctaaat cattcataaa tgcaaaaaat acttcataaa tcagcccttc
     3841 cttgctgcca aaatatttaa aaatcgtcgc tttacttacc tgagcgttct cagctacctt
     3901 atccattgtc actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa
     3961 aatctctttc tttttctctt gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg
     4021 taattttaaa cttttataat atatatagtt caaatttatt ctgaggttta ttataactat
     4081 ctattgagaa agtatcaagc tagcaattcc ttgtttatga attcattgac agaaatttat
     4141 aaaaagaata ttatgtacta aaataataaa aaaagtttta taaaaaggta taaaaccttt
     4201 ttattaaact atttaatctt gtttagtata tatttttaga aaaaggagtg gggtttttat
     4261 ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa
     4321 agatgtctct ttcaccgtac attcaggtga agtcgttgga tttatcggac caaatggttc
     4381 tggaaaatcc acgacgattc ggacactttt agggattata aaaaaagacg gcggaacggc
     4441 aacgattttt gggcaagatg tatgggaaga tagtttatct atccatgaac gtctttctta
     4501 tgttccaggc gatatctctt tgtggggaaa tttgactgga ggagcgatca tcgatctctt
     4561 catgaagctt catggaaaag ggagtgctca aaaaaaagat gaactgattc aacgtttctc
     4621 tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt
     4681 gattgctgct ttggctgttg attcggatct ctatttattt gatgagccta cttctggttt
     4741 agatcctttg atggaaacga ttttccaaga agaagttgaa aaattgaaag aacagggcaa
     4801 atccatccta ctttcttctc atattctcag tgaagtcgag cgactagcgg ataaagtcgt
     4861 gatcatacgc gaaggaacac tcgatgaaga aggaacactc gatgaattgc gtcatttgac
     4921 tcgttcgacc atcactatag aaacagaagc agagaatagt cacttagcac aattgaacgg
     4981 tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt gctgctgata acgaagcttt
     5041 cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc
     5101 gactcttgag gatttattca tgagacatta tgaaggatga ggaggaagcg aaatgactga
     5161 taaattttca cgctggtcca cccttttttc tcaaaacctt agccgtgact ggaaaaagat
     5221 cctgatatgg ctagtcggtg tcactgcttt ttctggtggt tttgtccctg cgtttgaaga
     5281 attgacttcc ggacaaggat tagctggatt atacgaaacc ttgcaaaacc cggcaatgat
     5341 tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag tatactttag gagcgatgta
     5401 tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct gtgatctctg ttttacacgt
     5461 ggtgagccaa acgagaaaaa aagaagaact tggtctgatt gaacttattc gttcctttcg
     5521 agtcggacgt caagcaaact ctttagctgt catcgctgaa agtattctga tcaatttgct
     5581 tttagcaata tcgatcagct gtacgatgat ttttttccaa gcggattcga tcactgcaga
     5641 aggtgcttgg ctttttgggc tatctgtagg tagtatgggg attttgggga gtgccgttgc
     5701 actgtttgct gcacaactga tgccaagttc agccagttcg aatggtctgt ctttagcgtt
     5761 tttaggtgga ctttaccttc tacgagcaat aacagatgta gctgattcct ccctttcgat
     5821 gtggaatcct ttaggctggt cttatttgac ctatccattt acaactaaca actggattcc
     5881 tttgctttat ttgtttttgt tcagtctaat cttgataatc agttcatttg ttttagagga
     5941 acatcgagat ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg
     6001 ttctctttta tccttgcgtg gtttattatt ctatctaaat aaagggatga caatcagttg
     6061 gcttatcact tttggtttga tgggggcggc atacggctct atctacggga atatgcaaca
     6121 gtttttagaa agtaacgact taatgaaaca gatgtttatc caatctggcg tctcaattga
     6181 aaaaaacttt acaagcacga tcatggtcgt cttgatttgt ctagctgcta ttttaccaat
     6241 tgctagcatc aatcgtttat ttacagaaga aacccacgga cgtatgaatc agctgttcgc
     6301 aacaaaaatt tctaggaacc aactttattg gaccaatatg ttactagcta ttctatttgc
     6361 tatattggca atcctgatta gttctggtgc actagggctt tctgctttgg cttcgatgaa
     6421 ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc
     6481 cgttttagtg tttatcggaa ttagtggctt tctattcgga tggcttcctc gaatcggtaa
     6541 acttgcttat atctacttag cttactcctt tgcactcaat tactttggcg gaattttgga
     6601 tttaccagaa tggttttcca aaacagctgt ccctagctgg gttccacgga tgccaaacga
     6661 ttcttttgat ctttccgtgt tccttacgtt atcatgtatc agccttgcac tgttcgtcct
     6721 tggttctatt ggttataggc gaagagactt actagaagga taataaaaaa gcatgtacaa
     6781 tttcatgcta gcctccaaat ctgggtcaaa aatccaacat ttggaggtca tttttttatt
     6841 tacaagtctt ctaactgctc tttatcaatg atccagactt ctcgattctt tctttttaca
     6901 taaccattct cctctaataa tttaaatttt ctagataaag tttcgggggt cgtaccgata
     6961 aatgcagcaa gttctttcat tttcattggc aggaaaaagt gatccttccc tgtcgcttta
     7021 gctaagtcaa gcagatagac agcgactcgt tcttctactt tttccatcat caaaaaagtt
     7081 gtctgctgct gtacttttgt taatttctca acgttgattg ctaaaagttt cagactaagg
     7141 gcgggataat gcagtaagag ttccgagaaa tctttttgtc tcaatatgca aatttccgtt
     7201 ttttctagtg cttcgccaaa taaagactca tttggaacgc ctagcaactg gctctctccc
     7261 tcatatccgc ccggctctat tactcgtaaa agctgttcct ttccgctagc agataattga
     7321 tacactttca tactgccgga ggccacgatc actaattgag gttctccatt cggcgcaaga
     7381 atctgttctc ctttttccac atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct
     7441 tgctgtacta agtgattgaa caaaggaaca aatgaaacac ataaatgatc atgactcata
     7501 atctctcctc cttctaaaca gctatttcta ataaagaaac ataacttccc ttattccaac
     7561 tgataatttt cttttatcat agcataaacg tcaagcgaac cagataccag caaatagagt
     7621 ggatttcttt ttgtcccttg ctttattttc cttcttttga gaccttaagt aattgattcc
     7681 tatcaatttt ttaaatattt actttttgaa taatcaaaaa caaaccttca ctcgcaagcc
     7741 gagagtaagg tttgtttttc gcattgctta tttcgtgttg tctattgaaa tttttccgca
     7801 cctagctttt gatatagtgg tgctagttct tgtaaggttt cagcggttat ttgctcaacg
     7861 tgttcgattt cttgcggata gaagaatagt ttacccattt gaaaggttcg gtctctcatc
     7921 gcttttttca actcttcttg tttctttgta tcggacaaat agtcagacaa agctaagtgg
     7981 tcgtcaagtt catgttctgg ttgcggccat atgtaataat ccttgatatc gactgttttg
     8041 ctccactctt tcagtatcgc aagcaactgg ttatacgctg caatgctacc atatcgttct
     8101 tcgcttttat aatgttggta catcaaatag atttggtatt gtttttgatt caacaaaact
     8161 gctagacagg cattctcgtc ttgacgagat tcgcttcggt acgcgctcca aaaatgactg
     8221 cgtaaattcc agccattcgt ccagctttcg attttaggtt tgctcatgcc gtacatatca
     8281 ggcaaaagtg cagctgtctg gagctgaatc tctttccatt tttgccagtg ttctttatat
     8341 tctttcttga tttgttcaat cttatctgtt ggatggctct ctttgagctg tttgaatgcg
     8401 tagattggtc gatcaaacaa ctgataatct gattttccaa gttccattgt ttcgcctctt
     8461 tccgttatct tttgtattct acctgaattt tgcgatatgg acaaatagag gttgtgaaaa
     8521 agctgttctg catcaagtca taagaacagc caaacaaaaa actgcgatga gcatcaccgc
     8581 agttttattg aacttaaggt tgattgatca catctaaaag cgttgaaaat aaaatggcac
     8641 tagaagcagc tcctgggtcg atatgtccga ttgcacgctc tcctagatag gaagctcgtc
     8701 cttttttagc taccaagact ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta
     8761 aattccctgc cttcaatgcc tctgtgactg gcagccaaac atctaacatc gttttatcgt
     8821 cagcttctgc ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc acttcgccta
     8881 atttttcttg agaatcaata gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg
     8941 cagatccgta taacggacca gaagccccac cgactttcga tatcatcgcc atagagaata
     9001 ctttgaatgt atcgctgatt gtttcaggat gatcggcttg aaaagcttct tcataagctg
     9061 aaacccctct tcccatgtta tttccatggt ctccatcgcc aatcggggta tctagatcac
     9121 tgagatattg tttgttctga ttgattgcct ctgcaaattg cgtgagccat tcttggattt
     9181 cttgaacggt tagtttcata ttatcaccct tttatcttga gattttttac catgaaatcg
     9241 tctgtacatt gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta
     9301 gagaaagacc ttgcatatct agtgaggtca taaaattacc gacttttcgg aaaactacat
     9361 tgacgcctct ttcttccaac agagctaaaa catcattcat gaacacgaac tgttccatca
     9421 aaggcgtccc gcccattccg ttgactagta cacctgcttc ttttggtaaa ttcgagtaat
     9481 catctaaaat cttagtaaca agttcctttg ccagtacttt agaaggctgc atcttttctc
     9541 gacgatagcc tggctcaccg tggataccaa caccatactc gatctcatct tctcctagtt
     9601 cgaatcctgg tttccctact tctggtacag ttgctgctcg aagcgctaga ccgatcgtct
     9661 tagttgcctg tacgaccttt tctcccagtt ctttcaactc agctagggaa gcaccgtttc
     9721 ttgctgcatc gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg cgttttccag
     9781 cagtatacgt actattttca acggcgatat catcatctac gatcacactt tctacttcaa
     9841 tatcgtccat agcagctaat tcctttgcca tatcgaagtt caatgcatcc cctgtataat
     9901 tcttaacgat cagcaaaact ccttttccac tatcggcttc cttaatagcc gtctgaatct
     9961 gatctggagt tggagaagta aacacatcac caagcaccgc tgcacttaac atcccttccc
    10021 ctacaaaccc ggcatgggat ggttcatgtc cgcttcctcc accagaaacc aaaccaactt
    10081 gttccgcttt ctgattcttg gctacaacac gggaagattc tacacgatga acaagttctg
    10141 gatagctttt tactaatcct tccagcattt cttcgacaat attgcttgga tcattcatga
    10201 tttttttcaa tttattccca tcctttcttt ccggcatttt gtgcttctgt taagatttgc
    10261 tgagtatcat ctgttatacc ggcagtgatg gctgctgcaa aagctccttc cactaaaggc
    10321 gcatccacca gatgataacg ttgctgctgc tcttcttcca acatgtcttt tgccagttcc
    10381 gagttcaata ccgcgctacc gatatccgca aaaacaaaat aactagctgc atcgcttgat
    10441 tcattgactg cttctacgat tttcatcggg tctgatccga tctcgccatt tattccgccg
    10501 agagaaaaaa tttgcacctc ttcggatttt tgcatctgct caatcatttc tttaatacca
    10561 tctgttactt tttgactatg agaaataagc aaaatacttt ttttcacaag aatcttcctt
    10621 tcttttacat agttgctaac gattggtttc gctttcatta atgaacaatt caagtatata
    10681 ataaattttt cgtttgtcaa attaagctag acaaatcatc tttacctacg taactcaaaa
    10741 atacttccaa tggtgtgcga tagtgtaatg attttctagg aatatgattt cgttttgatg
    10801 ccaccgattg gataaaggtt tcggatacct tattaaaatc catagcttta tataagccat
    10861 ctttacgtaa taagccattc gaatgttcat ttagtcctcg ttgagaaggt gttcctggat
    10921 cagcgaaata aatcgaaata tcattggtat tactaatctt tttccaattc gagaactctt
    10981 ttccacaatc aaatgtgatg gatttaaaca agtgtttcgg tacagattga aaccattgat
    11041 tcagtctatt ctcaatatct atagcttgcc taccagcggg tttcaaggtg atgatcactt
    11101 tagataatcg ctcaactaac gtgatcaccg cacttttatg ttttccaccg acaatggtgt
    11161 caccttctag gtgaccaaac tcatgattga actgtatata atcagttgtt ctatcctgaa
    11221 tcgaacggcg aaaagcttgt tttcctcttt tttctttatg tccgtttggt tttcttttcc
    11281 ctttcattgg taaatcgtga gggtcaaagg ttttatcttt aaacatccga tatagtgtac
    11341 gtaccgaaca agcaatagga aactctgctc gaccaataag gacatctgga gtccaccctt
    11401 gagccacctt tttttgaatg tattcttgtt gcccatctgg taacacaatc ggacgtcttc
    11461 cgcagttacg tttgtttttc ttatattgtt catagtagct tattgcgctt ccaccagatt
    11521 taagaaaacg atagacatta taaatggttt gtcgagaacg ttgtaccaga tgggctactt
    11581 ttgaaacaga gtgtgattga ttgaaataag cttctatcat tacgagttca gttggtgtaa
    11641 gatgggtata ggtcatttgt actcactcct tataattctt tcgacggaac tattttgagt
    11701 gtagcacaaa tgactttttt agttgtctag cttaatttta caatcggcga tttctaattt
    11761 tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
    11821 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
    11881 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
    11941 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
    12001 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
    12061 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
    12121 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
    12181 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
    12241 ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac
    12301 acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa
    12361 gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca
    12421 gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct
    12481 gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt
    12541 ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa
    12601 gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg
    12661 tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt
    12721 tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc gattgctgct
    12781 tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta
    12841 ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta
    12901 tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct
    12961 gtttccgagg caccgttttc agcaccagaa gagcttatgg ttgttgaagg ttctgtttga
    13021 gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca
    13081 attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt
    13141 taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt
    13201 tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc
    13261 ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt
    13321 tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc
    13381 gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc
    13441 agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata
    13501 cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga
    13561 caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga
    13621 gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc
    13681 tgtgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag
    13741 caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg
    13801 ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg
    13861 ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca
    13921 cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat
    13981 ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat
    14041 acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc
    14101 cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc
    14161 gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt
    14221 ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca
    14281 aatactcgat tgtatcttcg atcacaattt cttcattatg ggctggcacc atgatcgtga
    14341 tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa
    14401 acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga
    14461 gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg
    14521 agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag
    14581 atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa
    14641 acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata
//
LOCUS       DASHWH010000066        14375 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.069, whole genome shotgun sequence.
ACCESSION   DASHWH010000066 DASHWH010000000
VERSION     DASHWH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.069"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            142..523
                     /locus_tag="U2R41_002157"
                     /pseudo
     CDS             142..523
                     /locus_tag="U2R41_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="U2R41_002158"
     CDS             654..1604
                     /locus_tag="U2R41_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8889547.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="U2R41_002159"
     CDS             2122..2577
                     /locus_tag="U2R41_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8889548.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2918..3520
                     /locus_tag="U2R41_002160"
     CDS             2918..3520
                     /locus_tag="U2R41_002160"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889549.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3614..3946
                     /locus_tag="U2R41_002161"
     CDS             3614..3946
                     /locus_tag="U2R41_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEM8889550.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="U2R41_002162"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="U2R41_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="U2R41_002163"
     CDS             complement(5081..5848)
                     /locus_tag="U2R41_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8889551.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="U2R41_002164"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="U2R41_002164"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEM8889552.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="U2R41_002165"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="U2R41_002165"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEM8889553.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="U2R41_002166"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="U2R41_002166"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEM8889554.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="U2R41_002167"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="U2R41_002167"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM8889555.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="U2R41_002168"
     CDS             9249..9566
                     /locus_tag="U2R41_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8889556.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="U2R41_002169"
     CDS             9566..11272
                     /locus_tag="U2R41_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8889557.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="U2R41_002170"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="U2R41_002170"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM8889558.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="U2R41_002171"
     CDS             12843..13115
                     /locus_tag="U2R41_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEM8889559.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="U2R41_002172"
     CDS             13136..14035
                     /locus_tag="U2R41_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM8889560.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4695 a         2371 c         2771 g         4538 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttccttttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcg
//
LOCUS       DASHWH010000067        14116 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.070, whole genome shotgun sequence.
ACCESSION   DASHWH010000067 DASHWH010000000
VERSION     DASHWH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.070"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..554)
                     /locus_tag="U2R41_002173"
     CDS             complement(<1..554)
                     /locus_tag="U2R41_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM8889561.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(570..1049)
                     /locus_tag="U2R41_002174"
     CDS             complement(570..1049)
                     /locus_tag="U2R41_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEM8889562.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1055..2079)
                     /locus_tag="U2R41_002175"
                     /pseudo
     CDS             complement(1055..2079)
                     /locus_tag="U2R41_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2096..3202)
                     /locus_tag="U2R41_002176"
     CDS             complement(2096..3202)
                     /locus_tag="U2R41_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM8889563.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="U2R41_002177"
                     /gene_synonym="adhC"
     CDS             complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="U2R41_002177"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEM8889564.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="U2R41_002178"
     CDS             complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="U2R41_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEM8889565.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="U2R41_002179"
     CDS             complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="U2R41_002179"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEM8889566.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8173..8871
                     /locus_tag="U2R41_002180"
     CDS             8173..8871
                     /locus_tag="U2R41_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM8889567.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9039..11678)
                     /locus_tag="U2R41_002181"
     CDS             complement(9039..11678)
                     /locus_tag="U2R41_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8889568.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12297..13328)
                     /gene="queA"
                     /locus_tag="U2R41_002182"
     CDS             complement(12297..13328)
                     /gene="queA"
                     /locus_tag="U2R41_002182"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEM8889569.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13521..>14116
                     /locus_tag="U2R41_002183"
     CDS             13521..>14116
                     /locus_tag="U2R41_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8889570.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3957 a         3096 c         2543 g         4520 t
ORIGIN      
        1 gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc agcaacaagt
       61 gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc tgacgcgata
      121 ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg gatcaacact
      181 ccaccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa taaagcattc
      241 cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa
      301 tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag aacgaaaaac
      361 ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc tgaactaaaa
      421 gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa tgcaatactt
      481 atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa cactaatccg
      541 gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc tggcttgttg
      601 cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc cgatacaggt
      661 actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg ccccattgtc
      721 aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac cagaaataaa
      781 ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc acttaaaacc
      841 gttacggcta aacgtgcttt ttgtcccaca acatacgcgg aagacaacat ggcaaaccag
      901 acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa aaccgttctg
      961 gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc tcccaggatc
     1021 tctaatacct tgtttaaaat cattttcatt acagctacgc tccttgctct tggatctttt
     1081 gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg gcaactgcct
     1141 ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct tgtgctaaag
     1201 ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg
     1261 ctgcttcttc aatggctttt tcctgttcat cagttagctg ttctttgatg gcatcattca
     1321 tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta gctaccccgc
     1381 catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc aaattggatt
     1441 gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct aaaagctcta
     1501 ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc aaatcttccg
     1561 gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca taataggtca
     1621 atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat
     1681 ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaacggaa aaaatcgaat
     1741 aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg
     1801 actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga
     1861 tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa
     1921 ttactggctg ttgcttgcat aagcaaagcg caaggtaact atctcattta cgtctgcatc
     1981 tgcgacagtt gccacactag aattatttcc gcagccgctt agacctagca gacctaggct
     2041 aagcgaactt gctgcaaaca gcagtgatgt ttttttcata tgggtttcct ttcttttaac
     2101 aataagagac gagcaattct gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg
     2161 ctgtgattcc gatattccga taaaaatcta aactgtcccc cattcctgtc ccgattgaaa
     2221 cgttttgaac ttctcctttt tcatcgattt gttcaagcaa tccttggata gcacgataag
     2281 cgacttcttt atattcttgt ggtaaataac gtttatggac cgctttcaaa ataccgtaag
     2341 caaatcctgc agttgcagaa gattctacgt aagaagaagg atcatccaac aacgtatgcc
     2401 ataaaccgct ttcagactga taagttttta atgcacgcac ttgtgcttca agtgtttcga
     2461 tcaataattt gcgcaatgca tcttctttcg ataattctaa gatttcaatg atctctggaa
     2521 tagcaatcgt caaccagcag tttccacgag cccaaaatgc ttcggcatag ttatgatttc
     2581 cttcaaacgt ccagccgtga taccacaatc ctgttcgttt atccatcaag tatttgatat
     2641 ggatcataaa ctggtaacga gcttcttcta aatattctgg gcgattcaac agtttcccga
     2701 tttttgccaa aggaagcact gtcatcatca atgtatcgtc ccacaactga ttcttatttt
     2761 ccggtccata tgtggcatgc tgtaacccgt cttcatttgt ccgaggcatg tcattcatga
     2821 cccattctgc ccattgttct aaataaggca aatagcgtga atctttcgtg tcttcataca
     2881 agtaagccat agtcaatagc ggtgccattg tattgacgtt tttgggcgga gcgccttctt
     2941 ccatacgatc ctcaaaccat tcattgacca cttgataagc gcgaggattt tttgtcatct
     3001 ggtaattttt ataaatacca tatagaccga caccttgtgg ccaattccac actgtccagc
     3061 ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga
     3121 tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt cactaattct ttaggtacaa
     3181 aaatctcttc tttaataatc atctttacgc caccttttta aacgctttca ttttatatcc
     3241 ttaatatagc agtaataccg tccactataa atatcgcttt ttgaacaaaa actgtctaaa
     3301 attgacctaa atgttaaata acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa
     3361 cttggagaaa caacgttttc caagttcttt ttcatctatt ttcttatctt ttttctacgc
     3421 tttcttttgc ttatccgttg taagcatcaa tgatgatctg tttcaattca gagatcaatg
     3481 gttctttcgg attagctgtc gtacattggt cttcgtaagc taattcagcc atacggtcta
     3541 cggttgtatc tagtgtttct tgtgtgactc cttgagcttt tagactcatg ttgatgccga
     3601 cagattttcc taattcataa acagccgttg ctaaagcttc gactaattcc tctgttgttt
     3661 cgcctttcag acctaagaat tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt
     3721 catatttagg gaacatcgca tgtttttgtg gatcttttgc attatagcgg ataatatgcg
     3781 gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta ttccccgccg attttatgtg
     3841 cgactgagtg acaaatacct aggaatgcgt tagcaaacgc cataccagcc attgtagaag
     3901 cgttgtgcat tttttcacgt gattccatat caggtgtttt tactgatttt tctaaatagt
     3961 caaagactaa cttgattgct tgtaggctca atccacgtgt ataatctgaa gccatgactg
     4021 aaacatagga ttcgattgca tgtgttaaca catccatccc ggtatctgct gtgacagatg
     4081 ctggaactga catgacgaat tgaggatcga cgattgcgac atctggcgtc aatgcataat
     4141 ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt
     4201 cagaaccagt ccctgatgtt gtcgggatac atacgaattg tgttttttct ggtttgtcga
     4261 tcttatatgt ccgtttacgg atatccaaga atttttgttt tgcaccaaag aaagatgtgt
     4321 ctgggtgttc atagaacatc cacattccct tagctgcgtc cattgcagaa ccgcctccaa
     4381 gagcgattac tgtatctggc ttgaagtctg ccatcagttt cgttcctgcg tatacggtat
     4441 ttgtcgatgg gttcggttca acatctgaga agacttcgat cttcacatca tttttacgtc
     4501 gtgccaatac ttcacgaact gtatcgcagt aaccgaattg gaccatacca gggtcacaaa
     4561 cgatcatgac acgttcaacg ttttccattt tctctaagta caatagagag ttcttttcaa
     4621 agaagatttt tggcggtaat ttgaaccatt gcatgttatt tctccgtttc gctacagttt
     4681 tgacattgat taagtttaca gcagagacgt tacgagaaac agagttctta ccataagaac
     4741 cgcatcccaa agtcaatgac ggaatcattt cgttatagat atcaccgatg ccgccttctg
     4801 cagatggtgt attcactaaa atacgacaag ctttcattcg aagaccgaat ttgacatgca
     4861 actcttcgtt ttctgaatgg ataactgctg tatgtcctaa tcctccaaga ttcaacatac
     4921 cttcacacaa atcaaacgca tggtccgtgt tgttagcttt gatcatggca agaactggag
     4981 aaagtttttc tcttgatagc ggataatctg ggcctacgcc ttccaattca gcaatcagca
     5041 ttttcgttcc ttttggtaca ctgattcctg ccaattttgc gatttccatc gcagaatgtc
     5101 caacgattga tggattgacc gcatattttc cttcgttcat cactgcatct tctaattttt
     5161 gcaattcatc tggttttacg atataaactt ggtgtgcttg gaattctgct tttactgctg
     5221 catagatttc tttatccacg atcacagctt gttcagaagc acaaatcatt ccattatcaa
     5281 atgttttcga aacgatcaaa tcgttcactg cacgtttaat ttttgctgtt ttttcaatat
     5341 aagctggtac gttacctgga cctacaccta aagctggttt tcctgtagag taagcagact
     5401 tgaccattcc agctccacct gtcgctaaga cgatcgcgat acctggatgg ttcattagca
     5461 tagaagtcgc ttcgatcgaa ggatgttcga tccattggat acaattttca ggagctccgg
     5521 cagcgatagc agcatcacgt accactcgtg ctgcttctgc agaagatttt tgggcacttg
     5581 gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg
     5641 ttgtggaagt cggattcgtt gttggagtta ctccgcaaac cacaccgact ggttcagcaa
     5701 tttcaatcaa ccctttttgg acatcttcat tgatcacacc aactgtttta tcatgtttga
     5761 tattatgcca aatatattcg gacgcataca tatttttgat tgctttatct tcgtaaacac
     5821 cgcggcctgt ttcctctaca gccatcttcg ctaatggcat atgttgatcc aaagctgcca
     5881 ttgccatttc atgcacgata tggtctactt tttcttgatc aaagctttcc atttctttca
     5941 atgctgcatt tgctttttct gccaaatcat caatcattgc ttgtacgtca attgtcttcg
     6001 ttttttcttt ttctattact ttagccatca ttatcctcct aatgggtcat ggttatttgt
     6061 taaataattc acatgtatat catacaatat ttatttttct atgtaaacac ttttttgtga
     6121 tttttttcac agttattttt aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa
     6181 aatatataaa gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa
     6241 tatatcccat gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct
     6301 gccctacctc gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct
     6361 tccagcgttt ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt
     6421 tcaacagtag tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct
     6481 gcacgatcgt actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg
     6541 tcgatttctg aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc
     6601 atgctatcta acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa
     6661 aaccacattg ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc
     6721 acctcactaa ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt
     6781 tttttactta atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac
     6841 ccgtattctt caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga
     6901 cgtacttgtt tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc
     6961 ccaaatgttt catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat
     7021 gtataacaat cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc
     7081 actactagac ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag
     7141 acacagtcaa acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg
     7201 acacccatca agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca
     7261 cgattcattt ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa
     7321 tccatcccga ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg
     7381 atgattccaa ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc
     7441 catcgagacg tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga
     7501 cattcgtcaa aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt
     7561 tctggtgata aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct
     7621 tcgatattcc gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc
     7681 ggttgatccc agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca
     7741 gggcgtagcc ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac
     7801 tcttctgggg acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg
     7861 gggaaagttc cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc
     7921 ttgattaaac gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa
     7981 tatcttgaca actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct
     8041 tccttctact ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag
     8101 aaaaacatta tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag
     8161 gagggaaaaa tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa
     8221 ggacgttggg gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact
     8281 attcttctta ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg
     8341 agcgtctcag aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt
     8401 tccagcatca ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta
     8461 cgtggacaaa aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg
     8521 tttatcggcg gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt
     8581 ttgctattag ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt
     8641 tactacgata tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca
     8701 agtcgaaaat tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc
     8761 ggctggcata tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac
     8821 atttctgcaa caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg
     8881 tgacttgtga ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac
     8941 attcagccca ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat
     9001 agttccagta aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc
     9061 ggtaatcgat ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct
     9121 accaagaacc agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc
     9181 agcaaagcaa cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta
     9241 ttggtcgcta aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga
     9301 atcgatgaat aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct
     9361 acccatgggc tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg
     9421 gtgaagaccc ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca
     9481 tttttcggaa tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca
     9541 aatcctggaa gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt
     9601 gctccccatc ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt
     9661 agcaaaaagt tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg
     9721 tctacgatcg tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca
     9781 tctgttcctg tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca
     9841 ttcacaccat ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc
     9901 cggatcttgt cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct
     9961 aattcttcgt ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc
    10021 aagataccta attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg
    10081 actgttttga tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt
    10141 ggatcgatca ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa
    10201 atatcttgcg gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt
    10261 ttaccaaagc gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga
    10321 gcaagaacat cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact
    10381 gttgtcatca tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct
    10441 tctaattctg cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt
    10501 ggattacctt tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt
    10561 gcagccattt tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact
    10621 tcatcttctt tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt
    10681 ttgtctgagc agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg
    10741 atcgcatgtt tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca
    10801 gtcaaggttt ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg
    10861 agtggttctc cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc
    10921 atactgattc gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta
    10981 ttttcgttca aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg
    11041 atcgctttcc cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca
    11101 cctaatgact catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct
    11161 ccagtcaacg ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca
    11221 gaaatagcat caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca
    11281 acagtggtca ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag
    11341 gattccagtg ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca
    11401 acgatagcga tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta
    11461 taaaaggaaa tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga
    11521 aagaattttt tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt
    11581 tttcgccgtt catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc
    11641 ttctttactt cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt
    11701 cgtacaaaaa aaggtactta ttttactata acatcctttt tttatttact tcaaataaag
    11761 ggcttatcga ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct
    11821 accaaaggat gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa
    11881 tttctcgata gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca
    11941 tgcgtgtaga gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta
    12001 tccgaaacaa gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga
    12061 ataggaacaa gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac
    12121 atgaagaaat tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt
    12181 atttgaatcc ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg
    12241 tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat
    12301 ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg
    12361 taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat
    12421 tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta
    12481 atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc
    12541 gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt
    12601 acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg
    12661 tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc
    12721 agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc
    12781 aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga
    12841 tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca
    12901 agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat
    12961 tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg
    13021 gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga
    13081 aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact
    13141 cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa
    13201 tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg
    13261 tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt
    13321 gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt
    13381 ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta
    13441 ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa
    13501 atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg
    13561 gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta
    13621 tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc
    13681 caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat
    13741 tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc
    13801 gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa
    13861 cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac
    13921 atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc
    13981 aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct
    14041 tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc
    14101 atgacatgga tgaagc
//
LOCUS       DASHWH010000068        14107 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.071, whole genome shotgun sequence.
ACCESSION   DASHWH010000068 DASHWH010000000
VERSION     DASHWH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.071"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            66..767
                     /locus_tag="U2R41_002184"
     CDS             66..767
                     /locus_tag="U2R41_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEM8889571.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="U2R41_002185"
     CDS             764..1885
                     /locus_tag="U2R41_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEM8889572.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="U2R41_002186"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="U2R41_002186"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEM8889573.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="U2R41_002187"
     CDS             3264..4172
                     /locus_tag="U2R41_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEM8889574.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="U2R41_002188"
     CDS             4207..4479
                     /locus_tag="U2R41_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEM8889575.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="U2R41_002189"
     CDS             4490..5536
                     /locus_tag="U2R41_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEM8889576.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="U2R41_002190"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="U2R41_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEM8889577.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="U2R41_002191"
     CDS             complement(8547..9233)
                     /locus_tag="U2R41_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889578.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="U2R41_002192"
     CDS             complement(9211..9705)
                     /locus_tag="U2R41_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM8889579.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="U2R41_002193"
     CDS             complement(9725..10945)
                     /locus_tag="U2R41_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889580.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="U2R41_002194"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="U2R41_002194"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEM8889581.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="U2R41_002195"
     CDS             complement(13021..>14107)
                     /locus_tag="U2R41_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8889582.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2592 c         2920 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DASHWH010000069        14088 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.072, whole genome shotgun sequence.
ACCESSION   DASHWH010000069 DASHWH010000000
VERSION     DASHWH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.072"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1406
                     /locus_tag="U2R41_002196"
     CDS             <1..1406
                     /locus_tag="U2R41_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8889583.1"
                     /translation="VEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTP
                     GNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTN
                     LPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADK
                     YNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPD
                     GSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWK
                     EKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKT
                     EPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVV
                     SSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLIL
                     LGTLGLLGYKNRKKENE"
     gene            1540..2883
                     /locus_tag="U2R41_002197"
     CDS             1540..2883
                     /locus_tag="U2R41_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8889584.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2995..4446
                     /locus_tag="U2R41_002198"
     CDS             2995..4446
                     /locus_tag="U2R41_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEM8889585.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4795..5427
                     /locus_tag="U2R41_002199"
     CDS             4795..5427
                     /locus_tag="U2R41_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEM8889586.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6015..7454
                     /locus_tag="U2R41_002200"
     CDS             6015..7454
                     /locus_tag="U2R41_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8889587.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7749..7967
                     /locus_tag="U2R41_002201"
                     /pseudo
     CDS             7749..7967
                     /locus_tag="U2R41_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8216..8431
                     /locus_tag="U2R41_002202"
     CDS             8216..8431
                     /locus_tag="U2R41_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889588.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8648..8904)
                     /locus_tag="U2R41_002203"
                     /pseudo
     CDS             complement(8648..8904)
                     /locus_tag="U2R41_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9091..10002)
                     /locus_tag="U2R41_002204"
     CDS             complement(9091..10002)
                     /locus_tag="U2R41_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8889589.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10021..11025)
                     /locus_tag="U2R41_002205"
     CDS             complement(10021..11025)
                     /locus_tag="U2R41_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8889590.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11022..13145)
                     /locus_tag="U2R41_002206"
     CDS             complement(11022..13145)
                     /locus_tag="U2R41_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8889591.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13150..14070)
                     /locus_tag="U2R41_002207"
     CDS             complement(13150..14070)
                     /locus_tag="U2R41_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8889592.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4601 a         2504 c         2651 g         4332 t
ORIGIN      
        1 cagtagaagg cgaaaaagta gaagttgggg gcacagtaga tctaacagat aatgttacta
       61 acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc ggtacaattg
      121 ataccaacac accaggtaat tacgaaggtg tcattgaagt aacgtatcca gacggtacga
      181 aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctgacgcc gataaatatg
      241 aaccaacagt agaaggcgaa aaagtagaaa ttggaggcac agtagattta acagataatg
      301 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      361 caattgatac caacacacca ggcaattatg aaggtgtcat tgaagtaacg tatccagacg
      421 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct aatgcagata
      481 aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca gatgcatctg
      541 agggaattaa gaacaaagaa gatttaccaa aaggaactac ttatgcttgg aaagagaaag
      601 tagatgttag cacagctgga aataaaaaag gtactattgt agtgacatat ccagatggat
      661 catctgatga agttgaagta gacgttacag tgacagatac tcgttccgat gcagataaat
      721 ataatcctga aggacaaaaa gtgactacag acttaaataa agagccagat gcatctgagg
      781 gaattaagaa caaagaagat ctaccaaaag gaactactta tacttggaaa gaaaaagtag
      841 atgttagtac agctggaaac aaaaaaggta ctgtagtagt aacatatcca gatggatcta
      901 aagaagaagt agaagttact atttctgtag tagataaaaa agcaccgaat aaacctcaag
      961 ttgatcctat tacagagggt gaccaaattg ttactggtaa aactgaacca aatgcagagg
     1021 tgacagttac attaccagac ggaagtcaac accatgggac agctgataaa aatggtaatt
     1081 ttacagttaa agttcctaaa ttagaggcag ggacaaaagt gatagtaact gcaactgatg
     1141 aatctgggaa tactagtgaa cctactaatg tagttgtttc ttccaacaaa aaagacagtg
     1201 gaaaaaacgg tagtaaaggt agtaagactg acaatcaagg tagcaacagc aatcaagaca
     1261 aaaatagagg caaaagccag tcctcaaaag tttttcctaa gacgggagag agtgacagta
     1321 atatctttac tattagcggt ggtttaatac tgttaggaac gttaggatta ttagggtata
     1381 aaaatcgaaa aaaagagaat gaatagaaaa tcaatttctt aagtcttctc tgagaagagg
     1441 aagaatcttt ttcttcctct ttttgctttt ttatgatggg aaataagtgt tagttaaaga
     1501 tgaatatact ttttatagaa aggaaaagaa tgtaattaag tggaaaaaat agtaattatt
     1561 ggttcaaacc attcagggct agccgcagct cgatttctaa acttaagcga ttcaaattat
     1621 gaagttattc taatcgataa aaaatcagaa attagttact tggaatgtgg gacatcgtta
     1681 tttataaaag gagaagtaag taatttagaa gatctttttt atcaaaatag aatgacacta
     1741 aattctgaga taaaaaagtt atttctagag acagaagttg acaaaattga tattcaaaat
     1801 aaaaagatta gttgtgtaaa aaaagatggt acaagattct ctcaagaata tgacaaagtt
     1861 atattagcta caggttcaaa gcaaattatg ctagatatac cggggaataa tttagctaat
     1921 attttttcga taaagaattt tccaaatgca aaagagattt ttaaagcttt ggatgatagt
     1981 actattaaaa aagtaggaat tattggagca ggatatattg gagttgaaat tgctgaagcc
     2041 ataagaaaaa gagggaaaga agtatatctt ttcgatgttg cagatagagt gttgtcgact
     2101 tattacgacc gtagtttttc agataaagtt gaagaaatac tatcaaaaaa tggaatacat
     2161 ctttgtctat cagaaagtcc taaaaaatat ttaggaagaa aaaagattga aaaaatagtc
     2221 actacaagtg gtacccaata ctctatggat atggtcgtac aagctatagg atttctatct
     2281 aatagtccaa ttggggaaaa ggaactcttg agagatgtta gtggagcata tctaactaat
     2341 gaataccagc aaacaaatat aaaagatata tatgctattg gggattgtgc tacaactttt
     2401 tttacgtcca ttaatagaag tagtgttgat ttttctattt ctaatgctct aagaacagct
     2461 tatattgcaa ctcaacatat taacaatcaa gattttacac cttctggttc ccaatttaca
     2521 aatggctttt ctatatttaa ttacaatttt tacgcagcag gtttgaattt aaaaacagca
     2581 cgattattaa gtgagtctat agattatata gaaatagaag atttcattag acctgctttt
     2641 ttgagaaaaa atagtcgtgt aaaattaaga attagctttg aaaaaaatac aaagagaata
     2701 gtaggtgtac aactttgtag tcaagaagat ttatctggac tattatcaat gttttctctt
     2761 gctatagaag aacaaattac attagacaaa ttgaaaatgt tagattattt atttcatcct
     2821 agttttagcc aaccctataa ttttatttta aaggcagtaa caggattaaa cattgaaaca
     2881 taatcgttac taaatgaaat caagaaatat actgtattac tcaattcatt gaaaatattt
     2941 atcataattt ttaatagaaa acactattaa agagaaaaaa gagaggtaaa tcaaatgaaa
     3001 aaatctttaa aacttgcatc agcagttata ttaatgtccc aactccttat agcaacacct
     3061 ttccatgtgt tggcaagcga aaattcaaca tacaaaattg caaaagaaag aaccgagacg
     3121 actgaaatga gtaattccga tgtaacagca gagagtagtg aacttttgac aacttcatca
     3181 agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt
     3241 acgacttcaa atgccgagtc ttcaacaact tcatcaagtg aaagtagtac gacttcaaat
     3301 gccgagtctt caacaacttc gtcaagtgaa agtagtacga tttcaagtac cgaatcttca
     3361 acaacttcaa gtagtacaag tgaaataact tcttcgaaag tgacaaaacc aaaccatata
     3421 gaatcttcaa aaaaggaagc gccaaaaacg gaaggacaaa caaaaacttt ttcgacacca
     3481 cagcaaatag aatcaacaga atctttccaa tcacaaccaa tactatctgg taatgaagaa
     3541 attcattttg acaaaaatga atctgtagaa aattttatta tgaaaattgg tgagtctgcg
     3601 agaaaaattg gtcagaaata tgatttatat gcttctgtta tgattgcaca agcgatctta
     3661 gaatctgcca gtggtcaaag ccagttagct caggcaccga attacaattt atttgggata
     3721 aaaggaactt ataatggaaa ctttgtcatt atggtaacaa acgaagattt gggtaatgga
     3781 acgctttata cgacccaatc aaaatttaga gtgtatgaaa actatgagga aagctttgaa
     3841 gattatgcca aattattaac taagggaatt agtggaaata aagattttta tgcaggagca
     3901 ttaaaagcta atagtaaaac atatcgagaa gcaactaaat tccttacagg acggtatgca
     3961 acagatacac aatactattt gaagctaaat gaattaatta aaacctacga tttaaccaac
     4021 tatgataaag aagttaaagg tccacaaatg agtaagactg gataccaagt gcctattcat
     4081 aattattcaa taaccagttt gtttggaaat cgtggaaatg aatttcaccg aggacttgat
     4141 atggcagcta cacagggtga gccgattcat gcagttaaag ctggtaaggt tgttaaagca
     4201 gagtttcact actcttgggg aaattacgtg gtgatacaac acgaagatgg atcaaccgcg
     4261 ttatatgctc accagcaaca atacctagta aaaactggag ataatgtttt gcaaggacaa
     4321 ataattggct atgtaggaag tactgggaat agtactggcc cacaccttca tttggaaatt
     4381 tgcaaagata gtagtctttc tcagagcatg ttagttgatc cgaaaacctt attattcgga
     4441 gactaaaaaa ggtaatcaat ctgacaaagt aacaagaaaa tggttggatt cttagacctt
     4501 tgatcaggat attgagactg gaattgatat ctcgattacg atgggtttga caaataaaac
     4561 aagtccatta tcgaatatcg agtgtattat tgccgtcttt atgctcatat tttgagcagg
     4621 tttcactaga tgaaaacaat ttatctatct tgaggatttc gtgtacgtat cgttcagtct
     4681 tgtattgtaa attagtcata aaatcagact aagaaaaatt ggaaatattt ttttctgatt
     4741 tattaaaagt taattgaaat tatatggtac ttctttcttg ggagggtagg tgcagtgaag
     4801 gaaagatatg gtcaccttac tgttgttgag gagattactc ataatggata taaaaaatat
     4861 cgttgcctat gtgactgtgg gaatgaaacg attgtatatc gatcaaactt actatcaggt
     4921 agaaccaaaa gttgtggatg tggaaattat aaaaataggc ataaatataa aaatttgaca
     4981 aataaaactt ttggtaagtt agttgcaatc aagccaacta aactgcgaag tgaaggaact
     5041 gttgtttggg aatgtcaatg tctgtgtggg agaaaaaagt tagtatcggc aaagcgactt
     5101 ctaaaaggtg atgtaaaaag ctgtggatgt ttgaaatcaa aaaagcatca cattgaaaat
     5161 aaaaaattcg gattacttac tgcaaagtat cctttggaca ataactgttc atggaaaaca
     5221 aaatgggttt gtcattgtga gtgtggcagt atgtgtgaga taagttatag taacttattt
     5281 tttggacata ctcaaagttg tggatgttta aaaaggcaag aaacaaggac atgtgttgaa
     5341 gggacagtaa tagagtgtct aacgtcaaaa ttaccgaaaa ataatacaag tggtgttaag
     5401 ggagtttatc gttctaaaga gaaatagatt gcgtatataa ctttaaaaaa aaagaggtac
     5461 tatttaggat cgtatgattc tattaagagt gcagctcaag ctagaaaaga tgctgaaaat
     5521 aaattgtttc aacctttgat tgacaaatat cgaggtaatc attaattatt gtggataata
     5581 attaacagtg atatataaac atctataata tattattcat actattgata tttagtataa
     5641 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
     5701 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc ttacgtctca
     5761 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
     5821 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctccaa
     5881 cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat ggctagagtc
     5941 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
     6001 aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
     6061 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
     6121 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
     6181 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
     6241 atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggatcgc
     6301 tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg gtacaagcca
     6361 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
     6421 atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc tcgattgctg
     6481 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
     6541 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
     6601 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     6661 gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     6721 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     6781 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     6841 cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct ttttatctgc
     6901 aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc tttcctttga
     6961 tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc ccggagattt
     7021 tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg tttttgtcag
     7081 tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     7141 tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa aacttgggac
     7201 tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg atttcgcttt
     7261 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta actaactata
     7321 tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     7381 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     7441 agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt atgggtgagt
     7501 gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa actcaagtat
     7561 tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa gactgcgaaa
     7621 gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga ggctactggt
     7681 tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga ttatgtaaag
     7741 attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg tctagtcaaa
     7801 ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga gaatggactt
     7861 gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat attttgacca
     7921 agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa caacggggat
     7981 agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt tatcaagaat
     8041 tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc atttttgatt
     8101 taggttcctt ttctaattat aataagatga ccactacagt aacctactga aatatataaa
     8161 cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca ttcttatgaa
     8221 cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta cttccaattc
     8281 aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag
     8341 atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg ctatgaaaaa
     8401 tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac ttgccactta
     8461 gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag agcatacttg
     8521 caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc ccgtagttct
     8581 aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg acccttagaa
     8641 agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt cggttttagg
     8701 ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat ctaaggtaat
     8761 gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca ttacatcatc
     8821 aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa gaaaaactga
     8881 atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga ctgattatga
     8941 catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt accgaatggc
     9001 attcatcacc cacctataga ggatgggcga cttctgcctg aaataagtta cactacttat
     9061 aacttctaat aaaattagat tgagtcatta ttatttaaca tcaacgattt tccagtttcc
     9121 atttttttgt aatactaatt tgtactgaaa gtagctagtg gtttttgata cattatcaag
     9181 gtattttatc tttgtactca ctagaaaatt ttttccgttt tgtagataaa ctggattctc
     9241 taactccgaa aactttaagt caatattgat tggtcgcatc gcaccatttt caacataata
     9301 ctccaattct ttttcagaag ctgatggata aagtttaaaa aatgtttcta aaaaatcggt
     9361 gatttcttcc gttgttttgg catcaatttc tgaatcgttt tctacttgtt ttgcttcata
     9421 ttttgccttg tctggctttc caatcattgt tggcaaacta gttactaaat gattcctgtt
     9481 ttcatcttca aagattgtca ctcgatatgc agaagaaatt gattgcgtct tttttccctc
     9541 tgtaatatgt tgctccacta aaaatgaaac gacaaattct tccgagtgtt tttcaacatc
     9601 tagaattttt acagattgaa cttcggaact cgtgggaata tctgctcgaa ccatatcttg
     9661 gattaaagcg agaccgtttt ctgtaaggta ctgttcgaga ttactcattc tcttttcaat
     9721 tgcttctttg ttatttttcc atgaaaaata ttctttcgca aaatcctctg taaaactttc
     9781 aacgccagaa gtattcacta ccttctcttt tataaccact ttttcatgag ttgtatgttg
     9841 atcaatggcg gtgaaatttt tataaacgcc aaaagcaaga ctacaagaca ataaaagcca
     9901 aagggcaagc accatttttc gatgcttacc cacggataca actcgttctt ttttcttttt
     9961 tgggctcttt tccttttgat tccgttcaat ttttatcttc attctttttc ctcttttctc
    10021 ttatttgtat cggccagctc cgataagatg ctgccgccaa tagttttccg ttagattcgt
    10081 ccaccccact ggatctcctg catgaaacat gcgattctcc cctgcgtaaa tgcccacatg
    10141 ggtcacataa gtaccggcat cataggttga atgaaagaaa actaaatcac ctggtttggc
    10201 atcttttaga tcaatatgag tcatggcatc atattgatcc tgagccaccc gaggaagttt
    10261 caggtcaact ttggcataac accattgggt caagccacta caatcaaacc ccgtttctgg
    10321 acttgagcca ccaaacacat aggccgttcc ttcatactta aatgcctcat caaaaatttc
    10381 ttggactatt ttgtcatcaa actgagttgt agttaggtat tgcttcacta ataaacagta
    10441 aaacatattg ccataattat accgccaccc accattttcc ttaatcgcga ttgggttggg
    10501 gtaatcgact ttttgaccac ctgatttttc ctttgaaaaa gattcagcta aagcaaaact
    10561 atgcttcttc ccgtttttcc cgacatgatc tagatacccg ccaccatagt tataggcctg
    10621 aataatacta tcttgatcac accctagttc tttggaacgc ttaaccaaag acgcaaaata
    10681 gagacagcct tgtttgatgg atgcctcggt acttaatgaa ttaggtggta atccggctga
    10741 ctcagaactt tgcataacgt cttccattgt gccaccagat tccacttgaa taatcgccaa
    10801 aagaattgga atatactctg ttatatccga ttctttggca tacttttcca ccattgcacg
    10861 gtgtttcaat acttcttctg agacactagt tccgccagga gaagtgccag aactaccatt
    10921 tccctcactg tcttcaccaa acaacaaacc cacacacatc aaaataccca taaaagagat
    10981 ggtcagaaaa gttaatgaaa taacacccac tttcttcaag ttcatttttt ctcacctgac
    11041 cttgattttg gacgatggtt cattcttttt tgtacttttt gattggtctg ctttggcaga
    11101 gtggacctct gtttcacttg ttggaaagaa tttttcttac ttgctgattg gcttttccct
    11161 tttgaaatga tttcttgact agttagtttt cgtttgattt cgggattcag atttgctctc
    11221 acaatccgct ttggtgcttc ttttttcagc atcatatctt tctgaggttg agctgcttta
    11281 tctattggaa tattcgccct tctttttccc tgacgatttg ttgcttcact cagtatcttg
    11341 cgtttttcgt ccattttcgc atttcttcgt tgtcgttgtt tttctcgcaa atccactcga
    11401 tccgcttttt cttggaacag tccgcgcttg aattcttctg gtgctttttt cgacttttcg
    11461 attcctttat ggagattgta tttcaaattc gttggcgtat tccttacctg atcctttact
    11521 tgtttagtct tatctttcac caaagcttgg gtatccaaaa ctttgccgat ttttctccca
    11581 gccaaattca ttcgggattg tttcttatta tttgaaactg gattctcctt agttaagtca
    11641 gatagtattt cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga accggatggc
    11701 ttaaatttgg attttgattg ttcctttgcc aacttatttc cgactgctgc accaccacca
    11761 aaagccaaag ctcgtcccac atttcgattg agttgccgca attttcgatt cattaacatt
    11821 tgcggttttc gcatgacgcg tcttcccatt gactggctat cattactctg gaggttgaac
    11881 atactcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat ttgcagaaaa
    11941 gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga gatgctaaat
    12001 gcagtcgtaa tcaccaaagt aacacccgct cgcatcataa tcgtattgaa taaacgaata
    12061 atcgccttct ttcctaaact ttcataagtc ggcaacatgg acaataagaa actaattggc
    12121 agaaacatcg caaagataat gaagagaatt tgcgagaaca acataatccc tgttagaaga
    12181 aaaacaaaga aggaaatgcc aatattgaac aataccaaaa agaccacggt ccctaaacgg
    12241 ttcatggtct tggtaatact gagattcgca ttgtcattgt cttcaatctc tgccttgacc
    12301 gcttcttccc gatctttgcc tttgttttca tctggactca cagatagaat cttgttgacc
    12361 cggtcttcac caatttcttc tatgttggaa tcatcaaatt gcagtagtaa ccaaggttgt
    12421 tggacttgaa tcgaaaacaa actatcccga atcaaatcca cactatcttt tccttggctt
    12481 tctgaattgg gtacaacgat ctcggttcca agggtaaggg ccgcttcact aacatctgag
    12541 ctaaagtcat tgatttttgt gatataggta ggcgcataag caatgaacga acccgatagt
    12601 agaaaaatca tcacaaaatt aatcacggct cgaactgctt tcgtggtttc tcgttttaat
    12661 aatccggtat acgccacata cacccccaga gctaaaatca tcaataatag aaacccaaaa
    12721 taaaatccgg aggtctggag tccattttca gtaataccag ccaatgtttg gatattcttt
    12781 ccaatacttt cagccgtatc cgaaatgaaa tctaatttgt aagcctcttg aaccacataa
    12841 cccgtggcgt tcgacaaata caaactcacc gtccaaatga aattagtaat ggcataaaga
    12901 ccatacatca cgcttttccc taagccatca ccccaattcc aaggcaacca atcccaagag
    12961 ctatccacaa agaaatctag ttgatagttg ttcagtgaat atttggaata tagatttcct
    13021 gcttcaactg tatcatcaac tagccctgtc gcctcggcta ccgtaccaac taaactcaat
    13081 agaactatca gagtgagtat gctaataaaa acgattccca caatccgaaa aagtttcttt
    13141 ttcatcccct cactccattc tctcttgaac aggtggacgg gtatcaaagg catggaagag
    13201 ttcttcaaag actggatgta cctgaaccac gcctacccga ccatacaaat cctgcatcaa
    13261 gcattggcca ttttccaaat ctcgtaggcg tttttgattg ctttcatctt ctcgatccaa
    13321 gccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa acgcaaactt
    13381 caacccgata ttattcttca agcgttcatc cagtaaatca tcactatttt gcgtgacaaa
    13441 gtaaaccccg gcattcatag cccgcccagc acgaacgagc ttattcgata atgttttccc
    13501 ttgcgctact tgaagaaaac tccacgcctc atctaagtct acaattttga agatttctcg
    13561 attactatgg ataaagtcca aggcaaaagt ggaaatgacg attagcatgg aaacactaag
    13621 caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa ccaaatccgc
    13681 tacttgaatg atatttaact gcctatctaa actaatcgac tgggtaatcg aaccatctga
    13741 gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg cgatattttc
    13801 cgcaactggt gagccttctt ttcgaagctc attaattact tgtaataatc cccgttgatt
    13861 gctttgggta accgtccgaa tggccttacg caacactgga aacttctccc catctcgact
    13921 agaaattcct gttaaaaagg tcagaatatc aattgctaga ctttcagaat ccttcttttt
    13981 ctccataatc acataaggat caagtaatcc ttgatttttt ggcttactag ttaagttgag
    14041 aatattgatt tcatccgcaa tttctggcaa ggtttctttc cagccacc
//
LOCUS       DASHWH010000070        13522 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.073, whole genome shotgun sequence.
ACCESSION   DASHWH010000070 DASHWH010000000
VERSION     DASHWH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.073"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(390..1337)
                     /locus_tag="U2R41_002208"
     CDS             complement(390..1337)
                     /locus_tag="U2R41_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEM8889593.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="U2R41_002209"
     CDS             1525..2712
                     /locus_tag="U2R41_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEM8889594.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="U2R41_002210"
     CDS             2693..3997
                     /locus_tag="U2R41_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEM8889595.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLFSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="U2R41_002211"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="U2R41_002211"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEM8889596.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="U2R41_002212"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="U2R41_002212"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEM8889597.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="U2R41_002213"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="U2R41_002213"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEM8889598.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="U2R41_002214"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="U2R41_002214"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEM8889599.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="U2R41_002215"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="U2R41_002215"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEM8889600.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="U2R41_002216"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="U2R41_002216"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEM8889601.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="U2R41_002217"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="U2R41_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..11657)
                     /locus_tag="U2R41_002218"
     CDS             complement(10485..11657)
                     /locus_tag="U2R41_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8889602.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            12228..13040
                     /locus_tag="U2R41_002219"
     CDS             12228..13040
                     /locus_tag="U2R41_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889603.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT         4636 a         2103 c         2584 g         4199 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacaaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tattttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc taagttaata
    10801 tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga
    10861 atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact tgcgttggtg
    10921 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
    10981 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
    11041 ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct
    11101 atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg
    11161 gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc gttaaccatt
    11221 ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt
    11281 tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt
    11341 gaagcaatga gggctttttc gtttcgttga taacgttcaa acactgtggg tgaaaaatgg
    11401 ccatcacgtg ttctgggtac ttttaattct agcgtgccta tacgtgtcgt aaagctgcgc
    11461 tcataatagc catttcgttg actttgtcgg ttttctgttc gttcatattc ttttgcttga
    11521 atatattctg ttcgttgatt ttccattagt tgattaaata ccgttgttaa aatattttta
    11581 gaaacgtcat cctttacaga atattcaata atgctttgaa tctcttcgct tttcagtgta
    11641 aaatgtactt gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta aaaacattgt
    11701 accgtaaaag gactgttaca tggccttttt acttttacac aattatatgg acgtaatcta
    11761 cttgttctat tgctagttat tgctagtctt tattcttttt tgttttttag ggatgtacaa
    11821 aagatctcaa ccacaggaga cattgctttt cacttttcta gaataaaatg cttgtcctca
    11881 gtattttctg gtccaataaa ttttaccaca tttaatagct atggcggagg cgtgaatttt
    11941 tttatccata tctaacatct ttccagccgt aatattttac tggacaacaa acaatttgat
    12001 tctaagttat atacttacta tacgttggga tactgaatat ctgtacaatt ctagtgagct
    12061 atcaatatgg acaaaagaat agatgcagcc ttcttattca gctgtttgta tagcttttat
    12121 ggattgatat atattagcgt actgcaattt cagaatcaat tgcgttgacg attgtaccag
    12181 ttgtcttgta ttacacatat actcttcttt atgaaagaaa aaactgcgtg attcttttag
    12241 ctgtcagtat gagtttgctc atctataccc atattctctc cacctttatg agtgtgattg
    12301 ttattggctt cttgtttatc ggctctcttt tctctaagcg gaaagagaga aaagaaatgt
    12361 cgaatctgat atcaaatatg ataaaagctg ttggttggac cattctttta acagcttacg
    12421 tttggtatcc gatgttacaa caagtcctac atcaaaaaat taatcgtcct tttcgcacaa
    12481 aacttcaaga aagggcatta aacatttctg atagtttaat cggtgcagta aataatgatt
    12541 tagttacatt tacaatggga acaattggtg tggtttcatt tattcttcca cttttcttta
    12601 aaaaaaaatt tgccaataaa gaaaagatta ttaatttttg tgcagtgacc acttggttct
    12661 tatcaacgaa tctttttcca tgggaatttt tacaaaaaac acctattcaa atcattcagt
    12721 ttccttggag aatcttgggt tttcaagtgc tttttggttc attgatctta attatagtgt
    12781 ttttaaagtg gaaaacaagc aacaaaaaaa gtatgtgtag ccttgtagga atagtccttc
    12841 ttatttttac agtgactgta gcaacagaag cgaattattc gcagaaaatt caatcgtata
    12901 aaggtcgttt aattatgacc aaaaaagatg ttacacttta tacaacctcg agaactggag
    12961 gtctttatga ctatgcacca ttagatgcat tgaaatataa agatcattta aagaaacatg
    13021 aagtaaaagt ctatgattga tgggagaatg catctcataa agcacataat tctaaaataa
    13081 tctatacagt aggagatgcg aatgagcaaa atatcacgtt acctgtattt aattactggg
    13141 aacagttatg aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc
    13201 tattaaaggt acgggaaaaa gaactatagt tgaggtttca tctacgtatc caactacgat
    13261 gattctatct ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat
    13321 aagatgctgc ggttaaatga aaatagatta gatagatatt ataaatatgt tgattctatt
    13381 ttcatagcaa tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacggt
    13441 gaatttattc cgaaaagtga aaagaacaaa tataaaattt aataaaaata tagaactaaa
    13501 taggaaaacg tagacgtaat tt
//
LOCUS       DASHWH010000071        13496 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.074, whole genome shotgun sequence.
ACCESSION   DASHWH010000071 DASHWH010000000
VERSION     DASHWH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.074"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..1364)
                     /locus_tag="U2R41_002220"
     CDS             complement(45..1364)
                     /locus_tag="U2R41_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8889604.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="U2R41_002221"
     CDS             complement(1361..2014)
                     /locus_tag="U2R41_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8889605.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="U2R41_002222"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="U2R41_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="U2R41_002223"
     CDS             complement(2724..3440)
                     /locus_tag="U2R41_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEM8889606.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="U2R41_002224"
     CDS             complement(3564..3749)
                     /locus_tag="U2R41_002224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889607.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="U2R41_002225"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="U2R41_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="U2R41_002226"
     CDS             complement(5356..6387)
                     /locus_tag="U2R41_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889608.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="U2R41_002227"
     CDS             complement(6394..7224)
                     /locus_tag="U2R41_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8889609.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="U2R41_002228"
     CDS             complement(7221..8036)
                     /locus_tag="U2R41_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8889610.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="U2R41_002229"
     CDS             complement(8033..8857)
                     /locus_tag="U2R41_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEM8889611.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="U2R41_002230"
     CDS             complement(8847..9947)
                     /locus_tag="U2R41_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8889612.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="U2R41_002231"
     CDS             10125..10910
                     /locus_tag="U2R41_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEM8889613.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="U2R41_002232"
     CDS             10943..11326
                     /locus_tag="U2R41_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM8889614.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="U2R41_002233"
     CDS             11402..11533
                     /locus_tag="U2R41_002233"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8889615.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="U2R41_002234"
     CDS             11502..11738
                     /locus_tag="U2R41_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8889616.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="U2R41_002235"
     CDS             complement(11816..12787)
                     /locus_tag="U2R41_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEM8889617.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4108 a         2931 c         2336 g         4121 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatg
//
LOCUS       DASHWH010000072        13145 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.075, whole genome shotgun sequence.
ACCESSION   DASHWH010000072 DASHWH010000000
VERSION     DASHWH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.075"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..149)
                     /locus_tag="U2R41_002236"
     tRNA            complement(77..149)
                     /locus_tag="U2R41_002236"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="U2R41_002237"
     CDS             530..1099
                     /locus_tag="U2R41_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEM8889618.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="U2R41_002238"
     CDS             1086..1946
                     /locus_tag="U2R41_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8889619.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQFLEG"
     gene            2136..2840
                     /locus_tag="U2R41_002239"
     CDS             2136..2840
                     /locus_tag="U2R41_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8889620.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="U2R41_002240"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="U2R41_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEM8889621.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="U2R41_002241"
     CDS             4677..5993
                     /locus_tag="U2R41_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889622.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="U2R41_002242"
     CDS             5994..6863
                     /locus_tag="U2R41_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEM8889623.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="U2R41_002243"
     CDS             6918..7727
                     /locus_tag="U2R41_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8889624.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="U2R41_002244"
     CDS             complement(7830..8459)
                     /locus_tag="U2R41_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEM8889625.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="U2R41_002245"
     CDS             8629..9921
                     /locus_tag="U2R41_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889626.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="U2R41_002246"
     CDS             complement(9997..10659)
                     /locus_tag="U2R41_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8889627.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="U2R41_002247"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="U2R41_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEM8889628.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="U2R41_002248"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="U2R41_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEM8889629.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2832 g         3680 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca atttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DASHWH010000073        12446 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.076, whole genome shotgun sequence.
ACCESSION   DASHWH010000073 DASHWH010000000
VERSION     DASHWH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.076"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            359..922
                     /gene="efp"
                     /locus_tag="U2R41_002249"
     CDS             359..922
                     /gene="efp"
                     /locus_tag="U2R41_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEM8889630.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            1023..1193
                     /locus_tag="U2R41_002250"
     CDS             1023..1193
                     /locus_tag="U2R41_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889631.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            1408..2667
                     /gene="lysA"
                     /locus_tag="U2R41_002251"
     CDS             1408..2667
                     /gene="lysA"
                     /locus_tag="U2R41_002251"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEM8889632.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            2654..3454
                     /gene="dapF"
                     /locus_tag="U2R41_002252"
     CDS             2654..3454
                     /gene="dapF"
                     /locus_tag="U2R41_002252"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEM8889633.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            3457..4338
                     /locus_tag="U2R41_002253"
     CDS             3457..4338
                     /locus_tag="U2R41_002253"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEM8889634.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            4335..5051
                     /locus_tag="U2R41_002254"
     CDS             4335..5051
                     /locus_tag="U2R41_002254"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEM8889635.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            5057..5752
                     /gene="dapD"
                     /locus_tag="U2R41_002255"
     CDS             5057..5752
                     /gene="dapD"
                     /locus_tag="U2R41_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEM8889636.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            5768..6769
                     /locus_tag="U2R41_002256"
     CDS             5768..6769
                     /locus_tag="U2R41_002256"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEM8889637.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            6766..8127
                     /locus_tag="U2R41_002257"
     CDS             6766..8127
                     /locus_tag="U2R41_002257"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEM8889638.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            8087..9238
                     /locus_tag="U2R41_002258"
     CDS             8087..9238
                     /locus_tag="U2R41_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEM8889639.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            9213..10382
                     /locus_tag="U2R41_002259"
     CDS             9213..10382
                     /locus_tag="U2R41_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8889640.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            10557..11936
                     /locus_tag="U2R41_002260"
     CDS             10557..11936
                     /locus_tag="U2R41_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8889641.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            12217..12446
                     /locus_tag="U2R41_002261"
                     /pseudo
     CDS             12217..12446
                     /locus_tag="U2R41_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4201 a         2138 c         2734 g         3373 t
ORIGIN      
        1 gaaaaattga gtagataaaa agataagtta cctggtactc attctatgaa gggcaattaa
       61 tagacatgac aaatagtatg ctgcttttat gcaccataca acagcaacga ttcaattgtt
      121 tttggttgct gactgtcatg tgtatccctc caagtaaaat ttttattaac gatagcatga
      181 aaaaaaataa aagtcaaagg cttgaaagaa aaaaatattt cgatagcatc gtttacccgt
      241 gtttattttt ctgacaaata ggcatgagac cttaaaagat agagaatact ctagttttct
      301 ggccggttgt ctgatacaat aaagttgatt atgataaaga aaagaaggag caaaaataat
      361 gatctcagca agtgatttaa gagccggtat gactttcgta caagacggca aactgattaa
      421 agttctggat gcaagccacc ataaacctgg taaaggaaat acagttatgc gtatgaaact
      481 gaaagatgtc cgttcaggtg caacatacga cacaacgttc agaccagaag aaaaatttga
      541 aaaagcacat atcgacacaa aacctgtaca atatttgtac acgatggatg atacagcttt
      601 cttcatggat ttggaaacat atgagcaata tgaaattcct gtagaaacag tagaagaaga
      661 aatgaaatat atcctagaaa acatggaagt aaaaatccaa tttttcggta gcgaagtcat
      721 cggcgttcaa ttaccaacaa ctgtcgtatt acgtgttgtg gaaacacaac cttccatcaa
      781 aggtgcaaca gtcactggtt caggtaagcc agcgacaatg gaaactggct tagtagtcaa
      841 tgttcctgac ttcatcgaaa cagatgaatt gttggaaatc aatacagcag aaggttctta
      901 tgtaaaacgt gcgtcaaaat aattcgctaa cggattgata agaatttgaa aacagagctt
      961 gacctcttga aaggcagatc atcggatctg cctttcaaga ggttttttgt ggaatcagca
     1021 aattgaaaaa taaaaaagta tttgatttta tttatatttt gaaagatgaa tggcaaaagc
     1081 tagagaaaga aaaaactgca cttatttatg aagaaattga aatgattcat aaaattgaaa
     1141 atatccgaac tattccgaac tatattgaag cgaaaatatt ttttcatcac taaatagtgt
     1201 tgaatttatg tttttatgta taaatagctt ctaatagttc gggtattttg tttttataac
     1261 catttttatt cttattttta aaaaaatatt tcgaaatata ctagacaatt cagaaaattt
     1321 tgtttaatat tattccaata aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg
     1381 aaaaaaagtg aaaagaggaa tgaaaaaatg acagaattga taattggcgg agtcacagca
     1441 agcacattgg cagaaaagta taagacacct ctgtatgtgt atgatgaaga aaagatgaga
     1501 caaacgatgc tgtcattcaa gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt
     1561 tacgcctcta aagcattcca aacgattgag atgctgcatt tagttcgtga atatggattc
     1621 gggctagatg ttgtcagtgg tggggaaatc tataccgctt tgcaagctga ttttcctaga
     1681 gatactatct acttccatgg taacaacaag acgccagagg aactgatcta tgcacttgaa
     1741 aatgatttgt tgcatattgt tgccgataat ttgatggaag ttgaattatt agcagaatta
     1801 agccaacgct atttaaaaaa aatctgtatc atgcttcggt tgaatgtcgg agtagaagca
     1861 catactcatg aatatatcgt cactgcgcat attgattcaa aattcggcat gagctatgaa
     1921 agtaaagagt gccaacaagc ccttcagttg ataaaagaaa gcagctatct agagttagaa
     1981 ggatttcatg ctcatatcgg gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc
     2041 gacaaattga cgaattactt aaccgatttt gaccaacctc tttccttgaa tcttggcgga
     2101 gggtttggga ttcgttatac acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa
     2161 aaactgattg aatatacgga agaagctttg gaaaaacgaa aactgaccat tcgtcaatta
     2221 ctgatcgaac cgggtagaag tattgttgga gaagctggta caacactata cacgattggt
     2281 ttcatcaaaa agacaccaca taaaaaatat tactttgtag atggcgggat gacagataat
     2341 atccgtccag ctctgtatca ggcagaatac gattgtgatt tagcagcaaa actgtcggaa
     2401 gaaaaaacag agaaagtgac gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt
     2461 aaagaagctt atttgccgaa agcagaatcg ggtgatcttt tagctgttta ttccacggga
     2521 gcttatggat actcgatgag cagcaactac aatcgagcga cacgaccagc agttgttttt
     2581 gtcaataatg gccaatcaag actgattgtt cgcagacaag cattttctga tctactaagg
     2641 ggggaagttt cctatgatcc ttcataaata taatggctgc ggaaatgatt ttatcttatt
     2701 agattacgca gaagacacag attatagtca attagccgct tctttatgca caaaagaaaa
     2761 atatgatata gacggattga tcgccgtaaa aatcgatccg ttggaaatga tctattacaa
     2821 caaagatggc agccgcgcgc cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt
     2881 aaatgatcag aacatgatga atgaacagga attcaacgta gagacattag caggcacaag
     2941 aaaaattacg gtgttggatc cctcaccttt ttattgcagc gtagatatgg gagtgccaga
     3001 tgattctccg gaaaaaatca aagctaatca aacttatcca ataaaaaacc aggaaataga
     3061 gatcaatggt gaaaaagtaa cgatcactag tttattttta ggaacgattc ataccgttgt
     3121 ttttgttgaa aatgcgcgtg aggaagtctg gagaaaaaga ggtgaactga tctgtcatca
     3181 tcctttgttc aaagaaaaaa ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt
     3241 gatcgtccgt acctatgaaa gaggagtagg ttggacactt gcttgcggta caggttgctg
     3301 tgcctcttat gtggttgcta aggaaactgg gaaaatttca gcagatcagg tagttgttca
     3361 cttggaacaa ggagacttga tgatctctgg tgaggatgcg atacaaatgg ctggcccagc
     3421 agttcatgaa tggacaagaa tattggagga atgaagatga taaaaggttc gatcgtggcg
     3481 ctgatcacgc cgatgaatga agagggatca gtagattatg ctggtttgga aaaattgatc
     3541 caattccatt tagatgaaca aacagatgga ttattagttt taggtacaac aggagaatct
     3601 tctacgctca cacaatctga ggaggagcag atccttcaac tgacagtaaa gaaagtggct
     3661 ggccgtgtgc cggtgattgc aggagctgga acgaataata cgaaagaaac gattgaaaaa
     3721 gcgaagcatt ttgcttcttt gggggcagac gcacttttgg tcatcacccc ttactacaac
     3781 aaaacaagtg atgcaggact agctgctcat tttacagcaa ttgcagaagc ctcaccgatt
     3841 ccgcttattt tgtataatgt tccttctaga acaggcatgt cgattccaat ccatgtatta
     3901 gtaaatttag cagaacatcc taatatcatt ggtttgaaag aagcctcagg agatatggct
     3961 tatgtgatgg atgccgctcg gttgattgga gaagaattct tcctttacag tggaaatgat
     4021 gatttgatcc taccagtaat gagtgtagga ggaagcggtg taataagtgt atgggcgaat
     4081 atccaaccta aaatagtcca tgaattagta aaagatacgc aggacggaag atggcagcaa
     4141 gcaaaagaaa agcaattaaa tgcacttgaa ctgatccatg ccttgttcag tgagaccaat
     4201 cctattcctg tcaaagcagc catgtcatta ttagacttac cttccggacc tctacgcctg
     4261 cctttagttt ctctttcaaa agagaaaaag aaacaattag ctcagctttt gttaaaagaa
     4321 aggacggttg aaaaatgaaa atcggactga ttggcagtgg acagatggga aatagagtcg
     4381 aagaagttgc acaagaaaga ggagatacgg tccttctttt acggacggca cctaaagaag
     4441 caactacgct tgcttttacg gaactgccag aactgcttat tgatttttct catccagata
     4501 atttagagaa aatcctttcg tatagcttat catatcaact tccgttagtc attggcacga
     4561 caggttatac acaatcacag ttccaagaaa taaaagaaca tgccaaacat gtacctgtga
     4621 tgtatagtgc taatttttcg ttcggcatca tggtgatgaa tcaactaatc aagcaagcag
     4681 ctgcaatgtt gcaaggctgg cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag
     4741 atgcaccatc tggtactgca aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc
     4801 agcccgtata caactggcaa gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta
     4861 ttcgtgcagg ttctttacct ggtgaacatg atgtattgtt tgctgctact gatgaaatat
     4921 ttacgatcaa acatgaatct ttttctaatc gaatctttgc tgcaggcgca gtagaagcag
     4981 cggactggtt gaaagatcag tcaccaggtt attacaaatt agaagattta ttaatggaca
     5041 aaggggtgta aaaaacgtgg aagctcaaga aatcatccgc tacattcaaa cggcatctaa
     5101 aaagacgcct gtgaaagtct atctgaatgt aaccgagcca atcgaattta aagaaagtaa
     5161 agtttttggt gaaggaagca gcttcgttgt ttttggtgac tatgagacga ttgctccggt
     5221 tttagaagct gaatcagaaa aaatcattga aatcgaaatc gaaactgctg caagatacag
     5281 cgcagttcct ttgctagata ttaaaaaaat taatgctcgt atcgaaccag gtgctattat
     5341 cagagatcaa gtgtcaatcg gcaacaatgc cgtgatcatg atgggagcga ttatcaatat
     5401 tggtgcagta atcggtgaaa acacgatgat tgatatgggt gctgtcttag gtggacgagc
     5461 gacagtgggc aaaaactgcc atattggtgc aggagctgtc ttagcaggtg tgatcgaacc
     5521 agcatcggcc aaaccggtta tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat
     5581 tgttgaaggt gtccatatcg gaaaagatgc agtcgtggca gcaggagcag tggttttaga
     5641 agatgtggca gcagaaactg ttgtcggtgg aatacctgca cgtgtattaa aaataagtga
     5701 tgatcagaca aaagaaaata cagcattgat tgctgcgtta cgcgaattat agaggagatg
     5761 gagaaaaatg cgcgaaatag atacctgtat tgtgggggca accggattag tcggccaaac
     5821 gatgctcaaa gtgttagaag aatatgattt tccagtagga cgattgaaat tattagcttc
     5881 aaaacgttca gccggtaaag tttgtgtatt caagaaaaag aaacacactg tacaggaatt
     5941 gacagaaact tcatttgaag gatatgattt agcgctattt tctgcaggtg cttctgtatc
     6001 caaagagttt gcgccaatcg cacgaagtca gggactattt gtcatagata attcctcagc
     6061 atggcgggaa gattcggata ttccattaat cgttccagag gtcaatctgg aagactatac
     6121 attgaatcgg ctgattgcaa atccaaattg ttcaaccatt caagcggtac ttccgcttaa
     6181 agctttacaa gaagcctttg gtatccagcg agtcaattat tcgacctatc aagcagtttc
     6241 tggttccggg caaaaaggca ttgaagatct tcatcgtacg caaaatgacg aggaaccagt
     6301 tttgtatcct tataatatta gccaaacggt gattccggaa atcgatcagc ctttggaaaa
     6361 tgggtataca aaagaagaac agaaaatgat aaatgagaac cgtaagattc tgcatcagcc
     6421 tgctttgcca gtatcagcaa cctgcgtacg tgtgccaatc gaaaatggac atggagtctc
     6481 tgtggcagtt caactgcaaa aagagtttac gacggaagaa attagagagt gtctcagcca
     6541 gtttccaggg attcaattag aagatgatct agcagcacat aaatatccga cgagcattct
     6601 tgctagagga acagatatgg tttatgttgg gaggatcaga aaagacactt ctttagagaa
     6661 cggattgttg ttctatacga ctgccgataa catacgcaaa ggagccgctg cgaatgccgt
     6721 acagattgct gcagctttaa tgcagaaaat agcggaggtg gcaagatgag taatacgctg
     6781 aaagtagcga aattcggcgg aacatcgatg tctcatgaag agcaattcaa aaaagtccgt
     6841 acaattgttc tttcagatcc caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt
     6901 acgtcagatg atgacaaagt aacagactta ctttatttga tttatgctta ccttcagcat
     6961 catgttgatt ggacacccct ttggaaaagg atttgtgatc gtttcattca agtaagagat
     7021 gttcttgccc tgcagttacc tattgaagat gaactagcac gcatacagaa acaactggaa
     7081 gacgaaaaga taactgaaga ttatttagta agtagaggtg aatatctgac tgctcaactg
     7141 atggctgagt atcttggtta ccagtttatt gacgcaaaag acctgatttt ctttgactat
     7201 gatgggaaaa tcgatcagga aaaaacagca caggaaatca caaaagcatt taaaagatat
     7261 ccgaaaatgg ttgttccagg gttttacggg gcgaaccctg caggaaaaga aaaattattg
     7321 ggccgtgggg gaagcgacat taccggcgcg atactggcga agatgttgca agcggaaagt
     7381 tatgagaatt ggacggatgt ttcaggaatc atgatggctg atccgcgaat catcgatcat
     7441 cctaaaatca tcgaggaaat ctcttttaga gaactgagag agatggctta tatgggggct
     7501 aacgttcttc atgaagcagc tgttttccca gttcaagagg ctgatatccc catccaaata
     7561 aaaaatacca atgatccttt cgctcaaggg acaaaaatat cgaatcacga aatagaaaag
     7621 gaaaatggac tgacaggaat agctggacgc aaggattttc tttccatcac tttattcaaa
     7681 cgtcatatgt ccgatgaaat cgggtttatc tggaaagcaa tgagcatttt tgctaaacat
     7741 gagatatcaa tcgaacatat tccttcaggg atcgacaata tcggagtgat cgtctcagca
     7801 gaagcgattg ccgataagtt atttctcatc acgaaagaat taaaagaaac acttggcgtg
     7861 gaagaaatcg tcgtgatcga agacttagct ttgatctctg tcgttggggg tccgcataaa
     7921 gaattgattg gattatctgg gaaagtgtta tcgatattga acaaattaga aattcgcaca
     7981 tcgatcttat cacaaggagc acaggagttg aatctgatca tcggtgtacc gaataatcaa
     8041 tatgaaacag tagtgaaggg tatctatgaa gggatggtga atacggatgc tgaaagaata
     8101 tcgaatggca ctccatcaaa ttcctgaaat cgggtttcat gaattcaaga caaaaaaata
     8161 tttatatgaa caagtaaaaa acagcgaggc cgttatccat gaaatcggcg aaacaggact
     8221 actcctttat tttgataatc agcaagaaga aacgattgct tttcgaacag atatcgatgc
     8281 actgccagtc aaagaagaaa cgaatctctc ttttgcttct aagcatgaag gttatatgca
     8341 tgcatgtggt catgacggac atatggcgat gcttttaggg ttgactgact ttataactaa
     8401 acacattgag gatcttattt ataatgtagt actgatctat cagccatcag aagaaatttc
     8461 cggtggcgca caaagcgtta ttcagtcagg attgttggat aaatatgaag tacaagcaat
     8521 atttggcttt catctatggc ctggactagc taagggagaa gttttctctc gaccaggacc
     8581 tttgatggca caaagcagtg aaacagacat tatcattaaa ggccagtctg cacatattgc
     8641 ttctagtgaa aaaggaatcg atagtctgga ggcagctgta cgatttttga agaatgttta
     8701 tgattttgac cggcaattac cggcagagtg gaatcatcta atgaaatttg gccaaatcca
     8761 aggtggaacg atccgaaatg ttttagctgg agaagttatt gtatccggaa gtatccgttc
     8821 ttacagttca gatatgcaaa attatttgaa gaggggatta gaccatttag cagaacagtt
     8881 tcactctaca gctactattt cttttcgcta taatgacggc tatccagctg taatcaacca
     8941 tagaggctta tatgcagcac tgttagattc aaacaaacta catgaattag atgctccagt
     9001 tcttcaagcc gaagattttg gcatatatac gcaacaatat ccatgtgtat ttttcttttt
     9061 aggtgtcggt gatacccctg ctttacatga tccgacgttc gattttgata tggatgtttt
     9121 agaaaatggt gtagaatggt acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat
     9181 cgatagcagt tcaaaagagg agcgagtaaa taatgatgaa tttatccaat cgatttagtc
     9241 ctaggctttc tagaatagaa gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga
     9301 taccagatgt gatcaaactc accctgggag aaccggattt tccaactcct gaacatgtca
     9361 agcaagcagg aatagcagcg attgaagaag acttttctca ctataccgga atgagaggat
     9421 tagaggaact gcgggaagcg gcttgtatat ttcagcaaca gcgttatgga ttaacttatg
     9481 atccgcaaac ggaagttttg acgacagttg gtgcaacgga agcgattgcc tctgcgttgt
     9541 tatctgtttt ggaagaagga gacaaggttc ttattccagc gcctgcttat tcaggatatc
     9601 agccattagt ggaactagct ggtgctgaat tgatcccgat agatacttct gacacagggt
     9661 ttgtctgtca gccggaacaa tttgaacgag catttgaaca atatggatca gctgtcaaag
     9721 cagtcatttt aaactatcct aacaatccta ctgggacgac attgtctgca aaacaattga
     9781 aagcaatcgc tgaggtttta aaaaaatatc cggtatttgt tataagtgat gaagtatatg
     9841 ctgaattgac ttatagtgga acgcatatgt ccatagctac ttatcttcca gaacaaacaa
     9901 tcgtgataag cgggttatcc aagtcacatg cgatgactgg ttggcgagtg ggctttattt
     9961 ttgctcaaaa agcccttatt gacgaactaa ttaaagtcca tcaatatctg gtgactgccg
    10021 ctacaacgat gtcgcaaaaa gctgcggctg aagcgctaat aaatggggtg aaagatagcg
    10081 agaatatgaa agagcaatat caaacacgaa gagactatct ggtccagcaa ttggcgcctt
    10141 taggatttga agtgacccag ccaaacggtg cattttactt gttttgcaaa ctcccagctg
    10201 ccattcaaac tgactcttgg cagttttgtg tagaattagc ggaacaggca aaagttgcgt
    10261 gtatcccagg aattgcattt ggcccagaag gagaaggtta tatccggatt tcttatgcta
    10321 gcagcatgga aaaattacat gaggcctgca aacggatcaa agacttttta acagaacagt
    10381 gattcataga ttttatcaaa aaaacaatcg ttaattttgc ggtatattct gcaaggagac
    10441 gattgttttt taaatttagt tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat
    10501 gttaagttta aattgtttag aatgatttta aaagaatatt caaggaggtt tcatcaatgg
    10561 aaaaaatcaa agttgtcatt gtaggtgcat ctcatggagg gcatcaatcg atcttggaac
    10621 tattgacacg atacggtgaa aatgtagata tcacgctatt tgaagcagga gactatattt
    10681 ccttcatgtc atgtggtatg gagctgtatt tagaagacaa ggtaacagat gtaaatgatg
    10741 tacgtaattt cagaccagaa aactttccgc aatccaatgt tcatatttta aataatcacg
    10801 aagtaaaaac gatcgatgcc gataaaaaaa cagtaacggt tactagcgca tcagataacg
    10861 aaacaaaaga atatccttat gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc
    10921 tgccagtacc aggagcagat ctagaaaatg tctacttgat gcgtggatac aattgggcaa
    10981 ccaaaatcaa agaaaaatta aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg
    11041 gttacattgg gatcgaagca gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc
    11101 ttgatgtgat cgatagacct ttaggcactt atttagatgc tgagatgaca gatattttag
    11161 aacaacacct aaatgaaaac ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta
    11221 ctggtaacgg aaaagttgag gcaatcaaaa caagtgaaaa agaaatccca gcagatctag
    11281 tcattcaagc tgccggtgtg aaggcaaata cagaatggtt aaaagggatc gtcgacttag
    11341 atgaacgtgg ctggatccaa acaaatgaat acttgcaaac gaatcttcca gatgtgtatg
    11401 cagtaggaga tgctacactt gcttattcta tcccagcacg caaaaaaatg ccgatcgctt
    11461 tagcgactgt tgcacgtaga gaagcacgtt atgtagtaaa acatcttttt gaagaagtac
    11521 caaacaaacc gttcggcgga gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt
    11581 ttgcagcaag cggattgaat agctttacag cagcaaaagc aggagtagat gtacgagtgt
    11641 cttattatga agatacgtta cgaccaaaat atgtaccaga agaacaagga aatccaaaag
    11701 tgtccatcca attattctcg gatccaatga cccatcaatt gctaggagga gcagtattat
    11761 ctacctatga tgttacaggt caaggaaatg tattggcact agcgatccaa aacaaactga
    11821 ctttagaaga actagcagaa gctgacttct tcttccagcc aggctttgac cgacaatgga
    11881 gcatgttgaa tctagccgct caacaagcgt taggagaaga accatttgta gaataagaaa
    11941 acgaaatatt tgaaaagagg ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc
    12001 aagtaaaaac agcttcttat gtttttaact tggctggact tattaccgca gatgcaagct
    12061 tttgaacacc gtttattttc aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa
    12121 aagttagacc aaaaaatcta acttttggag gtcggtattt tttatggtta aatatacttt
    12181 tgaacttaaa ttaaagatcg ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa
    12241 aatccagatc aagagattga agcggaaatg cttaaaattc gggagaagca caaggattat
    12301 ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa
    12361 gttcagcgct tgatcaagaa gctaggaata aaagtaacgg catacacgcg taaatcacgt
    12421 cgctacaatt cttatcgagg aaaagt
//
LOCUS       DASHWH010000074        12246 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.077, whole genome shotgun sequence.
ACCESSION   DASHWH010000074 DASHWH010000000
VERSION     DASHWH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.077"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2R41_002262"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2R41_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEM8889642.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2R41_002263"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2R41_002263"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEM8889643.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="U2R41_002264"
     CDS             complement(2169..2906)
                     /locus_tag="U2R41_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM8889644.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="U2R41_002265"
     CDS             complement(2997..3524)
                     /locus_tag="U2R41_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889645.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="U2R41_002266"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="U2R41_002266"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEM8889646.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="U2R41_002267"
     CDS             complement(4401..4886)
                     /locus_tag="U2R41_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEM8889647.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="U2R41_002268"
     CDS             complement(5039..5236)
                     /locus_tag="U2R41_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889648.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2R41_002269"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2R41_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEM8889649.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2R41_002270"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2R41_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEM8889650.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="U2R41_002271"
     CDS             9211..9423
                     /locus_tag="U2R41_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEM8889651.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="U2R41_002272"
     CDS             9425..11104
                     /locus_tag="U2R41_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM8889652.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="U2R41_002273"
     CDS             complement(11358..11558)
                     /locus_tag="U2R41_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8889653.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="U2R41_002274"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="U2R41_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3575 a         2636 c         2117 g         3918 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaat
//
LOCUS       DASHWH010000075        12175 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.078, whole genome shotgun sequence.
ACCESSION   DASHWH010000075 DASHWH010000000
VERSION     DASHWH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.078"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(137..301)
                     /locus_tag="U2R41_002275"
     CDS             complement(137..301)
                     /locus_tag="U2R41_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889654.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            596..1924
                     /locus_tag="U2R41_002276"
     CDS             596..1924
                     /locus_tag="U2R41_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEM8889655.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="U2R41_002277"
     CDS             2126..2959
                     /locus_tag="U2R41_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEM8889656.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="U2R41_002278"
     CDS             2975..3712
                     /locus_tag="U2R41_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEM8889657.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3867..4835)
                     /locus_tag="U2R41_002279"
     CDS             complement(3867..4835)
                     /locus_tag="U2R41_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEM8889658.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="U2R41_002280"
     CDS             5067..5900
                     /locus_tag="U2R41_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEM8889659.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="U2R41_002281"
     CDS             5934..6773
                     /locus_tag="U2R41_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8889660.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="U2R41_002282"
     CDS             6799..7359
                     /locus_tag="U2R41_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM8889661.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="U2R41_002283"
     CDS             7558..9279
                     /locus_tag="U2R41_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEM8889662.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="U2R41_002284"
     CDS             9444..10586
                     /locus_tag="U2R41_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8889663.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="U2R41_002285"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="U2R41_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEM8889664.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3455 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat tgtttctcaa
     3781 atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag cctctctttt
     3841 taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa ttcttcaccc
     3901 gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact attaatactg
     3961 ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata atcataaaca
     4021 ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt ttggacaaga
     4081 agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa agcttcgatt
     4141 accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg catcccagca
     4201 tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg ctttgtttct
     4261 aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg acccaatgta
     4321 ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg tgcagaatgg
     4381 atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca acttgcgaca
     4441 cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact tgaacgcatt
     4501 gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa aaaagcagtt
     4561 tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga tgcataagcg
     4621 gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg tgagggaaga
     4681 ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc tgcatctaat
     4741 aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt catggcgatc
     4801 gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttg
//
LOCUS       DASHWH010000076        11893 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.079, whole genome shotgun sequence.
ACCESSION   DASHWH010000076 DASHWH010000000
VERSION     DASHWH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.079"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(94..969)
                     /locus_tag="U2R41_002286"
     CDS             complement(94..969)
                     /locus_tag="U2R41_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM8889665.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="U2R41_002287"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="U2R41_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEM8889666.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="U2R41_002288"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="U2R41_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEM8889667.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="U2R41_002289"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="U2R41_002289"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEM8889668.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="U2R41_002290"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="U2R41_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEM8889669.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="U2R41_002291"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="U2R41_002291"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEM8889670.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="U2R41_002292"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="U2R41_002292"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEM8889671.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="U2R41_002293"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="U2R41_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEM8889672.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="U2R41_002294"
     CDS             complement(9420..10187)
                     /locus_tag="U2R41_002294"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEM8889673.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="U2R41_002295"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="U2R41_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEM8889674.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="U2R41_002296"
     CDS             11300..11698
                     /locus_tag="U2R41_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8889675.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3138 a         2582 c         2081 g         4092 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    11761 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    11821 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    11881 cattggtgat ttt
//
LOCUS       DASHWH010000077        11759 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.080, whole genome shotgun sequence.
ACCESSION   DASHWH010000077 DASHWH010000000
VERSION     DASHWH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.080"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            251..526
                     /locus_tag="U2R41_002297"
     CDS             251..526
                     /locus_tag="U2R41_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889676.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2617
                     /locus_tag="U2R41_002298"
     CDS             539..2617
                     /locus_tag="U2R41_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEM8889677.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2777..4663
                     /locus_tag="U2R41_002299"
     CDS             2777..4663
                     /locus_tag="U2R41_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8889678.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4675..5622
                     /locus_tag="U2R41_002300"
     CDS             4675..5622
                     /locus_tag="U2R41_002300"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEM8889679.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5642..6169
                     /locus_tag="U2R41_002301"
     CDS             5642..6169
                     /locus_tag="U2R41_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8889680.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6232..6885)
                     /locus_tag="U2R41_002302"
     CDS             complement(6232..6885)
                     /locus_tag="U2R41_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEM8889681.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7017..7859
                     /locus_tag="U2R41_002303"
     CDS             7017..7859
                     /locus_tag="U2R41_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8889682.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8017..8913
                     /locus_tag="U2R41_002304"
     CDS             8017..8913
                     /locus_tag="U2R41_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8889683.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8916..9629
                     /locus_tag="U2R41_002305"
     CDS             8916..9629
                     /locus_tag="U2R41_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEM8889684.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9649..10167
                     /gene="msrA"
                     /locus_tag="U2R41_002306"
     CDS             9649..10167
                     /gene="msrA"
                     /locus_tag="U2R41_002306"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM8889685.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10164..10391
                     /locus_tag="U2R41_002307"
     CDS             10164..10391
                     /locus_tag="U2R41_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEM8889686.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10543..11094
                     /gene="lepB"
                     /locus_tag="U2R41_002308"
     CDS             10543..11094
                     /gene="lepB"
                     /locus_tag="U2R41_002308"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8889687.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11177..11677)
                     /locus_tag="U2R41_002309"
     CDS             complement(11177..11677)
                     /locus_tag="U2R41_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM8889688.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT         4101 a         1997 c         2400 g         3261 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt
     2641 ataaaaaaca aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac
     2701 aaaaagaaca aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc
     2761 aagataagga gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg
     2821 tgaaaaagat ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt
     2881 gattgggacg aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag
     2941 cagcgacggt gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc
     3001 tcaagcagct gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag
     3061 ccccttgatc caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg
     3121 agaagaccct gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga
     3181 tgcttggact acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt
     3241 agataaaaaa atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt
     3301 gttgattgat gaacccgatt tactattact agacgaacca acaaaccatt tagattatga
     3361 agcgatcgag tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac
     3421 ccacgatcgt tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa
     3481 attatacgag tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg
     3541 tgtagaagta gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat
     3601 gagggctggc gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga
     3661 tctaaaggag aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac
     3721 acaacgttta gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca
     3781 aacactgcta gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg
     3841 taaaaatggt gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga
     3901 tagcggtaca atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga
     3961 agaaatggca ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa
     4021 aacagcagat ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag
     4081 atttatgcat gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt
     4141 gttgaaactg ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt
     4201 ggacatagca acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat
     4261 cactgtttcc catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca
     4321 aggaaacgga aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa
     4381 agagacccag cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga
     4441 aaagaagaaa ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc
     4501 agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt
     4561 cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa
     4621 aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa
     4681 caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga
     4741 acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg
     4801 tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg
     4861 tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat
     4921 gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat
     4981 tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag
     5041 cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga
     5101 cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa
     5161 atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct
     5221 gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa
     5281 ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt
     5341 ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa
     5401 acaggtttag cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat
     5461 catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt
     5521 ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga
     5581 tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa
     5641 aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct
     5701 tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact
     5761 agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac
     5821 gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt
     5881 ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc
     5941 agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca
     6001 tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa
     6061 tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca
     6121 gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg
     6181 atagactaga tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa
     6241 gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac
     6301 atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc
     6361 gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc
     6421 cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg atactctctc
     6481 ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa
     6541 tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact
     6601 tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt
     6661 tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc
     6721 atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag
     6781 aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac
     6841 ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt
     6901 actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct
     6961 tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga
     7021 caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt
     7081 taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg
     7141 accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa
     7201 caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca
     7261 gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac
     7321 aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag
     7381 gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga
     7441 aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca
     7501 cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg
     7561 ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg
     7621 ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat
     7681 tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat
     7741 tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc
     7801 cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt
     7861 cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa
     7921 aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata aaatttttga
     7981 taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg
     8041 atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct
     8101 ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa
     8161 aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa
     8221 accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg
     8281 accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag
     8341 cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg
     8401 actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc
     8461 aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa
     8521 tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat
     8581 ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca
     8641 gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat
     8701 caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg
     8761 acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca
     8821 aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa
     8881 aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt
     8941 caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag
     9001 tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc
     9061 cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata
     9121 aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata
     9181 ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag
     9241 gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc
     9301 aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga
     9361 atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca gatgatctga
     9421 ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata
     9481 aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg
     9541 atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa
     9601 aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca
     9661 atatttgcag gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc
     9721 catacagtga tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta
     9781 aaaagccaaa cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt
     9841 tcatatgaac aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga
     9901 caattcgagg atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag
     9961 cggatcattg ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca
    10021 atcgttacaa aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag
    10081 tattataaaa agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc
    10141 gaagaaaatt ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc
    10201 gaggcggtgg cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc
    10261 aatttcctaa gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg
    10321 actatttgcc gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc
    10381 atgacatata agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct
    10441 aattgaaaga attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat
    10501 aataaagaaa gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc
    10561 tatattaatc gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc
    10621 atgttgcggg gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg
    10681 ttgaagcaag gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt
    10741 gttgtttttc aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga
    10801 gaaaatgtca gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct
    10861 tatctaacta aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca
    10921 ctacaagagc taacgggaca atccaagctt ccggaagata attattttgt acttggagat
    10981 aatcgaagag tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta
    11041 ggtaaagcaa gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca
    11101 ataaagcgtg cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact
    11161 accttagtac gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga
    11221 taaatgaata aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac
    11281 ctagaaaaat tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa
    11341 aaaggctgac tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct
    11401 ttttatgata taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag
    11461 cagcttgaaa cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa
    11521 gcaaaccagc ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga
    11581 atgtgatttg aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta
    11641 ataaaagagc agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt
    11701 ttgcaatcaa agtgttcaaa atattctctc ctttgtcagt taataataaa aggatacca
//
LOCUS       DASHWH010000078        11639 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.081, whole genome shotgun sequence.
ACCESSION   DASHWH010000078 DASHWH010000000
VERSION     DASHWH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.081"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            235..1875
                     /locus_tag="U2R41_002310"
     CDS             235..1875
                     /locus_tag="U2R41_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEM8889689.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="U2R41_002311"
     CDS             2029..3351
                     /locus_tag="U2R41_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEM8889690.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="U2R41_002312"
     CDS             complement(3413..3844)
                     /locus_tag="U2R41_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889691.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="U2R41_002313"
     CDS             complement(3908..4744)
                     /locus_tag="U2R41_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEM8889692.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="U2R41_002314"
     CDS             complement(4934..5236)
                     /locus_tag="U2R41_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEM8889693.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="U2R41_002315"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="U2R41_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEM8889694.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="U2R41_002316"
     CDS             complement(5690..5956)
                     /locus_tag="U2R41_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEM8889695.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="U2R41_002317"
     CDS             complement(6210..6935)
                     /locus_tag="U2R41_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEM8889696.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="U2R41_002318"
     tRNA            complement(7301..7371)
                     /locus_tag="U2R41_002318"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="U2R41_002319"
     CDS             7488..8240
                     /locus_tag="U2R41_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM8889697.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="U2R41_002320"
     CDS             8256..8735
                     /locus_tag="U2R41_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM8889698.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="U2R41_002321"
     CDS             complement(8986..10566)
                     /locus_tag="U2R41_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEM8889699.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="U2R41_002322"
     CDS             complement(10559..11302)
                     /locus_tag="U2R41_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889700.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..>11639)
                     /locus_tag="U2R41_002323"
     CDS             complement(11478..>11639)
                     /locus_tag="U2R41_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEM8889701.1"
                     /translation="DLKAYIINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFM
                     QEKIENESF"
BASE COUNT         3538 a         2319 c         2064 g         3718 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatc
//
LOCUS       DASHWH010000079        11235 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.082, whole genome shotgun sequence.
ACCESSION   DASHWH010000079 DASHWH010000000
VERSION     DASHWH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.082"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            80..1126
                     /gene="gmd"
                     /locus_tag="U2R41_002324"
     CDS             80..1126
                     /gene="gmd"
                     /locus_tag="U2R41_002324"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEM8889702.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1206..2219
                     /locus_tag="U2R41_002325"
     CDS             1206..2219
                     /locus_tag="U2R41_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889703.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2256..3200
                     /locus_tag="U2R41_002326"
     CDS             2256..3200
                     /locus_tag="U2R41_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEM8889704.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3268..4581
                     /locus_tag="U2R41_002327"
     CDS             3268..4581
                     /locus_tag="U2R41_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEM8889705.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4665..5567
                     /locus_tag="U2R41_002328"
     CDS             4665..5567
                     /locus_tag="U2R41_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEM8889706.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5572..7224
                     /locus_tag="U2R41_002329"
     CDS             5572..7224
                     /locus_tag="U2R41_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEM8889707.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7248..8114
                     /locus_tag="U2R41_002330"
     CDS             7248..8114
                     /locus_tag="U2R41_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889708.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8156..8347)
                     /locus_tag="U2R41_002331"
     CDS             complement(8156..8347)
                     /locus_tag="U2R41_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889709.1"
                     /translation="MIIRDDHIYTCDSCHYSFPTDEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8377..8916
                     /locus_tag="U2R41_002332"
     CDS             8377..8916
                     /locus_tag="U2R41_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889710.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9044..9397
                     /locus_tag="U2R41_002333"
     CDS             9044..9397
                     /locus_tag="U2R41_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889711.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9864..10340
                     /locus_tag="U2R41_002334"
                     /pseudo
     CDS             9864..10340
                     /locus_tag="U2R41_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10340..10534
                     /locus_tag="U2R41_002335"
     CDS             10340..10534
                     /locus_tag="U2R41_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889712.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10540..10825
                     /locus_tag="U2R41_002336"
                     /pseudo
     CDS             10540..10825
                     /locus_tag="U2R41_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10839..11205
                     /locus_tag="U2R41_002337"
                     /pseudo
     CDS             10839..11205
                     /locus_tag="U2R41_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3152 a         2302 c         2855 g         2926 t
ORIGIN      
        1 tcgtgtattc ttgaaaagta ttagaaatta cgagccattt atatggtctt atcacttaca
       61 taggaaagga taatgtatca tgaaaaaagc gctgattact ggtattactg gtcaggacgg
      121 ttcctatctg gcagatttcc tgctagagaa gggctacgaa gttcacggta tcactcgccg
      181 tgcttctatt tccaataccg ctcgcattga ccatttgatg ggcaagatca cccttcatga
      241 cggtgacctg tccgactcat cttctctgat tcgtatcatc agcattgttc agcctgatga
      301 aatctataac ctcgctgcac agagccatgt tcaggtttct tttgatgttc ctgagtactc
      361 cggcgatgtt gatgctttgg gcgttatgcg tattctggaa gcttgccgta ttctgggcct
      421 gacgaagaag actaagattt atcaggcatc tacttctgag ctgtatggca aggttgaaga
      481 agttcctcag cgtgaaacca ctccgtttca tccttacagc ccttatgctg ttgcaaagca
      541 gtatggcttc tggatcacta aggaatatcg tgaagcttat ggcatgttcg ctgtcaacgg
      601 tattctgttc aaccatgaat ccgaacgtcg cggtgagaat ttcgtcaccc gtaagatcac
      661 cctcgctgcc ggccgcattg cagaaggcct gcaggaccat ctggaactgg gcaacatgga
      721 ctccctgcgt gactggggct atgcaaagga ctatgttgag tgcatgtgga tgattctcca
      781 gcatgagact cccgaggact tcgtgatcgc taccggcgag cagcacactg ttcgtgactt
      841 caccgagaag gcattcgcag caaatggcat gaccattcgt tgggaaggca ctggcattga
      901 tgaaaagggc tacgatgttg aaaccggcaa gatgctcgtt tgtgtaaatc cacagtggtt
      961 ccgtccgact gacgttgaca acctgtgggg cgacccgact aaggcaaaga ccgttctcgg
     1021 ttggaatccg cagaagacct cttacgagga actggttaac atcatggcaa agcatgaccg
     1081 tgaattggca aagcgcgaag ctgcaatgaa gaacgcagtc aagtaagctg acattgaata
     1141 aacatatctg tttctatctt tccagtcacg gcttcggaca tatcgcgcgg aatctgccca
     1201 ttattatggc aacactctgc cgatgcgaag cgaagattac actggtttgc ggcgcaaatc
     1261 atctggattt tgcaaagcgc aatttggagc aggaactgcc caaagagctg atagaccgcg
     1321 tgcgctatcg tccaataaac acagatatcg gtctgattaa ccgtgagggc accctactgg
     1381 tggacacggg taagctgacc gagatctgtg aatatttttt ggcaaaggtt ccttctttgg
     1441 caaagacaga agccgaatgg ctcaagcgag aagctgtcag cgcagtgctt tgcgatatgc
     1501 caatctgggc aatggatgcc tgcgatatgg caggagtacc tttcctgtat gtaggaaact
     1561 tcacttggac agagctgtat cgcgaatttt tacccgaaca tatttggaaa gaatatgcca
     1621 agcgttacgg taaaattcag cacaccatgc tttatgcgct aaataacgaa gaaatgttgg
     1681 aatttctcca aaacagcgaa aaaacaccga cttccgttgt ggcgcgtccg attcataacg
     1741 acgaagtgca acgcattaaa tcacagcatg aaaaaccgcc tgtatttgtc gcactcggta
     1801 tgtccgcgcg gttcaaacag ccaattgatg tcagtcacgc gccgtatgat ttcattacaa
     1861 cgctaggtgt tccgcttgtg ggagaaaacg taaccgttat ttctaacagc acccccaata
     1921 cacaggacta tatagcggcg gcagattacg ttattacaaa ggctggatgg ggaactgttg
     1981 cggagtgtat tctcgcacag cgtccgatgg cactgttcgc ccgcgataca gttttggaag
     2041 atagaacaac tatttgtaaa ttgcaaaaac tgggtctggc aatttccgtc acaccggaaa
     2101 acctccaccg cgtagagcgg gtgattgagg acctcaaaca gctagacacc tcaaattttg
     2161 atacatttta tgatgctgcg agcgaaatcg cagacaaatt gatttctctc atgcaatgag
     2221 agatgctcta aatttagaaa ggagcaagca ttattatgat ggaaaaagac gcaaaaatct
     2281 acgtagccgg tcatcgtggc atggctggtt ccgcaatcgt gcgcgagctg aaccgtcagg
     2341 actacaacaa tattatcacc cgcacccata aggagcttga cctgtgccgt caggacgctg
     2401 ttgaagcatt tttcgcacag gaaaagccgg attatgtatt tctcgctgct gcaaaggttg
     2461 gcggtattat cgcaaaccag aacgctctgg cagacttcat gtatgaaaac atgattctgg
     2521 aaatgaacgt catcaactcc gcatggaaga atggctgtaa gaagttacag ttcctcggtt
     2581 cttcctgtat ctatccgcgt atggcaccgc agccgatgcc ggaaagctgt ctgctgacaa
     2641 gtgagctgga aaagaccaac gaggcttatg cactggcaaa gatttctggc ctgaagtact
     2701 gtgagtttct aaacaagcag tacggtactg actatatctc tgttatgcct actaacctgt
     2761 atggacctaa cgacaactat catcctactc acagtcatgt gctccccgct ttgattcgtc
     2821 gcttccatga ggctaaggaa gcaggtctgc ctactgttac ctgctggggt gatggttctc
     2881 ctctgcgcga attcctatat gttgacgatc tggcaaacct gtgtgtgttc ctgatgaata
     2941 actatagcgg tgacgagact gttaatgcag gtactggcaa ggaactgtct atcaaggaac
     3001 tgaccgagat ggtagcaaag gttattggtt acgaaggcga gattctgtgg gataccagca
     3061 agccgaacgg tacacctcgt aagctgctgg atgtttccaa ggcaaccaag ctgggatgga
     3121 catataagac tgagttggaa gatggcatcc gtctgtccta cgaggacttc ctgaataacc
     3181 cgatgcgtgc tgaacgctaa tttaccgagg ttgctgtgat ttttgaggat tgcagcagcc
     3241 tctttcttat aaaaaggaga gatcattatg aatattgttt tactgtctgg cggttctggt
     3301 aagcgccttt ggccgctgtc taatgacatc cgttccaagc agttcattaa gattttcaaa
     3361 actgaagatg gatatgaatc catggttcag cgtgtctacc gccagattaa gaaggtagat
     3421 aacgacgcaa ctgttacgat tgcaacctct aagacacagg tttctgctat ccataaccaa
     3481 cttggtgagg atgtgggtgt atctgtagag ccttgtcgcc gcgatacctt cccggcaatc
     3541 gcattggcta ccgcttatct ggtagatgtg atgggcattg atccagaaga gtccgtagtt
     3601 gtttgcccgg ttgaccctta cgtagaagat gactattttg aggccctgaa atctctgtcc
     3661 gatcaggctg acaaaggcga agctaatctg gtgctcatgg gtattgaacc gacttatcct
     3721 tccgagaagt acggctacat cattcctgag agcgcagagc agactgcttt cgtaaatacc
     3781 ttcaaggaaa aaccttctga ggctgtggca aaggaatata tcgcacaggg tgcactgtgg
     3841 aacggtggtg tgtttgtcta caagctgaag tatgtcatgg agcgtgcaca tcagctgatt
     3901 gactttgtag attatcagga tctgttcaac aaatacgata ctctgactaa gatttccttc
     3961 gactatgccg ttgttgagca tgaggacaag attcaggtac agcgttttgc tggtatgtgg
     4021 aaggatctgg ggacttggaa tactctgacc gaaggtatgg aggaatccat tatcggtaaa
     4081 ggtgttatga atgagaattg cgatggcgta cacatcctga acgaactgga tgttcctgtg
     4141 ttggctatgg gtctgcatga tgtggtcatc tctgcatctc gggaaggtat tctggtttcc
     4201 gataaggaac agtccagcta tattaagcct ttcgttgaca agttcgagca gcagatcatg
     4261 ttcgcagaga agtcttgggg tagttataag gttatggatg tagaagcaga gagcatgacc
     4321 attaaggtta ctctgaatgc tagtcactcc atgaattatc acagccacaa gaaccgtgac
     4381 gaggtgtggg tggttctggc cggtactggt aagaccatcg tggatggata tgagcaggtt
     4441 gttaaggctg gcgatgtgat caccatgagt gctggctgtc ggcataccat tatggcagat
     4501 actgagctga agctgattga agtacagctt ggcaaggata tcaatgttaa agataaacag
     4561 aaatttgagt tagagtatta aaaaacggag gagggctcgt tatacagaaa ccgtttttat
     4621 tgaagccttc cgcaaaggat tatctgtggg ggggaaccgc ctgaatgatg aatttgcaaa
     4681 aggaattgag atgtctcctc ttgcggaaac ttggaatgct ctacgcaccc ggatggaccg
     4741 agctttgttg tgtctggtca gagtatcagg ggatgacctt gacggaggtg ttgcaagaac
     4801 acccagagtt tctgggaacg catccgcaga ccaaaggaga attgccgatc ctgatcaaat
     4861 tcattgatgc gaaaaaggat ttgtctgttc aggtacatcc cacagatgaa tatgcaattg
     4921 aaaaagaaaa cgggcagctt ggaaagacag agatgtggta tgtactggat gccagcttaa
     4981 tctatgggct gcagaaaata acagaagaag ctgtgatccg tgaagcgatt gctcagggaa
     5041 ctgttgagcg ttatcttcag aaggttccgg tcaaaaagaa tgatgtgttc tttattgagg
     5101 cgggaacgat tcatgcgatt ggtgcgggtg cactaattgc agaaattcag gaaaattcca
     5161 atctgactta tcgtctctac gattatgacc gtgttgacaa aaatggtaaa aagagagaac
     5221 ttcatgtaga caaagcgcta gaagtagcga acttgaaagg gttgtcagaa ccgaaacagc
     5281 cgcttcgtgt tctaaaatat cgtgctggtg tagctgcgga attgctttgc agatgcaagc
     5341 atttcgaggt ctctcgtatg attgtcaaca ctgagagaag acagacggta cactatcgtg
     5401 cggattcagt cagcttccgt gtgttgctgt gtgtcaatgg ctgtggtacc gttcgatttg
     5461 ataatgaatc gttggacatc tataaggggg actgcctttt tgtgccggcc gattctactg
     5521 aaatcagcat tcacgggtcg attcagtttc tggatgtgag aggataataa tatgaattat
     5581 aaagtagaat atgagcgttg gctgaatcat gccaatgcag atattataga agaactgcgt
     5641 tcctattctg agaaggacat tgaggactct ttctataaaa atttgtcttt cggtactggt
     5701 ggtctgcgtg gtaccatcgg tgcaggcact aatcgcatga acgtacacac cgtaggtaag
     5761 gcaagccaag gtctggcaga ctatctgaat aagaactatg aagccgcttc cgtggtggtt
     5821 ggctatgatt cccgtatcaa atctgatgtg tttgcaaaga ctgccgcaga catctttgct
     5881 gcaaatggta ttcaggtgca cttgtggcca gaactgcttc ctgttcctac agtatctttt
     5941 gcgactcgtt atctgaaggc agatgccggt gttatgatta cggcatctca taacccttct
     6001 aaatataacg gctacaaggt atacggtgca gacggctgtc agattactac cgaggctgct
     6061 actgcaattc tggcagaaat cgaaaagctg gacatctttg ctgatgtaaa gatggatggc
     6121 gatgcttcta agattgcatc cattcagtcg gaagttctga ctgctttcat cgaagaagtg
     6181 aaggggcagt ccgtcctgtt tggcgaaaaa gtggacaaga atgttgctat cgtgtattct
     6241 cctctgaatg gtaccggtct ggtgcctgtt acccgcaccc tggcagagat gggatacagc
     6301 aatgtgaccg ttgtggaaga acagcgtctg ccggacggta atttcccgac ttgtccttat
     6361 ccgaaccctg aaattaagga agctatgtct ctgggtatgg agtatgccaa gaagtacaat
     6421 gcggatctgc tgttggccac tgaccctgac tgcgaccgtg ttggtatcgc agttaagagc
     6481 gcacatggtg actatgttct gttgtctggt aatgagactg gtctgctact tctggactac
     6541 atctgttctc agagaattaa gcatggaaaa atgcctgctg accctgtgat ggtcaagacc
     6601 atcgtcacca tggatttggg tgaacgtatc gcatccaact atggtgttcg taccatcgat
     6661 gtgctgaccg gctttaagtt tatcggtgag cagattggta tgttggaaaa ggctggtaag
     6721 gctgacagct atatcttcgg ctttgaggaa tcttacggct atctgtccgg tagctatgtc
     6781 cgtgacaagg acggtgttaa cgctgcttat ctaatctgtg aaatgttctc ttactacaag
     6841 actcagggta tcagcctgct ggataagctg aatgagattt atacaaagta tggctattgt
     6901 ttgaacaccc tgcattcctt cgagtttgac ggtgcggcag gttttaccaa gatgcaggaa
     6961 attatggttg aattccacaa gggtctcgat ataattggtg gtaagaaggt tattgaaact
     7021 ttagactaca gtaagggcct gaatggtctg ccgaagtctg atgttttgaa gtttatgctg
     7081 gaagataact gctctgttgt ggttcgtcct tctggtactg agccgaagct gaagacctat
     7141 atcagcgtca gcgcagagaa caaggaagcc gccgaagcta tggaagctat tatggctgct
     7201 gaactggcgg aaatgttcga gtaagaaaag aaaggagaaa tcttgtaatg gaggaactgt
     7261 tttctaacgc tttgaaatcg gtgaagtcaa atcgtgtgct gtacacggca tcgttctttg
     7321 caagatcctc tcttttgtat ttgcaggaaa ttggacagct gactgctctg aaggcacata
     7381 catctaggcg tacaggattg tcatcttacc tttgtttcct ggtagaagtt ggttcaggca
     7441 cattggagta taaggggcag aaatatacgc ttcaagtagg agatatggtg tttattgact
     7501 gtttgaagcc gtatgtccat gagtcatcag aagatgattt gtggactttg cggtggtgcc
     7561 attttaatgg cattggtatg acggaagtct acaaaaaata ccttgaacga ggtggtcaac
     7621 cggtctttaa aacatcaaaa acagataagt atgctgactt gctgaggagc cttcatatgg
     7681 tggcagaggg acaggagtat gtccgggata tgaaaatcaa tgttctactc agtgaactgc
     7741 tgatgcattt gatggaagat tcttggaatc ctgacaaagt gccgaatttc tctacaaaaa
     7801 aaatggaatt gcagaacata aaacaatatc tggatgacca ttacacggag aagattacgc
     7861 tggatcagtt gagtgcagag ttttttatca ataaacagta cttagctaag atttttcgtg
     7921 aaacttacgg tacaactgtt gtcggttatc tgaatcagaa acgaatcaca caggccaagc
     7981 acttgctacg ctttacaaat ctaagtatgg aagagattgc aacaaaaatt ggcataacag
     8041 atgcaaacta tttttccaga acttttttca aggtggaagg tgttcgtccc agcgaattcc
     8101 gcaagatgtg gtaataaaaa gccggcggtg ttgagccgcc ggtcagtacc tgtcgttaac
     8161 ccgcattcaa agctttaatc tcagctttga tttcagcacg aacacgatag taatcttcag
     8221 tttcgatttc ggtatccagc cgagttgctg tctttccgca gtcaggacaa cgctctggct
     8281 gttcgtcggt agggaagctg tagtggcagg aatcacaggt gtaaatgtga tcgtcacgta
     8341 taatcatcac ctaccaatcc agtgcttaga tttttcatgc aattactatt atatcgaatt
     8401 ggtgattcca caaaaatccg cttcttgata aatgtacgat tcagagtgat atatagtact
     8461 gtactgccaa agggaatacc acaacggcag aaatggatga ttcacatgga agaaattatg
     8521 cagatgattt tagggaacgc aaaattccag ctcggacagg tggtttcgac ccctgctgta
     8581 ttggaaactt tcaaccccct gcagaccgca aacggcattc ccgaagcctt gagcaacctt
     8641 tggtttagac aggaaattag tattgcggag atcgaggacg cagttctgga tgccaagaac
     8701 ggcaaggagc tagcaaacag cctcaaccgc atcaagcttt tccgcaagtt caccctcgac
     8761 cgagagctgg acggcatggt cagactgaag ggcgcggacg tctggaagaa catcagctac
     8821 ctcaaggtct acaccgccac gccgcctaag aaaccgaccc ccccaaggtt ccagtcgaac
     8881 ggctggagcc ttttcttctc tcataaatcg gcataatgta tacaaataca gctccaaatg
     8941 attgtgtagt atatgcctgt ggattgactt gatatagtcg agagactatg gcaatataca
     9001 cacaaccaaa ggggaaaatc ccaaaaacaa ggaggaaacg ctaatggcaa tgagcgaaaa
     9061 ggcaaagaaa tatttgaagg agatcaaggg tgcaaagacc atcccggagc tgaaggatgt
     9121 ggagatcgca atcaagcgag acggtatcct tgcttgggca gagttcacca agttgaacga
     9181 ggcggtggaa gagaagaagg tcgcccttcg caagaaaaag caggagacca gccttcagga
     9241 gatccttttc tgggcctaca aaaaggaatc agacaagctg ctgaagatga tggacgaggg
     9301 agcagacaag gatgccatcc agatgcaggt ccagcgttac gacagcatcg gccagatcat
     9361 tgcagaggca gacctcgaag atgagtatga ggtttgagaa tacagcctga accactaaga
     9421 accaaatacc gaaagggact tctgaaacag acagaggtcc ctttcttttg ctcactttgg
     9481 cggtcaatta tgaaccaaaa accagtcgaa tgattgtgta cattatggct ttcaaacgac
     9541 ttgataataa tcacttttag agtgatatat gtactacaaa aaacaaaaga ctggagaaaa
     9601 caacagatga gcgaatacag aatcatttat atcagcgacg aggacggcgg cgaggtggaa
     9661 ggatgtggct acacggctac gatggttatg atgcggtcag aaacttcttg gaatttgcct
     9721 gcggcatctt gaaaatcctg cgggtgcgat tcatcagaag agtggactga aatacaagtt
     9781 agcagataga gaagagagtc aggtttcgac ttggctctgt tctctgtcta ctggcagaaa
     9841 tacattgaag ggaggtgacg actgtggcaa ccagaggaag aaaatcaacc ccgacagcca
     9901 tgaagaagct ggagggaaat ccgggcaatc gcccactcaa tgcaaaagag tcaacgccgg
     9961 aaaagaaggc accgacctgt ccgaagtggc tggaaccaga ggcaaagaag gaatggaagc
    10021 gtctggcaaa gcagatggag gccatcggaa tcttgacaga tgtggatatg gctgcctttg
    10081 ccggttagtg ccaagcctac gcccgctgga aggaagccga ggaattcatt acccagcacg
    10141 gtatcatcgt gaaaacccta tccggctatt ggcagcaggt accacaggtg tttattgccc
    10201 agacctatct gaaaattatg aaccgatttt ccgagcagtt cggtctgacg ccttcgtcca
    10261 gaagccgtat cattgccggt gatggcggtg gtgtcggagc agaagatgcg atggaggctc
    10321 tgttgggagg tggtagctga tggcagaaac cagaccaaag ggctatccca aattaaagga
    10381 atacacacca aaccgattca tgctttcgga atgtcactac gacaaagcca gagcggatcg
    10441 ggcggtgaac tttatcggac agctccgaca caccaaaggc aaatgggcag gcaaccgctt
    10501 ctggctcttg ccttggcagg aacagatcat ctagtgtaat ctcgcttaac tgtattaacg
    10561 gacgtagcga ctttattaag agtgttcatg gtaggagcat tagttgcaac aatgattgat
    10621 attaaatttt tatggtacca acgattggcg atgtgacatt gaatatgcaa aactatatcg
    10681 atatgatatt atctaaaatc ttactcgctg ggatcgtcga ggtgtttatt ggttgttagg
    10741 aaagaaaaat atgacccaac tcgagcgatt tttatcgtat tacttgtgtg tggctttatc
    10801 tgcattgtgt gtgatttcga aataagggag agccaaaaat gagtagaaaa ttgatattga
    10861 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac aaaaatgatt atgggacctc
    10921 aagagaatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacagacaaa
    10981 agctcttact gtcatagatt cgttaaatga ttttgtggta ctatgcgatc ttttaggcgg
    11041 tacacctact aatatcattt ctaaactgat aatggaagga caacagatta aactttatgt
    11101 cggattaatc ttcctatggt cattgaattt atcaactcgc aagtgattgg ttcagagccc
    11161 gagtttgttc gtgcaggatg tgaaagcatt gtatctgtca actaattaat tagtggaata
    11221 gtagaagatg aagag
//
LOCUS       DASHWH010000080        11051 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.083, whole genome shotgun sequence.
ACCESSION   DASHWH010000080 DASHWH010000000
VERSION     DASHWH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.083"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            152..1141
                     /locus_tag="U2R41_002338"
     CDS             152..1141
                     /locus_tag="U2R41_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889713.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1123..1584
                     /locus_tag="U2R41_002339"
     CDS             1123..1584
                     /locus_tag="U2R41_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM8889714.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1634..2098
                     /locus_tag="U2R41_002340"
     CDS             1634..2098
                     /locus_tag="U2R41_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889715.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2112..2861
                     /locus_tag="U2R41_002341"
     CDS             2112..2861
                     /locus_tag="U2R41_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8889716.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2861..3691
                     /locus_tag="U2R41_002342"
     CDS             2861..3691
                     /locus_tag="U2R41_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8889717.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3691..4338
                     /locus_tag="U2R41_002343"
     CDS             3691..4338
                     /locus_tag="U2R41_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8889718.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4436..5212)
                     /locus_tag="U2R41_002344"
     CDS             complement(4436..5212)
                     /locus_tag="U2R41_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8889719.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5294..5437
                     /locus_tag="U2R41_002345"
                     /pseudo
     CDS             5294..5437
                     /locus_tag="U2R41_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5554..6732)
                     /locus_tag="U2R41_002346"
                     /pseudo
     CDS             complement(5554..6732)
                     /locus_tag="U2R41_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            6762..7172
                     /locus_tag="U2R41_002347"
     CDS             6762..7172
                     /locus_tag="U2R41_002347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889720.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            7253..7873
                     /locus_tag="U2R41_002348"
     CDS             7253..7873
                     /locus_tag="U2R41_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889721.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(8203..9429)
                     /locus_tag="U2R41_002349"
     CDS             complement(8203..9429)
                     /locus_tag="U2R41_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8889722.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(9501..9659)
                     /locus_tag="U2R41_002350"
     CDS             complement(9501..9659)
                     /locus_tag="U2R41_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889723.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            10347..>11051
                     /locus_tag="U2R41_002351"
     CDS             10347..>11051
                     /locus_tag="U2R41_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8889724.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         3693 a         1591 c         1928 g         3839 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaatttttt tgaaaaatag tattgcaaaa taaagcaatt tcatagataa taggtatcgt
      121 aaagttatat aactttataa tacctattag agtgagggat aaagaaatga gacaactaca
      181 caaagaacag attgaaacag caatggcagc gttaaaggaa aagacaattg atcatgtata
      241 ttttacagca tgtgggggtt cacaagccgt tttaatggct ggtcaatatc tatttgataa
      301 agaattaact gtaccttgtc atgtttatac tgctaacgaa tttaactatg ctataccaaa
      361 aggattatct gaaaattctt tagttatcac ttgttcccat tctggcacaa caccagaaac
      421 tgtagaagca acaaaaaaag caagagaatt tggagcaatg actatttgtt tatctaatga
      481 agaaggatct cctctatggg aagcagctga atatcctgtt cactatgatt ggggtaaaga
      541 tgttgatgct agtgatttaa ataaaggaat tctttatggt ttattattta atattttact
      601 tgttttagat ggctctaaaa aatgggaaac ttgcttgaat gaatttgata agttaaatga
      661 tcttgttatt gctgaaaaga gcaaatttga aataactgct aaagaatggg gtaaaaacca
      721 aaaacgagat aaaattattt acacaattgg tagcggtatt aattacggag aaatttactc
      781 cacagcagct tgctggttca tggaaatgca atggattaat tctaatgcta ttcattctgg
      841 ggaatttttc catggtcctt ttgaaatcac tgattatgat gtaccattca tcttagtgaa
      901 aagtattgga gatacaagat ttttagatca acgggtagaa gacttcacta aaaaattcag
      961 taataaatta atggttttag accaagctga ttttgactta gaatctatag atagtgtagc
     1021 gaaagaatat attgcagctg tacttagtgg cgttgttatt cgccaaatgg tagaagcgat
     1081 tgcttttgaa agaggccatt ccttagatgt tagacgctat atgtggcaaa tgaaatatta
     1141 attaatagga ggttgcaaaa gatggcaaat caattatttc acattgtgat tacaacacat
     1201 tctacccttt gtgagggtta tttaaaggca acaaaattaa ttttaatgaa tgacgccaca
     1261 ggtattagtg ctgttccatt taaagaaaat actagtccag atgattttga ggagagtatg
     1321 aaagaaataa ttagtcagca tcatttgcaa cctctaatta ttttaacgga tttaattgga
     1381 ggaacaccta ataatgtttc tattaaacag ttgaacaaca atagaattca aattattgct
     1441 gggattaatt tacctctttt actggagtta ttaatggccc agtcagctgg taaatctata
     1501 gaagaaatag attttaagga attactgaat aactctcaaa aaagtatggt tcatattaat
     1561 tcgcttttaa aagatgtcag ttaaggtggc aatttctatt ctataaattt taaaaattga
     1621 aggaggaaat agaatgatta aattagtgcg tgtagaccac agattacttc atggacaagt
     1681 aatattttct tggactaaac agtatgatat taaccatatt attgtggctg ataacaaagt
     1741 tccaggagat ccaatgtctg tgatggcttt gacaattgct aaacctgctg attgcaaatt
     1801 agacattgtt aaaattagtg atgttaaagg gattatggaa acatccccaa atgataatta
     1861 catgatttta attaaaggac ctaaggaagc ttttgaatta gttcaaaatt taccagaaat
     1921 taaagaaatt aattttggcg gtgtgtctaa gaaaagcgag tccaaacaat atggaaaggc
     1981 agtcttttta actcctgaag aattagaagc cacaaaaaaa attcttgatc aagaagtgaa
     2041 aatatttgtg caacaagtcc catcttcaag tattgaaaat gtagatttta gtaagtaagg
     2101 ggggaaaata aatgttatta aaagccattt tattaggttt agttggtgtg gtagctgtta
     2161 ttgactccag gttaattggt cgacaaaata ttggtagacc actaattttg agtacacttg
     2221 ccggacttgt gctaggtgac ttgcatacag ggattgttct tggggcatct ttagaattaa
     2281 tttctatggg attcgtctca attggggcgg ctggacctcc taatatgcaa cttggtagta
     2341 ttattgccac tgcttttgca attttaacta atagtagtac agaaacagcc ttaacaatag
     2401 ctatcccagt ggctgtagct ggtgaattta tgagtattat catgcgcatg ataattgctc
     2461 aatttggaca tgctgcagat aaagccattg aaaataataa atttagaaaa gctcaaatta
     2521 ttcatattta ttggtcattt atttttaatg cttttgttta ctttattcca atctttttaa
     2581 cagtatattt tggtgctgac gttgtggcag atttagtaga taaaattcca gaaatgatta
     2641 cagacgcctt gactgtctct ggaaatatgc tttccgcatt agggtttgca atgctattaa
     2701 gcacaatgtt gagtaaaaaa ttatatcctt actttatttt tggattcttt attgttgcct
     2761 attcaggtct ttcactaatt ggcgtgacaa tttttgcagc tctgattgcc tttgtaatgg
     2821 atcaagttaa atatggaaaa agagaagagg cgcatggtta atggaaaata gtacagaaaa
     2881 aaaacgttta atcacaaaaa aagatcttca taagatgtgt cttcgttcat ggacaatcat
     2941 gtgttcgtgg aattatgaaa gacaaatgca tatgggattt atgtatggga tggcgccaat
     3001 tttaaataaa gtttataaaa atgatgaaga gaaaaaaaga gaagcttata ctcgtcatat
     3061 ggcatttttt aactgtacgc ctcaaatgac tccatttatt atgggacttt gtgcttctat
     3121 ggaagaacaa aatgctcaag cagaagaagg aaactttaat actgaatcta tctccatgat
     3181 taaaaccagt ttaatgggtc cttttgctgg aattggtgat tcatttttcc aaggtacttt
     3241 aagaattatt acatttggga ttggattgtc ttttgctaag caaggtagta ttttaggacc
     3301 gattttagct gttggccttt ttgctatacc ttctatttta gctgtttttt acggcggtaa
     3361 attaggatat gagtctggca ataaatacct tactaaattg tatcaagaag gtcttatgga
     3421 tcgagtaatg caatttgctt ctatcattgg tttggctgta gtaggtggaa tggtggctag
     3481 catggtgcca attgtcaccc ctttaaaatt cactacaggg ggaacagaac ttgttttaca
     3541 agacttactt gattccatta ttcctggatt attaccactt gtatgtactt tgggaatata
     3601 taaattgatt aagaaaaatg ttagtacaaa tatgctctta ttaggttgta ttttggcagg
     3661 gtttgtttta agtataatag gtatcttata atgattaagg ctctaatttt tgatatggac
     3721 ggagttttaa ttgatagcga gattatgtat caaaagtggg tatttaattt tttaaaacaa
     3781 gaaaatttaa gctatccttt gtattcttat caaaaaaaga ttggaactac agcaagtatt
     3841 tttgatgata tagaaaagta caatgaggga tgtaacagtt tagaattgaa gggaaaattt
     3901 actaaatatt ggaaatcgca aaagattaat tacttggata tttttcctaa tatactaatt
     3961 gaacagcttg aatggttaaa aaagaaaaaa ataaaaatgg caattgcttc ttccagtcca
     4021 atggatacta taaatgaagt tgttaccact tgtaaaatag agtcatattt tgattgttta
     4081 attagcggaa gagatttgcc agaaagtaaa cctaatccta ctatattcct taaagcttca
     4141 gaacagctca gaattccagt tgaagaatgt ctggtgatag aagattctta taatggtata
     4201 aaagctggta aaagagcaaa tatgaaggtt cttgcaatta aagataaacg tttttctcaa
     4261 gatgtaagtc tagcagatga tgttatttac gatattaaat atctgcgaca agaagtacag
     4321 gtgaaatttg gaatataaat aagttttttg atgtatttag agaatattag taaaaataaa
     4381 aatcatgccc aaatctaatc aaaattgatt gctttgggta tgatttcttt taatatcaat
     4441 ctattacttt aggatctgaa tcatttttat gcaccgtatc taaagtgata ttttctgtga
     4501 cttcaataac ataactaatt ctatcacaac gaactgtgga ttgagaataa tgaaatggtt
     4561 gattattttt atctttgcca accttttcta aaataaatag cggatctcct actatacatt
     4621 gcaataagtc ggaaagctca ggtgttgcag ataaaccgtt tatttccata atagagctcg
     4681 tagctttgac atcatagtca tcttctagaa gtttatataa tgaagtatat tggctacctt
     4741 tttcaaataa agttggaaat ttttcttctg atgcgaaaaa attatcaatt cccatagggg
     4801 caccgtcttc ccacattaaa cgtttaatat tgtaaactct atcagttaca ttgatttgta
     4861 atttattagc taaaaactca tcagccttta taattttaga atgtaaaaca gtatatctaa
     4921 ttttatgtcc tcgggtagaa agagaatcgc taaatccatg aactccagct aattttaaac
     4981 tttctttttt ttgagttaca aaggtccctt tcccccttaa tcgttcaact gttccacttt
     5041 ctgttaaaag tttaaaagct tttcgaatgg taatcttact tactccataa attttacata
     5101 attcttcttc tgtaggtaat tgctctcctg gaagatattt tttttcacga atatcgttac
     5161 gtatagaagc ttctacttgt agatataatg gtctattatc ttgaaaattc atagtacact
     5221 ccctaaaaag ttatacttca ttatatcaca atttattaaa ataaaagatt aagatataaa
     5281 tataaatgaa ctagtctttg actaaccaat gcccgctttt gaaagaaaac aaattcattc
     5341 tgttttaagt aaaaatcctt atgttaccac tcattctgaa ggggatgagc cttatcgcta
     5401 cttggttgtg gaaccaagtg ataagacaat tttataaatt caatattaat taaaataaaa
     5461 acaaacaaag tagattattt tactttgttt gtttttttat tgggagcgtc aataattttg
     5521 tgtaaataac tcgtccttct gcaaaataat gggttactca gtaaacattg aagctaatgt
     5581 atccgtcact tgttggaagc ctttatggct tctatttaag aatttttgat tgtatgtatc
     5641 aaagatggat actaggaaac gctctagtga ttcttcattt tgaaactgct cttttctgcg
     5701 gctgtatttc ttgatttgtt tcttgaaaga ttcgattaaa ttagttgagt aaatgcttcg
     5761 gcggatacca ggcggaaact cataaaaagt caataaatct tgatttttta tcagtgactg
     5821 cgtcacttta ggataagttt tttgctactt ctcaatcata ctccctaaaa aggtatttgc
     5881 ctcttccttt gagctagctt gataaacagc cttaaaatca tcatagattt cttttcgatc
     5941 tttgacacgt actttatgag caatattacg ggaaacgtga acacagcaat gttggtattt
     6001 agctttagga taaatctggt gaatcgtatc tttcatgcct tttaagccat cggtaataaa
     6061 cagtaagaca tcatgaaccc ctctggagta aatgtcctgt aacaactcat tccaaacgta
     6121 ggtcgattca gtcggagcaa tcgcataact tagtacttct ttagtgccgt cttctcgtat
     6181 accaatggca atatagattg cttctttgga gacggtttgc cgttttaaag gaatataagt
     6241 agcatccatg aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac
     6301 ttcttcagtc agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata
     6361 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
     6421 gacggtctcc tctaaggtat cattcgtccg tctataagca ggaacagtct gttgcttgaa
     6481 ctcgccgttg cggtcgcgcg gaatctggag atgaagttcc ccgtactcgg tcttgaccgt
     6541 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc
     6601 gtaatcgagg aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg
     6661 gacacggaat aattcattca aatcgccttt gttcgctaga gtctttagaa tttctgtagt
     6721 aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca
     6781 gaagggactt ccttttttct atttacacaa aatattttac actctcgtca aatataaaaa
     6841 ctagtacgaa attaatttgt aaattattta agcttgataa aataagcaat atcgatgatg
     6901 taaagtccaa gatattaaca agaaaagtaa gcgaaccagt aaagctaacg gcacaagata
     6961 aaaaaaatgg aattgttctt tatggtgatg atgcaaagta taactataat tgggatacct
     7021 cctcagaagc tgaggtatta agtattccta ctaccgatac ttcaggggaa aaagtcacct
     7081 ttaaaataaa ttttataacg gcaatttcta agctggagta tgggaagcaa aaaaagagag
     7141 ataacaatga tcaatatcta cctaaaaaat aacgaattaa tgatgcatat gttaaagttg
     7201 tttttttgaa ttaaacaact caatttattc agttatatgt tcatcgcagc aagtgaacat
     7261 taatcgagtt ataaaattaa taggagctaa aaatataaat aaagttcgat cagaatttat
     7321 ttttccagaa gattttttta actggttttt ttatcaattc agtgaatgca agcgactctt
     7381 aggtgagaat tattacttaa aaggaatcgg cggatttatg gggaatgtgg ctgatgaaca
     7441 aaatattata aaaggaatat ctgaacaaac atcagagctt acagtaacta aagcatttat
     7501 aagtaatggt gaaagtttga aaaatgtaac tgcgcgaatt agaaaagaaa atgaagtaga
     7561 aattgttttt ggaattgacc atgaatgtaa ttcagtgata tatgtaacac aatcagcaaa
     7621 tttaattctt tttgagaatg gttgcaagga cgattttatg gtggtctatt tgtacgatat
     7681 actaattcca aaacttcgtt ccttatatat gtcttctgaa agtatttttg tgagtgaaca
     7741 aaagggatct ttctcgaaaa aaataggtat tgaagtaatt gaatcgatta tgtgtcataa
     7801 tcatattacg ctagaagaat tagaaatttt gatagggcaa aaaaataata acacgaaaat
     7861 acaaatttct tagaaatcat tttttattga gatatctaga taaatgattt attcttcaat
     7921 caaccaactc gccaagctga ggtaatgttt tgaggtaatg ataacataca actttttata
     7981 ccctagtaaa actctataaa caaagactta ttaatttcgc atgaaataca ttcctgtaaa
     8041 atgcaataaa atagagtttt gtttaaccag gtctcaaaaa ggctcgtaaa gcatctcaat
     8101 tctctaaacg ttaatattat ttatatatca atgtttcaag gcacttttca attgtttgaa
     8161 aagtgtcttt ttcttgttta agaacacatt taagaacaca tactaatcac atttattcaa
     8221 tcctctctga aaggcttcaa aaacagtttt atctactaca tctggtgtat ttacgtatat
     8281 tctcgttgta gaaatatctg agtgtgttaa agcactagat atttgattca tatttgctcc
     8341 gccttgtcta gctatagttg cataggtatg acgaagctta tgtggagata aatgagccaa
     8401 ttcaggatgt cgtcttttaa ttgaattgat tcgatagttt aggtaatcag cgtgaacagg
     8461 gcaatttact tctccggatg gtttagtata ggtaaagaga tactgatctg gtgtttgatt
     8521 aatttttaat ctcaacaatt gctcagcttg cactgatttc catttgctta gtaatgccat
     8581 tacaatttcg ggtacttgaa ttacggtatc ctttcgacct tttgtaaatt tctttttttg
     8641 atatttatcc aaagcatgtt taaggcgaac cgtttgtttc tcaaagtcga tatatttcca
     8701 ttgcaaagca tacgtctcac tttttctatc accaagatat agggtaagca tgaaaagagt
     8761 atagtcatga agagtaagca agtgattatt caagtcagtg tttactgctt ctatccaagc
     8821 aataagctct tctgacgata atgcttgatt accttcccgt atccttcttt cttctaaagc
     8881 aattttttta ggtgcagtga tacattgaat aattttggta gttcggttaa attcaatata
     8941 atttagtatt tctgcaatat caaacataga acggacatat cctttgataa tttttatatt
     9001 tgcgtattct ttcgattttt ttgttaattc atctgatatt atctcagtat ttgaatttag
     9061 atagttcatt gcatactcac caaacattgg aaggatgtgc aatcggaata tatctttagt
     9121 attactaatt gttacgtaac ttggagctct ttttgatcta gtagtctgac ctagttcata
     9181 tctaggaagc cattttgatt catagaattc cttaaatttt atttttcctt ttaattctag
     9241 tgaatttgca ttctcctctc ttaaaactgt ttcaattttc cttttcattg cattttcggc
     9301 tttaatagcc aattgcctag ttggaaatac tttttgatac ttttctgatg taattccagt
     9361 gatttctctg aatttttttg gatacctcag attagtttta tatttcccat ttgaaagttt
     9421 tgtaggcaaa attcctcact cccttaaaaa gtaaggcagt atatttcgct attcttagta
     9481 tatgcggcga gaagaaattt tcaaattgtt ttaaatgtat tcataaaatc tttgatatcc
     9541 tctatgtcaa agcgaactat accgtcaatt ctgattactg gaaaattata tatacgaatc
     9601 cacttatcta aagtattatt atcaattcct agaaactcac aggtttgttt tttattcaag
     9661 tagactctca attttataaa atcatctcct aaaaaagatt agaatttttg aattgctttg
     9721 ggtaagcaca taggaatttc acctctcccc agtttacggc gagccttacg gaagtatcat
     9781 tatttgctgc tattgtcatg gcattttgtt ttcccagaac aaaaagaaat ctctaagtat
     9841 ttgtcgctaa tcaggtcatg gcgtacttga gtttctagct cgaatagcag ggatcgttat
     9901 tcaaaaattg tatttaattg tcaatgatca aaaataggtt ttttcctatt cactattaat
     9961 aacgataaac atgtataaaa agacacacta cgaaaaaagt taaatggagt ttactgattt
    10021 tcatatttgt atatggaaca gacatagtct tgaataattt ttataaatgt tttgatgctt
    10081 atcaacttat tcaacccgct atccacttta agtataattt ttccatacgc caataaagta
    10141 gttttaggag catgttctca tttgagaacg ctcctttttt atatattttg aaagctactt
    10201 actatacgcg tagtttcaaa tagttttgtc attctaatct tcagcaacag aaaaaaatct
    10261 aaattataaa atttagggtt gactaaaata taatttttgt ataccatatg atcgtgataa
    10321 tcattatcat ttagaaggag aaagttatgt tagaagtgaa aaagcttaat gtttgttata
    10381 acgatttttt agcattgaac gagatttctt taaaaataca agaaggtgct ttaacaggta
    10441 ttattggtcc aaatggtgct ggaaaatcta cattgcttaa agctatgtta aatattatcc
    10501 ctcatcaggg agaggtctcc attaataatg aagatatcaa taaaaaactg gtgaagattg
    10561 catatgtaga gcaaaaagct gatattgatt ttacttttcc aataaaagta aaagaatgtg
    10621 tatctatggg aacatatgcc ggaatgaaag tttttcaaag aattaaaaat gcagaatggc
    10681 aaagagtttc aaaggctttg gaaaaggtcg atatgggaaa atattctaat catcaaatag
    10741 gagaattatc tggaggacaa tttcaacgtg tacttttagc aagatgtctt gctcaaaatg
    10801 cggatttcat atttttagat gaaccatttg tagggataga cttagttagt gaacgaataa
    10861 ttatggatac gttaaaagaa ttgaaacatc agggaaaaac agtgctaatt gtgcatcatg
    10921 atttaagcaa ggtaaagaag tattttgata atattattct tttgaatcga aatctaattg
    10981 ctcacggaag tgtagaatct gtatttaatg aagagaattt aaaaaaagcc tatggagata
    11041 ctatatttat t
//
LOCUS       DASHWH010000081        10978 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.084, whole genome shotgun sequence.
ACCESSION   DASHWH010000081 DASHWH010000000
VERSION     DASHWH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.084"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(57..1433)
                     /locus_tag="U2R41_002352"
     CDS             complement(57..1433)
                     /locus_tag="U2R41_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM8889725.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1561..2679)
                     /locus_tag="U2R41_002353"
     CDS             complement(1561..2679)
                     /locus_tag="U2R41_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8889726.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2821..3255
                     /locus_tag="U2R41_002354"
     CDS             2821..3255
                     /locus_tag="U2R41_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889727.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3333..3866)
                     /locus_tag="U2R41_002355"
     CDS             complement(3333..3866)
                     /locus_tag="U2R41_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEM8889728.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3960..5138)
                     /gene="mutY"
                     /locus_tag="U2R41_002356"
     CDS             complement(3960..5138)
                     /gene="mutY"
                     /locus_tag="U2R41_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEM8889729.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5131..5928)
                     /gene="recX"
                     /locus_tag="U2R41_002357"
     CDS             complement(5131..5928)
                     /gene="recX"
                     /locus_tag="U2R41_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEM8889730.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6035..7417
                     /gene="rlmD"
                     /locus_tag="U2R41_002358"
     CDS             6035..7417
                     /gene="rlmD"
                     /locus_tag="U2R41_002358"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM8889731.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7591..8169)
                     /locus_tag="U2R41_002359"
     CDS             complement(7591..8169)
                     /locus_tag="U2R41_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEM8889732.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8491..8961
                     /locus_tag="U2R41_002360"
     CDS             8491..8961
                     /locus_tag="U2R41_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEM8889733.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9069..10295)
                     /locus_tag="U2R41_002361"
     CDS             complement(9069..10295)
                     /locus_tag="U2R41_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8889734.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3277 a         2190 c         1843 g         3668 t
ORIGIN      
        1 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
       61 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
      121 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
      181 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
      241 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
      301 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
      361 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
      421 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
      481 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
      541 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
      601 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
      661 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
      721 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
      781 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
      841 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
      901 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
      961 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
     1021 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
     1081 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
     1141 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
     1201 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
     1261 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
     1321 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
     1381 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
     1441 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
     1501 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
     1561 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
     1621 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
     1681 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
     1741 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
     1801 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
     1861 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
     1921 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
     1981 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
     2041 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
     2101 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
     2161 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
     2221 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
     2281 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
     2341 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
     2401 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
     2461 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
     2521 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
     2581 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
     2641 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
     2701 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
     2761 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
     2821 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
     2881 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
     2941 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
     3001 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
     3061 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
     3121 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
     3181 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
     3241 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
     3301 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
     3361 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
     3421 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
     3481 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
     3541 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
     3601 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
     3661 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
     3721 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
     3781 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
     3841 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
     3901 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
     3961 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
     4021 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
     4081 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
     4141 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
     4201 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
     4261 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
     4321 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
     4381 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
     4441 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
     4501 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
     4561 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
     4621 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
     4681 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
     4741 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
     4801 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
     4861 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
     4921 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
     4981 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
     5041 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
     5101 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
     5161 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
     5221 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
     5281 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
     5341 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
     5401 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
     5461 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
     5521 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
     5581 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
     5641 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
     5701 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
     5761 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
     5821 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
     5881 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
     5941 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
     6001 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
     6061 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
     6121 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
     6181 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
     6241 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
     6301 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
     6361 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
     6421 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
     6481 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
     6541 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
     6601 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
     6661 cggcgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
     6721 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
     6781 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
     6841 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
     6901 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
     6961 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
     7021 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
     7081 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
     7141 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
     7201 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
     7261 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
     7321 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
     7381 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
     7441 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
     7501 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
     7561 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
     7621 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
     7681 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
     7741 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
     7801 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
     7861 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
     7921 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
     7981 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
     8041 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
     8101 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
     8161 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
     8221 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
     8281 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
     8341 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
     8401 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
     8461 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
     8521 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
     8581 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
     8641 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
     8701 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
     8761 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
     8821 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
     8881 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
     8941 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
     9001 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
     9061 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
     9121 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
     9181 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
     9241 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
     9301 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
     9361 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
     9421 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
     9481 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
     9541 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
     9601 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
     9661 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
     9721 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
     9781 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
     9841 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
     9901 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
     9961 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    10021 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    10081 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    10141 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    10201 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    10261 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    10321 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    10381 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    10441 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    10501 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    10561 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    10621 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    10681 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    10741 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    10801 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    10861 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    10921 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DASHWH010000082        10714 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.085, whole genome shotgun sequence.
ACCESSION   DASHWH010000082 DASHWH010000000
VERSION     DASHWH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.085"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1235)
                     /locus_tag="U2R41_002362"
     CDS             complement(<1..1235)
                     /locus_tag="U2R41_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM8889735.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="U2R41_002363"
     CDS             complement(1228..2820)
                     /locus_tag="U2R41_002363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM8889736.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="U2R41_002364"
     CDS             complement(3330..4055)
                     /locus_tag="U2R41_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889737.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="U2R41_002365"
     CDS             complement(4209..4535)
                     /locus_tag="U2R41_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889738.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="U2R41_002366"
     CDS             complement(4572..4937)
                     /locus_tag="U2R41_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEM8889739.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="U2R41_002367"
     CDS             complement(4974..5189)
                     /locus_tag="U2R41_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889740.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="U2R41_002368"
     CDS             5318..5653
                     /locus_tag="U2R41_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889741.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="U2R41_002369"
     CDS             complement(5729..7726)
                     /locus_tag="U2R41_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889742.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7824..8099)
                     /locus_tag="U2R41_002370"
     CDS             complement(7824..8099)
                     /locus_tag="U2R41_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889743.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8331..9065
                     /locus_tag="U2R41_002371"
     CDS             8331..9065
                     /locus_tag="U2R41_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889744.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9104..10246
                     /locus_tag="U2R41_002372"
     CDS             9104..10246
                     /locus_tag="U2R41_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8889745.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3451 a         2003 c         1583 g         3677 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagaac ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgacatagat tatgaggaag agtttttggc tctctatcgc tctcttagta ctgaagatgc
     5581 aattcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatat aattgggtct cttctattcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaatagga actaattcaa
     5821 ctcctgataa tatcaattct tttattactt catctatccc tgcccctttc gctagatata
     5881 ctaaaaaact actgcttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttc tattaaagca tttttcacgc
     6001 tgttatccct gacatttcgt aggcgttcac aaacagtgtt aataagttct ttttctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gtttaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtgtcctcct
     7741 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     7801 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     7861 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     7921 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     7981 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     8041 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     8101 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     8161 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     8221 catcgacata cacataatac ctcgataaaa aaggggtgtc aatagttttg taaaatttat
     8281 ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
     8341 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
     8401 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
     8461 ttgacaaaaa gactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
     8521 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
     8581 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
     8641 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
     8701 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
     8761 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
     8821 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
     8881 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
     8941 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
     9001 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
     9061 tctagtggta agtactcctt gtaaactaag aggagatgat ttcatggcaa aaatatcaaa
     9121 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
     9181 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
     9241 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
     9301 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
     9361 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
     9421 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
     9481 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
     9541 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
     9601 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
     9661 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
     9721 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
     9781 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
     9841 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
     9901 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
     9961 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    10021 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    10081 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    10141 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    10201 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    10261 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    10321 ttctagctcc tataacaggt ttacccaaca ctgccttcca atgactcttt tgggaatcag
    10381 aggaaagaat caggctttat agagatattt catctaacaa attactttag actgacagta
    10441 tatatcagca ggtactgtca tcgggaacgt agccaatatc gtatccaaaa cagcaggtag
    10501 agaagctatt tgaggattca agttattcta tttcaaccat gaagttaagt tcattaaaaa
    10561 taaagatagt tggtatttca ataatttttg tatagagtta atcactttat tgatgctaat
    10621 tcatcttgat tggtattata atcgtattca agttgcgtgg cgtgcttttg ctagaaaata
    10681 tataactgtt ctttataaag aaatataagg atta
//
LOCUS       DASHWH010000083        10433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.086, whole genome shotgun sequence.
ACCESSION   DASHWH010000083 DASHWH010000000
VERSION     DASHWH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.086"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            87..305
                     /locus_tag="U2R41_002373"
     CDS             87..305
                     /locus_tag="U2R41_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889746.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="U2R41_002374"
     CDS             complement(594..1106)
                     /locus_tag="U2R41_002374"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEM8889747.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="U2R41_002375"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="U2R41_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEM8889748.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..4002)
                     /locus_tag="U2R41_002376"
     CDS             complement(3559..4002)
                     /locus_tag="U2R41_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEM8889749.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKNEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="U2R41_002377"
     CDS             complement(4003..4791)
                     /locus_tag="U2R41_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8889750.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="U2R41_002378"
     CDS             complement(4788..5729)
                     /locus_tag="U2R41_002378"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEM8889751.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="U2R41_002379"
     CDS             complement(5906..6493)
                     /locus_tag="U2R41_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEM8889752.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="U2R41_002380"
     CDS             complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="U2R41_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEM8889753.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="U2R41_002381"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="U2R41_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM8889754.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="U2R41_002382"
     CDS             complement(9410..10345)
                     /locus_tag="U2R41_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8889755.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2887 a         2356 c         1809 g         3381 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcattt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DASHWH010000084        10240 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.087, whole genome shotgun sequence.
ACCESSION   DASHWH010000084 DASHWH010000000
VERSION     DASHWH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.087"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1218
                     /locus_tag="U2R41_002383"
     CDS             <1..1218
                     /locus_tag="U2R41_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM8889756.1"
                     /translation="NSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSM
                     FVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPY
                     EIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLF
                     SLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILI
                     ILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNY
                     PSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIA
                     INLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYP
                     TQLLDKIEFDYNI"
     gene            1418..1732
                     /locus_tag="U2R41_002384"
     CDS             1418..1732
                     /locus_tag="U2R41_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8889757.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1753..2130
                     /locus_tag="U2R41_002385"
     CDS             1753..2130
                     /locus_tag="U2R41_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8889758.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            2140..2913
                     /locus_tag="U2R41_002386"
     CDS             2140..2913
                     /locus_tag="U2R41_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889759.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2935..4338
                     /locus_tag="U2R41_002387"
     CDS             2935..4338
                     /locus_tag="U2R41_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8889760.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            4520..5704
                     /locus_tag="U2R41_002388"
     CDS             4520..5704
                     /locus_tag="U2R41_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8889761.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5701..5991
                     /locus_tag="U2R41_002389"
     CDS             5701..5991
                     /locus_tag="U2R41_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889762.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5988..6209
                     /locus_tag="U2R41_002390"
     CDS             5988..6209
                     /locus_tag="U2R41_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889763.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            6251..7030
                     /locus_tag="U2R41_002391"
     CDS             6251..7030
                     /locus_tag="U2R41_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEM8889764.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            7097..7591
                     /locus_tag="U2R41_002392"
     CDS             7097..7591
                     /locus_tag="U2R41_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8889765.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            7647..8285
                     /locus_tag="U2R41_002393"
     CDS             7647..8285
                     /locus_tag="U2R41_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889766.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            8357..8752
                     /locus_tag="U2R41_002394"
     CDS             8357..8752
                     /locus_tag="U2R41_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8889767.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8736..>10240
                     /locus_tag="U2R41_002395"
     CDS             8736..>10240
                     /locus_tag="U2R41_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8889768.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT         3560 a         1627 c         1974 g         3079 t
ORIGIN      
        1 aacagtaacg ttgcacgtaa tattgctagt aatataaggg cgatttatgt ggatgaattt
       61 caagatacta gagaattaca atacaatgta atagctaaat tacttcaaaa caaccctcaa
      121 attcagtcaa tgtttgttgg agatattgac caagcaattt atggatcatt agatggtata
      181 gctaaatcag ttgaagaact agaaatgcta actagtcaca cttttcaatc aaagacgcta
      241 catggctgtt acaggtctaa tcagagatta gtagacttct actctaattt tcagagttgt
      301 ccttacgaga ttgaaagcag agggaataat aaaaacgacc gtggttttat aagttatgat
      361 tgcaaagtta ctaaggagga tttacccaac ataataaaaa atataataaa ggaaaagatt
      421 gaaagcggca ttcatgaaaa agatatttgt gtacttgctc ctcaacacta tcctctgttt
      481 tcactgggtg agtatttaaa gaaagaatta ccgtattgca attttgatgc tataaatata
      541 tcaccaatta aggtaaataa tcatagttta tttttcaagt tagcaatttt gtactttact
      601 gaatcaggtg aaaaggttcg aagaagaaag ctaattgcaa acgatatttt aattatatta
      661 aaaaatgaat ttggaataga agttaaagat tattttattg acttagattt attacagcta
      721 ataaacagtg tgaaaagaat gtatagtgga gacgacaacg gtgttgattt atacaaattt
      781 gttaccaaac atatatttgt taatcttaat attgacgaga aaaattaccc gtctattttt
      841 aagcactatc tattttttat taacgaagta gaagatcgga taaaaaataa ttcattatct
      901 agtagtgtat ctagttttaa gaagatgttt aaatctcgag atggtatcac acttactaca
      961 gcacataaag ttaaaggaaa tgagtatgat actgttatcg ctattaatct tctaaacggg
     1021 aaaattcctc attggaatga aatttttaat gtttctgata aaggaattag ttcagcgaga
     1081 aaactactat acgtaatttg ttcaagagca aaaaataatt tatttctttt ctcagaggtg
     1141 ggttatttta cgagtaaagg agacttgctt tatcccactc aattattaga taaaatagaa
     1201 tttgattata atatttaaaa tacctaggta gtcgttgttt taaatatagt aggaatcgga
     1261 cgctcagtac ggagcgtcta tttttttacc caattttgaa aggaagtgaa cttatgaaaa
     1321 taaaaaatca agaatcaaaa ggtcgctctc ccccctttaa aaccatcaca caaataagat
     1381 tcattcaaac ataaaaacag aaaggatagg taaaaatatg gaacttaaat atgtcattcc
     1441 caatatggaa aaaacatttg gacaattaga atatgctggt gaagggaaca ttgaacaacg
     1501 tcgaataaat ggcagaccaa ccattctctc tcgtagttac aatttatact caacgattca
     1561 acgagcagat gatattgttg tcattcttcc ctccgaagct ggcgagaaac attttgaatc
     1621 cgacgaaaaa gtcaaactca tcaacccaca tattaccgca gaaggttata aaattggcaa
     1681 tcgaggattt accaattaca ttatgcacgc agacgatatg gtgaaagcat aagaaaggaa
     1741 aggtgaaaaa atatgagatt agcagaaggt attgtgattg aaaaagacca gacatttggc
     1801 acattgaaat tttccgcctt gcgtcgtgag gttcgtatcc agaatgaaga tggtacggtt
     1861 tccgaagaaa ttaaagaacg tacctatgac ttaaaatcaa aaggacaagg acgcatgata
     1921 caagtcagta tccccgcttc tgttcctttg aaagaatttg actacaacgc agaagtggaa
     1981 ttaatcaatc ctgtggcaga taccgtagcg acggctactt ttcgaggggc agacgttgat
     2041 tggtacgtga aagcagacga tcttgtatta aaaggacaga caaaacctca tcagaaatat
     2101 aaaaaagaag cgtccgaaag tgaaaaatag gtgttgctta tgacaaacac accattgatt
     2161 ttgaaagaaa ttccgaagga tgaagccatt tcttttattc ggcaatacca ttattcaaag
     2221 atactcccta gattgtgtaa atactttcta gggatttttt cagaagaaaa attattaggc
     2281 gttgtggaat taggctgggg tacgcaaccc ctacaaacca tacgcaaact ctttcccgac
     2341 agttcccttc aaaccacaga ttatttagaa attggaaaga tgtgttttct tccagaaatg
     2401 aatcaaacca attattttgg cagtcaagct ctttcagctc taattaagtg gttgaaagaa
     2461 catacggact gtcatttttt atatacactg gcagatggaa ttgagggaaa atgtggctat
     2521 gtctatcaag cgtccaactt cttttactgc gggtatttca agacaagtgt ctatcgtgat
     2581 aagcagtcat gggaaaagat acaccctcga agtgctcgcc ttttattaga ggaaaatgct
     2641 cgctttgaac aggtagagaa aaagcattgg ctttcccagg cattttgcga atacaaaggg
     2701 attgagaaaa tcaatggacg catgtttcgc tatctctatc cgttgacaaa agaagcgaaa
     2761 aaactgttag gacacactct ctatcgacgt cattattatc cgaaagaaaa aaatttacgc
     2821 tttgaaaagc gaatcgctta tcagaaatat gaagccattt ctcaaccaac ttttgataaa
     2881 caggctcgta tctacaatac acaattattt tgataggtgg aaaggaggat ttttatgcca
     2941 ttgcttcatg acagaggaga gcgaatcaaa gccaacgata agcacttggt ctatcatttt
     3001 gtactaattt cattgctggt agtttttcta gtcgtcctac tcccttttca ttggagagcg
     3061 ttacaatcgg tgaactggca agcagttcag ttagatacat ttttctatca acttcatacg
     3121 ccatactttt ggagcagtat tctcactgct ctttttgtat gcggacttat cagctggctt
     3181 tattatcact atcgtataga ttctataaaa cagttagagc accgtcaaaa gttagctcgt
     3241 atgattttag aaaatcattg gtatgaagcc gaagaaactc aatcagaggg attctttaaa
     3301 gatttaccca gcaagaaaac caaacaacga atccgccatt ttcctaaaat atattatcga
     3361 atgaaagatg gactaatcca tattcatgta gaaatcacct tagggaaata tcaagatcag
     3421 cttttgcacc tagaaaacaa actagaaaca ggattgtatt gtgaattagt ggaaaaaacg
     3481 ttgaaagatt cttatgtaga gtatgtccta ctttatgata ctattggcaa tcgaattggc
     3541 attgaagacg tggtagctca agatggacgt gtcaaattga tggaatcggt ctattgggag
     3601 ttcgattctc tccctcacat gttaattgct gggggcactg gtagtggaaa atcctatttt
     3661 attctcacat tgattgaatc tctcctgcat acaaatgcaa agctttatat tcttgaccct
     3721 aaaaatgctg atttggcaga cttaggggcg gtcatgaata atgtctatta ccgtaaagaa
     3781 gatatgttgg catgtatcaa tcaattttac gaagatatga ttgatcgcag tgaaaccatg
     3841 aaacaacacc ctaactataa aacaggagaa aattatgctt atcttggttt gtctgccaat
     3901 tttcttattt ttgatgaata tgtggcaatg atggatatgc taggtcgtga aagtaccacc
     3961 gttattcata aactcaaaca aattgtaatg ttaggacgac aagcaggctt ctttttaatt
     4021 cttgcctgtc aacgtccaga cgcaaaatat tttagtgatg ggattcggga caactttaac
     4081 ttccgtgtgg ctttgggtcg tatgtctgaa atgggctttg gtatgatgtt cggaagcgat
     4141 acacaaaaac aatttttctt aaaacccatc aagggaagag gttacgtgga tacaggaaaa
     4201 agtgtgatta gtgagtttta tacacccctt gtaccaaaaa ggtacgattt cttaggagaa
     4261 ataggaaaag tcattcaaaa aaagcaatct gagcctgtac cgcacgaagt gaaaggtaca
     4321 ggttcagact tgctttgatt ggtgtggcgt tagccacgcc aatcctttag ccccctctat
     4381 ctaacagggg ggcacaaata caactgaaaa caggcaattc tcatcaacca atgttgatgg
     4441 tacaaaggat tgcggaaaat atgaaaggtg taaactcaag ggtcatagtt tacatctttt
     4501 ttagattgga ggtttgcgaa tggaaaacga aaaaatttgg attaaggaat tgaaagaaaa
     4561 gcgactggct tatggcgttt ctcaaaataa actggcggtt gcttctcata ttaccagacc
     4621 ttacctatcg gatattgaaa caggcaaggc agtccctact caacaggtga aagaagattt
     4681 actgaacgct ttggaacgct tcaatcccga taacccattg gagatgttat tcgactatgt
     4741 acggattcga ttccctacca atgatgtggc tcaaattatc ggtgaggttt tacgtttgaa
     4801 tatggattat atgctacacg aagactttgg ctattattct tatcccgaac actatcgttt
     4861 cggtgatatt gttgtcctag tctctcatga tgtaagtaaa ggtgttttat tagaactgaa
     4921 aggaaaaggc tgtcggcaat ttgaaaactt tctattggct caacatcgga gctggtatga
     4981 tttctttcaa gattgcatgg aacataaagg tattttcaaa cgcttggatt tagctatcaa
     5041 cgataaaaca gggattttga atattccaga tttggcaaag aaatgtaaac aggaagaatg
     5101 catttccgtc tttcgaagct ttaaaaatta tcgcagtggt gaattggttc accgtgatga
     5161 aaaaccatac atgggaaaca cactttatat tggctcatta aaaagtgaag tctatttctg
     5221 tatatatgag aaagattatg agcaatacgt gaaaaatgat gttcccttag aagacgcaga
     5281 agtaaagaat cggtttgaaa ttcggttaaa aaatgaccga gccacccaag ccatgaaaga
     5341 tttattggca catcaacagg cggaaaaaac agcctttgaa attatcaatc gctatatccg
     5401 ctttgcagat aaagacgaca ctaaacgtcg gagtgattgg aaaactaatg aacgttggga
     5461 atggtttatc ggtaaaaatc gtggagcatt acgcctaacc acacagccag agccttattc
     5521 ttttgaacga acattgaact ggttacatca tcaagtcgcc cctacattaa aaatcgcaag
     5581 cattttagat gttctcaacg gtacaaccat tatctccacc atgattcagg aagccaaact
     5641 gacagaaaaa catgagaaat taattgaaca acaacatcta gccatggagg atttgattac
     5701 atgagccttt taacaaaaga attaaagaag ttagggtttc agtgtggcat tgagtttcaa
     5761 gcgtatatac aaaatacagg gaaatatacg tctctcatta ttgagggaaa acggcaagct
     5821 ggtgatacca tctataccta tgatttttac aaggtgagat tttataataa ttataccaat
     5881 cgagtaaccg tctatggaga gcatttaacc ccttttcaat tattaaggcg tgtaaaaagc
     5941 tatatctatt atcgagaaaa atatcttaag gaaaggagga caatcacatg aattttgggc
     6001 agaatttgta caactggttc ttggataatg cacagtcatt ggtattgtta gcgattgttg
     6061 tcattggact gtttttaggt tttaaacgag aattttcaaa actcattggc ttcttgatta
     6121 ttgctctaat cgccgtcgga cttgtgttca atgcagctgg cgtgaaagat gtgttattaa
     6181 acttatttaa ccgaatcatt ggtgcttaac agatattttt catgataaaa taggaagtat
     6241 atattttgtt atgaaaggat ttatgaatat gtttacaaat gatcaattta aatctctttt
     6301 aattaaaaca ttagaaagaa agagctcttc aaattattat gaaggaggta acgagattgt
     6361 ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta cttattataa
     6421 aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag ttccttttga
     6481 tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa gctccatata
     6541 tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc cagagaatta
     6601 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
     6661 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
     6721 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
     6781 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
     6841 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
     6901 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
     6961 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
     7021 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
     7081 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
     7141 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
     7201 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
     7261 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
     7321 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
     7381 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
     7441 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
     7501 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
     7561 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aattaaatta
     7621 ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt aaactattaa
     7681 aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt tacaaacagc
     7741 aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca gaatatgctc
     7801 tgttaaaact agaattttta aaggaaaacg atttttcaga tatgctagct gtctatgcta
     7861 attcggtaaa attatataca gacacgaaaa caataagaga atattttggt atcgaataca
     7921 gtagtaactt gggagatgtt ttatttcaat ttagccaaac tcttgctcga tttattccga
     7981 cagaagttag tgagaagaaa aatgaagacc aaaaagaagc catgtgtttc tcattaagtc
     8041 aaagtgattc agaagatccc agaaaaaaat attgtttttc agttagaaga aatcctttaa
     8101 agggaaatgg tgagctggga aaacgaagtc cttttaatga caataaaacg agattgtata
     8161 gacccagctt atatgaacga ttagggtcag ataccaattt aagtttcaga tattcaatga
     8221 atccagatga tgaagaaact gatgaaggta ttattgcaaa atggacaaaa aataaaattg
     8281 aatagttaaa gcattgaaag agcaatcacc tattttgatt gttctttttc tatttttacg
     8341 gaaaggacgt gaagacatga aaaaaataag aagttacacc agtatttggt cagtagaaaa
     8401 agtgatttat gcgattaatg atttacaact ccctttccca attaccttta cgcaaatgac
     8461 atggtttatc gtttctttgt ttgtcgtcat actctttggc agtttaccac cactttctct
     8521 gattgacgga gcactactca aatacttagg gattcccatt gttctcactg ggttcatgtg
     8581 ccaaaagacc tttgacggaa agaaacccta tggatttcta aagtctatca ttacctatgc
     8641 tctacgccct aaagtaacct acgctggcaa acaaatacga ttaaaaaaag aaacaatgaa
     8701 tgaatcgatt acgattgtaa ggagtgaaca actagatggc atatccgatt aaatatatgg
     8761 aagataatct cgtatttaat catgatggcg aagtattcgc ttattatgaa ttaattccct
     8821 ataattacag ttttttaagt tccgatgaaa aaattcaagt tcatgataat ttctgtcaat
     8881 tgattgccca aaatcgtgat ggcaagattc atgctctgca actagcaacc gaatcaagta
     8941 ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg acgattaaaa gagatagctt
     9001 acgaaaaaat agatgaacag accgaagcgt taatttctat gattggtgac aatcaagtag
     9061 actatcgctt ttttattggt tttaaactct acctcaatga acaggaagta tccgtaaaaa
     9121 atgttgggaa agaaattcaa aaagcatttg cggaattctt tcatggtgtg aatcacaaat
     9181 taatgggcga ttttgtttct atgcctatgg cggaaatcga acgcttttct aagatggaac
     9241 agttgttaga aagtaaatta tccagacgct ttcagattcg taaattggag aaagatgatt
     9301 ttggttatat tattgaacat ctttacgggc aaacaggtgt cccttttgat gactacgact
     9361 accatttacc gaaacgatac gaagataaaa aagtgttggt aaagaaatac gatattctca
     9421 aacccactcg ctgtttaatc gaagaaaatc aacgctattt acggattgaa caggaagata
     9481 ccactactta tgtggcgtat tttacaatta atagtattgt tggtgaattg gatttcccta
     9541 atagtgaaat tttttattac cagcaacagc agtttacttt ccccgttgat accagtatga
     9601 atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt tcgcaataaa aagaaagaac
     9661 tgaaagattt agatagccac gcatgggaaa gcgacaacga aaccagtact aatgtggtgg
     9721 acgctttaga atctgtcgat gaattagaaa gtaatttaga ccaaaccaaa gaatctatgt
     9781 acaaactaag ctatgtggta cgggtatcag caaagaattt ggacgagctg aaacaacgtt
     9841 gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa attagtacga ccttttggcg
     9901 atatgctagg cttgcataat gaatttattc cctcaagcaa gcgatatatc aatgactata
     9961 ttcaatatgt cacgtcggat tttttggctg gtcttggttt cggtgctaca caaatgattg
    10021 gtgaaaattc tggtatttac gtcggttaca atctcgatac aggcagaaat atttatctcc
    10081 aacctagcct tgcaagtcaa ggaattaaag gctctgtaac aaatgcatta gcgtccgctt
    10141 ttattggttc attaggtggc ggaaaatcat tctctaacaa tatgctggtc tattattcag
    10201 tactctttgg cggacaggca accattgttg acccaaaagc
//
LOCUS       DASHWH010000085         9995 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.088, whole genome shotgun sequence.
ACCESSION   DASHWH010000085 DASHWH010000000
VERSION     DASHWH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.088"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..510
                     /locus_tag="U2R41_002396"
     CDS             <1..510
                     /locus_tag="U2R41_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEM8889769.1"
                     /translation="TSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQ
                     IQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTT
                     IAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNY
                     KKRTRRTTK"
     gene            526..675
                     /locus_tag="U2R41_002397"
     CDS             526..675
                     /locus_tag="U2R41_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889770.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            751..1344
                     /locus_tag="U2R41_002398"
     CDS             751..1344
                     /locus_tag="U2R41_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM8889771.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            1357..2256
                     /locus_tag="U2R41_002399"
     CDS             1357..2256
                     /locus_tag="U2R41_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM8889772.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            2259..2732
                     /gene="ssb"
                     /locus_tag="U2R41_002400"
     CDS             2259..2732
                     /gene="ssb"
                     /locus_tag="U2R41_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8889773.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            3098..3358
                     /locus_tag="U2R41_002401"
     CDS             3098..3358
                     /locus_tag="U2R41_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889774.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            3803..4009
                     /locus_tag="U2R41_002402"
     CDS             3803..4009
                     /locus_tag="U2R41_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEM8889775.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4009..4260
                     /locus_tag="U2R41_002403"
     CDS             4009..4260
                     /locus_tag="U2R41_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889776.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            4276..4692
                     /locus_tag="U2R41_002404"
     CDS             4276..4692
                     /locus_tag="U2R41_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889777.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            4674..4946
                     /locus_tag="U2R41_002405"
     CDS             4674..4946
                     /locus_tag="U2R41_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889778.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            5113..5514
                     /gene="tnpA"
                     /locus_tag="U2R41_002406"
     CDS             5113..5514
                     /gene="tnpA"
                     /locus_tag="U2R41_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEM8889779.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            5526..6671
                     /locus_tag="U2R41_002407"
     CDS             5526..6671
                     /locus_tag="U2R41_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8889780.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            6909..7172
                     /locus_tag="U2R41_002408"
     CDS             6909..7172
                     /locus_tag="U2R41_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEM8889781.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            7166..7516
                     /locus_tag="U2R41_002409"
     CDS             7166..7516
                     /locus_tag="U2R41_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8889782.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(7540..8154)
                     /locus_tag="U2R41_002410"
     CDS             complement(7540..8154)
                     /locus_tag="U2R41_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8889783.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            8604..9929
                     /locus_tag="U2R41_002411"
     CDS             8604..9929
                     /locus_tag="U2R41_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8889784.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         3642 a         1594 c         1937 g         2822 t
ORIGIN      
        1 acaagtgccg aaatgaccgc caaatgggaa agttacttat taaagattgg tgagcagaaa
       61 ggaacacaag ctactttctt agctaatatc caaaaatttg tctctcactt gttggaagtc
      121 gtaccgggac aaatccagtc aacagatttt ggatcaacgt tacaagaggt gaaagcagcc
      181 agtgaaaaac aggaagcaac gcgtcattta ggtgtttgtc caaaatgtcg ggaacaagaa
      241 gtcttgcttt atcaaaatgt ggcggcatgt acgtctgaag cctgtgattt taaattatgg
      301 acaacgattg ccaaaaagaa actcacagcc acacaactga aagagatcat tcaaaacggc
      361 cgaacaagcc aatcagtcag aggactaaaa ggtcagaaag gttcgtttga agcaacgatc
      421 gttttaaaag aagactttac aacaggcttt gaattttctg aaaagaaaaa aacgaactat
      481 aagaaaagaa cacgccgcac tacaaaataa tgaaggggtt atcctatgcg aaatacaaca
      541 cacaaacaac aagaaatcga aaatcaaaaa gcaaaaattc gtttagaaca agagaaatta
      601 cgtgcaaata aaagaagact gcaacaatta aagaaccagc agcgcattca aaaagaaatc
      661 gaattagatc gatagacgtt aatcttgtta caaataaact actatgatat atttgatgta
      721 gtgtatttgt aacgaaggga ggggaagtaa atgcaagata aactgaaaga attgttggct
      781 attcgcgatg gaaatttatc tatgaaggaa gccaggaaat ttggaatcgc agcaaccact
      841 gtacaaagat tggtgaatca aggaaaattg atcaaagttg atcgtggtta ttatgtagag
      901 gatggtcatg gaatagatga tttattttgg cttcagcaac gattttccag gggaatcttc
      961 tctcatgaaa cgactttaga tatttaccag ctttctacga atatgccgaa attcattcac
     1021 ttaacttttc cccatggcta taacgttgat cgttcaatta caaaagagca gcagttacaa
     1081 tttcatttcg tgaagaaaga agtttatgaa ctaggaaaag tagagggaac ttcctttcaa
     1141 ggaaatccta ttactatgta tgataaagag cgtacacttt gtgatatttg gaatccgaga
     1201 tatgaatttg aatatgaaat gaaattagaa gcattaaaag agtacatgga agacccattg
     1261 agagatccaa gtaaattaag agattacatg acaaaactac atgtagaaaa agaaatgaaa
     1321 taccacatgc aaagcttata ttgatggagg aaaaagatga caccgacaca atttgcagca
     1381 aaaacaagaa atttagcaaa gagtacagga ttaaatgcac agttagtgca acggcatttt
     1441 atgatggatc gtctaattga acaaatttct gaatcaaagt acaaaaatga cttcattttg
     1501 aaaggcggct ttttattagg atcaaaatat ggaatcgata gaaggacaac aattgatatc
     1561 gatacgacat ttagaaatag caaattaact gaagaaaaat tgaagagcat ttttaatgat
     1621 ttaactagaa aacccacaaa agaaggcatt gttttctcta ttcaaggaat gaaggaaaca
     1681 agagaagccg atttttatcc tggatttcaa attaaattgg tggctaattt agagaaagta
     1741 cgtgtaccgt ttaaaatgga cgtgacaaca ggagattcta tttatcctga agtagataca
     1801 cactatcacc aattaatgtt tgaagataaa aaagtggaga ttccagctta tccaacagaa
     1861 caaattattg cagaaaagct aagtgctacg ttcagctttg gcacagataa ttcgagagca
     1921 aaagattact acgacttatt taccattccg aaactagaaa atatcgatac gaaagagtta
     1981 tacaaaagtg ttcgaaatac atttacaaaa cgtggggata caaaaccgtt gtccttttat
     2041 tatgaaagag aaatggataa gatcagagga aatgactatt tggaaaagca gtgggaaaaa
     2101 tatcgctcag ttaacacgtt tgcgaattcc atttcattta cagatacagt caacgaaatt
     2161 gatcacctaa tgaaaagtgt gatcaaaatt gaaaatcaag aaagaaaacg gcagtttaaa
     2221 agatcacaac aaatgaatca agaaatggag agataaagat gattaataat gtgacattag
     2281 taggacgctt aaccaaagat cccgatttac gttatactgc aagtggcaca gcagtagcga
     2341 cttttaccct agccgtcaac cgaaatttcg cgaatcaaaa cggcaatcga gaagccgatt
     2401 ttattaactg cgtgatttgg cgcaaaccag cagaaacaat ggcgacatta gctaaaaaag
     2461 gcggcttgat cggtgttgtc ggacggattc aaacacgaac ttatgacaac cagcaaggac
     2521 aacgtgtgta tgtgactgaa gttgtagcag ataactttca actattagaa agtaaagcag
     2581 cgacagagag ccgtgcgcac gctgatcaat caaccaccag tccaagcccc actacctttg
     2641 aacaacggga tacaaacaat ggattggacg cttcacaaaa tccatttggt ggacaatcaa
     2701 tcgatattag cgatgatgat ctaccattct aagtagcatt ttctattggt tgcatttgct
     2761 acggtttatg ttataattta gtcaacaaaa agaattttca atctataacc aaaaaccact
     2821 agctggcggg ctagtggttt tcttttacgt ttctattata agaattaacg ggtaggctta
     2881 ctgtctttct tggcaaattt acaatcaagc catttgcaga tatagtgcgc ggctacttct
     2941 gccatgattg caaaaacaaa atcaaaaaga attttcaaac ctataacctc ctccccttgc
     3001 cagattaggc cggggtagca gacagcaaga ctattatagc ataattgaaa tattcaaatg
     3061 attggatttt tgctataatt ttagcgaggt gattgtgatg aacaaatatg ttggtttgct
     3121 tgataaaatc cgagtaataa aaacgcaacc gttattggtt cgctttactt tacaaacgat
     3181 tcatgaatcg attaattgtg tagttgcaga tattgaaatt atcgataaat tgctaattat
     3241 ggatgatgga aaatacaata ttgccgtcac aggtcacttc aataaacgaa accagctagt
     3301 tattgctagc atgtatgtta gaaatcccga tcagtttacg cgattgatgg gcatataaaa
     3361 aaagaagagt tgcaatccta atgtaggact gcagctcttt ttgttttaca tagaaacaag
     3421 aaaaatgaaa aggccttttt aggaaggcgc gagcgccttc tcaattgaaa aacctctctc
     3481 actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt tcactaagat
     3541 aaagggtctt gcaaagcaag acaaaataga aaaaacttcc tctcaccaaa aggttcgttc
     3601 ctgttcgtta tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa
     3661 aagcgacaag gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt
     3721 gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc
     3781 ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag
     3841 cgaattattc ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc
     3901 gaaatgtatc aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt
     3961 gtcttatggc gattatattt tgattgatga gaaaggaaag aaacgttgat gaaaacacaa
     4021 cacaaattat tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct
     4081 gaaaaagtag cagaagcttt acttattccg cggtactgga cgacagaaac ccctttagta
     4141 gaggatgcac cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat
     4201 tatcaagaaa acttggaaac ttatgaaaac gaatattggt taaccgaatt agagaactaa
     4261 aaaggagaaa aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat
     4321 aaaatggcac aaaaaatgga ggatatgaca ttcctataca atgagacacg tgttccaaaa
     4381 aaacattata aggaaagatt gtcggtagct gtggaagaaa tgattgaatc aggtgtcgaa
     4441 atgaatctca ttgcgacgta ttatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa
     4501 tggtttttcc aagccttact atgcttggaa gtaggcgtca aaccaagcac aatcaaacct
     4561 tctgaatacc aagctttaga actaacgtat gcgaaattta tcgaaacgaa aaaagctaag
     4621 actgtttcaa gtgaatggtt agattatttt gaaaatatca ataagtatgg cgcatgttac
     4681 atcatgaagt aatcagacgg attcaaaaag actttagttg tatggaagaa atgaagtact
     4741 ttgttcaaaa tgttgaagaa cagttatatt tggaaagcga aacgagtcaa acaaaggtca
     4801 cagtcagcca taatcgttta gatgtgtata aacgtcgtcc tgaatcatta aaaaaaggag
     4861 cggagcttct acgactagaa acagaaaaat acgtcatgaa tgtttaccta gaaaaaagtg
     4921 agttggtcga aaattttatg gactagccga gaacacccgt gattttaatc atgggatgaa
     4981 tcggattcag ttgtgaccta gctttagcta ggttaaacaa ctgacaaata taaactaaat
     5041 aatagtgaaa tgtcttgcaa taattgattt tgtagaaata catatattaa aataattgcg
     5101 aggtgttttt gtatgtcaga aattcattat ggacggggtt atgtctattc tattcaatat
     5161 catcttgttt ggtgtgtaaa atatcgacat gatgttttgt atggtcaggt agatattgat
     5221 gtcaaacagt ttttgaaaca aattgctgat gataataata taaaaattat agaaatagaa
     5281 tcagataaag accatattca tttactcatt gaatgcaccc cacaacacta tattcctagt
     5341 attgttaaag tatttaaagg cgcatccgct agactgctgt ttaaaaaaca tcctgaactt
     5401 aaacaactcc tttggggtgg acatctttgg aatccaagct actttgtagc tactgttagc
     5461 gaaaatacag aagaacaaat tagaaaatat atacaaaatc agaaaaagaa atgaggtgaa
     5521 tccgtatgct acaacatagg gcttataaat ttagaatcta tcctaatcaa gaacaagaaa
     5581 tattaatgac taaaactatt ggctgttcaa gatttgtcta taattatttt ttgaatttgt
     5641 ggaatcatga atatacgact actggaaaag gattatctta taattcttgt tctgccatgc
     5701 ttccacaaat gaaaagaaat gcagagacaa tttggttaaa agaagttgac agtattgcga
     5761 ttcaatcctc attgaaaaat ctttctgatg ctttttctcg cttctttaaa aagcaaaata
     5821 aacgaccaca atttaaaagt aaaaaacatc ctatacaaag ttatacaact aaaaatgtga
     5881 ataatagtat cgaaattaga aacaatttcg taaaactacc taagttagga tttgttaaat
     5941 ttgcgaaaag tcaagagccc aaagggcgaa tcttaaatgc tacgattcga aaaaattcga
     6001 gtggtaaatt ttttgtttcc atcctgtgtg aagaagaaat agttgagttt cctaaatcta
     6061 attccgctat tggaatcgac cttggtataa ctgattttgc aatcctttct gatggtcaaa
     6121 agattgataa caataaattc acgtcaaaaa tggcaaaaaa actcaaacgt gaacaacgta
     6181 agttgtcaaa acgtgcatta ctagctaaaa agaaaggcgt caatctcttt gaagctaaaa
     6241 attatcaaaa acaaaaacga aaagtagcac gactacatga aaaagtaatg aatcaacgca
     6301 atgacttttt aaacaagttg agcacagaga ttatcaaaaa ccacgatatt atctgtatag
     6361 aagacttaaa tacaaaaggt atgttgcgta atcataaact agcaaaaagc atttctgatg
     6421 tgtcttggtc tagttttgtg actaagttac aatacaaagc taactggtac ggtcggaaaa
     6481 tcatcaaagt agataaatgg tttccatcta gtcaaatctg ttcaaattgt ggacacaaag
     6541 acggcaagaa atctctagaa attcgagaat ggacttgtcc tgtttgtcat gctcatcatg
     6601 accgagatat caatgctagt atcaatattt tgaccgaagg tctaagatta catgtcatgg
     6661 gattagctta gatccaatca tcaaaaaccg taggaactac ggggatagct tggtaaataa
     6721 gagaaaccgc tgtgagtaaa gaaatacgct tgcaagtatg ctctattccc aagaatctcg
     6781 tgactttagt catgagaggt tcaaggattc atggaatgtt aatctaattt aatagaaaag
     6841 gataattaag tatatacgtt ttttcttatg tatgttatac tatgtatata cagaaagagg
     6901 tgtctactat ggaaatgtta cctataaaaa aatggggaaa tagtaatggc cttagattgc
     6961 caaagtatat tatggaatat cttggaattc atacagaaga caaagtgaag ataatacaag
     7021 aagaagtaaa cggtcagaag cggctaatta tcgaagcggc taatatagaa aatgaattaa
     7081 caattgaaca gttatttgaa aattacactg atgaaagaaa tcatgtgacg attcaaaatt
     7141 taggtgaagc agtaggtaat gaaaaatggt gagaaaacct agacaaggcg atattctatt
     7201 attgaataca gcacctcgat ctggacacga acagacaggt aaaagacctt atattgtttt
     7261 aagtcatgat attattgcgg attatagtaa cgttgttaca gttgctccaa tttctagtac
     7321 aactagaaac tatccgctat atgtaaatat aaactcagaa tatgaaatga agacaacagg
     7381 aaaagtatta ttagatcaac taacgacaat tgattatgaa gccagagagt gtattttttt
     7441 ggaaaaagcg catgatagtt tagttgagga gttattgatg aaagttagaa cagtttttca
     7501 aaaagtaagc aaataaaata gaaacagcag catgatcaac tattcgtgat tgaccatgct
     7561 gtcttttttt attctatata cggtttgtct cgtaacattg tagtcttttg caatcttagc
     7621 gatcgcaaca ccttttttta gatcctcaac gatgttttta taaacaagac gacgttgggg
     7681 atcttttgca ttggcactgt atagcttagg acgtccttta taaacaccgt tcgcttttgc
     7741 aatcttaatg ccttgtgcct gacgtcgttt agattcggta cgttcttgtt cagcgatcat
     7801 ggccaaaatt tgaatgatca aatctttaat gaatttatcc aggagaggat tgccaattgc
     7861 ttcggccatg atcggtagac ttgtgatgat cagctgcaca tttttcttct tcaagtaatt
     7921 gactgaagcg atgatctcgt cgtaatttct tccaagtcga tcaatagctt ccacaataaa
     7981 aatatcttga tctcggacga aatccagtgc ttcttgaaaa acaggtcgat gagtaatcgt
     8041 tgcgccagat tgtttctcaa tgaaaattct ctctgcaccg aattttttca tttcttcaat
     8101 ctgccgttgt tcattttgat cgatggatga gacacgtatg tatgcaactc tcaaaatttt
     8161 tacctacttt ctgcaactat ttaaaaaaca ccctaatcat acacgagaaa accagatttt
     8221 tcaatgtcgc ggtcgtgtct agttagggta taccttatat agacaaaaac taatgatgga
     8281 ggaaatagta tgagaatttt ttgtaagtgt ctactgataa cttattctca aatgttgtaa
     8341 ggattttatt catttgttaa tattaaagaa cggaactcca aaaaagtccg tactctaatt
     8401 tgcagtctct cactctttta gagtgagagt ttttattaga ttttcaaatt aattagtaaa
     8461 gtgatagaat aaattgatca tacaaatcaa aagaagaagc agggtgatag catacctatg
     8521 cttctttttt tatgtattta gctatttctt ctttacaata tcgaacatat gtttgtataa
     8581 tgtgaaagaa tggagtgtta tttatgatgg attattcaaa agagccagta aatgattact
     8641 ttttaatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaaga aatttagatc
     8701 cattaacaac agaacttgtt gttatgagtc gagctgataa tactggttca ggattgatat
     8761 tagcttcttc tcctgaagca aaaaagcggt atggtattac aaatgtgagt agaccacgtg
     8821 acttaccaca accatttcct aaaacactac acgttgttcc accacgtatg aacctatata
     8881 tcaagcgaaa tatgcaggta aataatattt tcagaagata tgtggctgat gaagatctac
     8941 tgatttactc gatcgatgaa tcaatcttaa aagtgacccg atcactgaat ctttttacga
     9001 ctgaaggaac acgaagtcaa cggagaaaga agctggctca aatgatccag gagaatatta
     9061 aagaagagct aggattgatt gctacagtag gtgtcggaga taatcccctt ttggctaaac
     9121 tagccttaga caacgaagca aagcataatg aaggatttgt tgcggaatgg acgtacgaaa
     9181 acgttcctga aaaagtatgg aatattcctg aaatgacgga cttttgggga attggctctc
     9241 gaatgaaaaa aagactcaat cagatgggga ttatgagcat tagagatctt gctaactgga
     9301 atccttatac gattaagaat agactaggtg tgattggctt acagctgtac ttccatgcta
     9361 acggaatcga tcgaacagat atagcaatcc cgccagaacc gacaaaggaa aaatcttatg
     9421 gtaattcaca agtgttacca cgagattata ctagaagaaa tgaaatcgag ctggtggtaa
     9481 aagaaatggc ggaacaagta gccattcgta tcaggcagca taattgtaaa acaggatgtg
     9541 ttcatttaaa cattggtacg tcgatcttag aaacgagacc aggattttca caccagatga
     9601 aaatacctat cacagataat acaaaggaat tgcagaatta ttgtcttttc ttgtttgata
     9661 agtattatga aggacaagaa gtaaggcacg ttggaataac ctatagcaag cttgtgtaca
     9721 ccgattcgct tcagctggat ttatttagtg atccacaaaa gcaaattaac gaagaaaatt
     9781 tagataaaat tattgacaag attcggcaaa aatatggttt tacgtcgatt gtacatgctt
     9841 cttcaatgtt agaaagcgct agaagcatta ctagaagcac acttgttggt ggtcatgcgg
     9901 gcggaaatgg gggaattaaa aatgattgat tacaatgatc gtggacgttt aaagtgggca
     9961 gggttttatt tatctgatca tactgaagaa attga
//
LOCUS       DASHWH010000086         9977 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.089, whole genome shotgun sequence.
ACCESSION   DASHWH010000086 DASHWH010000000
VERSION     DASHWH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.089"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..162)
                     /locus_tag="U2R41_002412"
     CDS             complement(25..162)
                     /locus_tag="U2R41_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEM8889785.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="U2R41_002413"
     CDS             complement(159..1343)
                     /locus_tag="U2R41_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM8889786.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="U2R41_002414"
     CDS             complement(1599..1853)
                     /locus_tag="U2R41_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889787.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="U2R41_002415"
     CDS             complement(1878..3020)
                     /locus_tag="U2R41_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM8889788.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="U2R41_002416"
     CDS             complement(3080..4432)
                     /locus_tag="U2R41_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8889789.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="U2R41_002417"
     CDS             complement(4503..4847)
                     /locus_tag="U2R41_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEM8889790.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="U2R41_002418"
     CDS             complement(4857..5231)
                     /locus_tag="U2R41_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8889791.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="U2R41_002419"
     CDS             complement(5244..5558)
                     /locus_tag="U2R41_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8889792.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="U2R41_002420"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="U2R41_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM8889793.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSSVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="U2R41_002421"
     CDS             complement(8978..9640)
                     /locus_tag="U2R41_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889794.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2691 a         2253 c         1704 g         3329 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccagaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacactagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcatctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gactttt
//
LOCUS       DASHWH010000087         9964 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.090, whole genome shotgun sequence.
ACCESSION   DASHWH010000087 DASHWH010000000
VERSION     DASHWH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.090"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            8..81
                     /locus_tag="U2R41_002422"
     tRNA            8..81
                     /locus_tag="U2R41_002422"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="U2R41_002423"
     tRNA            101..173
                     /locus_tag="U2R41_002423"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="U2R41_002424"
     tRNA            201..274
                     /locus_tag="U2R41_002424"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="U2R41_002425"
     tRNA            288..361
                     /locus_tag="U2R41_002425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="U2R41_002426"
     tRNA            385..474
                     /locus_tag="U2R41_002426"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="U2R41_002427"
     tRNA            487..560
                     /locus_tag="U2R41_002427"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="U2R41_002428"
     tRNA            579..654
                     /locus_tag="U2R41_002428"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="U2R41_002429"
     tRNA            656..726
                     /locus_tag="U2R41_002429"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="U2R41_002430"
     tRNA            740..813
                     /locus_tag="U2R41_002430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="U2R41_002431"
     tRNA            838..925
                     /locus_tag="U2R41_002431"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="U2R41_002432"
     tRNA            947..1018
                     /locus_tag="U2R41_002432"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="U2R41_002433"
     tRNA            1041..1130
                     /locus_tag="U2R41_002433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="U2R41_002434"
     tRNA            1143..1216
                     /locus_tag="U2R41_002434"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="U2R41_002435"
     tRNA            1235..1307
                     /locus_tag="U2R41_002435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="U2R41_002436"
     tRNA            1311..1383
                     /locus_tag="U2R41_002436"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="U2R41_002437"
     tRNA            1388..1458
                     /locus_tag="U2R41_002437"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="U2R41_002438"
     tRNA            1471..1544
                     /locus_tag="U2R41_002438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="U2R41_002439"
     tRNA            1556..1627
                     /locus_tag="U2R41_002439"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="U2R41_002440"
     CDS             1893..3086
                     /locus_tag="U2R41_002440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEM8889795.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="U2R41_002441"
     CDS             3216..4688
                     /locus_tag="U2R41_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8889796.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="U2R41_002442"
     CDS             4771..5559
                     /locus_tag="U2R41_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEM8889797.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="U2R41_002443"
     CDS             complement(5626..6294)
                     /locus_tag="U2R41_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEM8889798.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="U2R41_002444"
     CDS             6409..8121
                     /locus_tag="U2R41_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889799.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="U2R41_002445"
     CDS             8124..9896
                     /locus_tag="U2R41_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889800.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DASHWH010000088         9897 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.091, whole genome shotgun sequence.
ACCESSION   DASHWH010000088 DASHWH010000000
VERSION     DASHWH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.091"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(171..521)
                     /locus_tag="U2R41_002446"
     CDS             complement(171..521)
                     /locus_tag="U2R41_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEM8889801.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVAIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(570..1013)
                     /gene="nrdI"
                     /locus_tag="U2R41_002447"
     CDS             complement(570..1013)
                     /gene="nrdI"
                     /locus_tag="U2R41_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8889802.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1160..1825
                     /locus_tag="U2R41_002448"
     CDS             1160..1825
                     /locus_tag="U2R41_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8889803.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            1815..2270
                     /locus_tag="U2R41_002449"
     CDS             1815..2270
                     /locus_tag="U2R41_002449"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEM8889804.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2420..3799
                     /locus_tag="U2R41_002450"
     CDS             2420..3799
                     /locus_tag="U2R41_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEM8889805.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            3936..4778
                     /locus_tag="U2R41_002451"
     CDS             3936..4778
                     /locus_tag="U2R41_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8889806.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            4852..6300
                     /locus_tag="U2R41_002452"
                     /pseudo
     CDS             4852..6300
                     /locus_tag="U2R41_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6415..8187
                     /locus_tag="U2R41_002453"
     CDS             6415..8187
                     /locus_tag="U2R41_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8889807.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8162..9454
                     /locus_tag="U2R41_002454"
     CDS             8162..9454
                     /locus_tag="U2R41_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HEM8889808.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         3455 a         1601 c         1875 g         2966 t
ORIGIN      
        1 agatgggcta cttttgaaac aaagtgtgat tgattgaaat aagcttctat catcacgagt
       61 tcagttggtg taagatgggt ataggtcatt tgtactcact ccttataatt ctttcgacgg
      121 aactattttg agtgtagcac aaatgacttt tttagttgtc tagcttaatt ttagaattgg
      181 cgtatgctta aaatgcggtt ataggctttt gtatgaacca tttcagtaaa ttgaaaatta
      241 tttatgattg caatctcttg ttgggaacgt gcactatctc gaataatttc tcctaattca
      301 ttggattgat aggtagcgat attagtcaaa agaatcaaca agcgattgag ctgctcttgt
      361 tcttgtaatt gtaaatttgc ccattttgat cgatcatttt cgatggcgac acgtgtatcc
      421 aaccaaaaaa gtgaggttgc tctttcccat acaacgttgt ctaattcatc ctcaatgaca
      481 ttccagttaa ttgctttata gtgtaaataa ttatttatca tagtacctcc taaaatattg
      541 tttgaaaggg gatgatatgg tgtcattttt tattgcattg tttctgcaat tctctctata
      601 tcttctgtcg taccttttag ttcaaattca tccaacacgg taaaaccaaa atcatcggcg
      661 tattgatagg ctgttaggca aaattgttta ttgaagtttc tgttgccact accaataatt
      721 cctaaacaat agtctcggtt tttattgtaa gtaatcactt ggcgcaatgg tgttgtcaaa
      781 atttcgacat ctcctgtttc aataccattt ccaccttcaa gataagtagg cagaaataca
      841 aaataggatt cattgatgtc aaaagtgagt ggttttccta atttgactaa atcttttaca
      901 ttcacataat ctaccttctt ttgatattgt ttttgacaat acgtatctaa atctgcgaga
      961 aaactcgtgg tatttccact taaactaata taatagactt taattatttg catgtatcac
     1021 acctcttttt aataaataac cacatatata gtgcttgttt taattagaaa cgctatatat
     1081 agtgtttatt gtacatgaca tatcgttata gaaaattgac gatggtcaat tattttgatt
     1141 ttagaggaga gaaaattaca tgaaaaattc aggttgttta tgcaccgttc cactcttcgc
     1201 ctctctttca ttagatgaaa aaaatgagat aaagtgtttg cttcaccatc agacgtttaa
     1261 aaaaggagaa attatttttt caccaagtag tgaagaacaa ctgttgatta tatctttagg
     1321 ggctatgaaa atatatcgaa ttagtagaac tgggaaggag caattaattc gtatcgtaaa
     1381 agaaggggat tacgacggtg agaactatct ctttggtcta cccaatgact gcctatatgg
     1441 tgaagctgtt gaagaaacca aaacttgcac tctctataaa tccgacttta ataaagtgat
     1501 ggcacgctat ccacagctca gttatcgatt gttacaatta aatgcttgca aaacagtagc
     1561 tgtggaaaaa cagttgcagc ttctagcgtt tgatcgtata gaggaccggt tagccttata
     1621 tcttaaccaa ctatctatag cagcaaaaat gggggagcct attactttac ctatctctat
     1681 gagagagttg gcaaactatc tagctaccag tcctgaaact atttctcgta aattaagaga
     1741 ttttcaagaa agacaattga ttactagaaa aggaaagcag attacactat tagaagaatt
     1801 ttggtttata tttaattata tataaagaag aagatattaa aattgggatt gtcattgcac
     1861 gattcaatga ttttttaact aaaagcttac taagtggttg tgaagatggc cttacgcgtt
     1921 caggcgtttt gacagaaaat atcgatgttg cttgggtgcc gggggcctat gaaatcccac
     1981 ttacagccgc aacgatggct gaaacaggta aatatgatgc cattattacc ctaggtgcgg
     2041 taatcagagg agctacaagt cactttgatg tggtggttaa ccaagcgtca agtggggtat
     2101 ctcaagtagg cttaacccat aaattaccgg tcatctttgg tatcttaacc gttgaaaaca
     2161 tcgaacaagc cattgaacgc tctggtacta aagctggtaa ccttgggttt acttatgcaa
     2221 cgaatactat tgaaatgatt aatttattca aagaaattaa acaacaataa gtcatcattc
     2281 tatatgaaaa tatctgtaac agcatagggc tagctttaat tttcaatcat ggtaagatgg
     2341 aagataggaa aataaagcgc attcaattaa ttatcaagcc tttctgatga tttgcgcaaa
     2401 aagcttaagg agacttcata tgacgattca atttaaacaa acattagcgc caaccaagga
     2461 taattctgga aaaccaatca ttttaagtga cgctaccttc gctcaacgta atgagaaagt
     2521 ccttcagtta atgcgtgaat atgacttctc atcattgatg atttatgcag ataaagaaca
     2581 cggtagtaac tttgaatatt taactggttt tattccacgg tttgaagaag ctatccaagt
     2641 aatcaaccaa gacggaacgt cgacactgat tttaggtaat gagaattaca ataaaaccag
     2701 tatggcacgt acacaaagtg aaggtattct ttgcccattc ttctctttac caaaccaacc
     2761 tatggatttc actaaaactt ttgctgacta tcttaaagac gttaaagtag atgcttcaaa
     2821 acgaattggt ttagtagact ggaagctttt atctaatgat atggacaact tccacaacca
     2881 tcattcaata ccagcattta ttgtagatgg tatacatgaa gttttcggtt ctgacaaggt
     2941 agtgaatgca acccaactat acatgcatcc aaaagaaggt gctcgtgtga caaacaacgc
     3001 agaggaaatc gctcgttatg aatatggtgc ttcccttgct tctgatgctg ttcttgaagc
     3061 aatgaaccag ctaaccgttg gccaaagtga gttagcagca ggtaatcact taaatcgcga
     3121 tggtcaatat ccatctgttg tgaccattgc agcctttggt gatcgctttg agggtgctaa
     3181 tatttatcca actgataata cgctaaatga tggcgataaa gttgcattaa ctgtagccta
     3241 taaaggaggc ttgtctagcc gctctggcta tgcagtaaaa gaggaaagtc agctcgctga
     3301 acatgatcca ggatacttag aagaagtggt ttacccatat ttccaagcct ataaatggtg
     3361 gctagataat ttaaccattg ggagaaaggg tggcgatttc taccaagcat tcagtgactt
     3421 ctatccgcaa gctacttatg gctggaattt gtgcccaggg catttagtag ctgacgaaga
     3481 atggttaagc tcaccatttt atcaagactc tgatgcaaca attcaaagtg gctacatctt
     3541 ccaactagac tttattcctt cacaagcagg ccacaatggt gtttcagctg aatcaacgat
     3601 tgcggttgcg gacgcggcac tacgtgaaca gattaaggct gaacaacctg aattatgggc
     3661 acgtatctgt aaccgaaagg attatctaaa agaagcatta ggtttagaat tatcagaaca
     3721 tttactaccc ttagcgagta cctttgccta ctatagacct ttcttattag acaaaaaaac
     3781 tgctatctac tttggataaa aaaatgcccc tcaagattgt ttgccaaact tgagggtttt
     3841 ttgtctattt ttaagtgaat aatgacggac tatccaaacg gaatcgccat aaagatgtta
     3901 atatatgtat aatattataa agaaattgga ggacaatgcc cttggacgag cagattgaat
     3961 tattatttac catagatgaa ggctatttga atcccttaaa agtggcttta acatcgattc
     4021 gtcagaataa tccaggccaa gctttccgca tttggttgat tcacgaatcc attgcaatgc
     4081 atacgattcg cgaattgcag aagctaacgg attatctaca gtttggtttt gaagcgatta
     4141 aaattgatgg tagtcgatgg aatagcgcta aaactgaaga tagataccca aaagagatgt
     4201 atttccgcct gcttgcgggt gaaatattac ctaaagaaat gaaacgcgtg atctatttag
     4261 acccagatat tttagtggtt aaccctttat tagatttgtg gcaaacagat ttagaaggtt
     4321 atatgctagc agctgctaca catgttggat tgacggacgt ttctacacga gtaaaccaaa
     4381 tgcgacttga cgttgaccat gcttattaca attctggtgt gatggtgatt gatttagata
     4441 aagctagaga aattattaaa tggtcagata ttgcacaaat gattgaaaaa tataacttgc
     4501 tattaatcct acccgaccag gatatcctca accgattata tggtaaatac actaaggaaa
     4561 ttccagaaga aatttggaat tatgatacgc gtaaatatat gcggtatttc accaaaagtc
     4621 tcgctcaaca tgatattcac tgggtgatgg cgcatactgc tattctccat ttctgtggtg
     4681 gcccaaaacc atgggacgat aaacatgata atcgttttac cagtctttat cttacctatc
     4741 aaaatcaatt aaagcgtatt gaagatttga tgaagtaaag tgaaacaagt gtttttcatt
     4801 acctacggtg gtttaatgct tatttattat cgttaaatca tgcttataca ctttacaaaa
     4861 tttgattttc gaaatggccg tccaacgaag tctgcgatct taaccacgag ttccattgac
     4921 atggccaaaa agtattatcg agcgatcaaa gaaatgacga aagaacctga ctggttaaca
     4981 aaagagtttc ctaatcagcc cattcgtgaa ggaagaacga tggaggatcc tgattttcca
     5041 cgaatcgcta ttacctattc attagaagaa aataaccaag attcaagtgc ccaacaagaa
     5101 gaaatgaaaa aaattattga ggaatataat cagtattttg atactgcctg gtcattagca
     5161 gacattgagc gttataacgg agacatcaat aattgtttgg cacgaaaaag agcggaattc
     5221 aaacaatttg ggaaacaaat cgatttagta atcgtagtgg atcgtctatt gacaggtttt
     5281 gacgcaccta cgattcaaac cctatttgtg gatcgtaatc tagagtatgc aggattgatt
     5341 caagcttttt cacgtacgaa ccgtacgtat cctgaaaaga caaaaggatt gattgtgacg
     5401 tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg cgacaaaact gtattcagaa
     5461 gcaaaagaag aatcgggttt gatttatcca agttatcagg aatccaaaaa aagattcaag
     5521 caagcgtatg gtaagttaaa ggaggtttca tccatgaaat ccattgatga acatacgcca
     5581 cttgaaatcc gtatcgagta cgttaaagca tttcaagaat tgaatcgtgc gtatgaatcg
     5641 ttagtgactt atgacgatta caatgatgac atggtgcagt cttcaacttt gaacaatcaa
     5701 attcaacttt tggaagaaca aattggcgtg tacgagaccg tgaaggggtc attaatagag
     5761 gaagaaccag aaaaagaggg agaagatttt tcaacgatcg aattttatag tgaaaatacc
     5821 actaagttgt atgatattga ttctacttat attgatcaat tactaggaac ttatgctgcg
     5881 aatagttcag atgcacgaga tgaaattgaa aaagctttgg ctaaattgaa taaaacagaa
     5941 ggagtcaaag aagtttaccg acaaatttta aatgcatttg atgtggggac tttagatagc
     6001 aatgaagata tttttgtcat taaacgtcgc tattttacac aagcaagtga tgaattgatt
     6061 catgcctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
     6121 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
     6181 gaaggatata aagccaaaca tccaaaagcg aagccattta aatacccaca agaaatgaag
     6241 cgagcctggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
     6301 acgagaaaat tgaagaaatt gtatgataaa catgttaata aatgatgctc gaaataaaat
     6361 ttggttttat ttttacattg atacggtaaa taaaatttga aagtagaaga aaatatgaac
     6421 aacaatgaaa caaaaaaaac atatgaacta agaataactg ctgatgagat taaatcaaca
     6481 gacccaacat ttactatgaa gaatctaagc ttaggggata attatcaacc aaaagatgct
     6541 tacttccgta cggcagaagg cgaaataact agaagaaact acggtaaaaa tgaacggaaa
     6601 atttcaaatc aaacattacc gcgattagct attcaaatgt ttgaacaatc gattgtttct
     6661 ctaccagaaa gcgaaagagc aacatttcct gtgtgtcgat atcactcagg agatgagata
     6721 atacgaggga tttttccaga tgttgacagt ttcaaaaaaa aaaggaagtc cattgaattt
     6781 ttgatttata aatataatga agacaagcag atagtcatct attgttggag tattttttca
     6841 actttattat ttgtacaaga atgtttgaaa cgttttggaa catctgggga tgaatttatt
     6901 cttgtttata aagggaaaga gaaaaaggaa atacgagtag gagagactca gactatgacc
     6961 aatcatttgc tgaaatcata tgttttacat ttactcaaat ctaaaaacct tattctacgt
     7021 ggcgctcctg gaacagggaa aacattcctt gcaaatgaaa tggcagctga tgtcgtttca
     7081 aatggcagaa ctacacaaat caatgaattg agcgaagatg aaaagagccg aattggattt
     7141 gtccagtttc atcctagcta tgattatact gattttgtcg aaggtttacg tccaactaca
     7201 tccgatgatg gcgttgttag tttcaccttg aaagatggaa gctttaagag ctttgttgaa
     7261 aaagcaaaga aaacaaagaa agtaaatgga caagataatt ttgaagaagc ttgggctaaa
     7321 tttttcgatg cggtaacgga agctagtata gagggagaag gctacaataa acttaaaaca
     7381 ttgacaggta aaccagtacg caatcttcta gcctatgaaa gaaatgaaat tcaaggtgtt
     7441 tatcctgcta aaaccaatac ttatctaaac catgaccaaa tttataatgt ctatcgtgga
     7501 ttgccaggta cgccaaaagg tggatttgat gcttatcgaa aagcgattgt aaaacattta
     7561 aaagaagaat ttggcttaaa aaactacacg gcgtcaacgg tagattctac gaataaccaa
     7621 aactatgtgt ttatcattga tgaaattaat cggggcgaaa tttccaaaat ttttggcgaa
     7681 ttatttttct caattgatcc aggatatcgt gggcataaag aaggtgttta cactcagtat
     7741 gcaaacctcc atgaaaatcc agaagaaaaa ttctatattc ctaataatgt ttatattatt
     7801 gggacgatga acgacattga ccgttctgtt gacacttttg attttgctat gcgccggcga
     7861 tttactttct tagaaataaa agcagaagat tcagcacaaa atatgctgac tcaagagaaa
     7921 acaaaagaac ttatgacgcg tttaaatgag gcgatcgttg ccaaagaaaa aggtggattg
     7981 tcaactgatt accaaattgg tgctagttac tttttaagta tcgataatgg tgaggagaca
     8041 atggaacaat tatgggatac taagttatat ccattattga aagactattt tcatggtgaa
     8101 cataaggcga aagaaaaatt agaaatacta gaaaaggtat actctaatga cggtgattta
     8161 gatgaagctg aaacctctac acgataatca agaaatcaat gactcagttg tgcaacatcc
     8221 aatcattacg aacttagtta atcgtgactt aagtagttta gagaaagaaa attttattat
     8281 ctttccgcaa cagttaaatc aatccaagga tctaagtgaa gataacttga tatttcaatc
     8341 aagaaatgga aaaatttgga cttgtaatgt cgtaggtatc cttagcgatt caacagatga
     8401 attaagaatt cattctagat tcgctaatca aatgaagcat gaagatttct ttttacgtta
     8461 tatgcttcag cgtgttttga attacaacgt gattaatgat cattttagtg catctaaaaa
     8521 gatgacatac tacgatttgt tggtatttct ctttccttat tatctgaatg aagcaatgcg
     8581 taaagggaca tacaaagaat atgtcaaacg agaatacaat aatgccaata tcaagggagc
     8641 tgttgatgtt gcaaaacaca tccgttcgaa tgttcctttc gttggaaaag ttgcttatcg
     8701 aacacgagag tttagctata ataatcacct aacccagctt attcgtcata ctattgaaaa
     8761 aatccaaaat gagtacgatt tcttacttag tggagatgag gatacgaaag aaaacgtggt
     8821 tctaatcaaa caaacaacac ctggttatgc tagattaaat caatttaata tccttcaaga
     8881 gaatatcctt catccagtca aacatagtta ttacgaggag tattcggctc ttcaacaaat
     8941 ctgtatccaa attttatcgg aagaaaaatc aggatttgga agtgataaaa atcaaataca
     9001 tggaatcatt atcgatgtgt cttggctatg ggaagaatac attggaaaag tcactggttg
     9061 gaaacattat ggcagagata aagggttagc aacaatgcat ttatttcaag agcccaaccg
     9121 gtccccacga tatccagatt ttacatttaa taatatacca attgatacaa aatacaaaaa
     9181 acatcttgat actagaaatg actataatca gttagtcaca tatattcata taatgaattt
     9241 agaccaagca gatacaatga aaggcggctt tctgcaacct acttccgatc cccttcctgc
     9301 aaatcatggg tataaaaaaa taggcatatt aagcgggcta ggaggagaaa tattcacata
     9361 tcattttttc attccgcaag ctgcgtcaag ttatttggaa tttgtagaac agatgagaga
     9421 ggtagaagca gcactacaaa ctacttttca ataagtagga attatagaaa ttttactcaa
     9481 aaaatcttca ttaaaaaatc tgactaaata agttgagaaa acagaatata cttcaatatc
     9541 cagtcattta taaatatcat tcactcatga tacccagctc cgtatcacaa ataatgatat
     9601 tttttaagag aaaaaattat ccctatttta aataatagca ataggtagtg gtagagagaa
     9661 atttataaat aactttgtta tttgctttgg tagccaagta agttgcaaaa gaaaaataga
     9721 tactgttaaa aagataatgt agacatagaa acaaatacgt aaaattttag ttagaatgat
     9781 ggcaaggaga aaaaacatga tagatgaaaa aaagtgttta aaaaatctat tggaagatat
     9841 ggatgatatt gaagaaaaac tatctaaatg gactgggaaa acaaatatat ttcagaa
//
LOCUS       DASHWH010000089         9792 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.092, whole genome shotgun sequence.
ACCESSION   DASHWH010000089 DASHWH010000000
VERSION     DASHWH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.092"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..3419
                     /locus_tag="U2R41_002455"
     CDS             141..3419
                     /locus_tag="U2R41_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEM8889809.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3960..5867)
                     /locus_tag="U2R41_002456"
     CDS             complement(3960..5867)
                     /locus_tag="U2R41_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEM8889810.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5997..6872
                     /locus_tag="U2R41_002457"
     CDS             5997..6872
                     /locus_tag="U2R41_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEM8889811.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6952..7926
                     /locus_tag="U2R41_002458"
     CDS             6952..7926
                     /locus_tag="U2R41_002458"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM8889812.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8106..9251
                     /locus_tag="U2R41_002459"
     CDS             8106..9251
                     /locus_tag="U2R41_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8889813.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9388..9732
                     /locus_tag="U2R41_002460"
     CDS             9388..9732
                     /locus_tag="U2R41_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEM8889814.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3192 a         1804 c         2058 g         2738 t
ORIGIN      
        1 tctttattat caacaactta agttatattt tttcttttta taaaatacac ttattataat
       61 tttgtttttt attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat
      121 ttgttacggg aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga
      181 atctccttat gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac
      241 aacagtctgt gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg
      301 aaataccaag aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag
      361 aatcagaaaa tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg
      421 ttgttttcgg agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg
      481 tgcggctctc ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga
      541 cctattatta tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa
      601 tagaatcttg gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag
      661 taccaaaaaa tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca
      721 gtcggatcga atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca
      781 aatatacgat gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc
      841 cgcaagctgc gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag
      901 cagggttctt ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc
      961 tgtttccggc cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg
     1021 gtttagaggc atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt
     1081 atgactggga aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag
     1141 caaggaatat gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag
     1201 atcctcctgg gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta
     1261 gtaatcattc aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat
     1321 tgacaattaa aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca
     1381 ctgcgaatcg ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt
     1441 tcagttttac gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg
     1501 aaacaaaaga gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg
     1561 taacaccaga agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac
     1621 aagcattgag atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct
     1681 tcgaaagagg attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt
     1741 tgattgcaga gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa
     1801 atgggatgac actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg
     1861 cagctatgca aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg
     1921 cttatcggaa tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag
     1981 catggcccgc gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat
     2041 ttgaagatgc cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg
     2101 gttgtatcgc gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag
     2161 gttcgatcgg aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag
     2221 gaattgaggg agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc
     2281 ctcatgaatt gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata
     2341 gcggaacaga tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg
     2401 ctctttatac ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc
     2461 gaacagagaa tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa
     2521 tctaccactc gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg
     2581 gacataccat atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc
     2641 agcctgttac gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac
     2701 cggggaatcg tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta
     2761 tttttgacgg agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac
     2821 caaatcagac aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg
     2881 tggcggagaa tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa
     2941 ctttcacacc aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga
     3001 tccaaatcgt tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag
     3061 caccttcaaa aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg
     3121 aaaaatggtc tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa
     3181 ctgccgatgt gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac
     3241 caaatggaaa accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa
     3301 aaaatggcac tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag
     3361 ctggaggaat tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag
     3421 tggaatttct ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa
     3481 tgagaccaat agatgtctca cttccttcta tctttttata gatgaggttc tccttgctgc
     3541 ttttgattgg ctgttcttat tacttgatgc aggacagctt tttcacaatc tctccactaa
     3601 acggtgttca atcagctgac cctcggtatt agctcaacaa acggcaaaat atgaggaact
     3661 atttttccgt ttttttctct aatactcagg tcaggacgct gatttcacaa cctctcgata
     3721 aacggtgttc aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaag
     3781 ccattttcac aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat
     3841 acatggtatt ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag
     3901 aagcgatttt cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct
     3961 taccagattc gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca
     4021 aatgtttcat agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg
     4081 gcatggacat cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa
     4141 atcgttgccc aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct
     4201 tccaatgcac agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg
     4261 tttacttttc cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg
     4321 ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca
     4381 aataaagcgc cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt
     4441 ccaccataat tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct
     4501 gccggaaaga cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc
     4561 atttcccatt ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg
     4621 agtttgctga aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt
     4681 ttgaactggt cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta
     4741 tttaatttta tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga
     4801 cgttttttat aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct
     4861 tcgccaaaat atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca
     4921 aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa
     4981 tagattccgc tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt
     5041 ttaatgatca tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa
     5101 tgatcgaagt acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc
     5161 gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc
     5221 ggattataca ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc
     5281 aatttatcaa attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg
     5341 gcaagctcta ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg
     5401 ggatggtctg atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag
     5461 aaggcgttcg tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt
     5521 aatgtatcca acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat
     5581 tcaacttttt cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa
     5641 tcgtttgcca gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct
     5701 ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa
     5761 tcttggaaac cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct
     5821 tcattgactg cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc
     5881 cttttttgtt tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa
     5941 aaaattgaaa attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga
     6001 aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag
     6061 ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc
     6121 tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc
     6181 cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga
     6241 agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc
     6301 ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc
     6361 cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta
     6421 cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc
     6481 caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta
     6541 gccagatcca accactagga cttcctacga ttccttttca ggttagtaca gggatttcta
     6601 gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc
     6661 agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac
     6721 ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag
     6781 gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca
     6841 gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt
     6901 ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat
     6961 aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa
     7021 aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat
     7081 ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa
     7141 tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg
     7201 aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa
     7261 gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa
     7321 gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc
     7381 tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa
     7441 ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt
     7501 gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat
     7561 gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta
     7621 gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac
     7681 ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt
     7741 attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa
     7801 aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc
     7861 aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc
     7921 gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg
     7981 cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa
     8041 atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg
     8101 aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc
     8161 cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca
     8221 tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag
     8281 acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata
     8341 atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta
     8401 cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg
     8461 acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag
     8521 cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt
     8581 tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag
     8641 atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat
     8701 taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag
     8761 acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag
     8821 gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg
     8881 aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat
     8941 tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg
     9001 tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta
     9061 atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg
     9121 tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct
     9181 ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg
     9241 tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat
     9301 tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga
     9361 gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc
     9421 gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga
     9481 tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa
     9541 agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg
     9601 tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa
     9661 atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa
     9721 aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga
     9781 tgactaactt tg
//
LOCUS       DASHWH010000090         9487 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.093, whole genome shotgun sequence.
ACCESSION   DASHWH010000090 DASHWH010000000
VERSION     DASHWH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.093"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..709)
                     /locus_tag="U2R41_002461"
     CDS             complement(<1..709)
                     /locus_tag="U2R41_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8889815.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPII"
     gene            complement(775..2064)
                     /locus_tag="U2R41_002462"
     CDS             complement(775..2064)
                     /locus_tag="U2R41_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEM8889816.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2151..2855)
                     /locus_tag="U2R41_002463"
     CDS             complement(2151..2855)
                     /locus_tag="U2R41_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEM8889817.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3055..3321
                     /locus_tag="U2R41_002464"
     CDS             3055..3321
                     /locus_tag="U2R41_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889818.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            3334..3492
                     /locus_tag="U2R41_002465"
     CDS             3334..3492
                     /locus_tag="U2R41_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889819.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(3489..3719)
                     /locus_tag="U2R41_002466"
     CDS             complement(3489..3719)
                     /locus_tag="U2R41_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM8889820.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3791..3946
                     /locus_tag="U2R41_002467"
     CDS             3791..3946
                     /locus_tag="U2R41_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889821.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            4103..4258
                     /locus_tag="U2R41_002468"
     CDS             4103..4258
                     /locus_tag="U2R41_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889822.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(4255..4479)
                     /locus_tag="U2R41_002469"
     CDS             complement(4255..4479)
                     /locus_tag="U2R41_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM8889823.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            4746..5081
                     /locus_tag="U2R41_002470"
     CDS             4746..5081
                     /locus_tag="U2R41_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889824.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5314..6255
                     /locus_tag="U2R41_002471"
     CDS             5314..6255
                     /locus_tag="U2R41_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEM8889825.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            6257..7147
                     /locus_tag="U2R41_002472"
     CDS             6257..7147
                     /locus_tag="U2R41_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEM8889826.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7190..7990
                     /locus_tag="U2R41_002473"
     CDS             7190..7990
                     /locus_tag="U2R41_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM8889827.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            8005..8856
                     /locus_tag="U2R41_002474"
     CDS             8005..8856
                     /locus_tag="U2R41_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8889828.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            8853..9212
                     /locus_tag="U2R41_002475"
     CDS             8853..9212
                     /locus_tag="U2R41_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEM8889829.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            9227..9388
                     /locus_tag="U2R41_002476"
     CDS             9227..9388
                     /locus_tag="U2R41_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM8889830.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         3221 a         1575 c         1817 g         2874 t
ORIGIN      
        1 caataatagg aggacagtaa tttttattat ttcttaattc accaatgtat tttttgttcg
       61 tcaacatatt tttaacactt tgataatctc taacgattcc atactgagag cctaaataat
      121 aaacagtttt tcttaaacta ccactagaaa gaaagtagtt gaatatatct atcacgatag
      181 gagcatcatc atttattact aatttcttat tttctataga gtagcctaac ggagttttcc
      241 cgcttactac ttcaccttgt gcaattttat ttgccataac agctttgatg cgttctgaat
      301 caatttgggc ttccaattca gcaaaactca ttacttgtgc aatgaaagtt ctgccatacg
      361 ctgtagttgt atcataatat tgttgagaaa cagcattcca agaaacattg tgtttttcta
      421 aaatttcttg agtgtttaaa taatgtcgta gatttctaaa ccatctatct aattttgtaa
      481 ataaaattag atctatttta ttttctttta catcttgtaa tagtttttga aattcatcac
      541 gatttatttt ttgaccagaa ataccaccgt ctacatattc attaactact ttcaaatcct
      601 tgtgttgttc gatatattca tagagggttt ctttttgttc tctaagacta tctccatgtt
      661 tggcttgttg ttcagttgat actctcatat ataatgctac tcgtttcatg ttatctcctt
      721 tcgaatgtat gttcttatcg ttgtaaaaga aaagcccgaa ggctaatctt tttactacac
      781 atctgttaat tctaatgatc ttctgtattg ttcagcttga gctagtttag taaaagatac
      841 agttccgtta taattttcta aaacaacttt ttcaatttca tcaagagata ctttaaagaa
      901 ttcttttcgt tgattaactt tattgacttc gttttttcta aaatgcgaat gtagttgatt
      961 ttctaatgtt ggtgcatctt ctgaaaatat cattgcatga acatcaaatt caaaaggtac
     1021 agaagcgcta ctaagttcct taattctatc cataggttct aaccgtctag tcattcctat
     1081 tttatagaca tcttcaccga aagatccgat attactgatg acataaacat agccagctct
     1141 agtattaagt tctctttgca atacatcttc tttgacagtt tctagttctc ttaatttctc
     1201 ttccagttct ttaattttat cagcatagag ttctttttca acatcagcac tagctttttg
     1261 catgtatgcc attaacttag ttatctctga gttaaactgt cgttcttctt tatcaatgcg
     1321 tttcttttca cgttctattt cacgtcttac cttttcttct tcaatcattt gttcttttat
     1381 tgctttttgc tgttcttttt catcgttaac tttaactatg tatgaatagt tgagttctaa
     1441 ttcttgaagt tttaattcaa gtaattcttt tttgaattct gcttcatcgt ttttaaaaag
     1501 cttgtttatt ttatcaaaag ttcttataat tttttctcta gttgaatcta cgttagaaat
     1561 agtaagttta ttgagaagtc cagttatttc tgattctaat ggtataagca ttttcttttt
     1621 aatattatta gcttctctat tattcaaata agaaggaata ttaattgcac cgtctaaaat
     1681 aaaatccttt tctttaatct ttaataaaga aagtttgttt tttatttcgg atgaagaagt
     1741 ttcatctata ttcggaataa ttactgattt atagatagtg accagagatg tgtcatattt
     1801 ttgattaatt tcatcgtcta accgctggct ttcagcatta agaatatcaa ttttgttttc
     1861 tagcgttgac aagtttttat tcaatttact tatgtttttc tctttttcag atataaacgt
     1921 ttccctagca tttaattctt gagaatattc ctttacctta ctctctaagg cagaagtttt
     1981 atcttttaat tttttagctt cagataattt gctactaccc ataattatta aaactattcc
     2041 aactaaaaac aatagtactc ccattcaaaa cgcctcgctt cttttataaa ataattgtca
     2101 aaatcatata aacccttacc gaagttgacg gctccggcat gggaattttt ttaaaataca
     2161 acttttccga taattctaat ctgttctggc gatacaattc tatcttcgta taagtcgttt
     2221 atagaacgta aaatgattgt ttgattttcg taatctttat atatctcttt acatgtaact
     2281 ccgtcaccac ctaattccac aatagcaatt tccccatctt ctacgtccgg ttgttcttta
     2341 acgaatacga attgtccatt cctgataaca ggttccattg aatcgcctat aataggtact
     2401 gcgaagtcag cagcacttgg aactctctca aattcttcct cttcgacaac cgtatcaccg
     2461 taagtaagtt ctgttggatt ggcagccgaa gccgaattgt gaacagttgg gattttttct
     2521 tttttaggaa aatctaggac attttcttgt ttttgttctt ctaatcgatt acttgcaaat
     2581 tcataggtag caactttatt ttcgtcgtta agctgattat atataggaag gatattatat
     2641 atctgattag atatagataa atcaatattc atcaaatcat ctatatcaac attgaatatc
     2701 ttagctatct catttaaggt ttttaatttt ggtgtatatt ttcctttttc ccattcgctt
     2761 attgaggatg aactttttct tcctagtttt tcggctagtt cttgttgatc aatattatac
     2821 ttttctctaa gaaactttag attttcagcg aacatagtaa cacctccttg agttaattgt
     2881 aacaccgttt cggaaaaaat gtaacaggtt tcttagaaaa tattttagtg tttttaaaga
     2941 agtatttacg caaaatagaa atcgatttca ggaaaaccga aatatgcact tgacttcgga
     3001 aaaaccgaag tatataattt agtcagaaag ttaaagagag gaggcgaata aaatatggat
     3061 accgcgaaga aagaaaaata tacattgaga caattaagag ctttaaaggg ttattcaaaa
     3121 gaagaactgt caaggaaaag caaagtgaca tcaagaacaa tatttattta cgaaaacgat
     3181 gtagataaaa tgaggaacgg aaagtacgcc actctagaaa aaattgctaa ggcattgggt
     3241 gttagagttt ccgatatttt tttagaccct gattcggaaa aaccgaaatc aataacaggt
     3301 gaaacaaaac aagtcagcta ggaggtgtga atagtggaag agaagaatat aaaagtaccg
     3361 actcttgaag aatatacaaa aattgttgtc gaaacagacg agcagaaccc gaaaacaatc
     3421 gcagttatta cggcgaatga tattgaatca tgcgaaggtt ttcgagttcg aatgacccct
     3481 agatatgatt aatgttcttt atcacgtgga ggtataggat cgtttccaaa actatctttt
     3541 gattgtattt gtccattctt tttttggaca ataaactctg attgctgttt tttagaaatc
     3601 tttttaccat actcaacagc atctgattta ttatcaaata gttttgtatc ttcggaatta
     3661 ccttcaccac gtacagccca tttgccattt ctaggagata cccattgatt tttacccata
     3721 acaattcacc tcgctttcaa gttaattcta ttaagaggtg aaaagaaaac aaactgatgg
     3781 aggtgagatt gtgagtaaca agccaaaaat cactttgaat ggcacaccta atcttcaaag
     3841 attagcagat gaaatggcta gaatacgttc tagacattgg ggagttactg tggtggcaac
     3901 cataattgaa caaaaagaaa agcccacaca gccggcaagc aagtagtggg caccatagaa
     3961 caataatatt atatcacaca ctcgccactt ccgaaaagag gagttgctgc tctcctttca
     4021 agtttgccaa agtccacgtt tcttcatttt ggcaactcct cttcttggag gtggtgacgg
     4081 cgagagacga aggagggatg acatgaagga agaatcaaaa atagggattt tgaatgagaa
     4141 caattacaca agattaatta ttgaaacaga agaaggggaa aaagtagcag aagttacatt
     4201 gaccgatgca actcctgctg acggttatcg tattcgatta actcctaaat atgattaacc
     4261 ttttggtggg tgtggatcgt ttccgggact atcacggctt tgaatttttc catctctgcc
     4321 atgaataaat agctcagatc cttgattttt agaaatctct ctagcgattt ttactgcttc
     4381 tgattttgta ttagtatgtg cagtcgcttt agaatttcca gcacctttga cattccaacc
     4441 accattggaa ttaggtacaa catgttgatt ttttcccata gttttcacct ccatttctat
     4501 agaaatattt tatcaaatag aaatggagaa attcaataaa cgaaaggagg gatcaaatga
     4561 cattacaaga gcatcaacac ttgatgcgaa aactaaatca ggaataccac gaggaaatga
     4621 aacctagatt gctaggggaa caatggcgtg aacgccagaa gaagtggtta gaaataaaaa
     4681 agtgactcag ccgtccaaag caatgagtca caaaccaaaa attataccta aggagatttt
     4741 atcacatgaa taagaaaatt gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc
     4801 aaggtgtttc tattatttgt acagcccaaa aagaagggga acttaaaagt ctcgtttatg
     4861 gtgagacaac agaaatttta ctttgtcttg ctatgcaaga agaacattta gatgaaaatc
     4921 tcccattgtc agcacatatt atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa
     4981 atgaagaaga aaatcaacct tcaaatcata cttttgttat taataacaag gaagatttag
     5041 ctgatgtgat gactcgtatt ctcaaggggg aatttcaatg atgaatgaaa aaacaattga
     5101 tctactcatt attttatttt tcacgttagt gattgtagcg aaaagttatg taatctttgg
     5161 cttgttattc gcaattacca tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa
     5221 acatgaacga gttagcaaat cttgacaact atttaactga tcctgattat gctaaaccac
     5281 catatgaagc gccaattgat gaggaggatg aagatgagta aatctacctt agaaatgagc
     5341 catcaagaat ggctcgaaga ccgcaaaaga ggtattggtg gctcagatgt tgcaaccgta
     5401 cttggattaa acaaatacaa atctccttat caattgtggc ttgagaaaac gggtcaaatt
     5461 gaattgaaag atttagaaag cgaaccagct tattggggta atgttttaga agaagtggtt
     5521 gctaaagagt ttcaggaacg tacaggcaaa aaagtacgca gaagaaacca agtatttgaa
     5581 catccattac atccattttt aagagcaaat attgatcggg aagtagtggg agaaaatgcc
     5641 attctggaat gtaaaacagc caatcaattt ctcggaaaag agtgggaagg tgaagaggta
     5701 ccgcttagct atctctgtca agttcagcat tacatgaacg ttttaaacaa agactattgt
     5761 tatatcgctg ttttgatcgg tggacaaaaa ttcatttgga agcggattga acgagatcaa
     5821 gagctgatcg atacaatcac tgaacaatta gtagagtttt gggaaacgaa cgttcttgga
     5881 ggtatcgagc ctgtaattga cggaagtgga gcgactgctg acttcttaaa agaaaaatat
     5941 gcagatgtag aagaaaatca aacagctcta ccatcacgtt ttgatgaact tatcgagcaa
     6001 aaaagagaac tcaaacggac gaaaaaagaa attgaatcag ctatccgtca agtagacaac
     6061 gagattatca gcgagctagg taaacgtgaa gccagtatcg gtatcactca aaaaaacatc
     6121 atcagttgga aatttgtccg tacgagacgt atgaactcga agaaactagc agagaaatat
     6181 ccagatgtcg caaatgatga agagatttat aacgttactg aatcaagaag gctaaccgaa
     6241 aaggggatca aataatatgg caacaaatga atcgttaaaa aatcaattgg cagaaaagcc
     6301 acagaaacaa gttgcaccag gacagttagg gcttaaagct ctaatgaata caccaacaat
     6361 gagaaagaaa tttgaagaag tacttcatga caatgctaat gcttttatgt cgaatgttat
     6421 gactcttgta tctaatgaca gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg
     6481 tgcgttaact gctgcaacat taaatcttgg gctagataag aatttaggtt atgcatattt
     6541 agttccattc aatagtaaaa acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat
     6601 gcttggctat aaaggatata tccaattagc ccaacgatca ggtaaataca aagcattaaa
     6661 tgtgatcgaa gtttacgaag gagaactaaa aagctggaac cgactgacag aagagtttga
     6721 gtttgatcca aatggtagaa cgtctgatga agtcattgga tatgttggct atttcgagtt
     6781 actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat
     6841 tgctaacaat aaagatcgcg ataagacaaa gttaagtggt gtgtgggcat ctgattacaa
     6901 tgcaatggca cgaaaaactg ttttgagaaa tcttctttct aaatggggaa tcttgtccat
     6961 cgaaatgcaa gaagccacca catcggatga gagagtccaa agggttcaag aagacggcag
     7021 cattattgct gaaacagaag ttgaagaaga tattcctgaa agaaaagaag cagaggttat
     7081 atctgaagaa aatgaagatg tacaaactgg attatttgat gcatctaatc cgccgttaaa
     7141 caaataatga gggagctttc tcccttgctt ttctagagga gaaatacgaa tgaacacagg
     7201 atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt tggaccaatc cttatatgta
     7261 taaattatgg agcttgtgtt tgatgaaagc gagtcatgag aatcgcaaga tactttttaa
     7321 tggaaaagag atacaagtga acagcggaga attcgtcaca gggcgcgatg ctatcacatc
     7381 tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac agcggttctg tatggagatg
     7441 gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa tcaactacga aatacagcgt
     7501 tatatcaata aaaaactggt ctttgtacca aagcagtgaa caacaaatga acatcaaacg
     7561 cacaacaagt gaacaacaag tgcacacaaa caagaatgaa aagaattata agaatgaaaa
     7621 gaataataaa acatcatcgt cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt
     7681 ttactacatt ctcgcggagg agttattcaa acagatttgt cagaatcagg aaatcaaaaa
     7741 gccgaatctg caaagttggg cagataacat tcggaaaatg atcgagatcg acaagcggac
     7801 cgaaaaccag gtacggggaa tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa
     7861 tattttatct gctaaaaaat tgcgagaaaa atacgacaca atggcagcgc aagcaaatcg
     7921 tgattataaa acaaaacaaa ctaaaacgct tgaatacgag aaatttagca cagatgagtt
     7981 gcctatttga gaggaggcgt aggcatggaa actgttgggg aaatcatgga aaagctgata
     8041 cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg gacagccttt gtatggatgg
     8101 cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat
     8161 aaagcattgc gagttcagga agacttacaa acagaacgaa tttacaacga gagcctgaaa
     8221 gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat
     8281 tgcacattgc agaattatca aattgtcgat caagaaacaa gacaagcggt tgaagtaacc
     8341 aaacgctttg ttaattcagt cttgttagga aatccaagtc accttgtttt aactggaaaa
     8401 caaggaacag gtaaaagtca cctagcgatg gcagcggctt gggaagtctt gaagcgatca
     8461 aactacgata aaaaaatctt atttatcggg ttacaggaaa tgctggatca aatcaaattt
     8521 tcgtacaaca atcctgaact cagaaaaacg attgagggat cgttgattgc agatatcaaa
     8581 acagcggatt tagtcatcat tgatgatatc ggttcagaac taggaaaaga tgcatcagat
     8641 agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg atgcacgaca gaacctagca
     8701 acgattatca caacgaactt gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca
     8761 atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg tattttctca aacagcagac
     8821 aagcgcataa aaccagtgaa aggtagtatc gcatgaataa ataccgtaat aaaaaaactg
     8881 ttcatcgagg tatcaagttt gattctatcg cggaagcaga gtattacgat ctagccttgt
     8941 ggcaagctga agcgaatggt tggaaagtga agctccagga aaggtttgag ctgatgccga
     9001 agtttgaact agagggcaag aagtatcgca agatcgagta tattcccgac ttcacatttt
     9061 acaaaagcgg caagctggtc aaagtcgtag atgttaaggg gatgcagaca aaagacttta
     9121 agatcaaggc aaagttgttc tgtcatcaat atcaagtgcc gttgatatta gccaaaaaat
     9181 atcggaatac gttcaaggaa gagcgttttt aacgaggtgg tccatcatga caacagaaga
     9241 agtgattcaa atgcgaattc gaagccttcg gcgtgagatt gacgatctgg aacgaacaaa
     9301 ggcagtgatg gtcaatgaaa cggctagaaa ggcaatcgat ttgcacatag agaatttaag
     9361 aagggaaatc cgtagattgg aggaatgagc gtggataagg aagcggcttg gcgaaaatta
     9421 atgttgctga ttcaagatga gaactggcaa gaagatgaag cagtggttgc tgaagttcag
     9481 cgtctag
//
LOCUS       DASHWH010000091         9316 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.094, whole genome shotgun sequence.
ACCESSION   DASHWH010000091 DASHWH010000000
VERSION     DASHWH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.094"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..2122
                     /locus_tag="U2R41_002477"
     CDS             <1..2122
                     /locus_tag="U2R41_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889831.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="U2R41_002478"
     CDS             2119..2739
                     /locus_tag="U2R41_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8889832.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="U2R41_002479"
     CDS             2853..2990
                     /locus_tag="U2R41_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889833.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="U2R41_002480"
     CDS             3432..3812
                     /locus_tag="U2R41_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEM8889834.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="U2R41_002481"
     CDS             3794..4708
                     /locus_tag="U2R41_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM8889835.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="U2R41_002482"
     CDS             4948..5637
                     /locus_tag="U2R41_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889836.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5757..5843)
                     /locus_tag="U2R41_002483"
     CDS             complement(5757..5843)
                     /locus_tag="U2R41_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEM8889837.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6488..7225
                     /locus_tag="U2R41_002484"
     CDS             6488..7225
                     /locus_tag="U2R41_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM8889838.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7253..7792
                     /locus_tag="U2R41_002485"
     CDS             7253..7792
                     /locus_tag="U2R41_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM8889839.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7808..7978
                     /locus_tag="U2R41_002486"
     CDS             7808..7978
                     /locus_tag="U2R41_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889840.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8290..8751)
                     /locus_tag="U2R41_002487"
     CDS             complement(8290..8751)
                     /locus_tag="U2R41_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8889841.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8884..9258
                     /locus_tag="U2R41_002488"
     CDS             8884..9258
                     /locus_tag="U2R41_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEM8889842.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3596 a         1414 c         1455 g         2851 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt tcaggttgat gtcgccttta ttgattttac
     5761 ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaactcc tacaagaagg
     5821 gggcaaatga acagttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata
     5881 ggcgacaagt tattatatct taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg
     5941 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gcttctcgac tagattaagg
     6001 gaaattaatc aagaattgtc ttagaacggc ttagacaagc tttcagagtc tcacacctcc
     6061 tctttgaaca acggttttag caaaggagcc ggaggcgact gcgcagtata atatgagagc
     6121 atagcgatca caccttttta gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg
     6181 cgaacctttt tggaggaaaa agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa
     6241 gatatactgt tagatatact gttagatata ctgttaataa taactgttag ggattagcaa
     6301 ccttatggga gagtaagtat tgacataggt agtgggggat aaattgtcac actatggggg
     6361 ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat tgtcacacta
     6421 tgggggataa attgtgtttt acatcctctt atgtctatga taacataatt tattgaggtg
     6481 atttttattg agtaacgata tagttcgata taataatgga ttaaattctg tccctttaag
     6541 aaaatttacc cctatagata tggatttatt ttggggaata tgttctaaaa tgaaaagaaa
     6601 aggtacaaaa gaagaaattt ttacatttaa acaaataaaa gaattaattg attacaatat
     6661 aaatgcacgt ggcgaccgtt ttgctaatga tttaataaaa atgtcaaaaa aaatcattac
     6721 tttgtctttt atttatgaag acgaaaaaac atatgaacaa ttaaatctgt ttcaaaaatt
     6781 taaaatcgat aaagtgaatg aaacattatt aataaaggtt tctgaagaat ttgaatatat
     6841 acttaattca atcggaacta attttactcg tttcgaatta gaaaatatga caaacttaaa
     6901 gagtagctac gttaaagagt tatatcgcca acttatggct cataaagaca gaagatctaa
     6961 aaaaggtcac tggtttgtaa aaatagaaga ttttagagct ctattagcaa taccagaaag
     7021 ttatcgcatg agagatattg ataaacaaat ttttgcccaa gcgaaaaaag aatttttaaa
     7081 cgttaattca actcaccctg ctatttttaa aacttttgaa atcaaaaaaa ttaaagccta
     7141 caaaggaaat aagataggat ctctgcaaat ttattttgaa gaaaatatac cttcaattac
     7201 cttagataat tggttagaga aataatacct aacaatttag gaggcaatta tcatgcaacc
     7261 caaagaaacc tttacaataa aggaattatc tgaattattt gaaataagta ggcaagcaat
     7321 ctctaaacat gttgataaat tagacccgtc cttattagct aaaaatgagc gtggttataa
     7381 ggttgtatta cgttctggag tattgcaact tgctagcaac ttaggtaacc aaaggttact
     7441 atataggttg catgagtatg aacaagaaaa cgataaaaac gtttatatat caagcaaaaa
     7501 cgtgattgta aacgagttgc tagataagtt gcgtgaagaa aatgaatttt tacgagaaca
     7561 actttccctc aaagagcaaa ataattctaa tatgcaaaaa ttactcgacc agcaacaaca
     7621 gctcacccta caggcaaatc aacagatcga aaaactgcaa gaacagttac aattagctta
     7681 tacggaagaa actccagaag atgaatcaac tactattctt tctgaagaaa cagaatcgat
     7741 agaaaaacac acaaaaaaga agaagcaaaa atggtggcaa ctttggaaat aactggaggg
     7801 aacattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa ttctaggctt
     7861 cagtccgatg ttattgtcag taatcaataa agggaataaa catggtgaat tgaaattgaa
     7921 tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata
     7981 aaaaagactg ctcttccgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag
     8041 cacgcgctta tgaagcgcta ccaaaaattt accatatttt acaaaaaata acaagtctta
     8101 aaaaaatgac agataaaaat gcacaaagaa ttagatatag ataaccgcga aaaatcaagc
     8161 tttagaaagt tgcttttagc tagaccaatt aaaaatcaca aagtgagcgt ctctatccat
     8221 tggtagaggg gctacttggc atagtgtaca ttatgtcaac tatgcaatta aataactaac
     8281 tacttcatat tagcttaata aaataatcaa aataactagg gggaaaaaca ttgtataaaa
     8341 aaatttttca ttttttaaat aagaatagta aatataaaaa taatttaaga tatctattac
     8401 tattaatcca gatagagcaa accaagtaaa tattccatgt gtaaagctaa aaaaattcat
     8461 ttgaatcaat tcaaataaac ctaatatagt aacaaatata ttaaaaataa tagctgtcaa
     8521 taccagtaaa ttattttttt tattttttga attatcttca tttaaaatag aatttatgtt
     8581 agtactataa aattcacaaa ttttttttag tgtctctata tctggtgttg ttttgttatt
     8641 ttcccacttt gaaatagttt gacgtgtaac gttaaatttt cttgccacat attcttgagt
     8701 ataccctttt ttcactctta attctcttag cttagatcct aaatcctcca ttttttctcc
     8761 tatgtaaacg attatttaca taaaatttta tcataaaaat actaacgcgc caaaaaagtt
     8821 ctttatttta taaaacacac taaaataaaa taattctagt aaaaaagata ggagaaataa
     8881 gttatgaaaa aatttttatg tctttctgct ttaatgggtg tcttattaac tagtggagga
     8941 atagctagtg ccacagaagc tttaaatcta gatgtacccg aagaccatac cgccatttac
     9001 ggaggaggaa tggaagctac agaagaagga ttttcttcta gtaaattgag atatgctgct
     9061 ggcggcgggg atttcaactg tggggttaat ggatttaaag tttatgcaaa ttactaccat
     9121 gctagagcta aacatagtgc aacagctaaa aatggacgag gcggtcaagt acgatcagtt
     9181 cagaaagctg gagtaagagc ttatgcaaca tgcaatgcca cacttactgg aaatactggt
     9241 tggtggaatg tttattaaaa aagagagttt aaactctctt ttttatttac atttgaaagg
     9301 aaattcaaaa tatgtt
//
LOCUS       DASHWH010000092         9154 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.095, whole genome shotgun sequence.
ACCESSION   DASHWH010000092 DASHWH010000000
VERSION     DASHWH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.095"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..267
                     /locus_tag="U2R41_002489"
                     /pseudo
     CDS             1..267
                     /locus_tag="U2R41_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            806..1006
                     /locus_tag="U2R41_002490"
                     /pseudo
     CDS             806..1006
                     /locus_tag="U2R41_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="U2R41_002491"
     CDS             1493..1672
                     /locus_tag="U2R41_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889843.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="U2R41_002492"
     CDS             complement(1718..2323)
                     /locus_tag="U2R41_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8889844.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="U2R41_002493"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="U2R41_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3005..3232
                     /locus_tag="U2R41_002494"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="U2R41_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="U2R41_002495"
     CDS             3358..4329
                     /locus_tag="U2R41_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8889845.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="U2R41_002496"
     CDS             4469..5782
                     /locus_tag="U2R41_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM8889846.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="U2R41_002497"
     CDS             5825..6691
                     /locus_tag="U2R41_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8889847.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="U2R41_002498"
     CDS             6688..7536
                     /locus_tag="U2R41_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8889848.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="U2R41_002499"
     CDS             7550..9022
                     /locus_tag="U2R41_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEM8889849.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9154
                     /locus_tag="U2R41_002500"
     CDS             9039..>9154
                     /locus_tag="U2R41_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8889850.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1525 c         1871 g         2838 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caat
//
LOCUS       DASHWH010000093         9013 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.096, whole genome shotgun sequence.
ACCESSION   DASHWH010000093 DASHWH010000000
VERSION     DASHWH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.096"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(548..910)
                     /locus_tag="U2R41_002501"
     CDS             complement(548..910)
                     /locus_tag="U2R41_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889851.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(984..1364)
                     /locus_tag="U2R41_002502"
     CDS             complement(984..1364)
                     /locus_tag="U2R41_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEM8889852.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1428..2621
                     /locus_tag="U2R41_002503"
     CDS             1428..2621
                     /locus_tag="U2R41_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8889853.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2698..3282)
                     /locus_tag="U2R41_002504"
     CDS             complement(2698..3282)
                     /locus_tag="U2R41_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8889854.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3483..4481
                     /locus_tag="U2R41_002505"
     CDS             3483..4481
                     /locus_tag="U2R41_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8889855.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4593..5102
                     /locus_tag="U2R41_002506"
     CDS             4593..5102
                     /locus_tag="U2R41_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEM8889856.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5132..5791)
                     /locus_tag="U2R41_002507"
     CDS             complement(5132..5791)
                     /locus_tag="U2R41_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEM8889857.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5793..6557)
                     /locus_tag="U2R41_002508"
     CDS             complement(5793..6557)
                     /locus_tag="U2R41_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEM8889858.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6565..7242)
                     /locus_tag="U2R41_002509"
     CDS             complement(6565..7242)
                     /locus_tag="U2R41_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEM8889859.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7279..8664)
                     /locus_tag="U2R41_002510"
     CDS             complement(7279..8664)
                     /locus_tag="U2R41_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM8889860.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8874..8958)
                     /gene="ffs"
                     /locus_tag="U2R41_002511"
     ncRNA           complement(8874..8958)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U2R41_002511"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         2706 a         1766 c         1541 g         3000 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
      301 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      361 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      421 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      481 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      541 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      601 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      661 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      721 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      781 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      841 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      901 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      961 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
     1021 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
     1081 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
     1141 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
     1201 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1261 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1321 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1381 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1441 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1501 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1561 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1621 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1681 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1741 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1801 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1861 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1921 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1981 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     2041 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     2101 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     2161 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     2221 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2281 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2341 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2401 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2461 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2521 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2581 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2641 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2701 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2761 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2821 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2881 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2941 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     3001 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     3061 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     3121 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     3181 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3241 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3301 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3361 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3421 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3481 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3541 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3601 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3661 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3721 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3781 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3841 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3901 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3961 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     4021 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     4081 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     4141 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     4201 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4261 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4321 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4381 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4441 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4501 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4561 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4621 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4681 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4741 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4801 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4861 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4921 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4981 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     5041 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     5101 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     5161 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     5221 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5281 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5341 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5401 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5461 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5521 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5581 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5641 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5701 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5761 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5821 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5881 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5941 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     6001 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     6061 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     6121 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     6181 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6241 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6301 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6361 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6421 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6481 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6541 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6601 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6661 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6721 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6781 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6841 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6901 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6961 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     7021 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     7081 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     7141 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     7201 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7261 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7321 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7381 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7441 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7501 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7561 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7621 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7681 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7741 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7801 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7861 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7921 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7981 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     8041 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     8101 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     8161 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     8221 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8281 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8341 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8401 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8461 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8521 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8581 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8641 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8701 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8761 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8821 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8881 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8941 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     9001 agtctttttt taa
//
LOCUS       DASHWH010000094         8966 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.097, whole genome shotgun sequence.
ACCESSION   DASHWH010000094 DASHWH010000000
VERSION     DASHWH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.097"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(54..347)
                     /locus_tag="U2R41_002512"
     CDS             complement(54..347)
                     /locus_tag="U2R41_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEM8889861.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(375..1040)
                     /locus_tag="U2R41_002513"
     CDS             complement(375..1040)
                     /locus_tag="U2R41_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEM8889862.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1180..1812)
                     /locus_tag="U2R41_002514"
     CDS             complement(1180..1812)
                     /locus_tag="U2R41_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8889863.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1819..2886)
                     /locus_tag="U2R41_002515"
     CDS             complement(1819..2886)
                     /locus_tag="U2R41_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8889864.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2883..3614)
                     /locus_tag="U2R41_002516"
     CDS             complement(2883..3614)
                     /locus_tag="U2R41_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEM8889865.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3783..4262)
                     /gene="greA"
                     /locus_tag="U2R41_002517"
     CDS             complement(3783..4262)
                     /gene="greA"
                     /locus_tag="U2R41_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEM8889866.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="U2R41_002518"
     CDS             complement(4398..5573)
                     /gene="mltG"
                     /locus_tag="U2R41_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEM8889867.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5777..6946
                     /locus_tag="U2R41_002519"
     CDS             5777..6946
                     /locus_tag="U2R41_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM8889868.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7213..8847)
                     /locus_tag="U2R41_002520"
     CDS             complement(7213..8847)
                     /locus_tag="U2R41_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889869.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2854 a         1701 c         1333 g         3078 t
ORIGIN      
        1 attttcaaaa gaaaaaagag aaggtgacaa aaattgtgtc accctctctt tttttattgt
       61 tttttaaagt gataggttgg ctcctccagg ttgggatcat aatcaacaag taaatcatat
      121 cctttaaaga accattcatc tgcttcctcg ataaaaaaaa gtgtcccatc aatttccact
      181 ttctttacag gatgttctgg ctgttcaact gacattccaa tcgaaaaacc atcatgaacc
      241 tctgttttgc cgtagacttt tccaaaaaaa cgtattccgt aagtttctgg cagatcaatt
      301 tctgagtcaa accacttttg tgcttttggc gttaccgtta atttcatccc aacaacctac
      361 tttctctttt ctttctattg attcatctta tcgtctagaa tatccacatc gcctgtctct
      421 ccaataacca gtagattatc attttcacga acatattcat ctgctgcagg agaagcgata
      481 accgtttgat ctactcttcg tatggcgact actgttaagt caaaacgttg cctgaaattt
      541 aattcagcta atgttttgtt gaaaaattta ggattggaga ctcggatttc tgctaaagaa
      601 tatttatctg acaactctag gtaatctaaa atattctttg acactaaatt atgcgcaatc
      661 cttattccca tgtctcgctc gggatggacg acgcgatcag ctccgatctt ttcaagtact
      721 ctggcatgat actcattttg ggcttttgct aaaatatttg gtacgcccat ttcctttacc
      781 atcagagtaa ctaagatact ggcttgaata tcttctccaa tagcgacaat tacatgatcg
      841 aaatttctta tgcctaatga tcgaagtgtc atttcatcct gtgcatttgc cacaacagca
      901 tgagtggcta tattcatata ttcattgacc cgatcttcat tactatcaat agccagtact
      961 tcttgaccag cttcgatcag tgttcggcag atacttccgc caaatcgtcc aagacctaca
     1021 attgcaaaat tttgtttcac cgttttcctc ctttagtatc ttcttttctc agtataacaa
     1081 agcctttaca aaaacaaaag caagcatcat tattgtaaaa aagccaagaa aaataatagt
     1141 tattttctca gcttttgatt atcctcttta tcatcgtttt tatttcacca aaccatgttt
     1201 gaatgcatag atagcggctt gtgtacggtc ttccacttct agcttagcca atatattgga
     1261 aacatgtgtt tttactgttt tcagtgtaat aaacaattcg tccgctattt cctgattact
     1321 ttttccttca gaaatcagca ttaaaatttc attttcccga ttcgttagtt cttcatgtaa
     1381 tacaggctca tttcgccgac tcattttttc catcattttt gttgtcactt ctggttctaa
     1441 tactcgctcg ccacgttgcg ttgcccttat agcatcagca atttcatgtg cggtagaggt
     1501 cttcaacaaa taccctgctg cacctgcttc aatggcagga taaacttttt cgtcatcaat
     1561 aaaactggtt acgataatga ttttagcttc tggccaatct tttaagattg cttttgttga
     1621 ttcgatacca tccatttctt ccatgactaa gtccattaaa atgacgtccg gtcgaagtgt
     1681 catcgccttc tcatagcctt gtcgtccatt ttcagcttct ccaattactt caatgtcttc
     1741 ttgaatagat aaataagaag agacgcctaa gcgcaccatt tcatggtcat ctactaataa
     1801 aacttttatc atccttatct atgcctcctt catcaaaggg accttgattt ctacacttgt
     1861 accttgacct ttaaaactaa tgatttttac tgttccacca ataccagcta ctcgttcttt
     1921 gatattgttc aatccataac ttcccgtttt cgtttcattc atattaaacc ctgttccgtc
     1981 gtcaataata cgtaacaaaa gattattgtc tattttgtgc aaatatacct ctaattcatt
     2041 ggctttagca tgtcttaatg tatttgataa caattcttga acgattcgga acaagtgatc
     2101 ctcaatggaa ctagatagtt ttacatcttc tacatcccat ttcagcgaaa tctggatttt
     2161 attttgcaat tccttcaata actgctcgat accttgcttt aaacttttct cttctaaatt
     2221 gactggacgt agatgaagaa gcagcgcacg catctcagac tgggaagcgt tgatgatctc
     2281 tgctaccatt ttcaactgct tgcgaaacat ttctggtgtc tcgctttttt ctgcttgctc
     2341 cgtcaatgcc gacatcatca tcattgctgc aaacaattgt tgcgaaactg aatcatgcag
     2401 ctcacgggct aatcgatgtc tttctaattc taaaatttct tctttggttt gtccatctac
     2461 atattgggga cggctcgtta caatttgtaa ttcactagat acttcaatca ttttttcttt
     2521 tatcttagtg atttccttat caatatcttt gatgtataaa tcgtccgatg cacttgggat
     2581 cggaagaagc aacaatttgg attcataatt gccagaagaa agtgcacgca gcttttcttc
     2641 gattttcccg tattttgtct tttgaataag tgataataat agaaagacaa ttaatcctac
     2701 acctaaggaa atggctaaca aatggaagat aagcggaaca tataataaac gtgcacgcag
     2761 taattctccc caccaatgac tttgattgct ggcataaaaa taagtgaaaa gtgaaaatag
     2821 aataataagg aaagccgcaa tccccgaata aacagctagc atcgctctgg atatttttcc
     2881 catcatattc gtatcacctc gacatctcca atcaaggtat tagtaataat cttcaatcta
     2941 cgagggtttt catcataatc actgctgtag attttgattt gttcattttt caaagaaaat
     3001 tgctcttctt caaacaagac atttcctact aatgtcgcat gctctaatcg gatggctacg
     3061 cccaaaggta ctaagatccg tgttctgcct atcccttttc ttacaatcac aatattatca
     3121 tcttttggca aaagcgtatt gcccaaatct atgatagtat caccagaaat gacggcgatg
     3181 ttgatatcgt cccattcata gacgtcgttt ccaatacgtt ggttaccgaa taattgctgt
     3241 cttttccgct cattattatg tgtttttaat tgctctgttt ggatcatcat gatctgtttc
     3301 tttcgccaaa aagcattttt ggttaaatct atccctgaaa tctctacacc tttcagacca
     3361 ataaataaaa cggcaaatac agccatcatc cataaagcag gactgttgac taagctaaaa
     3421 aaaatgacca aacttcctaa tatcaattga aaattttgaa atttagtacg aggatgtttg
     3481 cgcatagcta gatatatatt gaaaataccg aatatcacta aaagcaaaag tccagtattg
     3541 tttacgattt gccaaacagc aaataacagc agtagtgctt caactaccac aaaaaaacgc
     3601 caagaactat tcatcgctct ttctccctcc tgacacattc tcatttcatc atacctattt
     3661 ttttaactgt tggctatagg actttagatg taagatcgtt tgttgaaatc taaaatacac
     3721 aaagagaaaa agatctagag tgtatcatca gtaagatgaa ctctctagat ctttcagtca
     3781 gactacgctt gttctacttt gacgatttta actaacatat cgccacctgg agtagcaata
     3841 gttacttcat catttttacg tttgccaatc aatgcttgcg caataggaga atcattggaa
     3901 attttgccag aaaacggatc tgcttcagcg cttccgacaa tcgtgtactc ttcttcttct
     3961 ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc tgcatctaca
     4021 ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt gattcttcct
     4081 tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga aagatctccg
     4141 aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt ttttagttct
     4201 tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac tttctctacc
     4261 atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt gaggaatata
     4321 gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa aatgatcaat
     4381 gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc catatgttct
     4441 tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa ataataatag
     4501 tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt gtcaaaaggt
     4561 ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc tgtatcttca
     4621 tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc tgactgaagt
     4681 ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg atcattttct
     4741 tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac agtcaaattt
     4801 ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt caccatttca
     4861 atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg gaataagtaa
     4921 ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag ttctgggaat
     4981 ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa ttctttcttg
     5041 tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta cccttctgga
     5101 atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc ttgcaactgt
     5161 ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc ttgaaagcca
     5221 gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat aatattgtcc
     5281 ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc gacttggact
     5341 aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg atacacggta
     5401 aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat gatcttcctc
     5461 acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc tgctgtagct
     5521 gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga caaaacaact
     5581 cctccttact atcaagttcg taactttatt cattatacat gatatttgaa ttggcgtgaa
     5641 tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact ataagattaa
     5701 aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa aaagaaagga
     5761 gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat attcattttt
     5821 aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta ttcttggatg
     5881 gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag ctcaaaaaaa
     5941 gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac tattaagaaa
     6001 aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg
     6061 ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt attatcagat
     6121 tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg agcgtgggca
     6181 cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca gccagcaaat
     6241 tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt ctccaaccat
     6301 accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat ttacagatta
     6361 tcagacctct cattatgatt cattgaactt taccatattg actggaattt tgatcaaatt
     6421 aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt taggattaca
     6481 gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat cctatcaaat
     6541 gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga tccataacga
     6601 attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt ttacacaagt
     6661 tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg aaaatgcgaa
     6721 tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt cgcaaggaaa
     6781 tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg ctatcatcat
     6841 ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc taagaaacca
     6901 aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg atgaaaagat
     6961 agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact aaacgtagga
     7021 aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc caaataaaca
     7081 gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat ttctctttac
     7141 caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat tgctaaaata
     7201 tgtgctagca aatcattcat ggcttgttct tatttttaat tttatgcaat aaaaaagaag
     7261 aacaattagc gtgattatag aaataaataa tgagatttta gaaaaaaatg tgtatgaata
     7321 cctgattgtt actttattta ttccactttc actttttact gtaacaaaag gacctccttg
     7381 ttttagtact gtagcatttt ttgaattaac agtaaccgtt aatcctttat accggaatac
     7441 aggtaaaact attgttttgc cttttgaaga tttaagcgta aatgtaattt catctttatt
     7501 tggagaaaag ctactataaa taacttgacc atcttctgta acttgatggt ttttcaaaag
     7561 caattgatct tttttatagc ctataggtac ataatcaaaa ttctcaaatt cttcaagttt
     7621 attttccatt tgttcattag aataagttcc ttgaaaattc atttctttca ccatgttaac
     7681 tactgatgaa taatttaaaa ttgaaatagc taatattaat aatagtataa aatttcttct
     7741 ccttgtataa gaaaattttt ccaactgtgg agaaataaat agagatactg aaaaagatat
     7801 gaacaatgtt gaaaatatca taaaacgcca aggagcttga ataatattta aaggagtatt
     7861 ttgtaaggca aaccatggaa atatttttgt acaaagtaga atagatatta tacccattat
     7921 ataagtgatt ttagataaga ttcctaattt tttaaagtta aatgttagta taattaaaca
     7981 aataagcaaa gttaagccaa taccaaatga tagtaattct gaatctatac ttccaatcaa
     8041 aaactcttta cccagataaa ttgctctttt ttgtaatata tccacataag gtctattcag
     8101 atcttgatac aatgtctgct caatcatagg acctataaac gctagtgaaa gaagtaaagt
     8161 aactatagca gcttttatta atttaataat taagaagatt tctattttct ttttccgata
     8221 aaattgaata attaaagcaa ttataatcat taccatagtc atagctacag aaagaacatg
     8281 agtgtaaatt aataaagtca tgctaatcgc caacataacc caagattctt tttttgagtc
     8341 gtaaagcttt ataaacccga gtaaaataag aggaagtatt gacattgcga gtaattcccc
     8401 cattgcccct cgaagaaata tttctacact cctaaaagaa gatgttgtat atataatagc
     8461 gaaaaaggag ctagtaacat gttttttggt aatttcatac attacataat gacatataaa
     8521 tagagtaatg agcgtaacaa tataaagaaa aaggttatag cctattgcta aattagtaaa
     8581 cttgatcaat aaaaataaag gatatagagt taaccatggg taaaaattat ttacttgcat
     8641 gccatatgat ctaaacgttt caaaattaat aggggagcta aaaatagatt ctaaacttaa
     8701 cgcacgatta atgtgaaatg ccgtatcata aaagccaaat atttccatgt ttttaaatgc
     8761 tggaaataaa tataataaac taacaaatgc ataaactata aatattttta cagaaaaaat
     8821 ttttttcaaa ttcatatacc acctcatgtt tttttatatt acatatagta tcatttattt
     8881 ttttaaaaca ctagattagt cactatataa aatgtgatta attttattat taataaaata
     8941 ttttttatat tacaaaagta gtgttt
//
LOCUS       DASHWH010000095         8693 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.098, whole genome shotgun sequence.
ACCESSION   DASHWH010000095 DASHWH010000000
VERSION     DASHWH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.098"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..400
                     /locus_tag="U2R41_002521"
     CDS             <1..400
                     /locus_tag="U2R41_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEM8889870.1"
                     /translation="ADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSI
                     TSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKLYRSLRTASTTIKGMEAI
                     RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(1016..2056)
                     /locus_tag="U2R41_002522"
     CDS             complement(1016..2056)
                     /locus_tag="U2R41_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8889871.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2405..2731)
                     /locus_tag="U2R41_002523"
     CDS             complement(2405..2731)
                     /locus_tag="U2R41_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889872.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2718..3521)
                     /locus_tag="U2R41_002524"
     CDS             complement(2718..3521)
                     /locus_tag="U2R41_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8889873.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3874..4164)
                     /locus_tag="U2R41_002525"
     CDS             complement(3874..4164)
                     /locus_tag="U2R41_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEM8889874.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4300..4479)
                     /locus_tag="U2R41_002526"
     CDS             complement(4300..4479)
                     /locus_tag="U2R41_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889875.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4476..4826)
                     /locus_tag="U2R41_002527"
     CDS             complement(4476..4826)
                     /locus_tag="U2R41_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889876.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4819..6144)
                     /locus_tag="U2R41_002528"
     CDS             complement(4819..6144)
                     /locus_tag="U2R41_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8889877.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(6313..6897)
                     /locus_tag="U2R41_002529"
     CDS             complement(6313..6897)
                     /locus_tag="U2R41_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8889878.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(7108..7311)
                     /locus_tag="U2R41_002530"
     CDS             complement(7108..7311)
                     /locus_tag="U2R41_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889879.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7321..7743)
                     /locus_tag="U2R41_002531"
     CDS             complement(7321..7743)
                     /locus_tag="U2R41_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889880.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8057..8671
                     /locus_tag="U2R41_002532"
     CDS             8057..8671
                     /locus_tag="U2R41_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8889881.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT         2698 a         1572 c         1318 g         3105 t
ORIGIN      
        1 tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta
       61 tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa
      121 agccccctct attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac
      181 agaacatcgt accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa
      241 gagacgcaat aaattatatc gaagtttacg cactgcctct accacgatta aaggcatgga
      301 agccattcga ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt
      361 ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata ccgtaaggga
      421 ttttattctt tatttaaaac tttgcaacag aaccgtccgt tgcgggtaat accgtaacac
      481 tgaagggcac gcattaaggc gtcatcttaa tgctttcctg taggacactg acggagatta
      541 ccagtctccc gagccttaca ggggcctttc ttttttttgt attttcaatt atcacaattt
      601 atcgtccaaa agtgcagaac gaaaatcaat tcatcacaag catatcagac aattaaattt
      661 aaaacaacta aaaatataca aaaaagtgta attgaatatt agcagcactg cctgaaaaga
      721 agtaatatca ggcttttcag cttggggaaa taagtgaatt acctctcttc atgaattatt
      781 ataactcaaa aaagcacata tgcaaacact agttccctat ttttttgaat gaaaaataac
      841 taaaatatct aacgattatg actttaaaac ctacgtttgt gattgttttc ataaactttt
      901 aatctaaaaa ctccggaata atagctattt atttgtaaca tttgtaataa aattaaaatc
      961 ataaaaaagt tctaatttaa aattgatcat ttttatagtg atcgcttttt atcaactact
     1021 ctagccaatt ttccgtacta actgttggta attcgttaaa tgtttctctt tgttcgtttg
     1081 caagctttgc cgcaatagct ttttcgacaa accaattttt tacataaacg aaaattagat
     1141 tctgcaaatt gtttactttt tcagtttttt gtttttgata agctttcagc aaagtcatat
     1201 ataatccttt gtcagcatca attccataag attccagttc ttcataaaca acaactgttt
     1261 gctctaattc ttcagctttt ttctttgcat tatgaatagt ttggacggtt ttttgagcgt
     1321 cttcgtaagt actagaaaat gtttttatca agtttaatac attttctggt acaaatgtat
     1381 ttatactgtc ctctttcatg atgtcgacga agttatctag taaaacttga ttttgtaact
     1441 gatcagtttc agtgtctatt atgtgtctat tagtgtctat agaattagaa tagaatagat
     1501 tgtctgcgat tttcgcaatt acggttgagt ccttgatatg actggatttt gccgtatttg
     1561 cgattttcgc aattacggtg gtatcattga tttctcgcgg ttttgccgta tttgcgattt
     1621 tcgcaattac ggtggtatca gtattgtctt cctcttccat gattttaaag acgtcttgat
     1681 tcgttacttc tggcttgcct aagtacaata aattaggcat ggattcaatc ttaccgtcac
     1741 gtttatttac tcttcctctc ttctgtttca gaagacctgc tttttctaat tcctttttga
     1801 ttttagatac tttaccttcg cggcattgta acaatttcat taattcttca actgtgaata
     1861 tgaaaaatat attatcatca gaatctaccc agttattttt gactgaataa tcaaatcgat
     1921 ctttaagtag catataagca atttttgcat catttgatag atccatgtat ttttcgttag
     1981 taaaaaatac cttaggcatt tgataatatt tttcacgata aatttctttt gatttgtaat
     2041 aattaaaatc attcatagcc aatcctcttt ctgtaggata gcgttttgtt caaaactatg
     2101 ttacaatgaa ttaaacaaat atatctatcc actatatatt tttctactgc tgataagtcg
     2161 ccaaactttg ttaaagcagt agtctttttt tatctttttt tagtttacct taaaaaataa
     2221 attaaagcaa accgctctat ttcaaggttt aagctcatag taactatatc acgatattac
     2281 aataatatta tatcacgata tcgtaatatt acaatatgaa gataagcgac ttatcagctt
     2341 tgatactact gatttttata aaaaaaatat cacgatatta tgatatcgtg atattgaatg
     2401 taaattaaat tttattttgt ttctttgcta gtttatatcc ttcaatcaag tattcttcag
     2461 gtatggactt taatagcgca tcttctataa cgtctggttt acttttgtct gattcagcaa
     2521 tagtcgtcca atatttgagt gctgacatta gaattggatt tatacgaatg ctatctgctg
     2581 ttcttacact acttccttga tcttttatga ctttacttat atttttcttg gttaattcga
     2641 tttcttctac ttcttttgat ttaaaattct ttttattttt atttcctagt aatctactta
     2701 ctgcagcttg actgcgatta tcttctgcca tcaaaatcac caacttcttt taaatattct
     2761 tctgtaaatg ctttatagtc atcttcagct gttgagggtt tattaaaatt gattagatct
     2821 gatccagcag cttgcgcttc gtcaacggtt attgtcctac gaatataagt tttgaaaatt
     2881 tttgcaccaa tgactctaga aatttcggtt gctgcagacg tcatttcttt agaaatgtta
     2941 taacgcttat tatacttagt aaatagaact cctcgtactt caagcttgtc gttcgtatat
     3001 tcttttaccg tttcaatagt ttcacttaac tttattagtc cttttaatga gaaatacgca
     3061 gcttcaactg gaatgataat ttcatcagaa acagtaaatg cgttgactgt aagcattcct
     3121 aaacttggtg gacaatcaat tataatataa tcatagttgt ccttgattgg ttgaagtact
     3181 cttttaagcc tgtgttcact tcctacttga gtaaattccc tctctgcaga agaaagggct
     3241 atgtcggctg gcaaaacatc aattccttta tagtccagaa tagcatctgt aatgtcagcc
     3301 tcccctttta atacttcata aattgtaggt tggtttgttc catctatatc gaaacctaca
     3361 ttatctgtag catttccctg aggatcaatg tccactaaaa gtacattttt accgtaaaca
     3421 aatttcaaac atgctgctaa attaacgcta gtcgtagttt tagaaactcc acctttttga
     3481 ttggctactg acaatattct tgccattaaa atatccccca ttcatcttat attataatat
     3541 aattatatca taatattgtg atattgcaat atcgtatatg cattatattg taatattgtg
     3601 tgattatttt gttttaaaga tatatatcgg ttcggatata aagattattc atatgttatt
     3661 cgaaattaaa ataatcactt tttattactt taaaattttt tattaatgat aagaaatatg
     3721 ttttgcgagc agcaactaga ataatttaaa cattactaaa ctgtctatat tgttactaat
     3781 aattcccttc ttatagtgca ttttcttaga aaaaaacaat attacgatat catgatatta
     3841 agatatcgta atattataca attgtgattg atattatata tgttttttta tttttttatc
     3901 atgtttaatc ttgaaggaat catattcttt ttctaaagac aggagctttt cataaacatc
     3961 agacatgttt aaactagagg ccaggcttaa atcataaacg aatgaagcag gtaatgattt
     4021 tattctacgt tgtcgggtta tccagcttgc aaccgttcct tgtgggtagg catataactc
     4081 acaaaattgt tctacagtga ttcctaaatt cttgataatg taaacattga tcgggtgcga
     4141 gtaaacaaat gtattgatag acatggtgcg taagcctact ttcttttatt attgcgtttg
     4201 caataatagt ttaacacacg cattattcta gtatctactc ctatttgtaa aaacaaaaaa
     4261 ggattcatag agataactct atagaatccg taaaatacct tatatatata acgggaagat
     4321 ataaaaccta aaattatgat aacctgctgc agcttctaca tagaaagaga agcgctcaac
     4381 accaacctca tttatcaaac gttggccata accccaaatc tcttctgata gctgatcaaa
     4441 ggttatatac tttagctcat attgctggat aatgttcatt ctaatcgact ccatttatta
     4501 tcctggtaaa attcgatatg acgtatttcg tcgtagtgaa ttttgtcttt tccaacgtag
     4561 atccccagct catcactacc taagatcttg ccaatgatat cttctggata gtatccttca
     4621 gcgtttcgtt cttctttttg aatagatact gatctatttt ttatgactgc agcgttcaat
     4681 acttccgcaa tctcttcagt cgtcatttgt tcttttgcta aattttgact ggttcgttgt
     4741 tcattgtcgg catcaatttc ttcagtatga tcagataaat aaaaccctgc ccactttaaa
     4801 cgtccacgat cattgtaatc aatcattttt aattccccca tttccgcccg catgaccacc
     4861 aacaagtgtg cttctagtaa tgcttctagc gccttctaac attgaagaag catgtacaat
     4921 cgacgtaaaa ccatattttt gccgaatctt gtcaataatt ttatctaaat tttcttcgtt
     4981 aatttgcttt tgtggatcac taaataaatc cagctgaagc gaatcggtgt acacaagctt
     5041 gctataggtt attccaacat gccttacttc ttgtccttca taatacttat caaacaagaa
     5101 aagacaataa ttctgcaatt cctttgtatt atctgtgata gggatcttca tctggtgtga
     5161 aaaccctggt ctcgtttcta agatcgacgt gccaatgtgt aaatggacac aacctgtttt
     5221 acagttatgc tgcctaatgc gtatcgctac ttgttccgcc atttctttta ccaccagctc
     5281 gatttcattt cttctagtat aatctcgtgg taacacttgt gaatttccat atgatttttc
     5341 ctttgtcggt tctggtggga ttgctatatc tgttcgatcg atcccattag catggaagta
     5401 cagctgtaat ccaatcacac ctaatctatt cttaatcgta taaggattcc agttagcaag
     5461 gtctctaatg ctcaaaattc ccatttggtt gagccttttt ttcattcgcg aaccaattcc
     5521 ccaaaagtcc gtcatttcgg gaatattcca tactttctca ggaacgtttt cgtacgtcca
     5581 ttccgcaata aatccttcat tatgctttgc ttcattgtct aaggctaatt tagccaaaag
     5641 gggattatcg ccgacgccta ccgtagcaat caaacctaac tcttctttaa tacgttcttg
     5701 gatcatttga gcgagcttct tccttcgctg acttcgtgtt tcttcagtcg taaaaagatt
     5761 cagtgatttt gtcactttta aaatcgattc atcgatcgag taaatcagta gatcttcatc
     5821 agccacatat cttctgaaaa tattatttac ctgcatattt cgcttgatat ataggttcat
     5881 acgtggtgga acaacgtgta gtgttttagg aaatggttgt ggtaagtcac gtggtctact
     5941 cacatttgta ataccatacc gcttttttgc ttcaggagaa gaagctaata tcaatcctga
     6001 accagtatta tcagatcgac tcataacaac aagttctgtt gttaatggat ctaaatttct
     6061 ttctatacat tcgacacttg cgtaaaagct tttcatatcg attaaaaagt aatcatttac
     6121 tggctctttt gaataatcca tcataaataa cactccattc ttccacatta tacaaacata
     6181 tgttcgatgt tgtaaagaag aaatagctaa atatttgaga ataagttatc agtagacact
     6241 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
     6301 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
     6361 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
     6421 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
     6481 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
     6541 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
     6601 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
     6661 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
     6721 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
     6781 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
     6841 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
     6901 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
     6961 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
     7021 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
     7081 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
     7141 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     7201 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     7261 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     7321 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     7381 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     7441 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     7501 tatcgtttta gaagctgctc gagcaagtaa tggagaaact attaaataat tttcagtaac
     7561 agttaagtcg ttagttgggt taccaatggc tattccgttc ctatctaaag gagttaattc
     7621 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     7681 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     7741 cattgtctat cacctcgaaa tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga
     7801 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     7861 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacaca
     7921 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     7981 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     8041 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     8101 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     8161 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     8221 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     8281 cacttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     8341 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     8401 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     8461 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     8521 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     8581 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     8641 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tat
//
LOCUS       DASHWH010000096         8490 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.099, whole genome shotgun sequence.
ACCESSION   DASHWH010000096 DASHWH010000000
VERSION     DASHWH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.099"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..173)
                     /locus_tag="U2R41_002533"
     CDS             complement(<1..173)
                     /locus_tag="U2R41_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8889882.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U2R41_002534"
     CDS             complement(593..1714)
                     /locus_tag="U2R41_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEM8889883.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U2R41_002535"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U2R41_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEM8889884.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U2R41_002536"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U2R41_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEM8889885.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U2R41_002537"
     CDS             complement(4304..5215)
                     /locus_tag="U2R41_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8889886.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U2R41_002538"
     CDS             complement(5232..6239)
                     /locus_tag="U2R41_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8889887.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIVIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U2R41_002539"
     CDS             complement(6236..8365)
                     /locus_tag="U2R41_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8889888.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="U2R41_002540"
     CDS             complement(8370..>8490)
                     /locus_tag="U2R41_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8889889.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2601 a         1726 c         1484 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 acgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DASHWH010000097         8298 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.100, whole genome shotgun sequence.
ACCESSION   DASHWH010000097 DASHWH010000000
VERSION     DASHWH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.100"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            47..679
                     /locus_tag="U2R41_002541"
     CDS             47..679
                     /locus_tag="U2R41_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM8889890.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="U2R41_002542"
     CDS             complement(681..1586)
                     /locus_tag="U2R41_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8889891.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2330)
                     /locus_tag="U2R41_002543"
     CDS             complement(1671..2330)
                     /locus_tag="U2R41_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8889892.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="U2R41_002544"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="U2R41_002544"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEM8889893.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="U2R41_002545"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="U2R41_002545"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEM8889894.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="U2R41_002546"
     CDS             4573..5658
                     /locus_tag="U2R41_002546"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEM8889895.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="U2R41_002547"
     CDS             5655..6719
                     /locus_tag="U2R41_002547"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEM8889896.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="U2R41_002548"
     CDS             complement(6968..7771)
                     /locus_tag="U2R41_002548"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEM8889897.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="U2R41_002549"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="U2R41_002549"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8298)
                     /locus_tag="U2R41_002550"
     CDS             complement(8078..>8298)
                     /locus_tag="U2R41_002550"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM8889898.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2505 a         1740 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgccc
//
LOCUS       DASHWH010000098         8296 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.101, whole genome shotgun sequence.
ACCESSION   DASHWH010000098 DASHWH010000000
VERSION     DASHWH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.101"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(51..1517)
                     /locus_tag="U2R41_002551"
     CDS             complement(51..1517)
                     /locus_tag="U2R41_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEM8889899.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="U2R41_002552"
     CDS             1904..2500
                     /locus_tag="U2R41_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8889900.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="U2R41_002553"
     CDS             complement(2558..3040)
                     /locus_tag="U2R41_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889901.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="U2R41_002554"
     CDS             complement(3146..3343)
                     /locus_tag="U2R41_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889902.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="U2R41_002555"
     CDS             3638..4396
                     /locus_tag="U2R41_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEM8889903.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="U2R41_002556"
     CDS             4456..4884
                     /locus_tag="U2R41_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889904.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="U2R41_002557"
     tRNA            4930..5002
                     /locus_tag="U2R41_002557"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="U2R41_002558"
     CDS             complement(5152..5523)
                     /locus_tag="U2R41_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8889905.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="U2R41_002559"
     CDS             5738..5923
                     /locus_tag="U2R41_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889906.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="U2R41_002560"
     CDS             complement(6006..6194)
                     /locus_tag="U2R41_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889907.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="U2R41_002561"
     CDS             complement(6218..6481)
                     /locus_tag="U2R41_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889908.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="U2R41_002562"
     CDS             6639..6896
                     /locus_tag="U2R41_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEM8889909.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="U2R41_002563"
     CDS             complement(6967..7158)
                     /locus_tag="U2R41_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889910.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="U2R41_002564"
     CDS             complement(7299..7619)
                     /locus_tag="U2R41_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889911.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="U2R41_002565"
     CDS             complement(7642..8076)
                     /locus_tag="U2R41_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8889912.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DASHWH010000099         8065 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.102, whole genome shotgun sequence.
ACCESSION   DASHWH010000099 DASHWH010000000
VERSION     DASHWH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.102"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..882
                     /locus_tag="U2R41_002566"
     CDS             <1..882
                     /locus_tag="U2R41_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM8889913.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="U2R41_002567"
     CDS             930..3059
                     /locus_tag="U2R41_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEM8889914.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="U2R41_002568"
     CDS             3034..3723
                     /locus_tag="U2R41_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889915.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKNVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="U2R41_002569"
     CDS             complement(4131..4817)
                     /locus_tag="U2R41_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8889916.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="U2R41_002570"
     CDS             complement(4953..5147)
                     /locus_tag="U2R41_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889917.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="U2R41_002571"
     CDS             complement(5197..5637)
                     /locus_tag="U2R41_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEM8889918.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="U2R41_002572"
     CDS             complement(5641..6486)
                     /locus_tag="U2R41_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEM8889919.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="U2R41_002573"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="U2R41_002573"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEM8889920.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2356 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaaatgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaa
//
LOCUS       DASHWH010000100         7606 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.103, whole genome shotgun sequence.
ACCESSION   DASHWH010000100 DASHWH010000000
VERSION     DASHWH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.103"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..642)
                     /locus_tag="U2R41_002574"
     CDS             complement(37..642)
                     /locus_tag="U2R41_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEM8889921.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(838..1302)
                     /locus_tag="U2R41_002575"
     CDS             complement(838..1302)
                     /locus_tag="U2R41_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8889922.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1324..1644)
                     /locus_tag="U2R41_002576"
     CDS             complement(1324..1644)
                     /locus_tag="U2R41_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM8889923.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="U2R41_002577"
     CDS             complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="U2R41_002577"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEM8889924.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3066..3416
                     /locus_tag="U2R41_002578"
     CDS             3066..3416
                     /locus_tag="U2R41_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM8889925.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            3601..4554
                     /locus_tag="U2R41_002579"
     CDS             3601..4554
                     /locus_tag="U2R41_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8889926.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            4567..6354
                     /locus_tag="U2R41_002580"
     CDS             4567..6354
                     /locus_tag="U2R41_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889927.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            6341..7543
                     /locus_tag="U2R41_002581"
     CDS             6341..7543
                     /locus_tag="U2R41_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889928.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2168 a         1446 c         1366 g         2626 t
ORIGIN      
        1 aaaaagaggt tgtgacaaag acttgtcaca acctctttat tttggaaata cttctccgac
       61 tacggcatcc tcaggcaata ctaagattcc ttttttttct ggagcatttg gcaaatgcaa
      121 ttcacgagca gaacaaatca ttccatagct ggctacccct cgcaaagttc ctggccagat
      181 catcattcca tctggcatca tggctcctgg ttttgcaacg acaacttttt gtccagcttc
      241 gatatttggt gcaccgcaaa caatttgcaa tactgtccca ttgtccactt ctgtctcagt
      301 aatcgataaa tggtcagagt caggatgcgg gacgcatgtt tttacatacc cgacaacgat
      361 cttcggttca tgatcagctt caagctgttc ttcgaatcct gcttctttaa tcaatcgatt
      421 caatttcgct aattcttcat ctgttaagct tacttggccg ttcccttcaa ttgttagatg
      481 atcggagata tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc
      541 aacattccat ttacgttcga cagtgttttc tgttccttta tcgtcggcta tgataaccat
      601 caaagtgtct ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc
      661 ctttgctgag atttatctta acaaaaaatt gcattcactg catgagattt ttttcatgct
      721 ttatttccga agaagcttct tctggaacac catttattcg taacaaggga ctgtgacaag
      781 acttttgtca cagtcccttt actgtttgat taaaaaggtt atacaagttc cacttgctta
      841 attactcggc tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat
      901 gacgtagctg gctacacttc ctgtcataaa gcgttcgact gcatttagtc cagactgccc
      961 aaccatgatt agatcgattg catatttttt tggcaatttg actgccatgg attcacgaac
     1021 agagccgtat gcaataattc cttctatatt tgtgtaatca acagattttc catattcttt
     1081 acattcttcg atcatttgct tggctgtttc agtttgctga tcgactagat tttgatcaat
     1141 aggagcatag cccataaaat tataaaattg ctgatcgatt acgtttgcta catatacttt
     1201 cccattatta cggcgagcca cttctaacgc ttttttgaaa gcagcaagtg cttgttcact
     1261 accgtcaata cctactaaaa tgttcttata ttcttgattc atcttgaccc ctcctaacag
     1321 atgtcagcct tttattacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc
     1381 tttattcact agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc
     1441 aaaaatgttc cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg
     1501 ccaatcagaa aagtcatttt cgatttcagg catttgcggt ttgataaaac ggcagtctcc
     1561 gcaccagccg gcagtaaaga aaaatacact ttttccagtt tctacgtagc ctgctaattc
     1621 ttctaaacta tttgggataa tcattctttt tcctctactt tctgtttatg tatttttcaa
     1681 ttaaattata cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat
     1741 ctgacaaaag gttgcttttg ccagatttta tagtttcact atcgcgttat ttcccgtata
     1801 cgatcgtgtt cactgttgat ttatctaaac ctttcaatgt ttgaatcaac atttctcgtg
     1861 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     1921 cgccaatcac tgtacttggg attccttcat tttgtgtatg agcagcacct gcatctgtac
     1981 cgccttttga aacaaagtat tgataaggga tgttgtttgt ttcagcgata tctagcaagt
     2041 actcacgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     2101 ctcctaaatg tccgaatgtg ccatttttcg tcactgtgtc atctgctgca gagcagtcta
     2161 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     2221 cttcttcttg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgtaacg
     2281 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccacgat ttgctgatga
     2341 tattcttccc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     2401 gaacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatctg
     2461 ttacttctac tgatttttgt ccgcttgttc ctcgtaataa atgtggggga acggaagaag
     2521 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgttga gcagaaacaa
     2581 cgtaagggtt ccatccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     2641 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacgaggt gcatccgctt
     2701 cttcatggtg gcgcagccca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     2761 gcggttccat atgactttcc atataggcgc gaacgtcttg ttcaaaaccg cttgttcctt
     2821 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     2881 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     2941 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     3001 aaaatatgag agtgtggtaa aataaacaca aagagtagca agtgagaaga aggaggtata
     3061 tccacatgta tgaagaaagc gaattaagct attttaaagg cgggttagcc gttgggatcg
     3121 gtctaggatt attgagtggg gtagcttcaa cgatttggta tcatcgcaaa aaagtgttat
     3181 cagcagacca agtgcttgaa aatatcaaaa catctttttt aaaagaaggc gcaatcgaag
     3241 gtgcttggat ctcttttgaa aaagaaccta ttcggaaatt cgccgttcgg tcgagtgctt
     3301 atcggggtgg gctttcccgg atagaagatg gcgaaactgt ttattatgaa tttttagcag
     3361 atgctcatac aggaaccgta ttagacattt caagaaaaaa gggcgcaata gattaatacg
     3421 tcaaaaaaat gaaataacga atacgcaccc actatctaaa agagaggtat cctatccttc
     3481 ttttatagtg ggtgattttt ataaaaatag ggctatttcc tcttggaata gctagtattt
     3541 gtagttatta tacggtattc ttgaaataag attagtttta ttatataagg agagtgacaa
     3601 atggaatcac gtgaaaaaat tgccgtgctc attccttgtt ataacgaaga ggcgacaatt
     3661 tcaactgtta ttgaagattt taaaagggaa ttaccagaag cagatattta tgtctatgat
     3721 aataattcga ccgatcgaac atacgaattg gccgttcaag gcggagcaat cgtaaagaaa
     3781 gaaccacgtc aaggaaaagg gaatgtaatc cgtcaaatgt tttttgatat tgatgccgat
     3841 tattatttga tggtagatgg tgatgatact tatcctgcag aagctgcaca tggtttgctt
     3901 gagaaattac gttctggcga agcagatatg gtcattggcg atcgtctatc aaatggaacc
     3961 tattttgatg aaaataagcg tcctttccat gattttggga ataatctggt taaaaataca
     4021 attacttact tatataaaac aaaaattcgc gatgtcatga ccggctatcg aggattcaat
     4081 cgaatttttg tcaaaagctt ccctattatg agttctggtt ttcaaatcga aacagaattg
     4141 acgattcatg ctttagataa aaaattcaag ttcgttgaga ttccaatcga ttatcgcgat
     4201 cgtcctgaag gtagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     4261 atgatcatca aaatgtgcaa agattataaa cctttgcttt tctttgggat ctggaccatc
     4321 ttcttctttt tatttggatt ggtcatgggg attcctgtga ttcgtgaata tatgctgaca
     4381 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagccctt
     4441 ctttcattag taacagggtt gattttggat acggtcgtca caaatgccaa gaaagaatat
     4501 gaattgaatt tataccatgt ctaccacgaa tatcaaaata aagaaatgaa gtaggaggcc
     4561 ttttttatgc ataagaaaaa ggagaatcta tttcctactt tttttgcaac tttatttgcg
     4621 tcaggttttg ctttttttac ttcttatggt ctaggaactt tgaatccaac agtaatcatt
     4681 ggtttagcca tggttttgct acttgctttt aagcaatatg gaatagagtt gttttcaata
     4741 aaaaacaaag ttgcagctat tgcccgattg tttttttctg cagcttttgc tcttacactt
     4801 tctattggca gacagataca atttcagtat gtctcagcaa actatacaaa gacaacgatt
     4861 gctgatggaa gtatttttta ctttcttgtt tatttctcca tttttgtgta ttctttttta
     4921 tttttacaat cgatttattt atcaatgcca aaactggaaa cattttttac tgctcaaaac
     4981 acacaggctt tctcttcaag aaaatatttt ttattcttta ctgtcttttt agttctgtgt
     5041 tggcttcctt atctgttggc attgtatcct ggagtggttt tgcctgattc gttaagttcg
     5101 gtcgcccaat ctatgggtga tgttcctata agcaatcatc atccagtctt attcacctta
     5161 ctggtcaagc tatttgttca tgatttacca actgatacga tcaatcacgg tgtattttta
     5221 ttttcgattt ttcaaacatt gataacagct tcagcgataa gttattctct ttgctggctg
     5281 ttaaaaagaa acatccattt ttttccggta ctcattagtt tatgctattt tgcatttgca
     5341 ccggtttttc caatctacgc cttgaacatg caaaaagata ctttgttctc tatcgcttgc
     5401 tttttatttt ccttagcggt ttttgacttt atttttcaac gagaaaagcc aacgattctc
     5461 aatcagattt acgtaatcat ccttgcttta ctgaccacct atctaagaaa taatgggatt
     5521 tatgtagtaa ccggcacagg tttgatttgc ggcttgatta ttttgaaaca aaaaaggtat
     5581 caagcattct ttcaagtggt gctgggttat acagtgattg ctggactact catccacccg
     5641 ttgatggctc gttatgctat tcctacagaa actgctgaac aattaggtgt tcctttgcag
     5701 caaatttcta gaactattgt tatggatggt gacttgtcta agaaagataa agcattcatg
     5761 aatcagctac tcccgataaa agattatgaa gcgtattctc ctagtcttgc agactcaatc
     5821 aagtggcata aaaattttaa caatgagttt ttgaatacac ataaaaaaga atttctatca
     5881 ctatgggcaa gaaatttacc aagtaatata aaaatctata cagatgctta tattttagaa
     5941 acgttcgggt tctgggtacc tggtacaaaa aatagttacg gctttttaga tactcgtgta
     6001 aacacgaata attatggtat gaagcaagta gatttgattg aacatcttac tggattgtca
     6061 ggaatgaaaa aaatcatcga tgctcgtgat ttctttggtt ccggcacatt actgtggtta
     6121 gctatcggca gtgcactttt agcaatcttt aagaaaaaat atcggtattt actccttttt
     6181 cttcccggta tgcttgtttt tgctactatt atgattgcta ctcctgtggc ttttagttta
     6241 cggtatgtgt ttgttttagc attaggtcta cctgtttata tcctctctcc ttttttacta
     6301 gataaaagag aaaaaatgaa aacagatata ggagatactc atgaaactat ttaaaaagat
     6361 attttttgct tttgttctct tgatcgctag ctattgcgta ctactgacaa ttactttttt
     6421 cattccgcag cactctattg aatcgaatgc agaaaaatct ctacagatgg ttgaaaaaga
     6481 aggaatgtat ccttctatca atcaaggaaa tatgctaggc actcggttag ataattttac
     6541 tgatcatttg atgatacgaa aaacaaaagc agatcctgaa ttgaactcct tggagaacgc
     6601 gatgtctatg gcggattatc cccgttattg gcatggttat cagctttttt tacgaccttt
     6661 actgctaggg atgagtttgg ggagtattcg aatgatttac gcagccattc tttttctgct
     6721 gattggattg acgagctact ttttaaccaa gtgtagcgac atctatttaa cgacagcctt
     6781 gttgatttcg cttgctatag gaaatgctgc aacctttttc ttcagtatgc aattttcaaa
     6841 tatttggatt ttgactttat tgtctgtatt actattttta acaaaaccac aactctccaa
     6901 caccaaccaa caattattct tgtatttctt tgtcattggt tctttagcta acttttttga
     6961 cttgttgaca acaccggtca tctcttgggg gatacctgtg atcattattt attatgtaac
     7021 aaataagtat ttaatggata aacgcagttc attgagcaaa caagtaggca gttttgtttt
     7081 taccggtatc ttttggggat taggctatgg tttgacatgg gtggctaaat ggatcctatc
     7141 atcgatcata ttgaacaaaa acattgtaaa agatgcaatc aaccaaatcc tctttcgaac
     7201 ggaaggaaac gacaaatacc cgttgcatcg attagagatg ctgaaaagca atattagatt
     7261 gatgtatcca aaagtagcaa tattattgct attgattact tgtcttgttt ttctctattt
     7321 tgcttatcgt aaacgaaata gctggcgtcc gttccaactt ctagttttgt ttgttttagc
     7381 tgctgtaacg ccgtatattt ggtacaatat tttagctaat cactcacaga ttcattattg
     7441 gtttacgtat cgtacgcaaa tcatcacaag ttttgctata cttagtgctt tcgccttttt
     7501 gatccctcct gagaaatcaa aagacgaact aaattacttt taagaatacc gatgtactaa
     7561 ttcattttta aacataaaaa ctccagctgt cagagatagc cggagt
//
LOCUS       DASHWH010000101         7598 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.104, whole genome shotgun sequence.
ACCESSION   DASHWH010000101 DASHWH010000000
VERSION     DASHWH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.104"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(941..1354)
                     /locus_tag="U2R41_002582"
     CDS             complement(941..1354)
                     /locus_tag="U2R41_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEM8889929.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="U2R41_002583"
     CDS             complement(1810..2295)
                     /locus_tag="U2R41_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889930.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="U2R41_002584"
     CDS             complement(2434..2649)
                     /locus_tag="U2R41_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEM8889931.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="U2R41_002585"
     CDS             2873..3673
                     /locus_tag="U2R41_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8889932.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="U2R41_002586"
     CDS             3692..5560
                     /locus_tag="U2R41_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8889933.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="U2R41_002587"
     CDS             5553..6044
                     /locus_tag="U2R41_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEM8889934.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="U2R41_002588"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="U2R41_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEM8889935.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="U2R41_002589"
     CDS             6604..>7598
                     /locus_tag="U2R41_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEM8889936.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DASHWH010000102         7402 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.106, whole genome shotgun sequence.
ACCESSION   DASHWH010000102 DASHWH010000000
VERSION     DASHWH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.106"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            69..152
                     /locus_tag="U2R41_002590"
     CDS             69..152
                     /locus_tag="U2R41_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM8889937.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="U2R41_002591"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="U2R41_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM8889938.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="U2R41_002592"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="U2R41_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1607..2272
                     /locus_tag="U2R41_002593"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="U2R41_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="U2R41_002594"
     CDS             2308..2553
                     /locus_tag="U2R41_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8889939.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="U2R41_002595"
     CDS             2656..3525
                     /locus_tag="U2R41_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM8889940.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="U2R41_002596"
     CDS             3506..4240
                     /locus_tag="U2R41_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8889941.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5185
                     /gene="ant(6)"
                     /locus_tag="U2R41_002597"
                     /pseudo
     CDS             4273..5185
                     /gene="ant(6)"
                     /locus_tag="U2R41_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5182..5724
                     /gene="sat4"
                     /locus_tag="U2R41_002598"
     CDS             5182..5724
                     /gene="sat4"
                     /locus_tag="U2R41_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM8889942.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5817..6611
                     /locus_tag="U2R41_002599"
                     /note="aph(3')-IIIa"
     CDS             5817..6611
                     /locus_tag="U2R41_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM8889943.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6887..>7402
                     /locus_tag="U2R41_002600"
     CDS             6887..>7402
                     /locus_tag="U2R41_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8889944.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2593 a         1119 c         1556 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat
     4381 acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt
     4441 tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga
     4501 ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga
     4561 ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg
     4621 cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc
     4681 gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa
     4741 tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt
     4801 tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa
     4861 ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag
     4921 gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga
     4981 aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt
     5041 ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag
     5101 ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga
     5161 tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc
     5221 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     5281 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
     5341 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     5401 atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg
     5461 aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata
     5521 ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta
     5581 atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc
     5641 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     5701 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     5761 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     5821 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     5881 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     5941 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     6001 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     6061 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     6121 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     6181 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     6241 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     6301 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     6361 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     6421 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     6481 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     6541 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     6601 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     6661 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     6721 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     6781 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     6841 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     6901 cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat
     6961 tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga
     7021 agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct
     7081 acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt
     7141 tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat
     7201 caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa
     7261 cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg cagcaattca
     7321 gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga
     7381 accggaaagc agaatgatag ag
//
LOCUS       DASHWH010000103         6971 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.107, whole genome shotgun sequence.
ACCESSION   DASHWH010000103 DASHWH010000000
VERSION     DASHWH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.107"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..415
                     /locus_tag="U2R41_002601"
                     /pseudo
     CDS             1..415
                     /locus_tag="U2R41_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            431..580
                     /locus_tag="U2R41_002602"
     CDS             431..580
                     /locus_tag="U2R41_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889945.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            661..1257
                     /locus_tag="U2R41_002603"
     CDS             661..1257
                     /locus_tag="U2R41_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM8889946.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1270..2169
                     /locus_tag="U2R41_002604"
     CDS             1270..2169
                     /locus_tag="U2R41_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM8889947.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2172..2303
                     /gene="ssb"
                     /locus_tag="U2R41_002605"
     CDS             2172..2303
                     /gene="ssb"
                     /locus_tag="U2R41_002605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8889948.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2325..2657
                     /gene="ssb"
                     /locus_tag="U2R41_002606"
     CDS             2325..2657
                     /gene="ssb"
                     /locus_tag="U2R41_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8889949.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2702..2935
                     /locus_tag="U2R41_002607"
     CDS             2702..2935
                     /locus_tag="U2R41_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889950.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3094..3216)
                     /locus_tag="U2R41_002608"
     CDS             complement(3094..3216)
                     /locus_tag="U2R41_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEM8889951.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3406..3630
                     /locus_tag="U2R41_002609"
     CDS             3406..3630
                     /locus_tag="U2R41_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889952.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4073..4279
                     /locus_tag="U2R41_002610"
     CDS             4073..4279
                     /locus_tag="U2R41_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEM8889953.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4279..4530
                     /locus_tag="U2R41_002611"
     CDS             4279..4530
                     /locus_tag="U2R41_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889954.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4546..4701
                     /locus_tag="U2R41_002612"
     CDS             4546..4701
                     /locus_tag="U2R41_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889955.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            4711..4983
                     /locus_tag="U2R41_002613"
     CDS             4711..4983
                     /locus_tag="U2R41_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889956.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5428..5607
                     /locus_tag="U2R41_002614"
     CDS             5428..5607
                     /locus_tag="U2R41_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889957.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5666..6022
                     /locus_tag="U2R41_002615"
     CDS             5666..6022
                     /locus_tag="U2R41_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889958.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2497 a         1129 c         1387 g         1958 t
ORIGIN      
        1 acgagcgccg aaatgaccgc caaatgggaa agctacttgt taaagatcgg tgaacgaaaa
       61 ggaacacaaa ccacttttct agccaatatc caaaaatttg tctctcactt attggaagtc
      121 gtaccagggc aaatccaatc cactgatttt ggatcgacgt tacaagaagt gaaagcagcc
      181 agtgaaaagc aagaagccgc gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa
      241 gtcttactct atcaaaaggt ggcagcttgt acgtctgaag cctgtgattt taaaaggtca
      301 gaagggttca tttgaagcaa cgattgtttt aaaagaagac ttcactacag gctttgaatt
      361 ttctgaaaag aaaaaaacga acttcaagaa aagaacacgc cgcactgcaa aataatggag
      421 ggttaatctt atgcgtaata ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa
      481 aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc aattgaagaa
      541 ccagcagagg attcaaaaag aaatcgaatt agatcgataa aatcttccta actattgtat
      601 acgtataaac ttatgttata ctgtttttat aagtttatac gtatacaaag gagggagaaa
      661 atgacaaaag caatggtata caagctgcta gaagaattta atggaacttt ggctttgaag
      721 gatgccaaaa aagcgggcat ttctcctgta acgatcaaac gtatggtaga tcgaggggag
      781 ctggatcgag agtatccagg ttttttcacc ttacctggtc aatttcctga tgaattgttt
      841 atggcgcaaa aaaaatatga acgcggcatt atttcacata ttaccgcgtt agatttgtat
      901 gatttaacag atatgatccc tagacagatc gatctgactg taccttacgg ttatcacgtt
      961 tctgaaaaag gattgaagga attcgcagtt gagctacact atacaaaagc ggaatggtat
     1021 gaattaggta agattgaaat aaaaagtcgc tatgggaatc ctattatagc ttatgatcca
     1081 gaaaaaacct tgtgtgacat ttggaatccc tggtataacg ttgaggatga aatcaaagta
     1141 aaagcaatca aaaactacat ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat
     1201 cgacgaattt tgcctacaga taaaacgatg cgctcttata ttatggcttt aaattaaagg
     1261 agaatacaca tgacacctac gcaattaaaa gcaaaagtga agaatatttc tagagaaaaa
     1321 gaagttgatc cgcagcttgt tttaagacat tttatgatgg aaaaattctt ggaaaaaatc
     1381 tcagagtctc cttatcgtga aaattttgtg ttaaaaggcg gcttcttaat tggctcaaag
     1441 tatggcattg aaaatcgaac aactaaagac attgatacga ctttacggga aatgaaagta
     1501 accaaagaaa cattgacaac ggttctaaac gatatttttt caacgccgac gaaagaaggc
     1561 attcaatttg agattcaagg aatgaaagaa acgagggaag ctgattatta tccagggttt
     1621 agcttacgtg tactggcaca tttggaaaac atgcggccag attttaaggt agatgttact
     1681 acaggtgact cgatctatcc tgcgacgatc actcacagtc ataaattgat gtttgaggat
     1741 cgaacgattc ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa attaagtgca
     1801 acctttgatt ttttaacaga taattcgaga ggaaaagatt tttatgatct ctatactatt
     1861 cctaaaatgg agaagatcga tgagaagacc ttgaaagata gtgtgagaaa tacatttaca
     1921 cgtcgaggaa aaactgatcc gttaaagata tactatgaaa aggatatgga tgtactgaaa
     1981 aatagtcacg agctaaaaga tacctggaag aaatatcaaa aaagcaatcc ttatgctgcg
     2041 tcgatcacgt acgatgagac aatggattcg attagtaatt tgatggaaaa agtgattcac
     2101 gtcgaagaaa aagagcgaaa actagcttat caaagacacc aacagatgaa tagagaaatg
     2161 gagagataaa gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcctgatt
     2221 tacgttatac cgcaagtggt actgcagtag cgacttttac cctagccgtc aaccgaaatt
     2281 tcacgaatca aaacggcaat tgagaagccg attttattaa ctgcgtgatt tggcgcaaac
     2341 cagcagaaac aatggcgaca ttagctaaaa aaggtagctt gattggtgta gttgggcgga
     2401 ttcaaacgcg gacttacgac aaccagcaag gacaacgtgt atatgtgact gaagttgtag
     2461 cagataactt tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc
     2521 aatcgtccac cagtccaagc accgccacct ttgaacaacg gaatacagcg acgcctaaca
     2581 acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat attagcgatg
     2641 atgatctacc gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa ttagtctgac
     2701 gatggatcta ttgatacccg taattgcatt tatagacggg atttctttgg gcgtcactga
     2761 aaatcctttg gtaaatagaa ttgcgaaagg atcactattt cttgtattcg tgtatcttgt
     2821 ctgttcttat cttttaaaaa gagcaggaaa ggataattcc ttttggcgat tgactaaagt
     2881 cacagtagca gcgacagctg ttatacttac ggctattcta ccgctttata tgtagagtta
     2941 acgggaaatc gttgaacttc ttgcgaaata ttgctataat gaacatgaaa aagaggagtt
     3001 gcgctaacaa cccctcatta acagcaccga ttaaagaacg gtggcagaat attagttatt
     3061 tggaaataac cgctgacctg ctaaagtaga cggttatttc tttttgtcat ttttaagcat
     3121 atttacaatc aaactaatca aagcaatggt aaacatacca aaaccaagaa tggtttgaat
     3181 tgtctcatat gcggacaaaa ggcttactcc tttcatagat tttagcactt acattcataa
     3241 gcaccaccac ctttccagaa gtcgccaccg tcttcaaact ttactgtctt gactattata
     3301 gcatatctaa aatcgaataa aaattctcaa ttttagctgt ttttgctata attttagcga
     3361 ggtgatcgtg atggaacaat atattggttt actcgataaa ttagggtgat caagacacag
     3421 ccattgcttg tgcgatttac attacgcacg agtcatgaac caatcaattg tgtagtggcc
     3481 aatatagaaa tagcggataa attgctgatt ttggaagaag gcaagtatac tatttcagtg
     3541 acaggtcact tcaataaacg aaagcaacta gtcattgaat caatgcaaat tagaaaccct
     3601 gatcatttta cgcgatcgat gagcatataa aagaagagct gcagtcctaa atgaggattg
     3661 tagctctttt tgttttacat agaaacagta aaaatgaaaa ggcctgttta ggaaggcaca
     3721 agcgccttct caattgaaag acctttctct cactccaacc gccaccgccg ttcgagaaga
     3781 aaggaagtga acgctacgcg ttcactaaga taaagggtct tgcatagcaa gacaaaatag
     3841 aaaaaatttc ctctcaccaa aaggttcgct cctgttcgtt atctctttat tgatttgtca
     3901 gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg
     3961 ctgcgcgccc ttgtcttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga
     4021 aagaactaac gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa
     4081 gaaatttgat tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca
     4141 tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc aaaagctatt gtttgtatca
     4201 atgtccaaca tgcgggaaaa aatatacctt gtcttatggc gattatattt taattgatga
     4261 gaaaggaaag aaacgctgat gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt
     4321 caaattttgg tggacaaaga agtcatttct gaaaaagtag cagaagtttt acttattccg
     4381 cggtactgga cgatagaaac ccctttagta gaggatgcac cgactatgct atttgaagac
     4441 ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa acttggaaac ttatgaaaac
     4501 gaatattggt taaccaaatt agagaactaa aaaggagaaa aaaatatggc tcaaaattac
     4561 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
     4621 ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct
     4681 gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca
     4741 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
     4801 gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat
     4861 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
     4921 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
     4981 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
     5041 aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa cgcctagtaa
     5101 ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
     5161 gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag
     5221 attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct
     5281 gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg
     5341 tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg
     5401 ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc
     5461 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa
     5521 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
     5581 tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata
     5641 tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa
     5701 gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag
     5761 agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg
     5821 atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat
     5881 tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct
     5941 aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga
     6001 cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta
     6061 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
     6121 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
     6181 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
     6241 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
     6301 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
     6361 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
     6421 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
     6481 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
     6541 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
     6601 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
     6661 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
     6721 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
     6781 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
     6841 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
     6901 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
     6961 aaaggttttt a
//
LOCUS       DASHWH010000104         6643 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.108, whole genome shotgun sequence.
ACCESSION   DASHWH010000104 DASHWH010000000
VERSION     DASHWH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.108"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1..859)
                     /locus_tag="U2R41_002616"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="U2R41_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="U2R41_002617"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="U2R41_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEM8889959.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="U2R41_002618"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="U2R41_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="U2R41_002619"
     CDS             complement(2143..2373)
                     /locus_tag="U2R41_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEM8889960.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="U2R41_002620"
     CDS             complement(2603..3421)
                     /locus_tag="U2R41_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8889961.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="U2R41_002621"
     CDS             3582..4271
                     /locus_tag="U2R41_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM8889962.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="U2R41_002622"
     CDS             4285..5787
                     /locus_tag="U2R41_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8889963.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="U2R41_002623"
     CDS             5800..6276
                     /locus_tag="U2R41_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8889964.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="U2R41_002624"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="U2R41_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DASHWH010000105         6462 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.109, whole genome shotgun sequence.
ACCESSION   DASHWH010000105 DASHWH010000000
VERSION     DASHWH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.109"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(109..564)
                     /locus_tag="U2R41_002625"
     CDS             complement(109..564)
                     /locus_tag="U2R41_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889965.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="U2R41_002626"
     CDS             complement(718..1926)
                     /locus_tag="U2R41_002626"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEM8889966.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="U2R41_002627"
     CDS             complement(2038..2370)
                     /locus_tag="U2R41_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM8889967.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKHE"
     gene            2518..3393
                     /locus_tag="U2R41_002628"
     CDS             2518..3393
                     /locus_tag="U2R41_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8889968.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="U2R41_002629"
     CDS             complement(3475..4056)
                     /locus_tag="U2R41_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEM8889969.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="U2R41_002630"
     CDS             complement(4062..5321)
                     /locus_tag="U2R41_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM8889970.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="U2R41_002631"
     CDS             complement(5323..6369)
                     /locus_tag="U2R41_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889971.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1800 a         1336 c         1103 g         2223 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtgctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aa
//
LOCUS       DASHWH010000106         6410 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.110, whole genome shotgun sequence.
ACCESSION   DASHWH010000106 DASHWH010000000
VERSION     DASHWH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.110"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            75..1379
                     /locus_tag="U2R41_002632"
     CDS             75..1379
                     /locus_tag="U2R41_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM8889972.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="U2R41_002633"
     CDS             complement(1471..1836)
                     /locus_tag="U2R41_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM8889973.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="U2R41_002634"
     CDS             complement(1833..2198)
                     /locus_tag="U2R41_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM8889974.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="U2R41_002635"
     CDS             complement(2390..2638)
                     /locus_tag="U2R41_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889975.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="U2R41_002636"
     CDS             2637..2765
                     /locus_tag="U2R41_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEM8889976.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="U2R41_002637"
     CDS             2822..4516
                     /locus_tag="U2R41_002637"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEM8889977.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4520..4639
                     /locus_tag="U2R41_002638"
                     /pseudo
     CDS             4520..4639
                     /locus_tag="U2R41_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4616..4789
                     /locus_tag="U2R41_002639"
     CDS             4616..4789
                     /locus_tag="U2R41_002639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889978.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5167..6300
                     /locus_tag="U2R41_002640"
     CDS             5167..6300
                     /locus_tag="U2R41_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEM8889979.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2121 a         1222 c         1169 g         1898 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc
     4441 acgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 cattttttca acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
//
LOCUS       DASHWH010000107         6343 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.113, whole genome shotgun sequence.
ACCESSION   DASHWH010000107 DASHWH010000000
VERSION     DASHWH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.113"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            330..1175
                     /locus_tag="U2R41_002641"
     CDS             330..1175
                     /locus_tag="U2R41_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8889980.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="U2R41_002642"
     CDS             1456..2676
                     /locus_tag="U2R41_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEM8889981.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSIKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="U2R41_002643"
     CDS             2673..5393
                     /locus_tag="U2R41_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8889982.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="U2R41_002644"
     CDS             5377..6321
                     /locus_tag="U2R41_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8889983.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2364 a          980 c         1225 g         1774 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcaataaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttc
//
LOCUS       DASHWH010000108         6083 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.114, whole genome shotgun sequence.
ACCESSION   DASHWH010000108 DASHWH010000000
VERSION     DASHWH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.114"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            292..1479
                     /locus_tag="U2R41_002645"
     CDS             292..1479
                     /locus_tag="U2R41_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM8889984.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            complement(2438..2731)
                     /locus_tag="U2R41_002646"
     CDS             complement(2438..2731)
                     /locus_tag="U2R41_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEM8889985.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3134..4312)
                     /locus_tag="U2R41_002647"
     CDS             complement(3134..4312)
                     /locus_tag="U2R41_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8889986.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(4416..4730)
                     /locus_tag="U2R41_002648"
     CDS             complement(4416..4730)
                     /locus_tag="U2R41_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889987.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            4837..5052
                     /locus_tag="U2R41_002649"
     CDS             4837..5052
                     /locus_tag="U2R41_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8889988.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(5352..5594)
                     /locus_tag="U2R41_002650"
     CDS             complement(5352..5594)
                     /locus_tag="U2R41_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8889989.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(5581..6009)
                     /locus_tag="U2R41_002651"
     CDS             complement(5581..6009)
                     /locus_tag="U2R41_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8889990.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1891 a         1143 c          981 g         2068 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta cttgactcta tatagttagt ctctcaataa aaaatcatca
     1621 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
     1681 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
     1741 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
     1801 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
     1861 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
     1921 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
     1981 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
     2041 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
     2101 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
     2161 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
     2221 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
     2281 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
     2341 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
     2401 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
     2461 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
     2521 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
     2581 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
     2641 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
     2701 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
     2761 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
     2821 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
     2881 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
     2941 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
     3001 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
     3061 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
     3121 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
     3181 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
     3241 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
     3301 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
     3361 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
     3421 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
     3481 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
     3541 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
     3601 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
     3661 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
     3721 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
     3781 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
     3841 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
     3901 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
     3961 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
     4021 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
     4081 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
     4141 tgcttgcaac aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt
     4201 gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg
     4261 tgatcctacc agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat
     4321 tcctcctatt ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt
     4381 catcattgta ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat
     4441 ttttctcgat agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa
     4501 tagtgtcctc gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc
     4561 agtgtactat cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt
     4621 aaatgactga ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa
     4681 tagaactcta tttccagaga attaacattt gttttaactt ccaactccat cacccttctc
     4741 tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac
     4801 tccatcaaaa cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata
     4861 aaaataatta aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga
     4921 atcaacttga gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat
     4981 cttgccgatg ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa
     5041 atatggaaat agtagttttt ctgatctatc agctgttttg ccaaagcaca taggaatctc
     5101 acctcccccc agtaatggcg ggccgatttg gttacggaag tatcattatc gtagtcgcga
     5161 ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc atggcgtaac tagagaatca
     5221 aaatctacat gtactgatag atttgctatt catttgtcaa agagcgctag gctattttca
     5281 gcctatggaa atcctttatt ttgacccgat aaaaaacttc catagaatga aaaggaaagg
     5341 gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct tctcaatcaa ttttgtttcc
     5401 agttgtctac gcatatattc atctacatac ataaccattc ctttttcttc ttcataaact
     5461 ggtttcatgg ctaaccgtga aatataattt tgatattttg acagaacgat ggacatggca
     5521 attgaatcac cagaagctgc ggctttgatt gtcgttagtg gtatgaatgg tatatatcgt
     5581 ttattttgct tcatatttga cacccaaata ctttcctagc tcttccaaag ccatttgtcg
     5641 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
     5701 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
     5761 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
     5821 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
     5881 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
     5941 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
     6001 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
     6061 tccattatta ctacaatcta gga
//
LOCUS       DASHWH010000109         6044 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.115, whole genome shotgun sequence.
ACCESSION   DASHWH010000109 DASHWH010000000
VERSION     DASHWH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.115"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..746
                     /locus_tag="U2R41_002652"
     CDS             99..746
                     /locus_tag="U2R41_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM8889991.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U2R41_002653"
     CDS             1139..3784
                     /locus_tag="U2R41_002653"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEM8889992.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U2R41_002654"
     CDS             4094..5407
                     /locus_tag="U2R41_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEM8889993.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..>6044
                     /locus_tag="U2R41_002655"
     CDS             5397..>6044
                     /locus_tag="U2R41_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8889994.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALL"
BASE COUNT         1999 a         1027 c         1320 g         1698 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gtta
//
LOCUS       DASHWH010000110         5905 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.116, whole genome shotgun sequence.
ACCESSION   DASHWH010000110 DASHWH010000000
VERSION     DASHWH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.116"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(93..665)
                     /locus_tag="U2R41_002656"
     CDS             complement(93..665)
                     /locus_tag="U2R41_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8889995.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(672..1334)
                     /gene="rpe"
                     /locus_tag="U2R41_002657"
     CDS             complement(672..1334)
                     /gene="rpe"
                     /locus_tag="U2R41_002657"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM8889996.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1350..1865)
                     /locus_tag="U2R41_002658"
     CDS             complement(1350..1865)
                     /locus_tag="U2R41_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEM8889997.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1867..2151)
                     /locus_tag="U2R41_002659"
     CDS             complement(1867..2151)
                     /locus_tag="U2R41_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8889998.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2185..3513)
                     /locus_tag="U2R41_002660"
     CDS             complement(2185..3513)
                     /locus_tag="U2R41_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEM8889999.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3527..3982)
                     /locus_tag="U2R41_002661"
     CDS             complement(3527..3982)
                     /locus_tag="U2R41_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8890000.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3985..>5905)
                     /locus_tag="U2R41_002662"
     CDS             complement(3985..>5905)
                     /locus_tag="U2R41_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8890001.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1917 a         1147 c          836 g         2005 t
ORIGIN      
        1 ttattaagtt atgtctaatc aaataaaaaa accattctca tatttgctga ataattagca
       61 tttagagaat ggttttttat ctttaaaatc tactactcaa aatctgcatg tcgagcaaac
      121 atcgtttctg agctttcacc tgttttatcc attaaagata acactaaact atcaaaggtg
      181 agaaatgcta attgttcaaa acttgaaccc ataggttgtg aaaaagtatc ttcttctcgt
      241 tgattttcat ctttggtaat acctggtaat acaagaacag aatctgctaa ttgaccaatc
      301 gacgagtctt ttttcatggt aactaaagca atgcttacac cactttgatt tgctttggcc
      361 gctaaatttt ttaaactacc tgtttcacct gagccagaac agataattaa taaatctcca
      421 ggttgcgaat gtggtgaact aatttcaccg acaaggctga ctgaaaaacc taaatgcatt
      481 aagcgattcg taaatgcttg catggcaatt ccagagcgtc ctgcaccagc tagaaagatg
      541 tgtttagctt gaacaatttt ctcactaaat tgttctaact ctacagaatt aatacgtttt
      601 gagttatttt gtaattctaa gataatcgct tctaaatagt ttttattttg gtctttattg
      661 gtcatgatct tttacttcac ttcctctaat gattgattta gtttatcaat ttgttcttgc
      721 ggggtatttt tcccattgaa gatgaaaccg cctgatacaa aaatatccgc ccctgcttta
      781 gaacaaactt taatcgtttg attatcaatc ttgccatcta cttcaatatc atacgtataa
      841 tcattttgtt tgcgtattac atctaactgt tcgattttaa ctactgtctc ttgaataaaa
      901 tgcttattct ctgttcctgg attgattgtc attactaata cttgatcgac taaagataat
      961 actggttcaa taaaactgac tggtgttgca ggattaaaag taatccctac ttttgctcct
     1021 gcttccttga ctaactgaat gaccttcatt gcgtgtttgg tagcttcaat gtggaaggta
     1081 acaatatctg ctcctgcttc tacaaatctt gtgacaattt tttctggtgt atcaatcatc
     1141 aaatgaacat caaacacaac gtctgttagt ggacgaatat ctttaataac acttggtcca
     1201 aaagccatat ctggtacaaa acagccatcc attacatcaa tatgcaaata atcaacattc
     1261 ttttctttta agatttcaaa taactcttta acttctgcga taggtgcatt taacaatgat
     1321 ggagcaattg tcattctcta ttcccccgtt tattctccca taatcatgag attgattgtt
     1381 ctagttcgct ctcttagttg atcccaatcg acaaaataga cacggcccat ttctcctaat
     1441 aaaagtttac cttcttgaac aattaaagtc attgagttgg ttccataaat agatgaacgt
     1501 aaatgtgcat cggtatttag catgacccat ttttctgcag ggtaattggg atcagataat
     1561 cttgttccaa aatcaatgtg ttttggcccc ggactgttgt attgattttc actcgtcatt
     1621 tttggaataa tcgtttccat tacattgttg atgtctacat gtaaataatc atcaccaaaa
     1681 aagtttgtgt catgcatcgt ttcttcaaaa aagagtgagc aagttgtatg cgggctcgaa
     1741 attactaaca ttccattctc aatacctgct gtatcaactg tatctaaaag ttcttttcga
     1801 atatcatgat acgaaggacg ccctgaaact gtttcaatag tcaaagtgtc atgatgaaaa
     1861 cgcatattaa acacctaatc tttcttttac atctgcataa atggcatctg taccaatacc
     1921 tgttaataag cccaagccat tcgttacttt agctttaatt acatcaggga caattgttgt
     1981 acttactaca gcatcatatt catcaatttt atttagctca tcagcaattt tagattgata
     2041 aattttcact tgatctgtta atccttcttc ttccaaccat tttgataatt ttgctactgc
     2101 aattgtagaa gtagcatgac ctgctccaca aataactaat aattttttca tattgatctc
     2161 ttctttctta ttttaatttt atgattaagc tttcttcact ttgttcatgt agatagaagc
     2221 acctactaaa acaactccta atccacctat tccaattaca ccaaagtaag atgctaataa
     2281 aacaaacaac cctactggcc ataatccaca taacaacata gttactaatc ctgctgtccc
     2341 cacatcgaaa cttgctaatg taaacacatc agtgataatt ggtgccaacc atgttgccat
     2401 atataatgtt gtaacaacat acaacgaaca tcctactaat gatctcacaa tatttccttt
     2461 aaaataagga atcattaaac agatggcgaa agcaaagaat gctaaatctc caaaaggtaa
     2521 tactttattt cctggaagaa ttagtgctag caataatgag attggtacaa gcaatagaga
     2581 agttgacata atagttggat gacccacagt taacgctgca tccattccaa tgtttacttg
     2641 gcgaccattt aactttttat tcgtaaattc tttcgccgct tctgcaattg gcattaaacc
     2701 ttccataaac attgctacca ttttaggcat aatcttcatc actgccgcag tcataattcc
     2761 taagtttcca attcctgcta cgtcatagcc tgccataata cccactacaa taccgataat
     2821 taggccaata acaactgtgt cacctaaaac accgaaacgt tttgttaatg tttctgcatc
     2881 tgcatctatt ttattaaatc caggaatacg atcaaaaacc caatttactg gtttggcaaa
     2941 tagtactcct gataatccca tcatatgtgt gatagagatt cctggatagc cgaaaaattc
     3001 gctaacagtt ttttgtgtac gatctgctaa gaaccattgt aaaattactg cgacaatcgc
     3061 gccaattaag ccaaaccaaa agtttcccgt aacagattga gtaatcatac ctaggaaaat
     3121 tgcgaaccaa aagttccaaa tatcaacatt caatgttttt gtccaaccca aaaccacaaa
     3181 tatgaagtta agaattaatg aaattggaat cattaaaatt cccaatgatg tagagtaagc
     3241 caaaggtcca ccaacaccta cgccaatatc taggatattc aggccaccac cgtattgttc
     3301 ccccatttgt tgaattgctg ttcctaaacc atctgataat aaacttaaaa ctaatcctaa
     3361 accaatagaa ccgataccaa cagtaacgcc ggaaacaaat gctttaccag gttttaatcc
     3421 aaataatatc ccgattacaa aaataatcag tggtaaaaag acgtttgacc ctaatccaat
     3481 aaaccattca aaggcattta atacaatatc catctctcta ttcctcctaa taaactcctg
     3541 cagaatgtac taactcaata aatgcttctt tcgtggtaca tgcaagtaat tcatttactt
     3601 tttcttcatt ttgaaatagg ctaactaagt tttgtagtgt atcaatttgt tcatgaggtt
     3661 gtgacattgc caacataaag actaaacgta caggtatttc atgagattta tctgtcatat
     3721 ctgaaaatac cagtggttcc tttaatgagg caaaacagat ttgagagcta tttacatgtg
     3781 cggaatctgt atgtgggata gcaaacccaa tttgatttac ttctaatcca gttggaaatt
     3841 cttgttcacg gttggtgata ccttctaaga aactatcttt cacgcaacca gcgtttttta
     3901 attgttcaga cattacttga aacaattctt tttgatcttt cacttctaat tgaaataaac
     3961 ataattcttt atctagatac atttttattc tcctttttgt tgaaaaattc ttagtaattt
     4021 ttcgctatca tgaacttgta acaagcgatc gaccatttct ttttgcccaa taaattggta
     4081 aagttcttca tatactcctc taatctcaga tacatcttct tcagctaaac taaccataat
     4141 gactaatttg acttttcgat cgccccaatt aatcgcattt tttaatgcta caataaaaat
     4201 gttggattca atgaccattg atggattcgc atgcggtaaa gcaacaccac tttctaaatt
     4261 agtattcccc attttctctc tagagaagat agaagcacga aactcttcag taactaatga
     4321 attggcttct agttcggaaa tcatcaaatc caaacattct tctttgcttt ccacctgtac
     4381 tcctgttttt actaaagatg attgaacaaa tttagatatg gtgggaactt taaccgcttt
     4441 aggaatcagt tcattttgaa taaatcgcga aggttcttct attaaatatt tagtataagc
     4501 ttccataata ttcatataat ctgttttact taccaatgga gataccctta ctaagggtat
     4561 accacctgca tcaaaggaaa gagaggaaat aatcaaatca acttccccaa tttcagctaa
     4621 atgaaactta tctaaagttg atatttctac attatctttt gctggtaaaa accgtcgaac
     4681 tttattgtaa attaattgcg cagaagaaat accgtattgg caaataatga tgatattatt
     4741 tgctttggac tgatcttcaa tagcaatctg aaaatgaatc aaaattaagg aaacttcatc
     4801 gtccgttaaa gtcacatcaa attcttcttc aaggatggac atggcgtacc atactatgct
     4861 aaagagttct gaatattgag atttgatatt ttctaataac ggattttgaa ttttaatacc
     4921 tttttttaaa cgaaggacca ttgctggcac atgatacaat aaggattgat ataatcgatc
     4981 attttgagtc aaatcaattt tttcaatctc actcattctt tcaatcaatt tatgaacgct
     5041 catagcgtac tcttcattgc tggtttttaa attatttgtc acacgatgtg caaatagttg
     5101 acgacataga tattctttat ctgcgtcata aaatggaaga ttcaattttg ccttcaattc
     5161 ttcaattaaa ccatttgcca caatgtacgt ttccatatag cgaatgctat tgaagaggaa
     5221 ttcttcttca acagttaaat gatgaccttt cagtaaacgg ttacattgga taactaaagt
     5281 agaaatcaat gaccgaacat agtaatcaga aaccttttca gtcagttcac tgtaatcatt
     5341 gaatagcaaa tcatgaatga cactaataac ttttgaatca aacaacatat ttaaaacttg
     5401 aaaaaatgtt tttgattcca tgtcattcgc ataatagaat acaagttgct tgattgctcg
     5461 ttgaattgct tgttcattac cagtaaaact aattccttct gcagatgact gcaacttaac
     5521 ttctccactg ccaataattt tattgataat tctcaggtca ttgtataacg atgttttact
     5581 aaccataaaa ctttgagaca aatgagttaa agaaacaggc ttatcaccaa acaaaacttc
     5641 tttaatgatc tcgacacgtc ttttttctgg agaataaata tctttttcaa gataattttc
     5701 taccgatgaa atcaaatgtt caacgttttt cctatcacct atgagtttga tacccaaaga
     5761 aggtaatcgc tcaattgtta cttggtaatt aactacataa tcttccaaca attcaatatc
     5821 ttgataaatg gtcttggtcg acactgccaa tttatcacta aaaaaagaag cgggtttaaa
     5881 gtcttcttgt cgacttaata atttt
//
LOCUS       DASHWH010000111         5876 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.117, whole genome shotgun sequence.
ACCESSION   DASHWH010000111 DASHWH010000000
VERSION     DASHWH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.117"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            640..1374
                     /locus_tag="U2R41_002663"
     CDS             640..1374
                     /locus_tag="U2R41_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8890002.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="U2R41_002664"
     CDS             1390..2559
                     /locus_tag="U2R41_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8890003.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="U2R41_002665"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="U2R41_002665"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM8890004.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="U2R41_002666"
     CDS             3649..4575
                     /locus_tag="U2R41_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEM8890005.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="U2R41_002667"
     CDS             4594..5844
                     /locus_tag="U2R41_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8890006.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1203 g         1751 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacagca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag ttaagt
//
LOCUS       DASHWH010000112         5856 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.118, whole genome shotgun sequence.
ACCESSION   DASHWH010000112 DASHWH010000000
VERSION     DASHWH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.118"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            127..579
                     /locus_tag="U2R41_002668"
     CDS             127..579
                     /locus_tag="U2R41_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890007.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="U2R41_002669"
     CDS             593..1282
                     /locus_tag="U2R41_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890008.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="U2R41_002670"
     CDS             1407..3758
                     /locus_tag="U2R41_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEM8890009.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="U2R41_002671"
     CDS             3838..4233
                     /locus_tag="U2R41_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890010.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="U2R41_002672"
     CDS             4558..5175
                     /locus_tag="U2R41_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8890011.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="U2R41_002673"
     CDS             5216..5515
                     /locus_tag="U2R41_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEM8890012.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(5704..>5856)
                     /locus_tag="U2R41_002674"
     CDS             complement(5704..>5856)
                     /locus_tag="U2R41_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8890013.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
BASE COUNT         2028 a          793 c         1183 g         1852 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagg gttctgttgc gaagtttgaa aatcaaacgc gccctctctg aactccaaat
     5701 atcttaggct gttattccca ttaatacctt gatttcagta gacaccgaaa agccgaagag
     5761 cgttccattt cttcggttct ttttatatat tcctcgaatg gtctccatgc ccttaatcgt
     5821 ggaagaggct gtacggagac tttgataaaa tttatt
//
LOCUS       DASHWH010000113         5725 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.119, whole genome shotgun sequence.
ACCESSION   DASHWH010000113 DASHWH010000000
VERSION     DASHWH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.119"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..482)
                     /locus_tag="U2R41_002675"
     CDS             complement(<1..482)
                     /locus_tag="U2R41_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEM8890014.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     "
     gene            589..1446
                     /locus_tag="U2R41_002676"
     CDS             589..1446
                     /locus_tag="U2R41_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890015.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(1527..1862)
                     /locus_tag="U2R41_002677"
     CDS             complement(1527..1862)
                     /locus_tag="U2R41_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890016.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(1929..3299)
                     /locus_tag="U2R41_002678"
     CDS             complement(1929..3299)
                     /locus_tag="U2R41_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEM8890017.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(3377..3913)
                     /locus_tag="U2R41_002679"
     CDS             complement(3377..3913)
                     /locus_tag="U2R41_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8890018.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            4222..5703
                     /locus_tag="U2R41_002680"
     CDS             4222..5703
                     /locus_tag="U2R41_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890019.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTFFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         1819 a         1161 c          853 g         1892 t
ORIGIN      
        1 ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct
       61 tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc
      121 atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca
      181 cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca
      241 gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct
      301 gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt
      361 ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct
      421 tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc
      481 aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg
      541 taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat
      601 aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat
      661 atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa
      721 caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca
      781 tggtatcgct atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc
      841 cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg
      901 gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc
      961 gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta
     1021 gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc
     1081 gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt
     1141 tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat
     1201 actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg
     1261 gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca
     1321 ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt
     1381 ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat
     1441 aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat
     1501 gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg
     1561 tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt
     1621 cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca
     1681 tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact
     1741 ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct
     1801 gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc
     1861 ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac
     1921 taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact
     1981 tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca
     2041 tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag
     2101 caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga
     2161 ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa
     2221 ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct
     2281 tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc
     2341 actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc
     2401 agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt
     2461 tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga
     2521 atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct
     2581 tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt
     2641 gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac
     2701 tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc
     2761 acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc
     2821 actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc
     2881 ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt
     2941 gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca
     3001 gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg
     3061 aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat
     3121 tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga
     3181 aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct
     3241 aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt
     3301 ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac
     3361 tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg
     3421 tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat
     3481 ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga
     3541 tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta
     3601 aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata
     3661 agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa
     3721 cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa
     3781 tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc
     3841 catattcttc cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta
     3901 ctctccccat cattcttacc tccgtctctt ttcatctacc cataaataaa taacataaat
     3961 aattctaaaa atattattga taattatcaa aacatattta gttatatcca ttaaaaatca
     4021 atttttccgc cttaaaaaca agaatatctt gacaaaatac cctaggaaat aaacaataga
     4081 ataaacaagg taacaaaaaa attaaataac gttatataaa aaagaaggcg acactctgtg
     4141 agaataacac agactgaaat gactttccac tcacagtttt tgtctaacta tatacaaaaa
     4201 cagaataaaa aggaggtatt tatgctaatt gaagcaagaa aaaaagagtt acttgttcta
     4261 caaatggtca ttcaaaaaaa taattgttct ttacaagaat tatccaatga cttacatatt
     4321 ccaaaacgga caatcaaaga actgatccga aaaataaatg tatcactcaa gcaattttta
     4381 ttgattgatg aatttattta ttctaatcat aaaggagaga tccaaaccaa agaaaccgat
     4441 caaactaaaa agttagctgt tttttctaag ttaaaattat gctacctaaa agaatccaat
     4501 cgttttaact atttgatgtt actaatcaat tatccacaaa ctgctgtacc aaaaaaatat
     4561 ttacttgaac aactttatat atcgccctcc tatctcgaaa aactgacaaa acagctcaat
     4621 cattccttaa aaaaattcca tttacggatc ctatctgtaa aaaattgctg tactcttgaa
     4681 ggaaatgagt tatctatccg tctctatctt tacttttttc tttcagatac atttggaggt
     4741 aacgattggc cattttcctc acttgaatta aatgcactaa aaatgaacag tacagatgag
     4801 caaatagaaa aaatttcaaa acaagaaaaa aatatctact tgctgttaac tttattcata
     4861 ttacgaaaca aacaacaggc atattttcca aaaacttctt ctgaaatcac tgaattgatg
     4921 gacttattgc agcatacaca tgattattca gaatacttta cttctttttc agtgagtcgc
     4981 ccttgtgaag agacagaaac agagagagcc tactttaatt attttttcta ttgttttttt
     5041 ccaaatcaaa tctcttttcg acaaaaacag gagattggtt ttttattttc cacaagcaaa
     5101 aaccttattt gtcgacaagt cggtcaattg atgacttact taactcaaca gtttcctctc
     5161 aaccatttag aaaataaaat accaatttat ttttattttc ttgtgttggc agttgttttt
     5221 ttcgaactgt tgggtgaatc cagcagtagt ttttctacac tttgctttcc agaagccacg
     5281 tccaatcatt ctagttcttt tatacaggaa aagaaattaa gagaaaaaat cgatacattt
     5341 ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc ttagtcaatt gatccaacac
     5401 ttactacaaa cagaaacgaa agagcagtta ctcatccatt tgcagataac caaacagttt
     5461 acaggaaatt atcatcttca aagccatttg cagcagttat ttcgcaaaga tgctgttaaa
     5521 attaccgcat attcagaaca tgctgatttg atcattacag atcagcatga gaaaatttca
     5581 gatgaaacgg agactttcta tctggataca acgcacaata cagtgttatt tggcaagtta
     5641 ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg agaacaaaac aaagaggatg
     5701 tgacaaaatt cttgtcacat cctct
//
LOCUS       DASHWH010000114         5664 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.120, whole genome shotgun sequence.
ACCESSION   DASHWH010000114 DASHWH010000000
VERSION     DASHWH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.120"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..193)
                     /locus_tag="U2R41_002681"
     CDS             complement(<1..193)
                     /locus_tag="U2R41_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890020.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(736..2886)
                     /locus_tag="U2R41_002682"
                     /pseudo
     CDS             complement(736..2886)
                     /locus_tag="U2R41_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3032..3607
                     /locus_tag="U2R41_002683"
     CDS             3032..3607
                     /locus_tag="U2R41_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8890021.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3821..4516
                     /gene="vanR"
                     /locus_tag="U2R41_002684"
     CDS             3821..4516
                     /gene="vanR"
                     /locus_tag="U2R41_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM8890022.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4494..5648
                     /locus_tag="U2R41_002685"
     CDS             4494..5648
                     /locus_tag="U2R41_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM8890023.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1729 t
ORIGIN      
        1 gttcaaaatc taaaatcaac acatcatggg gagtgtgata ataaatattt tcccttaatg
       61 taccaacaat ggtaattcgg tcaattgacc attgcatttt taagggggtg cacatagttt
      121 gtgcatttaa ccgcctgtta gaaatacggt taagtttttc ccactcattt acgattatgc
      181 cttttttact caaaaatagc tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc
      241 agagttcacg taactctaaa aatccacatc caaaaatctc tagcttgaag aaacctaaaa
      301 atattcgtat aatgaacttg agttacggaa ctaggtgaag tagtttccgt aaaggtcgag
      361 agtgcgtcaa cactgctcga ctattttttt actctttttt ctcattttct ttgattatag
      421 cactcctcta gaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc
      481 tcctcctgcc gaaatagaat ggcgctgcca gcatttattt cgttcagcta tcggctacac
      541 aatctaacac gaccgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac
      601 gtgtttcacc gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca
      661 aaatatgcca ttttagagct attgtagaaa atatagtttg aaatgcaatt ttcttctttc
      721 agctttttat ttcccacttg taaatcggta gatggtggct ccttttccag ttccataatg
      781 tggatttgca tctgcatgta gtgatgaaac acctagctgc attctcatac catctgacga
      841 agatgttgta ccgtcgcccc aatagaaagg caattgtaat ttatgatgaa agtttactaa
      901 tatggcttgg gctttattca tggcatcttc atacatgcgc cattgagata cattggctag
      961 ttgcttatat gtaagtccgg gtgtggcttc ggccatcttg ctcaagccaa tattcattcc
     1021 cattcctaaa agggcagcca tgataatgat tgtttcttcc ttatctggtt ttcgattatt
     1081 ggaagcatga gtgaattgct catgaaatcc tgttatatgg gccacatcca tgagtaaatc
     1141 agttaatttt attcttggta gcatctgata aaggcttgca ctaaattttt ttgcttcttc
     1201 tggaacatct ttttctaagc gtgcaagtga tagctttcct ttttcaagag aaaccccatc
     1261 taacttattg gaattggcag ctaaccactt taacctttca ttaaagctgc tggttctctc
     1321 cgttatataa tcttcgaatg ataaactaac tgataatctc gtattcccct tcgattgatt
     1381 ccatgtatct tccgaaaaca aatattcctc aaaatcccta tattgtctgc tgccaacaat
     1441 ggaaacatct cctgcccgaa catgctcccg aagttctgtt aaaacagcca tttcatagta
     1501 atgacgatta attgttgtac catcatcctc gtataaatgt cttttccatc gttttgaaat
     1561 aaaatccaca ggtgagtcat caggcacttt tcgctttcca gattcgttca ttcctcggat
     1621 aatctcaaca gcttgtaaaa gtggctcatt tgcctttgta gaatgaaatt ccaatactct
     1681 taatagcgtt ggcgtatatt ttcttagtga ataaaaccgt ttttgcagta agtctaaata
     1741 atcatagtcg gcaggacgtg caagttcctg agcctcttct actgaagaga caaaggtatt
     1801 ccattcaata accgattcta aaaccttaaa aacgtctaat ttttcctctc ttgctttaat
     1861 taatgcttgt ccgatgttcg taaagtgtat aactttctca tttagctttt taccgttttg
     1921 tttctggatt tcctcttgag ccttacgacc ttttgataac aaactaagta tttgcctatc
     1981 atgaatttca aacgctttat ccgttagctc ctgagtaagt tgtaataaat agatggttaa
     2041 tatcgaataa cgtttatttt cttgaaagtc acggaatgca tacggctcgt atcttgagcc
     2101 taagcgagac agctgcaaca ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa
     2161 atccattcct cgtatgtatt cgagtcgttc tattattttt agaaaagttt cgggtgaagg
     2221 atgacccggt ggctctttta accaacccaa tatcgtttta ttggattcgg atggatgctg
     2281 cgaggtaata atcccttcaa gcttttcttt ttgctcattt gttagagatt tactaaccgt
     2341 attaaatagc ttcttttcag ccattgccct tgcttcccac accattcttt caagtgtagt
     2401 gatagcaggc agtataattt tgttttttct tagaaaatct atgcattcat gcagtagatg
     2461 aatggcatca ccattttcca aagctaattg atgaaggtac ttaaatgtca ttcgatattc
     2521 actcagggta aaagttacaa agtcgtattc acttcgaatt tctttcaaat gatcccaaag
     2581 tgtattttcc ctttgaggat aatgatcaag cgaggatgga ctaacaccaa tctgtttcga
     2641 tatatattgt atgaccgaat ctgggatgct tttgatatga gtgtatggcc aaccgggata
     2701 ccgaagaaca gctaattgaa cagcaaatcc taaacggttt tcttccctcc ttcgcttatt
     2761 aactatttct aaatcccgtt tggaaaaagt gaagtaggtc cccagtatcc attcatcttc
     2821 agggatttgc ataaaagcct gtctctgttc cggtgtaagc aattctctac ctctcgcaat
     2881 tttcattcag tatcattcca tttctgtatt ttcaatttat tagttcaatt atatatcaat
     2941 agagtgtact ctattgatac aaatgtagta gactgataaa atcatagtta agagcgtctc
     3001 ataagacttg tctcaaaaat gaggtgatat tttgcggaaa atcggttata ttcgtgtcag
     3061 ttcgactaac cagaatcctt caagacaatt tcagcagttg aacgagatcg gaatggatat
     3121 tatatatgaa gagaaagttt caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt
     3181 agacgattta caggaagatg acatcattta tgttacagac ttaactcgaa tcactcgtag
     3241 tacacaagat ctatttgaat taatcgataa catacgagat aaaaaggcaa gtttaaaatc
     3301 actaaaagat acatggcttg atttatcaga agataatcca tacagccaat tcttaattac
     3361 tgtaatggct ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg
     3421 gattgaattg gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa
     3481 tcacgcagga atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca
     3541 aatttgtgaa attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa
     3601 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
     3661 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
     3721 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
     3781 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
     3841 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
     3901 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
     3961 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
     4021 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
     4081 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
     4141 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
     4201 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
     4261 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
     4321 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
     4381 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
     4441 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
     4501 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
     4561 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     4621 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     4681 acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg
     4741 cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact
     4801 ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc
     4861 tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa
     4921 agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg
     4981 cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg
     5041 ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt
     5101 atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga
     5161 taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat
     5221 tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga
     5281 ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg
     5341 ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg
     5401 tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg
     5461 aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat
     5521 tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg
     5581 ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga
     5641 ggtcctaaga gatgtatata attt
//
LOCUS       DASHWH010000115         5463 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.121, whole genome shotgun sequence.
ACCESSION   DASHWH010000115 DASHWH010000000
VERSION     DASHWH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.121"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..933
                     /locus_tag="U2R41_002686"
     CDS             <1..933
                     /locus_tag="U2R41_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890024.1"
                     /translation="RWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLA
                     IDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHI
                     ESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAM
                     LIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVT
                     QNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCL
                     ISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3119
                     /locus_tag="U2R41_002687"
     CDS             936..3119
                     /locus_tag="U2R41_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8890025.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3116..4117
                     /locus_tag="U2R41_002688"
     CDS             3116..4117
                     /locus_tag="U2R41_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8890026.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4132..5046
                     /locus_tag="U2R41_002689"
     CDS             4132..5046
                     /locus_tag="U2R41_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8890027.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5291..5407
                     /locus_tag="U2R41_002690"
     CDS             5291..5407
                     /locus_tag="U2R41_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM8890028.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1048 c         1154 g         1534 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcaa tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtaatta tgaaaaatac caaagattct
      121 gaatcactgg ctattgatat tttgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt gcgagggttg
      241 atgaaagtga ttgaagaatt acgggttgag aatacgccac taagtaccag catagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcccatctgc tttttagtga tggttatgta
      361 gagcagtcta tcagccttga aaaacaactg aacattatac aggttgccga cttggtactt
      421 cctgacaagg aaacttcctt tgaggaatat accacaatgg agttactatc ggtagcaatg
      481 ctgattgtca ttagtacctt tgcgttggac tttatccata cagaccgaag tattttcaag
      541 attgtagact tagacgaagc atggagcttc ttacaggtgg cacaagggaa aacactatct
      601 atgaagctgg ttcgtgctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagatgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt
      721 tccactgacc ttaacgagat taaaaaaacc cttgcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata ttaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggctaccgtg gaactggtcg gacggaattg
     1201 gaaaacaagt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtcttt
     1261 atgtttccaa tgcgacgggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggc aagaatatgc agacccttgc aggggttacg cctagtggac
     1381 tatcaacaga gggtttctat gttggattcc ttttactctt gattttggtt cttggggttt
     1441 atgttgccta tacaggactg ataaagagag aaaccacaaa ggcaattcat gccctcatga
     1501 attttgtgct ggtttttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gcaacgctag tttgtcactg ggtacaaaaa
     1621 ttgtcatgcc ccattcagat agtcaaggca aggacagtgt ggacttgata cgtgacagcc
     1681 tattttccat acaggttcag caaccgtggc tactgcttca atataacagt tcagacattg
     1741 aaagtattgg tattgaccgt gtggaaagcc tgctctcaac cagtccagat tccaacaatg
     1801 gggaagacag agaaaaaatt gttgcggaag aaattgaaga tagaagcaat accaatatga
     1861 cgattacaaa gacgattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tactttaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtatgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgctc cgtgactgct ttaggggctg ggtctgccat tgccagtgct acgggtaaaa
     2401 aaggacagtc gggttcggga agttctgcga gtacacaagc agatcactct cgaccaaacg
     2461 ggcaggaaaa atcaacacta ggaaagcgta tcggtcaaac catcggtaca gtagctgata
     2521 ccaaagacag aatggtagat actgctggta atttgaaaga acaggttaag gatttgccga
     2581 ccaatgcaag atatgcagta tatcaaggaa aatctaaagt gacggataat gtccatgatt
     2641 taaccagaag tatttctcaa accaaagcgg ataaagccag tggacgcaag aaacagcagg
     2701 aacaaagacg gaaaaccatt gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa
     2761 aacagccagc ttcttccgtt catgaaagac cagccactaa gcaagaacaa tctcatgatg
     2821 gacagacttc aaggcaaaca actgtacagg cttcctatag ggaatctcaa caagccaaac
     2881 aagagcgtcc aacagttaag tccgattctt caagtttaaa aacggaacgt caaagtaata
     2941 cagttcaaga aagaacggtt caaaagccag taacttcaac tgcaccaaca gatagagctt
     3001 cacaacgttc aatcacaaaa gaacgtcagt ctactgttca aagagtacca ctacaaaata
     3061 caaaaagtag accaccaatc aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa
     3121 gctgaaaact ttagtgattg gtggttcggg attattcttg atggtcttct cactgcttct
     3181 gtttgtcgcc attttatttt cagatgaaca agatggcggt ttttccaaca ttcactatgg
     3241 cggtgtggat gtttccgcag aagtgctggc tcataagcct atggtagaaa aagttgccaa
     3301 agaatatgga attgaagaat acgtcaatat acttcttgcg attatacagg tggaatcggg
     3361 tggtacagca gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt
     3421 gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgagttat tagccagtag
     3481 cgaaaggctt ggtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt
     3541 cttaggatat gtggctaatc gtgggaataa atataccttt gaactggctc aaagtttctc
     3601 aaaagagtat tcgggtgacg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa
     3661 tgggggctgg cgatacaatt acgggaatat gttttatgtg caactggtaa cgcagtatct
     3721 tgtcacaaca gagtttgatg atgatacagt acaagccatc atggacgaag ctctgaaatt
     3781 tgaaggctgg aagtatgtct atggcggagc ttccccgact acctcttttg attgtagcgg
     3841 actgacacaa tggacgtatg gaaaagctgg aatcaactta ccacgaacag cacaacagca
     3901 atatgatgta acccggcata tcccattatt ggaagcaaaa gccggcgatt tggttttctt
     3961 tcattctacc tataacgctg gctcttatat tactcatatc gggatttatt taggcgataa
     4021 ccgtatgttt catgcaggcg accctatcgg ttatgccgac ttaacaagct cttactggca
     4081 acagcattta gtgggagcag gacgaatcaa acaataagaa aggacgattc aatgatgaaa
     4141 ttcggaaaaa atcagaatac agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat
     4201 aaggtcaatc ctcgtaaaaa ggttgtgatt gtcctatggg tacttttagg acttagtttc
     4261 agctttgcga tattcaagca ctttacagcc attgataccc ataccattca tgaaaccacc
     4321 atcatagaaa aggaatatgt cgatactcat catgtggaaa attttgtaga gaactttgcg
     4381 aaagtctact attcatggga actaaacgac aagtccattg ataatcgcat agaaaacctg
     4441 aaagcatatc tgacagagga acttcaagct ctgaatgtcg atacagttcg taaagatatt
     4501 cctgtatcgt cttctgtaaa aggatttcag atatggacgg tagaagcaga tggcgacaat
     4561 caatttgatg taacctacag tgtagaccaa ctcattacag agggggaaaa tacaaagacc
     4621 gtccactctg cttatatagt gagtgtctat gtagacagta ctggaaatat ggtcctcatt
     4681 aagaatccga ccattactag cataccaaag aaatcagact ataaaccaaa agctattgaa
     4741 agtgagggta cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
     4801 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
     4861 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat ttacaatcgt
     4921 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
     4981 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     5041 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     5101 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     5161 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     5221 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgcacccag tttaagaata
     5281 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     5341 tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg cccttttggg
     5401 cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     5461 tgc
//
LOCUS       DASHWH010000116         5433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.122, whole genome shotgun sequence.
ACCESSION   DASHWH010000116 DASHWH010000000
VERSION     DASHWH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.122"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..177
                     /locus_tag="U2R41_002691"
     CDS             <1..177
                     /locus_tag="U2R41_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890029.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="U2R41_002692"
     CDS             190..690
                     /locus_tag="U2R41_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8890030.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="U2R41_002693"
     CDS             787..2634
                     /locus_tag="U2R41_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEM8890031.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="U2R41_002694"
     CDS             2637..3533
                     /locus_tag="U2R41_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8890032.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="U2R41_002695"
     CDS             3583..3972
                     /locus_tag="U2R41_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8890033.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5433
                     /locus_tag="U2R41_002696"
     CDS             3959..>5433
                     /locus_tag="U2R41_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890034.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1856 a          784 c         1152 g         1641 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DASHWH010000117         5345 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.123, whole genome shotgun sequence.
ACCESSION   DASHWH010000117 DASHWH010000000
VERSION     DASHWH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.123"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            37..939
                     /locus_tag="U2R41_002697"
     CDS             37..939
                     /locus_tag="U2R41_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890035.1"
                     /translation="MNIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISS
                     RDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLL
                     FSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFI
                     HSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMK
                     NNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQ
                     FHPIFEELLHAFDTRPPVRNEVEE"
     gene            936..2963
                     /locus_tag="U2R41_002698"
     CDS             936..2963
                     /locus_tag="U2R41_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890036.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2960..3982
                     /locus_tag="U2R41_002699"
     CDS             2960..3982
                     /locus_tag="U2R41_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8890037.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3999..4928
                     /locus_tag="U2R41_002700"
     CDS             3999..4928
                     /locus_tag="U2R41_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8890038.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            5173..5289
                     /locus_tag="U2R41_002701"
     CDS             5173..5289
                     /locus_tag="U2R41_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM8890039.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1755 a          948 c         1091 g         1551 t
ORIGIN      
        1 gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt
       61 gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca
      121 gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag
      181 aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg
      241 ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac
      301 catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc
      361 acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta
      421 cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa
      541 attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct
      601 atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac
      661 gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt
      721 tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat
      781 gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc
      841 tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt
      901 gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc
      961 ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc
     1021 tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat
     1081 actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct
     1141 ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca
     1201 ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga
     1261 aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc
     1321 agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc
     1381 tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag
     1441 aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt
     1501 cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca
     1561 gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga
     1621 aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc
     1681 tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt
     1741 tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag
     1801 aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa
     1861 cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag
     1921 gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt
     1981 tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat
     2041 ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt
     2101 ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa
     2161 ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac
     2221 aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga
     2281 atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt
     2341 caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc
     2401 gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac
     2461 aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc
     2521 gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca
     2581 aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat
     2641 ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac
     2701 gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag
     2761 aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg
     2821 tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag
     2881 agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg
     2941 ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc
     3001 tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg
     3061 cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt
     3121 ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc
     3181 ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag
     3241 ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg
     3301 ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc
     3361 tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa
     3421 aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc
     3481 agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa
     3541 tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga
     3601 tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt
     3661 ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta
     3721 tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga
     3781 aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc
     3841 aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg
     3901 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     3961 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     4021 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     4081 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     4141 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     4201 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     4261 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     4321 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     4381 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     4441 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     4501 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     4561 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     4621 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     4681 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     4741 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     4801 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     4861 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     4921 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tctttttgat
     4981 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt
     5041 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg
     5101 gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     5161 tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg
     5221 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     5281 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     5341 gatgc
//
LOCUS       DASHWH010000118         5341 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.124, whole genome shotgun sequence.
ACCESSION   DASHWH010000118 DASHWH010000000
VERSION     DASHWH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.124"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(62..280)
                     /locus_tag="U2R41_002702"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="U2R41_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="U2R41_002703"
     CDS             complement(546..740)
                     /locus_tag="U2R41_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890040.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="U2R41_002704"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="U2R41_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="U2R41_002705"
     CDS             2092..3132
                     /locus_tag="U2R41_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8890041.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="U2R41_002706"
     CDS             3956..4288
                     /locus_tag="U2R41_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM8890042.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="U2R41_002707"
     CDS             4907..5248
                     /locus_tag="U2R41_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEM8890043.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DASHWH010000119         5305 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.125, whole genome shotgun sequence.
ACCESSION   DASHWH010000119 DASHWH010000000
VERSION     DASHWH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.125"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            93..1970
                     /gene="tdc"
                     /locus_tag="U2R41_002708"
     CDS             93..1970
                     /gene="tdc"
                     /locus_tag="U2R41_002708"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM8890044.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2213..3634
                     /locus_tag="U2R41_002709"
     CDS             2213..3634
                     /locus_tag="U2R41_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8890045.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3743..5110
                     /gene="nhaC"
                     /locus_tag="U2R41_002710"
     CDS             3743..5110
                     /gene="nhaC"
                     /locus_tag="U2R41_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM8890046.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         1586 a          973 c         1134 g         1612 t
ORIGIN      
        1 aaaatcaggc caaaacttag tttacagaat agaagaacag gtgtaatgta gggctcgaca
       61 aaatttaaat atttttttag gagggtttca ctatgagtga atcattgtcg aaagatctaa
      121 acctaaatgc cctattcatt ggggacaaag ctgagaacgg ccaaatctat aaagctttgt
      181 tgaatgaatt ggtggatgaa catttaggct ggcgtcaaaa ctacatgcct caagacatgc
      241 ctattattac gccagaagaa aaaagcagtg ctagttttga acacactgtg aataaaacaa
      301 aggatgttct atcagaaatt tcagcgcgga tgcgtaccca ttccgttcct tggcataatg
      361 ccggacgtta ctggggtcat atgaactctg aaacattgat gccatcttta ctagcgtaca
      421 attttgctat gctatggaat ggtaataacg ttgcctatga atcttctcct gcaacaagcc
      481 aaatggaaga agaagtagga atggaatttg caaaattaat gagctataaa gatggctggg
      541 gccacatcgt tgctgatggt tctttagcaa acttagaagg actttggtat gcccgcaaca
      601 tcaaatcatt gccgcttgca atgaaagaag tgacacctga actagttgct ggaaaaagcg
      661 attgggaatt aatgaacttg tcaacagaag aaatcatgaa tctgttagac agcgtcccag
      721 aaaaaatcga cgaaatcaaa gctcactcag cacgtagcgg aaaacattta gaaaaattag
      781 gaaaatggtt ggttccacaa actaaacact attcatggct aaaagctgcc gacatcatcg
      841 gtatcggttt agaccaagtg attccagtgc cagttgacca caactatcgc atggacatca
      901 acgaacttga aaaaatcgtt cgtggtctag cagctgaaaa aacaccaatc ctaggtgtag
      961 tcggtgttgt aggatcaaca gaagaaggag caatcgacgg tatcgataaa atcgttgcat
     1021 tgcgtcgcgt attagaaaaa gacggtattt acttctactt acacgtagat gctgcttatg
     1081 gcggatacgg ccgtgcgatt ttcttagacg aagacaacaa cttcattcca tttgaagatt
     1141 taaaagatgt tcattacaaa tacaatgtct ttacagaaaa caaagattat atcttagaag
     1201 aagtacacag tgcatataaa gcaatcgaag aagcagaatc tgtaacgatt gacccacata
     1261 aaatgggcta tgttccatat tctgccggtg gtatcgtcat caaagatatc cgcatgagag
     1321 acgttatctc ttactttgcg acatatgtat ttgaaaaagg cgcagatatc ccagcattac
     1381 ttggggctta tatcttggaa ggttcaaaag caggggcaac agcagccagc gtttgggcag
     1441 cacaccatgt cttacctttg aatgtcacag gatatggtaa attaatgggt gcatcaatcg
     1501 aaggtgcaca tcgtttctac aacttcttga atgacttgtc atttaaagta ggagacaaag
     1561 aaatcgaagt tcatccatta acttatccag atttcaatat ggtagactat gtattcaaag
     1621 aaaaaggcaa cgatgactta gttgcaatga ataaattgaa ccatgacgta tatgattatt
     1681 cttcatacgt gaaaggaagc atttacggta atgagttctt aacttcacat actgactttg
     1741 ctatcccaga ctatggcaac agcccattac aatttgtgaa ccaactagga ttctcagatg
     1801 aagaatggaa ccgtgccgga aaagtaactg tattgcgtgc cagcgtaatg acaccataca
     1861 tgaacaaaga agaacacttt gaagaatatg ctgaaaaaat caaagcagct cttcaagaaa
     1921 aattggaaaa aatctacgca gaccaattat tggcaagcga agcaaaataa gcaaggtaat
     1981 catgttagaa aacagcttga atactaactg tttctgacgc atgtttggat ctgaagagca
     2041 aggggagacc taagctcttc gatccctttg ttttgaggtt gtctttgaag aaacccttca
     2101 agcaataaac tcaaagaatc ccctcaaata ttcgctgaat tgtttcgttt attgctgaaa
     2161 ctacttcaaa gacaccgtta ttttatccaa aaaatcaagg agagtgtcaa cggtgagtaa
     2221 atctgaagta agtaaaatgg gtcttgggac ctttatcggt ctgacaatgg cactgtgtgc
     2281 aacggttcgg agtattccta cgttagccgc agtcggttgg acattgattt tttattcaat
     2341 ttttgcagtt ttgttttttg ccggtccgat atcaatggtt tccggtgagc tttccacgat
     2401 gcttcctcaa gaaggcggtc ctcagttatg ggtaaaaaca gcactgggca gcaaatgggg
     2461 atttgtcgtt gcatggctat tatgggtgca aatgttccca ggtatggtaa tggtcgcttc
     2521 tactttagga ccattgttag gaaacacatt tggtaatgta gaacttggca ataatcactg
     2581 gttcgtttta ggttgtattt tagttatcta ctggatcatc acgatcttga acttgaaatt
     2641 cgatatggca aaagtcggcg ggaacatcgg tgtatggcta ggggtatata tcccagtagt
     2701 catcatgttc gttttaggtg ttttggcagc cttcaaagtc ggactagtct caaacggcta
     2761 tctaggtgac ttttcatggt caaaagcttt ccctgacttg gaacatattg attcattgaa
     2821 atacttagct ggtatcacat ttatttttgt tgggatcgaa atgagttctg tttacatgcc
     2881 tcgtttgaaa gacgcaacaa aaaattatac aaaaggcgta tttatcgctt tgatcggatt
     2941 agttttattg aatgtcatca atgcgatgct tgtagcaaat gtcgtaccag atggaaaaat
     3001 ggaattagca aatatcacgc agcctatctt gattgactgt caaatcctag gcttaccaga
     3061 agtgatcgga aatatcttca gcttcatggt atttattggt gtgcttttac aattatcagc
     3121 ttgggttact ggaccatcaa aaacaatcat ccaagtagct cgtgaaggat tccttccacc
     3181 aaaattcgga ttccataaag aaaacaaata cggcgtatct agaaatgtcg ttctaacaca
     3241 atcaatcgtt atttcattat ttgctttgct ttatggtgta atggatgacg taagtgctgt
     3301 attcttgaca cttacaaatg ctacaactgt tatttattgt atcgtgtaca ttttgattgc
     3361 tgtttcattg cttaaaatgc gtaagaaaca tccagaattc gaacgtcctt accgaattgg
     3421 taaaaacgga aacggattag cttgggtagt cagctgcatg ctgatcttct ctatcatcgt
     3481 tgtcgtattt gctacattag gtacagcgac attgagtgat gcattattag tagcagctat
     3541 cacagtggtc atgttcgtta ttccattgat catcaatcac ttcaagaaag attcttgggg
     3601 gattgaggta gaaaaaagtt tagaagaaaa ataaaaaaat aagtctttca gccgacgttt
     3661 tgtcgctgat ttagtaggag gtcctgccaa gtgaatagtt ttttcatggc agggcctttt
     3721 catttgaaat aggagatcga cgatgaataa aaatatttct ttaaaagaaa gtatcgctgt
     3781 attgatcgtg ttgttggcac tcctaggagt actgatcatc ggattccagc tctcgccaca
     3841 tatcccgatc cttgcagctt tcatgtgttt gttgttttat ggcagattta aaaagttcac
     3901 atgggatgag atccatgacg ggatcatcaa aggaatcact ccagggatca ttccaatcct
     3961 gatttttctt ttgattggtg tcttagttgc ttcatggatt ttatctggaa ccatccctac
     4021 gattatggtt tatggattta aacttgtttc tgtgcgtttc tttttaccga cagcatttct
     4081 tgtttgtgca attgtcggag tgactgtcgg cagttcattt acaacgattt cgacgattgg
     4141 aattgctttt ttggggatcg gtcatttact tggattcaat gatgcaatga ctacaggcgc
     4201 agtcgtttcc ggtgcctttt tagggaataa ctgctcgcct ttatcagata cgaccaactt
     4261 ggcagcgggt attggaggag ttaatctatt cgaacatatc gctggtatgc gttatacgga
     4321 tttcccagca ttcgtccttt cgttgatttt ttatacgata ttaggacata acagtcattc
     4381 agccgatcta gctagtatca atgaaatgat ccaaaacatg aaagcgagct tttggatctc
     4441 tccttggaca ttgattccac tagttatttt atttgggggg gcaatcaaaa aaattccggc
     4501 tatccctaca ttactagcgg gatcgacagc tgctctcgtt ctttctttta tccatgagaa
     4561 aagtttgacg gtcggaaaag cagcagacat cttgatgaat ggttatgtcg caaatacacc
     4621 tgatccaaag ctagatgaac tgctttctcg aggcgggatc atgagtatgt taggttctgc
     4681 tagtttgatt ttacttgcat tggcattagg tgggttactg atcaaatatc tgatcgttga
     4741 taccatcgtt caagaattaa aagaaaagat ggaccgacct tctcgtttag tcgggtttac
     4801 tgccttgagc tgtatcggaa tcaatctgat cgttggagaa cagtatctgt ccatcattct
     4861 tccaggagaa acattcaagc gttcttttga tgacgttggc ttatcaaaaa cctatttgac
     4921 acgaacacta gcggattcag gtgcgaccgt caattctctc gtaccgtggg gagtcagcgg
     4981 cacattcatt atgggaacga tgaaagttag cgcgttgcat tacttgccat tcgccttttt
     5041 ctcgattctt gcacctgtct ttacaatatt aggcggtttt ttattgaatc gtcgacgtgt
     5101 aaaaaattga aggaaggaaa aaagaggctg ggacagaagc gtttaactcc aagaaataag
     5161 aagaaactca agaaaattgc tttttaaatt tttgtgaatt tcagcttatt tccgaaggag
     5221 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
     5281 ttgtcccacg ctctttttta cagat
//
LOCUS       DASHWH010000120         5117 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.126, whole genome shotgun sequence.
ACCESSION   DASHWH010000120 DASHWH010000000
VERSION     DASHWH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.126"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(204..1166)
                     /locus_tag="U2R41_002711"
     CDS             complement(204..1166)
                     /locus_tag="U2R41_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8890047.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1168..2607)
                     /locus_tag="U2R41_002712"
     CDS             complement(1168..2607)
                     /locus_tag="U2R41_002712"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEM8890048.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2792..4738
                     /locus_tag="U2R41_002713"
     CDS             2792..4738
                     /locus_tag="U2R41_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEM8890049.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5002..>5117
                     /locus_tag="U2R41_002714"
     CDS             5002..>5117
                     /locus_tag="U2R41_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8890050.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          854 c          840 g         1758 t
ORIGIN      
        1 ccaatttcgt aaaacaaccg taacagttgt gtaaaaatat cattttagag catgaaaaaa
       61 ggagtggatt tctccactcc aaaaaagtct aacttttggg ggtcactaca agttgacaac
      121 tcctttttgt ttacaaatca attggatatc tacaaattgt attttctaac gaattttata
      181 gacattaaaa aattaattta acgttaaata cttcctccag aaagtattga gattggtaaa
      241 atataatcct tatttgtctt ttcgccttta atttttttca gaagaatatc aacaataaga
      301 cttgcaattt catcaattgg ttgtcttatt gttgtcaatt gtgggaaaaa tttctcaata
      361 aaagaggtac catcgtatcc aataattttt aaatcttctg ggatattaag tttaagttga
      421 tgagcaattt tcatagtgag aatggctgtt aaatcatcag aaacaaaaat accatctggt
      481 ttgtgattta taatagttga ctttatttcc atttcacgac ggatagtaga cagattatta
      541 ggaattttga aaatttttcc atttggattt tgaaaagaaa aacctagtgc acgtaattct
      601 gtgggagaat ccgtgttatc atttcctgta atcataatgg tattttgaca accatttttt
      661 tgtaaagtct tagctgcaag ttttccccct tcaaagttat ctgaagaaac aatggaaaca
      721 ttaggagcta aatttctatc aaaagcaaca ataggtgctc tcacacgctc ataatcagca
      781 attccaagat tatggcttga tgagattatg ccatcaactt ggttggcttc aagcatttca
      841 atgtattctc tctctttagc aggatcattt tgactattgc aaataatcgt tttatatcca
      901 tgtttaaata actctatttc gagatactca atgagttcag agtaaaagat attactaata
      961 ttaggaaaaa taagaccaat aagctgaggt gactttcctt gaagtccacg tgctaaatta
     1021 tttggtttat aaccaagagt tttcatagct tcatgaactt tttgttttgt tttttctgat
     1081 aaatatccct tattattaat gacgcgtgaa acggttgttg ggctaactcc agctaattca
     1141 gcgacatcag ttaattttgc taccatttta atattttaat tcaaagtatt ttccagtagg
     1201 ttcgcctgat ttgatttgaa taccattttg gtgattttca gggaatacac gactagtaaa
     1261 cactttttct cctttattaa taaaaatctc aatgattgag ttatctacaa aaatgttagc
     1321 agttgtttct ttagtatcaa ttgagcattc acgaatcgta ccaaagtcag tggcatattg
     1381 aatgcctgct ttgctgcgat ctaaaataat ttttccttct ttggtatcta caatcaagct
     1441 aagtccattt cctttactat cagagaaaag aagtaactcc gatttctgat ttggaggaaa
     1501 agttagttca agctcataag tattatttgt ttctgctttt gctgtaaatt gttcagaact
     1561 agcacgtaat gatgtaatag cttctactgg atattggtaa agtttaccat cttttattga
     1621 aagttctttt acaagactca ttgctccctg ataatcatac ttatcagttg gataatcgat
     1681 atcaggtagt ccaatccaac tgacaatcag agcacgacca tcaggagtat tgaatccttg
     1741 agtcgcatac gcttcaaatc cataatcaag attttgaatt tctgaagcgc ccacaagttt
     1801 tactttttct atgtcaagcg attgacagat cttaaaagta ttcggataga tattcccata
     1861 atttagctca gatttatcca acccttgagg acaataaatg agaacaggtt ttttgtctac
     1921 aaatactaga tttggacact caatcatgta ttctgaacca gtgccgccaa aatcaaggtt
     1981 gccaacttcc tcccatgttt ccacgttatt gtcatccgct ttgtaaagtt tgataaagcc
     2041 tgatttttct aaattttgtg ctccaacaat cgcatacaat tgtccttcat aattaaacat
     2101 ttgtggatca cgaaaatgat cagttacatc agcaggttta tgaataagaa cattctcacc
     2161 tttcgtaatg ttgtagtctt tatccatcca tgctccgatt tgaagtgtat cacgttccca
     2221 attttcattt cgaacgtttc ctgtatagag taagaataga ttcccctcta tttcatatgc
     2281 agaaccggaa taagcgccgt ggctatctcg ttttgtgtct ggaagaagtt ctgctcctgt
     2341 tacatgaaaa tgaactaaat catctgattc attgtgtacc cattctttca acccatgagc
     2401 agctccaaat ggccaatttt gataaaaaag cgtatatttt ccgttaaaaa aagaaaaacc
     2461 attaggatcg tttaacaatc ctgttttagg ttcgatatga tagctagcac gccaaggtga
     2521 tcgttttaca ttatccttaa ttttatccat ctcatcttta gtccaatcag aataagcacg
     2581 atatcgtatt tctcgaggta agttcatatt ttactccttc aatttatctt tttacaacta
     2641 gtatggtaaa cgttttccgt tgaaaaatca atcatttcat ttaaaaaaat aaactatatg
     2701 acaaacgttt gacatatttt taagatatgc taaaataaat ttgcatttca gtgaaccgtt
     2761 ttcacgtaaa aataaaaaag gagtgattcg aatgaattac agtaaagtag cttccgacgt
     2821 aatacaagcg gtcggaaaag ataaccttat tgcagctgca cactgcgcta cacgtcttcg
     2881 tctcgtacta aaagatgaca cgaaagtgaa tcaaaaagct ttagacgaaa atccagatgt
     2941 gaaaagcaca tttaaaatta atggacaata tcaagtgatt atcggtgcag gtgatgttaa
     3001 tttcgtttat gacgaactta tcaaaaaaac tggactttcg gaaatttcaa cagatgattt
     3061 aaaacaaatt gctagtaaag ataaaaaatt caaccccatt atggcattaa ttaaattatt
     3121 atcagatatt tttgttccaa ttatacctgc ccttgttgca ggtggtttat taatggcatt
     3181 acaaaacttt ttaacagctg ctggactttt tggaccaaaa tcaattgaag aaatgtatcc
     3241 tgcgattgaa ggtatttcct caatgatcca actgatgtcc gctgcaccat tcatgttctt
     3301 accaatttta gtaggtattt cagctgctaa acgttttgga gcaaaccaat ttcttggtgc
     3361 tgcaattggt atgatcatga caacaccaga tcttggtggc tcaactgaat attggaatat
     3421 ttttggttat catgtttcac aaacaaacta tgcttatcaa gttattccag ttttagcagc
     3481 tgtttggata ttatctatat tagaaaaatt ctttcataaa aaattgccat catctattga
     3541 ctttacattc acgccattat tatcagtaat gattactgga tttttgactt tcacagttat
     3601 tggccctgta atgttattgt tgtctaatgg aattacagac attattgttt ggctatataa
     3661 cactactgga tttattggaa tgggtatttt tggaggaaca tattcactta tagtcatgac
     3721 tggtttacat caatcatttc cagctattga aactcaatta ctttcagcgt ggacaaatgg
     3781 tatcggacat ggagacttta tctttgtagt tgcatctatg gcaaacgttg ctcaaggtgc
     3841 tgcaacattt gccatttggt tcttaactaa aaattctaag acaaaaagtc ttgcttcctc
     3901 tgcgggtgtc tcagcacttc ttggaattac agagcctgcc ttgttcggag ttaacttaaa
     3961 ataccgcttt ccattctttt gtgctttaat tggttcagga attgctacag cagtagcagg
     4021 attacttaaa gtagttgctg tttcacttgg ttcagcaggt ttcttgggat ttctttcaat
     4081 taatgcaaca tctattccat tttacgtatt gtgtgaattg attagctttg tagttgcttt
     4141 tgcaattact tacttctacg gtaaaacaaa agctgctggt atctttaaag cagaagaaat
     4201 tgctgaacaa acaccagtta attcttcaaa agtagcaaca acagatccaa tatctactga
     4261 tgttgaatca acacaaacaa aagtttcatc agaaacaatt gttagtccca tttctggtaa
     4321 agtgattagt ctttcttcag taaatgatcc agtcttttca tcagaagcta tgggaaaagg
     4381 gattgccatt aaaccaaatg gaaacacggt ccattctcca gtagatggta ctattcaact
     4441 cacgtttgaa actggacatg cttatggtat taaatcaaat aatggtgctg aagttctaat
     4501 tcatattggt attgatactg tatcaatggg aggaaaagga tttacacaaa aagttactac
     4561 taatcaaaaa gttagaaaag gcgatattct tggagcattt gaccgttcaa tgattgagaa
     4621 agaaggtctt gatgatacaa caatgattat tgtgaccaat acttcggatt attcagaagt
     4681 aaatatagta gctgaaaata cagtcactga gggtactgat ttacttgaaa tcaaataaat
     4741 atatttccct gagtaaagat ggaattttct ttccaaaatc aaataatata gattaatcaa
     4801 atatttgaga ataacatttt atttaatctt ttcatatatc aattgtatga aaaattatta
     4861 gaattttaaa aaatgataaa aattcaaaaa gaaaaaatag tagttattta cttgtcatgt
     4921 tactaaataa aaaaataatt taaatatgat atatagtcat ttatttagtg atttcattct
     4981 aaaataaagg gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg cacaaaattt
     5041 gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt tccgacaacg
     5101 acacctgagg aaacaat
//
LOCUS       DASHWH010000121         4807 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.128, whole genome shotgun sequence.
ACCESSION   DASHWH010000121 DASHWH010000000
VERSION     DASHWH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.128"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U2R41_002715"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U2R41_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEM8890051.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="U2R41_002716"
     CDS             complement(3250..3768)
                     /locus_tag="U2R41_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEM8890052.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DASHWH010000122         4805 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.129, whole genome shotgun sequence.
ACCESSION   DASHWH010000122 DASHWH010000000
VERSION     DASHWH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.129"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..411
                     /locus_tag="U2R41_002717"
     CDS             <1..411
                     /locus_tag="U2R41_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890053.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="U2R41_002718"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="U2R41_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8890054.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="U2R41_002719"
     CDS             2446..3708
                     /locus_tag="U2R41_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8890055.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4805
                     /locus_tag="U2R41_002720"
     CDS             3953..>4805
                     /locus_tag="U2R41_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8890056.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1658 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaa
//
LOCUS       DASHWH010000123         4802 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.130, whole genome shotgun sequence.
ACCESSION   DASHWH010000123 DASHWH010000000
VERSION     DASHWH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.130"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(49..1197)
                     /gene="nagA"
                     /locus_tag="U2R41_002721"
     CDS             complement(49..1197)
                     /gene="nagA"
                     /locus_tag="U2R41_002721"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEM8890057.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1273..2109)
                     /locus_tag="U2R41_002722"
     CDS             complement(1273..2109)
                     /locus_tag="U2R41_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8890058.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISN
                     NL"
     gene            complement(2293..3510)
                     /locus_tag="U2R41_002723"
     CDS             complement(2293..3510)
                     /locus_tag="U2R41_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8890059.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3659..3838)
                     /locus_tag="U2R41_002724"
     CDS             complement(3659..3838)
                     /locus_tag="U2R41_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890060.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3893..3976
                     /locus_tag="U2R41_002725"
     tRNA            3893..3976
                     /locus_tag="U2R41_002725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3927..3929,aa:Leu,seq:gag)
     gene            complement(4216..4794)
                     /locus_tag="U2R41_002726"
     CDS             complement(4216..4794)
                     /locus_tag="U2R41_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890061.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1459 a          999 c          775 g         1569 t
ORIGIN      
        1 ttaaaaccaa acttaagaag tttagtttta aggttaactt aaattatatt atgcgtggaa
       61 acgttccacg ccatctagat aagttgcttc caatgtcata tcagggttca atacgataaa
      121 gtctgcatcg cgtccttgag cgatttttcc acatgtatca tcgattttac agctgattgc
      181 tggaatcaga ctagccatca tgactgcttg ttctggtgtt gctaatcccc agtctacaac
      241 atttttaatt gcttctttca atttcaatat acttccagct aagttgccag attctagacg
      301 tgctgttcct tcttttacta ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat
      361 cccaccggcc atcatacagt ctgtaattaa tgcgacatga tcatgtcctg ctttttccat
      421 taggattccc gcagcatttg gatgtacatg atggccatca caaatcaatt cagaaaagac
      481 atgttctaaa gataataacg cacccaccat acctggttcg cgatgattca atccacgcat
      541 accattgaaa gcatggacaa atacacttgc gcctgcttca acagcttttt gcgcttcttc
      601 tagtgtagca ttactgtgtc ctagagaaac aactactcct tcttcagtca cttgtttgac
      661 aaactcttct acaccttgac gttccggtgc taaagcaatt tttttgatta aaccacctga
      721 agcttcttgc cattcatgga aagtatccaa atcaggatcc ccgaaataac ttgggttttg
      781 tgcccctttg tgttcttctg tgaagaaagg accttcaaaa tagattcctt tgattttagc
      841 tcctggtgct tcctgataca ttttgccgat tgtctcagct acatctttta aacgttcttt
      901 gcttgacgtc aatgttgttg gtaaaaatga agtcactcca caagataaca gaccttcaga
      961 catcactttg atgccttctg catcattatc cattacatca tgattcatat agccgtgaat
     1021 atgtgtatct accaaaccag gagcaatcca tttgccgctt tggtcaatga tgttgacttc
     1081 tccctcaggt agttctttgg tgtattcacc aaaaagtcca tcggtaattt caagatagcc
     1141 tgcatgtttg acacctgacg ctaaaaagaa tttgtcagca tagataaaag ttctcattct
     1201 attctctccc tttgtttttc tacctctaaa gtactcttat ttagagaaga agtcaagaat
     1261 ggtctacacc atttacaaat tatttgaaat ttgtttcaca gcttgtgcta agccttcata
     1321 gaatgctgct ctttctggtt cttctttacg cttgtatatt aagcttttaa gcacaaataa
     1381 atcattgagg taatagtagc tattcttctc ttgagcccaa tagattcctt gattcacctg
     1441 ttctaacgct tcgtcgattt cattttgatg gaataagaaa tttccataat tataaaaaat
     1501 ttgcgaaaat tcactttttg atcgttcatc agctgattga tgcagcagtt ggatgcttcg
     1561 actcaaatag tagcgtgctt cagccgtttt tcctgccaca ccatatattt ttccgataca
     1621 actaatcagt tgtatttctg tgtcagaaac atggattctt tctttttggc tagagtagct
     1681 taatgcttct ttcaaataat aaagggactg agagatattt tgacgcaacg tgaattcgca
     1741 aacaccgaga taataataat agcgttgaaa gtcagttgct tgatacagat tttcagaaat
     1801 atcagaagat tttagtactt gttctaactt ccgataatca cgtttttgga aacaactatc
     1861 caacagttca aataatttat cgtttgtccg agtagttttt cgagagagat tcatcacatg
     1921 atcaatcgtt acccctagcc gttgacaaat ctgcatcatc accaatacat tcggcagttc
     1981 ctcattattt tcgatccgac ttaatacact ttgtgaacaa atatcatgcg aaagcatttt
     2041 ttgcgtcatt ttttgttctt ttcgaatctc tttaatgatc gttccaaagg actcgattaa
     2101 cgtttccata agtatcctcc ccaaaatatg cgtattcgca ttctattcgt attttacttt
     2161 aggaagtcgc aaacttcaag aaaagacttg caactaaata atatacttta gttatttaag
     2221 aaaactccac tagaaagaga gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt
     2281 tggtttttaa aatcattttt ggcgataggt tgctaggaat tctctcattt tcttagctgt
     2341 ttgtttcaaa tcatccattt taggaagata aacgattcga aaatgatctg gctggttcca
     2401 attaaatcca ccaccatgta caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa
     2461 tttttcatca tccaaaatat tgaatcgctt tgtatcaatt tttgggaata tataaaaagc
     2521 agcttttggt tttacggctg aaagaccagg aatatcattg attgcattat agatatattc
     2581 tctttgttca tagatccttc cgcctggcag gagcagctca tcgacacttt gataaccgcc
     2641 tagcgcggtt tggataattt gctgagacaa tacattggaa cacaaacgca tggaggacaa
     2701 catattcagc ccttctatat agcctttgac gtgtgactta tctccactta ggaccatcca
     2761 gcctactcga aatcctgcta cacgatgaga tttggataga ccgttcagcg taacaacgaa
     2821 caaatcaggc gctaatgtag caatcggaat atgttctaga ccatccatca ctaagcgatc
     2881 atagatttcg tcggaataaa taatcaggtt attttgtcga gctacttcaa cgatttcttc
     2941 taataattct tttggataaa gtgccccagt aggattatta ggattaatga taacaatagc
     3001 ttttgtccga ctcgttattt ttgatttgat atcatcaata tcaggatacc attctgcctc
     3061 ttcatcgcaa atataatgaa caggtgtgcc tccagctaat gcaatagaag ccgtccacaa
     3121 cggatagtcc ggcattggaa ccagtacttc atctccattg tccagcaacc cctgcataca
     3181 catcgtgatc aattcgctca ctccgttgcc ggtgtagata tcattgatcg tcacattcgg
     3241 aaaatgtttc agttggcaat attgctcaat tgcttttcta gcagaaaaga tccccttgga
     3301 atctgagtag ccttctgagt cacgggcatt catgatcaaa tcacgaatga cttcgtttgg
     3361 tgcttcaaat ccaaaaggtg cgggatttcc cgtgttcaat tttaaaatac taatgccctc
     3421 ttcttgcatc cgctccgctt cttctagaac gggaccgcga acatcgtaac tgacaccttc
     3481 taatttgttg gatttttcaa attctctcat cacttagcca atccctcatt tcaaaaagtt
     3541 agaactttcc aaattttttg ttatttacta tttcaatatc atactccatc ctagaaaatt
     3601 ttgccactta tttaggaaaa acgtaagtat taaaaaaaca caaacaagcg ttgagaactt
     3661 aattgtcatg atagtcattc catagattcg ctgcccaatc cattgcaatt ccacaaatga
     3721 ttcctagcat aaaaccaagc aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga
     3781 tcattcccag tcctgaaaat tctccgtcat atttctccat cttaataagt cctcccattt
     3841 ttgagcttga caactatgta aaacctcgct atcattagtg tgttattatt acgcagatgt
     3901 gttggaattg gcagacaagc atgattgagg gtcatgtgag cgttagctcg tgtgggttcg
     3961 agtcccatca tctgcactac aactcgtgaa gttaattttg cttcacgagt ttttttgttt
     4021 gaacgtttct ttatgccatt atatacatat cactgtatcc tttacaaaaa gaacgtacaa
     4081 tcatgtaaac agcctgaggt gagtatatga aggtcggaaa caagctactc gtagaaactt
     4141 gcaagtgtta aaacaattaa tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga
     4201 gggatttttt gtatattatc tgtttatcac cgttattata aaggtacaaa taccaagcac
     4261 ggttaatcca aattgcataa attcattttt ttgtacactc atgcttttca ttagctcttt
     4321 aactgccatt agtactaaga tggcgataac aacataaagt atatagtacg tccgcattcc
     4381 ggaagcccat actgtgggag aaaatcccat aattaacctt gaacaaaatc ccattatcat
     4441 caaaataact aaaaaaatag aagttttctt attgtcaaaa cttaaataaa ttccaacaat
     4501 taaacatagt aataatgcca agactactaa agtagctatc caagtacctg gataaaaaag
     4561 tgacagtttc gttcctaact ctgtaaagag attattcagg ttactactac tccaaatcat
     4621 tgccctttta tttccattga cgtaagtaaa gctttgaccc attgtattac caaaataaat
     4681 aatcaggttt aagaaaaacg gtaatgcagt caatatacgc acataatagt tttgacattt
     4741 cctatatgtt agaaggaaaa ttaagaaaaa tagcaataaa aataagatat tcatgtctaa
     4801 aa
//
LOCUS       DASHWH010000124         4744 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.132, whole genome shotgun sequence.
ACCESSION   DASHWH010000124 DASHWH010000000
VERSION     DASHWH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.132"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            25..696
                     /locus_tag="U2R41_002727"
     CDS             25..696
                     /locus_tag="U2R41_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8890062.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="U2R41_002728"
     CDS             755..1195
                     /locus_tag="U2R41_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890063.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="U2R41_002729"
     CDS             1207..1875
                     /locus_tag="U2R41_002729"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM8890064.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="U2R41_002730"
     CDS             1939..2562
                     /locus_tag="U2R41_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEM8890065.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="U2R41_002731"
     CDS             2618..3004
                     /locus_tag="U2R41_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEM8890066.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="U2R41_002732"
     CDS             3217..4608
                     /locus_tag="U2R41_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEM8890067.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DASHWH010000125         4705 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.133, whole genome shotgun sequence.
ACCESSION   DASHWH010000125 DASHWH010000000
VERSION     DASHWH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.133"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            544..1107
                     /locus_tag="U2R41_002733"
     CDS             544..1107
                     /locus_tag="U2R41_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8890068.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="U2R41_002734"
     CDS             complement(1386..1673)
                     /locus_tag="U2R41_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8890069.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="U2R41_002735"
     CDS             complement(1663..1992)
                     /locus_tag="U2R41_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8890070.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="U2R41_002736"
     CDS             2297..2626
                     /locus_tag="U2R41_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8890071.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="U2R41_002737"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="U2R41_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEM8890072.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="U2R41_002738"
     CDS             complement(3551..4462)
                     /locus_tag="U2R41_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM8890073.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1546 a          801 c          707 g         1651 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctcta
//
LOCUS       DASHWH010000126         4617 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.134, whole genome shotgun sequence.
ACCESSION   DASHWH010000126 DASHWH010000000
VERSION     DASHWH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.134"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..964)
                     /locus_tag="U2R41_002739"
     CDS             complement(17..964)
                     /locus_tag="U2R41_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEM8890074.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1142..1945)
                     /locus_tag="U2R41_002740"
     CDS             complement(1142..1945)
                     /locus_tag="U2R41_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEM8890075.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2091..3005
                     /locus_tag="U2R41_002741"
     CDS             2091..3005
                     /locus_tag="U2R41_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM8890076.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3053..3523
                     /locus_tag="U2R41_002742"
     CDS             3053..3523
                     /locus_tag="U2R41_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEM8890077.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3608..4216
                     /locus_tag="U2R41_002743"
     CDS             3608..4216
                     /locus_tag="U2R41_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEM8890078.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4350..4550
                     /locus_tag="U2R41_002744"
     CDS             4350..4550
                     /locus_tag="U2R41_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8890079.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1512 a          716 c          911 g         1478 t
ORIGIN      
        1 atattgtttt cagctattaa tatcctgcag acaattttac ttgattggtt tgaagctcat
       61 gggtggcaaa gtagcttttc agattggtta aaaaaatagc cattaatttc ttttgaaaat
      121 caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt gtccattttc caaagaggat
      181 gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc tgcaaaggaa agttgctggt
      241 tatttaaagc ctgcaccaga tcttgtgtct taacagaagc accacggcca acattgatga
      301 atacagattt tggatcaaag tgttcaaaca tatgctgatc aaagagccca gttgtttcct
      361 ccgttagagg taagatattg actacgaaat ctacttcagg aagaacttcc tttagttgat
      421 ttaaagaaaa agtcttttcc attccttctg ctggatgacc ggatgtattg attccatagc
      481 agttcatgtc taatgctttg catttttttg caatcatttt tccaatatgg ccagttccaa
      541 tgattagtat ttttttccct tggatagatt ggtaatggac cgatgaacct aaccatttca
      601 tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga atacccaagg atgattccta
      661 agacatgctc cgtaatcgat tcactgtgga tgccactacc atttgaaagt aaaatatgtt
      721 tctttgaaaa agtctcaagg ggtagctgat caacaccagc tgagatagat tggacccatt
      781 tcaaggtaga agaggctaat agtttttctt ggtagtctga gttccagccc aaactgatct
      841 ctacattttg tagatcctct ggttgtaatt ctgttttaaa gatataacca ggcgctatag
      901 tatggatttc ttcgataaat tcatcacgaa atggacgatc tgcataaatg attctttctg
      961 acatagttaa tttcccgcct ttctttactc taagtgtagc gcatacaaga agaactttga
     1021 agcgccttgc ttgttttttg agaaagaaat atttttcaaa taaaaaaggg gctgtgacaa
     1081 aaaaatgtca caacctctaa aagggtctat tgaagtagaa ataacgagtt gtctttattt
     1141 cttaattgat cgatattttt gcccatcctt tgacagcttg agcaaaaata aagattcctg
     1201 caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa gaccatcaag attgttgcaa
     1261 ttatagtagg caatgaaatt gaatagatca atgtttttaa acaatcaaga aaagtagctt
     1321 ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt ggtaaatagc aaagtgatca
     1381 gcagattcag aaaactagga taaatagaaa atataaatgt caggactttt atccaaagtg
     1441 gtatagatgc ttcacttaaa aaagaacgga gctgcttacc tgttaagttt tttaaagatt
     1501 cattggtgta gggaagttct aattggttat tgttcaacaa agcagaagtc gtgcctgtat
     1561 taggaagagc tatgataagt ttgtttttta acatcccgac actaacaaaa ttccccatca
     1621 aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg atcaaatgtg aagataatgg
     1681 agtttgtttg gtaaatgaat ccttcttttt cttcggtttg aagctgcccg ttttggattg
     1741 taaaatcagg aatgcgagta gcaatctttt gcccatccaa ttgaatgttg tttaagactt
     1801 gaaaaacttg aaaagagatt ggcaaagcca taattatact caaaactaaa agatagacaa
     1861 tggctttcca aaatgggaca tttctcgcct cctttaggtc tttgaatcgt ataaaggaac
     1921 tgataattaa ttgttttgtt gtcataagta ctcctcatct agtagtgtca tcacttgcaa
     1981 ttctagcgaa gtatcaagtg aaatacaagt gctttaagga aacagctgaa tgattggtga
     2041 aatatcaaaa aaatgttatt cttgtctaaa gactatccaa tataaataaa atggaggaag
     2101 gaatagtgcc tacacagcca cagagatcat caaaaaaact atttggagta gtggttttga
     2161 atgcggtgat cactttatca gaatttattg gagggattat gtcagggagt ttgtctcttg
     2221 tttcagatgc tttccataac cttagtgata cattggcaat tattttttct tacggggcac
     2281 aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta tggctaccag cgtttagaaa
     2341 tattgagtgc tttcatcaac tcattcatat tgatcatatt atccttgttt ttaaccgttg
     2401 aagcattcaa gcggttcact tcaccagaaa agataaatag tcatttgatg ctaacagtag
     2461 cagttattgg attgctcgcg aatttgttct cagtattatt attacgacaa gaagcagatg
     2521 aaagtttgaa tataaaatct agttatttgc atttgttaag tgatacactg tcttcggtaa
     2581 gtgtgattat cggagcaatt ttgatccgtt ttttcgatat ctactggatc gatccagtta
     2641 ttacactgat catttcgata tatatattga tagaagcgat aatggtgatc aaaaaagctg
     2701 ccgctatttt gatacaaagt gcacctgcta tcgactatga aaaaatggaa caagaaattg
     2761 aagcaatcga tggggtaaag gatgttcatc atgtccatat ttggcagtat agcgaaaaga
     2821 taattatttt cgacggacat attgattttg aagatcagct actaagcgag attgaaaaga
     2881 tatatcctcg tataatttct ttattaaaat taaaatatgg tattacacat gtgacgatcc
     2941 aagcagagac aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa gatgaaagtt
     3001 attgaaatat agagtatgat aaataagaat ttttaataag gtaggttggc tcatgaagca
     3061 gtatcgacag ttaaaaggat atttagaagc aaaaggatat tgggagatac tagtctatct
     3121 cttttttggt ggtttggcta cgattgtaaa ttttgttagt tttgctctcg cacgccaata
     3181 ttttgattta agtatggcct tatccaattc tatttcgtgg ttttgttctg tattgtttgc
     3241 ttttgtaacg aacaaacttt gggtattcca ttcaaaatca ccgaatttta cacatgcact
     3301 tattgaatgc ggtaagttct tcttctaccg tatcctttct tacggattgg acatgggggc
     3361 aatggtattg ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa tcatcactca
     3421 aattattgtt atccttgcga actatatttt cagcaagttg tttattttta aagaaactga
     3481 ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac taggcataac aattgaagaa
     3541 tttccttctc tttgttactc tttgtaatgt agacattatt aagaaaacat tgaggaggaa
     3601 tttttttatg acttacacat taccagatct accttatgca tatgacgcat tggaacctta
     3661 tattgacgaa gaaacgatgc atctgcatca tgataagcat cacaatactt atgtgacgaa
     3721 tttaaattca gcaattgaga aatacccaga attaggcgaa aaaacaatag aagaattatt
     3781 atctgatatg gacgctattc caacagatat caagacagct gtacgtaaca atggtggcgg
     3841 acatgctaac cattcatttt tctgggaaat catggcacca aatgctggtg gcgaacctac
     3901 aggagaaata aaagaagcga ttaatgaagc ttttggtgat ttttcttctt ttaaagaaga
     3961 attcaaaaaa gcagccgctg gacgatttgg ttctggatgg gcttggcttg taatggaaaa
     4021 tggaaaatta gctattacct ctactgcaaa tcaagattct ccattgatgg aaggaaagac
     4081 accaattcta ggtttggacg tttgggaaca tgcttattat ttgaaatata aaaatgtccg
     4141 accagattat attgcagcat tctggaatgt tattaattgg gatgaagtaa acaaacgttt
     4201 cgaagcagca aaataaccat ttcctccgtc atatcagtct taaaaataat ttggaaaata
     4261 aaagagcgaa agcttgctag cgttttctaa attgggtata atgttcttgc aaggttactt
     4321 gcaagaactt ttttggagga tatacattta tggaacacgg aacggttaaa tggttcaaca
     4381 atgaaaaagg atttgggttt attacagtag acggcggaga tgacgtattt gttcatttct
     4441 cagcaattca aggagacggc ttcaagagcc tagaagaagg tcaagaagta gaattcacga
     4501 ttgttgaagg tgcacgcgga cctcaagctg ctgaagtgac taaattataa taatttgttg
     4561 aataaagaca cgacttctta gagagtcgtg tctttttgtc tatttatgaa gttttta
//
LOCUS       DASHWH010000127         4503 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.135, whole genome shotgun sequence.
ACCESSION   DASHWH010000127 DASHWH010000000
VERSION     DASHWH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.135"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            526..2337
                     /locus_tag="U2R41_002745"
     CDS             526..2337
                     /locus_tag="U2R41_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890080.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2398..3858
                     /locus_tag="U2R41_002746"
     CDS             2398..3858
                     /locus_tag="U2R41_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM8890081.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3869..>4503
                     /locus_tag="U2R41_002747"
     CDS             3869..>4503
                     /locus_tag="U2R41_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8890082.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQ"
BASE COUNT         1717 a          736 c          974 g         1076 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgag
       61 aagatgtttg aacaccagag aaagaccaac ggtcagccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agaacgttta gaaaacaaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggctataaca agtacggcgg cctggatatg acaatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cagaaaaagg aaaagaaaat
      421 cttcttcctg aagtacaagc aattattgac agcggacgag tgacgcaatg gaaaaaaaat
      481 ccaaccattt attttttgaa agactatcga aaagttgctt tagaactgaa tgaaaacggg
      541 ggatttgagg aatcaaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
      601 gaattacttg aagaggctgc gccaactgat tctttgaagg agaaagaaaa cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tagaattgat cgatcaactc aaaacgttgg ataaccaata cgccgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcataccc attctcctga agacgtgaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ctgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaacct
     1441 gaagactatc cgaaaatctt tccgaaccgt gtctttgatt ttcaattgga taaaaaagga
     1501 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattggaat acaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcta caagtattca tgaattagcc catgcaagaa tgcacaaatt ttcagaatta
     1741 gataccgcaa ctaaagaatt gcaggcagaa atgacttctt atattgtgtg caaacatttt
     1801 ggaatggaca cttcggagaa agctattcct tatattgcag catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagagaat
     2041 aagaatattg tgcccgttgg ttcactttgg atcgacaaga aagacgggaa agtaatggaa
     2101 gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt ttattgaaga tccgcagcat
     2161 aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga ttcaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa tccattccga ttttcacttg aaacagtgtc agaagtggcc
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg cgtataaaac
     2341 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg tttgaaaatg
     2401 accgaaaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
     2461 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
     2521 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
     2581 gacattattg aattagctaa actttttttc atagacaaag agatccaaga ctctaagcaa
     2641 caatttaaag cagccacaga ttttattcta aagaatgaag acaaaacaga acgagtagaa
     2701 aatcttcatt ttgagacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
     2761 gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac cagagtggct gattgattat
     2821 gcagaaaaag aagggttgat tgccgaacta aagccaaaac atgaacggca aaatttctta
     2881 gttcgagacg atcgtttaga tcatgcggtc gcctttttat ggaaagatcc acagacaaat
     2941 gagacagtag gggcgagtta tcaaggaacg ttcattgatt atgaacgttt tggtgagaga
     3001 ggcacatata agcatataga taaaaattcg acagcgaatc atggctttaa cctaaagatc
     3061 ggtgacccta agcagttaaa attttttgag agtagtattg atttacttag ctatgctgca
     3121 ctaaatcgtg atcagttaaa cgatacgtgg ttagtttcga tggaagggtt aaaacaccat
     3181 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
     3241 caatccatcg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
     3301 caattaatgg gcgcagttga tccatttacg aatcagaaag tacgttgtga acgaggaatc
     3361 gctaatgatt ggcaagtgcc aaaagaatac aaagtcattt atgaagaagt cgcaaaagaa
     3421 gaaaagatca caccagaagc tattatggcc atacacaaga cagaaaacaa tctgcaatta
     3481 acgaaccaat tggtttccgc tcacaaagta aatgcgtctt ttggtcagca gctttctgtt
     3541 aacgattcaa tagaagctat taatttgaaa gatatttgta gagaagtcgc aaaagaatta
     3601 aaagcttgtg aacgcgtgga tggtacgtac gattttgatc gtttttatca agaaaaaggg
     3661 gatattaatg ctcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
     3721 aatcatgaac atgaatttgt acctgaagta aaaaaggatt ggaatgatca attgaagcat
     3781 gaaatctacc aacaagagat cagaaagcaa aaacgtgcca tgttgtttca acaaggtaga
     3841 cagcaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt attttagcag aaaaaccgtc
     3901 acaagcgaag gcttatgccg atagcttttc taaagctacc cgaaaagatg ggtattttga
     3961 gatccaagat cgattgtttt caggagaaac tgtgatcacg tatggttttg gtcatttagt
     4021 cgagttagat tcgcccgata tgtacgatga aaattggaaa caatggtcat tagagcattt
     4081 gcctattttc cctacacatt atcactatca tgttcctaaa gataagaaaa agcaattcaa
     4141 ggtggtgaaa cagcaacttc aatcggccga caccattatt attgcgacag atagcgatcg
     4201 tgaaggggaa ctgatagctt ggacgatcat tcaacaagca ggcgccgatc atggaaagat
     4261 atttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagg gattccagca
     4321 gttacgagat gcagaagaga cctacccaaa atttgaagaa gcacaagcac gccagatcgc
     4381 cgattggttg atcggcatga acggtagccc gctgtatagt ttactcttgc agcaaaaagg
     4441 cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca tgatttatca
     4501 att
//
LOCUS       DASHWH010000128         4461 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.136, whole genome shotgun sequence.
ACCESSION   DASHWH010000128 DASHWH010000000
VERSION     DASHWH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.136"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            574..1008
                     /locus_tag="U2R41_002748"
     CDS             574..1008
                     /locus_tag="U2R41_002748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890083.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="U2R41_002749"
     CDS             2298..3221
                     /locus_tag="U2R41_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM8890084.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="U2R41_002750"
     CDS             3237..3776
                     /locus_tag="U2R41_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM8890085.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DASHWH010000129         4446 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.137, whole genome shotgun sequence.
ACCESSION   DASHWH010000129 DASHWH010000000
VERSION     DASHWH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.137"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U2R41_002751"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U2R41_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEM8890086.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="U2R41_002752"
     CDS             complement(2944..3378)
                     /locus_tag="U2R41_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890087.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4446)
                     /locus_tag="U2R41_002753"
     CDS             complement(3395..>4446)
                     /locus_tag="U2R41_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEM8890088.1"
                     /translation="IKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFK
                     LKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYT
                     ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIP
                     SVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKV
                     RDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRES
                     FKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSP
                     KQYQLGLVDNFNNIS"
BASE COUNT         1376 a          825 c          612 g         1633 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatat
//
LOCUS       DASHWH010000130         4433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.138, whole genome shotgun sequence.
ACCESSION   DASHWH010000130 DASHWH010000000
VERSION     DASHWH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.138"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(543..2048)
                     /locus_tag="U2R41_002754"
     CDS             complement(543..2048)
                     /locus_tag="U2R41_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8890089.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEYEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U2R41_002755"
     CDS             complement(2045..2731)
                     /locus_tag="U2R41_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8890090.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2R41_002756"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2R41_002756"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEM8890091.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcatattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DASHWH010000131         4424 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.139, whole genome shotgun sequence.
ACCESSION   DASHWH010000131 DASHWH010000000
VERSION     DASHWH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.139"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1127)
                     /locus_tag="U2R41_002757"
     CDS             complement(<1..1127)
                     /locus_tag="U2R41_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8890092.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1156..1542)
                     /locus_tag="U2R41_002758"
     CDS             complement(1156..1542)
                     /locus_tag="U2R41_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8890093.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(1558..1872)
                     /locus_tag="U2R41_002759"
     CDS             complement(1558..1872)
                     /locus_tag="U2R41_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8890094.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="U2R41_002760"
     CDS             complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="U2R41_002760"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEM8890095.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3132..4421
                     /locus_tag="U2R41_002761"
     CDS             3132..4421
                     /locus_tag="U2R41_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEM8890096.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1313 a          865 c          832 g         1414 t
ORIGIN      
        1 tgatcacgga ttccgtcgcc taaatatttt gcgtctggac gttgacaagc cagtattaga
       61 aagaagccag cttgacgacc taacatgaca atctgtttca gcttattcat aactgcggtg
      121 ttttcttttg ttcccagcat ttccatgaaa gcgacgtatt catcaaagat taagaagtgt
      181 gccgggagac ctaagtaagc ataatttttg ccagtcttat agttcttcat ctgcttcatt
      241 tcctcactac gtttcatcat ttcttcatag aatgtttcaa tgcaagaaag caagtcttct
      301 tttctatagt agacatttgc catcacagaa cctaagtccg caaggtcagc atttttcggg
      361 tcaagaatat acagttttga atctgtatga agcaaggctt caatcagtgt cagtataaag
      421 taagttttac cgccacctgt accaccagca atcaacatat gagggagctt atcatattcc
      481 caccatacgt ttttcattaa gcgaagttta ccatctttag cttctacttc atcaatagaa
      541 atacgactgg ctatggtgtc atagagcaaa gtatattcca cataggaatc ctttaactct
      601 ttatccgtca gctcacagta caagccactc tctaatttct tttccaagtg taagagttgg
      661 tcttgatatt ttcccagcgt gatttccacc cgtatctgta tcaagccatt tttaagtcga
      721 taatacattt tagggaagta ggttatcttt tcctttgtac gaccagcact atctttaaag
      781 aaaccctctg ttttgacctg ttcagattca taccacttgt tttcaagtat catctttgcc
      841 agtttttgac ggtggtaaag ttgtttaacc gtatcatagc gaacccgttt gaatacaaac
      901 gctaccagca agcagataag aattgcgaca ctgaaactga taattaaata gggaatgtca
      961 atcttatctg cttgtgatag gttaaaatcc tgccagttga tctgctggat tgtcttcaca
     1021 tgaaacagtc cgacaaccag caggaaaaca ggcaggagtg acgctatcgt aaaatgaaag
     1081 actaaatctt taccagatgg gcgaatcctt ttaccacgct gtttcatgcg aaaaagtctc
     1141 ctttctacct agcgactatt tgtcttgtgt cggttctttc tttgcttgtg gttgagcttt
     1201 gaatgaacta gaatcctttg tcagcacaat atcgtctgcc ttgatatacc agtcaacatc
     1261 tgctccttga taggtggcag tagcaacggt gtccgcaatg ggattgataa gttccacccg
     1321 tgcgttataa tcaaactctt tcaaaggcac gctggcagga atacttactt gaatcatgcg
     1381 tccttgtcct ttggatttta agtcataggt acgttccttg atttcatctg aaaccgaccc
     1441 gtcttcattt tggattctca cttcacgacg tagagcagag aatttcaatt ctccaaaagt
     1501 cgtgtcttta tctaatacaa tgccatttgc taatctcatc atttttcctc tctttcttta
     1561 ttcttttatc atgtcgtcag catgtaaaag gtaatttgta aaaccacgag tgccgatttt
     1621 gtagccctct gcggtaatac gtggattgac taacttcaca cgttcctcaa agccgaaatg
     1681 tttttcgcca gcttcagcag gaagcaccac cacaatatca tctgctcttt gaacatcaga
     1741 atagagatta tagcttcttg ataagacagt tagccgtccg ttgattcttc gctgaacgac
     1801 tttatcctcg ccagcaaatt ctaaattgcc gaatgttttt tccatgttgg gaatcacaaa
     1861 tttaagttcc atatttttac ctatcctttc ttttttattg gctgaatgaa tgtttgatgg
     1921 tcttaaagag tggggaacga ccttttgatt cttgattttt tgttttcata agttcacttc
     1981 ctttcaaaat cgggtaaaaa aatagacacc tcattttttg aagtgtctac ctattaaata
     2041 ttcaaatttt attggaagta tctttatatc ttcacttttc aaggataaat cgtcgtatca
     2101 aagctcattc ataagtagta aattagtagt aaattgagtg gttttgacct tgataaagtg
     2161 tgataagtcc agtttttatg cggataacta gatttttatg ctatttttct cttaaaaaat
     2221 atcattattt gtggtatgga gcccccgcgt tgatccggaa ggcacggtag atttgctcga
     2281 cgaggatcag gcgcatcaat tgatgtggat acgtcatttt gccaaaagag atctgagcat
     2341 tgcttctttt catgacggct tcgctgagtc ctaaagaacc gccgataaca aaaacaaggt
     2401 ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattcg cttgtgggat
     2461 ttttcccttc aattgctaag ccatagacgt attcttgttc tttgattttc gctaagatcc
     2521 gttctccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc tccggggctt
     2581 tttcgtcagg aacttcgatc aattcgattt tcgcataggc attcagccgt ttgacatact
     2641 cattgatacc ttggatcaag tatttttctt ttaattttcc tactgaaata attttgatat
     2701 tcatgttttc tctcctttga tcttcacgct cactttgttt attataatca attttttcgt
     2761 ctatatgttt attttgacta tttttgttaa aaagatttcc ccaacagtta tgcacaacat
     2821 ccacaggttt atccacagaa aacagcgttg ttttacctta tttcatcaca ttcattaggc
     2881 aaacatacgt tttataagaa tttatccact ttatacacag gctgtggata tgttttatcc
     2941 cttatttttc caatcctatc cgcgtagttt tacacaatat ctgtggaaaa agaaaatcaa
     3001 atattttcat ctgaaaacaa tgatttttcc taaagttttt cataaaaagt tcaaagtctc
     3061 aaagtagact aaagtcaaca aataagacta tactaacagt agataaatga atgtaattgg
     3121 aggaggaaca catggataga aaaaatgtga caccaaagat gaaaaaaaat aaaaataata
     3181 gcctctggcg taagttgggt ctggggcttg ttggagggat cgttggagga ctactgacag
     3241 ctggcatttt ttatgcagcg atgggcactg gcaacaatag ttctactgct acaagcggca
     3301 atcaaaattc tgccggtgaa acagttgtgg aaaatgtcaa agtcaatgta gattctgata
     3361 tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc tgttatcaac ttacaaagtc
     3421 aaaaccaatc aagcggtttt ggcggattat tcgggcagca agaagaaagt agcagctcag
     3481 atgatagtaa tttggaagct tatagtgaag gaagcggcgt aatctacaaa aaagacggga
     3541 atactgctta tgtagtcaca aacaatcacg tagtagatgg acagcaaggt ttggaagtca
     3601 tgatgaaaga cggtacgaag gtaaaagctg aattagttgg aacagatgcc tattcagatt
     3661 tagcggtgtt gaagatcaat tcagacaaag tcgaaacggt tgcctctttt ggggactcaa
     3721 gtgctttgaa ggttggtgaa cctgcgattg cgattggttc cccattagga tctgaatatg
     3781 cgaattctgt cacttcagga atcatttctt ccttgaaccg acaagtaaca agtacgaatg
     3841 aatcgaacga aacagtcaat atcaatgcga tccaaacaga tgcagcgatc aacccgggaa
     3901 attctggtgg tcctttagtg aatatcgaag gacaagttat cggtatcaac tcaagtaaga
     3961 tcgcaagtac ttctgaatcg acttctaatg taagcgtaga aggaatggga tttgccatcc
     4021 caagtaacga tgtagtgaat atcatcaacc aactggaaaa agacggaaaa gtgacacgtc
     4081 cagctctagg tatcacaatg gtcgacttat ctgccgtttc tactcagcaa caagaacaaa
     4141 tcttgaagat tccttcatct gtaactaacg gtgtcatcgt gacaaatgtc caaacagcta
     4201 caccggcaga aaaagctggg ttgaaacaat acgacgtcat tactaagatc gatgacaaag
     4261 aagtttcttc tggtgtagaa cttcaatctg ctttgtataa gaaaaaagtg ggagattctg
     4321 tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac tactgttgaa ttgacgatcg
     4381 atcaatcggc attaaaacaa aatcaaaata gtgaaaatta ataa
//
LOCUS       DASHWH010000132         4398 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.140, whole genome shotgun sequence.
ACCESSION   DASHWH010000132 DASHWH010000000
VERSION     DASHWH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.140"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(96..1616)
                     /locus_tag="U2R41_002762"
     CDS             complement(96..1616)
                     /locus_tag="U2R41_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM8890097.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1859..2044)
                     /locus_tag="U2R41_002763"
     CDS             complement(1859..2044)
                     /locus_tag="U2R41_002763"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HEM8890098.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2028..2210)
                     /locus_tag="U2R41_002764"
     CDS             complement(2028..2210)
                     /locus_tag="U2R41_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890099.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2451..2669
                     /locus_tag="U2R41_002765"
                     /pseudo
     CDS             2451..2669
                     /locus_tag="U2R41_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3164..3763
                     /locus_tag="U2R41_002766"
     CDS             3164..3763
                     /locus_tag="U2R41_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8890100.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3827..>4398
                     /locus_tag="U2R41_002767"
     CDS             3827..>4398
                     /locus_tag="U2R41_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HEM8890101.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1401 a          799 c          785 g         1413 t
ORIGIN      
        1 attcagtagt tttgagcgta agcgtgtcat agaatggtac ctaaacacaa atagcccgat
       61 ttgatacact caaatcaggt tatttgtggc ttatattatt tacagatttc ttgtatctgc
      121 tgattccatg gcaaataagc gcgtagcgct tcaggtgtca tcgaagacag attgggaatc
      181 tctgtaaaaa gatgatttaa atatttgcgg atgtttaacc cattcgcaac agctgtctgg
      241 ataatactca atataattcc atttgatcta gcgcctttta agcttttaga aaatagccag
      301 ttttttcgac cgatcactaa ctctttgatg gcacgctctg ctcggttatt tgaaagttcc
      361 aagcgtccat caagcaacac aatctgaagt ccgtctttat gattgactgc gtattgaacc
      421 gcctttccaa gctgcgattt tggcaaataa gcaaaactct cacaccaatg aaaaaattca
      481 gtcatcacag gctgcaacgt ttccttcctc gctacaaacc tttcttctgc gctaagctta
      541 ttaaaggtcc gttcgattcg aaacatcttg tcacagaagt ctaatcctat tttggcatga
      601 ctttttgggg acggttgttt acccaatgct tcaaaaaact ttctgcgaac atgcgcccaa
      661 caataaacat tgtttaccgg cagctttcca taggccgcgt aaccatcagt atgcaagaaa
      721 ccgctaaatg attctaaaaa actttttggc tcatcaacgg aacgcgaatt tgcgtgctcg
      781 tagagcacaa cttgtttttc ttcgctttta cctgaagcat ataaccagta aaaggtcttt
      841 tctttatgag tattcaatac ccgatagctt gtctcatccg cgtgtaagac ctcctgattg
      901 cttaattctt gatgaaggca attgtagatt ggctctaaaa ccatttcact taccttgata
      961 tgccaattag tgattttttt acgatcaaga gggaaaccaa gtgcatgcca ttctttttct
     1021 tgtcgatatg ccggcacctt tttaacaaat ttttcccaaa tactctgtgt cacaattgaa
     1081 gaggaaccta gactattatt gatcaacggt ttaggaacag gcgctttttt gatttttgtt
     1141 tctcctaatg cttcacattc tctgcaatga taggcatgtt ggtagatgac ttgtttttct
     1201 atatgggcag gaacatacaa tggctcttct ctaagccttg tcgcaccgat atcatgtaaa
     1261 gtatgaccac agtcttcaca caaacaatcg gcacctttta attcataatg aataggaact
     1321 tcaggcagac cttctaatga aagtgctttg agtcccttgc gttttctgcg tttaaaagaa
     1381 gataccgtaa tgggttcctg gctttcttgt ccagtgtgct ctggttcatt aaaagttgag
     1441 tcgtctaata aatcgatttg cccatctacg ggtggtgttt ctttttttcg tccaaacaag
     1501 cgtcgattca tttcagcaat ttgttctcta agaagagtat tttcctgttg ctggatttca
     1561 agcaactgtg ttaattgctt gatttgtaca agaagttcag attccatttt tgtcattttt
     1621 acaccaccat tctagcgatt ttcttacatc tattatacca ttttatgaca acgaaaaaca
     1681 gacaaaatcc ttgatttaac aaggttttcg tctaatatat cgcacctttt ttcgtttctt
     1741 ttagcgtaca tttttcttcg attgaaaggc cagtcagtaa gacttctttt ctggaacgcg
     1801 gccattgaag gtacccactt tcaaatcttt tgtacattaa tacaaatcca ttttgatcct
     1861 agtaaagagc cttgaagcga tctttcttcg taccgcaaaa taagaaaagc gcttgatcgt
     1921 aaacatcaag gtcatattct tctataacga aagctgctag tccatcaatt ccttttcgaa
     1981 gatctgtttt tccacaaacg ataaaaatat gtttcaatgc agttgcatca atgagcattg
     2041 gcattcaact cttttaacaa ggtatacaaa agatatttat ccattccatt ataaataaat
     2101 atttccttat cttccgattt gattctgcaa gctagttttg gcttggtgtc aatttgcgat
     2161 ttttgagccg cacttgaaaa ttgaactggt acaatatctt ttttattcat tcgtttttct
     2221 ccttctatat ttgatagttt aattatagaa gaaagataat cggcgtacga gatactatcc
     2281 tatgacacgc ttacatttaa tttaatattt tgcctcaaat tagccacact ttttatagaa
     2341 ctaagaggaa aaatgaagtt ttcttcctat taatatagtt gtttgaaatc ttaaatatag
     2401 cgttttacga tgtaatggga agtatggtga ataaatcaac tatgttcgta cgaagcattt
     2461 actcaactaa tttaatcgaa tctttcaata aacaaatcaa gaaatacagc caccgcaagg
     2521 aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca
     2581 accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac
     2641 aaatgctgag tcaaccgatg gagaattaga aacaatctac agtagggaag aaccattgac
     2701 acaaaattat tgacgctccc aactaaaagt gtcaaaataa tgatgaaatc atttagttct
     2761 tataaattag aaaagagaaa ataaatattc catatctact ttattggatc cgtaacatga
     2821 ctaaattctg aatacttttt aaagcttatc atttggtaaa actgaatata agttaattat
     2881 atagactgaa aattttgaat tttcagaata gaataactta aaagtactga caaaattttt
     2941 taaaggggca atattaatgt tctgagataa tcaaatccta ccgaaaatat caaaaatcca
     3001 atgtgacaca atcagattgt gtctcattcg agggaatcac acaatcccac gtggctctag
     3061 gcagaattga atttttgctt ggggcttttt ccttatattg attttgaatg agacttatta
     3121 tatgttataa tgcaattaag tatcattgga gagggcgaag aaaatgaaga cggtagaacc
     3181 tattcgagac aaaaaaaaga tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg
     3241 ccaacgaaat ttggttcttt tttcgattgg gatcaacacc gcctatcgca tttctgattt
     3301 gcgtcaattg aagctggccg acgtgctgga aatttcacgc ggtcgcgtgg tggtcaaaga
     3361 tcgattagcg atgaaggaac aaaaaacagc taaacataat tcggtctata tttccaacaa
     3421 attgcgaaaa acgattttgg agtacgttca acaggaattt cctgatcaac tccttcagca
     3481 agattttgac ggttatttgt tccctagtcg aaaagggacg aatgaaccgc tcaaccgcca
     3541 aagtctgtgg cgaatcattc atgatgctgg cacggcaatc ggattgaaag acattggccc
     3601 acattccatg cgaaaaacgt tcggttactt tctctataaa caggggacca aaacagaaat
     3661 cattcagtcc ttgttgaacc attcttctca gcgcgaaaca ttgcggtata ttggtattac
     3721 acaagaagac aaagatacag ctgtaaaaag cttagattta taatataaaa gttaagttta
     3781 aacatggata gttttaaatt agtagattga gtgttgggag gaaagaatga gtacattata
     3841 tattatagga aacggttttg atttaagtca tggtttaaaa actagataca ctgatttttc
     3901 aaaatgggtt gaaaaaagtc atagtgaaat attcgataaa atagacacaa tgataaatga
     3961 aaataatgag gtgagaagta cagctgatga taagataggt tggtttaatt ttgaaagtag
     4021 tttgcatgag ttaattcaaa gtccagtctt tgaagattat gtagaagagt ctaaggatta
     4081 tctcataggt tatggacatg aagattggtc agattctgcc catcatgatt ttcaaaatat
     4141 ggtttatgat tatgttgttt ctctagaaga attacaacaa cttttcaagg aatggatacg
     4201 caaagaaata cagccgacaa tagaacataa aattccaaaa taccaatttt cgtccgatgt
     4261 actattcttt acattcaatt atacagatgt tttggaaatt ctatacaagc aaccgaaaga
     4321 aaacatatat catattcatg gcagttatga cgatataata atcggccact ccgagaattt
     4381 tgacaacttt ataggaga
//
LOCUS       DASHWH010000133         4374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.141, whole genome shotgun sequence.
ACCESSION   DASHWH010000133 DASHWH010000000
VERSION     DASHWH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.141"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(83..1051)
                     /locus_tag="U2R41_002768"
     CDS             complement(83..1051)
                     /locus_tag="U2R41_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8890102.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="U2R41_002769"
     CDS             complement(1498..2904)
                     /locus_tag="U2R41_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8890103.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="U2R41_002770"
     CDS             complement(3126..3488)
                     /locus_tag="U2R41_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890104.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3830)
                     /locus_tag="U2R41_002771"
     CDS             complement(3492..3830)
                     /locus_tag="U2R41_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890105.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3809..4228)
                     /locus_tag="U2R41_002772"
     CDS             complement(3809..4228)
                     /locus_tag="U2R41_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890106.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="U2R41_002773"
     CDS             complement(4221..4361)
                     /locus_tag="U2R41_002773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890107.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DASHWH010000134         4341 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.142, whole genome shotgun sequence.
ACCESSION   DASHWH010000134 DASHWH010000000
VERSION     DASHWH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.142"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="U2R41_002774"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="U2R41_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEM8890108.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="U2R41_002775"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="U2R41_002775"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEM8890109.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="U2R41_002776"
     CDS             2591..3376
                     /locus_tag="U2R41_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8890110.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4183
                     /locus_tag="U2R41_002777"
     CDS             3389..4183
                     /locus_tag="U2R41_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEM8890111.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
BASE COUNT         1497 a          705 c          935 g         1204 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaat taacgattat cggattattg
     4201 ctgatcgtag taggaagcat ggtcacggtc tttatttgag gataaagatg gtaaaatctc
     4261 atttattaaa aaaatatttg tttgaaccaa acagtaagta cttataaaaa aatagcgcta
     4321 gaatttccaa catctctgga a
//
LOCUS       DASHWH010000135         4218 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.143, whole genome shotgun sequence.
ACCESSION   DASHWH010000135 DASHWH010000000
VERSION     DASHWH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.143"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            10..210
                     /locus_tag="U2R41_002778"
     CDS             10..210
                     /locus_tag="U2R41_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEM8890112.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="U2R41_002779"
     CDS             288..962
                     /locus_tag="U2R41_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8890113.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            955..1119
                     /locus_tag="U2R41_002780"
     CDS             955..1119
                     /locus_tag="U2R41_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890114.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            1195..2337
                     /gene="dcm"
                     /locus_tag="U2R41_002781"
                     /pseudo
     CDS             1195..2337
                     /gene="dcm"
                     /locus_tag="U2R41_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2327..2617
                     /locus_tag="U2R41_002782"
     CDS             2327..2617
                     /locus_tag="U2R41_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890115.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="U2R41_002783"
     CDS             2876..4060
                     /locus_tag="U2R41_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8890116.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4057..4194
                     /locus_tag="U2R41_002784"
     CDS             4057..4194
                     /locus_tag="U2R41_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEM8890117.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DASHWH010000136         4197 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.144, whole genome shotgun sequence.
ACCESSION   DASHWH010000136 DASHWH010000000
VERSION     DASHWH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.144"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1..1221)
                     /locus_tag="U2R41_002785"
     CDS             complement(1..1221)
                     /locus_tag="U2R41_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEM8890118.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1241..1852)
                     /locus_tag="U2R41_002786"
     CDS             complement(1241..1852)
                     /locus_tag="U2R41_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8890119.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1895..2827)
                     /locus_tag="U2R41_002787"
     CDS             complement(1895..2827)
                     /locus_tag="U2R41_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM8890120.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3194..4108)
                     /locus_tag="U2R41_002788"
     CDS             complement(3194..4108)
                     /locus_tag="U2R41_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8890121.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLINEGKNTYFCYANFEKIHQR"
BASE COUNT         1191 a          849 c          692 g         1465 t
ORIGIN      
        1 ttattttaca gaagcactcg aaactgcatc tactgcagtt tcatcgtgtg tgctggcgct
       61 ggttactgca tctgtttttc caccatgaac agatgcactt gaaaccgcat cttcatttcc
      121 aacaagttgt tgacccgttt gttgtaaata ccactcaatg attggtttga aatcaagaat
      181 gtattcattg attccagcat actctccatt ttcatcatgc attgcttggt aattatgcac
      241 aatatatttt tctggcactt gttttggtac atgaacacgt accgcatctg ttgctccgct
      301 tcgcagttgt tgaattaccc actctacatt tttatacgat ttttctgggt gacagttggc
      361 taatggattc cctacctgac cgggcacacg agcagctaac atctctttcg cttcttggcg
      421 caaattataa tataaaaatt ggttattatt atctgcaaaa gttaattcca tcggcatcga
      481 atctaaaaag taattgattt gatccactgt caataaccca cgatttaatt tgacgtaagt
      541 atttccagaa acggcaccga ctgcttcaga agctttttcc aaccaatgat catctgacat
      601 atcaacatcg ggtattgtcg tagcaattgt tcctgtcgca tgcaaatctt ctggtggaag
      661 ctttttaggc cctgctaagg tagacacgac tttaatgaat tttacaaaac ttgctaatgt
      721 actgcctaaa aagtcaacag ttcgtggatc aatcaagcga tcttcttggt caaatgcttc
      781 ttttgctttt cccaataaaa attcatttcc gggcatcact aatgcattca cgcctggtgc
      841 gtccaaaatt tggcgcaagt gtaattgcgc gcgtgaagtt ccttgatcgt agtaagaagc
      901 acccacaacc attacaggtt tattttcaag tggatggatt ttaaatgata gccattcaag
      961 tgcacttttc aatcctgctg gaaccgtatg attgtgttct ggcgtagcaa taattacccc
     1021 atcagccatt aagattttgc gatttagctg ttgaatagca ggactatttg tttgatcatc
     1081 atcttgattg aacagaggta aatctttgat ttcaatgatt tctaaattaa atagttcatg
     1141 atattgtttt tggataaatg ttaataattt acggttatat gaaagttcag ctgctgatcc
     1201 gacgatacct attaatttca tctttgtttg ctcccctcga ttaaatcgtt tcccatgaaa
     1261 agctttcggc ttcacgttta tttgcttggt gcgcatgttg caattgacta gtgattgtga
     1321 caaagagtaa gaaatctttg aatagttcac gtaattttgc gacttgttct tcatcaatga
     1381 gtcgtccttg ctcatcaaag gcttggagtg aatgacccaa taaaaattct gagcttggca
     1441 taattcgtgc ctttaactct ggtgaatcca atatttggcg caaatgtgct tgtgcgcgtg
     1501 aagaacctaa cgtgccataa gacgctccgg taatcatcac tggtttatcc acaaacggaa
     1561 aaataccata tgaaagccaa tttaatgcgt taatcaatga agcaggaacc gcatggtcat
     1621 actctggtgt gctgatgatt acgccatctg cttgttcaat ttttccagca atttctttaa
     1681 caacatttgg tagttctgtg ttttcaggtt tgttaaatag tggcagctcc ttgatttcga
     1741 caatttcgat ttctgcctca tttacaaaat aatttttcat aaactgtaat aattcacggt
     1801 ttgttgaccg cgttgaattt gttcctacta atccaataaa tttcatcttc attctctttc
     1861 ctttctaaca tcgatttctg actgttcgca cgtactaaat tttttcaaat ccttttgtat
     1921 acaagaattg attctctttt gttacaactg ctccctcgat acccggctgt tcttcaagcg
     1981 ctttcaacac ttctaaagaa gatgctccaa ataaacgtgt agtccagatt tctccatcta
     2041 cagaagaatc agacaaaatc gtcaggcttg ctacgtcatt tttaactgga aagccagttt
     2101 tcggatcaat gatgtgatga tacactgacg aaccattttg tagataacgt tcgtagattc
     2161 cagaggtaac taaagattgg ttgcttagtt taaccaccgc aagcgactct tcacgtgaac
     2221 ctttaggatt tcgcaaacca atacgccaag caccatccga acgtcttggt gattcaccaa
     2281 aaacaacgat atttcctcct aagttaacta aagcaaacgc tgcatttcgc tctcggcaat
     2341 gtgtaattaa tttatctgca atgtaacctt ttgctaacgc ccccaagtca atcatcattc
     2401 ctggtttggt taaaaaaacg gttttcattt tttcatctaa ttgaatttgg tgtggatccg
     2461 ttagcagaag taactcagtt atctctgctt ttgttggaac caatgcatct tcaaatccaa
     2521 tgtgccattt ttgtacaacc ggaccaatag caatattcag ataactattt cttgcacaac
     2581 tatgtgcttt tcctaatttg atgagctcaa aaagttcagg atgtacaatc acagcttgtc
     2641 gtccagcagc ttgattgatg gccattaatt cagaagttgc atcatgtgcg ctgaagcgat
     2701 gttcataaac gtgcaaccat tcgcctaact ctgctagtaa tcgactaggc tcttcatgcc
     2761 caacttgtaa tgtaattgtt gtccccatca tttgccagat tttcgtttct tcgatcacct
     2821 gattcatttt gtaccttctt tacttgaact acggattaca tgagtaatta gtttttattt
     2881 ttgttactgt tttcacaacc agatgatacg cctgctagat ttatttgtca accgatttta
     2941 tatatttttt tatataataa ggtaatttta atataaaatt ttatattgta aaaaaacaaa
     3001 taagggaaac aaacaacaca tttttgtgtg ttgcttattt ccctctaact atcgttttct
     3061 atttgatggt taacctactt aaaataaata ctgcaacgac aaacggagat caagcatgct
     3121 ttgtaacgta ttttacgaga attacgactt tattttatta ctctatcttg ctgccatacc
     3181 cgtctatccc taatcatctt tggtgaattt tttcaaagtt agcataacaa aaataagtat
     3241 tttttccttc gttaatcaat agtatcattt ttttcatatg atttaattca tcaattaatt
     3301 ttttgttttt attaatttta gcaaaagcta gtgcatcttg aatcaattcg agcacttctg
     3361 ccttcgactt atttttagcc tgtgcgttct tcgctaattg ggcagcagct cgtgctaagt
     3421 aataagtcaa atggttatcc gaacaaatat cgattgcgta atttagtagt gcatcacttg
     3481 tttccaaatc accgtcttgc gcaaaaaaaa caccacaatg aaagacgata tttaacaccc
     3541 gccagatatc ttctgttgta cgaatcggat acgtataaat tttttcaaac actcgattga
     3601 aataaacttc tgctttttcc aactcttttt ctctagcata gaccattcca atacctgtat
     3661 atgccaataa acgaaacaac gtgttggctg tttctgccat caacaactga tcaaaatgga
     3721 acaacacatc cgtaattggt gcattcgtaa aaatcatttc aaatcctttt agatagtagt
     3781 aacgtgcaat cagctcagac tcgtcaatta ccttgggatc aatggctgct aaaatctgtg
     3841 tcaattgttc atattcactc gtaatcagtg aaaactctgc tttttctagc tgttcatcaa
     3901 gttgtgtatt gatcacgttc atcgaagaaa aaagttcaac taacggaaga ttgattcgat
     3961 tacataattt gattaaaatt tttaacgaag ggacttgacc attgttctca aatttactta
     4021 atgttgcttg tgtacaaatt ccttcagaca cttctttttg agataagttg gcagcctttc
     4081 ttgccgtaat aaatttttca atgttcatta ttttctctcc aatcagacct cacacgaaaa
     4141 tcatttctgt ctcgatttta gcatgttttc tttgggaatg ctttaattgg ttaagat
//
LOCUS       DASHWH010000137         4158 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.145, whole genome shotgun sequence.
ACCESSION   DASHWH010000137 DASHWH010000000
VERSION     DASHWH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.145"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            177..1526
                     /locus_tag="U2R41_002789"
     CDS             177..1526
                     /locus_tag="U2R41_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8890122.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLTQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1654..2244)
                     /locus_tag="U2R41_002790"
     CDS             complement(1654..2244)
                     /locus_tag="U2R41_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM8890123.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2391..3743
                     /gene="brnQ"
                     /locus_tag="U2R41_002791"
     CDS             2391..3743
                     /gene="brnQ"
                     /locus_tag="U2R41_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM8890124.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1201 a          757 c          807 g         1393 t
ORIGIN      
        1 aatgcttagg atcaatagct gattgggaca aagagctgcg gtgtttaccg cagttctttt
       61 ttatgaatta ctcataaaag gtaaatttct gaaaattttg gaatcttagt tgtgtttttc
      121 agaaaactct cttaatatgg aagtaattaa acttttttag ttaggatgat ttaaaaatga
      181 atgaagagca agaactgcag cgtggactga aaaatagaca tgtccaactg atttctattg
      241 gcggagcgat tggaacgggg ttgtttttag gttccggaaa gtctatccag ctagctggcc
      301 cttcgatatt gctcgcctat cttattactg gatgcatctg ttttttcatt atgcgtgcgt
      361 taggcgaact tctgttatca aacaccaata accattcttt tctggatttt gttgcagaat
      421 atcttggcaa aaaagcggct ttcatcactg gctggacgta ttggttctgc tgggtatcga
      481 ttgcaatggc cgatttaaca gccatcggaa tgtatgtacg ctactgggcg cctggtgttc
      541 ctcagtggct tcctgaactg attgcattag gtcttcttct ttgcttgaat atggtggctg
      601 tctcattgtt tggtgagtta gagttttggt ttgcattgat caaagttgta gcgatcattg
      661 cctttatcct cgtaggagct tatatgatcc tcacccacta tacgacagat attggcacag
      721 ccagtatcac gaatctatgg tctcatggcg gattctttcc aactggtgca aaaggattta
      781 tcttagcttt ccaaatggta acctttgctt ttgctgggat agaattagtt ggattagtcg
      841 ctggagaaac agaaaatcct gaaaaagtgt tgccggaagc tatcaataat attcctatca
      901 ggatcatctt attttactta ggatctcttt ttgtcattat ggcaatctat ccatggaaca
      961 gcttgaatcc agataacagt ccatttgtag aagtattttc ggaaattggg atcacgattg
     1021 ctgcttcctt gatcaattta gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa
     1081 tatatagtac aggacgtatg cttcgttctt taacgcaaga aggcagtgcc cctaaaaaat
     1141 tcaaaaagtt aacaacacat catgtgccag ggaatgcttt gacattttct actatcgtca
     1201 ttttcatttc tgttatcttg aattacgtga tgcctagtga agtattcaca ctagtaagca
     1261 gtattgccac tacttgtttc ttgtttatct ggagtatgct tgtctacacg cacatgaaat
     1321 atcgtaaaag tatcctcggc aaaaaagctc attcatttaa aatgccgttg tacccattta
     1381 gtaattatct ggtatttctt tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag
     1441 atacgagaat cgctttatta ctaacacctg tgtggttctt gcttctatta ctaatctatc
     1501 atatgaaata tgaacgtaag aagtaagaaa gtagttgtga cagaagtggt cagcttcgag
     1561 aaataagaag gaatttacga aaattgcttc ttaaattttt gtgaaatttc ggcttatttc
     1621 ttggagataa aagcttctgt cccagcctct tttctatttc cccggaaaca accgctgata
     1681 ataggcatct gcttttttat aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa
     1741 taacccgatc aacggtacaa aaaagacacc ggcaatcaag cataagttaa tcatcatgga
     1801 gataagactt cgacggtcct ttgacaaaac tctcacgaca tgggaatctg gtttatttgc
     1861 tttcatgatt tgcctcatca agataccaaa agaaaaactc cacatcgtgt atactaagac
     1921 ataagccatc tcaggccaaa aagcttcagg gttacgcccg acccaactag taacagaagg
     1981 acataaagtc agccaaaaga gcaaaaagat attcgaccag accactcggt aattaattcg
     2041 ttctgccatc tgaaatatac gatgatggtt cacccagatc accgcaacaa atataaagct
     2101 gatggtataa gctaataatg tatttcggat gacccataaa gaatgaaagc tagatgtatt
     2161 agggatcttg atctctaata caataactct caaaacaatg gctaccactc catcagtaaa
     2221 tgcttctaat ctcgactttg tcacgatgat tcctcctcag atgattcagc gcttttattt
     2281 tatcacttac gttattaaat aaccatcgtt ttatataggg aattttctga aaattgttgc
     2341 atatttttcg aaattttgtt acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa
     2401 aattatcatt taaagactat ctttttatag gctccatgct tttcggtcta ttttttggtg
     2461 ctggaaatct tatctttccc gttcatttgg gtcaagaagc aggcgctgct accttttggg
     2521 cgaatctagg tttcttggtt accggtatcg gtcttccctt cttaggggtg attgccattg
     2581 gtgtatcgaa aagctcaggt gtatatgaat tagcaaaacg gataaatcaa acatatgcga
     2641 tggtttttac catcttgctt tacttagtta tcggaccgtt ttttgcttta cctcgccttg
     2701 caaccacatc tttcgagatt ggtttagcac catttatcga tcctagcaga cagacgctat
     2761 atctagcaat tttcagtatt gtattttttg tgctggcttg gtggttttca agaaaaccga
     2821 caaagatatt agattatgtt ggaaagttcc tgaatcctgc atttttggtt ttattaggtg
     2881 ctttgcttct ccttgctttt ttgaatccac taggttctgt aaatcaagcg cctattcagc
     2941 caaattatca agagcatgct tttttcacag ggtttactca aggatataac acattagatg
     3001 cgcttgctgc tttagcattt gggatcatca ttgtcacaac aatccaaaac atgggtgtaa
     3061 caaaacctgc tgaaatcgct aaagacacga tcaaatctgg tgcgatcagc attatattga
     3121 tgggaatcat ttatactttg ctcgcttatt taggtgctat gagcttaggt agctttgctc
     3181 taagtgaaaa tggcgggatc acgctggctc agattgctga tcattattta ggtacttatg
     3241 gcagtatctt attagcattt atcgttatat tagcttgctt gaagaccggt attggtttga
     3301 ttacagcttt tgcagaaaca ttcacagatt tgtttccgca aagaaattac gcattctttg
     3361 ttgcacttgc tagtatcctt ccttgtttag cggcaaacgt aggcttgacg aatatcattc
     3421 aattttcgtt gcctgttttg atgttcatct atccattagc aatgacactg attttactag
     3481 tgttagttgg tcctttgttt aagcagcgta cggctgttta tcgaatgact acttatttta
     3541 cattgattgc ttcgattttt gatgggttga acgcttgccc agaaactatc aaacaaacac
     3601 ctattgttca aaatctttta tatgttgcgg aaagctactt gccgtttttc aaattgggga
     3661 tggggtggat cgtaccagca gttatcggtt ttgttatcgg actgatttgg agtttcgcta
     3721 agaaagaaga agtagcagat tgataaaaga ggttgtgaaa gagacgttca gccttgagca
     3781 ataaaaagta attttgaaaa atggcttctc acatttttgc ataaatttga cttaataccg
     3841 agaaactgac ttttgaacac cgtttattga gaggtcgtga aaaagccgct tagtttcaag
     3901 tattaagaaa tattttgtga aaatggcttc tcacattttt gcataaattt gacttaatac
     3961 cgagaaactg gcttttgaac accgtttatt gagcgtctat aaactgacct gagtattaga
     4021 gaaacaaaac ggcaaagtag ctccatatac tttgccgttt tgttttgaac taataccgaa
     4081 gagaagctga tcaaacaaca tttcttttat aatatagatt ttagacatat atatgatata
     4141 aaaatagttt tcgcaaaa
//
LOCUS       DASHWH010000138         4034 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.146, whole genome shotgun sequence.
ACCESSION   DASHWH010000138 DASHWH010000000
VERSION     DASHWH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.146"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            73..234
                     /locus_tag="U2R41_002792"
     CDS             73..234
                     /locus_tag="U2R41_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEM8890125.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="U2R41_002793"
     CDS             253..1530
                     /locus_tag="U2R41_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8890126.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="U2R41_002794"
     CDS             1540..2196
                     /locus_tag="U2R41_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8890127.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="U2R41_002795"
     CDS             2196..3572
                     /locus_tag="U2R41_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8890128.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="U2R41_002796"
     CDS             3702..>4034
                     /locus_tag="U2R41_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8890129.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          439 c          704 g         1279 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DASHWH010000139         3887 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.147, whole genome shotgun sequence.
ACCESSION   DASHWH010000139 DASHWH010000000
VERSION     DASHWH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.147"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(26..478)
                     /locus_tag="U2R41_002797"
     CDS             complement(26..478)
                     /locus_tag="U2R41_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890130.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(943..1311)
                     /locus_tag="U2R41_002798"
     CDS             complement(943..1311)
                     /locus_tag="U2R41_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEM8890131.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            1461..1943
                     /locus_tag="U2R41_002799"
     CDS             1461..1943
                     /locus_tag="U2R41_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEM8890132.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1980..2990
                     /locus_tag="U2R41_002800"
     CDS             1980..2990
                     /locus_tag="U2R41_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEM8890133.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3105..3818)
                     /locus_tag="U2R41_002801"
     CDS             complement(3105..3818)
                     /locus_tag="U2R41_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890134.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1288 a          631 c          733 g         1235 t
ORIGIN      
        1 ggaatttcta gcgcttttta attgtttaag atacaagtga aatatctgtc tgcatcaata
       61 atttttcatt gatccattga ctaaaattta cgacatgatt gaagttacca ccgattagta
      121 tctccgcatg attgtcttta taaagatcgt attgtaaaag acggtctttt ttatttgcgt
      181 aaacatcgtt atggtactgg actaattgtt ctcggatttg attgattgct gtaaaatcac
      241 gatactgaag gttgaaacgc atcgtgattg gtgcatttag catgaatcgc tgcatttctt
      301 ttgctgcttc ctctgcttga tctccgtata ggaactcaaa aagattacgt tcagaaaatt
      361 tccattgccc gccaaccttt gttcctttta gttttccttc atgaagataa tttctgatag
      421 aacgtgtagt aacacctaat tgttcggcta attcatttac tgagtaaaaa tctttcaatg
      481 ttcaccctcc tatgtgaatg gttctactga cttagttgat atttgcttaa tcacgaaatg
      541 tagaaagaac gattttaatt aaggtcattg taaaaatcaa gtcagaaaat tctaactttt
      601 ctttctctaa agaaattcgt tctctcctaa agtgatatta tatttaaata tacctgaaaa
      661 taatgggcag tattatgaaa gatcactgat attttctcgt attttttcct tattcaaaag
      721 ttttgcagga aaattgctga aaaaataatt ttactaagtg gaaaattcaa agccattcaa
      781 tcagtgattt ttttcagcac aagaaagact ccttataaaa atagcagata ctttgtcaat
      841 taagtgttta accagggatg ataaaaagga taggattgaa caaaacctgt ataagctgat
      901 gaatagtttc ctcgccagta aaaggtttat tcgtataaag ctttatcgcc aaaatttatc
      961 atgacctttt ttatcttcaa tattaaatga aatcaattgt tcaatcaatt cccagttgat
     1021 tacacttttc caaggaactt tgatgacgtt atccgtatat acgtagcctg cttcttggat
     1081 atttttttca agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat gatttttagc
     1141 aaaactaaat gcaataataa aggtaccatg gtcagtaaac ataggttggt tccattttac
     1201 agttgtgtct agctggggat acgtttctgt tatcttagca aaaaggtctt tgatcttttg
     1261 ttgatgttct ttttctttga tctgattgat gatcggctga aatttttcca ttctaaaacg
     1321 cctccattaa gtggtttgat ttattttacc agaaaaacaa tagttatgta taaaaaacga
     1381 acaaaacaca agtaatgttg taaccgctat atttatatga taagatgtct caaataataa
     1441 aatagaaaag agacataaaa atgattgact tggcaaagaa attcttacac gaaaatatat
     1501 attttgagtt gtatcatcat cgagccattt atacaaatga agatgctttg gtaataaaga
     1561 aagaacaagg atttacaggt acagagacca aaagcttatt cttaaaagat aaaaaagata
     1621 ataactacgt atatctgact tttactacga aacaaacgga tttcaaagca ttagggaaga
     1681 tcgtcggtaa aaggctttct attgtttcat cagaacgcat ggaagaacaa acgggacaaa
     1741 agcagggcgc tgtttctccg tttggttatg aaaaacagat tccgattatt attgatgaag
     1801 agctcttatc aaatgaaaaa cttgtttttg caccaggtag accagatcaa acgatggtca
     1861 tccaaacggt tgacttaaac aaaatcatcc agctacttga gatgccgata tttttgatgc
     1921 cggcaaacga aaaaaacaat taaccaagta aaatagtgca tacttggagg tgaagcaaca
     1981 tgataagtta tccattatta gaaagaaatg caacgattgg ggttactgca ccatcctcag
     2041 gcgtatcaga ggaacaccat tcgttactcg aatcagcttt aaagagaatg gaagaaaaat
     2101 ctttccaagt agttactgga aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag
     2161 caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct
     2221 ttccgccttg gggaggagaa ttattgattg agattctcga ttttgtcgat tttgatgctc
     2281 tacaaccaaa atggatagta ggttattcag atattagtac gttgctactt gctgtcacat
     2341 taaaaacagg gatagccact gcacatggaa ccaaccttat tgatttaaga ggagagtact
     2401 tggatgaaac gacagcaatg tgggaaagtg tactgaaaac aagagaagga gaatcgattc
     2461 tacagcaacc ttctgaaaag tatcaaaaag agtggcccca tgagcattca tcatcttctg
     2521 ttttccatct cactgaacca acaaattggc aatcagtcag cggagatgca gtaaggatga
     2581 agggacgatt attaggtggc tgtattgata cgatcagtca tttaatcggt accccttatg
     2641 gagatgtcca atcttttaaa agaaagtata atgatggaaa gccgatcttg tggtttctag
     2701 aaaattgtga gctatcgtca gttgatttaa gaagatcact tgttcagatg aaactggcag
     2761 gatggtttga tggcagctcg gggatcatgt tcggtagaag tgcagcagag gatttgttag
     2821 attataaaat gaaggatgta tatgaagatc tgtcaaagga attaaaacta ccgatcttgt
     2881 atgatattga ttgtggtcat gtaccgccac aactgacatt gatcaatggt gcctatgcag
     2941 aagtagcggt agaagataga aaagcgactg ttttgcaaac gtttatttaa aaaatttttt
     3001 cctgccttgg agtttatcaa agaccaacaa atctaaatag aaaagaaaat gaaagaagct
     3061 gacagtattt tgccagcttc tttttgtatg atctattaga aattttaatt atccataact
     3121 atcacattga tcagctgttt cacatcattt ggattacctg taggaatagc tactacagat
     3181 gcattatcaa taatttcttg agttgtttct ctaatgaacg ctggataaga tgtttgatct
     3241 gcaggcattt gccaatgtga aggaactgga taactagtga ttgtcccgtc atggttactt
     3301 gaatacacta aaactccctg gtaactgtat ttcccagtga gcataaccac atctttcgga
     3361 tagactgcac tccctgcatc ataaggggcg ataggtgttc ctgcaggata atgaataaca
     3421 tgtagttcga aatcacccga tgtatctcta aaagtattac ctctaattgc tagccaaaca
     3481 cgtgcgtact caatttgtag atcagaatat cctgctaacg gatctattga ctgagaagac
     3541 tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt actactagat
     3601 gtagaagaag tattgggctc tgagacttta ctgcttgttg aagatgtatt cgtttcagta
     3661 gaagctgatt tctgagtact tgatttttca gctgaactgg tttgtttgat tgaacttttg
     3721 tctgtggtcg cagatggatc actattttct ttactaggag acgtacatcc tacaaggaat
     3781 aaagagacca acaataaaat aaaacctaac tttttcatat aaaatacctc attatttttt
     3841 taataccagt atagcataat cctgaatttt tgaatccgta aaacctt
//
LOCUS       DASHWH010000140         3878 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.148, whole genome shotgun sequence.
ACCESSION   DASHWH010000140 DASHWH010000000
VERSION     DASHWH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.148"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(718..3867)
                     /locus_tag="U2R41_002802"
     CDS             complement(718..3867)
                     /locus_tag="U2R41_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM8890135.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DASHWH010000141         3857 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.149, whole genome shotgun sequence.
ACCESSION   DASHWH010000141 DASHWH010000000
VERSION     DASHWH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.149"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            148..1128
                     /locus_tag="U2R41_002803"
     CDS             148..1128
                     /locus_tag="U2R41_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8890136.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIVEEKQYQFIF
                     MTLTTPNVKADKLNEEIDLFNHALSKMFRRKRVTNVIKGYVRKLEITYNKKRDDYNPH
                     FHLILAVPKHYFTVKKYYIKQSEWLDLWRDVTGKTGINPDGTDEITQLDVRKVKGFQQ
                     EKAVQEVAKYSAKDFEMTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYEAGELDHY
                     KKKDENEYFWFLTASWMQKDANYSVAVRELTDDEKKFFAERYDKEGDETG"
     gene            1100..1363
                     /locus_tag="U2R41_002804"
     CDS             1100..1363
                     /locus_tag="U2R41_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM8890137.1"
                     /translation="MIRRVMKLDKQRLYEAVKDNCNCGGNRQLFKAMMVTVYRRLDGE
                     NAEAAVENAYFQVFGEHHSPEWDRAILDDMAEMHEKLWRALND"
     gene            1416..1778
                     /locus_tag="U2R41_002805"
     CDS             1416..1778
                     /locus_tag="U2R41_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890138.1"
                     /translation="MGVTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            2000..3406
                     /locus_tag="U2R41_002806"
     CDS             2000..3406
                     /locus_tag="U2R41_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8890139.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSENEIEW"
BASE COUNT         1306 a          553 c          928 g         1070 t
ORIGIN      
        1 aaaaaaaaag attgcttttc caatatggtt gatttatagt tttagttgac taaaaaaaac
       61 taaatcagaa tggagagcaa tcttaatttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccagaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagcc
      421 attgtggaag aaaagcagta tcaatttatt tttatgacgt taacaacgcc taatgtgaag
      481 gcggacaagc taaacgaaga aattgattta tttaatcatg cgctatcgaa aatgtttcgt
      541 aggaaaagag ttactaatgt aatcaagggg tacgtccgga aattggaaat cacgtataac
      601 aaaaaacgtg atgactataa tccgcatttt catttgattt tggcggttcc taaacactat
      661 ttcactgtca aaaaatatta tatcaagcag tcggaatggc ttgatttatg gcgtgatgtg
      721 actgggaaaa cgggaattaa tcctgatggc acagacgaaa taactcagtt agatgtccgt
      781 aaggtcaaag gattccaaca agaaaaagca gtacaggaag ttgcaaaata ttcggcaaaa
      841 gattttgaaa tgaccgagag tcaagaagtg tttgacacat tctatcatgc tatgaaaggg
      901 cgccagttaa tcacgtttaa cggggtcttc aaggagtaca agaagaaata cgaagcaggc
      961 gagctcgatc actacaagaa aaaagacgag aacgaatatt tctggttttt gactgctagc
     1021 tggatgcaaa aggatgccaa ttactcggtg gccgttcgtg agttgacgga tgatgagaag
     1081 aaattctttg cggaacgcta tgataaggag ggtgatgaaa ctggataagc aaaggctata
     1141 tgaagcggtg aaagataatt gtaattgtgg cggaaatcgt cagcttttta aggctatgat
     1201 ggtgactgtt tatcgtcggt tggatgggga aaatgctgag gcagctgtgg aaaatgctta
     1261 ttttcaagtg ttcggtgaac accatagccc tgaatgggat cgtgctattc ttgatgatat
     1321 ggcggaaatg cacgagaaat tgtggagggc gctaaatgac taatcaaatt ggtgttattt
     1381 ataggaatga atctaagggt aaggagttgt aattcatggg agtaacttta gctgatatta
     1441 aagcacaaga agaaaaattg aaaaaagagc aggcaaaact tcgccaacag aaaaagcgat
     1501 tgattgagaa ggaaaatgct gaaatgggca aggcgcttcg caagcatttt gggtttgaat
     1561 cttttgctga ttttgagcgg tggctatcgt cgatttctga cgatcagcaa cacgaaaaat
     1621 cagtggataa tgagtggctt gctgatgaaa aatcagcgcc aagcgaacga gattatcccg
     1681 atgaatctga cggcttattc ggagtgatac cgaataagaa aagtcagatg agtgagagta
     1741 aatttacgtc aatatttaat gacgtgaatt tacgataagc gattattttg cgtgaagcaa
     1801 aataaagcgt aaaaggagtt gagagcgtga tacgctttcg gctccttttt tcgttgtcac
     1861 ttgtgacagc gaaatccctc ggagagcgtt tggaattgtg acagtcatgt cacaattagg
     1921 aaaacgttac tctttgacaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1981 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaagaatac
     2041 aagcatttct cataataatc gtgagttaac tgatagccaa aaaatcaatg actggcataa
     2101 gcatattgat ttcacaaaat cggatgaaaa tatctatttg gaacaaacaa atattcgtga
     2161 aaaatatgaa gaattattcg gtgaagccgt agaaaaatat aatgcaaagc agaaaagagc
     2221 cgatagaaaa atcgaggact atttagcaaa ggttcgcaag gataaaaaat tggaaccaca
     2281 gcgagaattt atcgtgcaaa ttggtactct tgatgatttt cgtactactc gtgatgatgg
     2341 ttcttctact ggcatatcgg aacaacaagc agaacaaaat cgagtgattg ccaacaaaat
     2401 tttagttgag tatttcaaag aatttaaaga acggaatcct aatttatccg tctacaatgc
     2461 tgttattcat aatgacgaag taagtccgca cctgcatttg aatattgttc ctgttgctga
     2521 agggtataaa cggggtgttc aaaagcaacc aagttttaac aaagccttgc aacagcaggg
     2581 tttacgagca gataaagaga atggtcgagt gatttggaat aattttagga atcaggaagt
     2641 tgagtcaatt gaacgtttga tggcagattt tggttgggag cgtgagttag tcggtactaa
     2701 caaaatcaaa gatgttcacg aatataaaga aatcatgagc gagatttcag aattgcgtca
     2761 aacggccgta aaagagcgtg agagcgtttc tgatgagttg gggtatattt ctaggcaaag
     2821 agaagaaaat gagcgagaaa aggctctggt ggcttctgag agagcgaaga ttgaagaaga
     2881 gaagaaaaat atgctgaatg taaaaaaaga gatggcggtc attttccaaa aagatattgt
     2941 tcctttagaa agtttcaaga ggcttcctga tggttcgtat catttatctg ggaatgattt
     3001 tgttgatttg tatcaacgag ctacgagagc aacaaatgcc aagagaaatg ctgataacat
     3061 gagtgagcaa tatttacaga tgcttgagca aaatacagaa ttaaatgagt atgtagctgc
     3121 attggaagaa gagcgtcatt tgtctggtag aaaacaatta gcagatttac aagtggaaaa
     3181 taaaaagctg aaagaagaaa atcgctcttt acgccagcgt ttagagcgat taaaaaacgc
     3241 atttcaaaaa tcgatcactc gtttgtctat ccgttttggt cttgcagaaa aggatatggg
     3301 gctttctgag gaattagaag tattagagca ggagaaaacc ttattttatc gtcaaaacgt
     3361 ctcacagcgt aaattagagc gttctgagaa tgagattgag tggtagccac gatgtgcagt
     3421 caacggtacc ggaggcggtt tgtgccgagg ataaggagtt gacggctaaa ccactagcct
     3481 attaagctga catttgctaa aaaatggcca gtccattttt tggccaactt agcgttagcg
     3541 aagtttgggg gcgccctttt taaagggtgc ggttttatgt ttgcatgact aagaatgagt
     3601 gggaaaaact taaccgcatt tctaacaggc ggttaaatgt cggtgagagc gtaaaaatga
     3661 taaaaatgtc ttggtcgatt gaccaaattt tatgcagaag tgggtttttg agttttgggg
     3721 tttgaaacga gaaaaaagag gtcgatttcg acctcttttt ttgatgtttc ttcttatctt
     3781 gatacataag gtaactattt tcggcagaaa atcggggcta tagtatgggt ctatcccttg
     3841 tggctctaag ggagaac
//
LOCUS       DASHWH010000142         3810 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.150, whole genome shotgun sequence.
ACCESSION   DASHWH010000142 DASHWH010000000
VERSION     DASHWH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.150"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            97..2196
                     /locus_tag="U2R41_002807"
     CDS             97..2196
                     /locus_tag="U2R41_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM8890140.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2392..3255
                     /locus_tag="U2R41_002808"
     CDS             2392..3255
                     /locus_tag="U2R41_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM8890141.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3360..>3810
                     /locus_tag="U2R41_002809"
     CDS             3360..>3810
                     /locus_tag="U2R41_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8890142.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1522 a          636 c          772 g          880 t
ORIGIN      
        1 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
       61 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
      121 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
      181 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
      241 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
      301 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
      361 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
      421 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
      481 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
      541 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
      601 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
      661 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
      721 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
      781 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
      841 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
      901 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
      961 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
     1021 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
     1081 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
     1141 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
     1201 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
     1261 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
     1321 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
     1381 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
     1441 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
     1501 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
     1561 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
     1621 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
     1681 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
     1741 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
     1801 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
     1861 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
     1921 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
     1981 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
     2041 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
     2101 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
     2161 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
     2221 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
     2281 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
     2341 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
     2401 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
     2461 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
     2521 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
     2581 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
     2641 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
     2701 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
     2761 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
     2821 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
     2881 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
     2941 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
     3001 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
     3061 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
     3121 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
     3181 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
     3241 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
     3301 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
     3361 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
     3421 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
     3481 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
     3541 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
     3601 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
     3661 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
     3721 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
     3781 atggaatctg tcaggaatgc tatgaaaaag
//
LOCUS       DASHWH010000143         3785 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.151, whole genome shotgun sequence.
ACCESSION   DASHWH010000143 DASHWH010000000
VERSION     DASHWH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.151"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..171
                     /locus_tag="U2R41_002810"
     CDS             <1..171
                     /locus_tag="U2R41_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8890143.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="U2R41_002811"
     CDS             406..642
                     /locus_tag="U2R41_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890144.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="U2R41_002812"
     CDS             649..2601
                     /locus_tag="U2R41_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEM8890145.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="U2R41_002813"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="U2R41_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEM8890146.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="U2R41_002814"
     CDS             3400..>3785
                     /locus_tag="U2R41_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8890147.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DASHWH010000144         3628 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.152, whole genome shotgun sequence.
ACCESSION   DASHWH010000144 DASHWH010000000
VERSION     DASHWH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.152"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            70..987
                     /locus_tag="U2R41_002815"
     CDS             70..987
                     /locus_tag="U2R41_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEM8890148.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            1000..2144
                     /locus_tag="U2R41_002816"
                     /pseudo
     CDS             1000..2144
                     /locus_tag="U2R41_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            2315..2773
                     /locus_tag="U2R41_002817"
     CDS             2315..2773
                     /locus_tag="U2R41_002817"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEM8890149.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            2932..3402
                     /locus_tag="U2R41_002818"
     CDS             2932..3402
                     /locus_tag="U2R41_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEM8890150.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(3455..3570)
                     /gene="rrf"
                     /locus_tag="U2R41_002819"
     rRNA            complement(3455..3570)
                     /gene="rrf"
                     /locus_tag="U2R41_002819"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1178 a          694 c          701 g         1055 t
ORIGIN      
        1 tctatgtctt tcaaggagct gatttaatag tcagctcctt tttttattat ctttttttag
       61 gaggcccaca tgattatctc gacaattaca gaagctattg gttccacacc gctttatcgc
      121 tttgacaatc agcactattc tatgcctaaa ggttctgcta tttatgcaaa actagaatat
      181 ttaaatcctg gaggaagtat caaagataga ttgggcagtt atttgatcaa agaaggattt
      241 gcccaaggaa aaatcaatga aaaaacgacg atcatcgaac caactgccgg aaatacagga
      301 attgggatcg ctctggccgc actcacatac aaattaagaa cggtttttgt tgttccagag
      361 aagtttagct tagaaaaaca acaattgatg aaagcattgg gagcaaagat catccattct
      421 cctagtgagc aaggaatcat cggtgccatc agcaaaagta aaaaacttgc agaggagata
      481 tcaaacagct atctgcctct tcaatttgaa aataaagata atcctgctgc ttactatcat
      541 acgttagggc cagaaatctt tcatgaaatc aaagaaaata tccatagttt tgtagcagga
      601 attgggagtg gtggaacctt tgctggaact tctacctttt tgaaagaaaa atatcccgac
      661 attcgaataa tcggtgtaga accagagggc tctgtgttaa atggtggtga tccagcccct
      721 cacgaaattg agggtatcgg tgtagaattc attccgccgt ttctttctcc cctttcaatc
      781 aatcagatcg agacaatctc tgatgtagaa gggtttaact atactaggca gttagcacga
      841 gaacaaggac tattagttgg aagttcgagt ggtgcggctt ttgcagcagc attaagagaa
      901 attcgtcgtt taccacctgg tcatcgtgtc gttacgattt ttccagacgc agcagaccgc
      961 tatttatcaa aaaacattta cttataggag gaaagaaaca tgcacattca aacaaaattg
     1021 atccacggag gcatcagtga agatcccaca acaggagctg tcagtgtacc tatttatcaa
     1081 acatccactt atcggcaaga tggcgtcggc cagcctaaac aatatgaata ttcaagatct
     1141 ggtaatccta ctcgttttgc attggaagaa ctgattgctg atttggaagg cggtgttcga
     1201 ggattcgctt tttcttctgg tctttctggg attcatgccg ttttttctct ttttcaagct
     1261 ggcgatcata tcttattagg agatgatgtc tatggaggaa cattccgtct atttgacaaa
     1321 gttcttacta aaaatggtct cgagtacacg atcatagata cgagtaatct tgataaaata
     1381 gaacaatcca tcaaacccaa tacaaaagca ctttatcttg aaacacctag caatccgctt
     1441 ctaaagatta ctgatttaga aaaaagtgcc actctggctc atcaacatgg attgatagtt
     1501 attgctgaca atacatttgc tactccttat tttcaaagac ctttagatct cggttctgat
     1561 atcgttgtcc atagcggtac caaatatcta ggcggacaca gcgatgtagt agctggttta
     1621 gtgacttcta accaaaagat ctagccgatc agatcggatt ttaccaaaat gctatcggag
     1681 ccgtacttgg tccccaagat agctggttac ttcagcgcgg aatcaagaca ctaagtgttc
     1741 gtatggagga acatcaaaaa aatgcttttg tggtagctga ttttctattt tctcatccag
     1801 cagtagaaaa ggtctactat ccaggattac ccgatcatga actccatgga gttgctaagc
     1861 agcagatgag cggctttagc ggaatgattt cttttacttt aaaaaatgaa gaatctgcta
     1921 ttccatttgt tgaatcactc caactattta ctttagcaga aagtttaggt ggcgtagaaa
     1981 gtttagtgga aattccttct gtcatgactc atgcgtctat tccaaaagaa aaacgagaag
     2041 aggctggcat aaaggacggc ttgatccgtc tttctgtagg aattgagtat ggtcaggatc
     2101 tcataaacga tttagctcag gcatttgaca gaatcaagaa ctagaagtcc caataaacga
     2161 cttagcagaa aaatctaaaa aatatttcag attgctacac cttggaaacg ataagtgtta
     2221 gaatcaagta gaaagacgtt tttgtaaatt tcactaaata attctttagt gaaaagcatc
     2281 aaaacgaatg aacaaacaga taggagtctt acaaatgaga atgaatgtcg aaagttttaa
     2341 tcttgaccat acaaaagtca aagcacctta tgtacgtgta gctgatcgaa aaacgggtgc
     2401 gcatggcgat gtcatcatta aatacgacgt ccgctttaaa caaccaaata aagaacacat
     2461 ggacatgcca agtctacatt cacttgagca cttgactgcc gaactaatcc gcaatcacgc
     2521 agattacatc gtagactggt ctccaatggg atgccaaaca ggcttttact tgactgtatt
     2581 gaatcatgaa agctacgaag acattttaga tgttcttgaa gcaacaatga aagatgttct
     2641 tgaagctact gaagtacctg caagcaatga aacacaatgt ggctgggctg ccagccatac
     2701 actagaaggt gcacaagctt tggctcgtga atttttagca aaacgcgatg aatggtctga
     2761 agtagaagca tagtttctaa aagaacgtaa agatgagaag caatatagct atcttatctt
     2821 cgtattttat taaatattta ttgtctgaaa agagttctaa acgggaaaac aaatagctag
     2881 ttttcagttt agcgctcttt ttattatata aaatagaaag gagaaaactt tatgtctatt
     2941 tataattttg aaactacttt agaagacggc actgtctatt cattgaaaaa atatgcaggc
     3001 aaaccgataa tcattgttaa cactgcaaca aaatgcggtc tagcacctca atttaaacaa
     3061 ttagaagaac tttaccaagc ttatcagaca caaggtctgg ttatcttagg ctttccttcc
     3121 aatcaattta aacaagagct agattcagcg actgaagcag cagaagcttg taaaaccact
     3181 tatggcgtta ccttccctat gcatcaattg attgacgtaa atggagaaaa tacagatcct
     3241 ctttttactt atcttaaaaa tgaagctcct ggtactgtca ctaaagcaat aaaatggaat
     3301 tttactaaat ttttgattga tagaaatggc aaagtaatcg aacgatttgc accacaaact
     3361 tctccattga aaatgaccgc tgcaattgaa tccgttcttt aacttaataa aaataaaaaa
     3421 agatgaacta tttctagttc atctttttgc ctttgcgtgg cgacgtccta ctctcacaag
     3481 gggcaacccc tcactacaat cggcgctaag aagcttaact tctgtgttcg gcatggttac
     3541 aggtgtatcc ttctcgctat cgccaccaca ctgtggtgtt atcttttatt gagtaaattt
     3601 tgttcactca aaactggatt tgaagtca
//
LOCUS       DASHWH010000145         3448 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.153, whole genome shotgun sequence.
ACCESSION   DASHWH010000145 DASHWH010000000
VERSION     DASHWH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.153"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            115..975
                     /locus_tag="U2R41_002820"
     CDS             115..975
                     /locus_tag="U2R41_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEM8890151.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            987..1814
                     /gene="panB"
                     /locus_tag="U2R41_002821"
     CDS             987..1814
                     /gene="panB"
                     /locus_tag="U2R41_002821"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEM8890152.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1829..2677
                     /locus_tag="U2R41_002822"
     CDS             1829..2677
                     /locus_tag="U2R41_002822"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEM8890153.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2687..3082
                     /locus_tag="U2R41_002823"
     CDS             2687..3082
                     /locus_tag="U2R41_002823"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEM8890154.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3284..>3448
                     /locus_tag="U2R41_002824"
     CDS             3284..>3448
                     /locus_tag="U2R41_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890155.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT         1336 a          375 c          686 g         1051 t
ORIGIN      
        1 atattatgat aaattaacta tggatgatat ttgaaaaaaa catactgtat atttgattta
       61 atttttattt ttaagctcat atcgtaagaa tgggacttgt agtctcactt ctttatggag
      121 gtgataatta tgaacgttgg ttttattgga gccggcaagg taggttgttc attcgggaag
      181 tatttccagg aacataaaat acaagtaaca ggattttata gtaaaagcga agactcgtca
      241 ttagcagctt cgaattttac atctagtaaa caatatttaa acttaagaga attagtagat
      301 gaaaatgata ctattttcat tacgactcct gatggtcaaa ttcaggaggt atggcaagaa
      361 attaaaaatt atcaaatcaa aaacaaatta atctgccatt gcagtggtgc aatatcttca
      421 gatatctttt caaatataca ggactatggt gcttatggct attctgtcca tcctatgttt
      481 tcaatatcag ataaatataa ttcatacaaa aaattaaaaa aatcttttat aacaattgaa
      541 ggcgatgaaa aatatgcaaa gtttttatgt gatttcttca aaaagttagg taatagtact
      601 gtaattgtat ctaaggagaa taaatctctt tatcatgccg catcagtagt atctagtaac
      661 cttgtgctag gacttattaa tacaagcgtt acatatctaa ttcaatgtgg atttactgaa
      721 aaaatggcaa ttgaagcttt atatccatta attgaattta atattagaaa tataaaagaa
      781 aaaggaatta tagaaagttt gacagggcca gttgaacgtt gtgatatttc aacaattaaa
      841 ggtcactgtg aagttttatc agatgaagac agagctttat atatgctatt atcaaaaaat
      901 gtattagaga tagctatgtt aaaaaatatt aatagagatt atagtgaatt agaaaagtat
      961 ttaggagaga gttaggagag aaatagatga aaaatacagc agttacattt aaagaatcta
     1021 aattaagaaa tgaaaaatta acaatgctta cagcttatga ctattcaaca gcaaaaataa
     1081 ttgatgaagc tgggataaat ggaatattag taggagattc tcttggtatg gtatgtctag
     1141 gacatgaaga tacactttca gtaacaatgg aagacatgat tcatcatact agagcagtta
     1201 caagaggagc taagaacact ttagttgttg cagatatgcc atttatgtct tatcaaactt
     1261 cagtatatga ttcagtagtt aatgcaggaa gattaataaa agaaggacga gctcaggttg
     1321 ttaaattaga aggagggata gaagtatgcg ataaaataga ggctatagta aaggcttcta
     1381 tacctgttat ggcgcatata ggtttaacac ctcaatctgt taatgcattt ggaggattta
     1441 aggtacaagg aaaggacaag gaagctgcta aagaattaat aagagctgct aaagctgtag
     1501 aaaaagcagg agcattcgct gtagtgttag aatgtgttcc aactaagctt gcagagttaa
     1561 tttctaaaga gatatcaata cctacaattg gtattggagc aggtgcaggg tgtgatggac
     1621 aaattcttgt atatcaagat atgttgggga tgtatagtga ttttacccct aaatttgtga
     1681 agaaatatgc taatttatca gaagagatga ataaagcatt tacaaaatat atagaggaag
     1741 taaaagatgg agtttttcca ggtccagaac atgggtttaa aataagtgat gatgttttag
     1801 aaaaattata ttaaaagggt caagaaatat ggttatattt aaaacagtga atgatgttag
     1861 aagtcaagta aaagagtgga aaaaacaagg cttaaaggta ggattagttc ctactatggg
     1921 atatttacat gaaggtcatg aaagtttaat tagaaaagca tcaaaagaaa atgataaagt
     1981 tgttgtaagt atctttgtaa atccaactca gtttgggaaa aatgaagatt taggtagtta
     2041 tccaagagac ttagaaagag atatagaagt atgcacaaga ggtagagcaa cagcaatatt
     2101 taatcctgaa gttgaagaga tgtattgtga caatgcaagt acttttgtta atataactgg
     2161 attaacagaa ggtttatgtg gagctagtag gccaatacat tttagagggg tttgcactgt
     2221 tgtaagtaaa ttatttaaca taataccagc agatagagct tattttggag aaaaggatgc
     2281 acaacagtta gctgtaatta aaagaatggt tagggacctt aatattgata tagacgttgt
     2341 agggtgtcca attataaggg aagaggatgg actagctaaa agttctagaa acacatactt
     2401 aagtttagag gaaagatctt cagctactat tttaaataaa tctttaactt tagctaagga
     2461 agctttaaat aatggagaga gagacagctt aaaaataatt gagataataa gtaaaaatat
     2521 aaatacttat aatttggcaa agattgatta tgtagaagtt gttgattctt tatcattaca
     2581 aagagtaaat tatattgaaa aatcagtttt agttgcaata gcagtattta taggaaaaac
     2641 aagattaata gataatttta catttgaatt acaatagggg gctataatga aagttacaat
     2701 gttaaaagga aaaatacata gagctacagt tgaacaagct gagttagatt atgtaggaag
     2761 tattactgtt gatatgaatt tattagaagc agcaggaata tatgaatatg aaaaagtaca
     2821 aatagttgat gttaataatg gaaatagatt tgaaacttat acaatagctg gagagagagg
     2881 aacagggatg atttgcttga atggagctgc agctagatgt gtatcaactg gagataaaat
     2941 aattttaatg gcatattgtg aattaaatga aaatgaggtt aaggatcata gacctaaggt
     3001 tgtttttgtc gatgataata ataaagttga aagagtaaca tcatatgaaa aacatggaag
     3061 gttatcagat ataattcttt aggttttgta atgttattta tatatagcta gactattatt
     3121 gaaatattta ttgattacta attggataga tgtcaataaa gtagacacaa aaagagaaga
     3181 tacagactgt agacaaagtc cattttatgt gaatcggcta cactagacta gacagaaaaa
     3241 ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg
     3301 aagaacctat tcaaaagaat tcaaacaaca aatcgtcaat ctctatctcg ctggtaagcc
     3361 tcgcgcagaa attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa
     3421 gcaagcacaa tcaacgggct cattcaaa
//
LOCUS       DASHWH010000146         3402 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.154, whole genome shotgun sequence.
ACCESSION   DASHWH010000146 DASHWH010000000
VERSION     DASHWH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.154"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(543..1559)
                     /gene="tsaD"
                     /locus_tag="U2R41_002825"
     CDS             complement(543..1559)
                     /gene="tsaD"
                     /locus_tag="U2R41_002825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEM8890156.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1556..2020)
                     /gene="rimI"
                     /locus_tag="U2R41_002826"
     CDS             complement(1556..2020)
                     /gene="rimI"
                     /locus_tag="U2R41_002826"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM8890157.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2027..2569)
                     /gene="rimI"
                     /locus_tag="U2R41_002827"
     CDS             complement(2027..2569)
                     /gene="rimI"
                     /locus_tag="U2R41_002827"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM8890158.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2553..3275)
                     /gene="tsaB"
                     /locus_tag="U2R41_002828"
     CDS             complement(2553..3275)
                     /gene="tsaB"
                     /locus_tag="U2R41_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEM8890159.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          957 a          745 c          591 g         1109 t
ORIGIN      
        1 atttatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactggg caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgataga ttaactagag gaagcgtctt gtcctcatcc gacctagatt
      181 attcgatatt cctcgacaat aaacgattaa tcagtgaggg caatcagagg aacggataat
      241 gatgaataag ctgtaaaaaa tagggctttg acaaaagcgt gtcacagccc ttatttcgat
      301 taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata aacatgagga
      361 gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta cgaactcttg
      421 attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt caaaaacatg
      481 agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagattt ttcacaacct
      541 ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc aaaatttctt
      601 ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc acaaagtcga
      661 agagggggaa tcactagttc tacctcaggg agtttagttg ccaaagcatc ctgcaatcct
      721 tcacgcaacc cttgattggc cgctacacct ccagctacaa tcagttgctt gactggataa
      781 ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc ttgaaagcta
      841 gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg caccaaattg
      901 ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg gatcatcgct
      961 cgtggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc ttttccgctt
     1021 gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc tgcgtcatct
     1081 cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa ttctgtatgt
     1141 cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag acgggctgca
     1201 tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc ccaagcaaaa
     1261 gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc ataagtgaca
     1321 gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc ttccaagcat
     1381 aaggtgattt gttctacgtg atggcggcta gctacttcag gaactacgcc accaaaacgt
     1441 ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc atttctaacg
     1501 accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc ttcattcatt
     1561 tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc ggattgcgat
     1621 aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt tttcttgctg
     1681 cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt tgttccgtaa
     1741 tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa
     1801 tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca cagatctggt
     1861 tatcttcaac taaaagcaga taatcagagt gaggctgttg gatgtctgcc aaaaactgca
     1921 ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat acgcattctg
     1981 ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac tcattttcta
     2041 aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt tttttgatcg
     2101 ttgttgcttg aaaacctaac ttccgataaa gtctttgtgc atcttgatta ctcatacgca
     2161 cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt tcgtcaataa
     2221 gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc gtgatatggc
     2281 aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta tcaaagatac
     2341 ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt tttgtccatg
     2401 gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata tcttctgtag
     2461 tcaattctct tagtacgtaa cgacgatcga tttgttcgat tgttttttct gtataaatca
     2521 ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata actttcgtct
     2581 ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta cccgttttaa atagcgagga
     2641 agaaaagctt gtttctctgc aactggttca gctaaagcac ctaattgagc cacagttaca
     2701 ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa tatttccttt
     2761 atttcataga agaatttgtg acaatcactc ccgacgaaat aaatagcttc attcagcttt
     2821 tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac aatttctaat
     2881 ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc atcaaacatg
     2941 ggcacaataa ttttatccac accgatgcaa ttagccgcga tggttttcag actagatact
     3001 cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac acctatacga
     3061 agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc ttttggactt
     3121 atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact gtggttgcgt
     3181 ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa
     3241 gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc tgctatgaca
     3301 ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc aaaggcaaaa
     3361 ctagtacaat gatacatgat tatctgctgg cgggcaaatt tt
//
LOCUS       DASHWH010000147         3394 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.155, whole genome shotgun sequence.
ACCESSION   DASHWH010000147 DASHWH010000000
VERSION     DASHWH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.155"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..272
                     /locus_tag="U2R41_002829"
     CDS             <1..272
                     /locus_tag="U2R41_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEM8890160.1"
                     /translation="IKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINEL
                     IEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            415..636
                     /locus_tag="U2R41_002830"
     CDS             415..636
                     /locus_tag="U2R41_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8890161.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            652..1494
                     /locus_tag="U2R41_002831"
     CDS             652..1494
                     /locus_tag="U2R41_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890162.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1557..3359
                     /locus_tag="U2R41_002832"
     CDS             1557..3359
                     /locus_tag="U2R41_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEM8890163.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1226 a          468 c          702 g          998 t
ORIGIN      
        1 tcatcaagct ggctaataag ggtcaagatt atgaacatct agcagatgag attgatgagc
       61 tgagagacaa gcgacagctc cttttagtag aagatgcctc cctcagtggc gagaacgagc
      121 gaatcaatga gctgattgaa tttatccgca agaacaaatt ccgtacctta gagtacgatg
      181 ataagcttgt aaggaagata atccagagcg ttacagtcta tgaagaacac ttcgtcatat
      241 ccttcaaatc tggcatcgaa atggaaatat gaaaaccaga agtaaatagc ccatgattct
      301 acagtagagt cgtgggtttt cttttgcttg ctgattataa taatatttta ataactattg
      361 ttattataaa ccaaatgatt tataatattc ttagcgtaaa cttggaggtg ctgtatgact
      421 acggcagaaa tgattaaaga actgtgtgag caaatgaata taagtgtttc cgaacttgct
      481 agacgtattg gacagactcc acagaatttc aataagaaat tacaacgaga aacggtaacc
      541 ttagatgagt tgaaagcgat tgctgatgtg ctaggtgtca agtttgtgca ggcttttata
      601 ttgcctgatg gcaatgaaat aaagataggt aattaggagg tgtaatttgt attgaaaatc
      661 ataacaataa atgatgttga gtatgctgta tttgctgcta atgaaggtac ttccaaacca
      721 caacctcata tcatagaaac gaaatctggt actattccag aaggaaaaca actgagttta
      781 ttaaaagaat atcttaagca gaatgatatt tccccaataa aaggagcaac aacatattgg
      841 tgcattgaca aggttcttaa gcttgattca agtaaagaaa aaactataag tgaaacaatc
      901 cataaacaaa aatacttatc tttaactgaa gaaaatattg agaaacagca taagtttgta
      961 ggtgcaagct ctaattatgg gaaagaaggt ctaataatac atgatgtttt gaatgcattt
     1021 cctcttcata atgatttaaa tactattgcg atgaagattg cggtaattga tgtaacaaat
     1081 tcaacacatc tttctcaata taagagcagg ttgtctctat atgatttggc taaagtaatt
     1141 ctggagattc caaattttga tgatcggttg gcaaaaggag atcctcagtt gataaatatt
     1201 atcgcaagga acattggtgc agttaatatg ttctcatttg catccaagta ctgtacatat
     1261 cataatgtgg aagtgtgtgg gagagatgat tactccatct ttgatggtat cgttaaaaat
     1321 actcttccac actatatcca aggactaact acgaataaga ttgatacgtg gcgaagaagt
     1381 tttgattacg aggcatttaa cgaatgtgtt ggaaaactac ttgatgaaaa taatatacat
     1441 attcctttta gaagaagaaa gttagatcac ttcttgtggt atgcgaatag gtagaatata
     1501 agaaaaaggc atcgtaaaag tgattctgga taaaattaat gggggaggaa ataatagtgg
     1561 gattatttga ttttctaaag aaaaaacaag gaaaggttag taaacacgtt gattatcaac
     1621 tggcttctag tctatttcgt actggtaaag tttttgctga aaaattggaa gtggacatat
     1681 caaatgtaga tgagtttcgt aaaactttta tcgcttttga tgtggaaaca actggcctaa
     1741 attcttcttc agatagaatt gtagagattg gtgcggtgct atttaaagaa ggagttccaa
     1801 ttcaatcatt cggaactctt gtcaatccaa aagttagaat tgcagcctca gcttcggcaa
     1861 taaataatat aactaatgaa atgctaaact ctgctccaaa tgagcaggaa gtttaccccc
     1921 agttggtaga atttttagga gatgcactag atggaaaaat cactatgtgt gcacacaatg
     1981 caagtttcga ttttggattt ttaagtaaca cattttctag acttgggtat gatgctaaaa
     2041 ttaagtatgt tgatactctg agtctctcta gaaaacatat aaaaggcatt gtaaatcata
     2101 agcaagagac tgtagcggaa tatttcggat taataaatga cactgcccat agagcggaat
     2161 cagatgcaga tatatgcgga aagatttttt ggggtgtttt agatattatt gaggagtcgt
     2221 tggaagaaca aaaaaagcaa atagaaaaga tgactccaga cataaaagaa cttgaagctt
     2281 gtgcttttat tcaagatatt attgagaaaa aaggatgcga caccagttgg atcaggtatc
     2341 gaaaaaacag taataattat gtcgatgtaa cttgtctata tacattctta aagtttaagt
     2401 ttgctaagaa gggaaattac atcattatta gaaaagatgc tacaaatgga attgatttac
     2461 ctatagaacc ttgtactgta agtgaaggcg gtacaacaaa tgtgcgagtc tattttcgta
     2521 gcccctttga cttaacgcct ctttcaaatt atattttcag tgcttatagc gattgttata
     2581 attctatgaa tgaatatatg agtgttagta attacactaa aaaagaagca gagcaaagcc
     2641 ttagtatgct gaaatctatt tcaaaatcag cgatgaaaga gttattattc gatgcaaaaa
     2701 atcgtggata tgatgagact gaagttaacc taaatattga aaaagtgata tctagagaag
     2761 atgtaattat taacgggttg cataatcgag tttcattaaa caagatacga aatataggga
     2821 attgggagaa aggatttgat gcaggttatc catattggga acgtggagaa actgctagga
     2881 aaaatggcaa agtagatgaa gctattgcac tttttgataa agctcgatat aacggctatg
     2941 atgctcctgc gctatatgat tcttatgcta aagcttatcg aaaaattaag gattacgata
     3001 atgaaattct cattcttgat gaaggaatta tgcgtaaaac acggcacgat gttggtactt
     3061 tagaggctcg tagggataag gcaattaagc tattatttgc taaacaagag gcagagcgga
     3121 tagctaagga aaaatctgat tttctaaaag cgaacaaaaa agaggatgcg attagtgagc
     3181 ctagcaagaa gcgtggaaga cagattattc aaatgactga tgatggaaca ataataaaag
     3241 aattcgatac tatagcttct gctgtaaaag aagttggcgt taactcaaaa agtattaggg
     3301 atgctgcaaa tggagttcaa aaacatgctg gaggatactg ctggaaatac aaagattagc
     3361 agataatcta agataaccta ggatcttctt aata
//
LOCUS       DASHWH010000148         3374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.156, whole genome shotgun sequence.
ACCESSION   DASHWH010000148 DASHWH010000000
VERSION     DASHWH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.156"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U2R41_002833"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U2R41_002833"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEM8890164.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U2R41_002834"
     CDS             complement(2261..2512)
                     /locus_tag="U2R41_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEM8890165.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U2R41_002835"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U2R41_002835"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEM8890166.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1088 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttt
//
LOCUS       DASHWH010000149         3293 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.157, whole genome shotgun sequence.
ACCESSION   DASHWH010000149 DASHWH010000000
VERSION     DASHWH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.157"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            38..589
                     /locus_tag="U2R41_002836"
     CDS             38..589
                     /locus_tag="U2R41_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HEM8890167.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1519
                     /locus_tag="U2R41_002837"
                     /pseudo
     CDS             1265..1519
                     /locus_tag="U2R41_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1589..2101
                     /locus_tag="U2R41_002838"
     CDS             1589..2101
                     /locus_tag="U2R41_002838"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890168.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="U2R41_002839"
     CDS             2098..3135
                     /locus_tag="U2R41_002839"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8890169.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          654 g          871 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa cac
//
LOCUS       DASHWH010000150         3152 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.158, whole genome shotgun sequence.
ACCESSION   DASHWH010000150 DASHWH010000000
VERSION     DASHWH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.158"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(61..2424)
                     /locus_tag="U2R41_002840"
     CDS             complement(61..2424)
                     /locus_tag="U2R41_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEM8890170.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKTGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="U2R41_002841"
     CDS             2872..3093
                     /locus_tag="U2R41_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8890171.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          852 a          654 c          570 g         1076 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctgttttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa ca
//
LOCUS       DASHWH010000151         3008 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.159, whole genome shotgun sequence.
ACCESSION   DASHWH010000151 DASHWH010000000
VERSION     DASHWH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.159"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            441..725
                     /locus_tag="U2R41_002842"
     CDS             441..725
                     /locus_tag="U2R41_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890172.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            728..1144
                     /locus_tag="U2R41_002843"
     CDS             728..1144
                     /locus_tag="U2R41_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890173.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1281..2000)
                     /locus_tag="U2R41_002844"
     CDS             complement(1281..2000)
                     /locus_tag="U2R41_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM8890174.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2653..2748
                     /locus_tag="U2R41_002845"
     CDS             2653..2748
                     /locus_tag="U2R41_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEM8890175.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT         1008 a          565 c          478 g          957 t
ORIGIN      
        1 aaaaaaactc ttagaagcga ttttagagcc atttaaaccc acttaattat attctccatg
       61 aaattcgctc catgcgtcaa aagctgcgtc tgaaattccg ccgtcttcgt tcactagttg
      121 cattaatttt tccaacgatc gttttctttc gggtgtcata tttttatatt ccaacaattt
      181 ttcgtctgtt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
      241 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
      301 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
      361 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
      421 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
      481 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
      541 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
      601 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
      661 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
      721 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
      781 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
      841 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
      901 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
      961 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     1021 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     1081 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     1141 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     1201 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     1261 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     1321 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     1381 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     1441 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     1501 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     1561 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     1621 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     1681 ttttttgttt tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca
     1741 tcttcagtac ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca
     1801 aaggcttttg tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat
     1861 ctaacttttt tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc
     1921 tgcttagcat tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt
     1981 aattccgttt tttcatccat tcctttcttc cccccttaca aataaataat aacctttaac
     2041 aaccaactcg taaataggtt ggttgttatt atacaaaagg atattagtac tcagataaaa
     2101 agttgttagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
     2161 ataaaaagtt gttagtaccc ttctgtatcc gttgggagag taaggctcaa ctgcccccta
     2221 aaaagggttc taaaaaatct ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa
     2281 tttcccgcca aaaaatcacc cactttttaa aataaacagg ttcgcgcatg atattaaact
     2341 gccaatctag ctccctatgg tcgctgtaag aaggagtgat cgctacgctc tcacatttta
     2401 cttgcagcag tcgcctttag gctccttcgc caaaaataat tcattaggga ctcctttgtg
     2461 gcgtcctgag agctcgtcta agccgttcta agacaagatt cgataaaaac tatgtccttc
     2521 gttctaaagc gccttacagg cggtcagggc aagccctaac aaccccaagg caataaaaaa
     2581 cttaggctta tttttgcttt tttattgtat aattgattgt cgcctatccg acagtcttgc
     2641 gactggaagg aggtgtgttt cgtgttcact aactttattt gctcccttct tgttgggctt
     2701 ttaagcgctt tgttcagtta ctggttgaat aaccgaaaca agaagtaaaa cttaattaag
     2761 gcgactcagc ccacaaaaaa agccccagag aatccgacct ctctggggat ttcgtgtgtt
     2821 tcgtgttcac ttatttgcaa tcaaattata acagaaataa atatttattt caaaaatatc
     2881 ctacaaaagt ctaaaatccg taattatttc ttatatgcaa catttacaaa taaagtcgct
     2941 ctgagagctc gtatttttcg atttaagacg ttttatttag tttttggtgt atttcattgg
     3001 tccaagct
//
LOCUS       DASHWH010000152         2917 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.161, whole genome shotgun sequence.
ACCESSION   DASHWH010000152 DASHWH010000000
VERSION     DASHWH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.161"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            204..1172
                     /locus_tag="U2R41_002846"
     CDS             204..1172
                     /locus_tag="U2R41_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM8890176.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="U2R41_002847"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="U2R41_002847"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM8890177.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="U2R41_002848"
     CDS             2202..2810
                     /locus_tag="U2R41_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM8890178.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaa
//
LOCUS       DASHWH010000153         2871 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.162, whole genome shotgun sequence.
ACCESSION   DASHWH010000153 DASHWH010000000
VERSION     DASHWH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.162"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..152)
                     /locus_tag="U2R41_002849"
     CDS             complement(<1..152)
                     /locus_tag="U2R41_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8890179.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(226..507)
                     /locus_tag="U2R41_002850"
                     /pseudo
     CDS             complement(226..507)
                     /locus_tag="U2R41_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            969..2351
                     /locus_tag="U2R41_002851"
                     /pseudo
     CDS             969..2351
                     /locus_tag="U2R41_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2587..>2871)
                     /locus_tag="U2R41_002852"
     CDS             complement(2587..>2871)
                     /locus_tag="U2R41_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8890180.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1010 a          519 c          483 g          859 t
ORIGIN      
        1 acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa caattcctga
       61 acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct
      121 tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc ttttttctca
      181 attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccgaaat gagtatagta
      241 tgaattagta atttggtttt catctaagtt tagctttgat ttactaaaat tgatatctcg
      301 atacatgttc aatacatcta atacaaatcg agaatcttct ttcggaaatg gctcagaaag
      361 atcactccaa atttcatcat cataatgata ctcgtaacct tctctcaata tttcgatttt
      421 tctttcataa taagatctgt tttggtcctc aaatttagcc aataactcaa attgatttac
      481 tagtgacaat ctctgaaaac tgtccattaa atgtttcttc ctatcttgtt cattaacaaa
      541 aaattataag cccttacaac gaatattata tattaaattt aattgtaggt gaacacgaat
      601 tttataaata tttatggtgt taatgacaaa tgatatccga ctcctcttca tacgttctgg
      661 actatttgct aatagattaa aatttcagca tctatttctg accataaata aatgagctgc
      721 cgaaaagctg ccaaatcaga cttgaaaata aaggatcaaa aacttttaag tttctgaccc
      781 cgtaactcac acttcaaaat cttacttaaa aaggagtctg aaagaaaaaa agagtattca
      841 aaattttttt ctaactagct acatcacatc tatgcattga cactcaaaga accagaataa
      901 gtcatcagaa agcaaggggg tgcggacata atcctggacg aaaaaataaa cttaggagga
      961 atgtccgtgt gttttcccac aaagagcgtg taagggcaat tcagttattt ttaaaatacg
     1021 attgctctta tgctgcaacc attcgagaat tagggtatcc gtcgattggt gcactacgga
     1081 aatggtataa agagtatttg gaatccggag agctccatca ggagcaccga aaaaaatcaa
     1141 agtattctga agaacaaaaa cgaatagcgg tgaatcatta ttttgaatat ggtcaatgtt
     1201 atgcacgaac cattcgcatg cttggttatc ctaatagaga gtccttgcgg cagcggtgtg
     1261 aagaacttgc accaggagct agaaaactcc gaaaaagtgc ggtaaagtta acacaagatc
     1321 aaaaagaagc tgtgttgaaa aaattctata aaccgcaaac taataggaaa aatttagccg
     1381 aatctggagg aattagtaga gttactctat atcaatggaa aaacacggtt cttggaaagg
     1441 attttccatt gtgtatgact ttaaaaaatc aggaaaaaca aaaagagcta cttttaaagg
     1501 aagtagaaga cttacaaaaa caagtacacc aattacagct cgaaaaagca ttactcgaag
     1561 gagcggctga actgctaaaa aaagaaaaag gcgtcaatct tctttgtcta tcaaatcaag
     1621 aaaaaacgat attgattgat gccttacgaa atcagttcac cttgaaagag cttcttcaac
     1681 agctccagtt tcctaaaagc agctattttt atcaaaaaca agcacttgag aaaccagata
     1741 agtattacaa agaacgtcaa attaattatc acgattttca atcataactt ctgtgcgtat
     1801 ggttaccgta gaattcacca agctttaaaa aatatgggta aaaagctttc tgaaaaagtt
     1861 gtacgtagat tgatgactga ggaaaatcta tttgttaaat tttctagacg aaaaaagtat
     1921 tcttcgtatg ctggggaaat ttctcctgca caccctaatt tattaaatcg gaattttaaa
     1981 gcaaagaagc caaatgaaaa atggttaaca gatattactg aatttagaat tctcgcaggg
     2041 aaaatctact tatcaccact tgtcgattgt tacgatggtg ctattgttag ttggactatt
     2101 ggaacaaagc caacttgctt cactctatgt ctaaaaaggg ttattcacca gataattcag
     2161 cgtgcgaagg tttctttgga cgattaaaaa acgagatgtt ttatcaacgt gactggaaga
     2221 atacaacaat caatcagttt ataaacaaat tggatgatta tattcattgg tataataatc
     2281 aacgaattaa gttatcttta ggagggctaa gccctcttca atatcggaag aagcaaagat
     2341 atatttgtta aaaaggtcca aaaaaaatat ccgcaccccc ttttttttgt aaaaaactat
     2401 tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc aagaacttat
     2461 ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc tgcctaactg
     2521 gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg aagagccttt
     2581 tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga gcgttccagc
     2641 gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt cgacataccc
     2701 attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt gtttcatcac
     2761 accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt aggcacattg
     2821 agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct c
//
LOCUS       DASHWH010000154         2778 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.163, whole genome shotgun sequence.
ACCESSION   DASHWH010000154 DASHWH010000000
VERSION     DASHWH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.163"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..197
                     /locus_tag="U2R41_002853"
     CDS             <1..197
                     /locus_tag="U2R41_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890181.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="U2R41_002854"
     CDS             314..811
                     /locus_tag="U2R41_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8890182.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="U2R41_002855"
     CDS             898..1290
                     /locus_tag="U2R41_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8890183.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="U2R41_002856"
     CDS             1274..>2778
                     /locus_tag="U2R41_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890184.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DASHWH010000155         2775 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.164, whole genome shotgun sequence.
ACCESSION   DASHWH010000155 DASHWH010000000
VERSION     DASHWH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.164"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..129
                     /locus_tag="U2R41_002857"
     CDS             <1..129
                     /locus_tag="U2R41_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8890185.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..678
                     /locus_tag="U2R41_002858"
     CDS             130..678
                     /locus_tag="U2R41_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890186.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            938..1666
                     /locus_tag="U2R41_002859"
     CDS             938..1666
                     /locus_tag="U2R41_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEM8890187.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1696..>2775
                     /locus_tag="U2R41_002860"
     CDS             1696..>2775
                     /locus_tag="U2R41_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEM8890188.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1010 a          371 c          593 g          801 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tccaattgtt ccagaattag atgatcgtgg agaaataata
      601 agaactacca gtcagataaa acaagatacc gaagactcac tgcgcaaagg gtggggaatg
      661 aataataaag ttagataata atcaaagaag aactaagtaa ctacctttaa acggtagtta
      721 cttagttctt ctttgatttg cgtgaaatag aggggattta tttttctggt tacgtgatta
      781 aaaaatccaa aaaatgagca taggaacggt tatgatttta gggtgtgttt taatgagata
      841 ttgtgatttc attaacaagt gtatgataat ctgttgttga gttagtaaat aagtaataga
      901 gctaactcta aaataaaact attaggagga gatgtaggtg aaaaagttta aaaagaaaat
      961 atatttagga ttggttgttg gggtgagtat gtttgggttc atggctcata ctaaacaagt
     1021 aactgcagat actaaccaaa aagaaaggat tatgagccaa ttagatgact ctcaaataga
     1081 gtatgtaccc aatgagctaa ttgtcagttt aaaggctaca caaagaattt caacagagaa
     1141 aaataaaccg caaaatttat tgggtgtaaa agttatggtg gaaaaagatt tgactcttag
     1201 gacaaatgaa agcgattata ataagcgaga tcaagtatta ttagtgaaat tgaacgaaga
     1261 aattagtgtg gaagaggcag taagaacatt taatgcttct gatatggtca attatgcaga
     1321 gccaaactat gtggttgaac tagagccgtt gaatgatgtt gaggagccaa atacaactga
     1381 ttctttagga aatcgatcaa ttattcttaa cgatcctaat tggtgtaaac aagctaattt
     1441 aagagagatt agcatgccat ccgcttggga agaaaccaag ggttctaagg atgtgaaggt
     1501 agcggtcatt gattcaggaa ttgattatca acatgaagat ctttctggaa atgtagatga
     1561 aagtttggga tacgattttt ttgacgatga tacagatcct atggatacta tgggacatgg
     1621 acacatattg ccgggattat tggtgctcaa acaaacaacg aattaggtgt tgctggaata
     1681 aattgggatg ttacgatggt acctatacgt gtatttggta gtagtggagg taccagtttg
     1741 gcaatcttaa ctgaagctat taattatgca tcccaaaaag ggtttccaat tatcaatttt
     1801 agtataggta gtgttgttta tagcagtaca gaaaaagcaa ttaaaaacta tcctggatta
     1861 ttcgtagcag cagctggaaa taatgggata gatatgactg ataaaccttt ttacccagct
     1921 tctttggata tacctaatat tattactgta ggaaattcta gtggttatga acgaagttct
     1981 gattcaaatt ataggaaaac aggtgttgat ttattcgcta gagggtttcg tgtttttagt
     2041 acatttctag gaagtaaata cacgtatatg tcaggtacct cgatggcaac acctcatgtc
     2101 actggtagtg ctgcattagc gttgtcacta gatcctgatc tatctactga tgaattaaaa
     2161 aaaatcattt taaatagtgt tgattcattt tatgaactat ctgatttatg tcttacaggg
     2221 ggaagactaa atacgaataa tgtcgtacga caagtaacgg gtaaacttac tgcatctaat
     2281 atcacattaa gattaaatag tgaatggtca gatgaaattg ctaaagagat gtgtcacgtt
     2341 cactggattg accagtcagg aaatgatttg acggatcaag tggttgttct aaaaaacgaa
     2401 gtggatacat ctaactttgg cgattacgaa gtagtttttg catcacctga tttaacagat
     2461 caaatctcag ttacagtgac cgtacctcga ttacgtaaac ttacgctagg aaaagaaaca
     2521 gcaaccattg atattggttc cgcatgggat agtgtcatag ccattgatac ttttcaagtg
     2581 aaagctatag atgattttgg gaatgatgtt acaaatcaag tacaggttac tattaatgct
     2641 gatatagata caattaattc aattcaagca gtgattaagt ttgaagttcc agaattaggc
     2701 ttagcccaaa ttggtagact gaatattact gcaaaaaata gtacaatcaa tggattgaat
     2761 gaagttagca tcgag
//
LOCUS       DASHWH010000156         2769 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.165, whole genome shotgun sequence.
ACCESSION   DASHWH010000156 DASHWH010000000
VERSION     DASHWH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.165"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            75..692
                     /locus_tag="U2R41_002861"
     CDS             75..692
                     /locus_tag="U2R41_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890189.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            696..1145
                     /locus_tag="U2R41_002862"
     CDS             696..1145
                     /locus_tag="U2R41_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890190.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            1145..1639
                     /locus_tag="U2R41_002863"
     CDS             1145..1639
                     /locus_tag="U2R41_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890191.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1653..2084
                     /locus_tag="U2R41_002864"
     CDS             1653..2084
                     /locus_tag="U2R41_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890192.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2086..2223
                     /locus_tag="U2R41_002865"
     CDS             2086..2223
                     /locus_tag="U2R41_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM8890193.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            2260..2553
                     /locus_tag="U2R41_002866"
     CDS             2260..2553
                     /locus_tag="U2R41_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890194.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2550..>2769
                     /locus_tag="U2R41_002867"
     CDS             2550..>2769
                     /locus_tag="U2R41_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8890195.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT         1007 a          403 c          603 g          756 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
      661 attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta aaaataaaac
      721 aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
      781 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
      841 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
      901 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
      961 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1021 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1081 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     1141 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     1201 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     1261 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     1321 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     1381 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     1441 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     1501 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     1561 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     1621 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     1681 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     1741 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     1801 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     1861 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     1921 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     1981 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2041 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     2101 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     2161 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     2221 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     2281 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     2341 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     2401 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     2461 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     2521 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     2581 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     2641 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     2701 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     2761 gaggaaaca
//
LOCUS       DASHWH010000157         2767 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.166, whole genome shotgun sequence.
ACCESSION   DASHWH010000157 DASHWH010000000
VERSION     DASHWH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.166"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            179..775
                     /locus_tag="U2R41_002868"
     CDS             179..775
                     /locus_tag="U2R41_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8890196.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="U2R41_002869"
     CDS             899..2698
                     /locus_tag="U2R41_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM8890197.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          790 a          480 c          522 g          975 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgc
//
LOCUS       DASHWH010000158         2719 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.167, whole genome shotgun sequence.
ACCESSION   DASHWH010000158 DASHWH010000000
VERSION     DASHWH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.167"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="U2R41_002870"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="U2R41_002870"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8890198.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DASHWH010000159         2652 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.168, whole genome shotgun sequence.
ACCESSION   DASHWH010000159 DASHWH010000000
VERSION     DASHWH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.168"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>2652)
                     /locus_tag="U2R41_002871"
     rRNA            complement(<1..>2652)
                     /locus_tag="U2R41_002871"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          551 a          802 c          557 g          742 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccagcagt cctcacgatc tgccttcaac
     1561 gcccttagaa cgctctccta ccaatacacc taaaggtgta ctccacagct tcggtaatat
     1621 gtttagcccc ggtacatttt cggcgcaggg tcactcgact agtgagctat tacgcactct
     1681 ttaaatggtg gctgcttcta agccaacatc ctagttgtct gtgcaacccc acatcctttt
     1741 ccacttaaca tatattttgg gaccttagct ggtggtctgg gctgtttccc tttcgactat
     1801 ggatcttatc actcacagtc tgactcccgg atatgaatga atggcattcg gagtttatct
     1861 gaattcggta acccgagatg ggcccctagt ccaaacagtg ctctacctcc atcattctca
     1921 attccgaggc tagccctaaa gctatttcgg agagaaccag ctatctccaa gttcgtttgg
     1981 aatttctccg ctacccacac ctcatccccg cacttttcaa cgtacgtggg ttcggtcctc
     2041 cagtgcgttt taccgcacct tcaacctgga catgggtaga tcacatggtt tcgggtctac
     2101 gactacatac tcaaacgccc tattcagact cgctttcgct gcggctccgt ctcttcaact
     2161 taacctcgca tgcaatcgta actcgccggt tcattctaca aaaggcacgc catcacccat
     2221 taacgggctt tgacttgttg taggcacacg gtttcaggtt ctatttcact ccccttccgg
     2281 ggtgcttttc acctttccct cacggtactg gttcactatc ggtcactagg gagtatttag
     2341 ccttgggaga tggtcctccc ggattccgac ggaatttctc gtgttccgcc gtactcagga
     2401 tcctcctagg tgtcttccac atttcgtcta cggggttttt accctctttg actgactttt
     2461 ccaagtcatt cgactatctg aaagaactac catattggag tcctacaacc ccaacaagca
     2521 agcttgctgg tttgggcttt tcccgtttcg ctcgccgcta ctcagggaat cgaattttct
     2581 ttctcttcct gcaggtactt agatgtttca gttctctgcg tctacctcga atgtgctatg
     2641 tattcacaca at
//
LOCUS       DASHWH010000160         2648 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.169, whole genome shotgun sequence.
ACCESSION   DASHWH010000160 DASHWH010000000
VERSION     DASHWH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.169"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            704..2563
                     /gene="ltrA"
                     /locus_tag="U2R41_002872"
     CDS             704..2563
                     /gene="ltrA"
                     /locus_tag="U2R41_002872"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8890199.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1025 a          421 c          507 g          695 t
ORIGIN      
        1 aggcacttaa aagaagaaaa cataagtttc cttttaagga gaactgatat tttgagcagt
       61 ggaatgatgg ggtaacgccc tgaaacgctc ccttaatact ccgactggct gtttaacata
      121 tagctgttag atggtcagaa gctcggtgaa gtcggctgaa agttaccgta aatatgataa
      181 attcatatac gaaagttgtt cagaggtgaa tgatgtaacc tataggtcgg aggtctataa
      241 aatactcacg gtgagaatgt aagtaatact actgacgaac ttgcgaatga aagaatctat
      301 acacggactg tatagaaatg tacaggattc taccgtatca acggaacaga atgtatgttg
      361 tggtttagta agattattgg ttatgaacaa ccatgcaatg ttacaggcat tggaaatata
      421 caggttcaaa gcaagcacct aaaagtatat ataaagataa aaatatcgaa acatgggaag
      481 ctggcaatgt ggaggaaact atattccatt gaaacatttg tgacgaagaa taataagcac
      541 atttatgcca gtgaaagtgg tggcacagta ccaatgagga tatagaaata tatcggagga
      601 atagccacta gtcaaaagaa atcgttaatt aaatgatgta tttcatagaa tcgagtaaga
      661 caaaataaac actacaacct aagtaaacaa aggtagtggt gctatgaata aagtaaaaac
      721 aaataaaaca cttcgtcata gtgaatacta tggcatgaca caaatatttg atgaactgta
      781 tgtaaaaagt gataaaaaag aagtttttac agagctcatg acaattatta tgagtgatga
      841 aaatattcga ctagcataca gaaatatcaa gaataacaag ggcagttata cagcgagtac
      901 cgataagaaa agtattcgag acattaaaac actgtcagaa gatacctttc taactgaggt
      961 taaagttctt tttcgctgtt acaaaccacg agcagttaga cgtgtagaaa ttcccaaacc
     1021 aaatggaaaa acacgccctc tgggcattcc atcaatctgg gataggttaa tacaacagtg
     1081 tattttacaa gtcttagaac cgatatgcga agctaaattt cacaattata gttatggatt
     1141 tcgaccaaac cgttccacac atcatgcgat tgctacctgc aatcgactgg caaatagggg
     1201 gaagctacac tttgtagtag atgttgatat taaaggattc tttgataatg tcaatcactc
     1261 taaacttttg cgtcaaatgt ggacgattgg aatccgtgat aaatggctat taggtattat
     1321 tcgatccatg ctaaatgctc ctatagtcct accaaccggt aaagtcgaac accctaaaaa
     1381 aggtgtacct caaggtggtg tcctatcccc tttactatct aatatcatat tgaatgaatt
     1441 agattggtgg gttgcttcac aatggcaaaa taccacgatt gagacagaaa agattatgga
     1501 taggcgagat aaaggcaaag gcattgataa gactaatcag tatcgggcat taagacgaac
     1561 aaacttaaaa gaaatataca ttgttcggta tgctgacgac ttcaaaatct tttgtagaaa
     1621 acgaagtcat gcagataaaa tatttcacgc aacgaaaatg tggcttaaag aacggttatc
     1681 acttgaaatt agcgaagaaa aatccaaaat tgtcaatctc aaaaaaggta aatcagaata
     1741 tctaggattt gaattaaagc taatcaagaa aggtaaaaaa tacgtagtcg aaagtcatat
     1801 gagcaaaaaa tcaatgacaa gagttaaaat ccaattaaag aaacaattac gtaaagttgc
     1861 cagaccgaag aatcattgcg aacaagcaaa agaaattgga ttatataatt ctatggtgat
     1921 tggaatccat caatattatg gaattgcaac ctgtgtaaat ctcgattgca gtaaaatagc
     1981 ctatacacta aaaccgttca tcaatcacaa actgccgaca aagaaacatg gaaagatatt
     2041 aaatacattt atcaaatcga agtatggaaa aagtaaagaa atgcgatggt tgaataatgt
     2101 gccaattgta ccgctcaact atgttcagtt tagacttgct gtccccctat cagagggaac
     2161 ttgcaagtac actgaaagtg gacgaagcaa gatacacagt aaattaaaat tagacctagc
     2221 ccttttactt cacatgatga gaaatagtaa ttatgaaaga agtattgaat ttgtggataa
     2281 cagaatatct aaatattctg ctcaaaaggg aaaatgtgct atcacgggta aatttctcga
     2341 atacgaagaa atccattgcc accatatcgt accagtaaaa caacagggta cagacaaata
     2401 cagcaacctc attattattc acaaaaatgt tcacgcatta gtccatgcta tcgaagaaaa
     2461 aatcatacac aaatacctga atgtattaaa tctaaccaat gaacaaatag aaaaactaaa
     2521 tgaattacgt gtcaaagctg gtaatagtgt tttaacagtt taacttgaaa aatatatcat
     2581 acttaacatt cttttgatgg aacgccgtat gaagtgaaag tttcatgtac ggtgtgaagc
     2641 gggggaaa
//
LOCUS       DASHWH010000161         2624 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.170, whole genome shotgun sequence.
ACCESSION   DASHWH010000161 DASHWH010000000
VERSION     DASHWH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.170"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="U2R41_002873"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="U2R41_002873"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8890200.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          942 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaa
//
LOCUS       DASHWH010000162         2603 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.171, whole genome shotgun sequence.
ACCESSION   DASHWH010000162 DASHWH010000000
VERSION     DASHWH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.171"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..177
                     /locus_tag="U2R41_002874"
     CDS             16..177
                     /locus_tag="U2R41_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890201.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            190..693
                     /locus_tag="U2R41_002875"
     CDS             190..693
                     /locus_tag="U2R41_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8890202.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="U2R41_002876"
     CDS             753..1142
                     /locus_tag="U2R41_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8890203.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..>2603
                     /locus_tag="U2R41_002877"
     CDS             1129..>2603
                     /locus_tag="U2R41_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890204.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          773 a          430 c          600 g          800 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg gttcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tgg
//
LOCUS       DASHWH010000163         2546 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.173, whole genome shotgun sequence.
ACCESSION   DASHWH010000163 DASHWH010000000
VERSION     DASHWH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.173"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            386..1966
                     /locus_tag="U2R41_002878"
     CDS             386..1966
                     /locus_tag="U2R41_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEM8890205.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2164..2388
                     /locus_tag="U2R41_002879"
     CDS             2164..2388
                     /locus_tag="U2R41_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEM8890206.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          904 a          414 c          534 g          694 t
ORIGIN      
        1 agatttctct ttaccaacat catttgacaa agagcccttt tcttttagtc agctgacaaa
       61 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
      121 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
      181 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
      241 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
      301 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
      361 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
      421 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
      481 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
      541 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
      601 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
      661 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
      721 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
      781 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
      841 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
      901 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
      961 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     1021 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     1081 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     1141 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     1201 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     1261 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     1321 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     1381 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     1441 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     1501 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     1561 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     1621 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     1681 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     1741 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     1801 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     1861 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     1921 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     1981 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     2041 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     2101 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     2161 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaaaga agagatgtta
     2221 cgattatgtc gattattaga tgtaaaagac tgggacaaaa acgagcaaat tgcacaaaaa
     2281 gtacgatcaa tcatccataa agaggaaaaa gttaaaaata aagacacgcg tagaactgaa
     2341 aatgaaaaga gaaaagtaca aaagaaaaga gaaaaatcaa aaagctagaa agaaaaagag
     2401 gctgagacag aagcgtttaa ctccaagaaa taagctggaa ttcacaaaaa tttgaaaagc
     2461 aattttcgtg aatttcagct tatttccgaa ggagttgctt ctgctttcgc cgtttatacg
     2521 tgtttagagc gtgagacaaa agtgtt
//
LOCUS       DASHWH010000164         2483 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.174, whole genome shotgun sequence.
ACCESSION   DASHWH010000164 DASHWH010000000
VERSION     DASHWH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.174"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1014..1304)
                     /locus_tag="U2R41_002880"
     CDS             complement(1014..1304)
                     /locus_tag="U2R41_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890207.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="U2R41_002881"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="U2R41_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DASHWH010000165         2467 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.175, whole genome shotgun sequence.
ACCESSION   DASHWH010000165 DASHWH010000000
VERSION     DASHWH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.175"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(492..1784)
                     /locus_tag="U2R41_002882"
     CDS             complement(492..1784)
                     /locus_tag="U2R41_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8890208.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2058..2318)
                     /locus_tag="U2R41_002883"
     CDS             complement(2058..2318)
                     /locus_tag="U2R41_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890209.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          813 t
ORIGIN      
        1 tatattataa attcacatga ttatcgaatt ttattaagtt agtttttctt gtaaatctct
       61 gttcccaatg aaactcgaga cacttatttt aaagaggaaa caatgtggta tttaaatagc
      121 cctcttgtat gggctactct gtgatactta ttagccatta aactatttta cccacatagc
      181 atatattttg aaatcgttca ttccgtaaat tcaactaaat atgcctaaaa aaataaaaaa
      241 tctaagatag aataagattt attcagtctt agatggattt atagcaactt tttcgtaaca
      301 actttaaaaa atacgaaaag aaagagctgt tttactctga ctacgttgct aaattgtcgt
      361 ttaatcattc atttaaacca aatctttttt tatagaattc gttttaatcc tcctgaatcc
      421 aaaaactatg aaaaccgtaa ttaaatagtt aagagttttt aaccaccata attctctcgg
      481 ctattttgaa atcagataaa ttttgatcgg acacatagtt gaataaacct accgttgtta
      541 cggaaggtaa atcgcatact tttcattcta gaggagaagg tctcgttgat cattaactga
      601 ttaagcacca tagcccagtt ttgaattcga ccacctgacc acttcgcgtg taattctttc
      661 gtgcgtaaat aaagtagttt taggagtgca ttctcattag agaacgctcc tttttttgtg
      721 acttttctga agctggagtg gacactttca accgcattgg tagtgtacat aatttttcga
      781 atggcactac cataatcaaa tagttgttca acatgtgcaa agttccgttt ccagacatct
      841 acagcaccag aataatgaga ccaccgattt tgaaaactgt caaaagcagc atgtgcagcg
      901 tttagagaag aagcaccgta gaactttttc atatctcggc agacttcctt atagtcctta
      961 cttggaatat agcgcaatgc atttcgaaca agatgaacaa tacagcgctg aacaattacc
     1021 gatggaaaga tcgcttttgc gccttcttcg aggccagaaa caccgtccat cgaaatgaaa
     1081 aagacatctt cgacaccacg tgctttcagt tcatcaaata cttgcatcca gcgattttta
     1141 gattctgttt gatttaacca taatcctaaa atctccttat ttcctttgag atcatagcca
     1201 agaatggtgt atacagcata ttctttggct tcataatttt ctcgtaaagt aacatacata
     1261 caatcaacga atagaaaggc ataacacttt gctagggggc gggcttgcca ttcttccaat
     1321 tcaggaagga cagcgtcagt gatatctgaa atcatttcat gggaaatatc aaagccataa
     1381 atagcttcga cggttgcggc aatatctcgt tgactcattc ctcgtgcata catggaaaga
     1441 accttccctt cgatgtcgga gacatctcgt tttctcttag gaattaactc tggttcaaag
     1501 gaagcttccc ggtctctagg aacatcaata gctacttcac caaaactggt tttaagcgtt
     1561 ttagttccat agccatttcg acggttatcg tgttccttag gctctttaga atgggcatca
     1621 taacctaaat gattattcaa ttctccttga agcatttttt caaaaagggg cccaaacaca
     1681 tctttcaaag catcttgcat gtcatcgaca gattcaggtt gataggcatt cagaatggat
     1741 tcagctaact tttcggcatc aggatttctt ttctttctag ccatcgtgta atcacctctt
     1801 gatcttattg tagaaaaaga aaaaccgcga agcaaccacc tgcctaggat taatggttac
     1861 ttacacggtt tatattacac tctcatcatt aaaaaagtat ctttcgatat tactaaacaa
     1921 tgaaattatc tatttgaaat ttaaatctga taatatattg agtgttccaa tgagggcttt
     1981 gatcctatta tccatatctt tttcagtttt aatattgata cacattataa gaaaaagaaa
     2041 cttttggtta atttatttca tcccagtgca aaatataaaa aagtaaaata gaccataggc
     2101 aatagattaa caattaatat aatacaagca aatattctat tcattgtatt tctgctttca
     2161 aaaagttcta atattgaaag tataattcca caaatgccta taataagtat gggaataaga
     2221 ttagaaaata acaccccgag ccaatctgtg aaaattacta tatttactaa tatccctatt
     2281 aacataccta tcatatttat taaatcaatt tttttcatta gatatttctc ctttattaaa
     2341 tctgaagtac ttatattaaa ataattctaa actatttacc tcaaaaagtg caataaatta
     2401 tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt gttaatcgtt cagagccatc
     2461 cgagagt
//
LOCUS       DASHWH010000166         2458 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.176, whole genome shotgun sequence.
ACCESSION   DASHWH010000166 DASHWH010000000
VERSION     DASHWH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.176"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..132
                     /locus_tag="U2R41_002884"
     CDS             <1..132
                     /locus_tag="U2R41_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890210.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1342..1896
                     /locus_tag="U2R41_002885"
     CDS             1342..1896
                     /locus_tag="U2R41_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8890211.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2150..>2458
                     /locus_tag="U2R41_002886"
     CDS             2150..>2458
                     /locus_tag="U2R41_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890212.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          945 a          284 c          383 g          846 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 ctgtaatttt ctacgttttc cttttcatat atattaaata ttacggaaat ttatataaac
      241 taatttagtg ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc
      301 tatttaactc atcagacatt ctatgctcat tttctccaaa tttgtttcaa ctctttcgta
      361 cttaattttg cgtatttgaa aattcttctt ttagagtact tttattacga tactagtatg
      421 atatatatct atagcaaaca gttaaaatct tcctaaaata agcgaatagc attcctaaaa
      481 cctataaaat ttttagatga agcgacaagt ggacaattaa taaactactt gaacatgaaa
      541 ttatggatca cctttcaaat ttagaaaatg gaattcttat acttattgcg caacatttat
      601 tgattgcaaa attatgtgac aaaaagaact agtagaagaa ggaataatgg cttgataata
      661 aagtactacc tattaaaggt agtactttat ttaatttagt ataaattaac atactactat
      721 attttaagtt tcatattata taaattaata atatactacc attttttggt agtataattt
      781 aaaggtaata agttattctt ttattactat gtttgggttt tatagcaagg aaagtttaat
      841 aagagtttgg acctagcaaa caatattaat gaggtcatta aatcttatta tctttagtga
      901 tataacgaaa tatctacaca gacaaaatta tgatcttgcg taaagttata aaatattaat
      961 aaaaatattt tattaatgaa aagcttggta gcacggttat tcatatgcta atttatttca
     1021 ctttatagta aggggagttc ttagtatatt gtccaaattt aaatttcata taatttaatt
     1081 atgtaaatta gctattctaa gaacttctac ggataactgc ccgtctaaaa tttaattaag
     1141 agcttagaat cttcatataa gtaaggttaa tttttttgta caaaatagtc ggaatattct
     1201 gagttactga aatgatataa aatcataaac taaacccctt gattttcttc acttttaaca
     1261 gagtatatat ataagttcat gattatggta aaatatatat ataatcatga actcagaata
     1321 tttttaagaa gggaaacaat agtgagctat aatgtacaac cgttaaggac aaatgaagag
     1381 atacaagatt ttcagttttg gctaagaaga acaagtaatc cagaaagaga tagtttttta
     1441 tttttgtttg gaatcaataa tgggttacgt atgtcagata ttataggatt aaaagttgga
     1501 gacatacgag gtaaaagtaa gcctattatt gttgaacata aaacaggtaa gcgaaaaccg
     1561 atttttatag ataatttgag agaagagatt ttattatata ctgaaggtaa agaagaaaat
     1621 gattggttat ttccaagtcg gcaacaaggt cgacatataa caagggatcg tgtctatcag
     1681 atatatgcag atatagcaga aaaattagga agagatgata taggaacaca tacattaaga
     1741 aaaacttttg ggtatcacta ctacaagaaa actagagaca ttgctacctt aatgtttatt
     1801 tttaatcata gctcgcaagc aattacaaaa aggtacattg gtataactga agatgaaata
     1861 ggggcatcgt tacgtggttt taaattggga ggctaattga gatattagga tatatttttt
     1921 gtgtataatg aaaacttaga aggtgagatt aatgaagata actgaatttt cggaagtatt
     1981 taaaacgttt agtaacattt acaatagaat accagagata gaacaaaatg ttgtagttga
     2041 taatgattat tatatagaga atgattttaa taaaccaaga aaatatgaca acctatacaa
     2101 ttttatacac aatagaacaa caataaataa caacagtaac actcgagttt tgattaattc
     2161 agcatatttt catattaaat atttaaagaa atatgagtat gaagaagttg tattagatta
     2221 tgggctaaat agacagtcat ttttaattta tgcaataaat aacggaaatt tgccaagcaa
     2281 taaaaagaac ataattattg aaacatatgc ctatctggag gggggatcta aaaaactaat
     2341 taagtcatgt aatatagagt atcaattatc agtaggagaa attaaatgtg ttttcgaaga
     2401 agatttagaa cttgacttaa aaaaaatttt tgccagcatt catgattata taggtttg
//
LOCUS       DASHWH010000167         2397 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.177, whole genome shotgun sequence.
ACCESSION   DASHWH010000167 DASHWH010000000
VERSION     DASHWH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.177"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            206..2068
                     /locus_tag="U2R41_002887"
     CDS             206..2068
                     /locus_tag="U2R41_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8890213.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="U2R41_002888"
     CDS             2068..>2397
                     /locus_tag="U2R41_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8890214.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DASHWH010000168         2356 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.178, whole genome shotgun sequence.
ACCESSION   DASHWH010000168 DASHWH010000000
VERSION     DASHWH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.178"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..600
                     /locus_tag="U2R41_002889"
                     /pseudo
     CDS             1..600
                     /locus_tag="U2R41_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            634..2337
                     /locus_tag="U2R41_002890"
     CDS             634..2337
                     /locus_tag="U2R41_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890215.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQ
                     HNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
BASE COUNT          943 a          399 c          512 g          502 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaatga tcaaatcgaa
       61 aagatgtttg aacaccaaag aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg
      121 cattcttggt ttaggcaaca agaacgaatc gaaaataaag tccaaagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggttataaca agtacggtgg cctggatatg acgatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ctgccacgat ccgaaaatac
      361 caacgaaaat tggaaacatt agaacagctg aaagaaaaat cagagaaagg aaaagaaaat
      421 attcttcctg aagtacaagc gatcattgac agcggacgag tgacgcaatg gaaaaagaat
      481 ccaactatct attttctgaa aggctatcga aaagtcgctt tagaactcaa tgaaaacggg
      541 ggatttgagg aatccaaaaa ataccaagcc acgtcggaag aagaaaaggc agctgttcgt
      601 gaattacttg aagaagctac acctactgat tctgtgaagg agaaagaaac cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gcaaaagggt tgaaaagtta tggtggggag
      721 atcgtaacac tcgaattgat cgatcaactc aaaacactgg ataaccaata cgccgcagaa
      781 gcaggctatt ataaattcta ttttgaagaa gtgaagaatg gagaagtagt agatcagata
      841 cgaatggatc taggggatgg cgtcaatagt aatcacccca tctatttaga gttagctaga
      901 ttgtgcgaac aatcacctga aaaggaacaa gaacaaacag tcaaaaatga acctatttca
      961 aaagtacaaa atgagattaa agagacaaaa acaaaacaaa agggttatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatca ctttcccaac aggcgcttga tgcggtgaaa
     1081 aagcataccc attctcctga agacgtcaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatc gaggaacaat ttccaggcgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaaccg
     1441 gaagactatc cgaaaatctt cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg
     1501 cagacagaac ttcaagttgg gattcaagcg gtagccaaaa atattggaat tcaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcca caagtattca cgaattagcc catgctagaa tgcacaaatt ttcagaatta
     1741 gatactgcaa caaaagaact acaggcagaa atgacctcct atattgtgtg caagcatttt
     1801 gggatggaca cttcggagaa agctattcct tatattgcga catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagaacaa
     2041 aagaatattg tgcccgttgg ctcactttgg atcgacaaga aagacgggaa agtcctggaa
     2101 gaagacacgg gccgaacgtt acatttgaag gatgcggcgt ttattgaaga tccgcagcat
     2161 aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga ttcaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa tccattccga ttttcacttg aaacagtgtc agaagtggcc
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg cgtataaaac
     2341 aagtaaaaaa gactat
//
LOCUS       DASHWH010000169         2348 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.179, whole genome shotgun sequence.
ACCESSION   DASHWH010000169 DASHWH010000000
VERSION     DASHWH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.179"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            252..1550
                     /locus_tag="U2R41_002891"
     CDS             252..1550
                     /locus_tag="U2R41_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8890216.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISNVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2197..2348
                     /locus_tag="U2R41_002892"
                     /pseudo
     CDS             2197..2348
                     /locus_tag="U2R41_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          837 a          399 c          429 g          683 t
ORIGIN      
        1 tttagttttt cttggaaata aaaacagcga aaatccgaac attttcacct ctctcacttt
       61 ataactttta caaaatagta cttctcataa aaagcgctct agttaaagaa aataagagaa
      121 aaatcaaaaa aaacaaaagt ttcttttaca cttgtttcta tattcgttac aatggttatg
      181 ggaagttttt aaaaaggtta agaaaagctt acgggggttt ttgaaagcaa aaagtagtag
      241 gggacgataa aatgaaacat acacacaaaa tcatattggc tgtcacttct gtatgcactg
      301 ttattattct aggtgtaagt atcttttttg ttacacaagc agcaacaaac aataaattag
      361 aaaacactag tcaaacaact caacactcaa gcagcagctc aaaacctgtt gaagataaac
      421 aaacgacgaa acagcttgat caagcaaaac agcttgctgc aagttatcat tatgatgaag
      481 ccatcgcctt acttgaaaaa gacgacgcca aagaagcgca acaattgtta gcaactttga
      541 aaaaagaaaa agaatccttg gtaaaatggg aagacccgac caaaatctca aacgtcttct
      601 ttcatagtct gatcgttgat ccagctaagg cttttcatac ccagcaagca caagggtata
      661 aagattatat ggtcactatt tccgaattca ataaaaccat tgatcagtta tataaaaata
      721 actatgttct tgttaatcta aatggattag tcaaaaaagg aacagatgga aaactgacct
      781 ttacaggtgt ttctcttcca gaaggaaaga aaccgttaat tctttcgcaa gacgatgtca
      841 gctattatga atacatggac aattcaggat ttccaagtaa gttgattgtc gataaacaga
      901 accaaatcaa gaatatttat atcgacaata agaaggaaac tgttggtgac tatgatatgg
      961 ttccattgat cgattcattc attaaaaaac atcccgattt ttcttatcaa ggcgccaaag
     1021 gaacattagc tttaacaggc tataatggtg tattaggtta ccgtacatca aaatcagaat
     1081 atggtgataa cgaaaaaaca cacaaagaaa tcgaagcagc caaaaaagtt gctgatcaat
     1141 taaaggaaga tggttggagt tttgcttctc atacttgggg acacttgaat atgacacaag
     1201 cttctttagc cgatatccaa caagataatg aacgttggca aaatgaagtt gcacctattt
     1261 taggcaaaac aaatattttg atctacccat ttggcgctga catcagcgat tggcaacctt
     1321 attctgaagc aaatcaaaaa tttgcttact tgaaacaaca aggatttgat atcttctgca
     1381 atgtcgatgc ctcaactcct gcatggggac aactaggaac tgactactac cgtaacgcca
     1441 gaatcaatat tgacggtatc cgtttcgaag ctgacctaaa aggagaaaat ccaatacttg
     1501 atcactttat caacgtcaaa gaaatgtatg accaaaaaga tcgaggatag aggttgtgaa
     1561 agaagcgtcc agctccgagc aaataagaac tagatttcta aaatagtttc tcatattttg
     1621 gaaatctagc gcttattgct gaggagttgc ttcttgaaca ccgtgaatag aggttgtaaa
     1681 aagagcgttt tgacttattg ttgaaggtca gctctttgaa caccgcgtgt taggttgtga
     1741 aaaggctggt ctgcatcaag caataagaat gaacagagaa aaacagcttc ttgaataccg
     1801 cagataggtt gtaaaaaaac cgttttgacc tgacgataac cagtaaaaca gaggtgagac
     1861 cattgctaaa agtagcagtg gtctcatctc tttttttgca caagtcatat ttgattgttt
     1921 cgttatttta atcaggataa agttcttttt ttcatgctaa agagtgattc tttcttctcg
     1981 attttcactt tcttcgttta acttttggta aatacttctg tcagaaaaag ctctaggtag
     2041 acacacgaaa gcagataaaa agcctaactt ttgcaacagt ttatgctgtc gttcaaagtt
     2101 aggcttttac agtttataaa tagggttctg ttgcaaagtt ttccaaaaaa tctattttag
     2161 tgtaaaattg agaaaaaaga cagagaggac agagtaatga atcattttaa aggcaaacaa
     2221 ttcaaaaaag acgtcattat tgtcgctgtt ggttactacc tgcgttacaa tctaagctat
     2281 cgttaagttc aggaattgtt atatgatcgt ggaataaatg tttgtcatac tacgatttat
     2341 cgttgggt
//
LOCUS       DASHWH010000170         2331 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.180, whole genome shotgun sequence.
ACCESSION   DASHWH010000170 DASHWH010000000
VERSION     DASHWH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.180"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            287..694
                     /locus_tag="U2R41_002893"
     CDS             287..694
                     /locus_tag="U2R41_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890217.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="U2R41_002894"
     CDS             708..1109
                     /locus_tag="U2R41_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890218.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="U2R41_002895"
     CDS             1111..1482
                     /locus_tag="U2R41_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890219.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="U2R41_002896"
     CDS             complement(1518..1817)
                     /locus_tag="U2R41_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890220.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="U2R41_002897"
     CDS             complement(1823..2053)
                     /locus_tag="U2R41_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890221.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          843 a          316 c          389 g          783 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tctggcacgt cagtcacgat atcaaacaaa
     1621 aaatatacat ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taaactatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 catactttat aaaaatcttt gataaatcta ttcgaaggat caaatttaaa ccataattgc
     1801 gtctcattaa aaagcatagc cattactccg atctattttt aaacttagtt tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag ataatactgt ttcttttttc gcaattacag
     1921 gttggttatt ttcggaaaaa gctaatatag ctaagattga aacatccatc tggaatttat
     1981 cttttttgct gctttgctca taaaagtctg catattcatc actgatattt tttctaataa
     2041 agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc tcgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatacgttta cctttgatat atctcagatt
     2161 gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga gggtattaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg t
//
LOCUS       DASHWH010000171         2266 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.181, whole genome shotgun sequence.
ACCESSION   DASHWH010000171 DASHWH010000000
VERSION     DASHWH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.181"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..987
                     /locus_tag="U2R41_002898"
     CDS             28..987
                     /locus_tag="U2R41_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8890222.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1240..1389)
                     /locus_tag="U2R41_002899"
     CDS             complement(1240..1389)
                     /locus_tag="U2R41_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890223.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1851..2063
                     /locus_tag="U2R41_002900"
     CDS             1851..2063
                     /locus_tag="U2R41_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890224.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          817 a          358 c          394 g          697 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg ccgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagattat attaaatatt atacagataa
     1021 taacggaata ttatcatcga aacagctaac aaaattgtca taaaaaacga gatggttgat
     1081 attagaactt gtgtttttgt ttaggtctag agataaagag agacaatttc caaatatatg
     1141 taatgaatac aaaaagacgt ttgtcgcgga ttaccgttac aaacgtcttt tattgatttt
     1201 ccgagatctc atcagtcgcc atttggaatg gttcactcct tatctagatt cgtttaatgc
     1261 tcggactgct tgttcgattg taataccaaa cgcaacgtta ttttcgtttt gtgcatcaat
     1321 cgccataaaa cggttagaag ttgtatcaaa agctacgcgg tattgcatat ttgtttttgt
     1381 aaacgtcata aaattcatct cctttgtaat gtaatttagt tcttgaaagt ttcctttaaa
     1441 ctttcgaact tataccaagt ttagcatcgt acgttttttg atgcaattaa aaagcattta
     1501 tcattttcct tatttatttt ttcttaaatg gtttagctat tttcttttaa tccgataagt
     1561 agcccaatca tcaaggttac aaatactatt gcttgaatat aaatcatatg ctcacctctt
     1621 tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa tataaacaaa
     1681 cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat
     1741 actcaaaata aaaaataaaa aaatggataa aaacgttttc aaatacaagg taatttgtta
     1801 ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag
     1861 cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct
     1921 gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag
     1981 actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg
     2041 cacctgctga attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa
     2101 tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat
     2161 gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat
     2221 gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaa
//
LOCUS       DASHWH010000172         2233 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.182, whole genome shotgun sequence.
ACCESSION   DASHWH010000172 DASHWH010000000
VERSION     DASHWH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.182"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(51..1598)
                     /locus_tag="U2R41_002901"
     CDS             complement(51..1598)
                     /locus_tag="U2R41_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM8890225.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="U2R41_002902"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="U2R41_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEM8890226.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..>2233)
                     /locus_tag="U2R41_002903"
     CDS             complement(2043..>2233)
                     /locus_tag="U2R41_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890227.1"
                     /translation="KLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNG
                     INNYILQAILKELLSNAL"
BASE COUNT          626 a          465 c          397 g          745 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg tttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tat
//
LOCUS       DASHWH010000173         2194 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.183, whole genome shotgun sequence.
ACCESSION   DASHWH010000173 DASHWH010000000
VERSION     DASHWH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.183"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_feature    109..234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            278..802
                     /gene="infC"
                     /locus_tag="U2R41_002904"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="U2R41_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEM8890228.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="U2R41_002905"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="U2R41_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEM8890229.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="U2R41_002906"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="U2R41_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEM8890230.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2194
                     /locus_tag="U2R41_002907"
     CDS             1838..>2194
                     /locus_tag="U2R41_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM8890231.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          479 g          596 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DASHWH010000174         2189 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.184, whole genome shotgun sequence.
ACCESSION   DASHWH010000174 DASHWH010000000
VERSION     DASHWH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.184"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(325..528)
                     /locus_tag="U2R41_002908"
     CDS             complement(325..528)
                     /locus_tag="U2R41_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890232.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="U2R41_002909"
     CDS             complement(987..1952)
                     /locus_tag="U2R41_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM8890233.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DASHWH010000175         2181 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.185, whole genome shotgun sequence.
ACCESSION   DASHWH010000175 DASHWH010000000
VERSION     DASHWH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.185"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(118..708)
                     /locus_tag="U2R41_002910"
     CDS             complement(118..708)
                     /locus_tag="U2R41_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEM8890234.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="U2R41_002911"
     CDS             1231..1392
                     /locus_tag="U2R41_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8890235.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="U2R41_002912"
     CDS             1411..1905
                     /locus_tag="U2R41_002912"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8890236.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1898..2059
                     /locus_tag="U2R41_002913"
     CDS             1898..2059
                     /locus_tag="U2R41_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890237.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          792 a          333 c          370 g          686 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacgag agtataaaat
     2161 attttgtgta aatgaaaaaa t
//
LOCUS       DASHWH010000176         2123 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.186, whole genome shotgun sequence.
ACCESSION   DASHWH010000176 DASHWH010000000
VERSION     DASHWH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.186"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            18..1478
                     /locus_tag="U2R41_002914"
     CDS             18..1478
                     /locus_tag="U2R41_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM8890238.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKDIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRARMFQQGRQHERE"
     gene            1489..1932
                     /locus_tag="U2R41_002915"
     CDS             1489..1932
                     /locus_tag="U2R41_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEM8890239.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKSTRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPNQYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQ"
     gene            2008..>2123
                     /locus_tag="U2R41_002916"
     CDS             2008..>2123
                     /locus_tag="U2R41_002916"
                     /inference="COORDINATES: protein motif:HMM:NF013311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890240.1"
                     /translation="MIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQL"
BASE COUNT          755 a          368 c          453 g          547 t
ORIGIN      
        1 ttttgggagg tttgaaaatg accgagaaaa aaatgagtct tattgatcga tgtaaacaga
       61 tcgatatagt ggattttgct cgaaacaatg gaatggctgt agtcaataaa ggacgagact
      121 atcgcttaga agaccatgat tcttttgttt ttgatcgaag aaaacaacgt ttttattgga
      181 atagtcaaaa tattagtggc gacattattg aattagctaa actttttttc atagacaaag
      241 atatccaaga ccctaagcaa caatttaaag cagccactga ttttattcta aagaatgaag
      301 acaaaacaga acgagtggaa aatcttcatt ttgagacaga aaagtacaaa gatcaccccg
      361 ttgattatca accactcaca gaaaaaggaa ggaattatct gaaggaagaa cgaaaactgc
      421 ccgattggtt gattgattat gcagaaaaag aaggattgat tgccgaacta aagccaaaac
      481 atgaacggca aaatttctta gttcgagacg atcgtttgga tcatgcggtc gcctttttat
      541 ggaaagatcc acagacaaaa gagacagtag gggcgagtta tcaaggaacg ttcattgatt
      601 atgaacgttt tggtgagaga ggcacatata agcatataga taaaaattcg acagcgaatc
      661 atggctttaa cctaaagatt ggtgatccta aacagttaaa attttttgag agtagtattg
      721 atttacttag ctacgctgca ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta
      781 tggaagggtt aaaacaccat gtgatcagtc attattttgg tgaggcagta tctgaattga
      841 gaaagaaaca agcctttcct caatccatcg agatttgtgt ggataatgac cgtgcaggac
      901 atattttcta cgaaaaagaa caattaatgg gcgcagtcga tccatttacg aatcagaaag
      961 tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc aagagaatac aaagtcattt
     1021 atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc tattatggcc atacacaaga
     1081 cagaaaacaa tctgcaatta acgaaccaac tggtttccgc tcacaaagta aatgcgtctt
     1141 ttggccagca gctttctgtt aacgattcaa tagaagccat taatttgaaa gatatttgta
     1201 gagaagtcgc aaaagaatta aaagcttgtg aacgcgtgga tggtacgtac gattttgatc
     1261 gtttttatca agaaaaaggg gatattaatg cgcagattct tttctcttat aaagcagaac
     1321 aatactacaa aggctataaa aagcatgaac atgaatttgt acctgaagta aaaaaggact
     1381 ggaacgatca attgaagcat gagatccagc aacaagaaat cagaaagcaa aaacgtgcca
     1441 ggatgtttca acaaggtaga cagcatgaaa gggagtgaaa tcgtgatgat gaaaaccgtt
     1501 attttagcag aaaagccgtc acaagcgaaa gcttatgccg atagcttctc taaatccacc
     1561 cgaaaagacg ggtattttga gatccaagat cgattgtttc ctggtgaaac tgtgatcacg
     1621 tatggttttg gtcatttagt cgagttagat tcgcccgata tgtacgatga aaattggaaa
     1681 caatggtcat tagagcattt gcctattttc cccaatcaat accactatca tgttcctaaa
     1741 gataagaaaa agcaattcaa cgtggtgaaa cagcaacttc aatcggccga caccattatt
     1801 attgcgacag atagcgatcg tgaaggggaa ctgatcgctt ggacgatcat tcaacaagca
     1861 ggcgccgatc atggaaagat atttaaaaga ctttggatca actccttaga aaaagaagcc
     1921 atttatcagt gattccagca attacgagac gcagaagaga cctatcccaa atttgaagaa
     1981 gcacaagcac gccagatcgc tgattggttg atcggcatga acggcagccc actgtatagt
     2041 ttactcttgc agcaaaaagg cataccagga agtttttcct taggcagagt ccaaacccca
     2101 acgctttaca tgatttatca gct
//
LOCUS       DASHWH010000177         1975 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.188, whole genome shotgun sequence.
ACCESSION   DASHWH010000177 DASHWH010000000
VERSION     DASHWH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.188"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..202
                     /locus_tag="U2R41_002917"
     CDS             <1..202
                     /locus_tag="U2R41_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890241.1"
                     /translation="RKKPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIR
                     TYAWRNHTDRKGHEYKYLEEEK"
     gene            199..1014
                     /locus_tag="U2R41_002918"
     CDS             199..1014
                     /locus_tag="U2R41_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEM8890242.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            1153..1464
                     /locus_tag="U2R41_002919"
     CDS             1153..1464
                     /locus_tag="U2R41_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEM8890243.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            1461..1691
                     /locus_tag="U2R41_002920"
     CDS             1461..1691
                     /locus_tag="U2R41_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890244.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            1698..1901
                     /locus_tag="U2R41_002921"
     CDS             1698..1901
                     /locus_tag="U2R41_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEM8890245.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
BASE COUNT          734 a          302 c          470 g          469 t
ORIGIN      
        1 acgaaaaaag ccagacaaaa gaaaacagcg caaagggaaa atcgtcgttg ttttacacga
       61 aggcaaaatt ttgatgcaag gaacagctag tgagctgtct gcagaaactg gatatacgcg
      121 tgggactatt cgaacgtacg cttggcgaaa tcacaccgat cgaaaagggc acgaatataa
      181 gtatttggag gaagaaaaat gaacgaaaac aaattaatca aattgggtgt agcaggagca
      241 gtaatcgtag gtattggagt tatcggagga tttaagttct tcgaaaaaat cgataatgga
      301 tatgtgggtg tgcgctattc aatgaacggc ggtatcaaag atgaagcact gacgcaaggt
      361 gtgaaatttg tagggattga caaagtgatc caatatccaa ttcgcttgca aactatccaa
      421 tcaaaaaata tttcagtatc tacaagcgac ggcaaaaaga caacgattga tatcaaatat
      481 gactacaaag ttgattcaac taaagcagca aaaatgtaca aggaatttgg gaatatcact
      541 tcggaagata tcgaaagtgg atggttaaaa tctaagcttc aaaaggtcgc tcgtgaagtt
      601 tatgcgaaat atagtctgct tgatgtcctt tcaggagatt cctctaaagt tgaagctgag
      661 gtattaacga actttgctaa atcagttgaa tctaaagggt ttgaagtcga agacgtaaca
      721 cttggtgttc cagatgtcga taaagaaaca caaaaatcaa tcgatgcgat cattcgagct
      781 ggtcaagaaa atgaaaaagc gaagctagac gcagaaactg caaaaactca agctgatagt
      841 gaagcttaca agaaaacaaa agctgcagaa gcagaggcag aatctaatcg caaagtcgcc
      901 gaatcagtaa cagacaattt gattcgttat gaagaagctc aagctcgcaa aaagcatgga
      961 tgggtaacag taaatggagc agatactgta gttacggatg aagcaggcaa ataatatggg
     1021 attctttatg gctaaaattc tcttgttctt aggtttagta ggagcagcat atctcgtgta
     1081 tgcgctcctt tcccaaactg atgacaaaga agatgacaac aacgatgaaa tgaaatttta
     1141 ggaggagaaa taatggacga actaatcaca aaagtagagc agtgggctaa agacaaggga
     1201 ctggatcaag cagatccaaa agcacagttt ttgaaagtag ctgaggaatt cggggaaata
     1261 gcttcggcga tggcaagaag taatgatgaa ctatttaaag atagcgtagg agacgttatc
     1321 gtcacgctga ttatcctttc catgcaaaaa gggacaaacg tacaagagtg tttagaaatg
     1381 gcatacaacg aaatcaaagg acgcacaggg aaaatggtag atggtgtatt cgtgaagtcg
     1441 agtgatttgg aggatgtgaa atgaaatacg aaataccact aagtgaagcg ggcattcaag
     1501 caattatcaa tggtcgggag gttaacatag aacttcctga tggtactgaa ttagtcatca
     1561 gacaaagtta tttgaaagat atggcagctc cagtattaat tgatcgtttt aacgtgactg
     1621 attctgtggt agagaaccac ttaaaagaat ttcgatcaag tatagacaac actttcagat
     1681 tagggagttg attgacaatg aacaccagac accgcagagt agcaaaacta agaaaacagg
     1741 aactaaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca gaagaaacat
     1801 ataaagtggc aagtcagttt gttgctgatg caagtgatgc tattcgtaag tttgggattt
     1861 cgatattaaa tgatgatcgt aaatgggagg aaatgagatg aaactaaaag acggatttta
     1921 cgctagtagt cacggcatcg gcggtttaat gctagatatg ccgacaaaga accct
//
LOCUS       DASHWH010000178         1948 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.189, whole genome shotgun sequence.
ACCESSION   DASHWH010000178 DASHWH010000000
VERSION     DASHWH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.189"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            181..483
                     /locus_tag="U2R41_002922"
     CDS             181..483
                     /locus_tag="U2R41_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890246.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(999..1072)
                     /locus_tag="U2R41_002923"
     tRNA            complement(999..1072)
                     /locus_tag="U2R41_002923"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1627..1941)
                     /locus_tag="U2R41_002924"
     CDS             complement(1627..1941)
                     /locus_tag="U2R41_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890247.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          289 c          301 g          650 t
ORIGIN      
        1 ttttataagg gtttgagcat tttttatttt cgcagatttt cgttaaatga atgaaaatgg
       61 cattcaaaat agcattcaaa aagaacactt tttaatatat atgttccatc catattatca
      121 atgatattcc tactttacaa aaagaacatt agttcgtata ctctttttga ggtgaacatt
      181 atgttgatgg aaggaaaaac acaattatgg tttaaatttg atccttcgaa tagatttgtc
      241 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
      301 ttaatcaatc tctattactc taataagaac tactttaaaa ttccggcaac aaaaaccaga
      361 atgaagaaag atgtgtattt tttgtttgat atcgtgactg atgtgccaga cgcacggagc
      421 gatcatcggc gttatgacta tttgaagtat actttcgttg atccagaaag gtataaagat
      481 taaagtaggc tacctaaaaa aggtagcccg gaacggattt tatcaccata cttataaaag
      541 ggatattttt taagttagta ttaagattgt gtaatatgat gatatctata ttttatagta
      601 tcagtgctat aaaatcaaaa ataagtcact aattaactac cactccaatt ataagtcttt
      661 ttcccattat tttttacagt tatatggtat gctttttaat ggcttcaaat ataaaagagt
      721 ttaaagcgta acacacttat ggggaagtgg tttttgggga acgctttaaa ctcttcttta
      781 ttattatctc acaatataac ccaaatgtct ttccatttaa aaatcaaagt aaaacttttc
      841 aaatatacag aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata
      901 aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa
      961 aaaattctaa cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc
     1021 tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt
     1081 gccacataaa gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca
     1141 tatcttggag tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg
     1201 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     1261 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     1321 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     1381 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     1441 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     1501 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     1561 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     1621 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc
     1681 agaaataagg attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa
     1741 agaataaagt gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg
     1801 tgaatatacc cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccctgc
     1861 atttctactc ttatcaaaaa accaaatatt tagaagtaat aatattgcta ttattaagat
     1921 aaaattactg taaaaatcca aaagctat
//
LOCUS       DASHWH010000179         1856 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.190, whole genome shotgun sequence.
ACCESSION   DASHWH010000179 DASHWH010000000
VERSION     DASHWH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.190"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..153
                     /locus_tag="U2R41_002925"
     CDS             <1..153
                     /locus_tag="U2R41_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8890248.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="U2R41_002926"
     CDS             159..575
                     /locus_tag="U2R41_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890249.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="U2R41_002927"
     CDS             681..944
                     /locus_tag="U2R41_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8890250.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="U2R41_002928"
     CDS             944..1216
                     /locus_tag="U2R41_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM8890251.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="U2R41_002929"
     CDS             1293..1622
                     /locus_tag="U2R41_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8890252.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..>1856
                     /locus_tag="U2R41_002930"
     CDS             1612..>1856
                     /locus_tag="U2R41_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8890253.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          707 a          227 c          370 g          552 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtgg
//
LOCUS       DASHWH010000180         1786 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.191, whole genome shotgun sequence.
ACCESSION   DASHWH010000180 DASHWH010000000
VERSION     DASHWH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.191"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(35..403)
                     /locus_tag="U2R41_002931"
     CDS             complement(35..403)
                     /locus_tag="U2R41_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEM8890254.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            574..1530
                     /locus_tag="U2R41_002932"
     CDS             574..1530
                     /locus_tag="U2R41_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8890255.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1603..1719
                     /locus_tag="U2R41_002933"
     CDS             1603..1719
                     /locus_tag="U2R41_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEM8890256.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
BASE COUNT          656 a          318 c          276 g          536 t
ORIGIN      
        1 ttttaaaaga ctttttacag cttttctatt tttttcatag gtaagaaagt atgttcacaa
       61 tgatcaagac agcgtacaag acgattgtca acagaaaaac caaacgccaa gtcatcttga
      121 aataacgcgg ataaataatt tcttcataat aatatgcttg gaaaacagca atacaaattc
      181 ccattagcaa aacagaaata aagaaaaacg gtaagataga aattgtccct gtatttctag
      241 acaatgcatg cataccgccc cacaaaaaag gaatcgccaa atcaggggct tttattttaa
      301 aacgacgatt taacgaaaat aattttacaa gaaattggca accaaacaaa acgattaccg
      361 ggtaaatata ccaaaacaag ataagtggtg tgaatgtgcc catgaacgta aactcctttt
      421 ttatcgttgc ttcaagtata ctgtatatac tttacacaac tgtttcattt tatttaaatt
      481 tatgcagtta tattctatat cttacaaaca cattccatgt attccaattc cctatccgtt
      541 ataataaaag aaacaggcaa caggaggaaa atcatgatca aatattttac attagataat
      601 gagaaactgc gtaataccgg aaaggaaact gaccaaacga tctggtattg tgtagaacgt
      661 cctaatcaag aagagattca tcagctgaca catcaatttg aaattccgtc agattacatc
      721 acttccatat tggacgatgc agaaaattcc cgtacagaag aattcaatca atcaaagttg
      781 acaaagccag cgttattatt gttacaatat ccttttgcta aaacaagtcc tagcggttat
      841 tttcaagtgg atacataccc gctctctatt attttgacgc ccaaaaagaa gcttatcaca
      901 attacgaatc atgaaccact gtttttaaag aaagtcttta gccagacctt tccaaaaagt
      961 gatatttcgc ctaatctcaa tatcttcttt caattcattt ggcaggtcac actctcttac
     1021 aacagttatt tagctacgct tcaatctcaa tgcgatactt tggaagccaa acttcaagta
     1081 tccactgaaa acaaacaact ttatcaaatc atggatatcc aaaaaagcct cgtttatttc
     1141 agagaagcga cttctgctaa tttagatgca ttgaaagaac tagcgcaaaa taaacttttc
     1201 caagaaaatc atgctttgaa aaatcattta cgtgatgtat tggtggaaac agagcaggca
     1261 atgactactt ctcgcattca attaaaacta gtagaacaaa tgaatcagac tttttcagca
     1321 atcgtatcga ataatctgaa taatattatg aaaattttaa cttctttaac gattatttta
     1381 actataccga caatcgtcgg cagtatctat ggaatgaaca ttaagttacc tattgctgaa
     1441 agggatgatg cctttatatg gctagttcta ttaacattat ttatttgtat cgtaactacc
     1501 tattatttaa aaaaagggaa gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga
     1561 ttgtacactt gaatcaacta attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa
     1621 acaagtactt ttgctaaagt aacaaaattt gtcatttgga caatgctgat tttaacgatt
     1681 ggatctgttg tattgactgc tgtaatgagt gtcatgtaaa aataaaatat agctatcagc
     1741 tgctgaaaag gacctgaacc actgcgaggt ggctcaggtc cttttt
//
LOCUS       DASHWH010000181         1753 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.192, whole genome shotgun sequence.
ACCESSION   DASHWH010000181 DASHWH010000000
VERSION     DASHWH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.192"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(193..570)
                     /locus_tag="U2R41_002934"
     CDS             complement(193..570)
                     /locus_tag="U2R41_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890257.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(870..1751)
                     /gene="galU"
                     /locus_tag="U2R41_002935"
     CDS             complement(870..1751)
                     /gene="galU"
                     /locus_tag="U2R41_002935"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM8890258.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          504 a          381 c          254 g          614 t
ORIGIN      
        1 aaaacattgg tatgatttat atatatataa ataacataaa aaaaggaatg gcttatcccg
       61 atacaacaag cattccttta aaaaagattc ttttatataa gtgaaaaaag cttttttcca
      121 ccaattaaat aaatctctca tctcaatagt ttatccatta ttttatcttt ttttgcgtat
      181 acctactaaa tattaccgtc ttcttctcgt tcttctactt tgttcgagag aatctgtact
      241 agaaaaagta gttgtatgtc taccttttct gcctatccga gactttcttt tcatatatgg
      301 gatataaatg attagataca gtacagcaaa aaataaagca aatacgcaag cttcccaact
      361 acctaacgcc aattcaacca tcaaggtagc aaccccaacg gatgctgcat accagataaa
      421 aatcagaaac gtttcttttt ttctgaccat gcgaaacatg ataaatagac taataagcaa
      481 tagtggcaac acaaatatcc aattgcttcc ttccatcagc cacttgagta tatcaagcag
      541 cgactgtgca acagcgacta attttcccat agtgaacaca cacctttttt tataattaaa
      601 aaattcgcct ttttattcta ctatgaatct tctggcttgg ctttcatttc tcttatgtaa
      661 tcattttctt ttatacaact taaaaaaaaa ttaaaccatt agcataatat tttctgaatc
      721 tattcacgaa agtctttttc tatctttcgt tttccacgac tcaactgatc tagagtcaaa
      781 aaactaaaac gacagttgcg caaatgacgc acaagctgcc gctttaataa caaatagcct
      841 ttcattctca gcaggccact tatttccagt taactttctg ttcttagctt tttactcaac
      901 tggataatat atgcacttaa tgaagtccca atttccgaat gttgaagtcc atattcaata
      961 tttgtcttca tatatcctaa cttatcgcct acgtcgtatc tttttccttt aaactctctc
     1021 gcatagaccg cctctgtctg gttaagagtc tgaagtgcat ccgttagctg tatctcgttt
     1081 cctgctcctg gttcttgagt ttctaatatc tcaaatattt taggagtcag tagataacgt
     1141 ccgataatgg ctaaattact tggtgctttt cccatttcag gtttttctac aaaccctttt
     1201 actctgcaac gacccttttc gctatctgtt ttaggatcaa taattccgta tttagatgtt
     1261 tcattttctg gtacacgcat gacggcaaca gcggaagctt gtgtttcttc ataatccatc
     1321 atcaattgtt gagtcaatgg gatatcatct ttcatcaaat catcccccag catgacgaca
     1381 aacggttcat ttcctataaa tgcttttgct tgaagcacgg catcccccag tcctcttgga
     1441 tgagattgac ggatgaagtg aagattgact tgagtcgttt cttcaactaa agcgagtaat
     1501 tctgtttttt ccttttcccg caaattgttt tccaattcga tatttgcatc aaagtgatct
     1561 tcaatgggtc ttttttcttt tcccgttacg atcaagatgt cttcgattcc cgaatccaat
     1621 gcttcttcca caataaactg gatcgttggt ttatccacga ttggaagcat ttcttttgcc
     1681 atggcttttg ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat gactgctttt
     1741 ttgaccttca ctt
//
LOCUS       DASHWH010000182         1747 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.193, whole genome shotgun sequence.
ACCESSION   DASHWH010000182 DASHWH010000000
VERSION     DASHWH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.193"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..507
                     /locus_tag="U2R41_002936"
     CDS             133..507
                     /locus_tag="U2R41_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8890259.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="U2R41_002937"
     CDS             complement(621..1250)
                     /locus_tag="U2R41_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8890260.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="U2R41_002938"
     tRNA            complement(1384..1456)
                     /locus_tag="U2R41_002938"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="U2R41_002939"
     tRNA            1670..1743
                     /locus_tag="U2R41_002939"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
BASE COUNT          603 a          256 c          307 g          581 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaa
//
LOCUS       DASHWH010000183         1745 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.194, whole genome shotgun sequence.
ACCESSION   DASHWH010000183 DASHWH010000000
VERSION     DASHWH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.194"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            378..1628
                     /locus_tag="U2R41_002940"
     CDS             378..1628
                     /locus_tag="U2R41_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM8890261.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          334 c          308 g          526 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat gacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catct
//
LOCUS       DASHWH010000184         1701 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.195, whole genome shotgun sequence.
ACCESSION   DASHWH010000184 DASHWH010000000
VERSION     DASHWH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.195"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..255
                     /locus_tag="U2R41_002941"
     CDS             <1..255
                     /locus_tag="U2R41_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890262.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="U2R41_002942"
                     /pseudo
     CDS             258..411
                     /locus_tag="U2R41_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="U2R41_002943"
     CDS             434..1639
                     /locus_tag="U2R41_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8890263.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DASHWH010000185         1638 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.196, whole genome shotgun sequence.
ACCESSION   DASHWH010000185 DASHWH010000000
VERSION     DASHWH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.196"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(185..1504)
                     /locus_tag="U2R41_002944"
     CDS             complement(185..1504)
                     /locus_tag="U2R41_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEM8890264.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          526 t
ORIGIN      
        1 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
       61 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
      121 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
      181 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
      241 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
      301 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
      361 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
      421 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
      481 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
      541 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
      601 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
      661 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
      721 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
      781 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
      841 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
      901 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
      961 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
     1021 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
     1081 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
     1141 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
     1201 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
     1261 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
     1321 catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg ttgcttagcc agttcagaaa
     1381 aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc gataattgac
     1441 cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg tgtaagctag
     1501 tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag cagaaagggg
     1561 ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg ccgtgaggca
     1621 gtttcacatg gttttaat
//
LOCUS       DASHWH010000186         1579 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.197, whole genome shotgun sequence.
ACCESSION   DASHWH010000186 DASHWH010000000
VERSION     DASHWH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.197"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..1297
                     /locus_tag="U2R41_002945"
     CDS             128..1297
                     /locus_tag="U2R41_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8890265.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          566 a          254 c          258 g          501 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataat
//
LOCUS       DASHWH010000187         1558 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.198, whole genome shotgun sequence.
ACCESSION   DASHWH010000187 DASHWH010000000
VERSION     DASHWH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.198"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            553..>1558
                     /locus_tag="U2R41_002946"
     CDS             553..>1558
                     /locus_tag="U2R41_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HEM8890266.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     K"
BASE COUNT          555 a          293 c          267 g          443 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt aggcacgcta
      781 gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt tgaacgttat
      841 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
      901 actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      961 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
     1021 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgagaagaa
     1081 aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga tggcgaccgt
     1141 gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac aacatttttt
     1201 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
     1261 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
     1321 cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc tttcagagaa
     1381 gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc taaaaatcga
     1441 ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc attggatgat
     1501 ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg actaaaga
//
LOCUS       DASHWH010000188         1519 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.199, whole genome shotgun sequence.
ACCESSION   DASHWH010000188 DASHWH010000000
VERSION     DASHWH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.199"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(329..628)
                     /locus_tag="U2R41_002947"
     CDS             complement(329..628)
                     /locus_tag="U2R41_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890267.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(634..864)
                     /locus_tag="U2R41_002948"
     CDS             complement(634..864)
                     /locus_tag="U2R41_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890268.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1114..1314)
                     /locus_tag="U2R41_002949"
     CDS             complement(1114..1314)
                     /locus_tag="U2R41_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8890269.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          539 a          244 c          223 g          513 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccactgcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata tttttgaaaa aaagtgagaa aaaaggctta taattggagt ggtagttaat
      181 tagtgactta tttttgattt tatagcactg atactataaa atatagatat catcatatta
      241 cacaatctta atactaactt aaaaaatatc tcctttcata agtatggtga taaaatccgt
      301 tccgggctac ctttttaggt agcctacttt aatctttgta tctttctgga tcaacgaaag
      361 tatactttat atagtcataa cgccgatgat cgcttcgagc gtctagcaca tcagtcacga
      421 tatcaaacaa aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt
      481 agttcttatt agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa
      541 cttctgaatc ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa
      601 accataattg cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt
      661 ctctacctct aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt
      721 tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat
      781 ctggaatttg tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt
      841 ttttctaata aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt
      901 ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa
      961 attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga
     1021 taacatttct tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac
     1081 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
     1141 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
     1201 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
     1261 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
     1321 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
     1381 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
     1441 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
     1501 tcaaagtacc atataaaaa
//
LOCUS       DASHWH010000189         1456 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.201, whole genome shotgun sequence.
ACCESSION   DASHWH010000189 DASHWH010000000
VERSION     DASHWH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.201"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            108..1040
                     /locus_tag="U2R41_002950"
     CDS             108..1040
                     /locus_tag="U2R41_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEM8890270.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="U2R41_002951"
     CDS             1112..>1456
                     /locus_tag="U2R41_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890271.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DASHWH010000190         1451 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.202, whole genome shotgun sequence.
ACCESSION   DASHWH010000190 DASHWH010000000
VERSION     DASHWH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.202"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..1406
                     /locus_tag="U2R41_002952"
     CDS             105..1406
                     /locus_tag="U2R41_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8890272.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DASHWH010000191         1419 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.203, whole genome shotgun sequence.
ACCESSION   DASHWH010000191 DASHWH010000000
VERSION     DASHWH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.203"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            94..1389
                     /locus_tag="U2R41_002953"
     CDS             94..1389
                     /locus_tag="U2R41_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8890273.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          280 c          266 g          423 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa aacattttta
     1381 accaaataaa cagaacagaa agaggttttc ttctttctg
//
LOCUS       DASHWH010000192         1416 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.204, whole genome shotgun sequence.
ACCESSION   DASHWH010000192 DASHWH010000000
VERSION     DASHWH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.204"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(150..1337)
                     /locus_tag="U2R41_002954"
     CDS             complement(150..1337)
                     /locus_tag="U2R41_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEM8890274.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          307 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgctt
//
LOCUS       DASHWH010000193         1385 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.205, whole genome shotgun sequence.
ACCESSION   DASHWH010000193 DASHWH010000000
VERSION     DASHWH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.205"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1307
                     /locus_tag="U2R41_002955"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="U2R41_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEM8890275.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          595 a          103 c          233 g          454 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagt
//
LOCUS       DASHWH010000194         1362 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.206, whole genome shotgun sequence.
ACCESSION   DASHWH010000194 DASHWH010000000
VERSION     DASHWH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.206"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1362
                     /locus_tag="U2R41_002956"
     CDS             82..>1362
                     /locus_tag="U2R41_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEM8890276.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          462 a          255 c          259 g          386 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DASHWH010000195         1326 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.207, whole genome shotgun sequence.
ACCESSION   DASHWH010000195 DASHWH010000000
VERSION     DASHWH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.207"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1326
                     /locus_tag="U2R41_002957"
     CDS             82..>1326
                     /locus_tag="U2R41_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEM8890277.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          449 a          258 c          253 g          366 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgag
//
LOCUS       DASHWH010000196         1292 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.208, whole genome shotgun sequence.
ACCESSION   DASHWH010000196 DASHWH010000000
VERSION     DASHWH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.208"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..398
                     /locus_tag="U2R41_002958"
     CDS             141..398
                     /locus_tag="U2R41_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890278.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="U2R41_002959"
     CDS             complement(630..1061)
                     /locus_tag="U2R41_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEM8890279.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          167 c          161 g          475 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca cacataaaaa taaaaaacat
     1141 ttgtatttta tcaaagtttt attatacttg tcaatttgtg tacatcctaa aatataagct
     1201 atttgtttac ttctgtagaa tatatacttt gttgttagat aagttgcaca tgtttataga
     1261 atctaaataa taaattttta tgtaccgccc ct
//
LOCUS       DASHWH010000197         1287 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.209, whole genome shotgun sequence.
ACCESSION   DASHWH010000197 DASHWH010000000
VERSION     DASHWH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.209"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            316..570
                     /locus_tag="U2R41_002960"
     CDS             316..570
                     /locus_tag="U2R41_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8890280.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="U2R41_002961"
     CDS             571..888
                     /locus_tag="U2R41_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8890281.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          449 a          208 c          255 g          375 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatg
//
LOCUS       DASHWH010000198         1270 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.210, whole genome shotgun sequence.
ACCESSION   DASHWH010000198 DASHWH010000000
VERSION     DASHWH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.210"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            61..732
                     /gene="radC"
                     /locus_tag="U2R41_002962"
     CDS             61..732
                     /gene="radC"
                     /locus_tag="U2R41_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEM8890282.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(627..>1270)
                     /locus_tag="U2R41_002963"
     CDS             complement(627..>1270)
                     /locus_tag="U2R41_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEM8890283.1"
                     /translation="KKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVAGQTIK
                     ARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKFEQIQI
                     NYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNEIEVEP
                     AKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI"
BASE COUNT          375 a          208 c          209 g          478 t
ORIGIN      
        1 aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt
       61 ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa
      121 atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt
      181 caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg
      241 gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg
      301 aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa
      361 agagcggctc caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg
      421 aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc
      481 ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt
      541 ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttcc
      601 ggaatatata agttagtaca cataaattat ataaggaaat ttactgttat attgccatct
      661 ggaaatacta ttatgttatc gataattgaa cgccataatg catttttttc ttcttccgaa
      721 gattctttat aaatttcatc gaattctttt gatagaataa attcgacatt ttttatatct
      781 attttagctg gctctacttc aatttcattt aattctttca ttaaagcttc tcgatctttt
      841 ttgtattcct ctaagtctat aacttcattc aagtaggctt tcttcaatct atctaattta
      901 ttctcaatat ttttcttact atttttcttt ttaataatct ttggttgttt ttttgaatag
      961 tttatttgta tttgttcaaa tttttgttta gtagcttcaa aaatataacg ttctaaattt
     1021 ttttcgtaaa aagcgcgctt atttgtgcaa cgtaatttat tatttcttcg ttttacacat
     1081 ctataacaag tcctttcgta tatagattct gtaccgtctt ttttcttata tcttgcttta
     1141 atagtttgac cagctactga tccctgacag tcagaacatt ttaataaacc tttaaaaatg
     1201 taatcacgtt ttgcatttgt ttttaaattt ttaggtaagg ctttttgaac agcataaaaa
     1261 agttttttat
//
LOCUS       DASHWH010000199         1246 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.211, whole genome shotgun sequence.
ACCESSION   DASHWH010000199 DASHWH010000000
VERSION     DASHWH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.211"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..742
                     /locus_tag="U2R41_002964"
     CDS             200..742
                     /locus_tag="U2R41_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890284.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="U2R41_002965"
     CDS             898..>1246
                     /locus_tag="U2R41_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEM8890285.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          237 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DASHWH010000200         1234 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.212, whole genome shotgun sequence.
ACCESSION   DASHWH010000200 DASHWH010000000
VERSION     DASHWH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.212"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            33..1211
                     /locus_tag="U2R41_002966"
     CDS             33..1211
                     /locus_tag="U2R41_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEM8890286.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          454 a          230 c          206 g          344 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      601 ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc
      661 tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct
      721 taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt
      781 gtatccatgt atctcgtaac atcgctcata aagtacgtgt caaagaccga aaagaaatct
      841 gtgatgactt taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat
      901 ccggcatgat tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa
      961 accaagactt attaactttt tatgattttc cacctagcat tcgtagaacc atttactcaa
     1021 ccaatctaat cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt
     1081 ttcaaaatga agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa
     1141 aatttctaaa cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt
     1201 ttactgagta actaattatt ttgcaggagg acaa
//
LOCUS       DASHWH010000201         1215 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.213, whole genome shotgun sequence.
ACCESSION   DASHWH010000201 DASHWH010000000
VERSION     DASHWH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.213"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            414..947
                     /locus_tag="U2R41_002967"
     CDS             414..947
                     /locus_tag="U2R41_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEM8890287.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DASHWH010000202         1061 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.214, whole genome shotgun sequence.
ACCESSION   DASHWH010000202 DASHWH010000000
VERSION     DASHWH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.214"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(36..992)
                     /locus_tag="U2R41_002968"
     CDS             complement(36..992)
                     /locus_tag="U2R41_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890288.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DASHWH010000203         1049 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.215, whole genome shotgun sequence.
ACCESSION   DASHWH010000203 DASHWH010000000
VERSION     DASHWH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.215"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..339)
                     /locus_tag="U2R41_002969"
     CDS             complement(<1..339)
                     /locus_tag="U2R41_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890289.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="U2R41_002970"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="U2R41_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEM8890290.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          315 a          196 c          170 g          368 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag ttgtcggtct
      841 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
      901 ggcgacttct gcctaattct gttaaaacga tgagggagat tgcctccctt acattttatt
      961 ttgtcgatcc tgtttcctaa tctttcgaca ctaccataat atcactgata aatggctaaa
     1021 aaccgccatc attccgccaa aaaaccgcc
//
LOCUS       DASHWH010000204         1030 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.216, whole genome shotgun sequence.
ACCESSION   DASHWH010000204 DASHWH010000000
VERSION     DASHWH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.216"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..931
                     /locus_tag="U2R41_002971"
     CDS             <1..931
                     /locus_tag="U2R41_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEM8890291.1"
                     /translation="EFNLGANEGSSQVANNQYILLHETANETATGRNEAQYMQRSWTS
                     AYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKAYVE
                     LARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYD
                     LAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHI
                     ANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKV
                     YVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          354 a          187 c          209 g          280 t
ORIGIN      
        1 tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc agtatatttt
       61 actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt atatgcaacg
      121 ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg tttatcaagt
      181 cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcca acagtcctgt
      241 gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact acaaggcata
      301 cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacgt tggacactcc
      361 ttataaccaa ccgggaatca aatcgcattt atgggtaaca caaaacatct ggggcgatca
      421 tacagatcct tacggttatc tttctgaaat gggcgtaagt aaagaaaaat tagcatatga
      481 tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc ctgtcattga
      541 tccaacacga gctggtgcag ctaatcctac actgacagat ggaacgaatt acgcccacat
      601 tgatcagttt ggagaaatcg aaaatgcaaa tttgcatgta gctggatggc acattgctaa
      661 ctataaatac gagtatatct tcattatgga ttacaatact gggaaagaat tagctcgagt
      721 aagagctgat ggaatttata gatcagatgt aaatcaagct tataatactt ctggaaatgt
      781 tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct atgtcatgat
      841 gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt tccatgacaa
      901 acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg agcattacat
      961 aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg catatcttca
     1021 ctatcaccca
//
LOCUS       DASHWH010000205         1016 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.217, whole genome shotgun sequence.
ACCESSION   DASHWH010000205 DASHWH010000000
VERSION     DASHWH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.217"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1016
                     /locus_tag="U2R41_002972"
     CDS             <1..>1016
                     /locus_tag="U2R41_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HEM8890292.1"
                     /translation="QKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGV
                     QLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYL
                     SYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAII
                     PTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGK
                     VPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLI
                     TAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGI
                     LLCQA"
BASE COUNT          388 a          191 c          224 g          213 t
ORIGIN      
        1 caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg cgcatttgat
       61 gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga ttatttaaaa
      121 gaaaaggggg ttcaattggg aaaacagcca ggtacgatcc accaagttga aaccgagaaa
      181 aaaagtgcgg caagtccacg tcttttttct ctatccagcc ttcaatctaa aatgaatcag
      241 ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta tgaagggaaa
      301 tatctaagct acccacgaac agatacccct tatattacag aaggggagta tgcgtatctg
      361 ttggatcact tagacgaata caaacacttt ttaaaggccg aagccattcc tactcctatt
      421 catacaccga atagtcgata tgtgaataat aagaaagtcc aggaacatta cgcgatcatt
      481 cctacaaaga cggtcatgac ggcagcggct tttgaacaat taagtccact gcagcaggct
      541 atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta tacctatgaa
      601 gaaacaacga tcttgacgca agttcagcag cttcagctaa agacagtcgg aaaagtacca
      661 gtagatctag gttggaaaaa gctgtttggc aaagagagcg acagaaaaga aaaggaagaa
      721 gaaccgctac ttcctaaagt gacaaaagga gaagcggtga cggtagatct acaagtcctt
      781 gaaaaagaaa cgaagccacc acaaccttat accgaaggta cgttgatcac agcgatgaaa
      841 acagctggaa aaaccgtgga tagcgaagaa gcacaatcca tattgaaaga agtagaagga
      901 atcggtacag aagcaacacg agcaaatatt attgaaactt tgaaacaaaa ggaatatatc
      961 aaggtagaaa aaaataagct tgtggtgaca aataaaggga ttttactttg ccaagc
//
LOCUS       DASHWH010000206         1000 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.218, whole genome shotgun sequence.
ACCESSION   DASHWH010000206 DASHWH010000000
VERSION     DASHWH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.218"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            369..1000
                     /locus_tag="U2R41_002973"
                     /pseudo
     CDS             369..1000
                     /locus_tag="U2R41_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DASHWH010000207          863 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.219, whole genome shotgun sequence.
ACCESSION   DASHWH010000207 DASHWH010000000
VERSION     DASHWH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.219"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..861
                     /locus_tag="U2R41_002974"
     CDS             <1..861
                     /locus_tag="U2R41_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEM8890293.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DASHWH010000208          827 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.220, whole genome shotgun sequence.
ACCESSION   DASHWH010000208 DASHWH010000000
VERSION     DASHWH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.220"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>827)
                     /locus_tag="U2R41_002975"
     CDS             complement(<1..>827)
                     /locus_tag="U2R41_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8890294.1"
                     /translation="IAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPKVLCFDEFKSVRG
                     VSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSL
                     VKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLL
                     QKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYV
                     SFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNA"
BASE COUNT          240 a          156 c          170 g          261 t
ORIGIN      
        1 tgcatttcgt tttaacactt tgatatggtt gttcagacat tcaagtgggc catttgaata
       61 actttgttgt aaagcatttt tgatttgtgg cagaaatttc tggaacgttc tcaaggtcgt
      121 ctgatagcct tcgggaagca tgatattatt ggtaagtagt tcttcaaacg atacgtagtc
      181 tcgttccttt acagcgtaaa gaaagtcttg atataactga tagcctacac gcaaagcagg
      241 gctccctttc agtaagcgat ccacaatatc ggtctctgtt aaatgagctt tgaagccagg
      301 tcgccaaagg cgtcgagtat aatcaagctt gcgttcatct ttttgaagaa gtttccagta
      361 tctttttaat tgcttaccta attgatagtg tttttgttct ttgcttttta atagttggtt
      421 cgtttctttg attcgatgat tgcgaaaggt acgaccaata tgttggacga tgtgaaaccg
      481 gtcaataatc agctgtgctt ttggaaacag ctttttaacg agcgatacat aaggagcgta
      541 catatcaatc acgatccatt cgaccgcttc acgaatttct cgtgaaaaat gactaaaata
      601 acgttttaaa aaaggcagtt gtcggttctc gacaatgtcc aataagcgct gtgtttgtcc
      661 atccatcatg atgaaactca tagcacctga aacaccccga actgatttga attcatcaaa
      721 acaaagtacc ttaggtaacg gtgtccgaaa aggattcaac ggacgataaa atcgcttcat
      781 aatacggtaa acagaagaga ccgatagatt tttctgagcg gcgatct
//
LOCUS       DASHWH010000209          823 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.221, whole genome shotgun sequence.
ACCESSION   DASHWH010000209 DASHWH010000000
VERSION     DASHWH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.221"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..>823
                     /locus_tag="U2R41_002976"
     CDS             22..>823
                     /locus_tag="U2R41_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8890295.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DASHWH010000210          789 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.222, whole genome shotgun sequence.
ACCESSION   DASHWH010000210 DASHWH010000000
VERSION     DASHWH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.222"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..782
                     /locus_tag="U2R41_002977"
     CDS             <1..782
                     /locus_tag="U2R41_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8890296.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DASHWH010000211          785 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.223, whole genome shotgun sequence.
ACCESSION   DASHWH010000211 DASHWH010000000
VERSION     DASHWH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.223"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..672
                     /locus_tag="U2R41_002978"
     CDS             <1..672
                     /locus_tag="U2R41_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890297.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          290 a          121 c          146 g          228 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccact
//
LOCUS       DASHWH010000212          775 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.224, whole genome shotgun sequence.
ACCESSION   DASHWH010000212 DASHWH010000000
VERSION     DASHWH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.224"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(249..728)
                     /locus_tag="U2R41_002979"
     CDS             complement(249..728)
                     /locus_tag="U2R41_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890298.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDTHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a          106 c           88 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtgtgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DASHWH010000213          593 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.225, whole genome shotgun sequence.
ACCESSION   DASHWH010000213 DASHWH010000000
VERSION     DASHWH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.225"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..580
                     /locus_tag="U2R41_002980"
     CDS             <1..580
                     /locus_tag="U2R41_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM8890299.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DASHWH010000214          588 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.226, whole genome shotgun sequence.
ACCESSION   DASHWH010000214 DASHWH010000000
VERSION     DASHWH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.226"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>588
                     /locus_tag="U2R41_002981"
     rRNA            <1..>588
                     /locus_tag="U2R41_002981"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          146 a          130 c          189 g          123 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacg
//
LOCUS       DASHWH010000215          575 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.227, whole genome shotgun sequence.
ACCESSION   DASHWH010000215 DASHWH010000000
VERSION     DASHWH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.227"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            312..>575
                     /locus_tag="U2R41_002982"
     CDS             312..>575
                     /locus_tag="U2R41_002982"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEM8890300.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DASHWH010000216          554 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.228, whole genome shotgun sequence.
ACCESSION   DASHWH010000216 DASHWH010000000
VERSION     DASHWH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.228"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..172
                     /locus_tag="U2R41_002983"
     CDS             <1..172
                     /locus_tag="U2R41_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEM8890301.1"
                     /translation="ISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFA
                     EAYKHGRIIAYK"
     gene            185..>554
                     /locus_tag="U2R41_002984"
     CDS             185..>554
                     /locus_tag="U2R41_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890302.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLC"
BASE COUNT          192 a           73 c          134 g          155 t
ORIGIN      
        1 gattagtcgt cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag
       61 gattagccgt gaagtgggtt attcagaaag aagcatacaa aggatgaagt cggaagctct
      121 aatagagttt gcagaagcgt ataaacacgg aagaataatt gcttataaat agggggacca
      181 tataatgtgg aaagattatg tatcgttgaa agagttgaaa aaagatcttg ttttcaaaag
      241 aattgtagaa tggtcagaga gtgaattaat tttagaagat ggaaccaaaa tggaagttgt
      301 atgtagcgaa tcagattgct gcgcatgggc cgaaggtgaa ttcaaaaatg taaagcttga
      361 tgcagtgatt acagatataa agatttttga taaaggtaac cgtctctata atggtgacgg
      421 acatacctct tatgctgaag tcgttgttta tcataatcgt aatgagatcg ctaaagcaga
      481 atgcacagcg aatgatggga atggcggcta ctattatagt gtttgtgctt taaaggtcaa
      541 agacaaactt tgta
//
LOCUS       DASHWH010000217          548 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.229, whole genome shotgun sequence.
ACCESSION   DASHWH010000217 DASHWH010000000
VERSION     DASHWH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.229"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>548)
                     /locus_tag="U2R41_002985"
     CDS             complement(<1..>548)
                     /locus_tag="U2R41_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8890303.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DASHWH010000218          546 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.230, whole genome shotgun sequence.
ACCESSION   DASHWH010000218 DASHWH010000000
VERSION     DASHWH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.230"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(271..534)
                     /locus_tag="U2R41_002986"
     CDS             complement(271..534)
                     /locus_tag="U2R41_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEM8890304.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           97 c           75 g          161 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctct
//
LOCUS       DASHWH010000219          528 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.231, whole genome shotgun sequence.
ACCESSION   DASHWH010000219 DASHWH010000000
VERSION     DASHWH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.231"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(5..90)
                     /locus_tag="U2R41_002987"
     tRNA            complement(5..90)
                     /locus_tag="U2R41_002987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U2R41_002988"
     tRNA            complement(107..178)
                     /locus_tag="U2R41_002988"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U2R41_002989"
     tRNA            complement(190..262)
                     /locus_tag="U2R41_002989"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U2R41_002990"
     tRNA            complement(280..361)
                     /locus_tag="U2R41_002990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U2R41_002991"
     tRNA            complement(369..441)
                     /locus_tag="U2R41_002991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U2R41_002992"
     tRNA            complement(445..517)
                     /locus_tag="U2R41_002992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASHWH010000220          528 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.232, whole genome shotgun sequence.
ACCESSION   DASHWH010000220 DASHWH010000000
VERSION     DASHWH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.232"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>528)
                     /locus_tag="U2R41_002993"
     CDS             complement(<1..>528)
                     /locus_tag="U2R41_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEM8890305.1"
                     /translation="HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNR
                     KAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVL
                     DRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAF
                     GRIPYSLTDVKLFHTD"
BASE COUNT          142 a          114 c          102 g          170 t
ORIGIN      
        1 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
       61 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      121 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      181 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      241 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      301 tccgcggcgt ttcatgattc gaccgattcg gcgccgactg agttgaagcc cacgctttgc
      361 taaacacttc ttcaattttc gggtaccgta agcctttcgg tttcgaataa actcttcctg
      421 aacgatctct tctaagtcgg actcattttc gatcggtttc gcttgataat agtaggtttg
      481 acgagaaata tttagaattt tgcacatcgc tgatatggaa tatttatg
//
LOCUS       DASHWH010000221          515 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.233, whole genome shotgun sequence.
ACCESSION   DASHWH010000221 DASHWH010000000
VERSION     DASHWH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.233"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>515)
                     /locus_tag="U2R41_002994"
     CDS             complement(<1..>515)
                     /locus_tag="U2R41_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8890306.1"
                     /translation="GEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPADMSFEM
                     YDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAIVV
                     LQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEIYG
                     EHLIKNVVLKNA"
BASE COUNT          159 a          100 c           82 g          174 t
ORIGIN      
        1 gcatttttga gtacaacatt tttaatcaaa tgttccccat aaatctcttg ggcttgttta
       61 tacatttcct gatctactgc gtcaccgctt tccaacatga aaggaataac accagcaaca
      121 tataaatttg tatcaaattc tttcacaaaa aagtccatat actcaagata ttgcatcgca
      181 tttctcatcg attttacttg ggtctgtaag acgacgattg cccagtccgc aatgtacatc
      241 gcggatttac tatatacaga aatagaaggt gggacatcta aatagatgac gtcaaactca
      301 tcaaataatg gttctagctt ttctttcata tatttgaatt gggataatga atcattatat
      361 tggtctttga tccaatcatc atacatttca aaggacatgt ctgctggaag tatatagagg
      421 ttattgatta ctttatagac agcctcttta ggtggaagat tgtttatcaa cacgtcagtc
      481 acagtatttt cgtaaacaat cggttcacct tctcc
//
LOCUS       DASHWH010000222          514 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.234, whole genome shotgun sequence.
ACCESSION   DASHWH010000222 DASHWH010000000
VERSION     DASHWH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.234"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          156 a           76 c           78 g          204 t
ORIGIN      
        1 ttatttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata
       61 tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata
      121 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
      181 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
      241 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
      301 cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac
      361 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
      421 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
      481 ttaaataatt taattttttt gttcctattt attt
//
LOCUS       DASHWH010000223          510 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.235, whole genome shotgun sequence.
ACCESSION   DASHWH010000223 DASHWH010000000
VERSION     DASHWH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.235"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..508
                     /locus_tag="U2R41_002995"
     CDS             <1..508
                     /locus_tag="U2R41_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEM8890307.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DASHWH010000224          504 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.236, whole genome shotgun sequence.
ACCESSION   DASHWH010000224 DASHWH010000000
VERSION     DASHWH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.236"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DASHWH010000225          500 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.237, whole genome shotgun sequence.
ACCESSION   DASHWH010000225 DASHWH010000000
VERSION     DASHWH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.237"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>500
                     /locus_tag="U2R41_002996"
     CDS             <1..>500
                     /locus_tag="U2R41_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HEM8890308.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DASHWH010000226          498 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.238, whole genome shotgun sequence.
ACCESSION   DASHWH010000226 DASHWH010000000
VERSION     DASHWH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.238"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            47..211
                     /locus_tag="U2R41_002997"
     CDS             47..211
                     /locus_tag="U2R41_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890309.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            300..>498
                     /locus_tag="U2R41_002998"
     CDS             300..>498
                     /locus_tag="U2R41_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEM8890310.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIE"
BASE COUNT          175 a           73 c          120 g          130 t
ORIGIN      
        1 gagaatttag ctgtagcgag attggtggat atggaggtta taaacaatga agtttaaaat
       61 ctttgaagag gacactcgtt ataaattaga aaaagaatta aacgattttg cgaaaaacaa
      121 tgagattcag catatatctt tagcaacttc taagagaggt tatgcaaatt actatgcagc
      181 tgttgtgagc tatgtaagtc gagaagtgta actcggcaaa taaaaaaagc cggatcgctc
      241 cgactgattc aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga
      301 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
      361 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
      421 ttattacgga tatgccgaag gcaccgaagc acggcaataa ggcagaagac gcgatcattc
      481 aaatgatgga tatagaag
//
LOCUS       DASHWH010000227          491 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.239, whole genome shotgun sequence.
ACCESSION   DASHWH010000227 DASHWH010000000
VERSION     DASHWH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.239"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>491
                     /locus_tag="U2R41_002999"
     CDS             <1..>491
                     /locus_tag="U2R41_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8890311.1"
                     /translation="PKITKEKVPAKKMNELTVQEELRSYELPEQQEKEIKVVSQSEFD
                     QLNLPQSTLQLVQVKDFQANLKPIETLVAQNKMNNPSLQKVEYSNQKYEMYEVYNGLF
                     SGFEFMGFFLGLAFLTMLASCLMFKILSGSKSDIARYEMLEKIGTRRGLLKQSIRREV
                     GVL"
BASE COUNT          175 a           86 c          102 g          128 t
ORIGIN      
        1 gccgaagatc acgaaagaaa aagttcctgc taaaaaaatg aatgaactga ctgtacaaga
       61 ggaacttcga agctatgaat tgccggaaca gcaagaaaaa gaaatcaagg tagtcagtca
      121 atcagaattt gatcaactga acttgccaca atccacttta cagctagttc aggtaaaaga
      181 cttccaagca aacctgaaac caatcgaaac acttgttgct caaaataaaa tgaacaatcc
      241 atctttgcaa aaagtagagt acagcaacca gaaatatgaa atgtatgaag tgtataatgg
      301 tctattctct ggttttgaat ttatgggatt cttcctaggt ttagctttct tgaccatgct
      361 tgctagctgt ttgatgttca agatcttgtc aggaagcaag agtgacattg ctcgttatga
      421 aatgcttgaa aagataggaa cacgacgcgg gttgcttaaa caatcgatcc gtagagaagt
      481 aggcgtgtta t
//
LOCUS       DASHWH010000228          487 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.240, whole genome shotgun sequence.
ACCESSION   DASHWH010000228 DASHWH010000000
VERSION     DASHWH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.240"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..413)
                     /locus_tag="U2R41_003000"
     CDS             complement(<1..413)
                     /locus_tag="U2R41_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890312.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          184 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacat
//
LOCUS       DASHWH010000229          462 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.241, whole genome shotgun sequence.
ACCESSION   DASHWH010000229 DASHWH010000000
VERSION     DASHWH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.241"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..430)
                     /locus_tag="U2R41_003001"
     CDS             complement(<1..430)
                     /locus_tag="U2R41_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8890313.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DASHWH010000230          453 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.242, whole genome shotgun sequence.
ACCESSION   DASHWH010000230 DASHWH010000000
VERSION     DASHWH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.242"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>453
                     /locus_tag="U2R41_003002"
     CDS             <1..>453
                     /locus_tag="U2R41_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890314.1"
                     /translation="IESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPIT
                     ADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWK
                     EKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          183 a           67 c          105 g           98 t
ORIGIN      
        1 tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa
       61 acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac
      121 tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa
      181 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
      241 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      301 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      361 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      421 tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DASHWH010000231          452 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.243, whole genome shotgun sequence.
ACCESSION   DASHWH010000231 DASHWH010000000
VERSION     DASHWH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.243"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            98..>452
                     /locus_tag="U2R41_003003"
     CDS             98..>452
                     /locus_tag="U2R41_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEM8890315.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWS"
BASE COUNT          155 a           93 c           77 g          127 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tg
//
LOCUS       DASHWH010000232          433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.244, whole genome shotgun sequence.
ACCESSION   DASHWH010000232 DASHWH010000000
VERSION     DASHWH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.244"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>433)
                     /locus_tag="U2R41_003004"
     CDS             complement(<1..>433)
                     /locus_tag="U2R41_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890316.1"
                     /translation="SIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFE
                     EKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANK
                     HKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT          124 a           89 c           80 g          140 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaacaccgtc
      241 gtttctctaa atcggcgaac ataattcttt ccaaaagttg agaaatgggg gattttctcc
      301 tcgaaggagt agcctaagaa ccagcggtaa gcgacattgg tatcgacttc cttaatggtt
      361 tgtcgcatgg aacggatacc gaataaatac tgaataaaca ccaatttgat cagaatcact
      421 ggatcgatac ttg
//
LOCUS       DASHWH010000233          423 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.245, whole genome shotgun sequence.
ACCESSION   DASHWH010000233 DASHWH010000000
VERSION     DASHWH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.245"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..423
                     /locus_tag="U2R41_003005"
                     /pseudo
     CDS             55..423
                     /locus_tag="U2R41_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a           69 c           81 g          104 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atc
//
LOCUS       DASHWH010000234          381 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.246, whole genome shotgun sequence.
ACCESSION   DASHWH010000234 DASHWH010000000
VERSION     DASHWH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.246"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          164 a           50 c           38 g          129 t
ORIGIN      
        1 aaacaaaaat aaataaagtc ggatttttct gataattcaa cgcaaaaaaa cgaacaattt
       61 ttcaataaat cagtcttcta aaatagataa cctaaaaaaa acgaacaaac aaataaaatg
      121 actgtccttc attcactaaa catattttta ttttttatat tgttaaaaat tcacaattat
      181 tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca
      241 tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt
      301 cccatattgt gcaaaaaaat acaaggtgga ggctctacta tgattgaaga atatattgag
      361 aaaaatattt taagacagct g
//
LOCUS       DASHWH010000235          362 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.247, whole genome shotgun sequence.
ACCESSION   DASHWH010000235 DASHWH010000000
VERSION     DASHWH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.247"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>362)
                     /locus_tag="U2R41_003006"
     rRNA            complement(<1..>362)
                     /locus_tag="U2R41_003006"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DASHWH010000236          362 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.248, whole genome shotgun sequence.
ACCESSION   DASHWH010000236 DASHWH010000000
VERSION     DASHWH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.248"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>362
                     /locus_tag="U2R41_003007"
     CDS             <1..>362
                     /locus_tag="U2R41_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEM8890317.1"
                     /translation="MQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSY
                     WQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSW
                     FESIKDILKSIDPGGVMLA"
BASE COUNT          130 a           44 c           82 g          106 t
ORIGIN      
        1 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
       61 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      121 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      181 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      241 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      301 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      361 gc
//
LOCUS       DASHWH010000237          361 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.249, whole genome shotgun sequence.
ACCESSION   DASHWH010000237 DASHWH010000000
VERSION     DASHWH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.249"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DASHWH010000238          349 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.250, whole genome shotgun sequence.
ACCESSION   DASHWH010000238 DASHWH010000000
VERSION     DASHWH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.250"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(44..>349)
                     /locus_tag="U2R41_003008"
     CDS             complement(44..>349)
                     /locus_tag="U2R41_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8890318.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DASHWH010000239          337 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.251, whole genome shotgun sequence.
ACCESSION   DASHWH010000239 DASHWH010000000
VERSION     DASHWH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.251"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            122..328
                     /locus_tag="U2R41_003009"
     CDS             122..328
                     /locus_tag="U2R41_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8890319.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DASHWH010000240          332 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.252, whole genome shotgun sequence.
ACCESSION   DASHWH010000240 DASHWH010000000
VERSION     DASHWH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.252"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>332
                     /locus_tag="U2R41_003010"
     CDS             <1..>332
                     /locus_tag="U2R41_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890320.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DASHWH010000241          316 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.253, whole genome shotgun sequence.
ACCESSION   DASHWH010000241 DASHWH010000000
VERSION     DASHWH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.253"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>316
                     /locus_tag="U2R41_003011"
     CDS             <1..>316
                     /locus_tag="U2R41_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8890321.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSH"
BASE COUNT           80 a           66 c           83 g           87 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatc
//
LOCUS       DASHWH010000242          309 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.254, whole genome shotgun sequence.
ACCESSION   DASHWH010000242 DASHWH010000000
VERSION     DASHWH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.254"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..>309
                     /locus_tag="U2R41_003012"
     CDS             51..>309
                     /locus_tag="U2R41_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8890322.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DASHWH010000243          306 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.denovo.255, whole genome shotgun sequence.
ACCESSION   DASHWH010000243 DASHWH010000000
VERSION     DASHWH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.denovo.255"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            84..272
                     /locus_tag="U2R41_003013"
     CDS             84..272
                     /locus_tag="U2R41_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890323.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          114 a           54 c           48 g           90 t
ORIGIN      
        1 caatttaaac atttgacaaa ctatagaaca gcacttaaaa tgtgtataca agtgacagcg
       61 ctttaaaaaa agaggtgcag cctatgttaa aaaaaactat gttggtacta ggttctattt
      121 ctctaggagt attaggtttt agccccatgc tattatccgt aattggaaaa aataattcca
      181 aaggggaact taaattaaat ccaaattact acaatagtcc taatgaacct atgtttattt
      241 cagaaaaaca aaacgacggt caggacaaat aatcctgccg tcgttttgct ctaacttcta
      301 ccagta
//
LOCUS       DASHWH010000244        22704 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.042, whole genome shotgun sequence.
ACCESSION   DASHWH010000244 DASHWH010000000
VERSION     DASHWH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.042"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..212
                     /locus_tag="U2R41_003014"
     CDS             <1..212
                     /locus_tag="U2R41_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8890324.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            447..683
                     /locus_tag="U2R41_003015"
     CDS             447..683
                     /locus_tag="U2R41_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890325.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="U2R41_003016"
     CDS             690..2642
                     /locus_tag="U2R41_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEM8890326.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="U2R41_003017"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="U2R41_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEM8890327.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3573..4613
                     /locus_tag="U2R41_003018"
     CDS             3573..4613
                     /locus_tag="U2R41_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8890328.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4711..4899
                     /locus_tag="U2R41_003019"
     CDS             4711..4899
                     /locus_tag="U2R41_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM8890329.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4957..5310)
                     /locus_tag="U2R41_003020"
     CDS             complement(4957..5310)
                     /locus_tag="U2R41_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890330.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5841..6263
                     /locus_tag="U2R41_003021"
     CDS             5841..6263
                     /locus_tag="U2R41_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8890331.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6260..6490
                     /locus_tag="U2R41_003022"
     CDS             6260..6490
                     /locus_tag="U2R41_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8890332.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6988..7188
                     /locus_tag="U2R41_003023"
     CDS             6988..7188
                     /locus_tag="U2R41_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM8890333.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7215..8408
                     /locus_tag="U2R41_003024"
     CDS             7215..8408
                     /locus_tag="U2R41_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8890334.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="U2R41_003025"
     CDS             complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="U2R41_003025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM8890335.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10100..11023)
                     /locus_tag="U2R41_003026"
     CDS             complement(10100..11023)
                     /locus_tag="U2R41_003026"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM8890336.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11219..11824
                     /locus_tag="U2R41_003027"
     CDS             11219..11824
                     /locus_tag="U2R41_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8890337.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12132..13148
                     /locus_tag="U2R41_003028"
     CDS             12132..13148
                     /locus_tag="U2R41_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8890338.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13289..14590
                     /locus_tag="U2R41_003029"
     CDS             13289..14590
                     /locus_tag="U2R41_003029"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM8890339.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14636..15298
                     /gene="deoC"
                     /locus_tag="U2R41_003030"
     CDS             14636..15298
                     /gene="deoC"
                     /locus_tag="U2R41_003030"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM8890340.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15319..15717
                     /locus_tag="U2R41_003031"
     CDS             15319..15717
                     /locus_tag="U2R41_003031"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM8890341.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15783..16865
                     /locus_tag="U2R41_003032"
     CDS             15783..16865
                     /locus_tag="U2R41_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8890342.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17077..18639
                     /locus_tag="U2R41_003033"
     CDS             17077..18639
                     /locus_tag="U2R41_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8890343.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18632..19765
                     /locus_tag="U2R41_003034"
     CDS             18632..19765
                     /locus_tag="U2R41_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8890344.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19765..20721
                     /locus_tag="U2R41_003035"
     CDS             19765..20721
                     /locus_tag="U2R41_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8890345.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20820..21986
                     /gene="deoB"
                     /locus_tag="U2R41_003036"
     CDS             20820..21986
                     /gene="deoB"
                     /locus_tag="U2R41_003036"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM8890346.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22010..>22704
                     /locus_tag="U2R41_003037"
     CDS             22010..>22704
                     /locus_tag="U2R41_003037"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8890347.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGIS"
BASE COUNT         7379 a         4026 c         4720 g         6579 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     4681 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     4741 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     4801 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     4861 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     4921 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     4981 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     5041 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     5101 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     5161 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     5221 ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct
     5281 aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc
     5341 ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa
     5401 cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat
     5461 ttacctatac aactttataa aatttagtca tactcctaga aagtagataa aattggcgac
     5521 ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct cttaattaag
     5581 ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa tgtggttttg
     5641 cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct gtttatgtgc
     5701 ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca acaaatcccc
     5761 cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact ccctttcacg
     5821 acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata gaaaatcagt
     5881 ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc aaacatctag
     5941 acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt gtcaactctt
     6001 tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt ggattaagaa
     6061 tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag aaaaaacgga
     6121 acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg gagttactaa
     6181 atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg attaaagaat
     6241 tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc ccctcattgt
     6301 aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt atcgagggta
     6361 tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc atatggttat
     6421 tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt tatcgttcga
     6481 aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc ttcccgattt
     6541 gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga taggctaaca
     6601 agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt aagaaaagag
     6661 ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt agtaattcga
     6721 aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga tactcccgta
     6781 tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca tgagaaccat
     6841 gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga tattcttctc
     6901 ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt ggagttttcc
     6961 tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga tttagttgag
     7021 ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga attgatgatt
     7081 acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg tgaagctgtt
     7141 gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt tttgcacagt
     7201 aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga acctattatt
     7261 ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat cgtagtggat
     7321 tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg aaaccagaag
     7381 aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa attttccttc
     7441 catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca actattaaga
     7501 agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt gatgtgcaaa
     7561 attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct gttcaaggac
     7621 tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca aatccatcta
     7681 agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca aaagaagaat
     7741 ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt ttatttatct
     7801 ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc attcaatggg
     7861 aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat tacaagaatc
     7921 aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat attgtattag
     7981 ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat ctaattcaga
     8041 cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta tcatatgcta
     8101 ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg ttacggcatt
     8161 ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa gatcgtctag
     8221 gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct aacagtaatt
     8281 tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa caaaattatg
     8341 atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa atgaaaaaag
     8401 tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat aagaggattt
     8461 ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag tgatgtcata
     8521 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
     8581 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
     8641 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
     8701 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
     8761 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
     8821 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
     8881 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
     8941 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
     9001 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
     9061 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
     9121 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
     9181 tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
     9241 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
     9301 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
     9361 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
     9421 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
     9481 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
     9541 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
     9601 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
     9661 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
     9721 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
     9781 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
     9841 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
     9901 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
     9961 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
    10021 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
    10081 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
    10141 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
    10201 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
    10261 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
    10321 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
    10381 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
    10441 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
    10501 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
    10561 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
    10621 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
    10681 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
    10741 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
    10801 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
    10861 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
    10921 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
    10981 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
    11041 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
    11101 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
    11161 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
    11221 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
    11281 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
    11341 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
    11401 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
    11461 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
    11521 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
    11581 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
    11641 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
    11701 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
    11761 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
    11821 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
    11881 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
    11941 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
    12001 tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
    12061 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
    12121 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
    12181 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
    12241 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
    12301 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
    12361 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
    12421 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
    12481 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
    12541 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
    12601 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
    12661 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
    12721 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
    12781 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
    12841 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
    12901 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
    12961 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
    13021 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
    13081 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
    13141 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
    13201 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
    13261 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
    13321 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
    13381 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
    13441 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
    13501 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
    13561 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
    13621 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
    13681 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    13741 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    13801 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    13861 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    13921 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    13981 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    14041 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    14101 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    14161 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    14221 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    14281 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    14341 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    14401 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    14461 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    14521 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    14581 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    14641 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    14701 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    14761 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    14821 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    14881 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    14941 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    15001 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    15061 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    15121 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    15181 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    15241 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    15301 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    15361 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    15421 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    15481 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    15541 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    15601 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    15661 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    15721 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    15781 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    15841 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    15901 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    15961 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    16021 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    16081 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    16141 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    16201 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    16261 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    16321 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    16381 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    16441 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    16501 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    16561 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    16621 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    16681 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    16741 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    16801 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    16861 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    16921 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    16981 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    17041 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    17101 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    17161 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    17221 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    17281 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    17341 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    17401 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    17461 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    17521 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    17581 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    17641 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    17701 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    17761 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    17821 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    17881 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    17941 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    18001 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    18061 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    18121 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    18181 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    18241 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    18301 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    18361 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    18421 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    18481 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    18541 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    18601 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    18661 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    18721 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    18781 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    18841 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    18901 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    18961 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    19021 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    19081 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    19141 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    19201 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    19261 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    19321 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    19381 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    19441 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    19501 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    19561 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    19621 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    19681 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    19741 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    19801 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    19861 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    19921 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    19981 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    20041 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    20101 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    20161 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    20221 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    20281 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    20341 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    20401 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    20461 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    20521 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    20581 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    20641 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    20701 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    20761 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    20821 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    20881 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    20941 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    21001 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    21061 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    21121 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    21181 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    21241 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    21301 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    21361 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    21421 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    21481 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    21541 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    21601 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    21661 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    21721 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    21781 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    21841 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    21901 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    21961 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    22021 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    22081 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    22141 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    22201 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    22261 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    22321 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    22381 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    22441 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    22501 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    22561 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    22621 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    22681 cggattaaaa gtgttaggaa tctc
//
LOCUS       DASHWH010000245        20985 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.047, whole genome shotgun sequence.
ACCESSION   DASHWH010000245 DASHWH010000000
VERSION     DASHWH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.047"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(134..577)
                     /locus_tag="U2R41_003038"
     CDS             complement(134..577)
                     /locus_tag="U2R41_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM8890348.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U2R41_003039"
     CDS             complement(795..2135)
                     /locus_tag="U2R41_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8890349.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U2R41_003040"
     CDS             2404..3300
                     /locus_tag="U2R41_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM8890350.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U2R41_003041"
     CDS             complement(3450..4391)
                     /locus_tag="U2R41_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM8890351.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U2R41_003042"
     CDS             4517..5638
                     /locus_tag="U2R41_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8890352.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2R41_003043"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2R41_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM8890353.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U2R41_003044"
     CDS             7426..7992
                     /locus_tag="U2R41_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8890354.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="U2R41_003045"
     CDS             8203..9264
                     /locus_tag="U2R41_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8890355.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="U2R41_003046"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="U2R41_003046"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM8890356.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="U2R41_003047"
     CDS             10177..12159
                     /locus_tag="U2R41_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8890357.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2R41_003048"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2R41_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8890358.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="U2R41_003049"
     CDS             12881..14305
                     /locus_tag="U2R41_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8890359.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="U2R41_003050"
     CDS             complement(14486..15259)
                     /locus_tag="U2R41_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8890360.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="U2R41_003051"
     CDS             complement(15256..16161)
                     /locus_tag="U2R41_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8890361.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="U2R41_003052"
     CDS             complement(16315..16635)
                     /locus_tag="U2R41_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM8890362.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="U2R41_003053"
     CDS             17109..17795
                     /locus_tag="U2R41_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM8890363.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="U2R41_003054"
     CDS             17797..18741
                     /locus_tag="U2R41_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM8890364.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="U2R41_003055"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="U2R41_003055"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM8890365.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="U2R41_003056"
     CDS             19663..20940
                     /locus_tag="U2R41_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890366.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DASHWH010000246        20862 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.048, whole genome shotgun sequence.
ACCESSION   DASHWH010000246 DASHWH010000000
VERSION     DASHWH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.048"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            267..1304
                     /locus_tag="U2R41_003057"
     CDS             267..1304
                     /locus_tag="U2R41_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8890367.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="U2R41_003058"
     CDS             complement(1393..1716)
                     /locus_tag="U2R41_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM8890368.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="U2R41_003059"
     CDS             complement(1703..2410)
                     /locus_tag="U2R41_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM8890369.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="U2R41_003060"
     CDS             2496..2837
                     /locus_tag="U2R41_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEM8890370.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="U2R41_003061"
     CDS             complement(3008..3856)
                     /locus_tag="U2R41_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890371.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="U2R41_003062"
     CDS             4454..5317
                     /locus_tag="U2R41_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8890372.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="U2R41_003063"
     CDS             5417..5899
                     /locus_tag="U2R41_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEM8890373.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="U2R41_003064"
     CDS             complement(6162..6629)
                     /locus_tag="U2R41_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM8890374.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="U2R41_003065"
     CDS             6752..7603
                     /locus_tag="U2R41_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8890375.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="U2R41_003066"
     CDS             complement(7854..9812)
                     /locus_tag="U2R41_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8890376.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="U2R41_003067"
     CDS             complement(9809..10681)
                     /locus_tag="U2R41_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8890377.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="U2R41_003068"
     CDS             complement(11023..11895)
                     /locus_tag="U2R41_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8890378.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="U2R41_003069"
     CDS             12023..12592
                     /locus_tag="U2R41_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM8890379.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="U2R41_003070"
     CDS             12659..13396
                     /locus_tag="U2R41_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8890380.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="U2R41_003071"
     CDS             13627..14895
                     /locus_tag="U2R41_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8890381.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="U2R41_003072"
     CDS             15382..15846
                     /locus_tag="U2R41_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8890382.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="U2R41_003073"
     CDS             15830..16459
                     /locus_tag="U2R41_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8890383.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="U2R41_003074"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="U2R41_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM8890384.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="U2R41_003075"
     CDS             18338..18784
                     /locus_tag="U2R41_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM8890385.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="U2R41_003076"
     CDS             complement(18983..19498)
                     /locus_tag="U2R41_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8890386.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="U2R41_003077"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="U2R41_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6632 a         3703 c         4314 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatat tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DASHWH010000247         7482 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.105, whole genome shotgun sequence.
ACCESSION   DASHWH010000247 DASHWH010000000
VERSION     DASHWH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.105"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..192
                     /locus_tag="U2R41_003078"
     CDS             109..192
                     /locus_tag="U2R41_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM8890387.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="U2R41_003079"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="U2R41_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM8890388.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="U2R41_003080"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="U2R41_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="U2R41_003081"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="U2R41_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="U2R41_003082"
     CDS             2348..2593
                     /locus_tag="U2R41_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8890389.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="U2R41_003083"
     CDS             2696..3565
                     /locus_tag="U2R41_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM8890390.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="U2R41_003084"
     CDS             3546..4280
                     /locus_tag="U2R41_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8890391.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="U2R41_003085"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="U2R41_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="U2R41_003086"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="U2R41_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM8890392.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="U2R41_003087"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="U2R41_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM8890393.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6927..7469
                     /locus_tag="U2R41_003088"
     CDS             6927..7469
                     /locus_tag="U2R41_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8890394.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DASHWH010000248         6372 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.111, whole genome shotgun sequence.
ACCESSION   DASHWH010000248 DASHWH010000000
VERSION     DASHWH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.111"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2R41_003089"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2R41_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8890395.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2R41_003090"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2R41_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8890396.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="U2R41_003091"
     CDS             3976..5238
                     /locus_tag="U2R41_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8890397.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..6370
                     /locus_tag="U2R41_003092"
     CDS             5483..6370
                     /locus_tag="U2R41_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8890398.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         2211 a          903 c         1312 g         1946 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc
     6181 ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga
     6241 agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat
     6301 ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca aagttttaaa
     6361 taaagaataa aa
//
LOCUS       DASHWH010000249         6372 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.112, whole genome shotgun sequence.
ACCESSION   DASHWH010000249 DASHWH010000000
VERSION     DASHWH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.112"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2R41_003093"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2R41_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8890399.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2R41_003094"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2R41_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8890400.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="U2R41_003095"
     CDS             3976..5238
                     /locus_tag="U2R41_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8890401.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..6370
                     /locus_tag="U2R41_003096"
     CDS             5483..6370
                     /locus_tag="U2R41_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8890402.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         2210 a          903 c         1313 g         1946 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc
     6181 ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga
     6241 agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat
     6301 ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca aagttttaaa
     6361 taaagaataa aa
//
LOCUS       DASHWH010000250         4995 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.127, whole genome shotgun sequence.
ACCESSION   DASHWH010000250 DASHWH010000000
VERSION     DASHWH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.127"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(30..2180)
                     /locus_tag="U2R41_003097"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="U2R41_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="U2R41_003098"
     CDS             2326..2901
                     /locus_tag="U2R41_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8890403.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="U2R41_003099"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="U2R41_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM8890404.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="U2R41_003100"
     CDS             3788..4942
                     /locus_tag="U2R41_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM8890405.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DASHWH010000251         4786 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.131, whole genome shotgun sequence.
ACCESSION   DASHWH010000251 DASHWH010000000
VERSION     DASHWH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.131"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            285..1196
                     /locus_tag="U2R41_003101"
     CDS             285..1196
                     /locus_tag="U2R41_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM8890406.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1349..1834
                     /gene="vanZ"
                     /locus_tag="U2R41_003102"
     CDS             1349..1834
                     /gene="vanZ"
                     /locus_tag="U2R41_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEM8890407.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2121..2450)
                     /locus_tag="U2R41_003103"
     CDS             complement(2121..2450)
                     /locus_tag="U2R41_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8890408.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2755..3084
                     /locus_tag="U2R41_003104"
     CDS             2755..3084
                     /locus_tag="U2R41_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8890409.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3074..3361
                     /locus_tag="U2R41_003105"
     CDS             3074..3361
                     /locus_tag="U2R41_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8890410.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3640..4203)
                     /locus_tag="U2R41_003106"
     CDS             complement(3640..4203)
                     /locus_tag="U2R41_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8890411.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1682 a          722 c          813 g         1569 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc atagaggggt ggtatttcac
       61 accgcccatt gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga
      121 aaattcatct acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt
      181 tacggggcaa aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga
      241 aatatttcat acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt
      301 ttttattgtt attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt
      361 tttctcagga aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa
      421 atagtgggac ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag
      481 gaaatctgct attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata
      541 tcgtgaattt atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata
      601 tttatatgtc aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg
      661 gtggcgttag tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc
      721 tttaccaaga aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag
      781 gtttatcact agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt
      841 ggatagaaga aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag
      901 agttaacagg aattcaatat gaaccatggc atattcgcta tgttggttta ccacatagtg
      961 cgattatgaa agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa
     1021 aaaccatttc tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta
     1081 aaaataccac cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag
     1141 acggtgtaat tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat
     1201 ggcggaatga aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa
     1261 cgttagcttt ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac
     1321 ttaaacaaaa gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc
     1381 tttatattta gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc
     1441 agtatttaat tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt
     1501 cagagagatg atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa
     1561 ttttaaagaa atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt
     1621 tgaaataatt caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa
     1681 tactgttgga ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca
     1741 aaaaaaatta gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt
     1801 ttaccgtacc catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct
     1861 ttcatacaca taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac
     1921 tcgcccgtag gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac
     1981 tatagtaaca aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata
     2041 aatttcctta acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat
     2101 agatagattt tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct
     2161 aaagagtaat aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa
     2221 aaacgtaaat ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag
     2281 acgcacattt ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg
     2341 aaacatttcc ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt
     2401 ccatttactt tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc
     2461 tcacattcaa acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta
     2521 ccttaattcc aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag
     2581 tgcgtttttg attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata
     2641 aaactccttt gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag
     2701 cgtacaatgt aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt
     2761 acaaaaacaa gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg
     2821 cccaatggtg ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta
     2881 gagcctaaaa aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa
     2941 ggttatagca aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct
     3001 gcatttaaat ctattgctga agatactgtt gcaaattcta aaccgatgac aaaagaggag
     3061 ttggcagcag aaattggctt atgagattct accttctaaa cacgtaataa agtatctaaa
     3121 aaaattaaaa gagaaaacat taaaagaaca atttttaacg ataatttatg atgagatagc
     3181 agttcgtcct cattcgggag aacaaaaaac gggtgattta tcaggaattt gggctattgg
     3241 attcaagtat gcaggtacga catatcgcgt tgcatatgaa attaaagata atgcagtgat
     3301 acctatctta ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa agattaggta
     3361 agaacccaat gtttcaactt gagaggatag acgacacttt aaaagataat ttttaacagc
     3421 tgagagttgt aataccctat aattttaaac cactaaccta tatatttatg aaaaatagaa
     3481 aaaattaaaa cgcattttaa gcctctttca aattaaaagc ttcatcctta tgcacaaaga
     3541 gggcttaaaa tgcgttattt tatattgttt tttagtaata ataaaaaaat cactctaatt
     3601 ctcccataaa tattatagat tcctaaagat taaaatagat tattccctat tgtcttttaa
     3661 gtagcggtag agtgttgcct tgctaatacc agtcatttcc gtaatttctt ttacgctata
     3721 aacctgactt ttatacaatt ttactgctcg atcaacatct ttctggttca ctcgtggacg
     3781 gccaccttta cgacctctcg cacgtgcact tttcaaacct tctttggtac gttcaacaat
     3841 taaatcacgt tcgaactgac taaatgcttg gaagacagtc atcattaagc gtccgtgagg
     3901 cgtgctggta tcgaagtttt ctttcaagct caccagctta acatggtgtt cttcaaaata
     3961 ggtaacaagg tcaatcaaat cctttgtact acgacccaat cgagaaaaac tttctacaat
     4021 taccgtatct cccggacgaa ttttatcttt taaacgattt agttggggac gaaccgcttt
     4081 ggttcccgtc attttctcag ttaggatttc attacaattt tgttcattga gtaagtctaa
     4141 ttgacgattt agttcttgtg aacgtgtact cactcgggca taaccataaa tgtatccact
     4201 catgcttttc ttcctttcgt actttaattc tggcttgatt gtatcataaa agggagttat
     4261 agatacatag ttaatgcaac aagttatgat acaaaatatg acctctttta tttaataaaa
     4321 gaggtcattt cattttgtct cataatcgat tgtttttgat acgagtgtgt atacgaaata
     4381 aacaaaaaaa tcttaagtaa atttctagat acctaattta atcctaacta gtgtatccta
     4441 aagttaataa cgagtttatc acaatgaaaa ttataaaatg ctcccatatt agatataaca
     4501 tgcaatattt gaattagaac tacaaaattt attcgtttta taaagtaaat aatgttgaga
     4561 tactaaggaa tagaatatta taaggaattg agctgaatgt attggataaa aaaagaataa
     4621 ctttattaga gatggactat aaaaatgctc gtaattttct tctgaagtct gaaagctatt
     4681 tgaatgctca gctaccagaa tatattagtt ttgacgaaac tttaaaatct gctaaaaaag
     4741 ttttaacttc gccgtttgta taattaagcg agacaaataa aaaagc
//
LOCUS       DASHWH010000252         2999 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.160, whole genome shotgun sequence.
ACCESSION   DASHWH010000252 DASHWH010000000
VERSION     DASHWH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.160"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            245..1213
                     /locus_tag="U2R41_003107"
     CDS             245..1213
                     /locus_tag="U2R41_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM8890412.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="U2R41_003108"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="U2R41_003108"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM8890413.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="U2R41_003109"
     CDS             2243..2851
                     /locus_tag="U2R41_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM8890414.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          809 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DASHWH010000253         2565 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.172, whole genome shotgun sequence.
ACCESSION   DASHWH010000253 DASHWH010000000
VERSION     DASHWH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.172"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..341)
                     /locus_tag="U2R41_003110"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U2R41_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="U2R41_003111"
     CDS             complement(1055..1345)
                     /locus_tag="U2R41_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8890415.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2244)
                     /locus_tag="U2R41_003112"
                     /pseudo
     CDS             complement(1553..2244)
                     /locus_tag="U2R41_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          759 a          472 c          356 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgatttc tacatttgtt ttttcttgtg tcagtgttgc
     1741 gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc
     1801 ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat
     1861 ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac
     1921 taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc
     1981 atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt
     2041 ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca atgtatgatc
     2101 aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat
     2161 ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa cgacaatccc
     2221 accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt ttacatgcta
     2281 aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt aattttaata
     2341 taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa atctttttta
     2401 tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac aaaaacaaaa
     2461 aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt tattacaaaa
     2521 aacgcggatt tgtcataaat tgtcatgctt cttttaccta tgaaa
//
LOCUS       DASHWH010000254         2092 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.187, whole genome shotgun sequence.
ACCESSION   DASHWH010000254 DASHWH010000000
VERSION     DASHWH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.187"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..823)
                     /locus_tag="U2R41_003113"
     CDS             complement(<1..823)
                     /locus_tag="U2R41_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8890416.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASV"
     gene            1376..1924
                     /gene="aac(6')"
                     /locus_tag="U2R41_003114"
     CDS             1376..1924
                     /gene="aac(6')"
                     /locus_tag="U2R41_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM8890417.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          677 a          368 c          415 g          632 t
ORIGIN      
        1 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
       61 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
      121 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
      181 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
      241 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
      301 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
      361 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
      421 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
      481 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
      541 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
      601 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
      661 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
      721 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
      781 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
      841 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
      901 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
      961 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
     1021 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
     1081 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
     1141 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
     1201 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
     1261 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
     1321 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
     1381 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
     1441 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
     1501 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
     1561 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
     1621 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
     1681 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
     1741 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
     1801 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
     1861 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
     1921 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
     1981 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
     2041 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aa
//
LOCUS       DASHWH010000255         1467 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE37959
            SAMN38764585-rid20551843.guided.200, whole genome shotgun sequence.
ACCESSION   DASHWH010000255 DASHWH010000000
VERSION     DASHWH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764585
            Sequence Read Archive: SRR27176739
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024148.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:28:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,049
            Genes (coding)                    :: 2,971
            CDSs (with protein)               :: 2,971
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 37 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 11 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764585-rid20551843.guided.200"
                     /strain="VRE37959"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1348
                     /locus_tag="U2R41_003115"
     CDS             <1..1348
                     /locus_tag="U2R41_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEM8890418.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          246 g          482 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgtt
//
